Rroxscaffold_6G00427960

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0020678
Physical Location & Seq
Reverse (-)
48487217 .. 48491623
4407 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00427960.1

Sequence Viewer

Length: 546 bp
ATGGCGGCAAAACATTTTCACCTGCAACTGAGCAACGAACTCCAAACGGATCTCAGTACGTCTTGGCCCGATGTAATGAACGGAAACAATTCGCGGTTTTGGACCGACGAATACAATAAGCACGGCACCTGCGCCAGCCATACATTCACAACGCCGCAGGCTTACTTTGAGAAAGCAGATTACTTGTATCGCAGCTTCGCTGGCGGACAAAATTTGTATGATTTATTTGTCCGCGGCGGCATCCGCCCGGGACAGCAAAACGACACTATGATATATATGAATCTCGTAAAAAATGCGACTGGAGAGTTTCCCCGACTGTGGTGCGGTGGAAAAAAACTGAAAGAAGTAGTCATTTGTTACAATAAAGATGCAACTCACGTGATTGATTGTCCTCAATACAAAAGGAAGATTGATTGTACGGGGCTCATATTAATACGAACAGGAAGAGCCCTTTATGGTCCAACCATATTGGTATTGTCCTTTAGACCCTTGGGGCTAATTATCTTTAAATATGGTCCAAGCTATATTAAATCTAAGCTAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

181

Amino Acids

20.63

Weight (kDa)

9.27

Isoelectric Point (pI)

27.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 5 - 130 3.3e-20 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020678)

Species Orthologous Gene IDs
rosa_laevigata RLG00000025746
rosa_multiflora Rmu_sc0005298.1_g000007 Rmu_sc0010184.1_g000001
rosa_roxburghii Rroxscaffold_6G00427960
rosa_samantha Rh2CG051700 Rh5DG089900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 30, 137
Acc36I ACCTGC 2 cut(s) 30, 137
AccB1I GGYRCC 1 cut(s) 125
AccII CGCG 2 cut(s) 94, 234
AciI CCGC 9 cut(s) 5, 94, 155, 204, 232, 234, 237, 244, 324
AclWI GGATC 1 cut(s) 57
AcsI RAATTY 1 cut(s) 211
AcvI CACGTG 1 cut(s) 379
AfaI GTAC 2 cut(s) 58, 418
AfiI CCNNNNNNNGG 1 cut(s) 318
AluBI AGCT 4 cut(s) 195, 522, 538, 542
AluI AGCT 4 cut(s) 195, 522, 538, 542
AlwI GGATC 1 cut(s) 57
Ama87I CYCGRG 1 cut(s) 247
AoxI GGCC 1 cut(s) 65
ApeKI GCWGC 1 cut(s) 192
ApoI RAATTY 1 cut(s) 211
AseI ATTAAT 1 cut(s) 431
Asp700I GAANNNNTTC 1 cut(s) 88
AspLEI GCGC 1 cut(s) 134
AspS9I GGNCC 4 cut(s) 66, 102, 458, 515
AsuC2I CCSGG 2 cut(s) 248, 249
AsuHPI GGTGA 1 cut(s) 11
AsuNHI GCTAGC 1 cut(s) 538
AvaI CYCGRG 1 cut(s) 247
AvaII GGWCC 3 cut(s) 102, 458, 515
BanI GGYRCC 1 cut(s) 125
BanII GRGCYC 2 cut(s) 426, 451
BarI GAAGNNNNNNTAC 2 cut(s) 179, 211
BbrPI CACGTG 1 cut(s) 379
BbvI GCAGC 1 cut(s) 204
BceAI ACGGC 1 cut(s) 139
BcgI CGANNNNNNTGC 2 cut(s) 303, 337
BcnI CCSGG 2 cut(s) 248, 249
BfaI CTAG 1 cut(s) 539
BfuAI ACCTGC 2 cut(s) 30, 137
BisI GCNGC 5 cut(s) 6, 155, 193, 235, 238
BlsI GCNGC 5 cut(s) 7, 156, 194, 236, 239
Bme1390I CCNGG 2 cut(s) 248, 249
Bme18I GGWCC 3 cut(s) 102, 458, 515
BmeT110I CYCGRG 1 cut(s) 247
BmgT120I GGNCC 4 cut(s) 66, 102, 458, 515
BmiI GGNNCC 1 cut(s) 127
BmrFI CCNGG 2 cut(s) 248, 249
BmsI GCATC 2 cut(s) 249, 358
BmtI GCTAGC 1 cut(s) 542
BpmI CTGGAG 1 cut(s) 321
BpuMI CCSGG 2 cut(s) 248, 249
BsaAI YACGTR 1 cut(s) 379
BsaJI CCNNGG 3 cut(s) 232, 247, 489
Bsc4I CCNNNNNNNGG 1 cut(s) 318
Bse1I ACTGG 1 cut(s) 304
BseDI CCNNGG 3 cut(s) 232, 247, 489
BseGI GGATG 1 cut(s) 240
BseLI CCNNNNNNNGG 1 cut(s) 318
BseMII CTCAG 2 cut(s) 20, 67
BseNI ACTGG 1 cut(s) 304
BseXI GCAGC 1 cut(s) 204
Bsh1236I CGCG 2 cut(s) 94, 234
BshFI GGCC 1 cut(s) 67
BshNI GGYRCC 1 cut(s) 125
BsiHKCI CYCGRG 1 cut(s) 247
BsiSI CCGG 1 cut(s) 248
BslFI GGGAC 1 cut(s) 264
BslI CCNNNNNNNGG 1 cut(s) 318
BsmFI GGGAC 1 cut(s) 264
BsnI GGCC 1 cut(s) 67
BsoBI CYCGRG 1 cut(s) 247
Bsp1286I GDGCHC 2 cut(s) 426, 451
Bsp143I GATC 1 cut(s) 49
BspACI CCGC 9 cut(s) 5, 94, 155, 204, 232, 234, 237, 244, 324
BspANI GGCC 1 cut(s) 67
BspCNI CTCAG 2 cut(s) 21, 66
BspFNI CGCG 2 cut(s) 94, 234
BspLI GGNNCC 1 cut(s) 127
BspMI ACCTGC 2 cut(s) 30, 137
BspOI GCTAGC 1 cut(s) 542
BspPI GGATC 1 cut(s) 57
BspQI GCTCTTC 1 cut(s) 439
BspT107I GGYRCC 1 cut(s) 125
BsrI ACTGG 1 cut(s) 304
BssECI CCNNGG 3 cut(s) 232, 247, 489
BssMI GATC 1 cut(s) 49
BssT1I CCWWGG 1 cut(s) 489
Bst4CI ACNGT 1 cut(s) 318
Bst6I CTCTTC 1 cut(s) 439
BstBAI YACGTR 1 cut(s) 379
BstC8I GCNNGC 4 cut(s) 136, 159, 202, 540
BstDEI CTNAG 3 cut(s) 29, 53, 534
BstDSI CCRYGG 1 cut(s) 232
BstF5I GGATG 1 cut(s) 240
BstFNI CGCG 2 cut(s) 94, 234
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 1 cut(s) 52
BstMBI GATC 1 cut(s) 49
BstMWI GCNNNNNNNGC 2 cut(s) 201, 243
BstSCI CCNGG 2 cut(s) 246, 247
BstUI CGCG 2 cut(s) 94, 234
BstV1I GCAGC 1 cut(s) 204
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
BsuRI GGCC 1 cut(s) 67
BtgI CCRYGG 1 cut(s) 232
BtsCI GGATG 1 cut(s) 240
BveI ACCTGC 2 cut(s) 30, 137
Cac8I GCNNGC 4 cut(s) 136, 159, 202, 540
CfoI GCGC 1 cut(s) 134
Cfr13I GGNCC 4 cut(s) 66, 102, 458, 515
Cfr42I CCGCGG 1 cut(s) 235
Cfr9I CCCGGG 1 cut(s) 247
Csp6I GTAC 2 cut(s) 57, 417
CviQI GTAC 2 cut(s) 57, 417
DdeI CTNAG 3 cut(s) 29, 53, 534
DpnI GATC 1 cut(s) 51
DpnII GATC 1 cut(s) 49
DraI TTTAAA 1 cut(s) 508
Eam1104I CTCTTC 1 cut(s) 439
EarI CTCTTC 1 cut(s) 439
EciI GGCGGA 2 cut(s) 219, 233
Eco130I CCWWGG 1 cut(s) 489
Eco24I GRGCYC 2 cut(s) 426, 451
Eco47I GGWCC 3 cut(s) 102, 458, 515
Eco72I CACGTG 1 cut(s) 379
Eco88I CYCGRG 1 cut(s) 247
EcoT14I CCWWGG 1 cut(s) 489
EcoT38I GRGCYC 2 cut(s) 426, 451
ErhI CCWWGG 1 cut(s) 489
FaqI GGGAC 1 cut(s) 264
Fnu4HI GCNGC 5 cut(s) 6, 155, 193, 235, 238
FokI GGATG 1 cut(s) 227
FriOI GRGCYC 2 cut(s) 426, 451
Fsp4HI GCNGC 5 cut(s) 6, 155, 193, 235, 238
FspBI CTAG 1 cut(s) 539
GlaI GCGC 1 cut(s) 133
GluI GCNGC 5 cut(s) 6, 155, 193, 235, 238
GsuI CTGGAG 1 cut(s) 321
HaeIII GGCC 1 cut(s) 67
HapII CCGG 1 cut(s) 248
HhaI GCGC 1 cut(s) 134
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HinfI GANTC 1 cut(s) 280
HpaII CCGG 1 cut(s) 248
HphI GGTGA 1 cut(s) 11
Hpy99I CGWCG 1 cut(s) 110
HpyCH4III ACNGT 1 cut(s) 318
HpyCH4IV ACGT 2 cut(s) 59, 378
HpyCH4V TGCA 2 cut(s) 25, 371
HpyF10VI GCNNNNNNNGC 2 cut(s) 201, 243
HpyF3I CTNAG 3 cut(s) 29, 53, 534
HpySE526I ACGT 2 cut(s) 59, 378
HspAI GCGC 1 cut(s) 132
KspI CCGCGG 1 cut(s) 235
Kzo9I GATC 1 cut(s) 49
LguI GCTCTTC 1 cut(s) 439
LpnPI CCDG 8 cut(s) 35, 142, 143, 148, 186, 261, 285, 426
Lsp1109I GCAGC 1 cut(s) 204
LweI GCATC 2 cut(s) 249, 358
MaeI CTAG 1 cut(s) 539
MaeII ACGT 2 cut(s) 59, 378
MaeIII GTNAC 1 cut(s) 356
MalI GATC 1 cut(s) 51
MboI GATC 1 cut(s) 49
MboII GAAGA 2 cut(s) 418, 456
MflI RGATCY 1 cut(s) 49
MhlI GDGCHC 2 cut(s) 426, 451
MluCI AATT 3 cut(s) 88, 211, 498
MmeI TCCRAC 1 cut(s) 485
MnlI CCTC 1 cut(s) 402
MroXI GAANNNNTTC 1 cut(s) 88
MseI TTAA 3 cut(s) 431, 507, 528
MspA1I CMGCKG 1 cut(s) 234
MspI CCGG 1 cut(s) 248
MspR9I CCNGG 2 cut(s) 248, 249
MvnI CGCG 2 cut(s) 94, 234
MwoI GCNNNNNNNGC 2 cut(s) 201, 243
NciI CCSGG 2 cut(s) 248, 249
NdeII GATC 1 cut(s) 49
NheI GCTAGC 1 cut(s) 538
NlaIV GGNNCC 1 cut(s) 127
PaqCI CACCTGC 2 cut(s) 30, 137
PciSI GCTCTTC 1 cut(s) 439
PdmI GAANNNNTTC 1 cut(s) 88
PfeI GAWTC 1 cut(s) 280
PkrI GCNGC 5 cut(s) 7, 156, 194, 236, 239
PmaCI CACGTG 1 cut(s) 379
PmlI CACGTG 1 cut(s) 379
Ppu21I YACGTR 1 cut(s) 379
PshBI ATTAAT 1 cut(s) 431
PspCI CACGTG 1 cut(s) 379
PspN4I GGNNCC 1 cut(s) 127
PspPI GGNCC 4 cut(s) 66, 102, 458, 515
PsuI RGATCY 1 cut(s) 49
RsaI GTAC 2 cut(s) 58, 418
RsaNI GTAC 2 cut(s) 57, 417
SacII CCGCGG 1 cut(s) 235
SapI GCTCTTC 1 cut(s) 439
SaqAI TTAA 3 cut(s) 431, 507, 528
SatI GCNGC 5 cut(s) 6, 155, 193, 235, 238
Sau3AI GATC 1 cut(s) 49
Sau96I GGNCC 4 cut(s) 66, 102, 458, 515
ScrFI CCNGG 2 cut(s) 248, 249
SduI GDGCHC 2 cut(s) 426, 451
SetI ASST 8 cut(s) 24, 62, 131, 197, 381, 524, 540, 544
SfaNI GCATC 2 cut(s) 249, 358
Sfr303I CCGCGG 1 cut(s) 235
SgrBI CCGCGG 1 cut(s) 235
SinI GGWCC 3 cut(s) 102, 458, 515
SmaI CCCGGG 1 cut(s) 249
Sse9I AATT 3 cut(s) 88, 211, 498
SsiI CCGC 9 cut(s) 5, 94, 155, 204, 232, 234, 237, 244, 324
SspMI CTAG 1 cut(s) 539
StyD4I CCNGG 2 cut(s) 246, 247
StyI CCWWGG 1 cut(s) 489
TaaI ACNGT 1 cut(s) 318
TaiI ACGT 2 cut(s) 62, 381
TaqII GACCGA 1 cut(s) 119
TasI AATT 3 cut(s) 88, 211, 498
TauI GCSGC 4 cut(s) 8, 157, 237, 240
TfiI GAWTC 1 cut(s) 280
Tru1I TTAA 3 cut(s) 431, 507, 528
Tru9I TTAA 3 cut(s) 431, 507, 528
TseI GCWGC 1 cut(s) 192
TspDTI ATGAA 2 cut(s) 92, 293
TspGWI ACGGA 2 cut(s) 62, 96
TspMI CCCGGG 1 cut(s) 247
VpaK11BI GGWCC 3 cut(s) 102, 458, 515
VspI ATTAAT 1 cut(s) 431
XapI RAATTY 1 cut(s) 211
XmaI CCCGGG 1 cut(s) 247
XmnI GAANNNNTTC 1 cut(s) 88
XspI CTAG 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.