pycom11g09900

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0001934
Physical Location & Seq
Reverse (-)
8301982 .. 8302605
624 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g09900.1

Sequence Viewer

Length: 624 bp
ATGGCAGTTCCAATGCTTAAGCTCGTCATTACCCTTTTCTTTCTGCTAGCCCTCCTCACATCCCATATCCAAGCCGGCCCGGGAAACTCCGATCTCTTTCGCGAATACATAGGAGCCGAATTCAATAACGTCAAGTTTTCGGATGTCCCCATAAACCCTAATGTAGAATTCCACTTCATTCTCTCCTTTGCCATAGACTATGACCCCTCAGGCTCCTCTCCCACCAACGGAAAATTCAATGTCTTTTGGGACTCCGACAACCTCAGCCCTTCCCAAGTTTCCTCCATCAAAAACCAACATTCTAATGTCAAAGTTGCCTTGAGCTTAGGAGGAGATAGTGTCAATAGTGGCTTTGCCTACTTCAACCCTTCATCGATTGATTCGTGGGTTTCAAATGCTGTTTCTTCACTCACAAGCATCATCCAGCAGTACAACTTGGACGGAATCGACATTGATTACGAGCACTTTCAGGCAGACCCCGATACATTTTCCGAGTGCATTGGAAGGCTTATAACAACCCTAAAGAACAATGGAGTGATCGATCATTTGCTTCTATTGCTCCGTTTGACGACGATGAAGTTCAGGGCCACTACTTGGCCTTGTGGAAGAGCTATGGCCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

208

Amino Acids

22.85

Weight (kDa)

5.01

Isoelectric Point (pI)

25.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 83 - 175 1.5e-11 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 512
AccB7I CCANNNNNTGG 1 cut(s) 594
AccII CGCG 1 cut(s) 102
AcoI YGGCCR 1 cut(s) 615
AcsI RAATTY 3 cut(s) 119, 167, 233
AfaI GTAC 1 cut(s) 431
AfiI CCNNNNNNNGG 2 cut(s) 227, 594
AflII CTTAAG 1 cut(s) 17
AgsI TTSAA 4 cut(s) 124, 238, 364, 393
AjuI GAANNNNNNNTTGG 2 cut(s) 218, 250
AluBI AGCT 3 cut(s) 22, 324, 611
AluI AGCT 3 cut(s) 22, 324, 611
Alw21I GWGCWC 1 cut(s) 465
Ama87I CYCGRG 1 cut(s) 79
AoxI GGCC 4 cut(s) 76, 585, 596, 615
ApoI RAATTY 3 cut(s) 119, 167, 233
AspS9I GGNCC 2 cut(s) 77, 585
AsuC2I CCSGG 2 cut(s) 80, 81
AsuNHI GCTAGC 1 cut(s) 46
AvaI CYCGRG 1 cut(s) 79
AxyI CCTNAGG 1 cut(s) 208
BalI TGGCCA 1 cut(s) 617
Bbv12I GWGCWC 1 cut(s) 465
BbvCI CCTCAGC 1 cut(s) 263
BccI CCATC 1 cut(s) 293
BcnI CCSGG 2 cut(s) 80, 81
BfaI CTAG 1 cut(s) 47
BfrI CTTAAG 1 cut(s) 17
Bme1390I CCNGG 2 cut(s) 80, 81
BmeT110I CYCGRG 1 cut(s) 79
BmgT120I GGNCC 2 cut(s) 77, 585
BmiI GGNNCC 2 cut(s) 115, 214
BmrFI CCNGG 2 cut(s) 80, 81
BmsI GCATC 1 cut(s) 426
BmtI GCTAGC 1 cut(s) 50
Bpu10I CCTNAGC 2 cut(s) 263, 325
BpuEI CTTGAG 1 cut(s) 340
BpuMI CCSGG 2 cut(s) 80, 81
Bsa29I ATCGAT 2 cut(s) 374, 540
BsaJI CCNNGG 1 cut(s) 79
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bsc4I CCNNNNNNNGG 2 cut(s) 227, 594
Bse118I RCCGGY 1 cut(s) 74
Bse21I CCTNAGG 1 cut(s) 208
BseCI ATCGAT 2 cut(s) 374, 540
BseDI CCNNGG 1 cut(s) 79
BseGI GGATG 3 cut(s) 59, 148, 420
BseLI CCNNNNNNNGG 2 cut(s) 227, 594
BseMII CTCAG 2 cut(s) 222, 277
BseRI GAGGAG 3 cut(s) 44, 205, 345
Bsh1236I CGCG 1 cut(s) 102
BshFI GGCC 4 cut(s) 78, 587, 598, 617
BshVI ATCGAT 2 cut(s) 374, 540
BsiHKAI GWGCWC 1 cut(s) 465
BsiHKCI CYCGRG 1 cut(s) 79
BsiSI CCGG 2 cut(s) 75, 80
BslFI GGGAC 2 cut(s) 131, 263
BslI CCNNNNNNNGG 2 cut(s) 227, 594
BsmFI GGGAC 2 cut(s) 131, 263
BsnI GGCC 4 cut(s) 78, 587, 598, 617
BsoBI CYCGRG 1 cut(s) 79
Bsp1286I GDGCHC 1 cut(s) 465
Bsp143I GATC 3 cut(s) 91, 537, 541
Bsp68I TCGCGA 1 cut(s) 102
BspANI GGCC 4 cut(s) 78, 587, 598, 617
BspCNI CTCAG 2 cut(s) 221, 276
BspDI ATCGAT 2 cut(s) 374, 540
BspFNI CGCG 1 cut(s) 102
BspLI GGNNCC 2 cut(s) 115, 214
BspOI GCTAGC 1 cut(s) 50
BspQI GCTCTTC 1 cut(s) 601
BspTI CTTAAG 1 cut(s) 17
BsrFI RCCGGY 1 cut(s) 74
BssAI RCCGGY 1 cut(s) 74
BssECI CCNNGG 1 cut(s) 79
BssMI GATC 3 cut(s) 91, 537, 541
Bst6I CTCTTC 1 cut(s) 601
BstAFI CTTAAG 1 cut(s) 17
BstC8I GCNNGC 2 cut(s) 48, 76
BstDEI CTNAG 3 cut(s) 208, 263, 325
BstF5I GGATG 3 cut(s) 59, 148, 420
BstFNI CGCG 1 cut(s) 102
BstKTI GATC 3 cut(s) 94, 540, 544
BstMBI GATC 3 cut(s) 91, 537, 541
BstMWI GCNNNNNNNGC 1 cut(s) 556
BstSCI CCNGG 2 cut(s) 78, 79
BstUI CGCG 1 cut(s) 102
Bsu15I ATCGAT 2 cut(s) 374, 540
Bsu36I CCTNAGG 1 cut(s) 208
BsuRI GGCC 4 cut(s) 78, 587, 598, 617
BsuTUI ATCGAT 2 cut(s) 374, 540
BtsCI GGATG 3 cut(s) 59, 148, 420
BtuMI TCGCGA 1 cut(s) 102
Cac8I GCNNGC 2 cut(s) 48, 76
Cfr10I RCCGGY 1 cut(s) 74
Cfr13I GGNCC 2 cut(s) 77, 585
Cfr9I CCCGGG 1 cut(s) 79
ClaI ATCGAT 2 cut(s) 374, 540
Csp6I GTAC 1 cut(s) 430
CviQI GTAC 1 cut(s) 430
DdeI CTNAG 3 cut(s) 208, 263, 325
DpnI GATC 3 cut(s) 93, 539, 543
DpnII GATC 3 cut(s) 91, 537, 541
EaeI YGGCCR 1 cut(s) 615
Eam1104I CTCTTC 1 cut(s) 601
EarI CTCTTC 1 cut(s) 601
Eco81I CCTNAGG 1 cut(s) 208
Eco88I CYCGRG 1 cut(s) 79
EcoRI GAATTC 2 cut(s) 119, 167
FaiI YATR 7 cut(s) 66, 110, 152, 194, 201, 512, 614
FaqI GGGAC 2 cut(s) 131, 263
FokI GGATG 3 cut(s) 46, 155, 407
FspBI CTAG 1 cut(s) 47
HaeIII GGCC 4 cut(s) 78, 587, 598, 617
HapII CCGG 2 cut(s) 75, 80
HinfI GANTC 3 cut(s) 251, 380, 444
HpaII CCGG 2 cut(s) 75, 80
Hpy188I TCNGA 4 cut(s) 91, 142, 256, 493
Hpy188III TCNNGA 1 cut(s) 101
Hpy99I CGWCG 1 cut(s) 574
HpyAV CCTTC 3 cut(s) 279, 378, 498
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 1 cut(s) 498
HpyF10VI GCNNNNNNNGC 1 cut(s) 556
HpyF3I CTNAG 3 cut(s) 208, 263, 325
HpySE526I ACGT 1 cut(s) 129
KroI GCCGGC 1 cut(s) 74
KroNI GCCGGC 1 cut(s) 76
Kzo9I GATC 3 cut(s) 91, 537, 541
LguI GCTCTTC 1 cut(s) 601
LmnI GCTCC 3 cut(s) 113, 218, 564
LpnPI CCDG 6 cut(s) 88, 93, 195, 437, 455, 568
LweI GCATC 1 cut(s) 426
MaeI CTAG 1 cut(s) 47
MaeII ACGT 1 cut(s) 129
MalI GATC 3 cut(s) 93, 539, 543
MboI GATC 3 cut(s) 91, 537, 541
MboII GAAGA 2 cut(s) 396, 618
MhlI GDGCHC 1 cut(s) 465
MlsI TGGCCA 1 cut(s) 617
MluCI AATT 3 cut(s) 119, 167, 233
MluNI TGGCCA 1 cut(s) 617
MlyI GAGTC 1 cut(s) 245
MmeI TCCRAC 1 cut(s) 279
MnlI CCTC 7 cut(s) 62, 65, 217, 226, 272, 292, 323
Mox20I TGGCCA 1 cut(s) 617
MroNI GCCGGC 1 cut(s) 74
MscI TGGCCA 1 cut(s) 617
MseI TTAA 1 cut(s) 18
MslI CAYNNNNRTG 1 cut(s) 303
Msp20I TGGCCA 1 cut(s) 617
MspCI CTTAAG 1 cut(s) 17
MspI CCGG 2 cut(s) 75, 80
MspR9I CCNGG 2 cut(s) 80, 81
MvnI CGCG 1 cut(s) 102
MwoI GCNNNNNNNGC 1 cut(s) 556
NaeI GCCGGC 1 cut(s) 76
NciI CCSGG 2 cut(s) 80, 81
NdeII GATC 3 cut(s) 91, 537, 541
NgoMIV GCCGGC 1 cut(s) 74
NheI GCTAGC 1 cut(s) 46
NlaIV GGNNCC 2 cut(s) 115, 214
NruI TCGCGA 1 cut(s) 102
PciSI GCTCTTC 1 cut(s) 601
PcsI WCGNNNNNNNCGW 1 cut(s) 380
PdiI GCCGGC 1 cut(s) 76
PfeI GAWTC 2 cut(s) 380, 444
PflMI CCANNNNNTGG 1 cut(s) 594
PleI GAGTC 1 cut(s) 245
PpsI GAGTC 1 cut(s) 245
PsiI TTATAA 1 cut(s) 512
PspN4I GGNNCC 2 cut(s) 115, 214
PspPI GGNCC 2 cut(s) 77, 585
RruI TCGCGA 1 cut(s) 102
RsaI GTAC 1 cut(s) 431
RsaNI GTAC 1 cut(s) 430
RseI CAYNNNNRTG 1 cut(s) 303
SapI GCTCTTC 1 cut(s) 601
SaqAI TTAA 1 cut(s) 18
Sau3AI GATC 3 cut(s) 91, 537, 541
Sau96I GGNCC 2 cut(s) 77, 585
SchI GAGTC 1 cut(s) 245
ScrFI CCNGG 2 cut(s) 80, 81
SduI GDGCHC 1 cut(s) 465
SetI ASST 5 cut(s) 24, 132, 264, 326, 613
SfaNI GCATC 1 cut(s) 426
SmaI CCCGGG 1 cut(s) 81
SmiMI CAYNNNNRTG 1 cut(s) 303
SmlI CTYRAG 2 cut(s) 17, 319
SmoI CTYRAG 2 cut(s) 17, 319
Sse9I AATT 3 cut(s) 119, 167, 233
SspMI CTAG 1 cut(s) 47
StyD4I CCNGG 2 cut(s) 78, 79
TaiI ACGT 1 cut(s) 132
TaqI TCGA 3 cut(s) 374, 447, 540
TasI AATT 3 cut(s) 119, 167, 233
TatI WGTACW 1 cut(s) 429
TfiI GAWTC 2 cut(s) 380, 444
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TspDTI ATGAA 3 cut(s) 166, 360, 590
TspGWI ACGGA 3 cut(s) 243, 456, 551
TspMI CCCGGG 1 cut(s) 79
Van91I CCANNNNNTGG 1 cut(s) 594
Vha464I CTTAAG 1 cut(s) 17
XapI RAATTY 3 cut(s) 119, 167, 233
XmaI CCCGGG 1 cut(s) 79
XspI CTAG 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.