Rh2BG320800

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0010935
Physical Location & Seq
Forward (+)
38398484 .. 38403702
5219 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG320800.1

Sequence Viewer

Length: 1788 bp
ATGGGAGGAGGTTCAGCTTTTAAATCGCAGTCATGGCAGATGATACTGGTGGCTCTGCTATTGATGGTTGGGTCCTTCTACGCTGGAACTCTCTTTCGCCAAAGTCCTCCCAACTACGTCTCTCAGCTTAGCTCCTACAATTATTCATTTTCTGGTACTTCCACGTTTGCAAATAAAGTTGAATTAACTTATAGAAGAACACCACTTGTGATACCAGAGACGGGTGTGGATGTTTGTCCGTTGACATTCAATGAATATATCCCTTGCCATAATGTTTCATACATACAAACTTTGCTCCCAAATTTGGCCACTAGTAGAAGAGAAGAACTAGAGAGACACTGTCCGCCACTACAGAACCGCTTGTTTTGCTTGGTTCCACCACCCAAAGATTATAAGATACCAATAAGATGGCCAACCAGCAGGGATTATGTGTGGCGAAGCAATGTGAATCATACACATCTTGCTGAAGTCAAAGGAGGACAGAATTGGGTGCATGAGAAGGATCAACTCTGGTGGTTCCCTGGTGGTGGTACCCATTTTAAACATGGTGCACCTGAGTACATTCAGAGATTGGGAAATATGATAACCAATGATACAGGTGATCTACATACTGCAGGGGTCGTTCAAGTTTTGGATGTTGGTTGTGGGGTTTCCAGCTTTTCTGCTTTCCTTCTTCCCTTGGGTATACAGACCATGTCCTTTGCTCCCAAGGATGGCCATGAGAATCAAATTCAATTTTCGCTGGAGCGAGGCATTGGTGCAATGATCTCTGCAATAGCAACAAAGCAATTGCCATATCCTACTAGCTCTTTTGAGATGGTTCATTGTTCTAGGTGTCGTGTTGATTGGCATGAAAATGATGGTATTCTTCTAAAAGAAGTGAATCGCCTTCTGCGTTCTAATGGGTACTTTGTCTATTCAGCTCCACCTGCTTATAGAAAGGATAAAGACTATCCAATAATTTGGGAGAAGTTAGTTAATCTGACTTCAGCAATGTGTTGGAAACTCATTGCTAAAAGAGTTCAGACTGCTATTTGGATGAAGCAAGAAGATCCGTCATGCCTTCAGCGCATTTCTGAACAGAAACTTATAGATATATGTGATGCTGCTGATAATTTGAAGTCATCCTGGAATACAAAACTGGGAAATTGTATACAAGTAAGGGGCATGCAAACAGATGCTCCAAAACTCCCTTCTAGACCAGAGCGTCTTTCAATATACTCGGAGAGTCTTGAAAGAATTGGTATTAGTCAAGAGGAATTTACTTCAGAGACCGTCTTTTGGCAAGATCAAGTTCAACATTACTGGCGGCTGTTGAATGTGAACAAAACAGAAATCCGAAATGTCATGGACATGAATGCTTTTTGTGGTGGATTTGCTGTGGCCTTAGATTCATTACCCATGTGGGTGATGAGTATAGTTCCTGCGACCATGAAGAATACGTTGTCTGCCATTTATGACCGTGGTCTGATTGGTGCTTTCCATGATTGGTGTGAACCATTTTCTACTTATCCACGCACATATGATCTATTGCATGCCAACCACCTCTTTTCTCATTATAAGGAATGTGGAGAAGGCTGTTTGTTGGAGGATATCCTTTTGGAAATGGACAGGATGATAAGACCGCAGGGATTTATTATCATTAGAGATGAGGAAGCTATTACATCAACGATCCAGGATCTTGCTCCAAAATTTTTGTGGGAGGTTGAATTGCATTTGCTGGACAATATAAAGAAGAACAAAGAAACCGTGTTAATTTGTAGGAAGAAGTTTTGGGCAATTGTCTGA

Protein Analysis

595

Amino Acids

68.09

Weight (kDa)

6.79

Isoelectric Point (pI)

44.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 85 - 589 1.7e-201 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11 PF08241 211 - 305 4e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010935)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10200 AT3G10200 AT5G04060
fragaria_vesca FvH4_6g25080 FvH4_6g25080
malus_domestica MD14G1083600.v1.1
prunus_persica Prupe.7G047000_v2.0.a1
pyrus_communis pycom14g07260
rosa_chinensis RchiOBHm_Chr2g0124841
rosa_laevigata RLG00000018828
rosa_multiflora Rmu_co8443503.1_g000001 Rmu_sc0005075.1_g000002
rosa_roxburghii Rroxscaffold_2G00119610 Rroxscaffold_4G00311160
rosa_rugosa Rorug02G0253100
rosa_samantha Rh2AG312600 Rh2BG320800 Rh2CG300900 Rh2DG338100
rosa_wichuraiana Rw2G025250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 393, 1560
AarI CACCTGC 1 cut(s) 937
AasI GACNNNNNNGTC 1 cut(s) 1206
Acc36I ACCTGC 1 cut(s) 937
Acc65I GGTACC 1 cut(s) 530
AccB1I GGYRCC 1 cut(s) 530
AccI GTMKAC 2 cut(s) 685, 1153
AciI CCGC 4 cut(s) 344, 358, 1309, 1625
AclWI GGATC 4 cut(s) 510, 1046, 1666, 1686
AcoI YGGCCR 3 cut(s) 306, 410, 715
AcsI RAATTY 4 cut(s) 301, 729, 1259, 1691
AcuI CTGAAG 4 cut(s) 486, 972, 1049, 1251
AfaI GTAC 4 cut(s) 157, 532, 560, 908
AfiI CCNNNNNNNGG 5 cut(s) 221, 304, 527, 713, 1281
AhlI ACTAGT 1 cut(s) 311
AjnI CCWGG 3 cut(s) 520, 1127, 1674
AjuI GAANNNNNNNTTGG 2 cut(s) 1177, 1209
AluBI AGCT 7 cut(s) 17, 127, 132, 657, 807, 923, 1658
AluI AGCT 7 cut(s) 17, 127, 132, 657, 807, 923, 1658
Alw21I GWGCWC 1 cut(s) 553
Alw26I GTCTC 4 cut(s) 124, 212, 328, 1265
Alw44I GTGCAC 1 cut(s) 549
AlwI GGATC 4 cut(s) 510, 1046, 1666, 1686
AoxI GGCC 4 cut(s) 306, 410, 715, 1383
ApaLI GTGCAC 1 cut(s) 549
ApeKI GCWGC 1 cut(s) 1106
ApoI RAATTY 4 cut(s) 301, 729, 1259, 1691
Asp718I GGTACC 1 cut(s) 530
AspLEI GCGC 1 cut(s) 1071
AspS9I GGNCC 1 cut(s) 72
AsuHPI GGTGA 2 cut(s) 611, 1420
AvaII GGWCC 1 cut(s) 72
BaeGI GKGCMC 1 cut(s) 553
BalI TGGCCA 3 cut(s) 308, 412, 717
BanI GGYRCC 1 cut(s) 530
Bbv12I GWGCWC 1 cut(s) 553
BbvI GCAGC 1 cut(s) 1093
BccI CCATC 5 cut(s) 58, 402, 707, 811, 854
BciT130I CCWGG 3 cut(s) 522, 1129, 1676
BcoDI GTCTC 4 cut(s) 124, 212, 328, 1265
BcuI ACTAGT 1 cut(s) 311
BfaI CTAG 5 cut(s) 312, 329, 804, 831, 1197
BfmI CTRYAG 2 cut(s) 350, 612
BfuAI ACCTGC 1 cut(s) 937
BisI GCNGC 2 cut(s) 1107, 1310
BlpI GCTNAGC 1 cut(s) 128
BlsI GCNGC 2 cut(s) 1108, 1311
Bme1390I CCNGG 3 cut(s) 522, 1129, 1676
Bme18I GGWCC 1 cut(s) 72
BmgT120I GGNCC 1 cut(s) 72
BmiI GGNNCC 4 cut(s) 73, 375, 518, 532
BmrFI CCNGG 3 cut(s) 522, 1129, 1676
BmrI ACTGGG 1 cut(s) 1151
BmsI GCATC 2 cut(s) 1093, 1168
BmuI ACTGGG 1 cut(s) 1151
BoxI GACNNNNGTC 1 cut(s) 1464
BpmI CTGGAG 1 cut(s) 764
Bpu1102I GCTNAGC 1 cut(s) 128
BsaI GGTCTC 1 cut(s) 1265
BsaJI CCNNGG 4 cut(s) 520, 678, 708, 1462
BsaXI ACNNNNNCTCC 2 cut(s) 1165, 1195
Bsc4I CCNNNNNNNGG 5 cut(s) 221, 304, 527, 713, 1281
Bse1I ACTGG 3 cut(s) 51, 1146, 1310
Bse3DI GCAATG 4 cut(s) 448, 768, 999, 1008
BseBI CCWGG 3 cut(s) 522, 1129, 1676
BseDI CCNNGG 4 cut(s) 520, 678, 708, 1462
BseGI GGATG 6 cut(s) 235, 640, 718, 1044, 1124, 1620
BseLI CCNNNNNNNGG 5 cut(s) 221, 304, 527, 713, 1281
BseMI GCAATG 4 cut(s) 448, 768, 999, 1008
BseMII CTCAG 2 cut(s) 137, 546
BseNI ACTGG 3 cut(s) 51, 1146, 1310
BseRI GAGGAG 1 cut(s) 21
BseSI GKGCMC 1 cut(s) 553
BseXI GCAGC 1 cut(s) 1093
BshFI GGCC 4 cut(s) 308, 412, 717, 1385
BshNI GGYRCC 1 cut(s) 530
BsiHKAI GWGCWC 1 cut(s) 553
BslI CCNNNNNNNGG 5 cut(s) 221, 304, 527, 713, 1281
BsmAI GTCTC 4 cut(s) 124, 212, 328, 1265
BsmBI CGTCTC 2 cut(s) 124, 212
BsmI GAATGC 1 cut(s) 1363
BsnI GGCC 4 cut(s) 308, 412, 717, 1385
Bso31I GGTCTC 1 cut(s) 1265
Bsp1286I GDGCHC 1 cut(s) 553
Bsp143I GATC 8 cut(s) 502, 601, 765, 1051, 1288, 1525, 1671, 1678
Bsp1720I GCTNAGC 1 cut(s) 128
BspACI CCGC 4 cut(s) 344, 358, 1309, 1625
BspANI GGCC 4 cut(s) 308, 412, 717, 1385
BspCNI CTCAG 2 cut(s) 136, 547
BspLI GGNNCC 4 cut(s) 73, 375, 518, 532
BspMAI CTGCAG 1 cut(s) 616
BspMI ACCTGC 1 cut(s) 937
BspPI GGATC 4 cut(s) 510, 1046, 1666, 1686
BspT107I GGYRCC 1 cut(s) 530
BspTNI GGTCTC 1 cut(s) 1265
BsrDI GCAATG 4 cut(s) 448, 768, 999, 1008
BsrI ACTGG 3 cut(s) 51, 1146, 1310
BssECI CCNNGG 4 cut(s) 520, 678, 708, 1462
BssMI GATC 8 cut(s) 502, 601, 765, 1051, 1288, 1525, 1671, 1678
BssNAI GTATAC 2 cut(s) 686, 1154
BssT1I CCWWGG 2 cut(s) 678, 708
Bst1107I GTATAC 2 cut(s) 686, 1154
Bst2UI CCWGG 3 cut(s) 522, 1129, 1676
Bst4CI ACNGT 4 cut(s) 341, 1276, 1463, 1750
Bst6I CTCTTC 1 cut(s) 313
BstC8I GCNNGC 2 cut(s) 1169, 1536
BstDEI CTNAG 4 cut(s) 123, 128, 555, 1387
BstDSI CCRYGG 1 cut(s) 1462
BstF5I GGATG 6 cut(s) 235, 640, 718, 1044, 1124, 1620
BstHHI GCGC 1 cut(s) 1071
BstKTI GATC 8 cut(s) 505, 604, 768, 1054, 1291, 1528, 1674, 1681
BstMAI GTCTC 4 cut(s) 124, 212, 328, 1265
BstMBI GATC 8 cut(s) 502, 601, 765, 1051, 1288, 1525, 1671, 1678
BstMWI GCNNNNNNNGC 4 cut(s) 34, 366, 929, 1068
BstNI CCWGG 3 cut(s) 522, 1129, 1676
BstNSI RCATGY 2 cut(s) 1171, 1538
BstPAI GACNNNNGTC 1 cut(s) 1464
BstSCI CCNGG 3 cut(s) 520, 1127, 1674
BstSFI CTRYAG 2 cut(s) 350, 612
BstSLI GKGCMC 1 cut(s) 553
BstV1I GCAGC 1 cut(s) 1093
BstX2I RGATCY 2 cut(s) 1051, 1678
BstXI CCANNNNNNTGG 2 cut(s) 408, 963
BstYI RGATCY 2 cut(s) 1051, 1678
BstZ17I GTATAC 2 cut(s) 686, 1154
BsuRI GGCC 4 cut(s) 308, 412, 717, 1385
BtgI CCRYGG 1 cut(s) 1462
BtsCI GGATG 6 cut(s) 235, 640, 718, 1044, 1124, 1620
BtsIMutI CAGTG 1 cut(s) 337
BveI ACCTGC 1 cut(s) 937
Cac8I GCNNGC 2 cut(s) 1169, 1536
CfoI GCGC 1 cut(s) 1071
Cfr13I GGNCC 1 cut(s) 72
CseI GACGC 1 cut(s) 1196
Csp6I GTAC 4 cut(s) 156, 531, 559, 907
CspCI CAANNNNNGTGG 2 cut(s) 494, 529
CviQI GTAC 4 cut(s) 156, 531, 559, 907
DdeI CTNAG 4 cut(s) 123, 128, 555, 1387
DpnI GATC 8 cut(s) 504, 603, 767, 1053, 1290, 1527, 1673, 1680
DpnII GATC 8 cut(s) 502, 601, 765, 1051, 1288, 1525, 1671, 1678
DraI TTTAAA 2 cut(s) 22, 541
DrdI GACNNNNNNGTC 1 cut(s) 1206
DseDI GACNNNNNNGTC 1 cut(s) 1206
EaeI YGGCCR 3 cut(s) 306, 410, 715
Eam1104I CTCTTC 1 cut(s) 313
EarI CTCTTC 1 cut(s) 313
EciI GGCGGA 1 cut(s) 333
Eco130I CCWWGG 2 cut(s) 678, 708
Eco31I GGTCTC 1 cut(s) 1265
Eco32I GATATC 1 cut(s) 1594
Eco47I GGWCC 1 cut(s) 72
Eco57I CTGAAG 4 cut(s) 486, 972, 1049, 1251
EcoO109I RGGNCCY 1 cut(s) 72
EcoRII CCWGG 3 cut(s) 520, 1127, 1674
EcoRV GATATC 1 cut(s) 1594
EcoT14I CCWWGG 2 cut(s) 678, 708
ErhI CCWWGG 2 cut(s) 678, 708
Esp3I CGTCTC 2 cut(s) 124, 212
FauNDI CATATG 1 cut(s) 1522
FblI GTMKAC 2 cut(s) 685, 1153
Fnu4HI GCNGC 2 cut(s) 1107, 1310
FokI GGATG 6 cut(s) 242, 647, 725, 1051, 1111, 1627
Fsp4HI GCNGC 2 cut(s) 1107, 1310
FspBI CTAG 5 cut(s) 312, 329, 804, 831, 1197
GlaI GCGC 1 cut(s) 1070
GluI GCNGC 2 cut(s) 1107, 1310
GsuI CTGGAG 1 cut(s) 764
HaeIII GGCC 4 cut(s) 308, 412, 717, 1385
HgaI GACGC 1 cut(s) 1196
HhaI GCGC 1 cut(s) 1071
Hin6I GCGC 1 cut(s) 1069
HinP1I GCGC 1 cut(s) 1069
HincII GTYRAC 1 cut(s) 243
HindII GTYRAC 1 cut(s) 243
HinfI GANTC 5 cut(s) 448, 724, 883, 1228, 1391
HphI GGTGA 2 cut(s) 611, 1420
Hpy166II GTNNAC 6 cut(s) 243, 551, 686, 1154, 1324, 1496
Hpy188I TCNGA 9 cut(s) 567, 984, 1026, 1078, 1225, 1270, 1340, 1470, 1787
Hpy188III TCNNGA 3 cut(s) 1197, 1232, 1253
Hpy8I GTNNAC 6 cut(s) 243, 551, 686, 1154, 1324, 1496
HpyAV CCTTC 7 cut(s) 85, 493, 680, 899, 1073, 1203, 1568
HpyCH4III ACNGT 4 cut(s) 341, 1276, 1463, 1750
HpyCH4IV ACGT 3 cut(s) 117, 164, 1442
HpyCH4V TGCA 9 cut(s) 170, 493, 551, 614, 761, 773, 1171, 1534, 1714
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 366, 929, 1068
HpyF3I CTNAG 4 cut(s) 123, 128, 555, 1387
HpySE526I ACGT 3 cut(s) 117, 164, 1442
HspAI GCGC 1 cut(s) 1069
KpnI GGTACC 1 cut(s) 534
Kzo9I GATC 8 cut(s) 502, 601, 765, 1051, 1288, 1525, 1671, 1678
LmnI GCTCC 7 cut(s) 137, 300, 709, 745, 928, 1186, 1690
Lsp1109I GCAGC 1 cut(s) 1093
LweI GCATC 2 cut(s) 1093, 1168
MaeI CTAG 5 cut(s) 312, 329, 804, 831, 1197
MaeII ACGT 3 cut(s) 117, 164, 1442
MalI GATC 8 cut(s) 504, 603, 767, 1053, 1290, 1527, 1673, 1680
MboI GATC 8 cut(s) 502, 601, 765, 1051, 1288, 1525, 1671, 1678
MboII GAAGA 9 cut(s) 207, 330, 335, 665, 860, 1061, 1447, 1747, 1777
MfeI CAATTG 2 cut(s) 788, 1779
MflI RGATCY 2 cut(s) 1051, 1678
MhlI GDGCHC 1 cut(s) 553
MlsI TGGCCA 3 cut(s) 308, 412, 717
MluNI TGGCCA 3 cut(s) 308, 412, 717
MlyI GAGTC 1 cut(s) 1237
MmeI TCCRAC 2 cut(s) 980, 1566
MnlI CCTC 8 cut(s) 117, 470, 743, 1249, 1556, 1582, 1645, 1696
Mox20I TGGCCA 3 cut(s) 308, 412, 717
MscI TGGCCA 3 cut(s) 308, 412, 717
MseI TTAA 5 cut(s) 21, 185, 540, 978, 1754
MslI CAYNNNNRTG 3 cut(s) 855, 1352, 1406
Msp20I TGGCCA 3 cut(s) 308, 412, 717
MspR9I CCNGG 3 cut(s) 522, 1129, 1676
MunI CAATTG 2 cut(s) 788, 1779
Mva1269I GAATGC 1 cut(s) 1363
MvaI CCWGG 3 cut(s) 522, 1129, 1676
MwoI GCNNNNNNNGC 4 cut(s) 34, 366, 929, 1068
NdeI CATATG 1 cut(s) 1522
NdeII GATC 8 cut(s) 502, 601, 765, 1051, 1288, 1525, 1671, 1678
NlaIV GGNNCC 4 cut(s) 73, 375, 518, 532
NspI RCATGY 2 cut(s) 1171, 1538
PaeI GCATGC 2 cut(s) 1171, 1538
PaqCI CACCTGC 1 cut(s) 937
PcsI WCGNNNNNNNCGW 1 cut(s) 892
PctI GAATGC 1 cut(s) 1363
PfeI GAWTC 4 cut(s) 448, 724, 883, 1391
PflFI GACNNNGTC 2 cut(s) 339, 694
PfoI TCCNGGA 2 cut(s) 1127, 1674
PkrI GCNGC 2 cut(s) 1108, 1311
PleI GAGTC 1 cut(s) 1236
PpsI GAGTC 1 cut(s) 1236
PpuMI RGGWCCY 1 cut(s) 72
PshAI GACNNNNGTC 1 cut(s) 1464
PsiI TTATAA 2 cut(s) 393, 1560
Psp5II RGGWCCY 1 cut(s) 72
Psp6I CCWGG 3 cut(s) 520, 1127, 1674
PspGI CCWGG 3 cut(s) 520, 1127, 1674
PspN4I GGNNCC 4 cut(s) 73, 375, 518, 532
PspPI GGNCC 1 cut(s) 72
PspPPI RGGWCCY 1 cut(s) 72
PstI CTGCAG 1 cut(s) 616
PsuI RGATCY 2 cut(s) 1051, 1678
PsyI GACNNNGTC 2 cut(s) 339, 694
RsaI GTAC 4 cut(s) 157, 532, 560, 908
RsaNI GTAC 4 cut(s) 156, 531, 559, 907
RseI CAYNNNNRTG 3 cut(s) 855, 1352, 1406
SaqAI TTAA 5 cut(s) 21, 185, 540, 978, 1754
SatI GCNGC 2 cut(s) 1107, 1310
Sau3AI GATC 8 cut(s) 502, 601, 765, 1051, 1288, 1525, 1671, 1678
Sau96I GGNCC 1 cut(s) 72
SchI GAGTC 1 cut(s) 1237
ScrFI CCNGG 3 cut(s) 522, 1129, 1676
SduI GDGCHC 1 cut(s) 553
SfaNI GCATC 2 cut(s) 1093, 1168
SfcI CTRYAG 2 cut(s) 350, 612
SinI GGWCC 1 cut(s) 72
SmiMI CAYNNNNRTG 3 cut(s) 855, 1352, 1406
SpeI ACTAGT 1 cut(s) 311
SphI GCATGC 2 cut(s) 1171, 1538
SsiI CCGC 4 cut(s) 344, 358, 1309, 1625
SspMI CTAG 5 cut(s) 312, 329, 804, 831, 1197
StyD4I CCNGG 3 cut(s) 520, 1127, 1674
StyI CCWWGG 2 cut(s) 678, 708
TaaI ACNGT 4 cut(s) 341, 1276, 1463, 1750
TaiI ACGT 3 cut(s) 120, 167, 1445
TatI WGTACW 1 cut(s) 558
TauI GCSGC 1 cut(s) 1312
TfiI GAWTC 4 cut(s) 448, 724, 883, 1391
Tru1I TTAA 5 cut(s) 21, 185, 540, 978, 1754
Tru9I TTAA 5 cut(s) 21, 185, 540, 978, 1754
TscAI CASTG 1 cut(s) 344
TseI GCWGC 1 cut(s) 1106
TspDTI ATGAA 9 cut(s) 135, 267, 267, 812, 867, 1055, 1370, 1383, 1448
TspGWI ACGGA 2 cut(s) 228, 1044
TspRI CASTG 1 cut(s) 344
Tth111I GACNNNGTC 2 cut(s) 339, 694
VneI GTGCAC 1 cut(s) 549
VpaK11BI GGWCC 1 cut(s) 72
XapI RAATTY 4 cut(s) 301, 729, 1259, 1691
XbaI TCTAGA 1 cut(s) 1196
XceI RCATGY 2 cut(s) 1171, 1538
XcmI CCANNNNNNNNNTGG 2 cut(s) 542, 1695
XmiI GTMKAC 2 cut(s) 685, 1153
XspI CTAG 5 cut(s) 312, 329, 804, 831, 1197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.