RLG00000033293

Olee1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0016288
Physical Location & Seq
Forward (+)
25366501 .. 25367117
617 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033293

Sequence Viewer

Length: 501 bp
ATGGCCCAGACTTTGGGAGTCACAGCTGTTGTTCTCACCGTATTTTGCTTGTGCTCTTTGGCGAATGCCGACGGCGGCAGAATTAGTGAAATCTCTGTCGAAGGCAAGGTGTACTGTGATCCCTGCCGTGTCCAATTCCAAACCAGAATCAGCGAACCCATCGAAGGTGCCACAGTAGCCTTGCAATGCAGGAGCCGCATAAATTCAACCGTCTTATACACAGTTGAACGCAAGACTGGTAAGGATGGCCTTTACACTATTCCCGTGGATGAGGACTACGCAGAGGAGATCTGCGAGGTGAAGACACTGAGCAGCCCTAGGGCGGATTGCAACGACCGTTTCGATAAGTTTGAGAAGGCTAGAGTTTTGATCACATACAACAATGGTGTTGCCTCCACGGCTCGTTATGCCAATCCCCTTGGGTTCATGAAGAAGGAAGCAGTTCCTGAGTGCGAGAAAGTACTCAAGGAACTGTTTCCCGAGGAGCAGACCGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

167

Amino Acids

18.43

Weight (kDa)

4.9

Isoelectric Point (pI)

37.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 33 - 113 2.9e-17 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016288)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g18470
malus_domestica MD03G1241700.v1.1 MD11G1261000.v1.1
prunus_persica Prupe.4G167200_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0031231
rosa_laevigata RLG00000033293
rosa_multiflora Rmu_sc0004014.1_g000008
rosa_roxburghii Rroxscaffold_1G00049050
rosa_rugosa Rorug05G0123000
rosa_samantha Rh5AG216600 Rh5BG216000 Rh5CG239800 Rh5DG221100
rosa_wichuraiana Rw0G010930 Rw5G019810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 167
AccB7I CCANNNNNTGG 1 cut(s) 13
AciI CCGC 3 cut(s) 75, 196, 323
AclWI GGATC 1 cut(s) 113
AcsI RAATTY 1 cut(s) 202
AfaI GTAC 2 cut(s) 113, 462
AfiI CCNNNNNNNGG 3 cut(s) 13, 164, 322
AgsI TTSAA 2 cut(s) 207, 227
AluBI AGCT 1 cut(s) 26
AluI AGCT 1 cut(s) 26
Alw21I GWGCWC 1 cut(s) 56
AlwI GGATC 1 cut(s) 113
AlwNI CAGNNNCTG 1 cut(s) 446
Ama87I CYCGRG 1 cut(s) 479
AoxI GGCC 2 cut(s) 3, 247
ApeKI GCWGC 1 cut(s) 312
ApoI RAATTY 1 cut(s) 202
Asp700I GAANNNNTTC 2 cut(s) 441, 474
AspA2I CCTAGG 1 cut(s) 317
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 2 cut(s) 28, 310
AvaI CYCGRG 1 cut(s) 479
AvrII CCTAGG 1 cut(s) 317
BanI GGYRCC 1 cut(s) 167
BbsI GAAGAC 1 cut(s) 308
Bbv12I GWGCWC 1 cut(s) 56
BbvI GCAGC 1 cut(s) 324
BccI CCATC 2 cut(s) 167, 239
BceAI ACGGC 3 cut(s) 88, 111, 414
BclI TGATCA 1 cut(s) 369
BfaI CTAG 2 cut(s) 318, 360
BglI GCCNNNNNGGC 1 cut(s) 398
BglII AGATCT 1 cut(s) 288
BisI GCNGC 3 cut(s) 76, 196, 313
BlnI CCTAGG 1 cut(s) 317
BlsI GCNGC 3 cut(s) 77, 197, 314
BmcAI AGTACT 1 cut(s) 462
BmeT110I CYCGRG 1 cut(s) 479
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 2 cut(s) 169, 194
BpiI GAAGAC 1 cut(s) 308
BpuEI CTTGAG 1 cut(s) 449
BsaJI CCNNGG 5 cut(s) 264, 317, 396, 418, 480
Bsc4I CCNNNNNNNGG 3 cut(s) 13, 164, 322
Bse1I ACTGG 1 cut(s) 241
Bse3DI GCAATG 1 cut(s) 191
BseDI CCNNGG 5 cut(s) 264, 317, 396, 418, 480
BseGI GGATG 2 cut(s) 250, 274
BseLI CCNNNNNNNGG 3 cut(s) 13, 164, 322
BseMI GCAATG 1 cut(s) 191
BseMII CTCAG 2 cut(s) 299, 438
BseNI ACTGG 1 cut(s) 241
BseRI GAGGAG 2 cut(s) 299, 497
BseXI GCAGC 1 cut(s) 324
Bsh1285I CGRYCG 1 cut(s) 337
BshFI GGCC 2 cut(s) 5, 249
BshNI GGYRCC 1 cut(s) 167
BsiEI CGRYCG 1 cut(s) 337
BsiHKAI GWGCWC 1 cut(s) 56
BsiHKCI CYCGRG 1 cut(s) 479
BslI CCNNNNNNNGG 3 cut(s) 13, 164, 322
BsmI GAATGC 1 cut(s) 70
BsnI GGCC 2 cut(s) 5, 249
BsoBI CYCGRG 1 cut(s) 479
Bsp1286I GDGCHC 1 cut(s) 56
Bsp143I GATC 3 cut(s) 118, 288, 369
BspACI CCGC 3 cut(s) 75, 196, 323
BspANI GGCC 2 cut(s) 5, 249
BspCNI CTCAG 2 cut(s) 300, 439
BspHI TCATGA 1 cut(s) 426
BspLI GGNNCC 2 cut(s) 169, 194
BspPI GGATC 1 cut(s) 113
BspT107I GGYRCC 1 cut(s) 167
BsrDI GCAATG 1 cut(s) 191
BsrI ACTGG 1 cut(s) 241
BssECI CCNNGG 5 cut(s) 264, 317, 396, 418, 480
BssMI GATC 3 cut(s) 118, 288, 369
BssT1I CCWWGG 2 cut(s) 317, 418
Bst4CI ACNGT 7 cut(s) 40, 116, 175, 211, 223, 338, 474
BstDEI CTNAG 2 cut(s) 308, 447
BstDSI CCRYGG 2 cut(s) 264, 396
BstF5I GGATG 2 cut(s) 250, 274
BstKTI GATC 3 cut(s) 121, 291, 372
BstMBI GATC 3 cut(s) 118, 288, 369
BstMCI CGRYCG 1 cut(s) 337
BstMWI GCNNNNNNNGC 4 cut(s) 176, 195, 398, 407
BstV1I GCAGC 1 cut(s) 324
BstV2I GAAGAC 1 cut(s) 308
BstX2I RGATCY 1 cut(s) 288
BstYI RGATCY 1 cut(s) 288
BsuRI GGCC 2 cut(s) 5, 249
BtgI CCRYGG 2 cut(s) 264, 396
BtsCI GGATG 2 cut(s) 250, 274
BtsIMutI CAGTG 1 cut(s) 305
CaiI CAGNNNCTG 1 cut(s) 446
CciI TCATGA 1 cut(s) 426
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 2 cut(s) 112, 461
CviAII CATG 1 cut(s) 427
CviJI RGCY 8 cut(s) 5, 26, 179, 195, 249, 315, 359, 401
CviKI_1 RGCY 8 cut(s) 5, 26, 179, 195, 249, 315, 359, 401
CviQI GTAC 2 cut(s) 112, 461
DdeI CTNAG 2 cut(s) 308, 447
DpnI GATC 3 cut(s) 120, 290, 371
DpnII GATC 3 cut(s) 118, 288, 369
EciI GGCGGA 1 cut(s) 338
Eco130I CCWWGG 2 cut(s) 317, 418
Eco88I CYCGRG 1 cut(s) 479
EcoT14I CCWWGG 2 cut(s) 317, 418
ErhI CCWWGG 2 cut(s) 317, 418
FaeI CATG 1 cut(s) 430
FaiI YATR 5 cut(s) 200, 217, 376, 408, 428
FatI CATG 1 cut(s) 426
FbaI TGATCA 1 cut(s) 369
Fnu4HI GCNGC 3 cut(s) 76, 196, 313
FokI GGATG 2 cut(s) 257, 281
Fsp4HI GCNGC 3 cut(s) 76, 196, 313
FspBI CTAG 2 cut(s) 318, 360
GluI GCNGC 3 cut(s) 76, 196, 313
HaeIII GGCC 2 cut(s) 5, 249
Hin1II CATG 1 cut(s) 430
HinfI GANTC 2 cut(s) 18, 147
HphI GGTGA 2 cut(s) 28, 310
Hpy166II GTNNAC 1 cut(s) 112
Hpy188III TCNNGA 3 cut(s) 427, 446, 479
Hpy8I GTNNAC 1 cut(s) 112
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 4 cut(s) 95, 158, 349, 427
HpyCH4III ACNGT 7 cut(s) 40, 116, 175, 211, 223, 338, 474
HpyCH4V TGCA 3 cut(s) 184, 189, 330
HpyF10VI GCNNNNNNNGC 4 cut(s) 176, 195, 398, 407
HpyF3I CTNAG 2 cut(s) 308, 447
Hsp92II CATG 1 cut(s) 430
Ksp22I TGATCA 1 cut(s) 369
Kzo9I GATC 3 cut(s) 118, 288, 369
LmnI GCTCC 2 cut(s) 192, 484
LpnPI CCDG 6 cut(s) 20, 136, 157, 175, 222, 459
Lsp1109I GCAGC 1 cut(s) 324
MaeI CTAG 2 cut(s) 318, 360
MaeIII GTNAC 1 cut(s) 19
MalI GATC 3 cut(s) 120, 290, 371
MboI GATC 3 cut(s) 118, 288, 369
MboII GAAGA 2 cut(s) 313, 442
MflI RGATCY 1 cut(s) 288
MhlI GDGCHC 1 cut(s) 56
MluCI AATT 3 cut(s) 81, 134, 202
MlyI GAGTC 1 cut(s) 27
MnlI CCTC 5 cut(s) 265, 277, 289, 403, 475
MroXI GAANNNNTTC 2 cut(s) 441, 474
MspA1I CMGCKG 1 cut(s) 26
Mva1269I GAATGC 1 cut(s) 70
MwoI GCNNNNNNNGC 4 cut(s) 176, 195, 398, 407
NdeII GATC 3 cut(s) 118, 288, 369
NlaIII CATG 1 cut(s) 430
NlaIV GGNNCC 2 cut(s) 169, 194
NmuCI GTSAC 1 cut(s) 19
PagI TCATGA 1 cut(s) 426
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PctI GAATGC 1 cut(s) 70
PdmI GAANNNNTTC 2 cut(s) 441, 474
PfeI GAWTC 1 cut(s) 147
PflMI CCANNNNNTGG 1 cut(s) 13
PkrI GCNGC 3 cut(s) 77, 197, 314
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PspN4I GGNNCC 2 cut(s) 169, 194
PspPI GGNCC 1 cut(s) 4
PstNI CAGNNNCTG 1 cut(s) 446
PsuI RGATCY 1 cut(s) 288
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 2 cut(s) 113, 462
RsaNI GTAC 2 cut(s) 112, 461
SatI GCNGC 3 cut(s) 76, 196, 313
Sau3AI GATC 3 cut(s) 118, 288, 369
Sau96I GGNCC 1 cut(s) 4
ScaI AGTACT 1 cut(s) 462
SchI GAGTC 1 cut(s) 27
SduI GDGCHC 1 cut(s) 56
SetI ASST 4 cut(s) 28, 111, 169, 300
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
Sse9I AATT 3 cut(s) 81, 134, 202
SsiI CCGC 3 cut(s) 75, 196, 323
SspMI CTAG 2 cut(s) 318, 360
StyI CCWWGG 2 cut(s) 317, 418
TaaI ACNGT 7 cut(s) 40, 116, 175, 211, 223, 338, 474
TaqI TCGA 3 cut(s) 99, 162, 342
TasI AATT 3 cut(s) 81, 134, 202
TatI WGTACW 2 cut(s) 111, 460
TauI GCSGC 2 cut(s) 78, 198
TfiI GAWTC 1 cut(s) 147
TscAI CASTG 1 cut(s) 312
TseFI GTSAC 1 cut(s) 19
TseI GCWGC 1 cut(s) 312
Tsp45I GTSAC 1 cut(s) 19
TspDTI ATGAA 2 cut(s) 415, 443
TspRI CASTG 1 cut(s) 312
Van91I CCANNNNNTGG 1 cut(s) 13
XapI RAATTY 1 cut(s) 202
XmaJI CCTAGG 1 cut(s) 317
XmnI GAANNNNTTC 2 cut(s) 441, 474
XspI CTAG 2 cut(s) 318, 360
ZrmI AGTACT 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.