Rh1CG062400

Belongs to the glycosyl hydrolase 2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0019289
Physical Location & Seq
Forward (+)
12311074 .. 12313983
2910 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG062400.1

Sequence Viewer

Length: 570 bp
ATGACGGGAATGAAGTTTTCGTTTGCAGAGATCTATACTTTAGATGTCAGTAATTACCTTTTGGCAGGTCGCAGAATTTTGTTGCACTTCGAAGCTGTCGATTCTGCCTTCTGTGCATGGATAAATGGAGTTCCTGTAGGATATAGTCAGGACAGTCGACTTCCTGCTGAATTTGAAATCACTGATTATTGTTATCCTTGTGGGACGAACAAGAAAAATGTACTAGCTGTTCAACCACAGGTATTTATTGCAGATTATTTCTTCAAATCGAATCTGGCTGAGGATTTCTCTTATGCAGACATACAGGTTGAAGTGAAAATAGATAACTCCCAAGAAACATCTAAAGACAGTGTTCTTGATAATTTCACTATAGAAGCTGCATTATTTGACAGTGGGAACTGGTACAGTACTGGTGGATCTGCTGATCTGCTCTCGTCAAATGTAGCTAATCTAAAGCTTGATCTCTCATCCAGCACAATTCCTGGATTTCGTGATTATTTGCTGGTAGGGAGACTGGAAGTGCCAAGGCTTTGGTCTGCAGAGCAGGAGCAGCAAAATGATACAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00052 ko00511 ko00600 ko01100 map00052 map00511 map00600 map01100
Pfam Domains
Protein Families
Bgal_small_N DUF4981 Glyco_hydro_2 Glyco_hydro_2_C Glyco_hydro_2_N

Protein Analysis

189

Amino Acids

21.14

Weight (kDa)

4.3

Isoelectric Point (pI)

33.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_2_N PF02837 14 - 86 1.1e-09 Glycosyl hydrolases family 2, sugar binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019289)

Species Orthologous Gene IDs
malus_domestica MD07G1039900.v1.1 MD07G1040000.v1.1
rosa_chinensis RchiOBHm_Chr1g0324211
rosa_laevigata RLG00000030215
rosa_multiflora Rmu_sc0001850.1_g000039
rosa_samantha Rh1CG062400 Rh1CG062700 Rh1DG066500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 56
AccI GTMKAC 1 cut(s) 157
AclWI GGATC 1 cut(s) 424
AcsI RAATTY 2 cut(s) 75, 170
AfaI GTAC 3 cut(s) 222, 404, 409
AgsI TTSAA 4 cut(s) 176, 233, 265, 311
AjnI CCWGG 1 cut(s) 481
AluBI AGCT 5 cut(s) 95, 227, 377, 446, 457
AluI AGCT 5 cut(s) 95, 227, 377, 446, 457
Alw26I GTCTC 1 cut(s) 505
AlwI GGATC 1 cut(s) 424
ApeKI GCWGC 2 cut(s) 377, 550
ApoI RAATTY 2 cut(s) 75, 170
AsuII TTCGAA 1 cut(s) 90
BbvCI CCTCAGC 1 cut(s) 279
BbvI GCAGC 2 cut(s) 364, 562
BciT130I CCWGG 1 cut(s) 483
BcoDI GTCTC 1 cut(s) 505
BfaI CTAG 1 cut(s) 224
BfmI CTRYAG 3 cut(s) 135, 369, 537
BfuAI ACCTGC 1 cut(s) 56
BglII AGATCT 1 cut(s) 30
BisI GCNGC 2 cut(s) 378, 551
BlsI GCNGC 2 cut(s) 379, 552
BmcAI AGTACT 1 cut(s) 409
Bme1390I CCNGG 1 cut(s) 483
BmrFI CCNGG 1 cut(s) 483
BplI GAGNNNNNCTC 2 cut(s) 272, 304
Bpu10I CCTNAGC 1 cut(s) 279
Bpu14I TTCGAA 1 cut(s) 90
BsaJI CCNNGG 1 cut(s) 524
BsaXI ACNNNNNCTCC 2 cut(s) 120, 150
Bse1I ACTGG 3 cut(s) 404, 415, 519
BseBI CCWGG 1 cut(s) 483
BseDI CCNNGG 1 cut(s) 524
BseGI GGATG 1 cut(s) 467
BseMII CTCAG 1 cut(s) 270
BseNI ACTGG 3 cut(s) 404, 415, 519
BseXI GCAGC 2 cut(s) 364, 562
BslFI GGGAC 1 cut(s) 217
BsmAI GTCTC 1 cut(s) 505
BsmFI GGGAC 1 cut(s) 217
Bsp119I TTCGAA 1 cut(s) 90
Bsp143I GATC 4 cut(s) 30, 416, 424, 460
BspCNI CTCAG 1 cut(s) 271
BspMAI CTGCAG 1 cut(s) 541
BspMI ACCTGC 1 cut(s) 56
BspPI GGATC 1 cut(s) 424
BspT104I TTCGAA 1 cut(s) 90
BsrI ACTGG 3 cut(s) 404, 415, 519
BssECI CCNNGG 1 cut(s) 524
BssMI GATC 4 cut(s) 30, 416, 424, 460
BssT1I CCWWGG 1 cut(s) 524
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 4 cut(s) 155, 350, 392, 407
BstBI TTCGAA 1 cut(s) 90
BstDEI CTNAG 1 cut(s) 279
BstF5I GGATG 1 cut(s) 467
BstKTI GATC 4 cut(s) 33, 419, 427, 463
BstMAI GTCTC 1 cut(s) 505
BstMBI GATC 4 cut(s) 30, 416, 424, 460
BstMWI GCNNNNNNNGC 2 cut(s) 113, 550
BstNI CCWGG 1 cut(s) 483
BstSCI CCNGG 1 cut(s) 481
BstSFI CTRYAG 3 cut(s) 135, 369, 537
BstV1I GCAGC 2 cut(s) 364, 562
BstX2I RGATCY 2 cut(s) 30, 416
BstXI CCANNNNNNTGG 1 cut(s) 531
BstYI RGATCY 2 cut(s) 30, 416
BtsCI GGATG 1 cut(s) 467
BtsIMutI CAGTG 3 cut(s) 180, 355, 397
BveI ACCTGC 1 cut(s) 56
Csp6I GTAC 3 cut(s) 221, 403, 408
CviAII CATG 1 cut(s) 117
CviJI RGCY 7 cut(s) 95, 227, 278, 377, 446, 457, 529
CviKI_1 RGCY 7 cut(s) 95, 227, 278, 377, 446, 457, 529
CviQI GTAC 3 cut(s) 221, 403, 408
DdeI CTNAG 1 cut(s) 279
DpnI GATC 4 cut(s) 32, 418, 426, 462
DpnII GATC 4 cut(s) 30, 416, 424, 460
Eco130I CCWWGG 1 cut(s) 524
EcoRII CCWGG 1 cut(s) 481
EcoT14I CCWWGG 1 cut(s) 524
ErhI CCWWGG 1 cut(s) 524
FaeI CATG 1 cut(s) 120
FaiI YATR 6 cut(s) 36, 118, 144, 294, 302, 371
FaqI GGGAC 1 cut(s) 217
FatI CATG 1 cut(s) 116
FblI GTMKAC 1 cut(s) 157
Fnu4HI GCNGC 2 cut(s) 378, 551
FokI GGATG 1 cut(s) 454
Fsp4HI GCNGC 2 cut(s) 378, 551
FspBI CTAG 1 cut(s) 224
GluI GCNGC 2 cut(s) 378, 551
Hin1II CATG 1 cut(s) 120
HincII GTYRAC 1 cut(s) 158
HindII GTYRAC 1 cut(s) 158
HindIII AAGCTT 1 cut(s) 455
HinfI GANTC 2 cut(s) 101, 271
Hpy166II GTNNAC 1 cut(s) 158
Hpy188III TCNNGA 3 cut(s) 149, 356, 491
Hpy8I GTNNAC 1 cut(s) 158
HpyAV CCTTC 1 cut(s) 118
HpyCH4III ACNGT 4 cut(s) 155, 350, 392, 407
HpyCH4V TGCA 7 cut(s) 26, 85, 116, 251, 296, 380, 539
HpyF10VI GCNNNNNNNGC 2 cut(s) 113, 550
HpyF3I CTNAG 1 cut(s) 279
Hsp92II CATG 1 cut(s) 120
Kzo9I GATC 4 cut(s) 30, 416, 424, 460
LmnI GCTCC 1 cut(s) 547
Lsp1109I GCAGC 2 cut(s) 364, 562
MaeI CTAG 1 cut(s) 224
MalI GATC 4 cut(s) 32, 418, 426, 462
MboI GATC 4 cut(s) 30, 416, 424, 460
MboII GAAGA 1 cut(s) 253
MflI RGATCY 2 cut(s) 30, 416
MluCI AATT 6 cut(s) 52, 75, 170, 361, 477, 565
MnlI CCTC 1 cut(s) 274
MseI TTAA 1 cut(s) 568
MspR9I CCNGG 1 cut(s) 483
MvaI CCWGG 1 cut(s) 483
MwoI GCNNNNNNNGC 2 cut(s) 113, 550
NdeII GATC 4 cut(s) 30, 416, 424, 460
NlaIII CATG 1 cut(s) 120
NspV TTCGAA 1 cut(s) 90
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PfeI GAWTC 2 cut(s) 101, 271
PfoI TCCNGGA 1 cut(s) 481
PkrI GCNGC 2 cut(s) 379, 552
Psp6I CCWGG 1 cut(s) 481
PspGI CCWGG 1 cut(s) 481
PsrI GAACNNNNNNTAC 2 cut(s) 213, 245
PstI CTGCAG 1 cut(s) 541
PsuI RGATCY 2 cut(s) 30, 416
RsaI GTAC 3 cut(s) 222, 404, 409
RsaNI GTAC 3 cut(s) 221, 403, 408
SalI GTCGAC 1 cut(s) 156
SaqAI TTAA 1 cut(s) 568
SatI GCNGC 2 cut(s) 378, 551
Sau3AI GATC 4 cut(s) 30, 416, 424, 460
ScaI AGTACT 1 cut(s) 409
ScrFI CCNGG 1 cut(s) 483
SetI ASST 9 cut(s) 60, 70, 97, 229, 243, 309, 379, 448, 459
SfcI CTRYAG 3 cut(s) 135, 369, 537
SfuI TTCGAA 1 cut(s) 90
Sse9I AATT 6 cut(s) 52, 75, 170, 361, 477, 565
SspMI CTAG 1 cut(s) 224
StyD4I CCNGG 1 cut(s) 481
StyI CCWWGG 1 cut(s) 524
TaaI ACNGT 4 cut(s) 155, 350, 392, 407
TaqI TCGA 4 cut(s) 90, 99, 157, 269
TasI AATT 6 cut(s) 52, 75, 170, 361, 477, 565
TatI WGTACW 2 cut(s) 220, 407
TfiI GAWTC 2 cut(s) 101, 271
Tru1I TTAA 1 cut(s) 568
Tru9I TTAA 1 cut(s) 568
TscAI CASTG 3 cut(s) 187, 355, 397
TseI GCWGC 2 cut(s) 377, 550
TspDTI ATGAA 1 cut(s) 26
TspRI CASTG 3 cut(s) 187, 355, 397
XapI RAATTY 2 cut(s) 75, 170
XmiI GTMKAC 1 cut(s) 157
XspI CTAG 1 cut(s) 224
ZrmI AGTACT 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.