RLG00000025432

F-box kelch-repeat protein At1g57790-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0017680
Physical Location & Seq
Reverse (-)
45745750 .. 45746892
1143 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025432

Sequence Viewer

Length: 975 bp
ATGTCTGTTGTAGTTGCTAATAAGAAACTCAAGAGAGAAATCCCACCACCACAACCACCACCATGGTCTGATCTTAACCTTGACATATTGATTTGCATCATGGGACGCCTTTGTTATGTGGACCAGATTCATTTTCGTGCTCATGATTATTATCCCATAACACTCCATGATCCATCTAGCTGCATTACATATGAGCTTGGTCATGAAATCGATCGGTCAAGTTTGCCGGGGTTTGGCAAACTACAAGTCTGTGGTGCAGGAAAGGACGGTTGGTTGCTTGTAGCAGAGATTTCACCAAACATTTTTACTTTTGACTCTAATATTTACCTCTACAACCCTTTCAACGATAGGGCAGCTGTCAATTTACCCCATTTGACTGGCTCTAGGATTAAAGCCACGTTTGGTGGTACCTCTGATCTAACATCCAAAGATTGTTTGTTTTTTGCGGTGCATGATGTTGGAGAGGATCAAGAGGGAATGAGGATTAGCACTTGCCACCATGGTGATAGAAAGTGGACTAAACAGTTTACAGATCGACGTAGTAGGGAGATAGTAATTGGTGTGTTCCATGTCAAGGGCATCTTCTACTGTGTCTTCAAAAGTGGAATTCTAGCTTTTGAAGCTAGTGTTGTTGAGTCTGATGGAGAGGTGTTGCTAGTGTACTCGAAGCGGGGGTATAAGCCTTGGCACATTTTCAGGCTAGATTGGTTGCAAATGAAATGGGTGAAGGAAGTTAGCTTGGGTAAAAGAGTGTTATTTCTTGGTGACATCTCGTTGTTATATTCTGCGGATGGTGAAATAAAGGATTTAGCTGATCGTATCTATTATCAGGGTTTCAGGAAATCTTACTTCTATCCAATCAAGTCCGGTATTGGTAAGCTCCGCAGCAATGCATTGATACGGCAGTCATGCATACATTATGAGGCACGCAGCGATCATATGCTCCGAGTTTGGATTGAACCACCAACGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

325

Amino Acids

37.1

Weight (kDa)

8.51

Isoelectric Point (pI)

50.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 70 - 276 2.4e-18 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017680)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g05681
prunus_persica Prupe.1G157100_v2.0.a1 Prupe.1G157100_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0454581
rosa_laevigata RLG00000025432
rosa_multiflora Rmu_ssc0000455.1_g000001
rosa_rugosa Rorug03G0000400
rosa_samantha Rh3AG060100 Rh3BG062200 Rh3CG061000 Rh3DG061800
rosa_wichuraiana Rw3G004670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 407
AccB1I GGYRCC 1 cut(s) 407
AciI CCGC 4 cut(s) 446, 670, 788, 883
AclWI GGATC 2 cut(s) 164, 474
AcsI RAATTY 1 cut(s) 606
AcyI GRCGYC 1 cut(s) 106
AfaI GTAC 2 cut(s) 409, 662
AfiI CCNNNNNNNGG 2 cut(s) 233, 574
AgsI TTSAA 4 cut(s) 343, 598, 620, 959
AjuI GAANNNNNNNTTGG 2 cut(s) 253, 285
AleI CACNNNNGTG 1 cut(s) 501
AluBI AGCT 8 cut(s) 180, 196, 356, 614, 623, 738, 812, 880
AluI AGCT 8 cut(s) 180, 196, 356, 614, 623, 738, 812, 880
Alw21I GWGCWC 1 cut(s) 142
AlwI GGATC 2 cut(s) 164, 474
ApeKI GCWGC 4 cut(s) 180, 353, 885, 930
ApoI RAATTY 1 cut(s) 606
ArsI GACNNNNNNTTYG 2 cut(s) 231, 263
Asp718I GGTACC 1 cut(s) 407
AspS9I GGNCC 1 cut(s) 121
AsuC2I CCSGG 1 cut(s) 228
AsuHPI GGTGA 5 cut(s) 285, 515, 736, 776, 806
AvaII GGWCC 1 cut(s) 121
BaeI ACNNNNGTAYC 2 cut(s) 890, 923
BanI GGYRCC 1 cut(s) 407
BbsI GAAGAC 1 cut(s) 586
Bbv12I GWGCWC 1 cut(s) 142
BbvI GCAGC 4 cut(s) 167, 365, 897, 942
BccI CCATC 3 cut(s) 181, 635, 785
BceAI ACGGC 1 cut(s) 917
BcnI CCSGG 1 cut(s) 228
BfaI CTAG 6 cut(s) 177, 384, 611, 624, 656, 701
BisI GCNGC 4 cut(s) 181, 354, 886, 931
BlsI GCNGC 4 cut(s) 182, 355, 887, 932
Bme1390I CCNGG 1 cut(s) 228
Bme18I GGWCC 1 cut(s) 121
BmgT120I GGNCC 1 cut(s) 121
BmiI GGNNCC 1 cut(s) 409
BmrFI CCNGG 1 cut(s) 228
BmsI GCATC 2 cut(s) 105, 588
BpiI GAAGAC 1 cut(s) 586
BpuEI CTTGAG 1 cut(s) 14
BpuMI CCSGG 1 cut(s) 228
Bsa29I ATCGAT 1 cut(s) 210
BsaBI GATNNNNATC 2 cut(s) 95, 150
BsaHI GRCGYC 1 cut(s) 106
BsaJI CCNNGG 4 cut(s) 62, 227, 499, 683
BsaWI WCCGGW 1 cut(s) 866
Bsc4I CCNNNNNNNGG 2 cut(s) 233, 574
Bse1I ACTGG 1 cut(s) 382
Bse3DI GCAATG 1 cut(s) 895
Bse8I GATNNNNATC 2 cut(s) 95, 150
BseCI ATCGAT 1 cut(s) 210
BseDI CCNNGG 4 cut(s) 62, 227, 499, 683
BseGI GGATG 2 cut(s) 422, 796
BseJI GATNNNNATC 2 cut(s) 95, 150
BseLI CCNNNNNNNGG 2 cut(s) 233, 574
BseMI GCAATG 1 cut(s) 895
BseNI ACTGG 1 cut(s) 382
BseXI GCAGC 4 cut(s) 167, 365, 897, 942
BsgI GTGCAG 1 cut(s) 276
Bsh1285I CGRYCG 1 cut(s) 214
BshNI GGYRCC 1 cut(s) 407
BshVI ATCGAT 1 cut(s) 210
BsiEI CGRYCG 1 cut(s) 214
BsiHKAI GWGCWC 1 cut(s) 142
BsiSI CCGG 2 cut(s) 227, 867
BslFI GGGAC 1 cut(s) 117
BslI CCNNNNNNNGG 2 cut(s) 233, 574
BsmFI GGGAC 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 142
Bsp143I GATC 8 cut(s) 70, 169, 211, 415, 466, 532, 814, 934
Bsp19I CCATGG 2 cut(s) 62, 499
BspACI CCGC 4 cut(s) 446, 670, 788, 883
BspDI ATCGAT 1 cut(s) 210
BspHI TCATGA 2 cut(s) 142, 202
BspLI GGNNCC 1 cut(s) 409
BspPI GGATC 2 cut(s) 164, 474
BspT107I GGYRCC 1 cut(s) 407
BsrDI GCAATG 1 cut(s) 895
BsrI ACTGG 1 cut(s) 382
BssECI CCNNGG 4 cut(s) 62, 227, 499, 683
BssMI GATC 8 cut(s) 70, 169, 211, 415, 466, 532, 814, 934
BssNI GRCGYC 1 cut(s) 106
BssT1I CCWWGG 3 cut(s) 62, 499, 683
Bst4CI ACNGT 3 cut(s) 269, 525, 590
BstACI GRCGYC 1 cut(s) 106
BstC8I GCNNGC 1 cut(s) 928
BstDSI CCRYGG 2 cut(s) 62, 499
BstF5I GGATG 2 cut(s) 422, 796
BstKTI GATC 8 cut(s) 73, 172, 214, 418, 469, 535, 817, 937
BstMBI GATC 8 cut(s) 70, 169, 211, 415, 466, 532, 814, 934
BstMCI CGRYCG 1 cut(s) 214
BstMWI GCNNNNNNNGC 1 cut(s) 620
BstSCI CCNGG 1 cut(s) 226
BstV1I GCAGC 4 cut(s) 167, 365, 897, 942
BstV2I GAAGAC 1 cut(s) 586
BstXI CCANNNNNNTGG 2 cut(s) 63, 377
Bsu15I ATCGAT 1 cut(s) 210
BsuTUI ATCGAT 1 cut(s) 210
BtgI CCRYGG 2 cut(s) 62, 499
BtsCI GGATG 2 cut(s) 422, 796
Cac8I GCNNGC 1 cut(s) 928
CciI TCATGA 2 cut(s) 142, 202
Cfr13I GGNCC 1 cut(s) 121
ClaI ATCGAT 1 cut(s) 210
CseI GACGC 1 cut(s) 114
Csp6I GTAC 2 cut(s) 408, 661
CviAII CATG 9 cut(s) 63, 100, 143, 167, 203, 452, 500, 569, 909
CviQI GTAC 2 cut(s) 408, 661
DpnI GATC 8 cut(s) 72, 171, 213, 417, 468, 534, 816, 936
DpnII GATC 8 cut(s) 70, 169, 211, 415, 466, 532, 814, 934
Eco130I CCWWGG 3 cut(s) 62, 499, 683
Eco47I GGWCC 1 cut(s) 121
EcoRI GAATTC 1 cut(s) 606
EcoT14I CCWWGG 3 cut(s) 62, 499, 683
EcoT22I ATGCAT 2 cut(s) 895, 914
ErhI CCWWGG 3 cut(s) 62, 499, 683
FaeI CATG 9 cut(s) 66, 103, 146, 170, 206, 455, 503, 572, 912
FalI AAGNNNNNCTT 2 cut(s) 566, 598
FaqI GGGAC 1 cut(s) 117
FatI CATG 9 cut(s) 62, 99, 142, 166, 202, 451, 499, 568, 908
FauI CCCGC 1 cut(s) 663
FauNDI CATATG 2 cut(s) 190, 939
Fnu4HI GCNGC 4 cut(s) 181, 354, 886, 931
FokI GGATG 2 cut(s) 409, 803
Fsp4HI GCNGC 4 cut(s) 181, 354, 886, 931
FspBI CTAG 6 cut(s) 177, 384, 611, 624, 656, 701
GluI GCNGC 4 cut(s) 181, 354, 886, 931
HapII CCGG 2 cut(s) 227, 867
HgaI GACGC 1 cut(s) 114
Hin1I GRCGYC 1 cut(s) 106
Hin1II CATG 9 cut(s) 66, 103, 146, 170, 206, 455, 503, 572, 912
HinfI GANTC 3 cut(s) 127, 314, 635
HpaII CCGG 2 cut(s) 227, 867
HphI GGTGA 5 cut(s) 285, 515, 736, 776, 806
Hpy166II GTNNAC 4 cut(s) 121, 516, 528, 661
Hpy188I TCNGA 4 cut(s) 70, 415, 640, 947
Hpy188III TCNNGA 5 cut(s) 31, 143, 203, 470, 838
Hpy8I GTNNAC 4 cut(s) 121, 516, 528, 661
Hpy99I CGWCG 1 cut(s) 540
HpyAV CCTTC 1 cut(s) 721
HpyCH4III ACNGT 3 cut(s) 269, 525, 590
HpyCH4IV ACGT 2 cut(s) 398, 538
HpyCH4V TGCA 7 cut(s) 96, 183, 257, 451, 712, 893, 912
HpyF10VI GCNNNNNNNGC 1 cut(s) 620
HpySE526I ACGT 2 cut(s) 398, 538
Hsp92I GRCGYC 1 cut(s) 106
Hsp92II CATG 9 cut(s) 66, 103, 146, 170, 206, 455, 503, 572, 912
KpnI GGTACC 1 cut(s) 411
Kzo9I GATC 8 cut(s) 70, 169, 211, 415, 466, 532, 814, 934
LmnI GCTCC 2 cut(s) 885, 948
LpnPI CCDG 8 cut(s) 137, 240, 243, 363, 682, 815, 823, 880
Lsp1109I GCAGC 4 cut(s) 167, 365, 897, 942
LweI GCATC 2 cut(s) 105, 588
MaeI CTAG 6 cut(s) 177, 384, 611, 624, 656, 701
MaeII ACGT 2 cut(s) 398, 538
MaeIII GTNAC 1 cut(s) 764
MalI GATC 8 cut(s) 72, 171, 213, 417, 468, 534, 816, 936
MboI GATC 8 cut(s) 70, 169, 211, 415, 466, 532, 814, 934
MboII GAAGA 2 cut(s) 574, 586
MhlI GDGCHC 1 cut(s) 142
MluCI AATT 3 cut(s) 361, 555, 606
MlyI GAGTC 2 cut(s) 308, 644
MmeI TCCRAC 1 cut(s) 439
MnlI CCTC 7 cut(s) 338, 421, 457, 466, 474, 640, 916
Mph1103I ATGCAT 2 cut(s) 895, 914
MseI TTAA 2 cut(s) 75, 390
MslI CAYNNNNRTG 3 cut(s) 61, 135, 501
MspA1I CMGCKG 1 cut(s) 356
MspI CCGG 2 cut(s) 227, 867
MspR9I CCNGG 1 cut(s) 228
MwoI GCNNNNNNNGC 1 cut(s) 620
NciI CCSGG 1 cut(s) 228
NcoI CCATGG 2 cut(s) 62, 499
NdeI CATATG 2 cut(s) 190, 939
NdeII GATC 8 cut(s) 70, 169, 211, 415, 466, 532, 814, 934
NlaIII CATG 9 cut(s) 66, 103, 146, 170, 206, 455, 503, 572, 912
NlaIV GGNNCC 1 cut(s) 409
NmuCI GTSAC 1 cut(s) 764
NsiI ATGCAT 2 cut(s) 895, 914
OliI CACNNNNGTG 1 cut(s) 501
PagI TCATGA 2 cut(s) 142, 202
PfeI GAWTC 1 cut(s) 127
PkrI GCNGC 4 cut(s) 182, 355, 887, 932
Ple19I CGATCG 1 cut(s) 214
PleI GAGTC 2 cut(s) 308, 643
PpsI GAGTC 2 cut(s) 308, 643
PspN4I GGNNCC 1 cut(s) 409
PspPI GGNCC 1 cut(s) 121
PvuI CGATCG 1 cut(s) 214
PvuII CAGCTG 1 cut(s) 356
RsaI GTAC 2 cut(s) 409, 662
RsaNI GTAC 2 cut(s) 408, 661
RseI CAYNNNNRTG 3 cut(s) 61, 135, 501
SaqAI TTAA 2 cut(s) 75, 390
SatI GCNGC 4 cut(s) 181, 354, 886, 931
Sau3AI GATC 8 cut(s) 70, 169, 211, 415, 466, 532, 814, 934
Sau96I GGNCC 1 cut(s) 121
SchI GAGTC 2 cut(s) 308, 644
ScrFI CCNGG 1 cut(s) 228
SduI GDGCHC 1 cut(s) 142
SfaNI GCATC 2 cut(s) 105, 588
SinI GGWCC 1 cut(s) 121
SmiMI CAYNNNNRTG 3 cut(s) 61, 135, 501
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 3 cut(s) 361, 555, 606
SsiI CCGC 4 cut(s) 446, 670, 788, 883
SspI AATATT 1 cut(s) 322
SspMI CTAG 6 cut(s) 177, 384, 611, 624, 656, 701
StyD4I CCNGG 1 cut(s) 226
StyI CCWWGG 3 cut(s) 62, 499, 683
TaaI ACNGT 3 cut(s) 269, 525, 590
TaiI ACGT 2 cut(s) 401, 541
TaqI TCGA 3 cut(s) 210, 535, 665
TaqII GACCGA 1 cut(s) 204
TasI AATT 3 cut(s) 361, 555, 606
TatI WGTACW 1 cut(s) 660
TfiI GAWTC 1 cut(s) 127
Tru1I TTAA 2 cut(s) 75, 390
Tru9I TTAA 2 cut(s) 75, 390
TseFI GTSAC 1 cut(s) 764
TseI GCWGC 4 cut(s) 180, 353, 885, 930
Tsp45I GTSAC 1 cut(s) 764
TspDTI ATGAA 3 cut(s) 119, 219, 731
VpaK11BI GGWCC 1 cut(s) 121
XapI RAATTY 1 cut(s) 606
XspI CTAG 6 cut(s) 177, 384, 611, 624, 656, 701
Zsp2I ATGCAT 2 cut(s) 895, 914
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.