Rorug02G0253100

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0010935
Physical Location & Seq
Forward (+)
26510629 .. 26511691
1063 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0253100.1

Sequence Viewer

Length: 327 bp
ATGAACGACCTGTTTTCGGGCTCCTTCTCCAGGTTCCGCAGCGAACCCGCCAACAACGACAAGCACGTCATCGAGATGTCATTGGCGACGGCGTCCGGCACCGGAGTCAACCTCGACAAGTTCTTCGGCGACGTCGAGTCCGTCAAGGACGAGCTCAAGGAGCTGGAGCGCATCCTCCAGAACCTCCAGTCTGCTCACGAGCAGAGCAAGACTCTCCACAACGCCAACACCGTTAAGGACCTCCGATCTCGCATGGACGCCGACCTCCGAGCTGTAGAAATGATGAAATTTGTGATCTTTGATTGTGGGTCTTGTGTGTTTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

108

Amino Acids

12.17

Weight (kDa)

5.29

Isoelectric Point (pI)

56.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Syntaxin PF00804 37 - 93 3.2e-11 Syntaxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010935)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10200 AT3G10200 AT5G04060
fragaria_vesca FvH4_6g25080 FvH4_6g25080
malus_domestica MD14G1083600.v1.1
prunus_persica Prupe.7G047000_v2.0.a1
pyrus_communis pycom14g07260
rosa_chinensis RchiOBHm_Chr2g0124841
rosa_laevigata RLG00000018828
rosa_multiflora Rmu_co8443503.1_g000001 Rmu_sc0005075.1_g000002
rosa_roxburghii Rroxscaffold_2G00119610 Rroxscaffold_4G00311160
rosa_rugosa Rorug02G0253100
rosa_samantha Rh2AG312600 Rh2BG320800 Rh2CG300900 Rh2DG338100
rosa_wichuraiana Rw2G025250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 65
AatII GACGTC 1 cut(s) 135
AccB1I GGYRCC 1 cut(s) 98
AciI CCGC 2 cut(s) 37, 48
AcsI RAATTY 1 cut(s) 287
AcyI GRCGYC 3 cut(s) 92, 132, 258
AfiI CCNNNNNNNGG 2 cut(s) 16, 30
AhdI GACNNNNNGTC 1 cut(s) 136
AjiI CACGTC 1 cut(s) 67
AjnI CCWGG 1 cut(s) 29
AluBI AGCT 3 cut(s) 154, 163, 272
AluI AGCT 3 cut(s) 154, 163, 272
Alw21I GWGCWC 1 cut(s) 156
ApeKI GCWGC 1 cut(s) 39
ApoI RAATTY 1 cut(s) 287
ArsI GACNNNNNNTTYG 2 cut(s) 107, 139
AspLEI GCGC 1 cut(s) 171
AspS9I GGNCC 1 cut(s) 238
AvaII GGWCC 1 cut(s) 238
BanI GGYRCC 1 cut(s) 98
BanII GRGCYC 2 cut(s) 23, 156
BauI CACGAG 1 cut(s) 197
Bbv12I GWGCWC 1 cut(s) 156
BbvI GCAGC 1 cut(s) 51
BceAI ACGGC 1 cut(s) 105
BciT130I CCWGG 1 cut(s) 31
BfmI CTRYAG 1 cut(s) 273
BisI GCNGC 1 cut(s) 40
BlsI GCNGC 1 cut(s) 41
Bme1390I CCNGG 1 cut(s) 31
Bme18I GGWCC 1 cut(s) 238
BmeRI GACNNNNNGTC 1 cut(s) 136
BmgBI CACGTC 1 cut(s) 67
BmgT120I GGNCC 1 cut(s) 238
BmiI GGNNCC 3 cut(s) 22, 35, 100
BmrFI CCNGG 1 cut(s) 31
BmsI GCATC 1 cut(s) 180
BplI GAGNNNNNCTC 4 cut(s) 96, 128, 196, 228
BpmI CTGGAG 4 cut(s) 13, 161, 170, 185
BpuEI CTTGAG 1 cut(s) 140
BsaHI GRCGYC 3 cut(s) 92, 132, 258
BsaWI WCCGGW 1 cut(s) 101
Bsc4I CCNNNNNNNGG 2 cut(s) 16, 30
Bse1I ACTGG 1 cut(s) 187
BseBI CCWGG 1 cut(s) 31
BseGI GGATG 1 cut(s) 171
BseLI CCNNNNNNNGG 2 cut(s) 16, 30
BseNI ACTGG 1 cut(s) 187
BseXI GCAGC 1 cut(s) 51
BshNI GGYRCC 1 cut(s) 98
BsiHKAI GWGCWC 1 cut(s) 156
BsiSI CCGG 2 cut(s) 96, 102
BslI CCNNNNNNNGG 2 cut(s) 16, 30
Bsp1286I GDGCHC 2 cut(s) 23, 156
Bsp143I GATC 2 cut(s) 245, 294
BspACI CCGC 2 cut(s) 37, 48
BspLI GGNNCC 3 cut(s) 22, 35, 100
BspT107I GGYRCC 1 cut(s) 98
BsrI ACTGG 1 cut(s) 187
BssMI GATC 2 cut(s) 245, 294
BssNI GRCGYC 3 cut(s) 92, 132, 258
BssSI CACGAG 1 cut(s) 197
Bst2BI CACGAG 1 cut(s) 197
Bst2UI CCWGG 1 cut(s) 31
Bst4CI ACNGT 1 cut(s) 232
BstACI GRCGYC 3 cut(s) 92, 132, 258
BstENI CCTNNNNNAGG 1 cut(s) 28
BstF5I GGATG 1 cut(s) 171
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 2 cut(s) 248, 297
BstMBI GATC 2 cut(s) 245, 294
BstMWI GCNNNNNNNGC 1 cut(s) 160
BstNI CCWGG 1 cut(s) 31
BstSCI CCNGG 1 cut(s) 29
BstSFI CTRYAG 1 cut(s) 273
BstV1I GCAGC 1 cut(s) 51
BtrI CACGTC 1 cut(s) 67
BtsCI GGATG 1 cut(s) 171
CfoI GCGC 1 cut(s) 171
Cfr13I GGNCC 1 cut(s) 238
CseI GACGC 2 cut(s) 81, 266
CviAII CATG 1 cut(s) 253
CviJI RGCY 4 cut(s) 21, 154, 163, 272
CviKI_1 RGCY 4 cut(s) 21, 154, 163, 272
DpnI GATC 2 cut(s) 247, 296
DpnII GATC 2 cut(s) 245, 294
DrdI GACNNNNNNGTC 1 cut(s) 65
DriI GACNNNNNGTC 1 cut(s) 136
DseDI GACNNNNNNGTC 1 cut(s) 65
Eam1105I GACNNNNNGTC 1 cut(s) 136
Ecl136II GAGCTC 1 cut(s) 154
Eco24I GRGCYC 2 cut(s) 23, 156
Eco47I GGWCC 1 cut(s) 238
Eco53kI GAGCTC 1 cut(s) 154
EcoICRI GAGCTC 1 cut(s) 154
EcoNI CCTNNNNNAGG 1 cut(s) 28
EcoO109I RGGNCCY 1 cut(s) 238
EcoRII CCWGG 1 cut(s) 29
EcoT38I GRGCYC 2 cut(s) 23, 156
FaeI CATG 1 cut(s) 256
FaiI YATR 1 cut(s) 254
FatI CATG 1 cut(s) 252
FauI CCCGC 1 cut(s) 55
Fnu4HI GCNGC 1 cut(s) 40
FokI GGATG 1 cut(s) 158
FriOI GRGCYC 2 cut(s) 23, 156
Fsp4HI GCNGC 1 cut(s) 40
GlaI GCGC 1 cut(s) 170
GluI GCNGC 1 cut(s) 40
GsuI CTGGAG 4 cut(s) 13, 161, 170, 185
HapII CCGG 2 cut(s) 96, 102
HgaI GACGC 2 cut(s) 81, 266
HhaI GCGC 1 cut(s) 171
Hin1I GRCGYC 3 cut(s) 92, 132, 258
Hin1II CATG 1 cut(s) 256
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HinfI GANTC 3 cut(s) 105, 137, 211
HpaII CCGG 2 cut(s) 96, 102
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 2 cut(s) 245, 269
Hpy188III TCNNGA 3 cut(s) 73, 178, 197
Hpy8I GTNNAC 1 cut(s) 109
Hpy99I CGWCG 3 cut(s) 91, 134, 137
HpyAV CCTTC 1 cut(s) 34
HpyCH4III ACNGT 1 cut(s) 232
HpyCH4IV ACGT 2 cut(s) 66, 132
HpyF10VI GCNNNNNNNGC 1 cut(s) 160
HpySE526I ACGT 2 cut(s) 66, 132
Hsp92I GRCGYC 3 cut(s) 92, 132, 258
Hsp92II CATG 1 cut(s) 256
HspAI GCGC 1 cut(s) 169
Kzo9I GATC 2 cut(s) 245, 294
LmnI GCTCC 3 cut(s) 26, 160, 166
LpnPI CCDG 8 cut(s) 16, 23, 43, 109, 115, 149, 191, 200
Lsp1109I GCAGC 1 cut(s) 51
LweI GCATC 1 cut(s) 180
MaeII ACGT 2 cut(s) 66, 132
MalI GATC 2 cut(s) 247, 296
MboI GATC 2 cut(s) 245, 294
MboII GAAGA 1 cut(s) 115
MhlI GDGCHC 2 cut(s) 23, 156
MluCI AATT 1 cut(s) 287
MlyI GAGTC 3 cut(s) 114, 146, 205
MnlI CCTC 5 cut(s) 122, 185, 194, 251, 275
MseI TTAA 1 cut(s) 234
MslI CAYNNNNRTG 1 cut(s) 74
MspI CCGG 2 cut(s) 96, 102
MspR9I CCNGG 1 cut(s) 31
MvaI CCWGG 1 cut(s) 31
MwoI GCNNNNNNNGC 1 cut(s) 160
NdeII GATC 2 cut(s) 245, 294
NlaIII CATG 1 cut(s) 256
NlaIV GGNNCC 3 cut(s) 22, 35, 100
PcsI WCGNNNNNNNCGW 4 cut(s) 63, 132, 138, 228
PflFI GACNNNGTC 1 cut(s) 91
PkrI GCNGC 1 cut(s) 41
PleI GAGTC 3 cut(s) 113, 145, 205
PpsI GAGTC 3 cut(s) 113, 145, 205
PpuMI RGGWCCY 1 cut(s) 238
Psp124BI GAGCTC 1 cut(s) 156
Psp5II RGGWCCY 1 cut(s) 238
Psp6I CCWGG 1 cut(s) 29
PspGI CCWGG 1 cut(s) 29
PspN4I GGNNCC 3 cut(s) 22, 35, 100
PspPI GGNCC 1 cut(s) 238
PspPPI RGGWCCY 1 cut(s) 238
PsyI GACNNNGTC 1 cut(s) 91
RseI CAYNNNNRTG 1 cut(s) 74
SacI GAGCTC 1 cut(s) 156
SaqAI TTAA 1 cut(s) 234
SatI GCNGC 1 cut(s) 40
Sau3AI GATC 2 cut(s) 245, 294
Sau96I GGNCC 1 cut(s) 238
SchI GAGTC 3 cut(s) 114, 146, 205
ScrFI CCNGG 1 cut(s) 31
SduI GDGCHC 2 cut(s) 23, 156
SfaNI GCATC 1 cut(s) 180
SfcI CTRYAG 1 cut(s) 273
SinI GGWCC 1 cut(s) 238
SmiMI CAYNNNNRTG 1 cut(s) 74
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 1 cut(s) 287
SsiI CCGC 2 cut(s) 37, 48
SstI GAGCTC 1 cut(s) 156
StyD4I CCNGG 1 cut(s) 29
TaaI ACNGT 1 cut(s) 232
TaiI ACGT 2 cut(s) 69, 135
TaqI TCGA 3 cut(s) 72, 114, 135
TasI AATT 1 cut(s) 287
Tru1I TTAA 1 cut(s) 234
Tru9I TTAA 1 cut(s) 234
TseI GCWGC 1 cut(s) 39
TspDTI ATGAA 2 cut(s) 17, 299
TspGWI ACGGA 1 cut(s) 130
Tth111I GACNNNGTC 1 cut(s) 91
VpaK11BI GGWCC 1 cut(s) 238
XagI CCTNNNNNAGG 1 cut(s) 28
XapI RAATTY 1 cut(s) 287
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.