RLG00000028423

Belongs to the peptidase M24B family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0018021
Physical Location & Seq
Reverse (-)
22632091 .. 22634518
2428 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028423

Sequence Viewer

Length: 477 bp
ATGGATTTCCGTGGAAATTCCGCTTATAACCAGCAAATTGATCCAAATATGAAGCCCCTAAAGCGTATCGATGTCCTTCTCCCCCAAATCGATTCAGCCTTATCAGAGAGTGAGTTCGTTGCTGAATGTTATATGAGGAGGTCCTATATGTCGGGTTTCACTGGGAGTGCTGGAACTGCTGTCGTCACAAAGGATAAAGCAGCTCTTTGGACAGATGGGCGCTATTATCTTCAGGCTGAAAAGCAACTGAGCTCTAGTTGGATTCTCATGCAGAAAACAACAACTAATCTTAATGCAAATGTGAATAATCTTCATGGTACACTACTAGATGGAAAAGAAATTGCAGTGAAGCGGCTCTCAAGAAGTTCTGGACAAGGAATGAAAGAGTTCAAAAATGAAGTGATACTGATTGCAAAACTTCAGCACCGCAATCTTGTAAAGCTTCTTCACAAGTTTCATTTGCTCTTACTCTCTTAA

Protein Analysis

159

Amino Acids

17.86

Weight (kDa)

9.61

Isoelectric Point (pI)

48.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Creatinase_N PF01321 46 - 100 1.9e-08 Creatinase/Prolidase N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018021)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g05630 FvH4_4g17470
rosa_chinensis RchiOBHm_Chr1g0378451
rosa_laevigata RLG00000028423
rosa_multiflora Rmu_sc0026514.1_g000001
rosa_roxburghii Rroxscaffold_1G00071590 Rroxscaffold_1G00071830 Rroxscaffold_2G00138070
rosa_rugosa Rorug02G0090500 Rorug04G0294700
rosa_samantha Rh2AG139300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 27
AciI CCGC 3 cut(s) 21, 352, 427
AclWI GGATC 1 cut(s) 35
AcsI RAATTY 1 cut(s) 16
AcuI CTGAAG 2 cut(s) 215, 404
AfaI GTAC 1 cut(s) 319
AgsI TTSAA 1 cut(s) 391
AluBI AGCT 3 cut(s) 203, 252, 442
AluI AGCT 3 cut(s) 203, 252, 442
Alw21I GWGCWC 1 cut(s) 254
AlwI GGATC 1 cut(s) 35
ApeKI GCWGC 1 cut(s) 200
ApoI RAATTY 1 cut(s) 16
Asp700I GAANNNNTTC 1 cut(s) 386
AspLEI GCGC 1 cut(s) 222
AspS9I GGNCC 1 cut(s) 141
AvaII GGWCC 1 cut(s) 141
BanII GRGCYC 1 cut(s) 254
BarI GAAGNNNNNNTAC 2 cut(s) 429, 461
Bbv12I GWGCWC 1 cut(s) 254
BbvI GCAGC 1 cut(s) 212
BccI CCATC 2 cut(s) 209, 323
BfaI CTAG 2 cut(s) 255, 326
BfoI RGCGCY 1 cut(s) 223
BisI GCNGC 2 cut(s) 201, 353
BlsI GCNGC 2 cut(s) 202, 354
Bme18I GGWCC 1 cut(s) 141
BmgT120I GGNCC 1 cut(s) 141
BmrI ACTGGG 1 cut(s) 171
BmuI ACTGGG 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 343
Bsa29I ATCGAT 2 cut(s) 69, 90
BsaJI CCNNGG 1 cut(s) 10
Bse1I ACTGG 1 cut(s) 166
BseCI ATCGAT 2 cut(s) 69, 90
BseDI CCNNGG 1 cut(s) 10
BseMII CTCAG 1 cut(s) 239
BseNI ACTGG 1 cut(s) 166
BseRI GAGGAG 1 cut(s) 151
BseXI GCAGC 1 cut(s) 212
BshVI ATCGAT 2 cut(s) 69, 90
BsiHKAI GWGCWC 1 cut(s) 254
Bsp1286I GDGCHC 1 cut(s) 254
Bsp143I GATC 1 cut(s) 40
BspACI CCGC 3 cut(s) 21, 352, 427
BspCNI CTCAG 1 cut(s) 240
BspDI ATCGAT 2 cut(s) 69, 90
BspPI GGATC 1 cut(s) 35
BsrI ACTGG 1 cut(s) 166
BssECI CCNNGG 1 cut(s) 10
BssMI GATC 1 cut(s) 40
BstDEI CTNAG 1 cut(s) 248
BstDSI CCRYGG 1 cut(s) 10
BstH2I RGCGCY 1 cut(s) 223
BstHHI GCGC 1 cut(s) 222
BstKTI GATC 1 cut(s) 43
BstMBI GATC 1 cut(s) 40
BstMWI GCNNNNNNNGC 2 cut(s) 61, 176
BstV1I GCAGC 1 cut(s) 212
Bsu15I ATCGAT 2 cut(s) 69, 90
BsuTUI ATCGAT 2 cut(s) 69, 90
BtgI CCRYGG 1 cut(s) 10
BtsI GCAGTG 1 cut(s) 351
BtsIMutI CAGTG 2 cut(s) 159, 351
CfoI GCGC 1 cut(s) 222
Cfr13I GGNCC 1 cut(s) 141
ClaI ATCGAT 2 cut(s) 69, 90
Csp6I GTAC 1 cut(s) 318
CviAII CATG 2 cut(s) 268, 314
CviJI RGCY 7 cut(s) 55, 98, 203, 236, 252, 355, 442
CviKI_1 RGCY 7 cut(s) 55, 98, 203, 236, 252, 355, 442
CviQI GTAC 1 cut(s) 318
DdeI CTNAG 1 cut(s) 248
DpnI GATC 1 cut(s) 42
DpnII GATC 1 cut(s) 40
Ecl136II GAGCTC 1 cut(s) 252
Eco24I GRGCYC 1 cut(s) 254
Eco47I GGWCC 1 cut(s) 141
Eco53kI GAGCTC 1 cut(s) 252
Eco57I CTGAAG 2 cut(s) 215, 404
EcoICRI GAGCTC 1 cut(s) 252
EcoO109I RGGNCCY 1 cut(s) 141
EcoT38I GRGCYC 1 cut(s) 254
FaeI CATG 2 cut(s) 271, 317
FaiI YATR 8 cut(s) 27, 50, 132, 134, 147, 149, 269, 315
FalI AAGNNNNNCTT 2 cut(s) 189, 221
FatI CATG 2 cut(s) 267, 313
Fnu4HI GCNGC 2 cut(s) 201, 353
FriOI GRGCYC 1 cut(s) 254
Fsp4HI GCNGC 2 cut(s) 201, 353
FspBI CTAG 2 cut(s) 255, 326
GlaI GCGC 1 cut(s) 221
GluI GCNGC 2 cut(s) 201, 353
HaeII RGCGCY 1 cut(s) 223
HhaI GCGC 1 cut(s) 222
Hin1II CATG 2 cut(s) 271, 317
Hin6I GCGC 1 cut(s) 220
HinP1I GCGC 1 cut(s) 220
HindIII AAGCTT 1 cut(s) 440
HinfI GANTC 2 cut(s) 92, 262
Hpy166II GTNNAC 1 cut(s) 320
Hpy188I TCNGA 1 cut(s) 106
Hpy188III TCNNGA 2 cut(s) 360, 369
Hpy8I GTNNAC 1 cut(s) 320
HpyAV CCTTC 1 cut(s) 86
HpyCH4V TGCA 4 cut(s) 271, 296, 344, 413
HpyF10VI GCNNNNNNNGC 2 cut(s) 61, 176
HpyF3I CTNAG 1 cut(s) 248
Hsp92II CATG 2 cut(s) 271, 317
HspAI GCGC 1 cut(s) 220
Kzo9I GATC 1 cut(s) 40
LpnPI CCDG 5 cut(s) 44, 147, 156, 218, 354
Lsp1109I GCAGC 1 cut(s) 212
MaeI CTAG 2 cut(s) 255, 326
MaeIII GTNAC 1 cut(s) 184
MalI GATC 1 cut(s) 42
MboI GATC 1 cut(s) 40
MboII GAAGA 3 cut(s) 221, 302, 437
MhlI GDGCHC 1 cut(s) 254
MluCI AATT 3 cut(s) 16, 36, 339
MmeI TCCRAC 1 cut(s) 239
MnlI CCTC 2 cut(s) 129, 132
MroXI GAANNNNTTC 1 cut(s) 386
MseI TTAA 2 cut(s) 291, 475
MwoI GCNNNNNNNGC 2 cut(s) 61, 176
NdeII GATC 1 cut(s) 40
NlaIII CATG 2 cut(s) 271, 317
NmuCI GTSAC 1 cut(s) 184
PdmI GAANNNNTTC 1 cut(s) 386
PfeI GAWTC 2 cut(s) 92, 262
PkrI GCNGC 2 cut(s) 202, 354
PpuMI RGGWCCY 1 cut(s) 141
PsiI TTATAA 1 cut(s) 27
Psp124BI GAGCTC 1 cut(s) 254
Psp5II RGGWCCY 1 cut(s) 141
PspPI GGNCC 1 cut(s) 141
PspPPI RGGWCCY 1 cut(s) 141
RsaI GTAC 1 cut(s) 319
RsaNI GTAC 1 cut(s) 318
SacI GAGCTC 1 cut(s) 254
SaqAI TTAA 2 cut(s) 291, 475
SatI GCNGC 2 cut(s) 201, 353
Sau3AI GATC 1 cut(s) 40
Sau96I GGNCC 1 cut(s) 141
SduI GDGCHC 1 cut(s) 254
SetI ASST 4 cut(s) 143, 205, 254, 444
SinI GGWCC 1 cut(s) 141
SmlI CTYRAG 1 cut(s) 358
SmoI CTYRAG 1 cut(s) 358
Sse9I AATT 3 cut(s) 16, 36, 339
SsiI CCGC 3 cut(s) 21, 352, 427
SspMI CTAG 2 cut(s) 255, 326
SstI GAGCTC 1 cut(s) 254
TaqI TCGA 2 cut(s) 69, 90
TasI AATT 3 cut(s) 16, 36, 339
TauI GCSGC 1 cut(s) 355
TfiI GAWTC 2 cut(s) 92, 262
Tru1I TTAA 2 cut(s) 291, 475
Tru9I TTAA 2 cut(s) 291, 475
TscAI CASTG 2 cut(s) 166, 351
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 1 cut(s) 200
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 5 cut(s) 65, 302, 395, 411, 446
TspRI CASTG 2 cut(s) 166, 351
VpaK11BI GGWCC 1 cut(s) 141
XapI RAATTY 1 cut(s) 16
XmnI GAANNNNTTC 1 cut(s) 386
XspI CTAG 2 cut(s) 255, 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.