AT5G04060

methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010935
Physical Location & Seq
Forward (+)
1099029 .. 1102058
3030 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G04060.1

Sequence Viewer

Length: 1803 bp
ATGGGAGGAGGTTATGTGTTGTTTGGTTCGGCTAGATCGGGGCAGATGATAATGGTGGCTCTGGTCCTAATGGTTGGATCATTTTACGCAGGCTCTATCTTCGGAAACAATTCACCTATTTACATTTCCCAACCTTCTTCTTCCAATTCTTCTTCTTCTTCTCCATCACAATCTGGTCCATCAAATTTTGCTAACAAAATAGAGCTCACTTACCGGAGAACTTCTGTTTCGATCCCTGAGAGTGGGGTGAATGTTTGTCCATTGAAGTTCAATGAGTATATACCTTGTCACAATGTCACGTATGTGCAACAGCTTCTCCCAAGCTTGAATCTTTCTAGAAGAGAAGAGCTTGAGAGACACTGTCCGCCACTCGAACAGCGTCTGTTTTGTTTGGTTCCTCCGCCTAAAGATTATAAGATACCAATACGCTGGCCGACTAGCAGAGATTATGTATGGAGAAGCAATGTGAATCATACGCATCTTGCTGAAGTGAAAGGCGGCCAAAATTGGGTGCATGAACAAGGTCAGTTATGGTGGTTCCCTGGTGGTGGTACTCATTTCAAACATGGAGCTCCGGAATACATCCAGAGGTTAGGAAACATGACAACTAATGAAACAGGTGACCTTCTTTCAGCTGGGGTTGAACAAGTTCTTGATGTTGGATGTGGAGTTGCTAGCTTTGCAGCTTATCTTCTTCCTTTAGGTATAAAGACAATGTCTTTTGCCCCTAAAGATGGTCATGAAAACCAAATCCAGTTTGCATTGGAGAGAGGAATCCGCGCAATGATCTCTGCCATAGCCACCAAACAAATGCCATATCCCGCGGCCTCGTTTGATATGGTTCACTGTTCAAGATGTCGTGTTGATTGGCATGAAAATGATGGTGTCTTGATGAAAGAGGTTAATCGTCTTCTCCGACCCAATGGATACTTTGTTTACTCGGCACCACCTGCGTACAGAAAGGATAAAGATTTCCCTGTGATTTGGGATAAGTTGGTCAATCTAACTAGCGCAATGTGCTGGAAGCTCATTTCCCGAAAGGTACAGACTGCAATATGGGTTAAAGAAGATGACGAGGCTTGCCTTAGGAAGAATGCAGAGCTAGAGCTTATAACTATATGTGGTGTAGAAGATGTTTCGAAAGCTTCATGGAAAGTTCCTCTTAGAGACTGTGTGGACATTAGTGAAAATAGACAACAGAAACCCTCTTCTTTAACTGACCGTCTATCTTCATATCCAACAAGTCTAAGAGAAAAAGGCATCAGCGAAGACGAATTTACATTGGACACAAACTTCTGGAGAGAACAAGTGAATCAGTACTGGGAGTTGATGAATGTTAATAAGACTGAAGTGCGAAATGTGATGGACACAAATGCATTCATTGGTGGATTTGCCGCAGCCATGAACTCATATCCCCTTTGGGTCATGAACGTTGTACCTGCTACAATGAACGATACCTTATCCGGAATTTACCAGAGGGGCTTAACCGGTGCTTATCATGATTGGTGTGAGCCATTCTCAACATATCCCCGTACTTACGATCTGCTACACGCGGACCATCTCTTCACTCACTATAAAATCTACGGTGAAGGTTGTTTACTAGAGGACATCATGCTTGAGATGGACCGCATTATACGCCCTCAGGGATTTATCATTATCAGAGACGAGGAATCAATCGTCTCAAGAGTCCGAGACTTGGCTCCTAAGTTCCTTTGGGAAGTTGAAGCACATGAGCTTCAAGACAAATACAAGAAGACAGAAACTGTTCTATTTTGCAGAAAGAAATTTTGGGCAATCCTTTGA

Protein Analysis

600

Amino Acids

68.36

Weight (kDa)

6.45

Isoelectric Point (pI)

41.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 92 - 594 6.9e-213 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_25 PF13649 217 - 309 6.4e-08 Methyltransferase domain
Methyltransf_11 PF08241 218 - 312 6.6e-10 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010935)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10200 AT3G10200 AT5G04060
fragaria_vesca FvH4_6g25080 FvH4_6g25080
malus_domestica MD14G1083600.v1.1
prunus_persica Prupe.7G047000_v2.0.a1
pyrus_communis pycom14g07260
rosa_chinensis RchiOBHm_Chr2g0124841
rosa_laevigata RLG00000018828
rosa_multiflora Rmu_co8443503.1_g000001 Rmu_sc0005075.1_g000002
rosa_roxburghii Rroxscaffold_2G00119610 Rroxscaffold_4G00311160
rosa_rugosa Rorug02G0253100
rosa_samantha Rh2AG312600 Rh2BG320800 Rh2CG300900 Rh2DG338100
rosa_wichuraiana Rw2G025250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 414, 1112
AarI CACCTGC 1 cut(s) 958
Acc36I ACCTGC 2 cut(s) 958, 1447
AccB1I GGYRCC 1 cut(s) 943
AccII CGCG 3 cut(s) 780, 824, 1553
AccIII TCCGGA 2 cut(s) 574, 1463
AciI CCGC 9 cut(s) 365, 401, 498, 778, 822, 824, 1395, 1553, 1627
AclI AACGTT 1 cut(s) 1431
AclWI GGATC 2 cut(s) 85, 226
AcoI YGGCCR 2 cut(s) 431, 499
AcsI RAATTY 4 cut(s) 184, 1274, 1467, 1784
AcuI CTGAAG 2 cut(s) 507, 1368
AfaI GTAC 6 cut(s) 553, 956, 1044, 1319, 1437, 1534
AfiI CCNNNNNNNGG 5 cut(s) 242, 508, 548, 734, 1696
AgeI ACCGGT 1 cut(s) 1487
AgsI TTSAA 8 cut(s) 265, 271, 328, 562, 644, 852, 1724, 1739
AjnI CCWGG 1 cut(s) 541
AjuI GAANNNNNNNTTGG 2 cut(s) 1771, 1803
AleI CACNNNNGTG 1 cut(s) 302
Alw21I GWGCWC 2 cut(s) 207, 574
Alw26I GTCTC 5 cut(s) 349, 1161, 1656, 1684, 1686
AlwI GGATC 2 cut(s) 85, 226
AlwNI CAGNNNCTG 2 cut(s) 382, 1763
Aor13HI TCCGGA 2 cut(s) 574, 1463
AoxI GGCC 3 cut(s) 431, 499, 825
ApeKI GCWGC 2 cut(s) 683, 1397
ApoI RAATTY 4 cut(s) 184, 1274, 1467, 1784
AsiGI ACCGGT 1 cut(s) 1487
Asp700I GAANNNNTTC 3 cut(s) 109, 648, 1764
AspLEI GCGC 2 cut(s) 782, 1013
AspS9I GGNCC 4 cut(s) 64, 176, 1555, 1624
AsuHPI GGTGA 4 cut(s) 105, 259, 632, 1598
AsuII TTCGAA 1 cut(s) 1139
AsuNHI GCTAGC 1 cut(s) 674
AvaII GGWCC 4 cut(s) 64, 176, 1555, 1624
AxyI CCTNAGG 2 cut(s) 1085, 1641
BanI GGYRCC 1 cut(s) 943
BanII GRGCYC 2 cut(s) 207, 574
BarI GAAGNNNNNNTAC 2 cut(s) 1581, 1613
BbsI GAAGAC 3 cut(s) 902, 1275, 1760
Bbv12I GWGCWC 2 cut(s) 207, 574
BbvI GCAGC 2 cut(s) 695, 1409
BccI CCATC 7 cut(s) 172, 187, 728, 875, 1357, 1566, 1615
BciT130I CCWGG 1 cut(s) 543
BciVI GTATCC 1 cut(s) 920
BcoDI GTCTC 5 cut(s) 349, 1161, 1656, 1684, 1686
BfaI CTAG 7 cut(s) 33, 336, 438, 675, 1008, 1103, 1601
BfuAI ACCTGC 2 cut(s) 958, 1447
BfuI GTATCC 1 cut(s) 920
BisI GCNGC 5 cut(s) 499, 684, 825, 1395, 1398
BlsI GCNGC 5 cut(s) 500, 685, 826, 1396, 1399
BmcAI AGTACT 1 cut(s) 1319
Bme1390I CCNGG 1 cut(s) 543
Bme18I GGWCC 4 cut(s) 64, 176, 1555, 1624
BmgT120I GGNCC 4 cut(s) 64, 176, 1555, 1624
BmiI GGNNCC 4 cut(s) 396, 539, 945, 1701
BmrFI CCNGG 1 cut(s) 543
BmrI ACTGGG 1 cut(s) 1330
BmsI GCATC 2 cut(s) 487, 1269
BmtI GCTAGC 1 cut(s) 678
BmuI ACTGGG 1 cut(s) 1330
BpiI GAAGAC 3 cut(s) 902, 1275, 1760
BplI GAGNNNNNCTC 2 cut(s) 1502, 1534
BpmI CTGGAG 1 cut(s) 1318
Bpu14I TTCGAA 1 cut(s) 1139
BpuEI CTTGAG 3 cut(s) 371, 1637, 1666
BsaAI YACGTR 1 cut(s) 300
BsaJI CCNNGG 2 cut(s) 541, 822
BsaWI WCCGGW 4 cut(s) 213, 574, 1463, 1487
BsaXI ACNNNNNCTCC 3 cut(s) 30, 300, 330
Bsc4I CCNNNNNNNGG 5 cut(s) 242, 508, 548, 734, 1696
Bse118I RCCGGY 1 cut(s) 1487
Bse1I ACTGG 2 cut(s) 754, 1325
Bse21I CCTNAGG 2 cut(s) 1085, 1641
Bse3DI GCAATG 3 cut(s) 469, 789, 1020
BseAI TCCGGA 2 cut(s) 574, 1463
BseBI CCWGG 1 cut(s) 543
BseDI CCNNGG 2 cut(s) 541, 822
BseGI GGATG 2 cut(s) 582, 668
BseLI CCNNNNNNNGG 5 cut(s) 242, 508, 548, 734, 1696
BseMI GCAATG 3 cut(s) 469, 789, 1020
BseMII CTCAG 2 cut(s) 228, 1655
BseNI ACTGG 2 cut(s) 754, 1325
BseRI GAGGAG 1 cut(s) 21
BseXI GCAGC 2 cut(s) 695, 1409
BseYI CCCAGC 1 cut(s) 635
Bsh1236I CGCG 3 cut(s) 780, 824, 1553
BshFI GGCC 3 cut(s) 433, 501, 827
BshNI GGYRCC 1 cut(s) 943
BshTI ACCGGT 1 cut(s) 1487
BsiHKAI GWGCWC 2 cut(s) 207, 574
BsiSI CCGG 4 cut(s) 214, 575, 1464, 1488
BslI CCNNNNNNNGG 5 cut(s) 242, 508, 548, 734, 1696
BsmAI GTCTC 5 cut(s) 349, 1161, 1656, 1684, 1686
BsmBI CGTCTC 2 cut(s) 1656, 1684
BsmI GAATGC 2 cut(s) 1099, 1376
BsnI GGCC 3 cut(s) 433, 501, 827
Bsp119I TTCGAA 1 cut(s) 1139
Bsp1286I GDGCHC 2 cut(s) 207, 574
Bsp13I TCCGGA 2 cut(s) 574, 1463
Bsp143I GATC 5 cut(s) 35, 77, 231, 786, 1540
BspACI CCGC 9 cut(s) 365, 401, 498, 778, 822, 824, 1395, 1553, 1627
BspANI GGCC 3 cut(s) 433, 501, 827
BspCNI CTCAG 2 cut(s) 229, 1654
BspEI TCCGGA 2 cut(s) 574, 1463
BspFNI CGCG 3 cut(s) 780, 824, 1553
BspHI TCATGA 3 cut(s) 739, 1425, 1498
BspLI GGNNCC 4 cut(s) 396, 539, 945, 1701
BspMI ACCTGC 2 cut(s) 958, 1447
BspOI GCTAGC 1 cut(s) 678
BspPI GGATC 2 cut(s) 85, 226
BspQI GCTCTTC 1 cut(s) 339
BspT104I TTCGAA 1 cut(s) 1139
BspT107I GGYRCC 1 cut(s) 943
BsrDI GCAATG 3 cut(s) 469, 789, 1020
BsrFI RCCGGY 1 cut(s) 1487
BsrI ACTGG 2 cut(s) 754, 1325
BssAI RCCGGY 1 cut(s) 1487
BssECI CCNNGG 2 cut(s) 541, 822
BssMI GATC 5 cut(s) 35, 77, 231, 786, 1540
Bst2UI CCWGG 1 cut(s) 543
Bst4CI ACNGT 6 cut(s) 362, 848, 1172, 1223, 1586, 1765
Bst6I CTCTTC 4 cut(s) 334, 339, 1213, 1568
BstAPI GCANNNNNTGC 1 cut(s) 950
BstBAI YACGTR 1 cut(s) 300
BstBI TTCGAA 1 cut(s) 1139
BstC8I GCNNGC 4 cut(s) 91, 431, 676, 1081
BstDEI CTNAG 6 cut(s) 237, 1085, 1163, 1247, 1641, 1704
BstDSI CCRYGG 1 cut(s) 822
BstEII GGTNACC 1 cut(s) 620
BstF5I GGATG 2 cut(s) 582, 668
BstFNI CGCG 3 cut(s) 780, 824, 1553
BstHHI GCGC 2 cut(s) 782, 1013
BstKTI GATC 5 cut(s) 38, 80, 234, 789, 1543
BstMAI GTCTC 5 cut(s) 349, 1161, 1656, 1684, 1686
BstMBI GATC 5 cut(s) 35, 77, 231, 786, 1540
BstMWI GCNNNNNNNGC 4 cut(s) 680, 950, 1017, 1635
BstNI CCWGG 1 cut(s) 543
BstPI GGTNACC 1 cut(s) 620
BstSCI CCNGG 1 cut(s) 541
BstUI CGCG 3 cut(s) 780, 824, 1553
BstV1I GCAGC 2 cut(s) 695, 1409
BstV2I GAAGAC 3 cut(s) 902, 1275, 1760
BstXI CCANNNNNNTGG 1 cut(s) 429
Bsu36I CCTNAGG 2 cut(s) 1085, 1641
BsuI GTATCC 1 cut(s) 920
BsuRI GGCC 3 cut(s) 433, 501, 827
BtgI CCRYGG 1 cut(s) 822
BtsCI GGATG 2 cut(s) 582, 668
BtsIMutI CAGTG 2 cut(s) 358, 844
BveI ACCTGC 2 cut(s) 958, 1447
Cac8I GCNNGC 4 cut(s) 91, 431, 676, 1081
CaiI CAGNNNCTG 2 cut(s) 382, 1763
CciI TCATGA 3 cut(s) 739, 1425, 1498
CfoI GCGC 2 cut(s) 782, 1013
Cfr10I RCCGGY 1 cut(s) 1487
Cfr13I GGNCC 4 cut(s) 64, 176, 1555, 1624
Cfr42I CCGCGG 1 cut(s) 825
CseI GACGC 1 cut(s) 368
Csp6I GTAC 6 cut(s) 552, 955, 1043, 1318, 1436, 1533
CspAI ACCGGT 1 cut(s) 1487
CviQI GTAC 6 cut(s) 552, 955, 1043, 1318, 1436, 1533
DdeI CTNAG 6 cut(s) 237, 1085, 1163, 1247, 1641, 1704
DpnI GATC 5 cut(s) 37, 79, 233, 788, 1542
DpnII GATC 5 cut(s) 35, 77, 231, 786, 1540
EaeI YGGCCR 2 cut(s) 431, 499
Eam1104I CTCTTC 4 cut(s) 334, 339, 1213, 1568
EarI CTCTTC 4 cut(s) 334, 339, 1213, 1568
EciI GGCGGA 2 cut(s) 354, 390
Ecl136II GAGCTC 2 cut(s) 205, 572
Eco24I GRGCYC 2 cut(s) 207, 574
Eco47I GGWCC 4 cut(s) 64, 176, 1555, 1624
Eco53kI GAGCTC 2 cut(s) 205, 572
Eco57I CTGAAG 2 cut(s) 507, 1368
Eco81I CCTNAGG 2 cut(s) 1085, 1641
Eco91I GGTNACC 1 cut(s) 620
EcoICRI GAGCTC 2 cut(s) 205, 572
EcoO65I GGTNACC 1 cut(s) 620
EcoRII CCWGG 1 cut(s) 541
EcoT22I ATGCAT 1 cut(s) 1378
EcoT38I GRGCYC 2 cut(s) 207, 574
Esp3I CGTCTC 2 cut(s) 1656, 1684
FalI AAGNNNNNCTT 2 cut(s) 1146, 1178
FauI CCCGC 1 cut(s) 829
Fnu4HI GCNGC 5 cut(s) 499, 684, 825, 1395, 1398
FokI GGATG 2 cut(s) 569, 675
FriOI GRGCYC 2 cut(s) 207, 574
Fsp4HI GCNGC 5 cut(s) 499, 684, 825, 1395, 1398
FspBI CTAG 7 cut(s) 33, 336, 438, 675, 1008, 1103, 1601
GlaI GCGC 2 cut(s) 781, 1012
GluI GCNGC 5 cut(s) 499, 684, 825, 1395, 1398
GsaI CCCAGC 1 cut(s) 639
GsuI CTGGAG 1 cut(s) 1318
HaeIII GGCC 3 cut(s) 433, 501, 827
HapII CCGG 4 cut(s) 214, 575, 1464, 1488
HgaI GACGC 1 cut(s) 368
HhaI GCGC 2 cut(s) 782, 1013
Hin6I GCGC 2 cut(s) 780, 1011
HinP1I GCGC 2 cut(s) 780, 1011
HindIII AAGCTT 2 cut(s) 322, 1143
HinfI GANTC 6 cut(s) 328, 469, 774, 1312, 1670, 1686
HpaII CCGG 4 cut(s) 214, 575, 1464, 1488
HphI GGTGA 4 cut(s) 105, 259, 632, 1598
Hpy166II GTNNAC 4 cut(s) 844, 937, 1177, 1598
Hpy188I TCNGA 4 cut(s) 104, 917, 1661, 1691
Hpy8I GTNNAC 4 cut(s) 844, 937, 1177, 1598
HpyAV CCTTC 3 cut(s) 144, 635, 1583
HpyCH4III ACNGT 6 cut(s) 362, 848, 1172, 1223, 1586, 1765
HpyCH4IV ACGT 2 cut(s) 299, 1431
HpyCH4V TGCA 8 cut(s) 307, 514, 683, 761, 1052, 1097, 1376, 1776
HpyF10VI GCNNNNNNNGC 4 cut(s) 680, 950, 1017, 1635
HpyF3I CTNAG 6 cut(s) 237, 1085, 1163, 1247, 1641, 1704
HpySE526I ACGT 2 cut(s) 299, 1431
HspAI GCGC 2 cut(s) 780, 1011
Kpn2I TCCGGA 2 cut(s) 574, 1463
KspI CCGCGG 1 cut(s) 825
Kzo9I GATC 5 cut(s) 35, 77, 231, 786, 1540
LguI GCTCTTC 1 cut(s) 339
LmnI GCTCC 3 cut(s) 569, 577, 1705
Lsp1109I GCAGC 2 cut(s) 695, 1409
LweI GCATC 2 cut(s) 487, 1269
MaeI CTAG 7 cut(s) 33, 336, 438, 675, 1008, 1103, 1601
MaeII ACGT 2 cut(s) 299, 1431
MaeIII GTNAC 3 cut(s) 287, 295, 620
MalI GATC 5 cut(s) 37, 79, 233, 788, 1542
MboI GATC 5 cut(s) 35, 77, 231, 786, 1540
MhlI GDGCHC 2 cut(s) 207, 574
MluCI AATT 7 cut(s) 109, 145, 184, 505, 1274, 1467, 1784
MlyI GAGTC 1 cut(s) 1695
MmeI TCCRAC 4 cut(s) 55, 640, 940, 1262
Mph1103I ATGCAT 1 cut(s) 1378
MroI TCCGGA 2 cut(s) 574, 1463
MroXI GAANNNNTTC 3 cut(s) 109, 648, 1764
MseI TTAA 5 cut(s) 903, 1062, 1214, 1338, 1484
MslI CAYNNNNRTG 2 cut(s) 302, 876
MspA1I CMGCKG 2 cut(s) 635, 824
MspI CCGG 4 cut(s) 214, 575, 1464, 1488
MspR9I CCNGG 1 cut(s) 543
Mva1269I GAATGC 2 cut(s) 1099, 1376
MvaI CCWGG 1 cut(s) 543
MvnI CGCG 3 cut(s) 780, 824, 1553
MwoI GCNNNNNNNGC 4 cut(s) 680, 950, 1017, 1635
NdeII GATC 5 cut(s) 35, 77, 231, 786, 1540
NheI GCTAGC 1 cut(s) 674
NlaIV GGNNCC 4 cut(s) 396, 539, 945, 1701
NmeAIII GCCGAG 1 cut(s) 920
NmuCI GTSAC 3 cut(s) 287, 295, 620
NsiI ATGCAT 1 cut(s) 1378
NspV TTCGAA 1 cut(s) 1139
OliI CACNNNNGTG 1 cut(s) 302
PagI TCATGA 3 cut(s) 739, 1425, 1498
PaqCI CACCTGC 1 cut(s) 958
PciSI GCTCTTC 1 cut(s) 339
PcsI WCGNNNNNNNCGW 1 cut(s) 913
PctI GAATGC 2 cut(s) 1099, 1376
PdmI GAANNNNTTC 3 cut(s) 109, 648, 1764
PfeI GAWTC 5 cut(s) 328, 469, 774, 1312, 1670
PflFI GACNNNGTC 2 cut(s) 360, 715
PinAI ACCGGT 1 cut(s) 1487
PkrI GCNGC 5 cut(s) 500, 685, 826, 1396, 1399
PleI GAGTC 1 cut(s) 1694
PpsI GAGTC 1 cut(s) 1694
Ppu21I YACGTR 1 cut(s) 300
PsiI TTATAA 2 cut(s) 414, 1112
Psp124BI GAGCTC 2 cut(s) 207, 574
Psp1406I AACGTT 1 cut(s) 1431
Psp6I CCWGG 1 cut(s) 541
PspEI GGTNACC 1 cut(s) 620
PspFI CCCAGC 1 cut(s) 635
PspGI CCWGG 1 cut(s) 541
PspN4I GGNNCC 4 cut(s) 396, 539, 945, 1701
PspPI GGNCC 4 cut(s) 64, 176, 1555, 1624
PstNI CAGNNNCTG 2 cut(s) 382, 1763
PsyI GACNNNGTC 2 cut(s) 360, 715
PvuII CAGCTG 1 cut(s) 635
RsaI GTAC 6 cut(s) 553, 956, 1044, 1319, 1437, 1534
RsaNI GTAC 6 cut(s) 552, 955, 1043, 1318, 1436, 1533
RseI CAYNNNNRTG 2 cut(s) 302, 876
SacI GAGCTC 2 cut(s) 207, 574
SacII CCGCGG 1 cut(s) 825
SapI GCTCTTC 1 cut(s) 339
SaqAI TTAA 5 cut(s) 903, 1062, 1214, 1338, 1484
SatI GCNGC 5 cut(s) 499, 684, 825, 1395, 1398
Sau3AI GATC 5 cut(s) 35, 77, 231, 786, 1540
Sau96I GGNCC 4 cut(s) 64, 176, 1555, 1624
ScaI AGTACT 1 cut(s) 1319
SchI GAGTC 1 cut(s) 1695
ScrFI CCNGG 1 cut(s) 543
SduI GDGCHC 2 cut(s) 207, 574
SfaNI GCATC 2 cut(s) 487, 1269
Sfr303I CCGCGG 1 cut(s) 825
SfuI TTCGAA 1 cut(s) 1139
SgrBI CCGCGG 1 cut(s) 825
SinI GGWCC 4 cut(s) 64, 176, 1555, 1624
SmiMI CAYNNNNRTG 2 cut(s) 302, 876
SmlI CTYRAG 3 cut(s) 350, 1616, 1681
SmoI CTYRAG 3 cut(s) 350, 1616, 1681
Sse9I AATT 7 cut(s) 109, 145, 184, 505, 1274, 1467, 1784
SsiI CCGC 9 cut(s) 365, 401, 498, 778, 822, 824, 1395, 1553, 1627
SspMI CTAG 7 cut(s) 33, 336, 438, 675, 1008, 1103, 1601
SstI GAGCTC 2 cut(s) 207, 574
StyD4I CCNGG 1 cut(s) 541
TaaI ACNGT 6 cut(s) 362, 848, 1172, 1223, 1586, 1765
TaiI ACGT 2 cut(s) 302, 1434
TaqI TCGA 3 cut(s) 230, 372, 1139
TasI AATT 7 cut(s) 109, 145, 184, 505, 1274, 1467, 1784
TatI WGTACW 1 cut(s) 1317
TauI GCSGC 3 cut(s) 501, 827, 1397
TfiI GAWTC 5 cut(s) 328, 469, 774, 1312, 1670
Tru1I TTAA 5 cut(s) 903, 1062, 1214, 1338, 1484
Tru9I TTAA 5 cut(s) 903, 1062, 1214, 1338, 1484
TscAI CASTG 2 cut(s) 365, 851
TseFI GTSAC 3 cut(s) 287, 295, 620
TseI GCWGC 2 cut(s) 683, 1397
Tsp45I GTSAC 3 cut(s) 287, 295, 620
TspRI CASTG 2 cut(s) 365, 851
Tth111I GACNNNGTC 2 cut(s) 360, 715
VpaK11BI GGWCC 4 cut(s) 64, 176, 1555, 1624
XapI RAATTY 4 cut(s) 184, 1274, 1467, 1784
XbaI TCTAGA 1 cut(s) 335
XmnI GAANNNNTTC 3 cut(s) 109, 648, 1764
XspI CTAG 7 cut(s) 33, 336, 438, 675, 1008, 1103, 1601
ZrmI AGTACT 1 cut(s) 1319
Zsp2I ATGCAT 1 cut(s) 1378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.