Rh1CG062700

Belongs to the glycosyl hydrolase 2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0019289
Physical Location & Seq
Forward (+)
12349811 .. 12370905
21095 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG062700.1

Sequence Viewer

Length: 1362 bp
ATGGCTGCTTCTATGATGGGTCAACTGGTTTCTCCCTTGGAGAATGGGCATCATGTGTGGGAGGACCAATCTTTCATCAAGTGGAACAAGAGAGATGCACATGTCACTTTACGTTGCCATGAATCAATTGAAGGATCTCTTAAATACTGGTATGAACGCAACAAAGTGAATTTTATGGTATCCAACTCTGCACCTTGGAATGATGATGCTGTTCCTGAAGCTCTTGATAGTGCAGCTCTTTGGATCAAGGGCTTGCCTTTTGTGGAGTCTTTGTCAGGCTATTGGAAATTTTACTTAGCTTCCACTCCTGGAAATGTTCCTTTGAATTTTTACCACACCGCGTTTCAGGATTCTGAGTGGAAAACTTTGCCAGTTCCTTCAAATTGGCAGATGCACGGATTTGATCGGCCTATATATACTAATGTTGTATATCCATTTCCACTTGATCCACCTTTTGTTCCTGTGGACAACCCTACTGGCTGCTATAGGACAGATTTTGTGATCCCCAAAGAATGGAAGGGTCGCAGAATTTTGTTGCACTTCGAAGCTGTCGATTCTGCCTTCTGTGCATGGATAAATGGAGTTCCTGTAGGATATAGTCAGGACAGTCGACTTCCTGCTGAATTTGAAATCACTGATTATTGTTATCCTTGTGGGACGAACAAGAAAAATGTACTAGCTGTTCAACCACAGGTATTTATTGCAGATTATTTCTTCAAATCGAATCTGGCTGAGGATTTCTCTTATGCAGACATACAGGTTGAAGTGAAAATAGATAACTCCCAAGAAACATCTAAAGACAGTGTTCTTGATAATTTCACTATAGAAGCTGCATTATTTGACAGTGGGAACTGGTACAGTACTGGTGGATCTGCTGATCTGCTCTCGTCAAATGTAGCTAATCTAAAGCTTGATCTCTCATCCAGCACAATTCCTGGATTTCGTGATTATTTGCTGGTAGGGAGACTGGAAGTGCCAAGGCTTTGGTCTGCAGAGCAGGCGACTCTGGTTTCTCTTCTCCAAAAACAAACTCGCTTCGCTCTCTACGGACAAACCCTTTGCTTGAGGACCAAGCTCACCCTACGAGTGCTCAAAGTTGCTCAAATGGCTCCGACAGATTTCTCTGGTTCCTGCCGTTACTGCCCTGTGGAGTCTACTGACCTTTTGTCTCACATTGCTCTCAAGCACGGCAGGAAGTACTGTCAATCAAACACACACTTGCTGCTCCAAAAGCAAGTGTGTGCTTCCCATAACTCCAAAGGGAGAAGTGCATATCCATGTGGTTCAAGGTTTGAAGTGATTGGCTGCATTGGATCAGCTGGAGATTTTATCAGGAGAATATTAGCAGGAGATGGTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00511 ko00600 ko01100 map00052 map00511 map00600 map01100
Pfam Domains
Protein Families
Bgal_small_N DUF4981 Glyco_hydro_2 Glyco_hydro_2_C Glyco_hydro_2_N

Protein Analysis

453

Amino Acids

50.97

Weight (kDa)

6.14

Isoelectric Point (pI)

42.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_2_N PF02837 89 - 237 2.5e-37 Glycosyl hydrolases family 2, sugar binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019289)

Species Orthologous Gene IDs
malus_domestica MD07G1039900.v1.1 MD07G1040000.v1.1
rosa_chinensis RchiOBHm_Chr1g0324211
rosa_laevigata RLG00000030215
rosa_multiflora Rmu_sc0001850.1_g000039
rosa_samantha Rh1CG062400 Rh1CG062700 Rh1DG066500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 513
AccI GTMKAC 2 cut(s) 610, 1154
AccII CGCG 1 cut(s) 341
AciI CCGC 1 cut(s) 339
AclWI GGATC 6 cut(s) 142, 251, 440, 496, 877, 1321
AcsI RAATTY 5 cut(s) 169, 287, 325, 528, 623
AcuI CTGAAG 1 cut(s) 237
AfaI GTAC 4 cut(s) 675, 857, 862, 1199
AfiI CCNNNNNNNGG 1 cut(s) 513
AflIII ACRYGT 1 cut(s) 100
AgsI TTSAA 9 cut(s) 131, 325, 381, 629, 686, 718, 764, 1287, 1295
AhdI GACNNNNNGTC 1 cut(s) 1165
AjnI CCWGG 2 cut(s) 307, 934
Alw21I GWGCWC 1 cut(s) 1092
Alw26I GTCTC 2 cut(s) 958, 1173
AlwI GGATC 6 cut(s) 142, 251, 440, 496, 877, 1321
AoxI GGCC 1 cut(s) 407
ApeKI GCWGC 6 cut(s) 5, 233, 480, 830, 1222, 1305
ApoI RAATTY 5 cut(s) 169, 287, 325, 528, 623
ArsI GACNNNNNNTTYG 2 cut(s) 1041, 1073
AspS9I GGNCC 2 cut(s) 64, 1068
AsuHPI GGTGA 1 cut(s) 1069
AsuII TTCGAA 1 cut(s) 543
AvaII GGWCC 2 cut(s) 64, 1068
Bbv12I GWGCWC 1 cut(s) 1092
BbvCI CCTCAGC 1 cut(s) 732
BbvI GCAGC 5 cut(s) 245, 467, 817, 1209, 1292
BccI CCATC 2 cut(s) 10, 1346
BceAI ACGGC 2 cut(s) 1119, 1204
BciT130I CCWGG 2 cut(s) 309, 936
BciVI GTATCC 1 cut(s) 190
BcoDI GTCTC 2 cut(s) 958, 1173
BfaI CTAG 1 cut(s) 677
BfmI CTRYAG 4 cut(s) 484, 588, 822, 990
BfuI GTATCC 1 cut(s) 190
BisI GCNGC 6 cut(s) 6, 234, 481, 831, 1223, 1306
BlsI GCNGC 6 cut(s) 7, 235, 482, 832, 1224, 1307
BmcAI AGTACT 2 cut(s) 862, 1199
Bme1390I CCNGG 2 cut(s) 309, 936
Bme18I GGWCC 2 cut(s) 64, 1068
BmeRI GACNNNNNGTC 1 cut(s) 1165
BmgT120I GGNCC 2 cut(s) 64, 1068
BmiI GGNNCC 2 cut(s) 1110, 1129
BmrFI CCNGG 2 cut(s) 309, 936
BmsI GCATC 4 cut(s) 58, 85, 196, 381
BplI GAGNNNNNCTC 2 cut(s) 725, 757
BpmI CTGGAG 1 cut(s) 1341
Bpu10I CCTNAGC 1 cut(s) 732
Bpu14I TTCGAA 1 cut(s) 543
BpuEI CTTGAG 2 cut(s) 1084, 1166
BsaJI CCNNGG 3 cut(s) 36, 194, 977
BsaXI ACNNNNNCTCC 4 cut(s) 257, 287, 573, 603
Bsc4I CCNNNNNNNGG 1 cut(s) 513
Bse1I ACTGG 7 cut(s) 30, 152, 371, 481, 857, 868, 972
Bse3DI GCAATG 1 cut(s) 1173
BseBI CCWGG 2 cut(s) 309, 936
BseDI CCNNGG 3 cut(s) 36, 194, 977
BseGI GGATG 1 cut(s) 920
BseLI CCNNNNNNNGG 1 cut(s) 513
BseMI GCAATG 1 cut(s) 1173
BseMII CTCAG 2 cut(s) 345, 723
BseNI ACTGG 7 cut(s) 30, 152, 371, 481, 857, 868, 972
BseXI GCAGC 5 cut(s) 245, 467, 817, 1209, 1292
BsgI GTGCAG 2 cut(s) 174, 252
Bsh1236I CGCG 1 cut(s) 341
BshFI GGCC 1 cut(s) 409
BsiHKAI GWGCWC 1 cut(s) 1092
BslFI GGGAC 1 cut(s) 670
BslI CCNNNNNNNGG 1 cut(s) 513
BsmAI GTCTC 2 cut(s) 958, 1173
BsmFI GGGAC 1 cut(s) 670
BsnI GGCC 1 cut(s) 409
Bsp119I TTCGAA 1 cut(s) 543
Bsp1286I GDGCHC 1 cut(s) 1092
Bsp143I GATC 9 cut(s) 134, 243, 403, 445, 501, 869, 877, 913, 1313
BspACI CCGC 1 cut(s) 339
BspANI GGCC 1 cut(s) 409
BspCNI CTCAG 2 cut(s) 346, 724
BspFNI CGCG 1 cut(s) 341
BspLI GGNNCC 2 cut(s) 1110, 1129
BspMAI CTGCAG 1 cut(s) 994
BspPI GGATC 6 cut(s) 142, 251, 440, 496, 877, 1321
BspT104I TTCGAA 1 cut(s) 543
BsrDI GCAATG 1 cut(s) 1173
BsrI ACTGG 7 cut(s) 30, 152, 371, 481, 857, 868, 972
BssECI CCNNGG 3 cut(s) 36, 194, 977
BssMI GATC 9 cut(s) 134, 243, 403, 445, 501, 869, 877, 913, 1313
BssT1I CCWWGG 3 cut(s) 36, 194, 977
Bst2UI CCWGG 2 cut(s) 309, 936
Bst4CI ACNGT 5 cut(s) 608, 803, 845, 860, 1202
Bst6I CTCTTC 1 cut(s) 1020
BstBI TTCGAA 1 cut(s) 543
BstC8I GCNNGC 2 cut(s) 254, 999
BstDEI CTNAG 3 cut(s) 295, 354, 732
BstF5I GGATG 1 cut(s) 920
BstFNI CGCG 1 cut(s) 341
BstKTI GATC 9 cut(s) 137, 246, 406, 448, 504, 872, 880, 916, 1316
BstMAI GTCTC 2 cut(s) 958, 1173
BstMBI GATC 9 cut(s) 134, 243, 403, 445, 501, 869, 877, 913, 1313
BstMWI GCNNNNNNNGC 5 cut(s) 566, 998, 1106, 1140, 1231
BstNI CCWGG 2 cut(s) 309, 936
BstNSI RCATGY 1 cut(s) 104
BstSCI CCNGG 2 cut(s) 307, 934
BstSFI CTRYAG 4 cut(s) 484, 588, 822, 990
BstUI CGCG 1 cut(s) 341
BstV1I GCAGC 5 cut(s) 245, 467, 817, 1209, 1292
BstX2I RGATCY 2 cut(s) 134, 869
BstXI CCANNNNNNTGG 1 cut(s) 984
BstYI RGATCY 2 cut(s) 134, 869
BsuI GTATCC 1 cut(s) 190
BsuRI GGCC 1 cut(s) 409
BtsCI GGATG 1 cut(s) 920
BtsIMutI CAGTG 3 cut(s) 633, 808, 850
Cac8I GCNNGC 2 cut(s) 254, 999
Cfr13I GGNCC 2 cut(s) 64, 1068
Csp6I GTAC 4 cut(s) 674, 856, 861, 1198
CviAII CATG 5 cut(s) 53, 101, 119, 570, 1278
CviQI GTAC 4 cut(s) 674, 856, 861, 1198
DdeI CTNAG 3 cut(s) 295, 354, 732
DpnI GATC 9 cut(s) 136, 245, 405, 447, 503, 871, 879, 915, 1315
DpnII GATC 9 cut(s) 134, 243, 403, 445, 501, 869, 877, 913, 1313
DriI GACNNNNNGTC 1 cut(s) 1165
Eam1104I CTCTTC 1 cut(s) 1020
Eam1105I GACNNNNNGTC 1 cut(s) 1165
EarI CTCTTC 1 cut(s) 1020
Eco130I CCWWGG 3 cut(s) 36, 194, 977
Eco47I GGWCC 2 cut(s) 64, 1068
Eco57I CTGAAG 1 cut(s) 237
EcoRII CCWGG 2 cut(s) 307, 934
EcoT14I CCWWGG 3 cut(s) 36, 194, 977
ErhI CCWWGG 3 cut(s) 36, 194, 977
FaeI CATG 5 cut(s) 56, 104, 122, 573, 1281
FalI AAGNNNNNCTT 2 cut(s) 123, 155
FaqI GGGAC 1 cut(s) 670
FatI CATG 5 cut(s) 52, 100, 118, 569, 1277
FblI GTMKAC 2 cut(s) 610, 1154
Fnu4HI GCNGC 6 cut(s) 6, 234, 481, 831, 1223, 1306
FokI GGATG 1 cut(s) 907
Fsp4HI GCNGC 6 cut(s) 6, 234, 481, 831, 1223, 1306
FspBI CTAG 1 cut(s) 677
GluI GCNGC 6 cut(s) 6, 234, 481, 831, 1223, 1306
GsuI CTGGAG 1 cut(s) 1341
HaeIII GGCC 1 cut(s) 409
Hin1II CATG 5 cut(s) 56, 104, 122, 573, 1281
HincII GTYRAC 2 cut(s) 23, 611
HindII GTYRAC 2 cut(s) 23, 611
HindIII AAGCTT 1 cut(s) 908
HinfI GANTC 7 cut(s) 122, 266, 350, 554, 724, 1003, 1151
HphI GGTGA 1 cut(s) 1069
Hpy166II GTNNAC 4 cut(s) 23, 466, 611, 1155
Hpy188I TCNGA 2 cut(s) 355, 1113
Hpy188III TCNNGA 7 cut(s) 215, 224, 347, 602, 809, 944, 1333
Hpy8I GTNNAC 4 cut(s) 23, 466, 611, 1155
HpyAV CCTTC 4 cut(s) 125, 387, 511, 571
HpyCH4III ACNGT 5 cut(s) 608, 803, 845, 860, 1202
HpyCH4IV ACGT 1 cut(s) 112
HpyF10VI GCNNNNNNNGC 5 cut(s) 566, 998, 1106, 1140, 1231
HpyF3I CTNAG 3 cut(s) 295, 354, 732
HpySE526I ACGT 1 cut(s) 112
Hsp92II CATG 5 cut(s) 56, 104, 122, 573, 1281
Kzo9I GATC 9 cut(s) 134, 243, 403, 445, 501, 869, 877, 913, 1313
LmnI GCTCC 2 cut(s) 1114, 1230
Lsp1109I GCAGC 5 cut(s) 245, 467, 817, 1209, 1292
LweI GCATC 4 cut(s) 58, 85, 196, 381
MaeI CTAG 1 cut(s) 677
MaeII ACGT 1 cut(s) 112
MaeIII GTNAC 2 cut(s) 103, 1136
MalI GATC 9 cut(s) 136, 245, 405, 447, 503, 871, 879, 915, 1315
MboI GATC 9 cut(s) 134, 243, 403, 445, 501, 869, 877, 913, 1313
MboII GAAGA 2 cut(s) 706, 1007
MfeI CAATTG 1 cut(s) 126
MflI RGATCY 2 cut(s) 134, 869
MhlI GDGCHC 1 cut(s) 1092
MluCI AATT 9 cut(s) 126, 169, 287, 325, 382, 528, 623, 814, 930
MlyI GAGTC 3 cut(s) 275, 997, 1160
MmeI TCCRAC 2 cut(s) 207, 1136
MnlI CCTC 3 cut(s) 55, 727, 1059
MseI TTAA 1 cut(s) 141
MslI CAYNNNNRTG 1 cut(s) 1276
MspA1I CMGCKG 1 cut(s) 1319
MspR9I CCNGG 2 cut(s) 309, 936
MunI CAATTG 1 cut(s) 126
MvaI CCWGG 2 cut(s) 309, 936
MvnI CGCG 1 cut(s) 341
MwoI GCNNNNNNNGC 5 cut(s) 566, 998, 1106, 1140, 1231
NdeII GATC 9 cut(s) 134, 243, 403, 445, 501, 869, 877, 913, 1313
NlaIII CATG 5 cut(s) 56, 104, 122, 573, 1281
NlaIV GGNNCC 2 cut(s) 1110, 1129
NmuCI GTSAC 1 cut(s) 103
NspI RCATGY 1 cut(s) 104
NspV TTCGAA 1 cut(s) 543
PciI ACATGT 1 cut(s) 100
PcsI WCGNNNNNNNCGW 1 cut(s) 549
PfeI GAWTC 4 cut(s) 122, 350, 554, 724
PflMI CCANNNNNTGG 1 cut(s) 513
PfoI TCCNGGA 2 cut(s) 307, 934
PkrI GCNGC 6 cut(s) 7, 235, 482, 832, 1224, 1307
PleI GAGTC 3 cut(s) 274, 997, 1159
PpsI GAGTC 3 cut(s) 274, 997, 1159
PscI ACATGT 1 cut(s) 100
Psp6I CCWGG 2 cut(s) 307, 934
PspGI CCWGG 2 cut(s) 307, 934
PspN4I GGNNCC 2 cut(s) 1110, 1129
PspPI GGNCC 2 cut(s) 64, 1068
PsrI GAACNNNNNNTAC 2 cut(s) 666, 698
PstI CTGCAG 1 cut(s) 994
PsuI RGATCY 2 cut(s) 134, 869
PvuII CAGCTG 1 cut(s) 1319
RsaI GTAC 4 cut(s) 675, 857, 862, 1199
RsaNI GTAC 4 cut(s) 674, 856, 861, 1198
RseI CAYNNNNRTG 1 cut(s) 1276
SalI GTCGAC 1 cut(s) 609
SaqAI TTAA 1 cut(s) 141
SatI GCNGC 6 cut(s) 6, 234, 481, 831, 1223, 1306
Sau3AI GATC 9 cut(s) 134, 243, 403, 445, 501, 869, 877, 913, 1313
Sau96I GGNCC 2 cut(s) 64, 1068
ScaI AGTACT 2 cut(s) 862, 1199
SchI GAGTC 3 cut(s) 275, 997, 1160
ScrFI CCNGG 2 cut(s) 309, 936
SduI GDGCHC 1 cut(s) 1092
SfaNI GCATC 4 cut(s) 58, 85, 196, 381
SfcI CTRYAG 4 cut(s) 484, 588, 822, 990
SfuI TTCGAA 1 cut(s) 543
SinI GGWCC 2 cut(s) 64, 1068
SmiMI CAYNNNNRTG 1 cut(s) 1276
SmlI CTYRAG 2 cut(s) 1063, 1181
SmoI CTYRAG 2 cut(s) 1063, 1181
Sse9I AATT 9 cut(s) 126, 169, 287, 325, 382, 528, 623, 814, 930
SsiI CCGC 1 cut(s) 339
SspI AATATT 1 cut(s) 1341
SspMI CTAG 1 cut(s) 677
StyD4I CCNGG 2 cut(s) 307, 934
StyI CCWWGG 3 cut(s) 36, 194, 977
TaaI ACNGT 5 cut(s) 608, 803, 845, 860, 1202
TaiI ACGT 1 cut(s) 115
TaqI TCGA 4 cut(s) 543, 552, 610, 722
TasI AATT 9 cut(s) 126, 169, 287, 325, 382, 528, 623, 814, 930
TatI WGTACW 3 cut(s) 673, 860, 1197
TfiI GAWTC 4 cut(s) 122, 350, 554, 724
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TscAI CASTG 3 cut(s) 640, 808, 850
TseFI GTSAC 1 cut(s) 103
TseI GCWGC 6 cut(s) 5, 233, 480, 830, 1222, 1305
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 3 cut(s) 64, 135, 168
TspGWI ACGGA 2 cut(s) 411, 1062
TspRI CASTG 3 cut(s) 640, 808, 850
Van91I CCANNNNNTGG 1 cut(s) 513
VpaK11BI GGWCC 2 cut(s) 64, 1068
XapI RAATTY 5 cut(s) 169, 287, 325, 528, 623
XceI RCATGY 1 cut(s) 104
XmiI GTMKAC 2 cut(s) 610, 1154
XspI CTAG 1 cut(s) 677
ZrmI AGTACT 2 cut(s) 862, 1199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.