Rorug03G0000400

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001934
Physical Location & Seq
Reverse (-)
67903 .. 78184
10282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0000400.1

Sequence Viewer

Length: 3369 bp
ATGGAAGAAAACGACGGTTTCTTATCGTCTCTGAGCCTCCGTAACTCCTCCGCGCCAATAACAAGACCAAACCAAAACGATGGTCTCGACGGCGGCAAACTCTTCGTGGACTCTTCACCATCTTCATCGGAAGATGAAAATGACGCCGTCTCCCTCCACTCCACTTCAAAACGCCTCGACTATATGCTCCAATTCCTCGACCGCAAGCTCTCCGTTGCCAACGTCAACGGCAACGTCAACGTCAACGTCGTCGACCACATCAACAATATCAATATGAGCGACGCCGCCGACACTGCTCAGGGCTCCTCCTCGCTCCCTGAGTTCGAGGCCAAGGGTGGAGGTACCGGAATGTTCAAAGTCCCGGTTCGGGCGGCGGTCCACCCGAACCGGCCGCCAAGGCTGGAACTGAGGCCCCAACCGCTGAGGGAGACCCAGATAGGATGCTTCTTGCGGACTGTGGTCAGCACCGAGTCCCAATTGTGGGCTGGGACGGAGTGCGCCGTCAGGGTTTGGAATTTTAAAGATCTGTACTCGCCGGCGCCGGCTGAGGCTGGACGAGGGGAAGGCGATTCGGAAACGGCACCGTTTAGGGAGTCCGTGTGCACATCCGCGGTGATGTGTTTAGTTGGGGATGATGGGAATAGGGTGGTGTGGAGTGGACATAGGGATGGTAAAATTAGGTGTTGGAGTATGGACTCCTCCTCCTCCTCCTCAGCACTAGTAACTGCTCCTTTTAAGGAGGGCTTGTCTTGGCAGGCTCATAGAGGCCCCGTTCTTTCACTTGTTATTTCTTGTTACGGGGATCTCTGGTCAGGTTCAGAGGGTGGTGTCATCAAGATATGGCCATGGGAAGCTATTGAAAAAGCTCTGTCTCTTACCATTGAAGAAAGGCACATGTCTTCTTTGTTGGTGGAGAGGTCTTACATTGATCCCTGGACCCAAGTTGCCGTCAATGGTTTCACTAATGTATTAACTTCAGATGTTAGGTACTTATTATCTGATAGCTCCAGTGCAAAGGTGTGGAGTGCTGGTTATCTCTCATTCGCACTGTGGGATGCGCGCACAAGAGAATTACTGAAAGTGTTCAATACCGATGGTCTGAATGAGAATCGAGTGGACATTTCATCAGCACAGGACTTGTCAGTGGAATTTATTTCTGGACCAAAAAAAGACAAAACACAAAGTTCTTTTGGTTTCTTTCAGCGATCACGTAATGCTTTAATGGGAGCAGCAGATGCTGTTCGTCGAGTTGCAGTGAAGGGTGCCTTTGGAGATGATAATCGAAGAACTGAAGCATTGGTAATTGCAGTAGATACAATGATTTGGACGGGGTGTACAAATGGCCTACTTGTGCAGTGGGATAGAAATGGCAACCGCATGCAAGAATTCCATTACCATTCTTCTGCTGTCCAATGCTTTTGTACTTTTGGGCTACGTATATGGGTGGGTTATGCAAGTGGCACTGTCCAGGTATTGGACCTCGATGGTAATCTGCTTGGAGGATGGGTAGCTGACAACAGTCCTATTATTAAAATAGCTACAGGAGCTGGTTATGTATTTACCTTGGCCAATCATGGTGGTATATGTGGATGGAATATTACATCACCGGGACCTCTTGACAGCATAGTGCGTTCAGAATTAGCTGGCAAAGAATTTCTATATACAAGGATAGAAAACTTGAAAATATTGACTGGTACATGGAATGTTGGACAAGGACGAGCGTCTCAGGACTCACTTATATCCTGGCTAGGCTCTGTGGCTTCTGATGTTGGCATTGTTGTTGTTGGTTTGCAAGAAGTTGAAATGGGTGCTGGTTTTCTTGCTATGTCTGCAGCCAAAGAAACTGTAGGTCTTGAGGGGAGCTCTGTTGGGCAGTGGTGGCTGGATATGATTGGAAAAACATTGGATGAAGGGTCAACCTTTGAACGTGTTGGATCCAGACAGTTGGCAGGATTGCTCATTGCTATGTGGGTGAGACATAATCTCAAAGCTCATGTCGGAGATGTCGACGCTGCAGCAGTTCCGTGTGGTTTTGGGCGCGCTATTGGTAACAAGGGAGCTGTTGGTTTGAGGATTAGATTGTATGACAGGATAATGTGCTTTGTTAATTGTCACTTTGCTGCGCATTTGGAAGCTGTTAATCGCCGCAACGCAGATTTCGATCATGTATATCGAACCATGACCTTCTCCCGACCTAATTTTCTCAATTGTGCAGCTGCTAGTGCTTCATCTGCTGTTCAAATGCTTCGCGGTACAAATGCTTTTGGTAACAATTCTGTAGAAGGGATGCCGGAGTTATCTGAGGCAGACATGGTCATATTTCTTGGTGATTTTAACTATCGGCTTGATGGTATATCTTATGATGAAGCTAGAGATTTTGTTTCTCAAAGATGCTTTGATTGGCTTAGAGAACGGGATCAGCTCCGAGTGGAAATGGCAGCTGGAAATGTCTTTCAAGGAATGCGGGAGGCAGATATTACATTTCCTCCCACATACAAGTTTGAAAGGCATCAAGCTGGTTTAGCAGGGTATGATTCTGGTGAAAAGAAGCGCATTCCAGCTTGGTGTGATAGAATCCTGCATCGTGATAGCCGCTCAGCGTTGGTATCAGAATGCTGCTTAGAATGTCCTGTAGTCTCTTCAATATCACAGTACGAGGCTTCCATGGATGTAACCGACAGTGATCACAAGCCTGTCCGCTGCATTTTTACTCTTGATATTGCTCGAGTTGATGAGTCAATAAGGAGACAAGAGCTTGGAGATATCCTTGAATCAAATGGGAAACTCAAATGTATGCTTGAAGAACTATGCAGGATTCCTGAAACTATTGTCAGTACAAACAAAATAATCCTTCAAATCCAGGACACATCCATTTTGCGTATTACAAATAAATCTGGCCAAAAAGATGCTTTATTTGAAATAATTTGTGAAGGACAATCTATCATTAAGGAGGATGGACGTGCATCAGATCATTGTCCCAGAGGTTCCTTTGGCTTTCCGCGATGGCTTCAGGTCACTCCTGCAGCTGGCATAATCAAACCAGATCATATAGCAGAGGTGTCTGTTCATCATGAGGAACACCAAACCCTAGAAGAGTTTGTTGATGGTGTCCCGCAGAACTGTTGGTGTGAAAACACCAGAGACAAGGAAGTTATACTGGTGGTTAAGGTACACGGGAGGTACAGTAATAACACCAAAAGCCATCGCGTCTGTGTGCGTCACTGCTGTTCAGCCAAGACAAAGCAAAGTGACCCCCCCGAGCTCAACACCAGACAAACCCAAGGAACCGCTCTTCACCGTTCTAACTTCCAACACCTTAGCAGTTCCTATAACGTGGTTGACCACCTGTGGAGTATGAATAGTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

1122

Amino Acids

123.75

Weight (kDa)

5.75

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_IP5PC_F PF23754 141 - 531 9.4e-187 IP5P C-F beta-propeller
Exo_endo_phos2 PF22669 565 - 900 1.7e-65 Endonuclease/Exonuclease/phosphatase family 2
Exo_endo_phos PF03372 565 - 896 5e-07 Endonuclease/Exonuclease/phosphatase family
Ig-like_IP5PC_F PF23755 939 - 1075 1.7e-70 IP5P C-F Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2124
Acc65I GGTACC 1 cut(s) 341
AccB1I GGYRCC 4 cut(s) 341, 538, 580, 1262
AccB7I CCANNNNNTGG 1 cut(s) 1474
AccBSI CCGCTC 2 cut(s) 2595, 3290
AccI GTMKAC 2 cut(s) 252, 2007
AccII CGCG 7 cut(s) 53, 611, 1060, 2040, 2250, 3001, 3207
AclWI GGATC 5 cut(s) 810, 923, 1929, 1942, 2424
AcoI YGGCCR 4 cut(s) 389, 842, 1566, 2896
AcsI RAATTY 4 cut(s) 514, 1148, 1385, 1652
AcuI CTGAAG 3 cut(s) 960, 1311, 2993
AcyI GRCGYC 3 cut(s) 144, 282, 539
AfiI CCNNNNNNNGG 5 cut(s) 367, 480, 481, 1474, 3026
AflIII ACRYGT 2 cut(s) 894, 1927
AhlI ACTAGT 1 cut(s) 718
AjiI CACGTC 1 cut(s) 2960
AjnI CCWGG 4 cut(s) 932, 1467, 1742, 2859
Alw21I GWGCWC 3 cut(s) 605, 1865, 3264
Alw44I GTGCAC 1 cut(s) 601
AlwI GGATC 5 cut(s) 810, 923, 1929, 1942, 2424
AlwNI CAGNNNCTG 2 cut(s) 1238, 1547
Ama87I CYCGRG 2 cut(s) 2724, 3257
AoxI GGCC 8 cut(s) 327, 389, 410, 766, 842, 1342, 1566, 2896
ApaLI GTGCAC 1 cut(s) 601
ApoI RAATTY 4 cut(s) 514, 1148, 1385, 1652
ArsI GACNNNNNNTTYG 4 cut(s) 67, 99, 1980, 2012
Asp700I GAANNNNTTC 1 cut(s) 1082
Asp718I GGTACC 1 cut(s) 341
AspLEI GCGC 9 cut(s) 55, 500, 541, 1060, 1062, 2040, 2042, 2125, 2553
AspS9I GGNCC 7 cut(s) 376, 411, 767, 936, 1160, 1477, 1610
AsuC2I CCSGG 2 cut(s) 362, 1608
AsuHPI GGTGA 7 cut(s) 108, 625, 1596, 1984, 2339, 2552, 3287
AvaI CYCGRG 2 cut(s) 2724, 3257
AvaII GGWCC 5 cut(s) 376, 936, 1160, 1477, 1610
BaeGI GKGCMC 1 cut(s) 605
BalI TGGCCA 3 cut(s) 844, 1568, 2898
BamHI GGATCC 1 cut(s) 1934
BanI GGYRCC 4 cut(s) 341, 538, 580, 1262
BanII GRGCYC 3 cut(s) 305, 1865, 3264
BbsI GAAGAC 1 cut(s) 891
Bbv12I GWGCWC 3 cut(s) 605, 1865, 3264
BbvCI CCTCAGC 3 cut(s) 422, 546, 712
BceAI ACGGC 6 cut(s) 106, 131, 244, 485, 594, 932
BcgI CGANNNNNNTGC 2 cut(s) 85, 119
BciT130I CCWGG 4 cut(s) 934, 1469, 1744, 2861
BclI TGATCA 1 cut(s) 2683
BcnI CCSGG 2 cut(s) 362, 1608
BcuI ACTAGT 1 cut(s) 718
BfaI CTAG 5 cut(s) 719, 1748, 2220, 2370, 3089
BfmI CTRYAG 7 cut(s) 1539, 1830, 1845, 2013, 2277, 2631, 3021
BfoI RGCGCY 1 cut(s) 542
BglI GCCNNNNNGGC 1 cut(s) 397
BglII AGATCT 1 cut(s) 523
BlpI GCTNAGC 1 cut(s) 2596
Bme1390I CCNGG 6 cut(s) 362, 934, 1469, 1608, 1744, 2861
Bme18I GGWCC 5 cut(s) 376, 936, 1160, 1477, 1610
BmeT110I CYCGRG 2 cut(s) 2724, 3257
BmgBI CACGTC 1 cut(s) 2960
BmgT120I GGNCC 7 cut(s) 376, 411, 767, 936, 1160, 1477, 1610
BmrFI CCNGG 6 cut(s) 362, 934, 1469, 1608, 1744, 2861
BmsI GCATC 9 cut(s) 431, 1045, 1225, 2277, 2381, 2518, 2590, 2896, 2972
BoxI GACNNNNGTC 3 cut(s) 458, 1518, 1720
BpiI GAAGAC 1 cut(s) 891
BplI GAGNNNNNCTC 2 cut(s) 1847, 1879
BpmI CTGGAG 1 cut(s) 991
Bpu10I CCTNAGC 5 cut(s) 297, 422, 546, 712, 3317
Bpu1102I GCTNAGC 1 cut(s) 2596
BpuEI CTTGAG 1 cut(s) 1874
BpuMI CCSGG 2 cut(s) 362, 1608
BsaAI YACGTR 2 cut(s) 1211, 1436
BsaBI GATNNNNATC 3 cut(s) 1278, 1488, 2160
BsaHI GRCGYC 3 cut(s) 144, 282, 539
BsaI GGTCTC 2 cut(s) 89, 422
BsaJI CCNNGG 8 cut(s) 330, 395, 609, 845, 932, 1563, 2664, 3280
BsaWI WCCGGW 1 cut(s) 344
Bsc4I CCNNNNNNNGG 5 cut(s) 367, 480, 481, 1474, 3026
Bse118I RCCGGY 3 cut(s) 387, 535, 541
Bse1I ACTGG 3 cut(s) 1008, 1696, 3162
Bse3DI GCAATG 1 cut(s) 1959
Bse8I GATNNNNATC 3 cut(s) 1278, 1488, 2160
BseBI CCWGG 4 cut(s) 934, 1469, 1744, 2861
BseDI CCNNGG 8 cut(s) 330, 395, 609, 845, 932, 1563, 2664, 3280
BseJI GATNNNNATC 3 cut(s) 1278, 1488, 2160
BseLI CCNNNNNNNGG 5 cut(s) 367, 480, 481, 1474, 3026
BseMI GCAATG 1 cut(s) 1959
BseNI ACTGG 3 cut(s) 1008, 1696, 3162
BsePI GCGCGC 2 cut(s) 1058, 2038
BseRI GAGGAG 8 cut(s) 37, 295, 298, 688, 691, 694, 697, 700
BseSI GKGCMC 1 cut(s) 605
BseX3I CGGCCG 1 cut(s) 389
BseYI CCCAGC 1 cut(s) 485
BsgI GTGCAG 2 cut(s) 1373, 2232
Bsh1236I CGCG 7 cut(s) 53, 611, 1060, 2040, 2250, 3001, 3207
Bsh1285I CGRYCG 2 cut(s) 202, 392
BshFI GGCC 8 cut(s) 329, 391, 412, 768, 844, 1344, 1568, 2898
BshNI GGYRCC 4 cut(s) 341, 538, 580, 1262
BsiEI CGRYCG 2 cut(s) 202, 392
BsiHKAI GWGCWC 3 cut(s) 605, 1865, 3264
BsiHKCI CYCGRG 2 cut(s) 2724, 3257
BsiSI CCGG 7 cut(s) 345, 362, 388, 536, 542, 1607, 2291
BslFI GGGAC 6 cut(s) 344, 457, 502, 1623, 2961, 3095
BslI CCNNNNNNNGG 5 cut(s) 367, 480, 481, 1474, 3026
BsmBI CGTCTC 3 cut(s) 33, 154, 1728
BsmFI GGGAC 6 cut(s) 344, 457, 502, 1623, 2961, 3095
BsmI GAATGC 3 cut(s) 2466, 2553, 2618
BsnI GGCC 8 cut(s) 329, 391, 412, 768, 844, 1344, 1568, 2898
Bso31I GGTCTC 2 cut(s) 89, 422
BsoBI CYCGRG 2 cut(s) 2724, 3257
Bsp1286I GDGCHC 4 cut(s) 305, 605, 1865, 3264
Bsp1407I TGTACA 1 cut(s) 1334
Bsp1720I GCTNAGC 1 cut(s) 2596
Bsp19I CCATGG 2 cut(s) 845, 2664
BspANI GGCC 8 cut(s) 329, 391, 412, 768, 844, 1344, 1568, 2898
BspFNI CGCG 7 cut(s) 53, 611, 1060, 2040, 2250, 3001, 3207
BspHI TCATGA 1 cut(s) 3070
BspMAI CTGCAG 3 cut(s) 1834, 2017, 3025
BspPI GGATC 5 cut(s) 810, 923, 1929, 1942, 2424
BspQI GCTCTTC 1 cut(s) 3297
BspT107I GGYRCC 4 cut(s) 341, 538, 580, 1262
BspTNI GGTCTC 2 cut(s) 89, 422
BsrBI CCGCTC 2 cut(s) 2595, 3290
BsrDI GCAATG 1 cut(s) 1959
BsrFI RCCGGY 3 cut(s) 387, 535, 541
BsrGI TGTACA 1 cut(s) 1334
BsrI ACTGG 3 cut(s) 1008, 1696, 3162
BssAI RCCGGY 3 cut(s) 387, 535, 541
BssECI CCNNGG 8 cut(s) 330, 395, 609, 845, 932, 1563, 2664, 3280
BssHII GCGCGC 2 cut(s) 1058, 2038
BssNI GRCGYC 3 cut(s) 144, 282, 539
BssT1I CCWWGG 6 cut(s) 330, 395, 845, 1563, 2664, 3280
Bst2UI CCWGG 4 cut(s) 934, 1469, 1744, 2861
Bst6I CTCTTC 5 cut(s) 107, 118, 2644, 3087, 3297
BstACI GRCGYC 3 cut(s) 144, 282, 539
BstAPI GCANNNNNTGC 1 cut(s) 1235
BstAUI TGTACA 1 cut(s) 1334
BstBAI YACGTR 2 cut(s) 1211, 1436
BstC8I GCNNGC 9 cut(s) 206, 537, 543, 756, 1060, 1379, 1645, 2040, 3028
BstDSI CCRYGG 3 cut(s) 609, 845, 2664
BstFNI CGCG 7 cut(s) 53, 611, 1060, 2040, 2250, 3001, 3207
BstH2I RGCGCY 1 cut(s) 542
BstHHI GCGC 9 cut(s) 55, 500, 541, 1060, 1062, 2040, 2042, 2125, 2553
BstMCI CGRYCG 2 cut(s) 202, 392
BstNI CCWGG 4 cut(s) 934, 1469, 1744, 2861
BstNSI RCATGY 2 cut(s) 898, 1381
BstPAI GACNNNNGTC 3 cut(s) 458, 1518, 1720
BstSCI CCNGG 6 cut(s) 360, 932, 1467, 1606, 1742, 2859
BstSFI CTRYAG 7 cut(s) 1539, 1830, 1845, 2013, 2277, 2631, 3021
BstSLI GKGCMC 1 cut(s) 605
BstSNI TACGTA 1 cut(s) 1436
BstUI CGCG 7 cut(s) 53, 611, 1060, 2040, 2250, 3001, 3207
BstV2I GAAGAC 1 cut(s) 891
BstX2I RGATCY 3 cut(s) 523, 802, 1934
BstXI CCANNNNNNTGG 2 cut(s) 80, 1945
BstYI RGATCY 3 cut(s) 523, 802, 1934
BstZI CGGCCG 1 cut(s) 389
BsuRI GGCC 8 cut(s) 329, 391, 412, 768, 844, 1344, 1568, 2898
BtgI CCRYGG 3 cut(s) 609, 845, 2664
BtgZI GCGATG 2 cut(s) 3016, 3188
BtrI CACGTC 1 cut(s) 2960
BtsI GCAGTG 5 cut(s) 291, 1260, 1361, 1880, 3220
Cac8I GCNNGC 9 cut(s) 206, 537, 543, 756, 1060, 1379, 1645, 2040, 3028
CaiI CAGNNNCTG 2 cut(s) 1238, 1547
CciI TCATGA 1 cut(s) 3070
CfoI GCGC 9 cut(s) 55, 500, 541, 1060, 1062, 2040, 2042, 2125, 2553
Cfr10I RCCGGY 3 cut(s) 387, 535, 541
Cfr13I GGNCC 7 cut(s) 376, 411, 767, 936, 1160, 1477, 1610
Cfr42I CCGCGG 1 cut(s) 612
CseI GACGC 6 cut(s) 152, 290, 1710, 2018, 3196, 3206
CspCI CAANNNNNGTGG 2 cut(s) 1558, 1593
DinI GGCGCC 1 cut(s) 540
DraI TTTAAA 1 cut(s) 520
EaeI YGGCCR 4 cut(s) 389, 842, 1566, 2896
EagI CGGCCG 1 cut(s) 389
Eam1104I CTCTTC 5 cut(s) 107, 118, 2644, 3087, 3297
EarI CTCTTC 5 cut(s) 107, 118, 2644, 3087, 3297
Ecl136II GAGCTC 2 cut(s) 1863, 3262
EclXI CGGCCG 1 cut(s) 389
Eco105I TACGTA 1 cut(s) 1436
Eco130I CCWWGG 6 cut(s) 330, 395, 845, 1563, 2664, 3280
Eco24I GRGCYC 3 cut(s) 305, 1865, 3264
Eco31I GGTCTC 2 cut(s) 89, 422
Eco32I GATATC 1 cut(s) 2764
Eco47I GGWCC 5 cut(s) 376, 936, 1160, 1477, 1610
Eco52I CGGCCG 1 cut(s) 389
Eco53kI GAGCTC 2 cut(s) 1863, 3262
Eco57I CTGAAG 3 cut(s) 960, 1311, 2993
Eco88I CYCGRG 2 cut(s) 2724, 3257
EcoICRI GAGCTC 2 cut(s) 1863, 3262
EcoO109I RGGNCCY 3 cut(s) 411, 767, 1610
EcoRI GAATTC 1 cut(s) 1385
EcoRII CCWGG 4 cut(s) 932, 1467, 1742, 2859
EcoRV GATATC 1 cut(s) 2764
EcoT14I CCWWGG 6 cut(s) 330, 395, 845, 1563, 2664, 3280
EcoT38I GRGCYC 3 cut(s) 305, 1865, 3264
EgeI GGCGCC 1 cut(s) 540
EheI GGCGCC 1 cut(s) 540
ErhI CCWWGG 6 cut(s) 330, 395, 845, 1563, 2664, 3280
Esp3I CGTCTC 3 cut(s) 33, 154, 1728
FalI AAGNNNNNCTT 4 cut(s) 728, 760, 1250, 1282
FaqI GGGAC 6 cut(s) 344, 457, 502, 1623, 2961, 3095
FauI CCCGC 2 cut(s) 2457, 3120
FbaI TGATCA 1 cut(s) 2683
FblI GTMKAC 2 cut(s) 252, 2007
FriOI GRGCYC 3 cut(s) 305, 1865, 3264
FspBI CTAG 5 cut(s) 719, 1748, 2220, 2370, 3089
FspI TGCGCA 1 cut(s) 2124
GlaI GCGC 9 cut(s) 54, 499, 540, 1059, 1061, 2039, 2041, 2124, 2552
GsaI CCCAGC 1 cut(s) 489
GsuI CTGGAG 1 cut(s) 991
HaeII RGCGCY 1 cut(s) 542
HaeIII GGCC 8 cut(s) 329, 391, 412, 768, 844, 1344, 1568, 2898
HapII CCGG 7 cut(s) 345, 362, 388, 536, 542, 1607, 2291
HgaI GACGC 6 cut(s) 152, 290, 1710, 2018, 3196, 3206
HhaI GCGC 9 cut(s) 55, 500, 541, 1060, 1062, 2040, 2042, 2125, 2553
Hin1I GRCGYC 3 cut(s) 144, 282, 539
Hin6I GCGC 9 cut(s) 53, 498, 539, 1058, 1060, 2038, 2040, 2123, 2551
HinP1I GCGC 9 cut(s) 53, 498, 539, 1058, 1060, 2038, 2040, 2123, 2551
HincII GTYRAC 7 cut(s) 226, 238, 244, 253, 1917, 2008, 3340
HindII GTYRAC 7 cut(s) 226, 238, 244, 253, 1917, 2008, 3340
HpaII CCGG 7 cut(s) 345, 362, 388, 536, 542, 1607, 2291
HphI GGTGA 7 cut(s) 108, 625, 1596, 1984, 2339, 2552, 3287
Hpy99I CGWCG 7 cut(s) 17, 92, 251, 254, 284, 1248, 2012
HpyAV CCTTC 7 cut(s) 557, 1252, 1904, 2194, 2276, 2861, 2924
HpyCH4IV ACGT 9 cut(s) 222, 234, 240, 246, 1210, 1435, 1927, 2959, 3333
HpySE526I ACGT 9 cut(s) 222, 234, 240, 246, 1210, 1435, 1927, 2959, 3333
Hsp92I GRCGYC 3 cut(s) 144, 282, 539
HspAI GCGC 9 cut(s) 53, 498, 539, 1058, 1060, 2038, 2040, 2123, 2551
KasI GGCGCC 1 cut(s) 538
KpnI GGTACC 1 cut(s) 345
KroI GCCGGC 2 cut(s) 535, 541
KroNI GCCGGC 2 cut(s) 537, 543
Ksp22I TGATCA 1 cut(s) 2683
KspI CCGCGG 1 cut(s) 612
LguI GCTCTTC 1 cut(s) 3297
LweI GCATC 9 cut(s) 431, 1045, 1225, 2277, 2381, 2518, 2590, 2896, 2972
MaeI CTAG 5 cut(s) 719, 1748, 2220, 2370, 3089
MaeII ACGT 9 cut(s) 222, 234, 240, 246, 1210, 1435, 1927, 2959, 3333
MbiI CCGCTC 2 cut(s) 2595, 3290
MfeI CAATTG 2 cut(s) 476, 2206
MflI RGATCY 3 cut(s) 523, 802, 1934
MhlI GDGCHC 4 cut(s) 305, 605, 1865, 3264
MlsI TGGCCA 3 cut(s) 844, 1568, 2898
MluNI TGGCCA 3 cut(s) 844, 1568, 2898
Mly113I GGCGCC 1 cut(s) 539
MlyI GAGTC 6 cut(s) 104, 479, 602, 689, 1724, 2744
MmeI TCCRAC 5 cut(s) 665, 1687, 1912, 1978, 3334
Mox20I TGGCCA 3 cut(s) 844, 1568, 2898
MreI CGCCGGCG 1 cut(s) 535
MroNI GCCGGC 2 cut(s) 535, 541
MroXI GAANNNNTTC 1 cut(s) 1082
MscI TGGCCA 3 cut(s) 844, 1568, 2898
MslI CAYNNNNRTG 2 cut(s) 666, 1964
Msp20I TGGCCA 3 cut(s) 844, 1568, 2898
MspA1I CMGCKG 6 cut(s) 421, 611, 2216, 2441, 2700, 3026
MspI CCGG 7 cut(s) 345, 362, 388, 536, 542, 1607, 2291
MspR9I CCNGG 6 cut(s) 362, 934, 1469, 1608, 1744, 2861
MunI CAATTG 2 cut(s) 476, 2206
Mva1269I GAATGC 3 cut(s) 2466, 2553, 2618
MvaI CCWGG 4 cut(s) 934, 1469, 1744, 2861
MvnI CGCG 7 cut(s) 53, 611, 1060, 2040, 2250, 3001, 3207
NaeI GCCGGC 2 cut(s) 537, 543
NarI GGCGCC 1 cut(s) 539
NciI CCSGG 2 cut(s) 362, 1608
NcoI CCATGG 2 cut(s) 845, 2664
NgoMIV GCCGGC 2 cut(s) 535, 541
NmuCI GTSAC 4 cut(s) 2111, 3013, 3218, 3248
NsbI TGCGCA 1 cut(s) 2124
NspI RCATGY 2 cut(s) 898, 1381
PaeI GCATGC 1 cut(s) 1381
PaeR7I CTCGAG 1 cut(s) 2724
PagI TCATGA 1 cut(s) 3070
PauI GCGCGC 2 cut(s) 1058, 2038
PciI ACATGT 1 cut(s) 894
PciSI GCTCTTC 1 cut(s) 3297
PcsI WCGNNNNNNNCGW 4 cut(s) 84, 246, 2004, 2157
PctI GAATGC 3 cut(s) 2466, 2553, 2618
PdiI GCCGGC 2 cut(s) 537, 543
PdmI GAANNNNTTC 1 cut(s) 1082
PfeI GAWTC 6 cut(s) 569, 1108, 2534, 2574, 2771, 2815
PflFI GACNNNGTC 2 cut(s) 146, 2312
PflMI CCANNNNNTGG 1 cut(s) 1474
PfoI TCCNGGA 1 cut(s) 2859
PleI GAGTC 6 cut(s) 104, 478, 601, 689, 1724, 2743
PluTI GGCGCC 1 cut(s) 542
PpsI GAGTC 6 cut(s) 104, 478, 601, 689, 1724, 2743
Ppu21I YACGTR 2 cut(s) 1211, 1436
PpuMI RGGWCCY 1 cut(s) 1610
PscI ACATGT 1 cut(s) 894
PshAI GACNNNNGTC 3 cut(s) 458, 1518, 1720
Psp124BI GAGCTC 2 cut(s) 1865, 3264
Psp5II RGGWCCY 1 cut(s) 1610
Psp6I CCWGG 4 cut(s) 932, 1467, 1742, 2859
PspFI CCCAGC 1 cut(s) 485
PspGI CCWGG 4 cut(s) 932, 1467, 1742, 2859
PspPI GGNCC 7 cut(s) 376, 411, 767, 936, 1160, 1477, 1610
PspPPI RGGWCCY 1 cut(s) 1610
PspXI VCTCGAGB 1 cut(s) 2724
PstI CTGCAG 3 cut(s) 1834, 2017, 3025
PstNI CAGNNNCTG 2 cut(s) 1238, 1547
PsuI RGATCY 3 cut(s) 523, 802, 1934
PsyI GACNNNGTC 2 cut(s) 146, 2312
PteI GCGCGC 2 cut(s) 1058, 2038
PvuII CAGCTG 3 cut(s) 2216, 2441, 3026
RseI CAYNNNNRTG 2 cut(s) 666, 1964
SacI GAGCTC 2 cut(s) 1865, 3264
SacII CCGCGG 1 cut(s) 612
SalI GTCGAC 2 cut(s) 251, 2006
SapI GCTCTTC 1 cut(s) 3297
Sau96I GGNCC 7 cut(s) 376, 411, 767, 936, 1160, 1477, 1610
SchI GAGTC 6 cut(s) 104, 479, 602, 689, 1724, 2744
ScrFI CCNGG 6 cut(s) 362, 934, 1469, 1608, 1744, 2861
SduI GDGCHC 4 cut(s) 305, 605, 1865, 3264
SfaNI GCATC 9 cut(s) 431, 1045, 1225, 2277, 2381, 2518, 2590, 2896, 2972
SfcI CTRYAG 7 cut(s) 1539, 1830, 1845, 2013, 2277, 2631, 3021
SfoI GGCGCC 1 cut(s) 540
Sfr274I CTCGAG 1 cut(s) 2724
Sfr303I CCGCGG 1 cut(s) 612
SgrAI CRCCGGYG 1 cut(s) 535
SgrBI CCGCGG 1 cut(s) 612
SinI GGWCC 5 cut(s) 376, 936, 1160, 1477, 1610
SlaI CTCGAG 1 cut(s) 2724
SmiMI CAYNNNNRTG 2 cut(s) 666, 1964
SmlI CTYRAG 2 cut(s) 1853, 2724
SmoI CTYRAG 2 cut(s) 1853, 2724
SnaBI TACGTA 1 cut(s) 1436
SpeI ACTAGT 1 cut(s) 718
SphI GCATGC 1 cut(s) 1381
SspDI GGCGCC 1 cut(s) 538
SspI AATATT 2 cut(s) 1597, 1686
SspMI CTAG 5 cut(s) 719, 1748, 2220, 2370, 3089
SstI GAGCTC 2 cut(s) 1865, 3264
StyD4I CCNGG 6 cut(s) 360, 932, 1467, 1606, 1742, 2859
StyI CCWWGG 6 cut(s) 330, 395, 845, 1563, 2664, 3280
TaiI ACGT 9 cut(s) 225, 237, 243, 249, 1213, 1438, 1930, 2962, 3336
TatI WGTACW 4 cut(s) 528, 1334, 1421, 2834
TauI GCSGC 6 cut(s) 96, 287, 374, 394, 2148, 2595
TfiI GAWTC 6 cut(s) 569, 1108, 2534, 2574, 2771, 2815
TseFI GTSAC 4 cut(s) 2111, 3013, 3218, 3248
Tsp45I GTSAC 4 cut(s) 2111, 3013, 3218, 3248
TspDTI ATGAA 7 cut(s) 114, 150, 1113, 1923, 2217, 2379, 3056
TspGWI ACGGA 5 cut(s) 29, 202, 506, 586, 2013
Tth111I GACNNNGTC 2 cut(s) 146, 2312
Van91I CCANNNNNTGG 1 cut(s) 1474
VneI GTGCAC 1 cut(s) 601
VpaK11BI GGWCC 5 cut(s) 376, 936, 1160, 1477, 1610
XapI RAATTY 4 cut(s) 514, 1148, 1385, 1652
XceI RCATGY 2 cut(s) 898, 1381
XcmI CCANNNNNNNNNTGG 1 cut(s) 482
XhoI CTCGAG 1 cut(s) 2724
XmiI GTMKAC 2 cut(s) 252, 2007
XmnI GAANNNNTTC 1 cut(s) 1082
XspI CTAG 5 cut(s) 719, 1748, 2220, 2370, 3089
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.