Rroxscaffold_4G00286490

40S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0020035
Physical Location & Seq
Reverse (-)
7478346 .. 7478672
327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00286490.1

Sequence Viewer

Length: 327 bp
ATGGCTTCGCCTGTTTGCAACGGCGTGGTTTTGCTGTGGCCAAAAACTGGCCTTTCTCTTCAGCGGCGCGAAATCTCACCAGTGAAGCACGACCTGGTGGTGAAAGTGATGGGCCGGACTGGATCCATAGGTCAGGTGACCCACGGACTTCTCAACTTTCTTGATGAGCAGAACCGGTTCGTCATGAGGAACAGATGCGGAAGCCAGAGAAGCAGGTGTGGTCCTGGCTGTGGAGGAGTGTGGTGGGGATTGGGTCAGGTTTCGGCCTGGGACGGGTCGGGTTCGAAGTCGGAGGCCGATTTGGGTGGAGTGAATTTCGAAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S28e

Protein Analysis

108

Amino Acids

11.59

Weight (kDa)

9.2

Isoelectric Point (pI)

37.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S28e PF01200 29 - 69 8.2e-06 Ribosomal protein S28e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020035)

Species Orthologous Gene IDs
rosa_laevigata RLG00000027030
rosa_multiflora Rmu_sc0002558.1_g000002
rosa_roxburghii Rroxscaffold_4G00286490 Rroxscaffold_4G00286500
rosa_rugosa Rorug01G0357400
rosa_samantha Rh1CG343800 Rh1DG361100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 204
Acc36I ACCTGC 1 cut(s) 204
AccB7I CCANNNNNTGG 1 cut(s) 47
AccII CGCG 1 cut(s) 69
AciI CCGC 2 cut(s) 64, 198
AclWI GGATC 2 cut(s) 117, 130
AcoI YGGCCR 1 cut(s) 38
AcsI RAATTY 1 cut(s) 313
AcuI CTGAAG 1 cut(s) 44
AfiI CCNNNNNNNGG 3 cut(s) 47, 230, 273
AgeI ACCGGT 1 cut(s) 174
AjnI CCWGG 3 cut(s) 93, 223, 266
AlwI GGATC 2 cut(s) 117, 130
AoxI GGCC 5 cut(s) 38, 49, 112, 264, 294
ApoI RAATTY 1 cut(s) 313
AsiGI ACCGGT 1 cut(s) 174
Asp700I GAANNNNTTC 1 cut(s) 176
AspLEI GCGC 1 cut(s) 69
AspS9I GGNCC 2 cut(s) 112, 221
AsuHPI GGTGA 3 cut(s) 69, 112, 148
AsuII TTCGAA 2 cut(s) 284, 318
AvaII GGWCC 1 cut(s) 221
BalI TGGCCA 1 cut(s) 40
BamHI GGATCC 1 cut(s) 122
BccI CCATC 1 cut(s) 103
BceAI ACGGC 1 cut(s) 37
BciT130I CCWGG 3 cut(s) 95, 225, 268
BfuAI ACCTGC 1 cut(s) 204
BisI GCNGC 1 cut(s) 65
BlsI GCNGC 1 cut(s) 66
Bme1390I CCNGG 3 cut(s) 95, 225, 268
Bme18I GGWCC 1 cut(s) 221
BmgT120I GGNCC 2 cut(s) 112, 221
BmiI GGNNCC 1 cut(s) 124
BmrFI CCNGG 3 cut(s) 95, 225, 268
BmsI GCATC 1 cut(s) 185
Bpu14I TTCGAA 2 cut(s) 284, 318
BsaJI CCNNGG 2 cut(s) 142, 267
BsaWI WCCGGW 1 cut(s) 174
Bsc4I CCNNNNNNNGG 3 cut(s) 47, 230, 273
Bse118I RCCGGY 1 cut(s) 174
Bse1I ACTGG 3 cut(s) 52, 80, 124
BseBI CCWGG 3 cut(s) 95, 225, 268
BseDI CCNNGG 2 cut(s) 142, 267
BseLI CCNNNNNNNGG 3 cut(s) 47, 230, 273
BseNI ACTGG 3 cut(s) 52, 80, 124
BseRI GAGGAG 1 cut(s) 249
Bsh1236I CGCG 1 cut(s) 69
BshFI GGCC 5 cut(s) 40, 51, 114, 266, 296
BshTI ACCGGT 1 cut(s) 174
BsiSI CCGG 2 cut(s) 115, 175
BslFI GGGAC 1 cut(s) 284
BslI CCNNNNNNNGG 3 cut(s) 47, 230, 273
BsmFI GGGAC 1 cut(s) 284
BsnI GGCC 5 cut(s) 40, 51, 114, 266, 296
Bsp119I TTCGAA 2 cut(s) 284, 318
Bsp143I GATC 1 cut(s) 122
BspACI CCGC 2 cut(s) 64, 198
BspANI GGCC 5 cut(s) 40, 51, 114, 266, 296
BspFNI CGCG 1 cut(s) 69
BspHI TCATGA 1 cut(s) 183
BspLI GGNNCC 1 cut(s) 124
BspMI ACCTGC 1 cut(s) 204
BspPI GGATC 2 cut(s) 117, 130
BspT104I TTCGAA 2 cut(s) 284, 318
BsrFI RCCGGY 1 cut(s) 174
BsrI ACTGG 3 cut(s) 52, 80, 124
BssAI RCCGGY 1 cut(s) 174
BssECI CCNNGG 2 cut(s) 142, 267
BssMI GATC 1 cut(s) 122
Bst2UI CCWGG 3 cut(s) 95, 225, 268
Bst6I CTCTTC 1 cut(s) 63
BstBI TTCGAA 2 cut(s) 284, 318
BstDSI CCRYGG 1 cut(s) 142
BstEII GGTNACC 1 cut(s) 136
BstFNI CGCG 1 cut(s) 69
BstHHI GCGC 1 cut(s) 69
BstKTI GATC 1 cut(s) 125
BstMBI GATC 1 cut(s) 122
BstMWI GCNNNNNNNGC 1 cut(s) 210
BstNI CCWGG 3 cut(s) 95, 225, 268
BstPI GGTNACC 1 cut(s) 136
BstSCI CCNGG 3 cut(s) 93, 223, 266
BstUI CGCG 1 cut(s) 69
BstX2I RGATCY 1 cut(s) 122
BstYI RGATCY 1 cut(s) 122
BsuRI GGCC 5 cut(s) 40, 51, 114, 266, 296
BtgI CCRYGG 1 cut(s) 142
BtsIMutI CAGTG 1 cut(s) 87
BveI ACCTGC 1 cut(s) 204
CciI TCATGA 1 cut(s) 183
CfoI GCGC 1 cut(s) 69
Cfr10I RCCGGY 1 cut(s) 174
Cfr13I GGNCC 2 cut(s) 112, 221
CsiI ACCWGGT 1 cut(s) 93
CspAI ACCGGT 1 cut(s) 174
CviAII CATG 1 cut(s) 184
CviJI RGCY 8 cut(s) 5, 40, 51, 114, 204, 228, 266, 296
CviKI_1 RGCY 8 cut(s) 5, 40, 51, 114, 204, 228, 266, 296
DpnI GATC 1 cut(s) 124
DpnII GATC 1 cut(s) 122
EaeI YGGCCR 1 cut(s) 38
Eam1104I CTCTTC 1 cut(s) 63
EarI CTCTTC 1 cut(s) 63
Eco47I GGWCC 1 cut(s) 221
Eco57I CTGAAG 1 cut(s) 44
Eco91I GGTNACC 1 cut(s) 136
EcoO65I GGTNACC 1 cut(s) 136
EcoRII CCWGG 3 cut(s) 93, 223, 266
FaeI CATG 1 cut(s) 187
FaiI YATR 2 cut(s) 128, 185
FaqI GGGAC 1 cut(s) 284
FatI CATG 1 cut(s) 183
Fnu4HI GCNGC 1 cut(s) 65
Fsp4HI GCNGC 1 cut(s) 65
GlaI GCGC 1 cut(s) 68
GluI GCNGC 1 cut(s) 65
HaeIII GGCC 5 cut(s) 40, 51, 114, 266, 296
HapII CCGG 2 cut(s) 115, 175
HhaI GCGC 1 cut(s) 69
Hin1II CATG 1 cut(s) 187
Hin6I GCGC 1 cut(s) 67
HinP1I GCGC 1 cut(s) 67
HpaII CCGG 2 cut(s) 115, 175
HphI GGTGA 3 cut(s) 69, 112, 148
Hpy188I TCNGA 1 cut(s) 292
Hpy188III TCNNGA 2 cut(s) 161, 184
HpyAV CCTTC 1 cut(s) 314
HpyCH4V TGCA 1 cut(s) 18
HpyF10VI GCNNNNNNNGC 1 cut(s) 210
Hsp92II CATG 1 cut(s) 187
HspAI GCGC 1 cut(s) 67
Kzo9I GATC 1 cut(s) 122
LweI GCATC 1 cut(s) 185
MabI ACCWGGT 1 cut(s) 93
MaeIII GTNAC 1 cut(s) 136
MalI GATC 1 cut(s) 124
MboI GATC 1 cut(s) 122
MboII GAAGA 1 cut(s) 50
MflI RGATCY 1 cut(s) 122
MlsI TGGCCA 1 cut(s) 40
MluCI AATT 1 cut(s) 313
MluNI TGGCCA 1 cut(s) 40
MmeI TCCRAC 1 cut(s) 270
MnlI CCTC 3 cut(s) 180, 227, 286
Mox20I TGGCCA 1 cut(s) 40
MroXI GAANNNNTTC 1 cut(s) 176
MscI TGGCCA 1 cut(s) 40
Msp20I TGGCCA 1 cut(s) 40
MspA1I CMGCKG 1 cut(s) 64
MspI CCGG 2 cut(s) 115, 175
MspR9I CCNGG 3 cut(s) 95, 225, 268
MvaI CCWGG 3 cut(s) 95, 225, 268
MvnI CGCG 1 cut(s) 69
MwoI GCNNNNNNNGC 1 cut(s) 210
NdeII GATC 1 cut(s) 122
NlaIII CATG 1 cut(s) 187
NlaIV GGNNCC 1 cut(s) 124
NmuCI GTSAC 1 cut(s) 136
NspV TTCGAA 2 cut(s) 284, 318
PagI TCATGA 1 cut(s) 183
PaqCI CACCTGC 1 cut(s) 204
PdmI GAANNNNTTC 1 cut(s) 176
PflMI CCANNNNNTGG 1 cut(s) 47
PinAI ACCGGT 1 cut(s) 174
PkrI GCNGC 1 cut(s) 66
Psp6I CCWGG 3 cut(s) 93, 223, 266
PspEI GGTNACC 1 cut(s) 136
PspGI CCWGG 3 cut(s) 93, 223, 266
PspN4I GGNNCC 1 cut(s) 124
PspPI GGNCC 2 cut(s) 112, 221
PsuI RGATCY 1 cut(s) 122
SatI GCNGC 1 cut(s) 65
Sau3AI GATC 1 cut(s) 122
Sau96I GGNCC 2 cut(s) 112, 221
ScrFI CCNGG 3 cut(s) 95, 225, 268
SetI ASST 5 cut(s) 96, 133, 138, 218, 261
SexAI ACCWGGT 1 cut(s) 93
SfaNI GCATC 1 cut(s) 185
SfuI TTCGAA 2 cut(s) 284, 318
SinI GGWCC 1 cut(s) 221
Sse9I AATT 1 cut(s) 313
SsiI CCGC 2 cut(s) 64, 198
StyD4I CCNGG 3 cut(s) 93, 223, 266
TaqI TCGA 2 cut(s) 284, 318
TasI AATT 1 cut(s) 313
TauI GCSGC 1 cut(s) 67
TscAI CASTG 1 cut(s) 87
TseFI GTSAC 1 cut(s) 136
Tsp45I GTSAC 1 cut(s) 136
TspGWI ACGGA 1 cut(s) 159
TspRI CASTG 1 cut(s) 87
Van91I CCANNNNNTGG 1 cut(s) 47
VpaK11BI GGWCC 1 cut(s) 221
XapI RAATTY 1 cut(s) 313
XmnI GAANNNNTTC 1 cut(s) 176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.