MD03G1105600.v1.1

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001934
Physical Location & Seq
Reverse (-)
9070607 .. 9071518
912 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1105600.v1.1.491

Sequence Viewer

Length: 912 bp
ATGGCAGTTCCAAAGCTTAAGCTCGTCATCGCCCTTTTCTTCCTTCAAGCCCTCCTCACATCCCATACCCAAGCCGGTCCGGCAAACTCCGATCTCTTTCGAGAATACATAGGAGCCGAATTCAACAACGTCAAGTTTTCGGATGTCCCCATAAACCCTAATGTCGAACTCCACTTCATCCTCTCCTTTGCCATAGACTATGACCCCTCAGGCTCCTCTCCCACCAACGGCAAATTCAATGTCTTTTGGGACTCCGACAACCTTAGCCCTTCCCAAGTTTCCTCCATAAAAAACCAACATTCTAACGTTAAAGTGGCCTTGAGCTTAGGAGGAGACAGTGTCGGCAGTGGCTCTGCCTACTTCAACCCTTCCTCGATTGATTCCTGGGTTTCAAATGCTGTTTCTTCACTCACAAGCATCATCCAGCAGTACAACTTGGACGGAATCGACATTGATTACGAGCACTTTCAAGCGGACCCCGATACATTTTCCGAGTGCATTGGAACGCTTATAACAACCCTAAAGAACAATGGAGTCATCTCATTTGCTTCCATTGCTCCGTTTGATGATGATCAAGTTCAGAGCCACTACTTGGCCTTGTGGAAGAGCTATGGCCATTTGATTGACTATGTCAACTTCCAATTTTATGCATACGATCAGGGCACCACGGTGTCTCAGTTCATCAATTATTTCAACACTCAGAGCTCCAATTACAACGGCGGACAGGTCTTGGCAAGCTTTATCAGTGACGGGAGCGGCGGATTGTCCCCGGAGAACGGTTTTTTTAAAGCCTGCCATAGGCTCAAGAGTGAGGGCAAACTTGGTGGTATCTTTGTTTGGTCTGCTGATGATTCCATGAAACTTGGTTTCCGCTACGAAAAGCAATCGCAAGCTCTCTTGGCCAATAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

304

Amino Acids

33.23

Weight (kDa)

4.74

Isoelectric Point (pI)

33.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 83 - 220 1.5e-13 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 512
AccB1I GGYRCC 1 cut(s) 662
AccB7I CCANNNNNTGG 1 cut(s) 592
AccBSI CCGCTC 1 cut(s) 756
AciI CCGC 5 cut(s) 473, 720, 756, 759, 871
AclI AACGTT 1 cut(s) 306
AcoI YGGCCR 2 cut(s) 613, 900
AcsI RAATTY 2 cut(s) 119, 233
AfaI GTAC 1 cut(s) 431
AfiI CCNNNNNNNGG 4 cut(s) 227, 592, 776, 798
AflII CTTAAG 1 cut(s) 17
AgsI TTSAA 7 cut(s) 47, 124, 238, 364, 393, 470, 694
AjnI CCWGG 1 cut(s) 383
AjuI GAANNNNNNNTTGG 2 cut(s) 218, 250
AleI CACNNNNGTG 1 cut(s) 668
AluBI AGCT 7 cut(s) 16, 22, 324, 609, 705, 738, 893
AluI AGCT 7 cut(s) 16, 22, 324, 609, 705, 738, 893
Alw21I GWGCWC 2 cut(s) 465, 707
Alw26I GTCTC 2 cut(s) 327, 678
AoxI GGCC 4 cut(s) 315, 594, 613, 900
ApoI RAATTY 2 cut(s) 119, 233
ArsI GACNNNNNNTTYG 2 cut(s) 225, 257
AspS9I GGNCC 2 cut(s) 77, 475
AsuC2I CCSGG 1 cut(s) 770
AvaII GGWCC 2 cut(s) 77, 475
AxyI CCTNAGG 1 cut(s) 208
BaeGI GKGCMC 1 cut(s) 665
BalI TGGCCA 2 cut(s) 615, 902
BanI GGYRCC 1 cut(s) 662
BanII GRGCYC 1 cut(s) 707
Bbv12I GWGCWC 2 cut(s) 465, 707
BceAI ACGGC 2 cut(s) 244, 733
BciT130I CCWGG 1 cut(s) 385
BclI TGATCA 1 cut(s) 571
BcnI CCSGG 1 cut(s) 770
BcoDI GTCTC 2 cut(s) 327, 678
BfaI CTAG 1 cut(s) 910
BfrI CTTAAG 1 cut(s) 17
BglI GCCNNNNNGGC 1 cut(s) 80
BisI GCNGC 1 cut(s) 757
BlsI GCNGC 1 cut(s) 758
Bme1390I CCNGG 2 cut(s) 385, 770
Bme18I GGWCC 2 cut(s) 77, 475
BmgT120I GGNCC 2 cut(s) 77, 475
BmiI GGNNCC 4 cut(s) 115, 214, 477, 664
BmrFI CCNGG 2 cut(s) 385, 770
BmsI GCATC 1 cut(s) 426
Bpu10I CCTNAGC 2 cut(s) 263, 325
BpuEI CTTGAG 2 cut(s) 340, 788
BpuMI CCSGG 1 cut(s) 770
BsaBI GATNNNNATC 1 cut(s) 570
BsaJI CCNNGG 3 cut(s) 384, 666, 768
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bsc4I CCNNNNNNNGG 4 cut(s) 227, 592, 776, 798
Bse118I RCCGGY 1 cut(s) 74
Bse21I CCTNAGG 1 cut(s) 208
Bse3DI GCAATG 1 cut(s) 552
Bse8I GATNNNNATC 1 cut(s) 570
BseBI CCWGG 1 cut(s) 385
BseDI CCNNGG 3 cut(s) 384, 666, 768
BseGI GGATG 4 cut(s) 59, 148, 177, 420
BseJI GATNNNNATC 1 cut(s) 570
BseLI CCNNNNNNNGG 4 cut(s) 227, 592, 776, 798
BseMI GCAATG 1 cut(s) 552
BseMII CTCAG 3 cut(s) 222, 689, 713
BseRI GAGGAG 3 cut(s) 44, 205, 345
BseSI GKGCMC 1 cut(s) 665
BshFI GGCC 4 cut(s) 317, 596, 615, 902
BshNI GGYRCC 1 cut(s) 662
BsiHKAI GWGCWC 2 cut(s) 465, 707
BsiSI CCGG 3 cut(s) 75, 80, 770
BslFI GGGAC 3 cut(s) 131, 263, 751
BslI CCNNNNNNNGG 4 cut(s) 227, 592, 776, 798
BsmAI GTCTC 2 cut(s) 327, 678
BsmFI GGGAC 3 cut(s) 131, 263, 751
BsnI GGCC 4 cut(s) 317, 596, 615, 902
Bsp1286I GDGCHC 3 cut(s) 465, 665, 707
Bsp143I GATC 3 cut(s) 91, 571, 655
BspACI CCGC 5 cut(s) 473, 720, 756, 759, 871
BspANI GGCC 4 cut(s) 317, 596, 615, 902
BspCNI CTCAG 3 cut(s) 221, 688, 712
BspLI GGNNCC 4 cut(s) 115, 214, 477, 664
BspQI GCTCTTC 1 cut(s) 599
BspT107I GGYRCC 1 cut(s) 662
BspTI CTTAAG 1 cut(s) 17
BsrBI CCGCTC 1 cut(s) 756
BsrDI GCAATG 1 cut(s) 552
BsrFI RCCGGY 1 cut(s) 74
BssAI RCCGGY 1 cut(s) 74
BssECI CCNNGG 3 cut(s) 384, 666, 768
BssMI GATC 3 cut(s) 91, 571, 655
Bst2UI CCWGG 1 cut(s) 385
Bst4CI ACNGT 3 cut(s) 338, 670, 779
Bst6I CTCTTC 1 cut(s) 599
BstAFI CTTAAG 1 cut(s) 17
BstC8I GCNNGC 3 cut(s) 736, 793, 891
BstDEI CTNAG 5 cut(s) 208, 263, 325, 675, 699
BstDSI CCRYGG 1 cut(s) 666
BstENI CCTNNNNNAGG 1 cut(s) 796
BstF5I GGATG 4 cut(s) 59, 148, 177, 420
BstKTI GATC 3 cut(s) 94, 574, 658
BstMAI GTCTC 2 cut(s) 327, 678
BstMBI GATC 3 cut(s) 91, 571, 655
BstMWI GCNNNNNNNGC 3 cut(s) 80, 554, 899
BstNI CCWGG 1 cut(s) 385
BstSCI CCNGG 2 cut(s) 383, 768
BstSLI GKGCMC 1 cut(s) 665
Bsu36I CCTNAGG 1 cut(s) 208
BsuRI GGCC 4 cut(s) 317, 596, 615, 902
BtgI CCRYGG 1 cut(s) 666
BtgZI GCGATG 1 cut(s) 13
BtsCI GGATG 4 cut(s) 59, 148, 177, 420
BtsI GCAGTG 1 cut(s) 352
BtsIMutI CAGTG 3 cut(s) 343, 352, 751
Cac8I GCNNGC 3 cut(s) 736, 793, 891
Cfr10I RCCGGY 1 cut(s) 74
Cfr13I GGNCC 2 cut(s) 77, 475
CpoI CGGWCCG 1 cut(s) 77
Csp6I GTAC 1 cut(s) 430
CspCI CAANNNNNGTGG 2 cut(s) 805, 840
CspI CGGWCCG 1 cut(s) 77
CviAII CATG 1 cut(s) 856
CviQI GTAC 1 cut(s) 430
DdeI CTNAG 5 cut(s) 208, 263, 325, 675, 699
DpnI GATC 3 cut(s) 93, 573, 657
DpnII GATC 3 cut(s) 91, 571, 655
DraI TTTAAA 1 cut(s) 787
EaeI YGGCCR 2 cut(s) 613, 900
Eam1104I CTCTTC 1 cut(s) 599
EarI CTCTTC 1 cut(s) 599
EciI GGCGGA 2 cut(s) 735, 774
Ecl136II GAGCTC 1 cut(s) 705
Eco24I GRGCYC 1 cut(s) 707
Eco47I GGWCC 2 cut(s) 77, 475
Eco53kI GAGCTC 1 cut(s) 705
Eco81I CCTNAGG 1 cut(s) 208
EcoICRI GAGCTC 1 cut(s) 705
EcoNI CCTNNNNNAGG 1 cut(s) 796
EcoRI GAATTC 1 cut(s) 119
EcoRII CCWGG 1 cut(s) 383
EcoT22I ATGCAT 1 cut(s) 652
EcoT38I GRGCYC 1 cut(s) 707
FaeI CATG 1 cut(s) 859
FaqI GGGAC 3 cut(s) 131, 263, 751
FatI CATG 1 cut(s) 855
FbaI TGATCA 1 cut(s) 571
Fnu4HI GCNGC 1 cut(s) 757
FokI GGATG 4 cut(s) 46, 155, 164, 407
FriOI GRGCYC 1 cut(s) 707
Fsp4HI GCNGC 1 cut(s) 757
FspBI CTAG 1 cut(s) 910
GluI GCNGC 1 cut(s) 757
HaeIII GGCC 4 cut(s) 317, 596, 615, 902
HapII CCGG 3 cut(s) 75, 80, 770
Hin1II CATG 1 cut(s) 859
HincII GTYRAC 1 cut(s) 634
HindII GTYRAC 1 cut(s) 634
HindIII AAGCTT 2 cut(s) 14, 736
HinfI GANTC 5 cut(s) 251, 380, 444, 534, 851
HpaII CCGG 3 cut(s) 75, 80, 770
Hpy166II GTNNAC 1 cut(s) 634
Hpy188I TCNGA 6 cut(s) 91, 142, 256, 493, 582, 702
Hpy188III TCNNGA 2 cut(s) 101, 805
Hpy8I GTNNAC 1 cut(s) 634
HpyAV CCTTC 3 cut(s) 53, 279, 378
HpyCH4III ACNGT 3 cut(s) 338, 670, 779
HpyCH4IV ACGT 2 cut(s) 129, 306
HpyCH4V TGCA 2 cut(s) 498, 650
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 554, 899
HpyF3I CTNAG 5 cut(s) 208, 263, 325, 675, 699
HpySE526I ACGT 2 cut(s) 129, 306
Hsp92II CATG 1 cut(s) 859
Ksp22I TGATCA 1 cut(s) 571
Kzo9I GATC 3 cut(s) 91, 571, 655
LguI GCTCTTC 1 cut(s) 599
LmnI GCTCC 5 cut(s) 113, 218, 562, 710, 753
LweI GCATC 1 cut(s) 426
MaeI CTAG 1 cut(s) 910
MaeII ACGT 2 cut(s) 129, 306
MaeIII GTNAC 1 cut(s) 746
MalI GATC 3 cut(s) 93, 573, 657
MbiI CCGCTC 1 cut(s) 756
MboI GATC 3 cut(s) 91, 571, 655
MboII GAAGA 3 cut(s) 31, 396, 616
MhlI GDGCHC 3 cut(s) 465, 665, 707
MlsI TGGCCA 2 cut(s) 615, 902
MluCI AATT 5 cut(s) 119, 233, 641, 685, 709
MluNI TGGCCA 2 cut(s) 615, 902
MlyI GAGTC 2 cut(s) 245, 543
MmeI TCCRAC 1 cut(s) 279
MnlI CCTC 9 cut(s) 62, 65, 191, 217, 226, 292, 323, 382, 805
Mox20I TGGCCA 2 cut(s) 615, 902
Mph1103I ATGCAT 1 cut(s) 652
MscI TGGCCA 2 cut(s) 615, 902
MseI TTAA 3 cut(s) 18, 309, 786
MslI CAYNNNNRTG 1 cut(s) 668
Msp20I TGGCCA 2 cut(s) 615, 902
MspCI CTTAAG 1 cut(s) 17
MspI CCGG 3 cut(s) 75, 80, 770
MspR9I CCNGG 2 cut(s) 385, 770
MvaI CCWGG 1 cut(s) 385
MwoI GCNNNNNNNGC 3 cut(s) 80, 554, 899
NciI CCSGG 1 cut(s) 770
NdeII GATC 3 cut(s) 91, 571, 655
NlaIII CATG 1 cut(s) 859
NlaIV GGNNCC 4 cut(s) 115, 214, 477, 664
NmuCI GTSAC 1 cut(s) 746
NsiI ATGCAT 1 cut(s) 652
OliI CACNNNNGTG 1 cut(s) 668
PciSI GCTCTTC 1 cut(s) 599
PfeI GAWTC 3 cut(s) 380, 444, 851
PflFI GACNNNGTC 2 cut(s) 338, 629
PflMI CCANNNNNTGG 1 cut(s) 592
PkrI GCNGC 1 cut(s) 758
PleI GAGTC 2 cut(s) 245, 542
PpsI GAGTC 2 cut(s) 245, 542
PsiI TTATAA 1 cut(s) 512
Psp124BI GAGCTC 1 cut(s) 707
Psp1406I AACGTT 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 383
PspGI CCWGG 1 cut(s) 383
PspN4I GGNNCC 4 cut(s) 115, 214, 477, 664
PspPI GGNCC 2 cut(s) 77, 475
PsyI GACNNNGTC 2 cut(s) 338, 629
RsaI GTAC 1 cut(s) 431
RsaNI GTAC 1 cut(s) 430
RseI CAYNNNNRTG 1 cut(s) 668
Rsr2I CGGWCCG 1 cut(s) 77
RsrII CGGWCCG 1 cut(s) 77
SacI GAGCTC 1 cut(s) 707
SapI GCTCTTC 1 cut(s) 599
SaqAI TTAA 3 cut(s) 18, 309, 786
SatI GCNGC 1 cut(s) 757
Sau3AI GATC 3 cut(s) 91, 571, 655
Sau96I GGNCC 2 cut(s) 77, 475
SchI GAGTC 2 cut(s) 245, 543
ScrFI CCNGG 2 cut(s) 385, 770
SduI GDGCHC 3 cut(s) 465, 665, 707
SfaNI GCATC 1 cut(s) 426
SinI GGWCC 2 cut(s) 77, 475
SmiMI CAYNNNNRTG 1 cut(s) 668
SmlI CTYRAG 3 cut(s) 17, 319, 803
SmoI CTYRAG 3 cut(s) 17, 319, 803
Sse9I AATT 5 cut(s) 119, 233, 641, 685, 709
SsiI CCGC 5 cut(s) 473, 720, 756, 759, 871
SspMI CTAG 1 cut(s) 910
SstI GAGCTC 1 cut(s) 707
StyD4I CCNGG 2 cut(s) 383, 768
TaaI ACNGT 3 cut(s) 338, 670, 779
TaiI ACGT 2 cut(s) 132, 309
TaqI TCGA 4 cut(s) 100, 165, 374, 447
TasI AATT 5 cut(s) 119, 233, 641, 685, 709
TatI WGTACW 1 cut(s) 429
TauI GCSGC 1 cut(s) 759
TfiI GAWTC 3 cut(s) 380, 444, 851
Tru1I TTAA 3 cut(s) 18, 309, 786
Tru9I TTAA 3 cut(s) 18, 309, 786
TscAI CASTG 3 cut(s) 343, 352, 751
TseFI GTSAC 1 cut(s) 746
Tsp45I GTSAC 1 cut(s) 746
TspDTI ATGAA 3 cut(s) 166, 670, 872
TspGWI ACGGA 2 cut(s) 456, 549
TspRI CASTG 3 cut(s) 343, 352, 751
Tth111I GACNNNGTC 2 cut(s) 338, 629
Van91I CCANNNNNTGG 1 cut(s) 592
Vha464I CTTAAG 1 cut(s) 17
VpaK11BI GGWCC 2 cut(s) 77, 475
XagI CCTNNNNNAGG 1 cut(s) 796
XapI RAATTY 2 cut(s) 119, 233
XspI CTAG 1 cut(s) 910
Zsp2I ATGCAT 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.