RLG00000030040

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0017813
Physical Location & Seq
Reverse (-)
53772597 .. 53774125
1529 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030040

Sequence Viewer

Length: 600 bp
ATGAAGTTCGCGGACTTTTTCGGTCGGGCATTATCTGCAGTGACTTCCGCCCAGTTTCCATGGGTCAAGATGTTCAAGGAGCCAATTGTGGTGAAGATTGCCAATGTGACTTTTCGAAATTTCTATGAGAATTATGCTTTTACTTTTATTGCAGTATTTACTGTTGTAGCAATGGTAGTGCAAAGAAAACCTGATGTCTTGATCATTATATTGCCGACATTGAGGGAAACTTCAAAGTATCAAGGACAAGATAATCTTCCAGTAATTCTATGGATGATGGTTCAGGCTAGTGAAGGAGACCTGGCTTTGGTGTTGTATGCATGGGCACACAATGTATTGCCTCTAGTTTGTGTCAAAAGTAGTAATCCACAGTCCTGTGATCTTGTTCTGCAGCTAGTTGAGATAATTCTGTCTATGCCAAAGGCTGGTACCATTTTAGTAAATGGTGCTGTGAGGAAGGGGGAGTATTTGGTGTCACCTCCTGCATTGGAGATACTTATAGGAGTTATTTTTCCTACATCTTCAGCTAGAGTAAAGGCTACTGAAAGATTTGGGGCCGTATATCCGACCCTAAAGGACGTGGCTTTTGTTGGCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

200

Amino Acids

22.02

Weight (kDa)

9.1

Isoelectric Point (pI)

42.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017813)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0017851
rosa_laevigata RLG00000020318 RLG00000030040
rosa_rugosa Rorug02G0253100 Rorug03G0048400
rosa_samantha Rh1BG268600 Rh1DG298700 Rh4AG245000 Rh4BG250200 Rh6BG057500 Rh6DG335800
rosa_wichuraiana Rw2G043450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 428
AccB1I GGYRCC 1 cut(s) 428
AccB7I CCANNNNNTGG 1 cut(s) 425
AccII CGCG 1 cut(s) 11
AciI CCGC 2 cut(s) 11, 48
AcsI RAATTY 1 cut(s) 118
AcuI CTGAAG 1 cut(s) 507
AfaI GTAC 1 cut(s) 430
AfiI CCNNNNNNNGG 2 cut(s) 307, 425
AgsI TTSAA 2 cut(s) 76, 234
AjiI CACGTC 1 cut(s) 580
AjnI CCWGG 1 cut(s) 300
AluBI AGCT 2 cut(s) 394, 527
AluI AGCT 2 cut(s) 394, 527
Alw26I GTCTC 1 cut(s) 291
AoxI GGCC 1 cut(s) 555
ApeKI GCWGC 1 cut(s) 391
ApoI RAATTY 1 cut(s) 118
ArsI GACNNNNNNTTYG 1 cut(s) 569
Asp718I GGTACC 1 cut(s) 428
AspS9I GGNCC 1 cut(s) 555
AsuHPI GGTGA 2 cut(s) 103, 468
AsuII TTCGAA 1 cut(s) 115
BaeGI GKGCMC 1 cut(s) 328
BanI GGYRCC 1 cut(s) 428
BbvI GCAGC 1 cut(s) 403
BccI CCATC 1 cut(s) 271
BceAI ACGGC 1 cut(s) 542
BciT130I CCWGG 1 cut(s) 302
BclI TGATCA 1 cut(s) 201
BcoDI GTCTC 1 cut(s) 291
BfaI CTAG 4 cut(s) 288, 344, 395, 528
BfmI CTRYAG 2 cut(s) 36, 389
BisI GCNGC 1 cut(s) 392
BlsI GCNGC 1 cut(s) 393
Bme1390I CCNGG 1 cut(s) 302
BmgBI CACGTC 1 cut(s) 580
BmgT120I GGNCC 1 cut(s) 555
BmiI GGNNCC 3 cut(s) 81, 430, 556
BmrFI CCNGG 1 cut(s) 302
BmrI ACTGGG 1 cut(s) 46
BmuI ACTGGG 1 cut(s) 46
Bpu14I TTCGAA 1 cut(s) 115
BsaI GGTCTC 1 cut(s) 291
BsaJI CCNNGG 1 cut(s) 59
Bsc4I CCNNNNNNNGG 2 cut(s) 307, 425
Bse1I ACTGG 2 cut(s) 52, 260
Bse3DI GCAATG 1 cut(s) 177
BseBI CCWGG 1 cut(s) 302
BseDI CCNNGG 1 cut(s) 59
BseGI GGATG 1 cut(s) 279
BseLI CCNNNNNNNGG 2 cut(s) 307, 425
BseMI GCAATG 1 cut(s) 177
BseNI ACTGG 2 cut(s) 52, 260
BseSI GKGCMC 1 cut(s) 328
BseXI GCAGC 1 cut(s) 403
Bsh1236I CGCG 1 cut(s) 11
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 1 cut(s) 557
BshNI GGYRCC 1 cut(s) 428
BsiEI CGRYCG 1 cut(s) 25
BslI CCNNNNNNNGG 2 cut(s) 307, 425
BsmAI GTCTC 1 cut(s) 291
BsnI GGCC 1 cut(s) 557
Bso31I GGTCTC 1 cut(s) 291
Bsp119I TTCGAA 1 cut(s) 115
Bsp1286I GDGCHC 1 cut(s) 328
Bsp143I GATC 2 cut(s) 201, 379
Bsp19I CCATGG 1 cut(s) 59
BspACI CCGC 2 cut(s) 11, 48
BspANI GGCC 1 cut(s) 557
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 3 cut(s) 81, 430, 556
BspMAI CTGCAG 2 cut(s) 40, 393
BspT104I TTCGAA 1 cut(s) 115
BspT107I GGYRCC 1 cut(s) 428
BspTNI GGTCTC 1 cut(s) 291
BsrDI GCAATG 1 cut(s) 177
BsrI ACTGG 2 cut(s) 52, 260
BssECI CCNNGG 1 cut(s) 59
BssMI GATC 2 cut(s) 201, 379
BssT1I CCWWGG 1 cut(s) 59
Bst2UI CCWGG 1 cut(s) 302
Bst4CI ACNGT 2 cut(s) 163, 372
BstAPI GCANNNNNTGC 1 cut(s) 35
BstBI TTCGAA 1 cut(s) 115
BstDSI CCRYGG 1 cut(s) 59
BstF5I GGATG 1 cut(s) 279
BstFNI CGCG 1 cut(s) 11
BstKTI GATC 2 cut(s) 204, 382
BstMAI GTCTC 1 cut(s) 291
BstMBI GATC 2 cut(s) 201, 379
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 1 cut(s) 35
BstNI CCWGG 1 cut(s) 302
BstSCI CCNGG 1 cut(s) 300
BstSFI CTRYAG 2 cut(s) 36, 389
BstSLI GKGCMC 1 cut(s) 328
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 1 cut(s) 403
BsuRI GGCC 1 cut(s) 557
BtgI CCRYGG 1 cut(s) 59
BtrI CACGTC 1 cut(s) 580
BtsCI GGATG 1 cut(s) 279
BtsI GCAGTG 1 cut(s) 45
BtsIMutI CAGTG 1 cut(s) 45
Cfr13I GGNCC 1 cut(s) 555
Csp6I GTAC 1 cut(s) 429
CviAII CATG 2 cut(s) 60, 321
CviQI GTAC 1 cut(s) 429
DpnI GATC 2 cut(s) 203, 381
DpnII GATC 2 cut(s) 201, 379
EciI GGCGGA 1 cut(s) 37
Eco130I CCWWGG 1 cut(s) 59
Eco31I GGTCTC 1 cut(s) 291
Eco57I CTGAAG 1 cut(s) 507
EcoRII CCWGG 1 cut(s) 300
EcoT14I CCWWGG 1 cut(s) 59
EcoT22I ATGCAT 1 cut(s) 322
ErhI CCWWGG 1 cut(s) 59
FaeI CATG 2 cut(s) 63, 324
FalI AAGNNNNNCTT 2 cut(s) 240, 272
FatI CATG 2 cut(s) 59, 320
FbaI TGATCA 1 cut(s) 201
Fnu4HI GCNGC 1 cut(s) 392
FokI GGATG 1 cut(s) 286
Fsp4HI GCNGC 1 cut(s) 392
FspBI CTAG 4 cut(s) 288, 344, 395, 528
GluI GCNGC 1 cut(s) 392
HaeIII GGCC 1 cut(s) 557
Hin1II CATG 2 cut(s) 63, 324
HphI GGTGA 2 cut(s) 103, 468
Hpy188I TCNGA 1 cut(s) 567
Hpy188III TCNNGA 2 cut(s) 67, 199
HpyAV CCTTC 2 cut(s) 287, 451
HpyCH4III ACNGT 2 cut(s) 163, 372
HpyCH4IV ACGT 1 cut(s) 579
HpyCH4V TGCA 6 cut(s) 38, 152, 181, 320, 391, 485
HpyF10VI GCNNNNNNNGC 1 cut(s) 35
HpySE526I ACGT 1 cut(s) 579
Hsp92II CATG 2 cut(s) 63, 324
KpnI GGTACC 1 cut(s) 432
Ksp22I TGATCA 1 cut(s) 201
Kzo9I GATC 2 cut(s) 201, 379
LmnI GCTCC 1 cut(s) 79
LpnPI CCDG 9 cut(s) 65, 204, 269, 273, 287, 314, 388, 411, 495
Lsp1109I GCAGC 1 cut(s) 403
MaeI CTAG 4 cut(s) 288, 344, 395, 528
MaeII ACGT 1 cut(s) 579
MaeIII GTNAC 3 cut(s) 40, 106, 474
MalI GATC 2 cut(s) 203, 381
MboI GATC 2 cut(s) 201, 379
MboII GAAGA 3 cut(s) 106, 248, 513
MfeI CAATTG 1 cut(s) 84
MhlI GDGCHC 1 cut(s) 328
MluCI AATT 5 cut(s) 84, 118, 130, 264, 405
MmeI TCCRAC 1 cut(s) 590
MnlI CCTC 4 cut(s) 216, 351, 447, 489
Mph1103I ATGCAT 1 cut(s) 322
MspR9I CCNGG 1 cut(s) 302
MunI CAATTG 1 cut(s) 84
MvaI CCWGG 1 cut(s) 302
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 1 cut(s) 35
NcoI CCATGG 1 cut(s) 59
NdeII GATC 2 cut(s) 201, 379
NlaIII CATG 2 cut(s) 63, 324
NlaIV GGNNCC 3 cut(s) 81, 430, 556
NmuCI GTSAC 3 cut(s) 40, 106, 474
NsiI ATGCAT 1 cut(s) 322
NspV TTCGAA 1 cut(s) 115
PflMI CCANNNNNTGG 1 cut(s) 425
PkrI GCNGC 1 cut(s) 393
Psp6I CCWGG 1 cut(s) 300
PspGI CCWGG 1 cut(s) 300
PspN4I GGNNCC 3 cut(s) 81, 430, 556
PspPI GGNCC 1 cut(s) 555
PstI CTGCAG 2 cut(s) 40, 393
RsaI GTAC 1 cut(s) 430
RsaNI GTAC 1 cut(s) 429
SatI GCNGC 1 cut(s) 392
Sau3AI GATC 2 cut(s) 201, 379
Sau96I GGNCC 1 cut(s) 555
ScrFI CCNGG 1 cut(s) 302
SduI GDGCHC 1 cut(s) 328
SetI ASST 6 cut(s) 193, 303, 396, 481, 529, 582
SfcI CTRYAG 2 cut(s) 36, 389
SfuI TTCGAA 1 cut(s) 115
Sse9I AATT 5 cut(s) 84, 118, 130, 264, 405
SsiI CCGC 2 cut(s) 11, 48
SspMI CTAG 4 cut(s) 288, 344, 395, 528
StyD4I CCNGG 1 cut(s) 300
StyI CCWWGG 1 cut(s) 59
TaaI ACNGT 2 cut(s) 163, 372
TaiI ACGT 1 cut(s) 582
TaqI TCGA 1 cut(s) 115
TaqII GACCGA 1 cut(s) 11
TasI AATT 5 cut(s) 84, 118, 130, 264, 405
TscAI CASTG 1 cut(s) 45
TseFI GTSAC 3 cut(s) 40, 106, 474
TseI GCWGC 1 cut(s) 391
Tsp45I GTSAC 3 cut(s) 40, 106, 474
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 45
Van91I CCANNNNNTGG 1 cut(s) 425
XapI RAATTY 1 cut(s) 118
XcmI CCANNNNNNNNNTGG 1 cut(s) 267
XspI CTAG 4 cut(s) 288, 344, 395, 528
Zsp2I ATGCAT 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.