RLG00000025415

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0019739
Physical Location & Seq
Reverse (-)
45642412 .. 45642876
465 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025415

Sequence Viewer

Length: 465 bp
ATGCGGGCCTACACAGATTACAATACTCTTCTGCGGGGATCCACTGTATCCTCCGCTGCTCCTGCTTGGGATTTGCGCCACGACAGCGTTTGTCAGCATGTGGATGCACAACGTGTCAGCTGCGGTGATGATGATGCCGGTGGCGACCGGGATTTTGCATCGTTTTCCGGTGGGTCTCGACTAGTCCGTCGTGTCCAGCAAGTTTTGCAAAGCTGTGGTTCTGGGGGTGACGTACTTGGCTGCCATCGGAAGGATGAGTACTCTGACAGGGACAGGTGTCAATCTGATATTGGTTGGAATGTGGAAGAGCTATTTTCCAGAGGAGGAGCCGATCAGCTTCAGTACTTGGTTCCTCTTTGGGTTTCCCATGGCTTTGTTGATTTTCCTGGCCTTGTGGATCATATGCTTTGCTGCTTGTATTGCTCCAAAAGTTCAGGACAAGGCCCATTTGAAGAGGGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

155

Amino Acids

16.87

Weight (kDa)

4.91

Isoelectric Point (pI)

46.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019739)

Species Orthologous Gene IDs
malus_domestica MD10G1332700.v1.1 MD10G1333600.v1.1
pyrus_communis pycom10g28290
rosa_laevigata RLG00000025412 RLG00000025415
rosa_roxburghii Rroxscaffold_6G00424760
rosa_samantha Rh5BG013200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 186
AciI CCGC 4 cut(s) 4, 34, 54, 123
AclWI GGATC 3 cut(s) 33, 46, 405
AcuI CTGAAG 1 cut(s) 323
AdeI CACNNNGTG 1 cut(s) 113
AfaI GTAC 3 cut(s) 234, 260, 344
AfiI CCNNNNNNNGG 1 cut(s) 250
AflIII ACRYGT 1 cut(s) 112
AgsI TTSAA 1 cut(s) 452
AhlI ACTAGT 1 cut(s) 181
AjnI CCWGG 1 cut(s) 385
AluBI AGCT 5 cut(s) 120, 213, 310, 337, 461
AluI AGCT 5 cut(s) 120, 213, 310, 337, 461
Alw26I GTCTC 1 cut(s) 180
AlwI GGATC 3 cut(s) 33, 46, 405
AoxI GGCC 3 cut(s) 6, 388, 442
ApeKI GCWGC 4 cut(s) 56, 120, 240, 411
ArsI GACNNNNNNTTYG 2 cut(s) 137, 169
AspLEI GCGC 1 cut(s) 78
AspS9I GGNCC 2 cut(s) 6, 443
AsuC2I CCSGG 1 cut(s) 149
AsuHPI GGTGA 2 cut(s) 137, 239
BamHI GGATCC 1 cut(s) 38
BarI GAAGNNNNNNTAC 2 cut(s) 242, 274
BbvI GCAGC 4 cut(s) 43, 107, 227, 398
BccI CCATC 1 cut(s) 252
BciT130I CCWGG 1 cut(s) 387
BciVI GTATCC 1 cut(s) 58
BcnI CCSGG 1 cut(s) 149
BcoDI GTCTC 1 cut(s) 180
BcuI ACTAGT 1 cut(s) 181
BfaI CTAG 1 cut(s) 182
BfuI GTATCC 1 cut(s) 58
BisI GCNGC 4 cut(s) 57, 121, 241, 412
BlsI GCNGC 4 cut(s) 58, 122, 242, 413
BmcAI AGTACT 2 cut(s) 260, 344
Bme1390I CCNGG 2 cut(s) 149, 387
BmgT120I GGNCC 2 cut(s) 6, 443
BmiI GGNNCC 3 cut(s) 40, 328, 351
BmrFI CCNGG 2 cut(s) 149, 387
BmsI GCATC 3 cut(s) 94, 124, 167
BoxI GACNNNNGTC 1 cut(s) 276
BpuMI CCSGG 1 cut(s) 149
BsaI GGTCTC 1 cut(s) 180
BsaJI CCNNGG 1 cut(s) 367
BsaWI WCCGGW 1 cut(s) 167
Bsc4I CCNNNNNNNGG 1 cut(s) 250
Bse118I RCCGGY 1 cut(s) 137
BseBI CCWGG 1 cut(s) 387
BseDI CCNNGG 1 cut(s) 367
BseGI GGATG 2 cut(s) 109, 259
BseLI CCNNNNNNNGG 1 cut(s) 250
BseRI GAGGAG 2 cut(s) 336, 339
BseXI GCAGC 4 cut(s) 43, 107, 227, 398
Bsh1285I CGRYCG 1 cut(s) 148
BshFI GGCC 3 cut(s) 8, 390, 444
BsiEI CGRYCG 1 cut(s) 148
BsiSI CCGG 3 cut(s) 138, 148, 168
BslFI GGGAC 1 cut(s) 284
BslI CCNNNNNNNGG 1 cut(s) 250
BsmAI GTCTC 1 cut(s) 180
BsmFI GGGAC 1 cut(s) 284
BsnI GGCC 3 cut(s) 8, 390, 444
Bso31I GGTCTC 1 cut(s) 180
Bsp143I GATC 3 cut(s) 38, 331, 397
Bsp19I CCATGG 1 cut(s) 367
BspACI CCGC 4 cut(s) 4, 34, 54, 123
BspANI GGCC 3 cut(s) 8, 390, 444
BspLI GGNNCC 3 cut(s) 40, 328, 351
BspPI GGATC 3 cut(s) 33, 46, 405
BspQI GCTCTTC 1 cut(s) 300
BspTNI GGTCTC 1 cut(s) 180
BsrFI RCCGGY 1 cut(s) 137
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 1 cut(s) 367
BssMI GATC 3 cut(s) 38, 331, 397
BssT1I CCWWGG 1 cut(s) 367
Bst2UI CCWGG 1 cut(s) 387
Bst4CI ACNGT 1 cut(s) 46
Bst6I CTCTTC 3 cut(s) 33, 300, 447
BstAPI GCANNNNNTGC 1 cut(s) 205
BstC8I GCNNGC 1 cut(s) 6
BstDSI CCRYGG 1 cut(s) 367
BstF5I GGATG 2 cut(s) 109, 259
BstHHI GCGC 1 cut(s) 78
BstKTI GATC 3 cut(s) 41, 334, 400
BstMAI GTCTC 1 cut(s) 180
BstMBI GATC 3 cut(s) 38, 331, 397
BstMCI CGRYCG 1 cut(s) 148
BstMWI GCNNNNNNNGC 4 cut(s) 62, 84, 205, 420
BstNI CCWGG 1 cut(s) 387
BstNSI RCATGY 1 cut(s) 101
BstPAI GACNNNNGTC 1 cut(s) 276
BstSCI CCNGG 2 cut(s) 147, 385
BstV1I GCAGC 4 cut(s) 43, 107, 227, 398
BstX2I RGATCY 1 cut(s) 38
BstYI RGATCY 1 cut(s) 38
BsuI GTATCC 1 cut(s) 58
BsuRI GGCC 3 cut(s) 8, 390, 444
BtgI CCRYGG 1 cut(s) 367
BtsCI GGATG 2 cut(s) 109, 259
BtsIMutI CAGTG 1 cut(s) 42
Cac8I GCNNGC 1 cut(s) 6
CfoI GCGC 1 cut(s) 78
Cfr10I RCCGGY 1 cut(s) 137
Cfr13I GGNCC 2 cut(s) 6, 443
Csp6I GTAC 3 cut(s) 233, 259, 343
CviAII CATG 2 cut(s) 98, 368
CviQI GTAC 3 cut(s) 233, 259, 343
DpnI GATC 3 cut(s) 40, 333, 399
DpnII GATC 3 cut(s) 38, 331, 397
DraIII CACNNNGTG 1 cut(s) 113
DrdI GACNNNNNNGTC 1 cut(s) 186
DseDI GACNNNNNNGTC 1 cut(s) 186
Eam1104I CTCTTC 3 cut(s) 33, 300, 447
EarI CTCTTC 3 cut(s) 33, 300, 447
Eco130I CCWWGG 1 cut(s) 367
Eco31I GGTCTC 1 cut(s) 180
Eco57I CTGAAG 1 cut(s) 323
EcoRII CCWGG 1 cut(s) 385
EcoT14I CCWWGG 1 cut(s) 367
ErhI CCWWGG 1 cut(s) 367
FaeI CATG 2 cut(s) 101, 371
FaiI YATR 4 cut(s) 99, 369, 402, 404
FaqI GGGAC 1 cut(s) 284
FatI CATG 2 cut(s) 97, 367
FauI CCCGC 1 cut(s) 27
FauNDI CATATG 1 cut(s) 402
Fnu4HI GCNGC 4 cut(s) 57, 121, 241, 412
FokI GGATG 2 cut(s) 116, 266
Fsp4HI GCNGC 4 cut(s) 57, 121, 241, 412
FspBI CTAG 1 cut(s) 182
GlaI GCGC 1 cut(s) 77
GluI GCNGC 4 cut(s) 57, 121, 241, 412
HaeIII GGCC 3 cut(s) 8, 390, 444
HapII CCGG 3 cut(s) 138, 148, 168
HhaI GCGC 1 cut(s) 78
Hin1II CATG 2 cut(s) 101, 371
Hin6I GCGC 1 cut(s) 76
HinP1I GCGC 1 cut(s) 76
HpaII CCGG 3 cut(s) 138, 148, 168
HphI GGTGA 2 cut(s) 137, 239
Hpy188I TCNGA 3 cut(s) 249, 265, 286
Hpy188III TCNNGA 3 cut(s) 177, 318, 435
Hpy99I CGWCG 1 cut(s) 192
HpyAV CCTTC 1 cut(s) 244
HpyCH4III ACNGT 1 cut(s) 46
HpyCH4IV ACGT 2 cut(s) 112, 231
HpyCH4V TGCA 3 cut(s) 107, 158, 208
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 84, 205, 420
HpySE526I ACGT 2 cut(s) 112, 231
Hsp92II CATG 2 cut(s) 101, 371
HspAI GCGC 1 cut(s) 76
Kzo9I GATC 3 cut(s) 38, 331, 397
LguI GCTCTTC 1 cut(s) 300
LmnI GCTCC 4 cut(s) 64, 326, 428, 458
Lsp1109I GCAGC 4 cut(s) 43, 107, 227, 398
LweI GCATC 3 cut(s) 94, 124, 167
MaeI CTAG 1 cut(s) 182
MaeII ACGT 2 cut(s) 112, 231
MaeIII GTNAC 1 cut(s) 227
MalI GATC 3 cut(s) 40, 333, 399
MboI GATC 3 cut(s) 38, 331, 397
MboII GAAGA 3 cut(s) 20, 317, 464
MflI RGATCY 1 cut(s) 38
MmeI TCCRAC 1 cut(s) 275
MnlI CCTC 5 cut(s) 61, 314, 317, 363, 448
MslI CAYNNNNRTG 1 cut(s) 102
MspA1I CMGCKG 2 cut(s) 56, 120
MspI CCGG 3 cut(s) 138, 148, 168
MspR9I CCNGG 2 cut(s) 149, 387
MvaI CCWGG 1 cut(s) 387
MwoI GCNNNNNNNGC 4 cut(s) 62, 84, 205, 420
NciI CCSGG 1 cut(s) 149
NcoI CCATGG 1 cut(s) 367
NdeI CATATG 1 cut(s) 402
NdeII GATC 3 cut(s) 38, 331, 397
NlaIII CATG 2 cut(s) 101, 371
NlaIV GGNNCC 3 cut(s) 40, 328, 351
NmuCI GTSAC 1 cut(s) 227
NspI RCATGY 1 cut(s) 101
PciSI GCTCTTC 1 cut(s) 300
PcsI WCGNNNNNNNCGW 1 cut(s) 184
PkrI GCNGC 4 cut(s) 58, 122, 242, 413
PshAI GACNNNNGTC 1 cut(s) 276
Psp6I CCWGG 1 cut(s) 385
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 3 cut(s) 40, 328, 351
PspPI GGNCC 2 cut(s) 6, 443
PsuI RGATCY 1 cut(s) 38
PvuII CAGCTG 1 cut(s) 120
RsaI GTAC 3 cut(s) 234, 260, 344
RsaNI GTAC 3 cut(s) 233, 259, 343
RseI CAYNNNNRTG 1 cut(s) 102
SapI GCTCTTC 1 cut(s) 300
SatI GCNGC 4 cut(s) 57, 121, 241, 412
Sau3AI GATC 3 cut(s) 38, 331, 397
Sau96I GGNCC 2 cut(s) 6, 443
ScaI AGTACT 2 cut(s) 260, 344
ScrFI CCNGG 2 cut(s) 149, 387
SetI ASST 8 cut(s) 115, 122, 215, 234, 278, 312, 339, 463
SfaNI GCATC 3 cut(s) 94, 124, 167
SmiMI CAYNNNNRTG 1 cut(s) 102
SpeI ACTAGT 1 cut(s) 181
SsiI CCGC 4 cut(s) 4, 34, 54, 123
SspMI CTAG 1 cut(s) 182
StyD4I CCNGG 2 cut(s) 147, 385
StyI CCWWGG 1 cut(s) 367
TaaI ACNGT 1 cut(s) 46
TaiI ACGT 2 cut(s) 115, 234
TaqI TCGA 1 cut(s) 178
TatI WGTACW 2 cut(s) 258, 342
TscAI CASTG 1 cut(s) 49
TseFI GTSAC 1 cut(s) 227
TseI GCWGC 4 cut(s) 56, 120, 240, 411
Tsp45I GTSAC 1 cut(s) 227
TspGWI ACGGA 1 cut(s) 176
TspRI CASTG 1 cut(s) 49
XceI RCATGY 1 cut(s) 101
XspI CTAG 1 cut(s) 182
ZrmI AGTACT 2 cut(s) 260, 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.