Rmu_sc0010184.1_g000001

ribonuclease T2 activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010184.1
OG0020678
Physical Location & Seq
Forward (+)
2083 .. 2855
773 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010184.1_g000001.1.cds

Sequence Viewer

Length: 576 bp
atgggtggagcaacagtggcatcagtcattgtgctatttttccttccactcggtgtgattggaaatgcggtcggacagccgaatcacaattatgactacatgcaatttgtgcagcaatatcccaaaacattgaatgtaagctcagaatataccgagcgtttcaccattcatggcctatggcctagcaattccttcggctacggttctgatctacattgtcaaacaccatatgctaaccgttatcaactttcaggacagaagcacggcagctgcgctgtggcccttctgggtatattcccaacagcgcaggcttactttgaaagagcacataagttgtgggaagcaaatgatttgtatggtgtattcgtcaactacaacatccacccgggagaccgagtcgacgctagcaaatttaggacagccctgagaattgcgactggatggaacgctcgactgtcatgcactggaaccggcctaatgatggaagtagttatttgttatgatattcatgcaactcgagtgaggaattgtcctagaccggggaattgtacggggcttatatcatatccaaaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

191

Amino Acids

21.23

Weight (kDa)

8.74

Isoelectric Point (pI)

34.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020678)

Species Orthologous Gene IDs
rosa_laevigata RLG00000025746
rosa_multiflora Rmu_sc0005298.1_g000007 Rmu_sc0010184.1_g000001
rosa_roxburghii Rroxscaffold_6G00427960
rosa_samantha Rh2CG051700 Rh5DG089900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 399
AciI CCGC 1 cut(s) 68
AcsI RAATTY 1 cut(s) 410
AdeI CACNNNGTG 1 cut(s) 53
AfaI GTAC 1 cut(s) 550
AfiI CCNNNNNNNGG 2 cut(s) 481, 539
AgsI TTSAA 2 cut(s) 133, 320
AluBI AGCT 2 cut(s) 141, 270
AluI AGCT 2 cut(s) 141, 270
Alw21I GWGCWC 1 cut(s) 328
Alw26I GTCTC 1 cut(s) 384
Ama87I CYCGRG 2 cut(s) 385, 516
AoxI GGCC 4 cut(s) 172, 179, 279, 472
ApeKI GCWGC 3 cut(s) 112, 267, 270
ApoI RAATTY 1 cut(s) 410
AspLEI GCGC 2 cut(s) 275, 307
AspS9I GGNCC 1 cut(s) 280
AsuC2I CCSGG 3 cut(s) 386, 387, 540
AsuHPI GGTGA 1 cut(s) 154
AsuNHI GCTAGC 1 cut(s) 404
AvaI CYCGRG 2 cut(s) 385, 516
Bbv12I GWGCWC 1 cut(s) 328
BbvI GCAGC 3 cut(s) 124, 257, 279
BccI CCATC 2 cut(s) 435, 475
BceAI ACGGC 1 cut(s) 280
BcgI CGANNNNNNTGC 4 cut(s) 175, 209, 441, 475
BcnI CCSGG 3 cut(s) 386, 387, 540
BcoDI GTCTC 1 cut(s) 384
BfaI CTAG 3 cut(s) 183, 405, 534
BisI GCNGC 3 cut(s) 113, 268, 271
BlsI GCNGC 3 cut(s) 114, 269, 272
Bme1390I CCNGG 3 cut(s) 386, 387, 540
BmeT110I CYCGRG 2 cut(s) 385, 516
BmgT120I GGNCC 1 cut(s) 280
BmiI GGNNCC 1 cut(s) 469
BmrFI CCNGG 3 cut(s) 386, 387, 540
BmsI GCATC 1 cut(s) 29
BmtI GCTAGC 1 cut(s) 408
BpuMI CCSGG 3 cut(s) 386, 387, 540
BsaI GGTCTC 1 cut(s) 384
BsaJI CCNNGG 2 cut(s) 385, 539
BsaXI ACNNNNNCTCC 3 cut(s) 30, 381, 411
Bsc4I CCNNNNNNNGG 2 cut(s) 481, 539
Bse118I RCCGGY 1 cut(s) 470
Bse1I ACTGG 2 cut(s) 442, 469
BseDI CCNNGG 2 cut(s) 385, 539
BseGI GGATG 2 cut(s) 378, 446
BseLI CCNNNNNNNGG 2 cut(s) 481, 539
BseMII CTCAG 2 cut(s) 156, 416
BseNI ACTGG 2 cut(s) 442, 469
BseXI GCAGC 3 cut(s) 124, 257, 279
BsgI GTGCAG 1 cut(s) 131
Bsh1285I CGRYCG 1 cut(s) 72
BshFI GGCC 4 cut(s) 174, 181, 281, 474
BsiEI CGRYCG 1 cut(s) 72
BsiHKAI GWGCWC 1 cut(s) 328
BsiHKCI CYCGRG 2 cut(s) 385, 516
BsiSI CCGG 3 cut(s) 386, 471, 539
BslI CCNNNNNNNGG 2 cut(s) 481, 539
BsmAI GTCTC 1 cut(s) 384
BsnI GGCC 4 cut(s) 174, 181, 281, 474
Bso31I GGTCTC 1 cut(s) 384
BsoBI CYCGRG 2 cut(s) 385, 516
Bsp1286I GDGCHC 1 cut(s) 328
Bsp143I GATC 1 cut(s) 208
BspACI CCGC 1 cut(s) 68
BspANI GGCC 4 cut(s) 174, 181, 281, 474
BspCNI CTCAG 2 cut(s) 155, 417
BspLI GGNNCC 1 cut(s) 469
BspOI GCTAGC 1 cut(s) 408
BspTNI GGTCTC 1 cut(s) 384
BsrFI RCCGGY 1 cut(s) 470
BsrI ACTGG 2 cut(s) 442, 469
BssAI RCCGGY 1 cut(s) 470
BssECI CCNNGG 2 cut(s) 385, 539
BssMI GATC 1 cut(s) 208
Bst4CI ACNGT 4 cut(s) 16, 203, 239, 456
BstAPI GCANNNNNTGC 1 cut(s) 109
BstC8I GCNNGC 2 cut(s) 309, 406
BstDEI CTNAG 2 cut(s) 142, 425
BstF5I GGATG 2 cut(s) 378, 446
BstHHI GCGC 2 cut(s) 275, 307
BstKTI GATC 1 cut(s) 211
BstMAI GTCTC 1 cut(s) 384
BstMBI GATC 1 cut(s) 208
BstMCI CGRYCG 1 cut(s) 72
BstMWI GCNNNNNNNGC 2 cut(s) 17, 109
BstNSI RCATGY 1 cut(s) 103
BstSCI CCNGG 3 cut(s) 384, 385, 538
BstV1I GCAGC 3 cut(s) 124, 257, 279
BsuRI GGCC 4 cut(s) 174, 181, 281, 474
BtsCI GGATG 2 cut(s) 378, 446
BtsIMutI CAGTG 2 cut(s) 21, 462
Cac8I GCNNGC 2 cut(s) 309, 406
CfoI GCGC 2 cut(s) 275, 307
Cfr10I RCCGGY 1 cut(s) 470
Cfr13I GGNCC 1 cut(s) 280
Cfr9I CCCGGG 1 cut(s) 385
CseI GACGC 1 cut(s) 410
Csp6I GTAC 1 cut(s) 549
CviAII CATG 4 cut(s) 100, 170, 459, 509
CviQI GTAC 1 cut(s) 549
DdeI CTNAG 2 cut(s) 142, 425
DpnI GATC 1 cut(s) 210
DpnII GATC 1 cut(s) 208
DraIII CACNNNGTG 1 cut(s) 53
Eco31I GGTCTC 1 cut(s) 384
Eco88I CYCGRG 2 cut(s) 385, 516
FaeI CATG 4 cut(s) 103, 173, 462, 512
FatI CATG 4 cut(s) 99, 169, 458, 508
FauNDI CATATG 1 cut(s) 229
FblI GTMKAC 1 cut(s) 399
Fnu4HI GCNGC 3 cut(s) 113, 268, 271
FokI GGATG 2 cut(s) 365, 453
Fsp4HI GCNGC 3 cut(s) 113, 268, 271
FspBI CTAG 3 cut(s) 183, 405, 534
GlaI GCGC 2 cut(s) 274, 306
GluI GCNGC 3 cut(s) 113, 268, 271
HaeIII GGCC 4 cut(s) 174, 181, 281, 474
HapII CCGG 3 cut(s) 386, 471, 539
HgaI GACGC 1 cut(s) 410
HhaI GCGC 2 cut(s) 275, 307
Hin1II CATG 4 cut(s) 103, 173, 462, 512
Hin6I GCGC 2 cut(s) 273, 305
HinP1I GCGC 2 cut(s) 273, 305
HincII GTYRAC 2 cut(s) 370, 400
HindII GTYRAC 2 cut(s) 370, 400
HinfI GANTC 2 cut(s) 82, 396
HpaII CCGG 3 cut(s) 386, 471, 539
HphI GGTGA 1 cut(s) 154
Hpy166II GTNNAC 2 cut(s) 370, 400
Hpy188I TCNGA 3 cut(s) 74, 145, 208
Hpy188III TCNNGA 1 cut(s) 252
Hpy8I GTNNAC 2 cut(s) 370, 400
Hpy99I CGWCG 1 cut(s) 404
HpyAV CCTTC 3 cut(s) 53, 202, 293
HpyCH4III ACNGT 4 cut(s) 16, 203, 239, 456
HpyCH4V TGCA 4 cut(s) 103, 112, 462, 512
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 109
HpyF3I CTNAG 2 cut(s) 142, 425
Hsp92II CATG 4 cut(s) 103, 173, 462, 512
HspAI GCGC 2 cut(s) 273, 305
Kzo9I GATC 1 cut(s) 208
LmnI GCTCC 1 cut(s) 8
LpnPI CCDG 9 cut(s) 237, 272, 293, 399, 423, 437, 450, 484, 552
Lsp1109I GCAGC 3 cut(s) 124, 257, 279
LweI GCATC 1 cut(s) 29
MaeI CTAG 3 cut(s) 183, 405, 534
MalI GATC 1 cut(s) 210
MboI GATC 1 cut(s) 208
MhlI GDGCHC 1 cut(s) 328
MluCI AATT 7 cut(s) 88, 104, 187, 410, 429, 526, 544
MlyI GAGTC 1 cut(s) 405
MmeI TCCRAC 1 cut(s) 52
MnlI CCTC 1 cut(s) 516
MslI CAYNNNNRTG 1 cut(s) 90
MspA1I CMGCKG 1 cut(s) 270
MspI CCGG 3 cut(s) 386, 471, 539
MspR9I CCNGG 3 cut(s) 386, 387, 540
MwoI GCNNNNNNNGC 2 cut(s) 17, 109
NciI CCSGG 3 cut(s) 386, 387, 540
NdeI CATATG 1 cut(s) 229
NdeII GATC 1 cut(s) 208
NheI GCTAGC 1 cut(s) 404
NlaIII CATG 4 cut(s) 103, 173, 462, 512
NlaIV GGNNCC 1 cut(s) 469
NspI RCATGY 1 cut(s) 103
PaeR7I CTCGAG 1 cut(s) 516
PfeI GAWTC 1 cut(s) 82
PflFI GACNNNGTC 1 cut(s) 395
PkrI GCNGC 3 cut(s) 114, 269, 272
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
PspN4I GGNNCC 1 cut(s) 469
PspPI GGNCC 1 cut(s) 280
PspXI VCTCGAGB 1 cut(s) 516
PsyI GACNNNGTC 1 cut(s) 395
PvuII CAGCTG 1 cut(s) 270
RsaI GTAC 1 cut(s) 550
RsaNI GTAC 1 cut(s) 549
RseI CAYNNNNRTG 1 cut(s) 90
SalI GTCGAC 1 cut(s) 398
SatI GCNGC 3 cut(s) 113, 268, 271
Sau3AI GATC 1 cut(s) 208
Sau96I GGNCC 1 cut(s) 280
SchI GAGTC 1 cut(s) 405
ScrFI CCNGG 3 cut(s) 386, 387, 540
SduI GDGCHC 1 cut(s) 328
SetI ASST 2 cut(s) 143, 272
SfaNI GCATC 1 cut(s) 29
Sfr274I CTCGAG 1 cut(s) 516
SlaI CTCGAG 1 cut(s) 516
SmaI CCCGGG 1 cut(s) 387
SmiMI CAYNNNNRTG 1 cut(s) 90
SmlI CTYRAG 1 cut(s) 516
SmoI CTYRAG 1 cut(s) 516
Sse9I AATT 7 cut(s) 88, 104, 187, 410, 429, 526, 544
SsiI CCGC 1 cut(s) 68
SspMI CTAG 3 cut(s) 183, 405, 534
StyD4I CCNGG 3 cut(s) 384, 385, 538
TaaI ACNGT 4 cut(s) 16, 203, 239, 456
TaqI TCGA 3 cut(s) 399, 451, 517
TaqII GACCGA 1 cut(s) 408
TasI AATT 7 cut(s) 88, 104, 187, 410, 429, 526, 544
TfiI GAWTC 1 cut(s) 82
TscAI CASTG 2 cut(s) 21, 469
TseI GCWGC 3 cut(s) 112, 267, 270
TspDTI ATGAA 2 cut(s) 158, 497
TspMI CCCGGG 1 cut(s) 385
TspRI CASTG 2 cut(s) 21, 469
Tth111I GACNNNGTC 1 cut(s) 395
XapI RAATTY 1 cut(s) 410
XceI RCATGY 1 cut(s) 103
XhoI CTCGAG 1 cut(s) 516
XmaI CCCGGG 1 cut(s) 385
XmiI GTMKAC 1 cut(s) 399
XspI CTAG 3 cut(s) 183, 405, 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.