RLG00000027030

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0020035
Physical Location & Seq
Reverse (-)
7796454 .. 7797861
1408 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027030

Sequence Viewer

Length: 456 bp
ATGGTTTTGAAGTTGTCCGTGAGGTTTCTCAAATTTGCAGCTGATGTTGAGGTGACGATGGTGAAGGTGGAAGTCTATAAGTCTGATGATGAGACGACAACGTTCGATGGGCACAATATGTTGGACGACGAACATGATCATACTATAGATGTTATTTCAGTGCCGAAGCAACCATCAACTATGGGGGACTCTATCACGGCGCTGGTGTCCATCCGTGGGAGTAAGAATATCTCACAAAGAGACTATAAAGCAACTCAAAAGTCAAGCACAATTGTACCCGACTCCTACCGAAAGAAGGGAGAACCGGTTCGTCATGAGGAACAGATGCGGAAGCCAGAGAAGCAGGTGTGGTCCTGGCTGAAAACGAGGTGGAGGAGTGTGGTGGGGATTGGGTCAGGTTTCGGCCTGGGACGGGTCGGGTTCGAAGTCGGAGGCCGATTTGGGTGGAGTGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

152

Amino Acids

17.09

Weight (kDa)

8.83

Isoelectric Point (pI)

34.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020035)

Species Orthologous Gene IDs
rosa_laevigata RLG00000027030
rosa_multiflora Rmu_sc0002558.1_g000002
rosa_roxburghii Rroxscaffold_4G00286490 Rroxscaffold_4G00286500
rosa_rugosa Rorug01G0357400
rosa_samantha Rh1CG343800 Rh1DG361100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 334
Acc36I ACCTGC 1 cut(s) 334
AciI CCGC 1 cut(s) 328
AclI AACGTT 1 cut(s) 101
AcsI RAATTY 1 cut(s) 32
AfaI GTAC 1 cut(s) 276
AfiI CCNNNNNNNGG 3 cut(s) 216, 295, 412
AgeI ACCGGT 1 cut(s) 304
AgsI TTSAA 1 cut(s) 10
AjnI CCWGG 2 cut(s) 353, 405
AloI GAACNNNNNNTCC 2 cut(s) 291, 323
AluBI AGCT 1 cut(s) 41
AluI AGCT 1 cut(s) 41
Alw26I GTCTC 2 cut(s) 86, 234
AoxI GGCC 2 cut(s) 403, 433
ApeKI GCWGC 1 cut(s) 38
ApoI RAATTY 1 cut(s) 32
AsiGI ACCGGT 1 cut(s) 304
Asp700I GAANNNNTTC 1 cut(s) 306
AspLEI GCGC 1 cut(s) 202
AspS9I GGNCC 1 cut(s) 351
AsuHPI GGTGA 2 cut(s) 64, 73
AsuII TTCGAA 1 cut(s) 423
AvaII GGWCC 1 cut(s) 351
BaeGI GKGCMC 1 cut(s) 114
BaeI ACNNNNGTAYC 2 cut(s) 258, 291
BbvI GCAGC 1 cut(s) 50
BccI CCATC 4 cut(s) 52, 101, 181, 218
BceAI ACGGC 1 cut(s) 213
BciT130I CCWGG 2 cut(s) 355, 407
BclI TGATCA 1 cut(s) 136
BcoDI GTCTC 2 cut(s) 86, 234
BfmI CTRYAG 1 cut(s) 144
BfoI RGCGCY 1 cut(s) 203
BfuAI ACCTGC 1 cut(s) 334
BisI GCNGC 1 cut(s) 39
BlsI GCNGC 1 cut(s) 40
Bme1390I CCNGG 2 cut(s) 355, 407
Bme18I GGWCC 1 cut(s) 351
BmgT120I GGNCC 1 cut(s) 351
BmrFI CCNGG 2 cut(s) 355, 407
BmsI GCATC 1 cut(s) 315
Bpu14I TTCGAA 1 cut(s) 423
BsaJI CCNNGG 2 cut(s) 214, 406
BsaWI WCCGGW 1 cut(s) 304
BsaXI ACNNNNNCTCC 2 cut(s) 291, 321
Bsc4I CCNNNNNNNGG 3 cut(s) 216, 295, 412
Bse118I RCCGGY 1 cut(s) 304
BseBI CCWGG 2 cut(s) 355, 407
BseDI CCNNGG 2 cut(s) 214, 406
BseGI GGATG 1 cut(s) 210
BseLI CCNNNNNNNGG 3 cut(s) 216, 295, 412
BseRI GAGGAG 1 cut(s) 388
BseSI GKGCMC 1 cut(s) 114
BseXI GCAGC 1 cut(s) 50
BshFI GGCC 2 cut(s) 405, 435
BshTI ACCGGT 1 cut(s) 304
BsiSI CCGG 1 cut(s) 305
BslFI GGGAC 2 cut(s) 200, 423
BslI CCNNNNNNNGG 3 cut(s) 216, 295, 412
BsmAI GTCTC 2 cut(s) 86, 234
BsmBI CGTCTC 1 cut(s) 86
BsmFI GGGAC 2 cut(s) 200, 423
BsnI GGCC 2 cut(s) 405, 435
Bsp119I TTCGAA 1 cut(s) 423
Bsp1286I GDGCHC 1 cut(s) 114
Bsp143I GATC 1 cut(s) 136
BspACI CCGC 1 cut(s) 328
BspANI GGCC 2 cut(s) 405, 435
BspHI TCATGA 1 cut(s) 313
BspMI ACCTGC 1 cut(s) 334
BspT104I TTCGAA 1 cut(s) 423
BsrFI RCCGGY 1 cut(s) 304
BssAI RCCGGY 1 cut(s) 304
BssECI CCNNGG 2 cut(s) 214, 406
BssMI GATC 1 cut(s) 136
Bst2UI CCWGG 2 cut(s) 355, 407
BstBI TTCGAA 1 cut(s) 423
BstDSI CCRYGG 1 cut(s) 214
BstF5I GGATG 1 cut(s) 210
BstH2I RGCGCY 1 cut(s) 203
BstHHI GCGC 1 cut(s) 202
BstKTI GATC 1 cut(s) 139
BstMAI GTCTC 2 cut(s) 86, 234
BstMBI GATC 1 cut(s) 136
BstMWI GCNNNNNNNGC 1 cut(s) 340
BstNI CCWGG 2 cut(s) 355, 407
BstSCI CCNGG 2 cut(s) 353, 405
BstSFI CTRYAG 1 cut(s) 144
BstSLI GKGCMC 1 cut(s) 114
BstV1I GCAGC 1 cut(s) 50
BsuRI GGCC 2 cut(s) 405, 435
BtgI CCRYGG 1 cut(s) 214
BtsCI GGATG 1 cut(s) 210
BtsIMutI CAGTG 1 cut(s) 165
BveI ACCTGC 1 cut(s) 334
CciI TCATGA 1 cut(s) 313
CfoI GCGC 1 cut(s) 202
Cfr10I RCCGGY 1 cut(s) 304
Cfr13I GGNCC 1 cut(s) 351
Csp6I GTAC 1 cut(s) 275
CspAI ACCGGT 1 cut(s) 304
CviAII CATG 2 cut(s) 134, 314
CviJI RGCY 5 cut(s) 41, 334, 358, 405, 435
CviKI_1 RGCY 5 cut(s) 41, 334, 358, 405, 435
CviQI GTAC 1 cut(s) 275
DpnI GATC 1 cut(s) 138
DpnII GATC 1 cut(s) 136
Eco47I GGWCC 1 cut(s) 351
EcoRII CCWGG 2 cut(s) 353, 405
Esp3I CGTCTC 1 cut(s) 86
FaeI CATG 2 cut(s) 137, 317
FaiI YATR 8 cut(s) 78, 119, 135, 141, 146, 182, 246, 315
FaqI GGGAC 2 cut(s) 200, 423
FatI CATG 2 cut(s) 133, 313
FbaI TGATCA 1 cut(s) 136
Fnu4HI GCNGC 1 cut(s) 39
FokI GGATG 1 cut(s) 197
Fsp4HI GCNGC 1 cut(s) 39
GlaI GCGC 1 cut(s) 201
GluI GCNGC 1 cut(s) 39
HaeII RGCGCY 1 cut(s) 203
HaeIII GGCC 2 cut(s) 405, 435
HapII CCGG 1 cut(s) 305
HhaI GCGC 1 cut(s) 202
Hin1II CATG 2 cut(s) 137, 317
Hin6I GCGC 1 cut(s) 200
HinP1I GCGC 1 cut(s) 200
HinfI GANTC 2 cut(s) 188, 281
HpaII CCGG 1 cut(s) 305
HphI GGTGA 2 cut(s) 64, 73
Hpy188I TCNGA 2 cut(s) 85, 431
Hpy188III TCNNGA 1 cut(s) 314
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 2 cut(s) 58, 289
HpyCH4IV ACGT 1 cut(s) 101
HpyCH4V TGCA 1 cut(s) 38
HpyF10VI GCNNNNNNNGC 1 cut(s) 340
HpySE526I ACGT 1 cut(s) 101
Hsp92II CATG 2 cut(s) 137, 317
HspAI GCGC 1 cut(s) 200
Ksp22I TGATCA 1 cut(s) 136
Kzo9I GATC 1 cut(s) 136
LpnPI CCDG 9 cut(s) 188, 318, 329, 340, 348, 367, 381, 392, 419
Lsp1109I GCAGC 1 cut(s) 50
LweI GCATC 1 cut(s) 315
MaeII ACGT 1 cut(s) 101
MaeIII GTNAC 1 cut(s) 52
MalI GATC 1 cut(s) 138
MboI GATC 1 cut(s) 136
MfeI CAATTG 1 cut(s) 270
MhlI GDGCHC 1 cut(s) 114
MluCI AATT 2 cut(s) 32, 270
MlyI GAGTC 2 cut(s) 182, 275
MmeI TCCRAC 2 cut(s) 102, 409
MnlI CCTC 6 cut(s) 15, 43, 310, 360, 366, 425
MroXI GAANNNNTTC 1 cut(s) 306
MspA1I CMGCKG 1 cut(s) 41
MspI CCGG 1 cut(s) 305
MspR9I CCNGG 2 cut(s) 355, 407
MunI CAATTG 1 cut(s) 270
MvaI CCWGG 2 cut(s) 355, 407
MwoI GCNNNNNNNGC 1 cut(s) 340
NdeII GATC 1 cut(s) 136
NlaIII CATG 2 cut(s) 137, 317
NmuCI GTSAC 1 cut(s) 52
NspV TTCGAA 1 cut(s) 423
PagI TCATGA 1 cut(s) 313
PaqCI CACCTGC 1 cut(s) 334
PdmI GAANNNNTTC 1 cut(s) 306
PinAI ACCGGT 1 cut(s) 304
PkrI GCNGC 1 cut(s) 40
PleI GAGTC 2 cut(s) 182, 275
PpsI GAGTC 2 cut(s) 182, 275
Psp1406I AACGTT 1 cut(s) 101
Psp6I CCWGG 2 cut(s) 353, 405
PspGI CCWGG 2 cut(s) 353, 405
PspPI GGNCC 1 cut(s) 351
PvuII CAGCTG 1 cut(s) 41
RsaI GTAC 1 cut(s) 276
RsaNI GTAC 1 cut(s) 275
SatI GCNGC 1 cut(s) 39
Sau3AI GATC 1 cut(s) 136
Sau96I GGNCC 1 cut(s) 351
SchI GAGTC 2 cut(s) 182, 275
ScrFI CCNGG 2 cut(s) 355, 407
SduI GDGCHC 1 cut(s) 114
SetI ASST 8 cut(s) 26, 43, 54, 69, 104, 348, 371, 400
SfaNI GCATC 1 cut(s) 315
SfcI CTRYAG 1 cut(s) 144
SfuI TTCGAA 1 cut(s) 423
SinI GGWCC 1 cut(s) 351
Sse9I AATT 2 cut(s) 32, 270
SsiI CCGC 1 cut(s) 328
StyD4I CCNGG 2 cut(s) 353, 405
TaiI ACGT 1 cut(s) 104
TaqI TCGA 2 cut(s) 105, 423
TasI AATT 2 cut(s) 32, 270
TscAI CASTG 1 cut(s) 165
TseFI GTSAC 1 cut(s) 52
TseI GCWGC 1 cut(s) 38
Tsp45I GTSAC 1 cut(s) 52
TspGWI ACGGA 2 cut(s) 7, 203
TspRI CASTG 1 cut(s) 165
VpaK11BI GGWCC 1 cut(s) 351
XapI RAATTY 1 cut(s) 32
XmnI GAANNNNTTC 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.