RLG00000025746

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0020678
Physical Location & Seq
Reverse (-)
48035497 .. 48036517
1021 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025746

Sequence Viewer

Length: 609 bp
ATGCGGGCGGACAGCGGGGCGGACGTCGGGGCAGACGCCGAAATGATATGTACCTGCAATTTGTACTCCCAAGTTCCCAGAACTGTGAATCCACGCTCAACACTTAACCAGAGTTTCACCATTTATGGGCTATGGCCTACTAGCAAATATAATGTTGCGATTACATATTCTGCTGATCTTCGCAAAAAGCTGCGGGGAACCGACCTCCCAGATAAACTCCTTCAGTCTTGGCCCAATGTAGTGACTTCAAACAATCCGAATGCAGTTTGGTCCTTCTGGAAATACCAATACAACAAGCACGGCAGCTGCGCCTACTGGAGATTCCAAAGACTGGAGGCTTACTTTAGAAAAGCAGATGACTTGTATCGTAGTGATAGCGTACAATATTTGTATGATTCATTCACCAGCAATCGCCAAGTCTCTGTTATCAGATATATGAATGACGTAAAAAATTATGGAGGGATATTTCCAGGACTGCGGTGCAATGGAACGGGATTCCTGAATGAAGAATCCCGTTCCATTGCACCGCAGTCCTGGAAATTTCCCTCCATAATTTTTTACGTCATTCCTATATCTGATAGCAGAGACTTGGCGGTTGCTGGTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

203

Amino Acids

23.16

Weight (kDa)

8.99

Isoelectric Point (pI)

40.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 19 - 130 6.9e-14 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020678)

Species Orthologous Gene IDs
rosa_laevigata RLG00000025746
rosa_multiflora Rmu_sc0005298.1_g000007 Rmu_sc0010184.1_g000001
rosa_roxburghii Rroxscaffold_6G00427960
rosa_samantha Rh2CG051700 Rh5DG089900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 27
Acc36I ACCTGC 1 cut(s) 62
AccB7I CCANNNNNTGG 1 cut(s) 331
AciI CCGC 8 cut(s) 4, 8, 15, 20, 193, 478, 527, 593
AcsI RAATTY 1 cut(s) 539
AcuI CTGAAG 1 cut(s) 206
AcyI GRCGYC 2 cut(s) 24, 36
AfaI GTAC 3 cut(s) 52, 65, 381
AfiI CCNNNNNNNGG 2 cut(s) 126, 331
AgsI TTSAA 1 cut(s) 249
AjnI CCWGG 2 cut(s) 469, 533
AloI GAACNNNNNNTCC 2 cut(s) 73, 105
AluBI AGCT 2 cut(s) 190, 306
AluI AGCT 2 cut(s) 190, 306
Alw26I GTCTC 2 cut(s) 424, 579
AoxI GGCC 2 cut(s) 134, 230
ApeKI GCWGC 3 cut(s) 190, 303, 306
ApoI RAATTY 1 cut(s) 539
AspLEI GCGC 1 cut(s) 311
AspS9I GGNCC 2 cut(s) 231, 270
AsuHPI GGTGA 2 cut(s) 109, 394
AvaII GGWCC 1 cut(s) 270
BbvI GCAGC 3 cut(s) 177, 293, 315
BceAI ACGGC 1 cut(s) 316
BciT130I CCWGG 2 cut(s) 471, 535
BcoDI GTCTC 2 cut(s) 424, 579
BfaI CTAG 1 cut(s) 141
BfuAI ACCTGC 1 cut(s) 62
BisI GCNGC 3 cut(s) 191, 304, 307
BlsI GCNGC 3 cut(s) 192, 305, 308
Bme1390I CCNGG 2 cut(s) 471, 535
Bme18I GGWCC 1 cut(s) 270
BmgT120I GGNCC 2 cut(s) 231, 270
BmiI GGNNCC 1 cut(s) 199
BmrFI CCNGG 2 cut(s) 471, 535
BpmI CTGGAG 2 cut(s) 337, 353
BsaHI GRCGYC 2 cut(s) 24, 36
Bsc4I CCNNNNNNNGG 2 cut(s) 126, 331
Bse1I ACTGG 2 cut(s) 320, 336
Bse3DI GCAATG 2 cut(s) 490, 519
BseBI CCWGG 2 cut(s) 471, 535
BseLI CCNNNNNNNGG 2 cut(s) 126, 331
BseMI GCAATG 2 cut(s) 490, 519
BseNI ACTGG 2 cut(s) 320, 336
BseXI GCAGC 3 cut(s) 177, 293, 315
BshFI GGCC 2 cut(s) 136, 232
BslI CCNNNNNNNGG 2 cut(s) 126, 331
BsmAI GTCTC 2 cut(s) 424, 579
BsmI GAATGC 1 cut(s) 265
BsnI GGCC 2 cut(s) 136, 232
Bsp143I GATC 1 cut(s) 175
BspACI CCGC 8 cut(s) 4, 8, 15, 20, 193, 478, 527, 593
BspANI GGCC 2 cut(s) 136, 232
BspLI GGNNCC 1 cut(s) 199
BspMI ACCTGC 1 cut(s) 62
BsrDI GCAATG 2 cut(s) 490, 519
BsrI ACTGG 2 cut(s) 320, 336
BssMI GATC 1 cut(s) 175
BssNI GRCGYC 2 cut(s) 24, 36
Bst2UI CCWGG 2 cut(s) 471, 535
Bst4CI ACNGT 1 cut(s) 85
BstACI GRCGYC 2 cut(s) 24, 36
BstC8I GCNNGC 1 cut(s) 6
BstHHI GCGC 1 cut(s) 311
BstKTI GATC 1 cut(s) 178
BstMAI GTCTC 2 cut(s) 424, 579
BstMBI GATC 1 cut(s) 175
BstNI CCWGG 2 cut(s) 471, 535
BstSCI CCNGG 2 cut(s) 469, 533
BstV1I GCAGC 3 cut(s) 177, 293, 315
BsuRI GGCC 2 cut(s) 136, 232
BveI ACCTGC 1 cut(s) 62
Cac8I GCNNGC 1 cut(s) 6
CfoI GCGC 1 cut(s) 311
Cfr13I GGNCC 2 cut(s) 231, 270
CseI GACGC 1 cut(s) 44
Csp6I GTAC 3 cut(s) 51, 64, 380
CviJI RGCY 6 cut(s) 130, 136, 190, 232, 306, 338
CviKI_1 RGCY 6 cut(s) 130, 136, 190, 232, 306, 338
CviQI GTAC 3 cut(s) 51, 64, 380
DpnI GATC 1 cut(s) 177
DpnII GATC 1 cut(s) 175
EciI GGCGGA 2 cut(s) 23, 35
Eco47I GGWCC 1 cut(s) 270
Eco57I CTGAAG 1 cut(s) 206
EcoRII CCWGG 2 cut(s) 469, 533
FauI CCCGC 2 cut(s) 8, 186
Fnu4HI GCNGC 3 cut(s) 191, 304, 307
Fsp4HI GCNGC 3 cut(s) 191, 304, 307
FspBI CTAG 1 cut(s) 141
GlaI GCGC 1 cut(s) 310
GluI GCNGC 3 cut(s) 191, 304, 307
GsuI CTGGAG 2 cut(s) 337, 353
HaeIII GGCC 2 cut(s) 136, 232
HgaI GACGC 1 cut(s) 44
HhaI GCGC 1 cut(s) 311
Hin1I GRCGYC 2 cut(s) 24, 36
Hin6I GCGC 1 cut(s) 309
HinP1I GCGC 1 cut(s) 309
HinfI GANTC 5 cut(s) 88, 321, 395, 495, 509
HphI GGTGA 2 cut(s) 109, 394
Hpy188I TCNGA 3 cut(s) 258, 431, 577
Hpy188III TCNNGA 2 cut(s) 277, 499
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 2 cut(s) 230, 283
HpyCH4III ACNGT 1 cut(s) 85
HpyCH4IV ACGT 3 cut(s) 24, 444, 561
HpyCH4V TGCA 4 cut(s) 57, 263, 483, 524
HpySE526I ACGT 3 cut(s) 24, 444, 561
Hsp92I GRCGYC 2 cut(s) 24, 36
HspAI GCGC 1 cut(s) 309
Kzo9I GATC 1 cut(s) 175
Lsp1109I GCAGC 3 cut(s) 177, 293, 315
MaeI CTAG 1 cut(s) 141
MaeII ACGT 3 cut(s) 24, 444, 561
MaeIII GTNAC 1 cut(s) 241
MalI GATC 1 cut(s) 177
MboI GATC 1 cut(s) 175
MboII GAAGA 2 cut(s) 170, 518
MluCI AATT 4 cut(s) 58, 451, 539, 552
MnlI CCTC 4 cut(s) 215, 328, 452, 556
MseI TTAA 1 cut(s) 105
MspA1I CMGCKG 2 cut(s) 15, 306
MspR9I CCNGG 2 cut(s) 471, 535
Mva1269I GAATGC 1 cut(s) 265
MvaI CCWGG 2 cut(s) 471, 535
NdeII GATC 1 cut(s) 175
NlaIV GGNNCC 1 cut(s) 199
NmuCI GTSAC 1 cut(s) 241
PctI GAATGC 1 cut(s) 265
PfeI GAWTC 5 cut(s) 88, 321, 395, 495, 509
PflMI CCANNNNNTGG 1 cut(s) 331
PfoI TCCNGGA 2 cut(s) 469, 533
PkrI GCNGC 3 cut(s) 192, 305, 308
Psp6I CCWGG 2 cut(s) 469, 533
PspGI CCWGG 2 cut(s) 469, 533
PspN4I GGNNCC 1 cut(s) 199
PspPI GGNCC 2 cut(s) 231, 270
PvuII CAGCTG 1 cut(s) 306
RsaI GTAC 3 cut(s) 52, 65, 381
RsaNI GTAC 3 cut(s) 51, 64, 380
SaqAI TTAA 1 cut(s) 105
SatI GCNGC 3 cut(s) 191, 304, 307
Sau3AI GATC 1 cut(s) 175
Sau96I GGNCC 2 cut(s) 231, 270
ScrFI CCNGG 2 cut(s) 471, 535
SetI ASST 7 cut(s) 27, 56, 192, 207, 308, 447, 564
SinI GGWCC 1 cut(s) 270
Sse9I AATT 4 cut(s) 58, 451, 539, 552
SsiI CCGC 8 cut(s) 4, 8, 15, 20, 193, 478, 527, 593
SspI AATATT 1 cut(s) 386
SspMI CTAG 1 cut(s) 141
StyD4I CCNGG 2 cut(s) 469, 533
TaaI ACNGT 1 cut(s) 85
TaiI ACGT 3 cut(s) 27, 447, 564
TasI AATT 4 cut(s) 58, 451, 539, 552
TatI WGTACW 1 cut(s) 63
TfiI GAWTC 5 cut(s) 88, 321, 395, 495, 509
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TseFI GTSAC 1 cut(s) 241
TseI GCWGC 3 cut(s) 190, 303, 306
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 3 cut(s) 387, 452, 519
Van91I CCANNNNNTGG 1 cut(s) 331
VpaK11BI GGWCC 1 cut(s) 270
XapI RAATTY 1 cut(s) 539
XspI CTAG 1 cut(s) 141
ZraI GACGTC 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.