RLG00000027533

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0015692
Physical Location & Seq
Forward (+)
12464934 .. 12465608
675 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027533

Sequence Viewer

Length: 675 bp
ATGGCCGACTCCAACAACAAAGACCTCTACGACGCCGACGACACCCTCTCCTTCTGTGACTTCTCCATCTACAACGACGACTACGACAACAAGTACTCCTCTCCTCGGAAATACCCAGAGTCTCCAGACCAGGACATGTTCGAGTTTTTCATCGACCATGACTTCGAGCTCGACTCAACAACAATGGATACCATCGTCTTCTGCGGCAAAACCATGAACAACACCACAACAAAGCAACCTAAACTGTTCACAAGAGACCCGCATAACGGCCTCTTCTTGGTCAAAACTAACTCCTTCAAGCGATCTCAGAGTCTCCGGTCGACCGATCTCGCCACCAAACCCTCGTTCAAGTCTTCACCAGCGACCGCGAGCTGCCGTTACCAGAGTGTCAAGTCTAGGAAGCACAAGATGGTGCTGATCGGATCTTTGTCCAAGCCTCAGCCTAAGATGGTGCTCAGCGATATAAAGAAGAGACAAGGAAGGTTGGCTCCGGCACCGCTGTTTCCGGTCAGCGAAGGCGGAGAACAGGCTGTGGACGGTGGGAGTATGGCCCACAAGTTGAGCTTGCTTCGGCCACTCAGGTGTAGGGCCCATTTCGTGAGCGCGTTGGTGAAGGCCTCCTTTGGTTGCATTCCGTCGCCACGACCCGTCAACGGCCGCGCTGCATTGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

225

Amino Acids

25.02

Weight (kDa)

9.25

Isoelectric Point (pI)

56.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015692)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g20000
malus_domestica MD07G1177600.v1.1
prunus_persica Prupe.2G217000_v2.0.a1
pyrus_communis pycom01g13880 pycom07g17160
rosa_chinensis RchiOBHm_Chr1g0363951
rosa_laevigata RLG00000027533
rosa_multiflora Rmu_co8013202.1_g000001 Rmu_sc0001607.1_g000007 Rmu_ssc0000167.1_g000037
rosa_roxburghii Rroxscaffold_4G00292810
rosa_rugosa Rorug01G0311600
rosa_samantha Rh1AG319400 Rh1BG282500 Rh1CG298900 Rh1DG313900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 493
AccI GTMKAC 1 cut(s) 320
AccII CGCG 3 cut(s) 368, 605, 660
AciI CCGC 6 cut(s) 204, 260, 366, 497, 519, 658
AclWI GGATC 1 cut(s) 430
AcoI YGGCCR 3 cut(s) 3, 572, 655
AcyI GRCGYC 1 cut(s) 33
AfaI GTAC 1 cut(s) 95
AfiI CCNNNNNNNGG 4 cut(s) 105, 266, 277, 653
AflIII ACRYGT 1 cut(s) 135
AgsI TTSAA 2 cut(s) 298, 349
AjnI CCWGG 1 cut(s) 129
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AleI CACNNNNGTG 1 cut(s) 580
AluBI AGCT 3 cut(s) 169, 372, 564
AluI AGCT 3 cut(s) 169, 372, 564
Alw21I GWGCWC 2 cut(s) 171, 456
Alw26I GTCTC 4 cut(s) 126, 249, 317, 466
AlwI GGATC 1 cut(s) 430
AoxI GGCC 7 cut(s) 3, 268, 549, 572, 588, 615, 655
ApaI GGGCCC 1 cut(s) 592
ApeKI GCWGC 2 cut(s) 372, 662
ArsI GACNNNNNNTTYG 2 cut(s) 146, 178
AspLEI GCGC 2 cut(s) 605, 662
AspS9I GGNCC 3 cut(s) 550, 588, 589
AsuHPI GGTGA 2 cut(s) 348, 622
BaeGI GKGCMC 1 cut(s) 592
BaeI ACNNNNGTAYC 2 cut(s) 180, 213
BanI GGYRCC 1 cut(s) 493
BanII GRGCYC 2 cut(s) 171, 592
BbsI GAAGAC 2 cut(s) 190, 345
Bbv12I GWGCWC 2 cut(s) 171, 456
BbvCI CCTCAGC 1 cut(s) 438
BbvI GCAGC 2 cut(s) 359, 649
BccI CCATC 4 cut(s) 74, 200, 403, 442
BceAI ACGGC 3 cut(s) 283, 360, 670
BciT130I CCWGG 1 cut(s) 131
BciVI GTATCC 1 cut(s) 181
BcoDI GTCTC 4 cut(s) 126, 249, 317, 466
BfaI CTAG 1 cut(s) 396
BfuI GTATCC 1 cut(s) 181
BisI GCNGC 4 cut(s) 205, 373, 658, 663
BlpI GCTNAGC 1 cut(s) 455
BlsI GCNGC 4 cut(s) 206, 374, 659, 664
BmcAI AGTACT 1 cut(s) 95
Bme1390I CCNGG 1 cut(s) 131
BmgT120I GGNCC 3 cut(s) 550, 588, 589
BmiI GGNNCC 3 cut(s) 489, 495, 590
BmrFI CCNGG 1 cut(s) 131
BpiI GAAGAC 2 cut(s) 190, 345
BplI GAGNNNNNCTC 2 cut(s) 158, 190
BpmI CTGGAG 1 cut(s) 108
Bpu10I CCTNAGC 1 cut(s) 438
Bpu1102I GCTNAGC 1 cut(s) 455
BsaHI GRCGYC 1 cut(s) 33
BsaI GGTCTC 1 cut(s) 249
BsaJI CCNNGG 1 cut(s) 104
BsaWI WCCGGW 2 cut(s) 315, 505
BsaXI ACNNNNNCTCC 4 cut(s) 32, 62, 80, 110
Bsc4I CCNNNNNNNGG 4 cut(s) 105, 266, 277, 653
BseBI CCWGG 1 cut(s) 131
BseDI CCNNGG 1 cut(s) 104
BseLI CCNNNNNNNGG 4 cut(s) 105, 266, 277, 653
BseMII CTCAG 4 cut(s) 320, 452, 469, 592
BseRI GAGGAG 2 cut(s) 88, 93
BseSI GKGCMC 1 cut(s) 592
BseX3I CGGCCG 1 cut(s) 655
BseXI GCAGC 2 cut(s) 359, 649
Bsh1236I CGCG 3 cut(s) 368, 605, 660
Bsh1285I CGRYCG 4 cut(s) 320, 324, 366, 658
BshFI GGCC 7 cut(s) 5, 270, 551, 574, 590, 617, 657
BshNI GGYRCC 1 cut(s) 493
BsiEI CGRYCG 4 cut(s) 320, 324, 366, 658
BsiHKAI GWGCWC 2 cut(s) 171, 456
BsiSI CCGG 3 cut(s) 316, 491, 506
BslI CCNNNNNNNGG 4 cut(s) 105, 266, 277, 653
BsmAI GTCTC 4 cut(s) 126, 249, 317, 466
BsmI GAATGC 1 cut(s) 630
BsnI GGCC 7 cut(s) 5, 270, 551, 574, 590, 617, 657
Bso31I GGTCTC 1 cut(s) 249
Bsp120I GGGCCC 1 cut(s) 588
Bsp1286I GDGCHC 3 cut(s) 171, 456, 592
Bsp143I GATC 4 cut(s) 302, 325, 417, 422
Bsp1720I GCTNAGC 1 cut(s) 455
BspACI CCGC 6 cut(s) 204, 260, 366, 497, 519, 658
BspANI GGCC 7 cut(s) 5, 270, 551, 574, 590, 617, 657
BspCNI CTCAG 4 cut(s) 319, 451, 468, 591
BspFNI CGCG 3 cut(s) 368, 605, 660
BspLI GGNNCC 3 cut(s) 489, 495, 590
BspPI GGATC 1 cut(s) 430
BspT107I GGYRCC 1 cut(s) 493
BspTNI GGTCTC 1 cut(s) 249
BssECI CCNNGG 1 cut(s) 104
BssMI GATC 4 cut(s) 302, 325, 417, 422
BssNI GRCGYC 1 cut(s) 33
Bst2UI CCWGG 1 cut(s) 131
Bst4CI ACNGT 2 cut(s) 246, 539
Bst6I CTCTTC 2 cut(s) 278, 464
BstACI GRCGYC 1 cut(s) 33
BstC8I GCNNGC 2 cut(s) 370, 566
BstDEI CTNAG 6 cut(s) 306, 438, 444, 455, 578, 672
BstFNI CGCG 3 cut(s) 368, 605, 660
BstHHI GCGC 2 cut(s) 605, 662
BstKTI GATC 4 cut(s) 305, 328, 420, 425
BstMAI GTCTC 4 cut(s) 126, 249, 317, 466
BstMBI GATC 4 cut(s) 302, 325, 417, 422
BstMCI CGRYCG 4 cut(s) 320, 324, 366, 658
BstNI CCWGG 1 cut(s) 131
BstNSI RCATGY 1 cut(s) 139
BstSCI CCNGG 1 cut(s) 129
BstSLI GKGCMC 1 cut(s) 592
BstUI CGCG 3 cut(s) 368, 605, 660
BstV1I GCAGC 2 cut(s) 359, 649
BstV2I GAAGAC 2 cut(s) 190, 345
BstX2I RGATCY 1 cut(s) 422
BstYI RGATCY 1 cut(s) 422
BstZI CGGCCG 1 cut(s) 655
BsuI GTATCC 1 cut(s) 181
BsuRI GGCC 7 cut(s) 5, 270, 551, 574, 590, 617, 657
Cac8I GCNNGC 2 cut(s) 370, 566
CfoI GCGC 2 cut(s) 605, 662
Cfr13I GGNCC 3 cut(s) 550, 588, 589
CseI GACGC 1 cut(s) 41
Csp6I GTAC 1 cut(s) 94
CviAII CATG 3 cut(s) 136, 158, 214
CviQI GTAC 1 cut(s) 94
DdeI CTNAG 6 cut(s) 306, 438, 444, 455, 578, 672
DpnI GATC 4 cut(s) 304, 327, 419, 424
DpnII GATC 4 cut(s) 302, 325, 417, 422
EaeI YGGCCR 3 cut(s) 3, 572, 655
EagI CGGCCG 1 cut(s) 655
Eam1104I CTCTTC 2 cut(s) 278, 464
EarI CTCTTC 2 cut(s) 278, 464
EciI GGCGGA 1 cut(s) 534
Ecl136II GAGCTC 1 cut(s) 169
EclXI CGGCCG 1 cut(s) 655
Eco147I AGGCCT 1 cut(s) 617
Eco24I GRGCYC 2 cut(s) 171, 592
Eco31I GGTCTC 1 cut(s) 249
Eco52I CGGCCG 1 cut(s) 655
Eco53kI GAGCTC 1 cut(s) 169
EcoICRI GAGCTC 1 cut(s) 169
EcoO109I RGGNCCY 1 cut(s) 588
EcoRII CCWGG 1 cut(s) 129
EcoT38I GRGCYC 2 cut(s) 171, 592
FaeI CATG 3 cut(s) 139, 161, 217
FaiI YATR 6 cut(s) 137, 159, 215, 264, 464, 548
FalI AAGNNNNNCTT 4 cut(s) 548, 580, 605, 637
FatI CATG 3 cut(s) 135, 157, 213
FauI CCCGC 1 cut(s) 267
FblI GTMKAC 1 cut(s) 320
Fnu4HI GCNGC 4 cut(s) 205, 373, 658, 663
FriOI GRGCYC 2 cut(s) 171, 592
Fsp4HI GCNGC 4 cut(s) 205, 373, 658, 663
FspBI CTAG 1 cut(s) 396
GlaI GCGC 2 cut(s) 604, 661
GluI GCNGC 4 cut(s) 205, 373, 658, 663
GsuI CTGGAG 1 cut(s) 108
HaeIII GGCC 7 cut(s) 5, 270, 551, 574, 590, 617, 657
HapII CCGG 3 cut(s) 316, 491, 506
HgaI GACGC 1 cut(s) 41
HhaI GCGC 2 cut(s) 605, 662
Hin1I GRCGYC 1 cut(s) 33
Hin1II CATG 3 cut(s) 139, 161, 217
Hin6I GCGC 2 cut(s) 603, 660
HinP1I GCGC 2 cut(s) 603, 660
HincII GTYRAC 2 cut(s) 321, 652
HindII GTYRAC 2 cut(s) 321, 652
HinfI GANTC 4 cut(s) 8, 119, 173, 310
HpaII CCGG 3 cut(s) 316, 491, 506
HphI GGTGA 2 cut(s) 348, 622
Hpy166II GTNNAC 4 cut(s) 249, 321, 535, 652
Hpy188I TCNGA 3 cut(s) 108, 309, 422
Hpy188III TCNNGA 2 cut(s) 125, 598
Hpy8I GTNNAC 4 cut(s) 249, 321, 535, 652
Hpy99I CGWCG 4 cut(s) 35, 41, 80, 640
HpyAV CCTTC 5 cut(s) 61, 304, 474, 509, 607
HpyCH4III ACNGT 2 cut(s) 246, 539
HpyCH4V TGCA 2 cut(s) 630, 665
HpyF3I CTNAG 6 cut(s) 306, 438, 444, 455, 578, 672
Hsp92I GRCGYC 1 cut(s) 33
Hsp92II CATG 3 cut(s) 139, 161, 217
HspAI GCGC 2 cut(s) 603, 660
Kzo9I GATC 4 cut(s) 302, 325, 417, 422
LmnI GCTCC 1 cut(s) 493
Lsp1109I GCAGC 2 cut(s) 359, 649
MaeI CTAG 1 cut(s) 396
MaeIII GTNAC 2 cut(s) 56, 377
MalI GATC 4 cut(s) 304, 327, 419, 424
MboI GATC 4 cut(s) 302, 325, 417, 422
MboII GAAGA 4 cut(s) 190, 265, 345, 481
MflI RGATCY 1 cut(s) 422
MhlI GDGCHC 3 cut(s) 171, 456, 592
MlyI GAGTC 4 cut(s) 2, 128, 167, 319
MmeI TCCRAC 1 cut(s) 36
MnlI CCTC 8 cut(s) 35, 56, 109, 114, 281, 352, 447, 628
MslI CAYNNNNRTG 1 cut(s) 580
MspA1I CMGCKG 1 cut(s) 499
MspI CCGG 3 cut(s) 316, 491, 506
MspR9I CCNGG 1 cut(s) 131
Mva1269I GAATGC 1 cut(s) 630
MvaI CCWGG 1 cut(s) 131
MvnI CGCG 3 cut(s) 368, 605, 660
NdeII GATC 4 cut(s) 302, 325, 417, 422
NlaIII CATG 3 cut(s) 139, 161, 217
NlaIV GGNNCC 3 cut(s) 489, 495, 590
NmuCI GTSAC 1 cut(s) 56
NspI RCATGY 1 cut(s) 139
OliI CACNNNNGTG 1 cut(s) 580
PceI AGGCCT 1 cut(s) 617
PciI ACATGT 1 cut(s) 135
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 81
PctI GAATGC 1 cut(s) 630
PkrI GCNGC 4 cut(s) 206, 374, 659, 664
PleI GAGTC 4 cut(s) 2, 127, 167, 318
PpsI GAGTC 4 cut(s) 2, 127, 167, 318
PscI ACATGT 1 cut(s) 135
Psp124BI GAGCTC 1 cut(s) 171
Psp6I CCWGG 1 cut(s) 129
PspGI CCWGG 1 cut(s) 129
PspN4I GGNNCC 3 cut(s) 489, 495, 590
PspOMI GGGCCC 1 cut(s) 588
PspPI GGNCC 3 cut(s) 550, 588, 589
PsuI RGATCY 1 cut(s) 422
RsaI GTAC 1 cut(s) 95
RsaNI GTAC 1 cut(s) 94
RseI CAYNNNNRTG 1 cut(s) 580
SacI GAGCTC 1 cut(s) 171
SalI GTCGAC 1 cut(s) 319
SatI GCNGC 4 cut(s) 205, 373, 658, 663
Sau3AI GATC 4 cut(s) 302, 325, 417, 422
Sau96I GGNCC 3 cut(s) 550, 588, 589
ScaI AGTACT 1 cut(s) 95
SchI GAGTC 4 cut(s) 2, 128, 167, 319
ScrFI CCNGG 1 cut(s) 131
SduI GDGCHC 3 cut(s) 171, 456, 592
SetI ASST 7 cut(s) 27, 171, 241, 374, 485, 566, 584
SmiMI CAYNNNNRTG 1 cut(s) 580
SseBI AGGCCT 1 cut(s) 617
SsiI CCGC 6 cut(s) 204, 260, 366, 497, 519, 658
SspMI CTAG 1 cut(s) 396
SstI GAGCTC 1 cut(s) 171
StuI AGGCCT 1 cut(s) 617
StyD4I CCNGG 1 cut(s) 129
TaaI ACNGT 2 cut(s) 246, 539
TaqI TCGA 5 cut(s) 141, 153, 165, 171, 320
TaqII GACCGA 1 cut(s) 338
TatI WGTACW 1 cut(s) 93
TauI GCSGC 2 cut(s) 207, 660
TseFI GTSAC 1 cut(s) 56
TseI GCWGC 2 cut(s) 372, 662
Tsp45I GTSAC 1 cut(s) 56
TspDTI ATGAA 2 cut(s) 139, 230
TspGWI ACGGA 1 cut(s) 624
XceI RCATGY 1 cut(s) 139
XmiI GTMKAC 1 cut(s) 320
XspI CTAG 1 cut(s) 396
ZrmI AGTACT 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.