Rh4BG250200

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0017813
Physical Location & Seq
Forward (+)
41951156 .. 41952818
1663 bp
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UTR
Exon/CDS
Intron
Rh4BG250200.1

Sequence Viewer

Length: 468 bp
ATGGGTGATGAAGTTCGCGGACATTTTCGGCCGGGCATTATCTGCAGTGACTTCCGCCCAGTTTCCATGGGTCAAGATGTTCAAGGAGCCAACTGTGGTGAAGATTGCCAATTATTTACTGTTGTAGCGATGGTATTGCAAAGAAAACCTGATGTCTTGATCATTATATTGCCGACATTGAGGGAAACTTCAAAGTATCAAGGACAAGATAACCTTCCAGTAATTGTATGGATGATAGTTCAAGCTAGTGAAGGAGACCTGGCTTTGGCGTTGTATGCATCGGCACACAATCTTTTTAAAAAAGAATGGTGCTATCTGGATCAAGAAGTTGATATTTCAGATCTAACAGTTTCAGACTGTTGGAAACGACTTCCTGACCATATTGAGGAGAAAAACTTGTGTTCCCGCAACAGAAAAATGACTATGATTGTGGTCTCTTTGTACTCTTGTTTATGGAACGCTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

155

Amino Acids

17.62

Weight (kDa)

5.12

Isoelectric Point (pI)

44.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017813)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0017851
rosa_laevigata RLG00000020318 RLG00000030040
rosa_rugosa Rorug02G0253100 Rorug03G0048400
rosa_samantha Rh1BG268600 Rh1DG298700 Rh4AG245000 Rh4BG250200 Rh6BG057500 Rh6DG335800
rosa_wichuraiana Rw2G043450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 18
AciI CCGC 3 cut(s) 18, 55, 406
AclWI GGATC 1 cut(s) 327
AcoI YGGCCR 1 cut(s) 29
AfaI GTAC 1 cut(s) 443
AfiI CCNNNNNNNGG 2 cut(s) 265, 385
AgsI TTSAA 3 cut(s) 83, 192, 242
AjnI CCWGG 1 cut(s) 258
AluBI AGCT 1 cut(s) 245
AluI AGCT 1 cut(s) 245
Alw26I GTCTC 2 cut(s) 249, 439
AlwI GGATC 1 cut(s) 327
AoxI GGCC 1 cut(s) 29
AsuC2I CCSGG 1 cut(s) 33
AsuHPI GGTGA 2 cut(s) 17, 110
BccI CCATC 1 cut(s) 124
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciT130I CCWGG 1 cut(s) 260
BclI TGATCA 1 cut(s) 159
BcnI CCSGG 1 cut(s) 33
BcoDI GTCTC 2 cut(s) 249, 439
BfaI CTAG 1 cut(s) 246
BfmI CTRYAG 1 cut(s) 43
BglII AGATCT 1 cut(s) 340
Bme1390I CCNGG 2 cut(s) 33, 260
BmiI GGNNCC 1 cut(s) 88
BmrFI CCNGG 2 cut(s) 33, 260
BmrI ACTGGG 1 cut(s) 53
BmsI GCATC 1 cut(s) 287
BmuI ACTGGG 1 cut(s) 53
BpuMI CCSGG 1 cut(s) 33
BsaI GGTCTC 2 cut(s) 249, 439
BsaJI CCNNGG 1 cut(s) 66
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 385
Bse1I ACTGG 2 cut(s) 59, 218
BseBI CCWGG 1 cut(s) 260
BseDI CCNNGG 1 cut(s) 66
BseGI GGATG 1 cut(s) 237
BseLI CCNNNNNNNGG 2 cut(s) 265, 385
BseNI ACTGG 2 cut(s) 59, 218
BseRI GAGGAG 1 cut(s) 401
BseX3I CGGCCG 1 cut(s) 29
Bsh1236I CGCG 1 cut(s) 18
Bsh1285I CGRYCG 1 cut(s) 32
BshFI GGCC 1 cut(s) 31
BsiEI CGRYCG 1 cut(s) 32
BsiSI CCGG 1 cut(s) 32
BslI CCNNNNNNNGG 2 cut(s) 265, 385
BsmAI GTCTC 2 cut(s) 249, 439
BsnI GGCC 1 cut(s) 31
Bso31I GGTCTC 2 cut(s) 249, 439
Bsp143I GATC 3 cut(s) 159, 319, 340
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 3 cut(s) 18, 55, 406
BspANI GGCC 1 cut(s) 31
BspFNI CGCG 1 cut(s) 18
BspLI GGNNCC 1 cut(s) 88
BspMAI CTGCAG 1 cut(s) 47
BspPI GGATC 1 cut(s) 327
BspTNI GGTCTC 2 cut(s) 249, 439
BsrI ACTGG 2 cut(s) 59, 218
BssECI CCNNGG 1 cut(s) 66
BssMI GATC 3 cut(s) 159, 319, 340
BssT1I CCWWGG 1 cut(s) 66
Bst2UI CCWGG 1 cut(s) 260
Bst4CI ACNGT 4 cut(s) 95, 121, 349, 359
BstAPI GCANNNNNTGC 1 cut(s) 42
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 1 cut(s) 237
BstFNI CGCG 1 cut(s) 18
BstKTI GATC 3 cut(s) 162, 322, 343
BstMAI GTCTC 2 cut(s) 249, 439
BstMBI GATC 3 cut(s) 159, 319, 340
BstMCI CGRYCG 1 cut(s) 32
BstMWI GCNNNNNNNGC 2 cut(s) 42, 275
BstNI CCWGG 1 cut(s) 260
BstSCI CCNGG 2 cut(s) 31, 258
BstSFI CTRYAG 1 cut(s) 43
BstUI CGCG 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
BstZI CGGCCG 1 cut(s) 29
BsuRI GGCC 1 cut(s) 31
BtgI CCRYGG 1 cut(s) 66
BtgZI GCGATG 1 cut(s) 143
BtsCI GGATG 1 cut(s) 237
BtsI GCAGTG 1 cut(s) 52
BtsIMutI CAGTG 1 cut(s) 52
Csp6I GTAC 1 cut(s) 442
CviAII CATG 1 cut(s) 67
CviJI RGCY 4 cut(s) 31, 89, 245, 263
CviKI_1 RGCY 4 cut(s) 31, 89, 245, 263
CviQI GTAC 1 cut(s) 442
DpnI GATC 3 cut(s) 161, 321, 342
DpnII GATC 3 cut(s) 159, 319, 340
DraI TTTAAA 1 cut(s) 298
EaeI YGGCCR 1 cut(s) 29
EagI CGGCCG 1 cut(s) 29
EciI GGCGGA 1 cut(s) 44
EclXI CGGCCG 1 cut(s) 29
Eco130I CCWWGG 1 cut(s) 66
Eco31I GGTCTC 2 cut(s) 249, 439
Eco52I CGGCCG 1 cut(s) 29
EcoRII CCWGG 1 cut(s) 258
EcoT14I CCWWGG 1 cut(s) 66
EcoT22I ATGCAT 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 66
FaeI CATG 1 cut(s) 70
FaiI YATR 8 cut(s) 68, 167, 229, 276, 381, 425, 454, 466
FalI AAGNNNNNCTT 2 cut(s) 198, 230
FatI CATG 1 cut(s) 66
FauI CCCGC 1 cut(s) 413
FbaI TGATCA 1 cut(s) 159
FokI GGATG 1 cut(s) 244
FspBI CTAG 1 cut(s) 246
HaeIII GGCC 1 cut(s) 31
HapII CCGG 1 cut(s) 32
Hin1II CATG 1 cut(s) 70
HpaII CCGG 1 cut(s) 32
HphI GGTGA 2 cut(s) 17, 110
Hpy188I TCNGA 2 cut(s) 340, 355
Hpy188III TCNNGA 5 cut(s) 74, 157, 317, 323, 374
HpyAV CCTTC 2 cut(s) 224, 245
HpyCH4III ACNGT 4 cut(s) 95, 121, 349, 359
HpyCH4V TGCA 3 cut(s) 45, 139, 278
HpyF10VI GCNNNNNNNGC 2 cut(s) 42, 275
Hsp92II CATG 1 cut(s) 70
Ksp22I TGATCA 1 cut(s) 159
Kzo9I GATC 3 cut(s) 159, 319, 340
LmnI GCTCC 1 cut(s) 86
LpnPI CCDG 8 cut(s) 45, 72, 162, 231, 245, 272, 302, 387
LweI GCATC 1 cut(s) 287
MaeI CTAG 1 cut(s) 246
MaeIII GTNAC 1 cut(s) 47
MalI GATC 3 cut(s) 161, 321, 342
MboI GATC 3 cut(s) 159, 319, 340
MboII GAAGA 1 cut(s) 113
MflI RGATCY 1 cut(s) 340
MluCI AATT 2 cut(s) 110, 222
MmeI TCCRAC 1 cut(s) 341
MnlI CCTC 2 cut(s) 174, 379
Mph1103I ATGCAT 1 cut(s) 280
MseI TTAA 1 cut(s) 297
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 2 cut(s) 33, 260
MvaI CCWGG 1 cut(s) 260
MvnI CGCG 1 cut(s) 18
MwoI GCNNNNNNNGC 2 cut(s) 42, 275
NciI CCSGG 1 cut(s) 33
NcoI CCATGG 1 cut(s) 66
NdeII GATC 3 cut(s) 159, 319, 340
NlaIII CATG 1 cut(s) 70
NlaIV GGNNCC 1 cut(s) 88
NmuCI GTSAC 1 cut(s) 47
NsiI ATGCAT 1 cut(s) 280
Psp6I CCWGG 1 cut(s) 258
PspGI CCWGG 1 cut(s) 258
PspN4I GGNNCC 1 cut(s) 88
PstI CTGCAG 1 cut(s) 47
PsuI RGATCY 1 cut(s) 340
RsaI GTAC 1 cut(s) 443
RsaNI GTAC 1 cut(s) 442
SaqAI TTAA 1 cut(s) 297
Sau3AI GATC 3 cut(s) 159, 319, 340
ScrFI CCNGG 2 cut(s) 33, 260
SetI ASST 4 cut(s) 151, 216, 247, 261
SfaNI GCATC 1 cut(s) 287
SfcI CTRYAG 1 cut(s) 43
Sse9I AATT 2 cut(s) 110, 222
SsiI CCGC 3 cut(s) 18, 55, 406
SspMI CTAG 1 cut(s) 246
StyD4I CCNGG 2 cut(s) 31, 258
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 4 cut(s) 95, 121, 349, 359
TasI AATT 2 cut(s) 110, 222
TatI WGTACW 1 cut(s) 441
Tru1I TTAA 1 cut(s) 297
Tru9I TTAA 1 cut(s) 297
TscAI CASTG 1 cut(s) 52
TseFI GTSAC 1 cut(s) 47
Tsp45I GTSAC 1 cut(s) 47
TspDTI ATGAA 1 cut(s) 24
TspRI CASTG 1 cut(s) 52
XcmI CCANNNNNNNNNTGG 1 cut(s) 225
XspI CTAG 1 cut(s) 246
Zsp2I ATGCAT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.