RLG00000030215

Belongs to the glycosyl hydrolase 2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0019289
Physical Location & Seq
Reverse (-)
57737078 .. 57744373
7296 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030215

Sequence Viewer

Length: 1233 bp
ATGGCTGCTTCTATGATGGGTCAACTGGTTTCTCCCTTGGAGAATGGACATCATGTGTGGGAGGACCAATCTTTCATCAAGTGGAAGAAGAGAGATGCACATGTCACTTTACGTTGCCATGAATCAATTGAAGGATCTCTTAAATACTGCTATGAACGCAACAAAGTGAATTTTATGGTATCCAACTCTGCACCTTGGAATGATGATGCTGTTCCTGAAGCTCTTGATAGTGCAGCTCTTTGGATCAAGGGCTTGCCTTTTGTGGAGTCTTTGTCAGGCTATTGGAAATTTTACTTAGCTTCCACTCCTGGAAATGTTCCTTTGAATTTTTACCACACCGCGTTTCAGGATTCTGAGTGGAAAACTTTGCCAGTTCCTTCAAATTGGCAGATGCATGGATTTGATCGGCCTATATATACTAATGTTGTATATCCATTTCCACTTGATCCACCTTTTGTTCCTGTGGACAACCCTACTGGCTGCTATAGGACAGATTTTGTCATCTCCAAAGAATGGAAGGGTCGCAGAATTTTGTTGCACTTCGAAGCTGTCGATTCTGCCTTCTGTGCATGGATAAATGGAGTTCCTGTAGGATATAGTCAGGACAGTCGACTTCCTGCTGAATTTGAAATCACTGATTATTGTTATCCTTGTGGGACGAACCAGAAAAATGTACTAGCTGTTCAAGTATTTAGATGGAGTGATGGCTCTTACCTTGAAGATCAGGATCATTGCTGGTTATCTGGCAGTCATCGTGATGTGCTTCCTCTTTCCAAGCCACAGATTATTTCTTCAAATCGAATCTGGCTGAGGATTTCTCTTATGCAGACATACAGGCAAGACTTTATTATTGAGGAGGATCTTTTTGTTTTCAACTTCTTGGTAGTGTTTTTGAAAATCGATAACTCCCAAGAAACATCTAAAGACAGTGTTCTTGATAATTTCACTATAGAAGCTGCATTATTTGACAGTGGGAACTGGTACAGTACTGGTGGATCTGCTGATCTGCTCTCATCAAATGTAGCTAATCTAAAGCTTGATCTCTCATCCAGCACAATTCCTGGATTTCGTGATTATTTGCTGGTAGGGAGACTGGAAGTGCCAAGGCTTTGGTCTGCAGAGCAGAGAAAAATTAAGTTGGAGAAAAGAGGAAGCACAGTTGTCATGGCCGACGGCTCAGTGGTTGCAGAACTGAATGCTTTGAGAGACAATGATCACTTGTTTATCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00511 ko00600 ko01100 map00052 map00511 map00600 map01100
Pfam Domains
Protein Families
Bgal_small_N DUF4981 Glyco_hydro_2 Glyco_hydro_2_C Glyco_hydro_2_N

Protein Analysis

411

Amino Acids

46.85

Weight (kDa)

5.25

Isoelectric Point (pI)

41.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_2_N PF02837 89 - 260 1e-50 Glycosyl hydrolases family 2, sugar binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019289)

Species Orthologous Gene IDs
malus_domestica MD07G1039900.v1.1 MD07G1040000.v1.1
rosa_chinensis RchiOBHm_Chr1g0324211
rosa_laevigata RLG00000030215
rosa_multiflora Rmu_sc0001850.1_g000039
rosa_samantha Rh1CG062400 Rh1CG062700 Rh1DG066500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 513
AccI GTMKAC 1 cut(s) 610
AccII CGCG 1 cut(s) 341
AciI CCGC 1 cut(s) 339
AclWI GGATC 6 cut(s) 142, 251, 440, 735, 867, 1003
AcoI YGGCCR 1 cut(s) 1167
AcsI RAATTY 5 cut(s) 169, 287, 325, 528, 623
AcuI CTGAAG 1 cut(s) 237
AfaI GTAC 3 cut(s) 675, 983, 988
AfiI CCNNNNNNNGG 1 cut(s) 513
AflIII ACRYGT 1 cut(s) 100
AgsI TTSAA 9 cut(s) 131, 325, 381, 629, 686, 719, 795, 874, 895
AjnI CCWGG 2 cut(s) 307, 1060
AluBI AGCT 8 cut(s) 221, 236, 299, 548, 680, 956, 1025, 1036
AluI AGCT 8 cut(s) 221, 236, 299, 548, 680, 956, 1025, 1036
Alw26I GTCTC 2 cut(s) 1084, 1200
AlwI GGATC 6 cut(s) 142, 251, 440, 735, 867, 1003
AoxI GGCC 2 cut(s) 407, 1167
ApeKI GCWGC 4 cut(s) 5, 233, 480, 956
ApoI RAATTY 5 cut(s) 169, 287, 325, 528, 623
AspS9I GGNCC 1 cut(s) 64
AsuII TTCGAA 1 cut(s) 543
AvaII GGWCC 1 cut(s) 64
BbvCI CCTCAGC 1 cut(s) 809
BbvI GCAGC 3 cut(s) 245, 467, 943
BccI CCATC 3 cut(s) 10, 690, 698
BceAI ACGGC 1 cut(s) 1189
BciT130I CCWGG 2 cut(s) 309, 1062
BciVI GTATCC 1 cut(s) 190
BclI TGATCA 1 cut(s) 1213
BcoDI GTCTC 2 cut(s) 1084, 1200
BfaI CTAG 1 cut(s) 677
BfmI CTRYAG 4 cut(s) 484, 588, 948, 1116
BfuI GTATCC 1 cut(s) 190
BisI GCNGC 4 cut(s) 6, 234, 481, 957
BlsI GCNGC 4 cut(s) 7, 235, 482, 958
BmcAI AGTACT 1 cut(s) 988
Bme1390I CCNGG 2 cut(s) 309, 1062
Bme18I GGWCC 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 64
BmrFI CCNGG 2 cut(s) 309, 1062
BmsI GCATC 3 cut(s) 85, 196, 381
BplI GAGNNNNNCTC 2 cut(s) 802, 834
Bpu10I CCTNAGC 1 cut(s) 809
Bpu14I TTCGAA 1 cut(s) 543
Bsa29I ATCGAT 1 cut(s) 900
BsaBI GATNNNNATC 1 cut(s) 726
BsaJI CCNNGG 3 cut(s) 36, 194, 1103
BsaXI ACNNNNNCTCC 4 cut(s) 257, 287, 573, 603
Bsc4I CCNNNNNNNGG 1 cut(s) 513
Bse1I ACTGG 6 cut(s) 30, 371, 481, 983, 994, 1098
Bse3DI GCAATG 1 cut(s) 730
Bse8I GATNNNNATC 1 cut(s) 726
BseBI CCWGG 2 cut(s) 309, 1062
BseCI ATCGAT 1 cut(s) 900
BseDI CCNNGG 3 cut(s) 36, 194, 1103
BseGI GGATG 1 cut(s) 1046
BseJI GATNNNNATC 1 cut(s) 726
BseLI CCNNNNNNNGG 1 cut(s) 513
BseMI GCAATG 1 cut(s) 730
BseMII CTCAG 3 cut(s) 345, 800, 1191
BseNI ACTGG 6 cut(s) 30, 371, 481, 983, 994, 1098
BseRI GAGGAG 1 cut(s) 869
BseXI GCAGC 3 cut(s) 245, 467, 943
BsgI GTGCAG 2 cut(s) 174, 252
Bsh1236I CGCG 1 cut(s) 341
BshFI GGCC 2 cut(s) 409, 1169
BshVI ATCGAT 1 cut(s) 900
BslFI GGGAC 1 cut(s) 670
BslI CCNNNNNNNGG 1 cut(s) 513
BsmAI GTCTC 2 cut(s) 1084, 1200
BsmFI GGGAC 1 cut(s) 670
BsmI GAATGC 1 cut(s) 1201
BsnI GGCC 2 cut(s) 409, 1169
Bsp119I TTCGAA 1 cut(s) 543
BspACI CCGC 1 cut(s) 339
BspANI GGCC 2 cut(s) 409, 1169
BspCNI CTCAG 3 cut(s) 346, 801, 1190
BspDI ATCGAT 1 cut(s) 900
BspFNI CGCG 1 cut(s) 341
BspMAI CTGCAG 1 cut(s) 1120
BspPI GGATC 6 cut(s) 142, 251, 440, 735, 867, 1003
BspT104I TTCGAA 1 cut(s) 543
BsrDI GCAATG 1 cut(s) 730
BsrI ACTGG 6 cut(s) 30, 371, 481, 983, 994, 1098
BssECI CCNNGG 3 cut(s) 36, 194, 1103
BssT1I CCWWGG 3 cut(s) 36, 194, 1103
Bst2UI CCWGG 2 cut(s) 309, 1062
Bst4CI ACNGT 5 cut(s) 608, 929, 971, 986, 1159
Bst6I CTCTTC 1 cut(s) 83
BstBI TTCGAA 1 cut(s) 543
BstC8I GCNNGC 1 cut(s) 254
BstDEI CTNAG 4 cut(s) 295, 354, 809, 1177
BstF5I GGATG 1 cut(s) 1046
BstFNI CGCG 1 cut(s) 341
BstMAI GTCTC 2 cut(s) 1084, 1200
BstMWI GCNNNNNNNGC 2 cut(s) 156, 566
BstNI CCWGG 2 cut(s) 309, 1062
BstNSI RCATGY 1 cut(s) 104
BstSCI CCNGG 2 cut(s) 307, 1060
BstSFI CTRYAG 4 cut(s) 484, 588, 948, 1116
BstUI CGCG 1 cut(s) 341
BstV1I GCAGC 3 cut(s) 245, 467, 943
BstX2I RGATCY 3 cut(s) 134, 859, 995
BstXI CCANNNNNNTGG 1 cut(s) 1110
BstYI RGATCY 3 cut(s) 134, 859, 995
Bsu15I ATCGAT 1 cut(s) 900
BsuI GTATCC 1 cut(s) 190
BsuRI GGCC 2 cut(s) 409, 1169
BsuTUI ATCGAT 1 cut(s) 900
BtsCI GGATG 1 cut(s) 1046
BtsIMutI CAGTG 4 cut(s) 633, 934, 976, 1185
Cac8I GCNNGC 1 cut(s) 254
Cfr13I GGNCC 1 cut(s) 64
ClaI ATCGAT 1 cut(s) 900
Csp6I GTAC 3 cut(s) 674, 982, 987
CviAII CATG 6 cut(s) 53, 101, 119, 395, 570, 1165
CviQI GTAC 3 cut(s) 674, 982, 987
DdeI CTNAG 4 cut(s) 295, 354, 809, 1177
EaeI YGGCCR 1 cut(s) 1167
Eam1104I CTCTTC 1 cut(s) 83
EarI CTCTTC 1 cut(s) 83
Eco130I CCWWGG 3 cut(s) 36, 194, 1103
Eco47I GGWCC 1 cut(s) 64
Eco57I CTGAAG 1 cut(s) 237
EcoRII CCWGG 2 cut(s) 307, 1060
EcoT14I CCWWGG 3 cut(s) 36, 194, 1103
EcoT22I ATGCAT 1 cut(s) 396
ErhI CCWWGG 3 cut(s) 36, 194, 1103
FaeI CATG 6 cut(s) 56, 104, 122, 398, 573, 1168
FalI AAGNNNNNCTT 2 cut(s) 123, 155
FaqI GGGAC 1 cut(s) 670
FatI CATG 6 cut(s) 52, 100, 118, 394, 569, 1164
FbaI TGATCA 1 cut(s) 1213
FblI GTMKAC 1 cut(s) 610
Fnu4HI GCNGC 4 cut(s) 6, 234, 481, 957
FokI GGATG 1 cut(s) 1033
Fsp4HI GCNGC 4 cut(s) 6, 234, 481, 957
FspBI CTAG 1 cut(s) 677
GluI GCNGC 4 cut(s) 6, 234, 481, 957
HaeIII GGCC 2 cut(s) 409, 1169
Hin1II CATG 6 cut(s) 56, 104, 122, 398, 573, 1168
HincII GTYRAC 2 cut(s) 23, 611
HindII GTYRAC 2 cut(s) 23, 611
HindIII AAGCTT 1 cut(s) 1034
HinfI GANTC 5 cut(s) 122, 266, 350, 554, 801
Hpy166II GTNNAC 3 cut(s) 23, 466, 611
Hpy188I TCNGA 1 cut(s) 355
Hpy188III TCNNGA 8 cut(s) 215, 224, 347, 602, 725, 755, 935, 1070
Hpy8I GTNNAC 3 cut(s) 23, 466, 611
Hpy99I CGWCG 1 cut(s) 1175
HpyAV CCTTC 4 cut(s) 125, 387, 511, 571
HpyCH4III ACNGT 5 cut(s) 608, 929, 971, 986, 1159
HpyCH4IV ACGT 1 cut(s) 112
HpyF10VI GCNNNNNNNGC 2 cut(s) 156, 566
HpyF3I CTNAG 4 cut(s) 295, 354, 809, 1177
HpySE526I ACGT 1 cut(s) 112
Hsp92II CATG 6 cut(s) 56, 104, 122, 398, 573, 1168
Ksp22I TGATCA 1 cut(s) 1213
Lsp1109I GCAGC 3 cut(s) 245, 467, 943
LweI GCATC 3 cut(s) 85, 196, 381
MaeI CTAG 1 cut(s) 677
MaeII ACGT 1 cut(s) 112
MaeIII GTNAC 1 cut(s) 103
MboII GAAGA 4 cut(s) 97, 100, 731, 783
MfeI CAATTG 1 cut(s) 126
MflI RGATCY 3 cut(s) 134, 859, 995
MlyI GAGTC 1 cut(s) 275
MmeI TCCRAC 2 cut(s) 207, 1119
MnlI CCTC 6 cut(s) 55, 777, 804, 847, 850, 1142
Mph1103I ATGCAT 1 cut(s) 396
MseI TTAA 2 cut(s) 141, 1134
MslI CAYNNNNRTG 1 cut(s) 756
MspR9I CCNGG 2 cut(s) 309, 1062
MunI CAATTG 1 cut(s) 126
Mva1269I GAATGC 1 cut(s) 1201
MvaI CCWGG 2 cut(s) 309, 1062
MvnI CGCG 1 cut(s) 341
MwoI GCNNNNNNNGC 2 cut(s) 156, 566
NlaIII CATG 6 cut(s) 56, 104, 122, 398, 573, 1168
NmuCI GTSAC 1 cut(s) 103
NsiI ATGCAT 1 cut(s) 396
NspI RCATGY 1 cut(s) 104
NspV TTCGAA 1 cut(s) 543
PciI ACATGT 1 cut(s) 100
PcsI WCGNNNNNNNCGW 1 cut(s) 549
PctI GAATGC 1 cut(s) 1201
PfeI GAWTC 4 cut(s) 122, 350, 554, 801
PflMI CCANNNNNTGG 1 cut(s) 513
PfoI TCCNGGA 2 cut(s) 307, 1060
PkrI GCNGC 4 cut(s) 7, 235, 482, 958
PleI GAGTC 1 cut(s) 274
PpsI GAGTC 1 cut(s) 274
PscI ACATGT 1 cut(s) 100
Psp6I CCWGG 2 cut(s) 307, 1060
PspGI CCWGG 2 cut(s) 307, 1060
PspPI GGNCC 1 cut(s) 64
PsrI GAACNNNNNNTAC 2 cut(s) 666, 698
PstI CTGCAG 1 cut(s) 1120
PsuI RGATCY 3 cut(s) 134, 859, 995
RsaI GTAC 3 cut(s) 675, 983, 988
RsaNI GTAC 3 cut(s) 674, 982, 987
RseI CAYNNNNRTG 1 cut(s) 756
SalI GTCGAC 1 cut(s) 609
SaqAI TTAA 2 cut(s) 141, 1134
SatI GCNGC 4 cut(s) 6, 234, 481, 957
Sau96I GGNCC 1 cut(s) 64
ScaI AGTACT 1 cut(s) 988
SchI GAGTC 1 cut(s) 275
ScrFI CCNGG 2 cut(s) 309, 1062
SfaNI GCATC 3 cut(s) 85, 196, 381
SfcI CTRYAG 4 cut(s) 484, 588, 948, 1116
SfuI TTCGAA 1 cut(s) 543
SinI GGWCC 1 cut(s) 64
SmiMI CAYNNNNRTG 1 cut(s) 756
SsiI CCGC 1 cut(s) 339
SspMI CTAG 1 cut(s) 677
StyD4I CCNGG 2 cut(s) 307, 1060
StyI CCWWGG 3 cut(s) 36, 194, 1103
TaaI ACNGT 5 cut(s) 608, 929, 971, 986, 1159
TaiI ACGT 1 cut(s) 115
TaqI TCGA 5 cut(s) 543, 552, 610, 799, 900
TatI WGTACW 2 cut(s) 673, 986
TfiI GAWTC 4 cut(s) 122, 350, 554, 801
Tru1I TTAA 2 cut(s) 141, 1134
Tru9I TTAA 2 cut(s) 141, 1134
TscAI CASTG 4 cut(s) 640, 934, 976, 1185
TseFI GTSAC 1 cut(s) 103
TseI GCWGC 4 cut(s) 5, 233, 480, 956
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 3 cut(s) 64, 135, 168
TspRI CASTG 4 cut(s) 640, 934, 976, 1185
Van91I CCANNNNNTGG 1 cut(s) 513
VpaK11BI GGWCC 1 cut(s) 64
XapI RAATTY 5 cut(s) 169, 287, 325, 528, 623
XceI RCATGY 1 cut(s) 104
XmiI GTMKAC 1 cut(s) 610
XspI CTAG 1 cut(s) 677
ZrmI AGTACT 1 cut(s) 988
Zsp2I ATGCAT 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.