Rh4AG245000

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0017813
Physical Location & Seq
Forward (+)
55579520 .. 55580516
997 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG245000.1

Sequence Viewer

Length: 285 bp
ATGGGTGATGAAGTTCGCGGACATTTTCGGCCGGGCATTATCTGCAGTGACTTCCGCCCAGTTTCCATGGGTCAAGATGTTCAAGGAGCCAACTGTGGTGAAGATTGCCAATCGATGGTATTGCAAAGAAAACCTGATGTCTTGATCATTATATTGCCGACATTGAGGGAAACTTCAAAGTATCAAGGACAAGATAACCTTCCAGTAATTGTATGGATGATAGTTCAAGTAAGTGGAATTTCACACAGGTTTGATAGAAGCTTCATGGTTAAAATACTATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

94

Amino Acids

10.59

Weight (kDa)

6.7

Isoelectric Point (pI)

37.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017813)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0017851
rosa_laevigata RLG00000020318 RLG00000030040
rosa_rugosa Rorug02G0253100 Rorug03G0048400
rosa_samantha Rh1BG268600 Rh1DG298700 Rh4AG245000 Rh4BG250200 Rh6BG057500 Rh6DG335800
rosa_wichuraiana Rw2G043450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 115
AccII CGCG 1 cut(s) 18
AciI CCGC 2 cut(s) 18, 55
AcoI YGGCCR 1 cut(s) 29
AcsI RAATTY 1 cut(s) 237
AfiI CCNNNNNNNGG 1 cut(s) 115
AgsI TTSAA 3 cut(s) 83, 177, 227
AluBI AGCT 1 cut(s) 261
AluI AGCT 1 cut(s) 261
AoxI GGCC 1 cut(s) 29
ApoI RAATTY 1 cut(s) 237
AsuC2I CCSGG 1 cut(s) 33
AsuHPI GGTGA 2 cut(s) 17, 110
BccI CCATC 1 cut(s) 109
BcgI CGANNNNNNTGC 2 cut(s) 103, 137
BclI TGATCA 1 cut(s) 144
BcnI CCSGG 1 cut(s) 33
BfmI CTRYAG 1 cut(s) 43
Bme1390I CCNGG 1 cut(s) 33
BmiI GGNNCC 1 cut(s) 88
BmrFI CCNGG 1 cut(s) 33
BmrI ACTGGG 1 cut(s) 53
BmuI ACTGGG 1 cut(s) 53
BpuMI CCSGG 1 cut(s) 33
Bsa29I ATCGAT 1 cut(s) 113
BsaJI CCNNGG 1 cut(s) 66
Bsc4I CCNNNNNNNGG 1 cut(s) 115
Bse1I ACTGG 2 cut(s) 59, 203
BseCI ATCGAT 1 cut(s) 113
BseDI CCNNGG 1 cut(s) 66
BseGI GGATG 1 cut(s) 222
BseLI CCNNNNNNNGG 1 cut(s) 115
BseNI ACTGG 2 cut(s) 59, 203
BseX3I CGGCCG 1 cut(s) 29
Bsh1236I CGCG 1 cut(s) 18
Bsh1285I CGRYCG 1 cut(s) 32
BshFI GGCC 1 cut(s) 31
BshVI ATCGAT 1 cut(s) 113
BsiEI CGRYCG 1 cut(s) 32
BsiSI CCGG 1 cut(s) 32
BslI CCNNNNNNNGG 1 cut(s) 115
BsnI GGCC 1 cut(s) 31
Bsp143I GATC 1 cut(s) 144
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 2 cut(s) 18, 55
BspANI GGCC 1 cut(s) 31
BspDI ATCGAT 1 cut(s) 113
BspFNI CGCG 1 cut(s) 18
BspLI GGNNCC 1 cut(s) 88
BspMAI CTGCAG 1 cut(s) 47
BsrI ACTGG 2 cut(s) 59, 203
BssECI CCNNGG 1 cut(s) 66
BssMI GATC 1 cut(s) 144
BssT1I CCWWGG 1 cut(s) 66
Bst4CI ACNGT 1 cut(s) 95
BstAPI GCANNNNNTGC 1 cut(s) 42
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 1 cut(s) 222
BstFNI CGCG 1 cut(s) 18
BstKTI GATC 1 cut(s) 147
BstMBI GATC 1 cut(s) 144
BstMCI CGRYCG 1 cut(s) 32
BstMWI GCNNNNNNNGC 1 cut(s) 42
BstSCI CCNGG 1 cut(s) 31
BstSFI CTRYAG 1 cut(s) 43
BstUI CGCG 1 cut(s) 18
BstZI CGGCCG 1 cut(s) 29
Bsu15I ATCGAT 1 cut(s) 113
BsuRI GGCC 1 cut(s) 31
BsuTUI ATCGAT 1 cut(s) 113
BtgI CCRYGG 1 cut(s) 66
BtsCI GGATG 1 cut(s) 222
BtsI GCAGTG 1 cut(s) 52
BtsIMutI CAGTG 1 cut(s) 52
ClaI ATCGAT 1 cut(s) 113
CviAII CATG 2 cut(s) 67, 265
CviJI RGCY 3 cut(s) 31, 89, 261
CviKI_1 RGCY 3 cut(s) 31, 89, 261
DpnI GATC 1 cut(s) 146
DpnII GATC 1 cut(s) 144
EaeI YGGCCR 1 cut(s) 29
EagI CGGCCG 1 cut(s) 29
EciI GGCGGA 1 cut(s) 44
EclXI CGGCCG 1 cut(s) 29
Eco130I CCWWGG 1 cut(s) 66
Eco52I CGGCCG 1 cut(s) 29
EcoT14I CCWWGG 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 66
FaeI CATG 2 cut(s) 70, 268
FaiI YATR 5 cut(s) 68, 152, 214, 266, 283
FalI AAGNNNNNCTT 2 cut(s) 183, 215
FatI CATG 2 cut(s) 66, 264
FbaI TGATCA 1 cut(s) 144
FokI GGATG 1 cut(s) 229
HaeIII GGCC 1 cut(s) 31
HapII CCGG 1 cut(s) 32
Hin1II CATG 2 cut(s) 70, 268
HindIII AAGCTT 1 cut(s) 259
HpaII CCGG 1 cut(s) 32
HphI GGTGA 2 cut(s) 17, 110
Hpy188III TCNNGA 2 cut(s) 74, 142
HpyAV CCTTC 1 cut(s) 209
HpyCH4III ACNGT 1 cut(s) 95
HpyCH4V TGCA 2 cut(s) 45, 124
HpyF10VI GCNNNNNNNGC 1 cut(s) 42
Hsp92II CATG 2 cut(s) 70, 268
Ksp22I TGATCA 1 cut(s) 144
Kzo9I GATC 1 cut(s) 144
LmnI GCTCC 1 cut(s) 86
LpnPI CCDG 5 cut(s) 45, 72, 147, 216, 232
MaeIII GTNAC 1 cut(s) 47
MalI GATC 1 cut(s) 146
MboI GATC 1 cut(s) 144
MboII GAAGA 1 cut(s) 113
MluCI AATT 2 cut(s) 207, 237
MnlI CCTC 1 cut(s) 159
MseI TTAA 1 cut(s) 270
MspI CCGG 1 cut(s) 32
MspR9I CCNGG 1 cut(s) 33
MvnI CGCG 1 cut(s) 18
MwoI GCNNNNNNNGC 1 cut(s) 42
NciI CCSGG 1 cut(s) 33
NcoI CCATGG 1 cut(s) 66
NdeII GATC 1 cut(s) 144
NlaIII CATG 2 cut(s) 70, 268
NlaIV GGNNCC 1 cut(s) 88
NmuCI GTSAC 1 cut(s) 47
PflMI CCANNNNNTGG 1 cut(s) 115
PspN4I GGNNCC 1 cut(s) 88
PstI CTGCAG 1 cut(s) 47
SaqAI TTAA 1 cut(s) 270
Sau3AI GATC 1 cut(s) 144
ScrFI CCNGG 1 cut(s) 33
SetI ASST 4 cut(s) 136, 201, 251, 263
SfcI CTRYAG 1 cut(s) 43
Sse9I AATT 2 cut(s) 207, 237
SsiI CCGC 2 cut(s) 18, 55
StyD4I CCNGG 1 cut(s) 31
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 1 cut(s) 95
TaqI TCGA 1 cut(s) 113
TasI AATT 2 cut(s) 207, 237
Tru1I TTAA 1 cut(s) 270
Tru9I TTAA 1 cut(s) 270
TscAI CASTG 1 cut(s) 52
TseFI GTSAC 1 cut(s) 47
Tsp45I GTSAC 1 cut(s) 47
TspDTI ATGAA 2 cut(s) 24, 253
TspRI CASTG 1 cut(s) 52
Van91I CCANNNNNTGG 1 cut(s) 115
XapI RAATTY 1 cut(s) 237
XcmI CCANNNNNNNNNTGG 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.