Rh3DG061800

F-box kelch-repeat protein At1g57790-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0017680
Physical Location & Seq
Forward (+)
4143004 .. 4145111
2108 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG061800.1

Sequence Viewer

Length: 1170 bp
ATGTCAGTTGTAGTTGCCAATAAGAAACTCAAGAGAGAAATCCCACCACCACAACCACCACCATGGTCTGATCTTAACCTTGACATATTGATTTGCATCATGGGACGCCTTTGTTATGTGGACCAGATTCATTTTCGTGCTGTTTGCAAGAATTGGAGACTAGTTTCTGGTGTGAAGCCTATAGATAAACTCCCTTGGGTTCTGACATTGAATTTCAATTGCAAGCATGATTATTATCCCATAACCCTCCATGATCCATCTAGTTGCATTACATATGAGCTTGGTCATGAAATCGATCGGTCCAGTTTGCCTGGGTTTGGCAAACTACAAGTCTGTGGTGCAGGAAAGAACGGTTGGTTGCTTGTAGCAGAGATTTCACCTAACATTTCTTTTGACTCTAATATTTACCTCTACAATCCTTTCAACGATTGGGCCGCTATCAAATTACCCCATTTGACTAGCTCTAGGATTAAAGCCACGTTTGGTGGTACCTCTGATCTAACATCCGAAGATTGTTTGTTTTTCGCGGTGCATGATGTTGGAGAGGATCAAGAGGGAATGAGGATTAGCACTTGCCGCCATGGTGATAGAAAGTGGACTAAACAGTTTACAGATCGACGTAGTAGGGAGATAGTAATTGGTGTGTTCCATGTCAAGGGCATCTTCTTCTGTGTCTTCAAAAGTGGAATTCTAGGTTCATTCAACCCGGATACCAAAATTTGGAGCTTGCTGATTACTTCGGAAAACATGGATTTAGTGGAGTTACGAAACCTAGCAGCTTTTGAAGCTAGTGTTGTTGAGTCTGATGGAGAGGTGTTGCTAGTGTACTGGAAGCGGGGGTATAAGCCTTGGCACATTTTCAGGCTAGATTGGTTGCAAATGAAATGGGTGAAGGAGGTTAGCTTGGGTAACAGAGTGTTATTTCTTGGTGACATCTCGTTGTTGTATTCTGCTGATGGTGAAATAAAGGATTTAGCTGATCGTATCTATTATCAGGGTTTCAGGAAATCTTACTTCTATCCAATCAAGTCCGGTATTGGTAAGCTCCGCAGCAATGCATTGATACGCCAGTCATGCATACATTATGAGGCACGCAGCGATCATATGCTCAGAGTTTGGATTGAACCACCAACGCAATACAGAGATGCAGTACATGGCAGCATGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

389

Amino Acids

44.68

Weight (kDa)

8.56

Isoelectric Point (pI)

40.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 22 - 54 3.8e-07 F-box domain
Beta-prop_KIB1-4 PF03478 98 - 331 1.7e-21 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017680)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g05681
prunus_persica Prupe.1G157100_v2.0.a1 Prupe.1G157100_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0454581
rosa_laevigata RLG00000025432
rosa_multiflora Rmu_ssc0000455.1_g000001
rosa_rugosa Rorug03G0000400
rosa_samantha Rh3AG060100 Rh3BG062200 Rh3CG061000 Rh3DG061800
rosa_wichuraiana Rw3G004670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 488
AccB1I GGYRCC 1 cut(s) 488
AccB7I CCANNNNNTGG 1 cut(s) 720
AccII CGCG 1 cut(s) 527
AciI CCGC 5 cut(s) 435, 527, 577, 835, 1048
AclWI GGATC 2 cut(s) 248, 555
AcsI RAATTY 3 cut(s) 211, 687, 717
AcyI GRCGYC 1 cut(s) 106
AfaI GTAC 3 cut(s) 490, 827, 1152
AfiI CCNNNNNNNGG 3 cut(s) 317, 655, 720
AgsI TTSAA 7 cut(s) 211, 217, 424, 679, 703, 785, 1124
AhlI ACTAGT 1 cut(s) 160
AjnI CCWGG 1 cut(s) 310
AjuI GAANNNNNNNTTGG 2 cut(s) 337, 369
AluBI AGCT 8 cut(s) 280, 462, 726, 779, 788, 903, 977, 1045
AluI AGCT 8 cut(s) 280, 462, 726, 779, 788, 903, 977, 1045
Alw26I GTCTC 1 cut(s) 151
AlwI GGATC 2 cut(s) 248, 555
AoxI GGCC 1 cut(s) 432
ApeKI GCWGC 4 cut(s) 776, 1050, 1095, 1158
ApoI RAATTY 3 cut(s) 211, 687, 717
ArsI GACNNNNNNTTYG 2 cut(s) 315, 347
Asp718I GGTACC 1 cut(s) 488
AspS9I GGNCC 3 cut(s) 121, 300, 432
AsuC2I CCSGG 1 cut(s) 707
AsuHPI GGTGA 5 cut(s) 369, 596, 901, 941, 971
AvaII GGWCC 2 cut(s) 121, 300
BaeI ACNNNNGTAYC 2 cut(s) 1055, 1088
BanI GGYRCC 1 cut(s) 488
BbsI GAAGAC 1 cut(s) 667
BbvI GCAGC 3 cut(s) 788, 1062, 1107
BccI CCATC 3 cut(s) 265, 800, 950
BciT130I CCWGG 1 cut(s) 312
BciVI GTATCC 1 cut(s) 703
BcnI CCSGG 1 cut(s) 707
BcoDI GTCTC 1 cut(s) 151
BcuI ACTAGT 1 cut(s) 160
BfaI CTAG 9 cut(s) 161, 261, 459, 465, 692, 773, 789, 821, 866
BfmI CTRYAG 1 cut(s) 180
BfuI GTATCC 1 cut(s) 703
BisI GCNGC 6 cut(s) 435, 577, 777, 1051, 1096, 1159
BlsI GCNGC 6 cut(s) 436, 578, 778, 1052, 1097, 1160
Bme1390I CCNGG 2 cut(s) 312, 707
Bme18I GGWCC 2 cut(s) 121, 300
BmgT120I GGNCC 3 cut(s) 121, 300, 432
BmiI GGNNCC 1 cut(s) 490
BmrFI CCNGG 2 cut(s) 312, 707
BmsI GCATC 3 cut(s) 105, 669, 1135
BpiI GAAGAC 1 cut(s) 667
BpuEI CTTGAG 1 cut(s) 14
BpuMI CCSGG 1 cut(s) 707
Bsa29I ATCGAT 1 cut(s) 294
BsaBI GATNNNNATC 2 cut(s) 95, 234
BsaHI GRCGYC 1 cut(s) 106
BsaJI CCNNGG 5 cut(s) 62, 194, 311, 580, 848
BsaWI WCCGGW 1 cut(s) 1031
Bsc4I CCNNNNNNNGG 3 cut(s) 317, 655, 720
Bse1I ACTGG 3 cut(s) 303, 833, 1069
Bse3DI GCAATG 1 cut(s) 1060
Bse8I GATNNNNATC 2 cut(s) 95, 234
BseBI CCWGG 1 cut(s) 312
BseCI ATCGAT 1 cut(s) 294
BseDI CCNNGG 5 cut(s) 62, 194, 311, 580, 848
BseGI GGATG 1 cut(s) 503
BseJI GATNNNNATC 2 cut(s) 95, 234
BseLI CCNNNNNNNGG 3 cut(s) 317, 655, 720
BseMI GCAATG 1 cut(s) 1060
BseMII CTCAG 1 cut(s) 1123
BseNI ACTGG 3 cut(s) 303, 833, 1069
BseXI GCAGC 3 cut(s) 788, 1062, 1107
BsgI GTGCAG 1 cut(s) 360
Bsh1236I CGCG 1 cut(s) 527
Bsh1285I CGRYCG 1 cut(s) 298
BshFI GGCC 1 cut(s) 434
BshNI GGYRCC 1 cut(s) 488
BshVI ATCGAT 1 cut(s) 294
BsiEI CGRYCG 1 cut(s) 298
BsiSI CCGG 2 cut(s) 707, 1032
BslFI GGGAC 1 cut(s) 117
BslI CCNNNNNNNGG 3 cut(s) 317, 655, 720
BsmAI GTCTC 1 cut(s) 151
BsmFI GGGAC 1 cut(s) 117
BsnI GGCC 1 cut(s) 434
Bsp143I GATC 8 cut(s) 70, 253, 295, 496, 547, 613, 979, 1099
Bsp19I CCATGG 2 cut(s) 62, 580
BspACI CCGC 5 cut(s) 435, 527, 577, 835, 1048
BspANI GGCC 1 cut(s) 434
BspCNI CTCAG 1 cut(s) 1122
BspDI ATCGAT 1 cut(s) 294
BspFNI CGCG 1 cut(s) 527
BspHI TCATGA 1 cut(s) 286
BspLI GGNNCC 1 cut(s) 490
BspPI GGATC 2 cut(s) 248, 555
BspT107I GGYRCC 1 cut(s) 488
BsrDI GCAATG 1 cut(s) 1060
BsrI ACTGG 3 cut(s) 303, 833, 1069
BssECI CCNNGG 5 cut(s) 62, 194, 311, 580, 848
BssMI GATC 8 cut(s) 70, 253, 295, 496, 547, 613, 979, 1099
BssNI GRCGYC 1 cut(s) 106
BssT1I CCWWGG 4 cut(s) 62, 194, 580, 848
Bst2UI CCWGG 1 cut(s) 312
Bst4CI ACNGT 2 cut(s) 353, 606
BstACI GRCGYC 1 cut(s) 106
BstC8I GCNNGC 3 cut(s) 224, 728, 1093
BstDEI CTNAG 1 cut(s) 1109
BstDSI CCRYGG 2 cut(s) 62, 580
BstF5I GGATG 1 cut(s) 503
BstFNI CGCG 1 cut(s) 527
BstKTI GATC 8 cut(s) 73, 256, 298, 499, 550, 616, 982, 1102
BstMAI GTCTC 1 cut(s) 151
BstMBI GATC 8 cut(s) 70, 253, 295, 496, 547, 613, 979, 1099
BstMCI CGRYCG 1 cut(s) 298
BstMWI GCNNNNNNNGC 3 cut(s) 576, 785, 1074
BstNI CCWGG 1 cut(s) 312
BstSCI CCNGG 2 cut(s) 310, 705
BstSFI CTRYAG 1 cut(s) 180
BstUI CGCG 1 cut(s) 527
BstV1I GCAGC 3 cut(s) 788, 1062, 1107
BstV2I GAAGAC 1 cut(s) 667
BstXI CCANNNNNNTGG 1 cut(s) 63
Bsu15I ATCGAT 1 cut(s) 294
BsuI GTATCC 1 cut(s) 703
BsuRI GGCC 1 cut(s) 434
BsuTUI ATCGAT 1 cut(s) 294
BtgI CCRYGG 2 cut(s) 62, 580
BtsCI GGATG 1 cut(s) 503
Cac8I GCNNGC 3 cut(s) 224, 728, 1093
CciI TCATGA 1 cut(s) 286
Cfr13I GGNCC 3 cut(s) 121, 300, 432
ClaI ATCGAT 1 cut(s) 294
CseI GACGC 1 cut(s) 114
Csp6I GTAC 3 cut(s) 489, 826, 1151
CviQI GTAC 3 cut(s) 489, 826, 1151
DdeI CTNAG 1 cut(s) 1109
DpnI GATC 8 cut(s) 72, 255, 297, 498, 549, 615, 981, 1101
DpnII GATC 8 cut(s) 70, 253, 295, 496, 547, 613, 979, 1099
Eco130I CCWWGG 4 cut(s) 62, 194, 580, 848
Eco47I GGWCC 2 cut(s) 121, 300
EcoRI GAATTC 1 cut(s) 687
EcoRII CCWGG 1 cut(s) 310
EcoT14I CCWWGG 4 cut(s) 62, 194, 580, 848
EcoT22I ATGCAT 2 cut(s) 1060, 1079
ErhI CCWWGG 4 cut(s) 62, 194, 580, 848
FalI AAGNNNNNCTT 2 cut(s) 647, 679
FaqI GGGAC 1 cut(s) 117
FauI CCCGC 1 cut(s) 828
FauNDI CATATG 2 cut(s) 274, 1104
Fnu4HI GCNGC 6 cut(s) 435, 577, 777, 1051, 1096, 1159
FokI GGATG 1 cut(s) 490
Fsp4HI GCNGC 6 cut(s) 435, 577, 777, 1051, 1096, 1159
FspBI CTAG 9 cut(s) 161, 261, 459, 465, 692, 773, 789, 821, 866
GluI GCNGC 6 cut(s) 435, 577, 777, 1051, 1096, 1159
HaeIII GGCC 1 cut(s) 434
HapII CCGG 2 cut(s) 707, 1032
HgaI GACGC 1 cut(s) 114
Hin1I GRCGYC 1 cut(s) 106
HinfI GANTC 3 cut(s) 127, 395, 800
HpaII CCGG 2 cut(s) 707, 1032
HphI GGTGA 5 cut(s) 369, 596, 901, 941, 971
Hpy166II GTNNAC 4 cut(s) 121, 597, 609, 826
Hpy188I TCNGA 7 cut(s) 70, 204, 496, 508, 742, 805, 1112
Hpy188III TCNNGA 4 cut(s) 31, 287, 551, 1003
Hpy8I GTNNAC 4 cut(s) 121, 597, 609, 826
Hpy99I CGWCG 1 cut(s) 621
HpyAV CCTTC 1 cut(s) 886
HpyCH4III ACNGT 2 cut(s) 353, 606
HpyCH4IV ACGT 2 cut(s) 479, 619
HpyF10VI GCNNNNNNNGC 3 cut(s) 576, 785, 1074
HpyF3I CTNAG 1 cut(s) 1109
HpySE526I ACGT 2 cut(s) 479, 619
Hsp92I GRCGYC 1 cut(s) 106
KpnI GGTACC 1 cut(s) 492
Kzo9I GATC 8 cut(s) 70, 253, 295, 496, 547, 613, 979, 1099
LmnI GCTCC 2 cut(s) 723, 1050
Lsp1109I GCAGC 3 cut(s) 788, 1062, 1107
LweI GCATC 3 cut(s) 105, 669, 1135
MaeI CTAG 9 cut(s) 161, 261, 459, 465, 692, 773, 789, 821, 866
MaeII ACGT 2 cut(s) 479, 619
MaeIII GTNAC 3 cut(s) 762, 908, 929
MalI GATC 8 cut(s) 72, 255, 297, 498, 549, 615, 981, 1101
MboI GATC 8 cut(s) 70, 253, 295, 496, 547, 613, 979, 1099
MboII GAAGA 4 cut(s) 521, 655, 658, 667
MfeI CAATTG 1 cut(s) 217
MluCI AATT 8 cut(s) 151, 211, 217, 443, 636, 687, 717, 1165
MlyI GAGTC 2 cut(s) 389, 809
MmeI TCCRAC 1 cut(s) 520
MnlI CCTC 9 cut(s) 257, 419, 502, 538, 547, 555, 805, 889, 1081
Mph1103I ATGCAT 2 cut(s) 1060, 1079
MseI TTAA 2 cut(s) 75, 471
MslI CAYNNNNRTG 2 cut(s) 61, 135
MspI CCGG 2 cut(s) 707, 1032
MspR9I CCNGG 2 cut(s) 312, 707
MunI CAATTG 1 cut(s) 217
MvaI CCWGG 1 cut(s) 312
MvnI CGCG 1 cut(s) 527
MwoI GCNNNNNNNGC 3 cut(s) 576, 785, 1074
NciI CCSGG 1 cut(s) 707
NcoI CCATGG 2 cut(s) 62, 580
NdeI CATATG 2 cut(s) 274, 1104
NdeII GATC 8 cut(s) 70, 253, 295, 496, 547, 613, 979, 1099
NlaIV GGNNCC 1 cut(s) 490
NmuCI GTSAC 1 cut(s) 929
NsiI ATGCAT 2 cut(s) 1060, 1079
PagI TCATGA 1 cut(s) 286
PfeI GAWTC 1 cut(s) 127
PflMI CCANNNNNTGG 1 cut(s) 720
PkrI GCNGC 6 cut(s) 436, 578, 778, 1052, 1097, 1160
Ple19I CGATCG 1 cut(s) 298
PleI GAGTC 2 cut(s) 389, 808
PpsI GAGTC 2 cut(s) 389, 808
Psp6I CCWGG 1 cut(s) 310
PspGI CCWGG 1 cut(s) 310
PspN4I GGNNCC 1 cut(s) 490
PspPI GGNCC 3 cut(s) 121, 300, 432
PvuI CGATCG 1 cut(s) 298
RsaI GTAC 3 cut(s) 490, 827, 1152
RsaNI GTAC 3 cut(s) 489, 826, 1151
RseI CAYNNNNRTG 2 cut(s) 61, 135
SaqAI TTAA 2 cut(s) 75, 471
SatI GCNGC 6 cut(s) 435, 577, 777, 1051, 1096, 1159
Sau3AI GATC 8 cut(s) 70, 253, 295, 496, 547, 613, 979, 1099
Sau96I GGNCC 3 cut(s) 121, 300, 432
SchI GAGTC 2 cut(s) 389, 809
ScrFI CCNGG 2 cut(s) 312, 707
SfaNI GCATC 3 cut(s) 105, 669, 1135
SfcI CTRYAG 1 cut(s) 180
SinI GGWCC 2 cut(s) 121, 300
SmiMI CAYNNNNRTG 2 cut(s) 61, 135
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
SpeI ACTAGT 1 cut(s) 160
Sse9I AATT 8 cut(s) 151, 211, 217, 443, 636, 687, 717, 1165
SsiI CCGC 5 cut(s) 435, 527, 577, 835, 1048
SspI AATATT 1 cut(s) 403
SspMI CTAG 9 cut(s) 161, 261, 459, 465, 692, 773, 789, 821, 866
StyD4I CCNGG 2 cut(s) 310, 705
StyI CCWWGG 4 cut(s) 62, 194, 580, 848
TaaI ACNGT 2 cut(s) 353, 606
TaiI ACGT 2 cut(s) 482, 622
TaqI TCGA 2 cut(s) 294, 616
TaqII GACCGA 1 cut(s) 288
TasI AATT 8 cut(s) 151, 211, 217, 443, 636, 687, 717, 1165
TatI WGTACW 2 cut(s) 825, 1150
TauI GCSGC 2 cut(s) 437, 579
TfiI GAWTC 1 cut(s) 127
Tru1I TTAA 2 cut(s) 75, 471
Tru9I TTAA 2 cut(s) 75, 471
TseFI GTSAC 1 cut(s) 929
TseI GCWGC 4 cut(s) 776, 1050, 1095, 1158
Tsp45I GTSAC 1 cut(s) 929
TspDTI ATGAA 4 cut(s) 119, 303, 687, 896
Van91I CCANNNNNTGG 1 cut(s) 720
VpaK11BI GGWCC 2 cut(s) 121, 300
XapI RAATTY 3 cut(s) 211, 687, 717
XspI CTAG 9 cut(s) 161, 261, 459, 465, 692, 773, 789, 821, 866
Zsp2I ATGCAT 2 cut(s) 1060, 1079
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.