RLG00000027445

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0018874
Physical Location & Seq
Reverse (-)
11727315 .. 11728107
793 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027445

Sequence Viewer

Length: 624 bp
ATGTCTGAGTTCCAACTTCAAGCGTCGGTCCTCAAGGAAATTCTGGTGTTCATGGAGACGATGAAGGTGAATGGCGAGGAATTCATCGAGACAATGAATGGCGTCAACTATTGCTCGAGAACCGAGCTCGACGGTTGCTGGCCAACCGAGCTCTTCGATTGCTCGCCAACCGAGCTCTGCCTCCAAGCCTTGGATCCCAGCTATTGTCTCTGGTGGCCTTGCGGCTCAGACCTGACGCCTTCAAGCACTACAGCTGCGATCGGCCCATCTCTTTGGGAATTGTCCTCCCAAGCATGGCCAGGATCCTTAACTTTGCCTCAAACGACGGCGACGTCGTCACCATCAAGGCTGACGATGGCGATAATAGTGGGTCTCATAAATACCAAGCAACTGTTAGCTAGGATGCTTTCGGCTAAGTTTGTCAGGATAATCAAAAGCTTGATTGACGGTCATGGTCGTGATGATATTTTTACGATCTCTGTGACCAGTGATGGGGTGAAGTTCCGTACAAGCGGCCGCGAGGGGCCTGAAGAAGAAGAAGAAGAAGCAACAGTTATAGAAATGAATGAGCCGGTTTTCGATCGAATATCAGTTGAGCTGCTTGAAGCCGTTTACAGAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

208

Amino Acids

22.97

Weight (kDa)

4.4

Isoelectric Point (pI)

55.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018874)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g20850
rosa_chinensis RchiOBHm_Chr1g0365091
rosa_laevigata RLG00000027445
rosa_multiflora Rmu_sc0008025.1_g000005 Rmu_ssc0000112.1_g000005
rosa_rugosa Rorug01G0319500
rosa_samantha Rh1BG289900 Rh1CG306600 Rh1DG321500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 331
AatII GACGTC 1 cut(s) 335
AccB7I CCANNNNNTGG 1 cut(s) 190
AccII CGCG 1 cut(s) 519
AciI CCGC 3 cut(s) 222, 513, 517
AclWI GGATC 4 cut(s) 188, 201, 297, 310
AcoI YGGCCR 3 cut(s) 140, 296, 514
AcsI RAATTY 2 cut(s) 39, 80
AcuI CTGAAG 1 cut(s) 549
AcyI GRCGYC 3 cut(s) 102, 236, 332
AfaI GTAC 1 cut(s) 508
AfiI CCNNNNNNNGG 3 cut(s) 190, 294, 492
AgsI TTSAA 3 cut(s) 20, 243, 605
AjnI CCWGG 1 cut(s) 298
AluBI AGCT 9 cut(s) 127, 151, 175, 201, 254, 398, 438, 598, 621
AluI AGCT 9 cut(s) 127, 151, 175, 201, 254, 398, 438, 598, 621
Alw21I GWGCWC 3 cut(s) 129, 153, 177
Alw26I GTCTC 4 cut(s) 50, 83, 212, 377
AlwI GGATC 4 cut(s) 188, 201, 297, 310
Ama87I CYCGRG 1 cut(s) 115
AoxI GGCC 6 cut(s) 140, 215, 262, 296, 514, 524
ApeKI GCWGC 2 cut(s) 254, 598
ApoI RAATTY 2 cut(s) 39, 80
AspS9I GGNCC 3 cut(s) 28, 263, 524
AsuHPI GGTGA 3 cut(s) 79, 330, 508
AvaI CYCGRG 1 cut(s) 115
AvaII GGWCC 1 cut(s) 28
BalI TGGCCA 2 cut(s) 142, 298
BamHI GGATCC 2 cut(s) 193, 302
BanII GRGCYC 3 cut(s) 129, 153, 177
Bbv12I GWGCWC 3 cut(s) 129, 153, 177
BbvI GCAGC 2 cut(s) 241, 585
BccI CCATC 4 cut(s) 274, 349, 349, 485
BceAI ACGGC 2 cut(s) 342, 593
BciT130I CCWGG 1 cut(s) 300
BcoDI GTCTC 4 cut(s) 50, 83, 212, 377
BfaI CTAG 2 cut(s) 399, 622
BfmI CTRYAG 1 cut(s) 249
BisI GCNGC 5 cut(s) 223, 255, 514, 517, 599
BlsI GCNGC 5 cut(s) 224, 256, 515, 518, 600
Bme1390I CCNGG 1 cut(s) 300
Bme18I GGWCC 1 cut(s) 28
BmeT110I CYCGRG 1 cut(s) 115
BmgT120I GGNCC 3 cut(s) 28, 263, 524
BmiI GGNNCC 3 cut(s) 195, 304, 525
BmrFI CCNGG 1 cut(s) 300
BmsI GCATC 1 cut(s) 393
BpuEI CTTGAG 1 cut(s) 17
BsaHI GRCGYC 3 cut(s) 102, 236, 332
BsaI GGTCTC 1 cut(s) 377
BsaJI CCNNGG 1 cut(s) 189
Bsc4I CCNNNNNNNGG 3 cut(s) 190, 294, 492
Bse118I RCCGGY 1 cut(s) 571
Bse1I ACTGG 1 cut(s) 486
BseBI CCWGG 1 cut(s) 300
BseDI CCNNGG 1 cut(s) 189
BseGI GGATG 1 cut(s) 408
BseLI CCNNNNNNNGG 3 cut(s) 190, 294, 492
BseMII CTCAG 1 cut(s) 240
BseNI ACTGG 1 cut(s) 486
BseX3I CGGCCG 1 cut(s) 514
BseXI GCAGC 2 cut(s) 241, 585
BseYI CCCAGC 1 cut(s) 197
Bsh1236I CGCG 1 cut(s) 519
Bsh1285I CGRYCG 3 cut(s) 261, 517, 583
BshFI GGCC 6 cut(s) 142, 217, 264, 298, 516, 526
BsiEI CGRYCG 3 cut(s) 261, 517, 583
BsiHKAI GWGCWC 3 cut(s) 129, 153, 177
BsiHKCI CYCGRG 1 cut(s) 115
BsiSI CCGG 1 cut(s) 572
BslI CCNNNNNNNGG 3 cut(s) 190, 294, 492
BsmAI GTCTC 4 cut(s) 50, 83, 212, 377
BsmBI CGTCTC 1 cut(s) 50
BsnI GGCC 6 cut(s) 142, 217, 264, 298, 516, 526
Bso31I GGTCTC 1 cut(s) 377
BsoBI CYCGRG 1 cut(s) 115
Bsp1286I GDGCHC 3 cut(s) 129, 153, 177
Bsp143I GATC 5 cut(s) 193, 258, 302, 474, 580
BspACI CCGC 3 cut(s) 222, 513, 517
BspANI GGCC 6 cut(s) 142, 217, 264, 298, 516, 526
BspCNI CTCAG 1 cut(s) 239
BspFNI CGCG 1 cut(s) 519
BspLI GGNNCC 3 cut(s) 195, 304, 525
BspPI GGATC 4 cut(s) 188, 201, 297, 310
BspQI GCTCTTC 1 cut(s) 158
BspTNI GGTCTC 1 cut(s) 377
BsrFI RCCGGY 1 cut(s) 571
BsrI ACTGG 1 cut(s) 486
BssAI RCCGGY 1 cut(s) 571
BssECI CCNNGG 1 cut(s) 189
BssMI GATC 5 cut(s) 193, 258, 302, 474, 580
BssNI GRCGYC 3 cut(s) 102, 236, 332
BssT1I CCWWGG 1 cut(s) 189
Bst2UI CCWGG 1 cut(s) 300
Bst4CI ACNGT 4 cut(s) 134, 393, 449, 553
Bst6I CTCTTC 1 cut(s) 158
BstACI GRCGYC 3 cut(s) 102, 236, 332
BstC8I GCNNGC 2 cut(s) 140, 164
BstDEI CTNAG 3 cut(s) 6, 226, 414
BstF5I GGATG 1 cut(s) 408
BstFNI CGCG 1 cut(s) 519
BstKTI GATC 5 cut(s) 196, 261, 305, 477, 583
BstMAI GTCTC 4 cut(s) 50, 83, 212, 377
BstMBI GATC 5 cut(s) 193, 258, 302, 474, 580
BstMCI CGRYCG 3 cut(s) 261, 517, 583
BstMWI GCNNNNNNNGC 2 cut(s) 148, 172
BstNI CCWGG 1 cut(s) 300
BstSCI CCNGG 1 cut(s) 298
BstSFI CTRYAG 1 cut(s) 249
BstUI CGCG 1 cut(s) 519
BstV1I GCAGC 2 cut(s) 241, 585
BstX2I RGATCY 2 cut(s) 193, 302
BstXI CCANNNNNNTGG 1 cut(s) 273
BstYI RGATCY 2 cut(s) 193, 302
BstZI CGGCCG 1 cut(s) 514
BsuRI GGCC 6 cut(s) 142, 217, 264, 298, 516, 526
BtsCI GGATG 1 cut(s) 408
BtsIMutI CAGTG 1 cut(s) 493
Cac8I GCNNGC 2 cut(s) 140, 164
CciNI GCGGCCGC 1 cut(s) 514
Cfr10I RCCGGY 1 cut(s) 571
Cfr13I GGNCC 3 cut(s) 28, 263, 524
CseI GACGC 3 cut(s) 12, 91, 244
Csp6I GTAC 1 cut(s) 507
CviAII CATG 3 cut(s) 52, 294, 452
CviQI GTAC 1 cut(s) 507
DdeI CTNAG 3 cut(s) 6, 226, 414
DpnI GATC 5 cut(s) 195, 260, 304, 476, 582
DpnII GATC 5 cut(s) 193, 258, 302, 474, 580
DrdI GACNNNNNNGTC 1 cut(s) 331
DseDI GACNNNNNNGTC 1 cut(s) 331
EaeI YGGCCR 3 cut(s) 140, 296, 514
EagI CGGCCG 1 cut(s) 514
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
Ecl136II GAGCTC 3 cut(s) 127, 151, 175
EclXI CGGCCG 1 cut(s) 514
Eco130I CCWWGG 1 cut(s) 189
Eco24I GRGCYC 3 cut(s) 129, 153, 177
Eco31I GGTCTC 1 cut(s) 377
Eco47I GGWCC 1 cut(s) 28
Eco52I CGGCCG 1 cut(s) 514
Eco53kI GAGCTC 3 cut(s) 127, 151, 175
Eco57I CTGAAG 1 cut(s) 549
Eco88I CYCGRG 1 cut(s) 115
EcoICRI GAGCTC 3 cut(s) 127, 151, 175
EcoO109I RGGNCCY 1 cut(s) 524
EcoRI GAATTC 1 cut(s) 80
EcoRII CCWGG 1 cut(s) 298
EcoT14I CCWWGG 1 cut(s) 189
EcoT38I GRGCYC 3 cut(s) 129, 153, 177
ErhI CCWWGG 1 cut(s) 189
Esp3I CGTCTC 1 cut(s) 50
FaeI CATG 3 cut(s) 55, 297, 455
FaiI YATR 5 cut(s) 53, 295, 377, 453, 557
FatI CATG 3 cut(s) 51, 293, 451
Fnu4HI GCNGC 5 cut(s) 223, 255, 514, 517, 599
FokI GGATG 1 cut(s) 415
FriOI GRGCYC 3 cut(s) 129, 153, 177
Fsp4HI GCNGC 5 cut(s) 223, 255, 514, 517, 599
FspBI CTAG 2 cut(s) 399, 622
GluI GCNGC 5 cut(s) 223, 255, 514, 517, 599
GsaI CCCAGC 1 cut(s) 201
HaeIII GGCC 6 cut(s) 142, 217, 264, 298, 516, 526
HapII CCGG 1 cut(s) 572
HgaI GACGC 3 cut(s) 12, 91, 244
Hin1I GRCGYC 3 cut(s) 102, 236, 332
Hin1II CATG 3 cut(s) 55, 297, 455
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HindIII AAGCTT 1 cut(s) 436
HpaII CCGG 1 cut(s) 572
HphI GGTGA 3 cut(s) 79, 330, 508
Hpy166II GTNNAC 2 cut(s) 106, 613
Hpy188I TCNGA 2 cut(s) 7, 229
Hpy188III TCNNGA 4 cut(s) 88, 117, 424, 458
Hpy8I GTNNAC 2 cut(s) 106, 613
Hpy99I CGWCG 5 cut(s) 28, 134, 328, 334, 337
HpyAV CCTTC 2 cut(s) 58, 249
HpyCH4III ACNGT 4 cut(s) 134, 393, 449, 553
HpyCH4IV ACGT 1 cut(s) 332
HpyF10VI GCNNNNNNNGC 2 cut(s) 148, 172
HpyF3I CTNAG 3 cut(s) 6, 226, 414
HpySE526I ACGT 1 cut(s) 332
Hsp92I GRCGYC 3 cut(s) 102, 236, 332
Hsp92II CATG 3 cut(s) 55, 297, 455
Kzo9I GATC 5 cut(s) 193, 258, 302, 474, 580
LguI GCTCTTC 1 cut(s) 158
Lsp1109I GCAGC 2 cut(s) 241, 585
LweI GCATC 1 cut(s) 393
MaeI CTAG 2 cut(s) 399, 622
MaeII ACGT 1 cut(s) 332
MaeIII GTNAC 2 cut(s) 336, 481
MalI GATC 5 cut(s) 195, 260, 304, 476, 582
MboI GATC 5 cut(s) 193, 258, 302, 474, 580
MboII GAAGA 6 cut(s) 145, 542, 545, 548, 551, 554
MflI RGATCY 2 cut(s) 193, 302
MhlI GDGCHC 3 cut(s) 129, 153, 177
MlsI TGGCCA 2 cut(s) 142, 298
MluCI AATT 3 cut(s) 39, 80, 278
MluNI TGGCCA 2 cut(s) 142, 298
MmeI TCCRAC 1 cut(s) 37
MnlI CCTC 6 cut(s) 41, 70, 191, 295, 327, 514
Mox20I TGGCCA 2 cut(s) 142, 298
MscI TGGCCA 2 cut(s) 142, 298
MseI TTAA 1 cut(s) 308
MslI CAYNNNNRTG 1 cut(s) 456
Msp20I TGGCCA 2 cut(s) 142, 298
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 1 cut(s) 572
MspR9I CCNGG 1 cut(s) 300
MvaI CCWGG 1 cut(s) 300
MvnI CGCG 1 cut(s) 519
MwoI GCNNNNNNNGC 2 cut(s) 148, 172
NdeII GATC 5 cut(s) 193, 258, 302, 474, 580
NlaIII CATG 3 cut(s) 55, 297, 455
NlaIV GGNNCC 3 cut(s) 195, 304, 525
NmuCI GTSAC 2 cut(s) 336, 481
NotI GCGGCCGC 1 cut(s) 514
PaeR7I CTCGAG 1 cut(s) 115
PciSI GCTCTTC 1 cut(s) 158
PcsI WCGNNNNNNNCGW 2 cut(s) 329, 332
PflFI GACNNNGTC 1 cut(s) 334
PflMI CCANNNNNTGG 1 cut(s) 190
PkrI GCNGC 5 cut(s) 224, 256, 515, 518, 600
Ple19I CGATCG 2 cut(s) 261, 583
Psp124BI GAGCTC 3 cut(s) 129, 153, 177
Psp6I CCWGG 1 cut(s) 298
PspFI CCCAGC 1 cut(s) 197
PspGI CCWGG 1 cut(s) 298
PspN4I GGNNCC 3 cut(s) 195, 304, 525
PspPI GGNCC 3 cut(s) 28, 263, 524
PsuI RGATCY 2 cut(s) 193, 302
PsyI GACNNNGTC 1 cut(s) 334
PvuI CGATCG 2 cut(s) 261, 583
PvuII CAGCTG 1 cut(s) 254
RsaI GTAC 1 cut(s) 508
RsaNI GTAC 1 cut(s) 507
RseI CAYNNNNRTG 1 cut(s) 456
SacI GAGCTC 3 cut(s) 129, 153, 177
SapI GCTCTTC 1 cut(s) 158
SaqAI TTAA 1 cut(s) 308
SatI GCNGC 5 cut(s) 223, 255, 514, 517, 599
Sau3AI GATC 5 cut(s) 193, 258, 302, 474, 580
Sau96I GGNCC 3 cut(s) 28, 263, 524
ScrFI CCNGG 1 cut(s) 300
SduI GDGCHC 3 cut(s) 129, 153, 177
SfaNI GCATC 1 cut(s) 393
SfcI CTRYAG 1 cut(s) 249
Sfr274I CTCGAG 1 cut(s) 115
SinI GGWCC 1 cut(s) 28
SlaI CTCGAG 1 cut(s) 115
SmiMI CAYNNNNRTG 1 cut(s) 456
SmlI CTYRAG 2 cut(s) 32, 115
SmoI CTYRAG 2 cut(s) 32, 115
Sse9I AATT 3 cut(s) 39, 80, 278
SsiI CCGC 3 cut(s) 222, 513, 517
SspMI CTAG 2 cut(s) 399, 622
SstI GAGCTC 3 cut(s) 129, 153, 177
StyD4I CCNGG 1 cut(s) 298
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 4 cut(s) 134, 393, 449, 553
TaiI ACGT 1 cut(s) 335
TaqI TCGA 6 cut(s) 87, 116, 129, 156, 579, 583
TaqII GACCGA 1 cut(s) 16
TasI AATT 3 cut(s) 39, 80, 278
TauI GCSGC 3 cut(s) 225, 516, 519
Tru1I TTAA 1 cut(s) 308
Tru9I TTAA 1 cut(s) 308
TscAI CASTG 1 cut(s) 493
TseFI GTSAC 2 cut(s) 336, 481
TseI GCWGC 2 cut(s) 254, 598
Tsp45I GTSAC 2 cut(s) 336, 481
TspDTI ATGAA 5 cut(s) 40, 73, 77, 110, 578
TspGWI ACGGA 1 cut(s) 494
TspRI CASTG 1 cut(s) 493
Tth111I GACNNNGTC 1 cut(s) 334
Van91I CCANNNNNTGG 1 cut(s) 190
VpaK11BI GGWCC 1 cut(s) 28
XapI RAATTY 2 cut(s) 39, 80
XhoI CTCGAG 1 cut(s) 115
XspI CTAG 2 cut(s) 399, 622
ZraI GACGTC 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.