pycom14g07260

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010935
Physical Location & Seq
Forward (+)
7284947 .. 7289060
4114 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g07260.4

Sequence Viewer

Length: 1794 bp
ATGGGAGGTGGGCAGGCGTTTGAATGGAAATTGGGGCAGATGATAATGGTGGGTCTGCTGCTGATGGTTGGTTGCTTCTATGCTGGAACTCTACATGCCAACAATGCTACCATTTATGCATCTCAGCTAAGCTCCTCCAGTTCTCCTCCGTCCCCTGGTACTTCAACATTTACGAATACAATTGCTCTTACTTACAGAAAAACACCACCGTTGATCCCAGAGACGGGTATCAATGTATGCCCATTGAATTTAAATGAGTACATTCCCTGCCATGATGTTGCTTATGTAAAAACTTTGCTTCCAAGCTTGGACACCAATAGAAGAGAAGAACTAGAGAGGCACTGCCCTCCCATTGAAAAGCGCTTGTTTTGCTTGGTTCCACCACCAAAAGATTATAAGAAACCAATAAGATGGCCAACCAGCAGGGATTATGTGTGGCGAAGCAATGTGAATCATACACATCTTGCTGAAGTCAAAGGAGGACAAAATTGGGTGCATCCAAAGGATCAATTCTGGTGGTTCCCTGGTGGTGGTACTCATTTTAAGCACGGTGCACCCGAGTACATTCAAAGATTGGGAGATATGGTAACAAATGGAACCGGTGATCTACGTTCTGCAGGAATTAGTCAAGTCTTGGATGTCGGTTGTGGGGTTGCCAGTTTTTCTGCTTTTCTTCTTGCCTTGGATATACAAACAATGTCCTTTGCTCCAAAGGATGGTCATGAGAATCAAATTCAATTTGCTTTAGAGAGGGGCATTGGTGCAATGATTTCTGCTATAGCAACAAAACAAATGCCATATCCCTCTAGCTCTTTTGAAATGGTTCATTGTTCTAGGTGTCGAGTTGATTGGCATGAAAATGATGGCATTTTGCTAAAAGAATTGAATCGCCTTTTGCGTTCTAATGGGTACTTTGTCTATTCAGCCCCACCTGCTTATAAGAAGGATAAAGATTATCGTATAATTTGGGAGAAGTTAATGAATCTGACTTCAGCAATGTGCTGGAAACTCATTGCTCGAAAAGTTCAGACTGCGATCTGGATTAAACAAGATGATCTGTCATGCCTCCAGCACAATTCTGAACAGAAGCTTGTAGATTTATGTGATGCTGGGGATGATTCCAAGTCATCATGGAATACACAGCTGAGGAATTGTATACAAGTTAGGGGTGCACAATCAGATTCTCCAAAACTTGCTTCTAGACCTGAGCGCCTTTCAGTATACTCAGAGAGTCTTAGCAGAATAGGTGTTACTCAAGAGGTATTTTCTTTAGAGACTGCCTTTTGGCAAGATCAAGTTCAGCGTTACTGGAGGTTGCTGAATGTCAATAAGACAGAAATACGAAATGTCATGGACATGAATGCATTTTGTGGTGGATTTGCTGTGGCTTTAAATGACTCGCCTACATGGGTGATGAATATAGTTCCTGCAAGCATGAGCAATACATTGTCTGCCATTTACAATCGTGGTCTGATTGGTGCTTTCCATGATTGGTGCGAACCATTTTCCACTTATCCACGTACCTATGATCTGTTACATGCCAACCACCTCTTCTCTCATTATAAGAACCTGGGGCAACAAGGTTGCGTATTGGCGGATATCCTTCTAGAGATGGACAGGATGATCAGACCTCAGGGATTCATTATCATTAGGGATGTGGAAACCATTACAGCAAGGATCCAGGATCTTGCTCCAAAATTTTTATGGGAGGTTGAATCGCATATGCTGGAAAACATAGAGAAGAAACCTGAAACTGTGTTAATTTGTAGGAAGAAGTTTTGGGCAATCGTCTGA

Protein Analysis

598

Amino Acids

67.74

Weight (kDa)

8.06

Isoelectric Point (pI)

42.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010935)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10200 AT3G10200 AT5G04060
fragaria_vesca FvH4_6g25080 FvH4_6g25080
malus_domestica MD14G1083600.v1.1
prunus_persica Prupe.7G047000_v2.0.a1
pyrus_communis pycom14g07260
rosa_chinensis RchiOBHm_Chr2g0124841
rosa_laevigata RLG00000018828
rosa_multiflora Rmu_co8443503.1_g000001 Rmu_sc0005075.1_g000002
rosa_roxburghii Rroxscaffold_2G00119610 Rroxscaffold_4G00311160
rosa_rugosa Rorug02G0253100
rosa_samantha Rh2AG312600 Rh2BG320800 Rh2CG300900 Rh2DG338100
rosa_wichuraiana Rw2G025250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 396, 939, 1563
AarI CACCTGC 1 cut(s) 940
Acc36I ACCTGC 1 cut(s) 940
AccB7I CCANNNNNTGG 1 cut(s) 716
AccI GTMKAC 2 cut(s) 1156, 1221
AciI CCGC 1 cut(s) 1595
AclWI GGATC 5 cut(s) 208, 513, 1672, 1685, 1692
AcoI YGGCCR 1 cut(s) 413
AcsI RAATTY 3 cut(s) 247, 732, 1697
AcuI CTGAAG 2 cut(s) 489, 975
AfaI GTAC 6 cut(s) 160, 260, 535, 563, 911, 1522
AfeI AGCGCT 1 cut(s) 362
AfiI CCNNNNNNNGG 5 cut(s) 155, 223, 224, 530, 716
AgeI ACCGGT 1 cut(s) 599
AgsI TTSAA 9 cut(s) 23, 165, 247, 356, 569, 737, 818, 886, 1715
AjnI CCWGG 4 cut(s) 154, 523, 1569, 1680
AjuI GAANNNNNNNTTGG 4 cut(s) 1180, 1212, 1535, 1567
AluBI AGCT 6 cut(s) 127, 132, 306, 810, 1090, 1144
AluI AGCT 6 cut(s) 127, 132, 306, 810, 1090, 1144
Alw21I GWGCWC 2 cut(s) 556, 1174
Alw26I GTCTC 2 cut(s) 215, 1268
Alw44I GTGCAC 2 cut(s) 552, 1170
AlwI GGATC 5 cut(s) 208, 513, 1672, 1685, 1692
Ama87I CYCGRG 1 cut(s) 557
Aor51HI AGCGCT 1 cut(s) 362
AoxI GGCC 1 cut(s) 413
ApaLI GTGCAC 2 cut(s) 552, 1170
ApeKI GCWGC 1 cut(s) 58
ApoI RAATTY 3 cut(s) 247, 732, 1697
AsiGI ACCGGT 1 cut(s) 599
Asp700I GAANNNNTTC 1 cut(s) 822
AspLEI GCGC 2 cut(s) 363, 1212
AsuHPI GGTGA 2 cut(s) 614, 1423
AvaI CYCGRG 1 cut(s) 557
AxyI CCTNAGG 1 cut(s) 1632
BaeGI GKGCMC 2 cut(s) 556, 1174
BalI TGGCCA 1 cut(s) 415
BamHI GGATCC 1 cut(s) 1677
Bbv12I GWGCWC 2 cut(s) 556, 1174
BbvCI CCTCAGC 1 cut(s) 1145
BbvI GCAGC 1 cut(s) 45
BccI CCATC 5 cut(s) 58, 405, 710, 857, 1606
BciT130I CCWGG 4 cut(s) 156, 525, 1571, 1682
BclI TGATCA 1 cut(s) 1623
BcoDI GTCTC 2 cut(s) 215, 1268
BfaI CTAG 5 cut(s) 332, 807, 834, 1200, 1607
BfmI CTRYAG 2 cut(s) 615, 777
BfoI RGCGCY 2 cut(s) 364, 1213
BfuAI ACCTGC 1 cut(s) 940
BisI GCNGC 1 cut(s) 59
BlpI GCTNAGC 1 cut(s) 128
BlsI GCNGC 1 cut(s) 60
Bme1390I CCNGG 4 cut(s) 156, 525, 1571, 1682
BmeT110I CYCGRG 1 cut(s) 557
BmiI GGNNCC 4 cut(s) 378, 521, 598, 1679
BmrFI CCNGG 4 cut(s) 156, 525, 1571, 1682
BmsI GCATC 3 cut(s) 128, 505, 1096
BpmI CTGGAG 3 cut(s) 121, 1052, 1330
Bpu10I CCTNAGC 2 cut(s) 1145, 1206
Bpu1102I GCTNAGC 1 cut(s) 128
BpuEI CTTGAG 1 cut(s) 1239
BsaAI YACGTR 1 cut(s) 1520
BsaJI CCNNGG 4 cut(s) 154, 523, 681, 1570
BsaWI WCCGGW 1 cut(s) 599
BsaXI ACNNNNNCTCC 2 cut(s) 570, 600
Bsc4I CCNNNNNNNGG 5 cut(s) 155, 223, 224, 530, 716
Bse118I RCCGGY 1 cut(s) 599
Bse1I ACTGG 3 cut(s) 138, 657, 1313
Bse21I CCTNAGG 1 cut(s) 1632
Bse3DI GCAATG 4 cut(s) 451, 771, 1002, 1011
BseBI CCWGG 4 cut(s) 156, 525, 1571, 1682
BseDI CCNNGG 4 cut(s) 154, 523, 681, 1570
BseGI GGATG 6 cut(s) 496, 643, 721, 1120, 1626, 1660
BseLI CCNNNNNNNGG 5 cut(s) 155, 223, 224, 530, 716
BseMI GCAATG 4 cut(s) 451, 771, 1002, 1011
BseMII CTCAG 5 cut(s) 137, 1136, 1197, 1239, 1646
BseNI ACTGG 3 cut(s) 138, 657, 1313
BseRI GAGGAG 2 cut(s) 124, 135
BseSI GKGCMC 2 cut(s) 556, 1174
BseXI GCAGC 1 cut(s) 45
BseYI CCCAGC 1 cut(s) 1109
BshFI GGCC 1 cut(s) 415
BshTI ACCGGT 1 cut(s) 599
BsiHKAI GWGCWC 2 cut(s) 556, 1174
BsiHKCI CYCGRG 1 cut(s) 557
BsiSI CCGG 1 cut(s) 600
BslFI GGGAC 1 cut(s) 136
BslI CCNNNNNNNGG 5 cut(s) 155, 223, 224, 530, 716
BsmAI GTCTC 2 cut(s) 215, 1268
BsmBI CGTCTC 1 cut(s) 215
BsmFI GGGAC 1 cut(s) 136
BsmI GAATGC 1 cut(s) 1366
BsnI GGCC 1 cut(s) 415
BsoBI CYCGRG 1 cut(s) 557
Bsp1286I GDGCHC 2 cut(s) 556, 1174
Bsp1720I GCTNAGC 1 cut(s) 128
BspACI CCGC 1 cut(s) 1595
BspANI GGCC 1 cut(s) 415
BspCNI CTCAG 5 cut(s) 136, 1137, 1198, 1238, 1645
BspHI TCATGA 1 cut(s) 721
BspLI GGNNCC 4 cut(s) 378, 521, 598, 1679
BspMAI CTGCAG 1 cut(s) 619
BspMI ACCTGC 1 cut(s) 940
BspPI GGATC 5 cut(s) 208, 513, 1672, 1685, 1692
BsrDI GCAATG 4 cut(s) 451, 771, 1002, 1011
BsrFI RCCGGY 1 cut(s) 599
BsrI ACTGG 3 cut(s) 138, 657, 1313
BssAI RCCGGY 1 cut(s) 599
BssECI CCNNGG 4 cut(s) 154, 523, 681, 1570
BssNAI GTATAC 2 cut(s) 1157, 1222
BssT1I CCWWGG 1 cut(s) 681
Bst1107I GTATAC 2 cut(s) 1157, 1222
Bst2UI CCWGG 4 cut(s) 156, 525, 1571, 1682
Bst4CI ACNGT 3 cut(s) 210, 551, 1756
Bst6I CTCTTC 2 cut(s) 316, 1556
BstBAI YACGTR 1 cut(s) 1520
BstC8I GCNNGC 2 cut(s) 15, 1432
BstDEI CTNAG 7 cut(s) 123, 128, 1145, 1206, 1225, 1235, 1632
BstF5I GGATG 6 cut(s) 496, 643, 721, 1120, 1626, 1660
BstH2I RGCGCY 2 cut(s) 364, 1213
BstHHI GCGC 2 cut(s) 363, 1212
BstMAI GTCTC 2 cut(s) 215, 1268
BstMWI GCNNNNNNNGC 3 cut(s) 104, 369, 932
BstNI CCWGG 4 cut(s) 156, 525, 1571, 1682
BstNSI RCATGY 2 cut(s) 98, 1541
BstSCI CCNGG 4 cut(s) 154, 523, 1569, 1680
BstSFI CTRYAG 2 cut(s) 615, 777
BstSLI GKGCMC 2 cut(s) 556, 1174
BstV1I GCAGC 1 cut(s) 45
BstX2I RGATCY 2 cut(s) 1677, 1684
BstXI CCANNNNNNTGG 1 cut(s) 411
BstYI RGATCY 2 cut(s) 1677, 1684
BstZ17I GTATAC 2 cut(s) 1157, 1222
Bsu36I CCTNAGG 1 cut(s) 1632
BsuRI GGCC 1 cut(s) 415
BtsCI GGATG 6 cut(s) 496, 643, 721, 1120, 1626, 1660
BtsI GCAGTG 1 cut(s) 340
BtsIMutI CAGTG 1 cut(s) 340
BveI ACCTGC 1 cut(s) 940
Cac8I GCNNGC 2 cut(s) 15, 1432
CciI TCATGA 1 cut(s) 721
CfoI GCGC 2 cut(s) 363, 1212
Cfr10I RCCGGY 1 cut(s) 599
Csp6I GTAC 6 cut(s) 159, 259, 534, 562, 910, 1521
CspAI ACCGGT 1 cut(s) 599
CspCI CAANNNNNGTGG 2 cut(s) 497, 532
CviJI RGCY 9 cut(s) 127, 132, 306, 415, 810, 926, 1090, 1144, 1388
CviKI_1 RGCY 9 cut(s) 127, 132, 306, 415, 810, 926, 1090, 1144, 1388
CviQI GTAC 6 cut(s) 159, 259, 534, 562, 910, 1521
DdeI CTNAG 7 cut(s) 123, 128, 1145, 1206, 1225, 1235, 1632
DraI TTTAAA 2 cut(s) 252, 1392
EaeI YGGCCR 1 cut(s) 413
Eam1104I CTCTTC 2 cut(s) 316, 1556
EarI CTCTTC 2 cut(s) 316, 1556
EciI GGCGGA 1 cut(s) 1610
Eco130I CCWWGG 1 cut(s) 681
Eco32I GATATC 1 cut(s) 1600
Eco47III AGCGCT 1 cut(s) 362
Eco57I CTGAAG 2 cut(s) 489, 975
Eco81I CCTNAGG 1 cut(s) 1632
Eco88I CYCGRG 1 cut(s) 557
EcoRII CCWGG 4 cut(s) 154, 523, 1569, 1680
EcoRV GATATC 1 cut(s) 1600
EcoT14I CCWWGG 1 cut(s) 681
EcoT22I ATGCAT 2 cut(s) 121, 1366
ErhI CCWWGG 1 cut(s) 681
Esp3I CGTCTC 1 cut(s) 215
FaqI GGGAC 1 cut(s) 136
FauNDI CATATG 1 cut(s) 1722
FbaI TGATCA 1 cut(s) 1623
FblI GTMKAC 2 cut(s) 1156, 1221
Fnu4HI GCNGC 1 cut(s) 59
FokI GGATG 6 cut(s) 483, 650, 728, 1127, 1633, 1667
Fsp4HI GCNGC 1 cut(s) 59
FspBI CTAG 5 cut(s) 332, 807, 834, 1200, 1607
GlaI GCGC 2 cut(s) 362, 1211
GluI GCNGC 1 cut(s) 59
GsaI CCCAGC 1 cut(s) 1113
GsuI CTGGAG 3 cut(s) 121, 1052, 1330
HaeII RGCGCY 2 cut(s) 364, 1213
HaeIII GGCC 1 cut(s) 415
HapII CCGG 1 cut(s) 600
HhaI GCGC 2 cut(s) 363, 1212
Hin6I GCGC 2 cut(s) 361, 1210
HinP1I GCGC 2 cut(s) 361, 1210
HindIII AAGCTT 2 cut(s) 304, 1088
HpaII CCGG 1 cut(s) 600
HphI GGTGA 2 cut(s) 614, 1423
Hpy166II GTNNAC 4 cut(s) 554, 1157, 1172, 1222
Hpy188I TCNGA 8 cut(s) 987, 1029, 1081, 1180, 1228, 1473, 1628, 1793
Hpy188III TCNNGA 5 cut(s) 722, 1039, 1200, 1256, 1607
Hpy8I GTNNAC 4 cut(s) 554, 1157, 1172, 1222
HpyAV CCTTC 2 cut(s) 937, 1613
HpyCH4III ACNGT 3 cut(s) 210, 551, 1756
HpyCH4IV ACGT 2 cut(s) 610, 1519
HpyCH4V TGCA 8 cut(s) 119, 496, 554, 617, 764, 1172, 1364, 1430
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 369, 932
HpyF3I CTNAG 7 cut(s) 123, 128, 1145, 1206, 1225, 1235, 1632
HpySE526I ACGT 2 cut(s) 610, 1519
HspAI GCGC 2 cut(s) 361, 1210
Ksp22I TGATCA 1 cut(s) 1623
LmnI GCTCC 3 cut(s) 137, 712, 1696
Lsp1109I GCAGC 1 cut(s) 45
LweI GCATC 3 cut(s) 128, 505, 1096
MaeI CTAG 5 cut(s) 332, 807, 834, 1200, 1607
MaeII ACGT 2 cut(s) 610, 1519
MaeIII GTNAC 4 cut(s) 586, 1249, 1304, 1533
MboII GAAGA 6 cut(s) 333, 338, 665, 1543, 1753, 1783
MfeI CAATTG 1 cut(s) 180
MflI RGATCY 2 cut(s) 1677, 1684
MhlI GDGCHC 2 cut(s) 556, 1174
MlsI TGGCCA 1 cut(s) 415
MluNI TGGCCA 1 cut(s) 415
MlyI GAGTC 2 cut(s) 1240, 1391
Mox20I TGGCCA 1 cut(s) 415
Mph1103I ATGCAT 2 cut(s) 121, 1366
MroXI GAANNNNTTC 1 cut(s) 822
MscI TGGCCA 1 cut(s) 415
MseI TTAA 6 cut(s) 251, 543, 977, 1044, 1391, 1760
MslI CAYNNNNRTG 2 cut(s) 858, 1355
Msp20I TGGCCA 1 cut(s) 415
MspA1I CMGCKG 1 cut(s) 1144
MspI CCGG 1 cut(s) 600
MspR9I CCNGG 4 cut(s) 156, 525, 1571, 1682
MunI CAATTG 1 cut(s) 180
Mva1269I GAATGC 1 cut(s) 1366
MvaI CCWGG 4 cut(s) 156, 525, 1571, 1682
MwoI GCNNNNNNNGC 3 cut(s) 104, 369, 932
NdeI CATATG 1 cut(s) 1722
NlaIV GGNNCC 4 cut(s) 378, 521, 598, 1679
NsiI ATGCAT 2 cut(s) 121, 1366
NspI RCATGY 2 cut(s) 98, 1541
PagI TCATGA 1 cut(s) 721
PaqCI CACCTGC 1 cut(s) 940
PcsI WCGNNNNNNNCGW 2 cut(s) 555, 895
PctI GAATGC 1 cut(s) 1366
PdmI GAANNNNTTC 1 cut(s) 822
PfeI GAWTC 8 cut(s) 451, 727, 886, 982, 1118, 1181, 1638, 1715
PflMI CCANNNNNTGG 1 cut(s) 716
PfoI TCCNGGA 1 cut(s) 1680
PinAI ACCGGT 1 cut(s) 599
PkrI GCNGC 1 cut(s) 60
PleI GAGTC 2 cut(s) 1239, 1391
PpsI GAGTC 2 cut(s) 1239, 1391
Ppu21I YACGTR 1 cut(s) 1520
PsiI TTATAA 3 cut(s) 396, 939, 1563
Psp6I CCWGG 4 cut(s) 154, 523, 1569, 1680
PspFI CCCAGC 1 cut(s) 1109
PspGI CCWGG 4 cut(s) 154, 523, 1569, 1680
PspN4I GGNNCC 4 cut(s) 378, 521, 598, 1679
PstI CTGCAG 1 cut(s) 619
PsuI RGATCY 2 cut(s) 1677, 1684
PvuII CAGCTG 1 cut(s) 1144
RsaI GTAC 6 cut(s) 160, 260, 535, 563, 911, 1522
RsaNI GTAC 6 cut(s) 159, 259, 534, 562, 910, 1521
RseI CAYNNNNRTG 2 cut(s) 858, 1355
SaqAI TTAA 6 cut(s) 251, 543, 977, 1044, 1391, 1760
SatI GCNGC 1 cut(s) 59
SchI GAGTC 2 cut(s) 1240, 1391
ScrFI CCNGG 4 cut(s) 156, 525, 1571, 1682
SduI GDGCHC 2 cut(s) 556, 1174
SfaNI GCATC 3 cut(s) 128, 505, 1096
SfcI CTRYAG 2 cut(s) 615, 777
SmiI ATTTAAAT 1 cut(s) 252
SmiMI CAYNNNNRTG 2 cut(s) 858, 1355
SmlI CTYRAG 1 cut(s) 1254
SmoI CTYRAG 1 cut(s) 1254
SsiI CCGC 1 cut(s) 1595
SspMI CTAG 5 cut(s) 332, 807, 834, 1200, 1607
StyD4I CCNGG 4 cut(s) 154, 523, 1569, 1680
StyI CCWWGG 1 cut(s) 681
SwaI ATTTAAAT 1 cut(s) 252
TaaI ACNGT 3 cut(s) 210, 551, 1756
TaiI ACGT 2 cut(s) 613, 1522
TaqI TCGA 2 cut(s) 841, 1018
TatI WGTACW 2 cut(s) 258, 561
TfiI GAWTC 8 cut(s) 451, 727, 886, 982, 1118, 1181, 1638, 1715
Tru1I TTAA 6 cut(s) 251, 543, 977, 1044, 1391, 1760
Tru9I TTAA 6 cut(s) 251, 543, 977, 1044, 1391, 1760
TscAI CASTG 1 cut(s) 347
TseI GCWGC 1 cut(s) 58
TspDTI ATGAA 6 cut(s) 815, 870, 995, 1373, 1430, 1630
TspGWI ACGGA 1 cut(s) 138
TspRI CASTG 1 cut(s) 347
Van91I CCANNNNNTGG 1 cut(s) 716
VneI GTGCAC 2 cut(s) 552, 1170
XapI RAATTY 3 cut(s) 247, 732, 1697
XbaI TCTAGA 2 cut(s) 1199, 1606
XceI RCATGY 2 cut(s) 98, 1541
XcmI CCANNNNNNNNNTGG 1 cut(s) 1701
XmiI GTMKAC 2 cut(s) 1156, 1221
XmnI GAANNNNTTC 1 cut(s) 822
XspI CTAG 5 cut(s) 332, 807, 834, 1200, 1607
Zsp2I ATGCAT 2 cut(s) 121, 1366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.