Rw0G010930

Olee1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00471
OG0016288
Physical Location & Seq
Reverse (-)
21354 .. 21973
620 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G010930.1

Sequence Viewer

Length: 438 bp
ATGGCCCAGACTTTGGGAGTCACAGCTGTTGTTCTCACCGTATTTTGCTTGTGCTCTTTGGCGAATGCCGACGGGCAGAATTACGACCATTTGATGCATGCAGGTGCCACAGTAGCCTTGCAATGCAGCAGCCGCATAAATTCAACCGTCTTATACACAGTTGAACGCAAGACCGGTAAGGATGGCCTTTACACTATTCCCGTAGTTGAGGACTACGCAGAGGAGATCTGCGAGGTGAAGACAGTGAGCAGCCCTAGGGCGGATTGCAACGACCGTTTCGATAAGTTTGAGAAGGCTAGAGTTTTGATCACATACAACAATGGTGTTGCCTCCACAACTCGTTATGCCAATCCCCTTGGGTTCATGAAGAAGGAAGCAGTTCCTGAGTGCGAGAAAGTACTCAAGGAACTGTTTCCCGAGGAGCAGACCGATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

145

Amino Acids

16.05

Weight (kDa)

4.98

Isoelectric Point (pI)

38.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 14 - 92 7e-09 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016288)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g18470
malus_domestica MD03G1241700.v1.1 MD11G1261000.v1.1
prunus_persica Prupe.4G167200_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0031231
rosa_laevigata RLG00000033293
rosa_multiflora Rmu_sc0004014.1_g000008
rosa_roxburghii Rroxscaffold_1G00049050
rosa_rugosa Rorug05G0123000
rosa_samantha Rh5AG216600 Rh5BG216000 Rh5CG239800 Rh5DG221100
rosa_wichuraiana Rw0G010930 Rw5G019810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 92
Acc36I ACCTGC 1 cut(s) 92
AccB1I GGYRCC 1 cut(s) 104
AccB7I CCANNNNNTGG 1 cut(s) 13
AciI CCGC 2 cut(s) 133, 260
AcsI RAATTY 1 cut(s) 139
AfaI GTAC 1 cut(s) 399
AfiI CCNNNNNNNGG 2 cut(s) 13, 259
AgeI ACCGGT 1 cut(s) 173
AgsI TTSAA 2 cut(s) 144, 164
AluBI AGCT 1 cut(s) 26
AluI AGCT 1 cut(s) 26
Alw21I GWGCWC 1 cut(s) 56
AlwNI CAGNNNCTG 1 cut(s) 383
Ama87I CYCGRG 1 cut(s) 416
AoxI GGCC 2 cut(s) 3, 184
ApeKI GCWGC 3 cut(s) 126, 129, 249
ApoI RAATTY 1 cut(s) 139
AsiGI ACCGGT 1 cut(s) 173
Asp700I GAANNNNTTC 2 cut(s) 378, 411
AspA2I CCTAGG 1 cut(s) 254
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 2 cut(s) 28, 247
AvaI CYCGRG 1 cut(s) 416
AvrII CCTAGG 1 cut(s) 254
BanI GGYRCC 1 cut(s) 104
BbsI GAAGAC 1 cut(s) 245
Bbv12I GWGCWC 1 cut(s) 56
BbvI GCAGC 3 cut(s) 138, 141, 261
BccI CCATC 1 cut(s) 176
BclI TGATCA 1 cut(s) 306
BfaI CTAG 2 cut(s) 255, 297
BfuAI ACCTGC 1 cut(s) 92
BglII AGATCT 1 cut(s) 225
BisI GCNGC 4 cut(s) 127, 130, 133, 250
BlnI CCTAGG 1 cut(s) 254
BlsI GCNGC 4 cut(s) 128, 131, 134, 251
BmcAI AGTACT 1 cut(s) 399
BmeT110I CYCGRG 1 cut(s) 416
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 106
BmsI GCATC 1 cut(s) 84
BpiI GAAGAC 1 cut(s) 245
BpuEI CTTGAG 1 cut(s) 386
BsaJI CCNNGG 3 cut(s) 254, 355, 417
BsaWI WCCGGW 1 cut(s) 173
Bsc4I CCNNNNNNNGG 2 cut(s) 13, 259
Bse118I RCCGGY 1 cut(s) 173
Bse3DI GCAATG 1 cut(s) 128
BseDI CCNNGG 3 cut(s) 254, 355, 417
BseGI GGATG 1 cut(s) 187
BseLI CCNNNNNNNGG 2 cut(s) 13, 259
BseMI GCAATG 1 cut(s) 128
BseMII CTCAG 1 cut(s) 375
BseRI GAGGAG 2 cut(s) 236, 434
BseXI GCAGC 3 cut(s) 138, 141, 261
Bsh1285I CGRYCG 1 cut(s) 274
BshFI GGCC 2 cut(s) 5, 186
BshNI GGYRCC 1 cut(s) 104
BshTI ACCGGT 1 cut(s) 173
BsiEI CGRYCG 1 cut(s) 274
BsiHKAI GWGCWC 1 cut(s) 56
BsiHKCI CYCGRG 1 cut(s) 416
BsiSI CCGG 1 cut(s) 174
BslI CCNNNNNNNGG 2 cut(s) 13, 259
BsmI GAATGC 1 cut(s) 70
BsnI GGCC 2 cut(s) 5, 186
BsoBI CYCGRG 1 cut(s) 416
Bsp1286I GDGCHC 1 cut(s) 56
Bsp143I GATC 2 cut(s) 225, 306
BspACI CCGC 2 cut(s) 133, 260
BspANI GGCC 2 cut(s) 5, 186
BspCNI CTCAG 1 cut(s) 376
BspHI TCATGA 1 cut(s) 363
BspLI GGNNCC 1 cut(s) 106
BspMI ACCTGC 1 cut(s) 92
BspT107I GGYRCC 1 cut(s) 104
BsrDI GCAATG 1 cut(s) 128
BsrFI RCCGGY 1 cut(s) 173
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 3 cut(s) 254, 355, 417
BssMI GATC 2 cut(s) 225, 306
BssT1I CCWWGG 2 cut(s) 254, 355
Bst4CI ACNGT 7 cut(s) 40, 112, 148, 160, 244, 275, 411
BstC8I GCNNGC 1 cut(s) 99
BstDEI CTNAG 1 cut(s) 384
BstF5I GGATG 1 cut(s) 187
BstKTI GATC 2 cut(s) 228, 309
BstMBI GATC 2 cut(s) 225, 306
BstMCI CGRYCG 1 cut(s) 274
BstMWI GCNNNNNNNGC 2 cut(s) 113, 132
BstNSI RCATGY 1 cut(s) 101
BstV1I GCAGC 3 cut(s) 138, 141, 261
BstV2I GAAGAC 1 cut(s) 245
BstX2I RGATCY 1 cut(s) 225
BstYI RGATCY 1 cut(s) 225
BsuRI GGCC 2 cut(s) 5, 186
BtsCI GGATG 1 cut(s) 187
BtsIMutI CAGTG 1 cut(s) 249
BveI ACCTGC 1 cut(s) 92
Cac8I GCNNGC 1 cut(s) 99
CaiI CAGNNNCTG 1 cut(s) 383
CciI TCATGA 1 cut(s) 363
Cfr10I RCCGGY 1 cut(s) 173
Cfr13I GGNCC 1 cut(s) 4
Csp6I GTAC 1 cut(s) 398
CspAI ACCGGT 1 cut(s) 173
CviAII CATG 2 cut(s) 98, 364
CviJI RGCY 7 cut(s) 5, 26, 116, 132, 186, 252, 296
CviKI_1 RGCY 7 cut(s) 5, 26, 116, 132, 186, 252, 296
CviQI GTAC 1 cut(s) 398
DdeI CTNAG 1 cut(s) 384
DpnI GATC 2 cut(s) 227, 308
DpnII GATC 2 cut(s) 225, 306
EciI GGCGGA 1 cut(s) 275
Eco130I CCWWGG 2 cut(s) 254, 355
Eco88I CYCGRG 1 cut(s) 416
EcoT14I CCWWGG 2 cut(s) 254, 355
EcoT22I ATGCAT 1 cut(s) 99
ErhI CCWWGG 2 cut(s) 254, 355
FaeI CATG 2 cut(s) 101, 367
FaiI YATR 6 cut(s) 99, 137, 154, 313, 345, 365
FatI CATG 2 cut(s) 97, 363
FbaI TGATCA 1 cut(s) 306
Fnu4HI GCNGC 4 cut(s) 127, 130, 133, 250
FokI GGATG 1 cut(s) 194
Fsp4HI GCNGC 4 cut(s) 127, 130, 133, 250
FspBI CTAG 2 cut(s) 255, 297
GluI GCNGC 4 cut(s) 127, 130, 133, 250
HaeIII GGCC 2 cut(s) 5, 186
HapII CCGG 1 cut(s) 174
Hin1II CATG 2 cut(s) 101, 367
HinfI GANTC 1 cut(s) 18
HpaII CCGG 1 cut(s) 174
HphI GGTGA 2 cut(s) 28, 247
Hpy188III TCNNGA 3 cut(s) 364, 383, 416
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 2 cut(s) 286, 364
HpyCH4III ACNGT 7 cut(s) 40, 112, 148, 160, 244, 275, 411
HpyCH4V TGCA 5 cut(s) 97, 101, 121, 126, 267
HpyF10VI GCNNNNNNNGC 2 cut(s) 113, 132
HpyF3I CTNAG 1 cut(s) 384
Hsp92II CATG 2 cut(s) 101, 367
Ksp22I TGATCA 1 cut(s) 306
Kzo9I GATC 2 cut(s) 225, 306
LmnI GCTCC 1 cut(s) 421
LpnPI CCDG 4 cut(s) 20, 87, 187, 396
Lsp1109I GCAGC 3 cut(s) 138, 141, 261
LweI GCATC 1 cut(s) 84
MaeI CTAG 2 cut(s) 255, 297
MaeIII GTNAC 1 cut(s) 19
MalI GATC 2 cut(s) 227, 308
MboI GATC 2 cut(s) 225, 306
MboII GAAGA 2 cut(s) 250, 379
MflI RGATCY 1 cut(s) 225
MhlI GDGCHC 1 cut(s) 56
MluCI AATT 2 cut(s) 79, 139
MlyI GAGTC 1 cut(s) 27
MnlI CCTC 5 cut(s) 202, 214, 226, 340, 412
Mph1103I ATGCAT 1 cut(s) 99
MroXI GAANNNNTTC 2 cut(s) 378, 411
MslI CAYNNNNRTG 1 cut(s) 102
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 1 cut(s) 174
Mva1269I GAATGC 1 cut(s) 70
MwoI GCNNNNNNNGC 2 cut(s) 113, 132
NdeII GATC 2 cut(s) 225, 306
NlaIII CATG 2 cut(s) 101, 367
NlaIV GGNNCC 1 cut(s) 106
NmuCI GTSAC 1 cut(s) 19
NsiI ATGCAT 1 cut(s) 99
NspI RCATGY 1 cut(s) 101
PaeI GCATGC 1 cut(s) 101
PagI TCATGA 1 cut(s) 363
PaqCI CACCTGC 1 cut(s) 92
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PctI GAATGC 1 cut(s) 70
PdmI GAANNNNTTC 2 cut(s) 378, 411
PflMI CCANNNNNTGG 1 cut(s) 13
PinAI ACCGGT 1 cut(s) 173
PkrI GCNGC 4 cut(s) 128, 131, 134, 251
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
PspN4I GGNNCC 1 cut(s) 106
PspPI GGNCC 1 cut(s) 4
PstNI CAGNNNCTG 1 cut(s) 383
PsuI RGATCY 1 cut(s) 225
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 1 cut(s) 399
RsaNI GTAC 1 cut(s) 398
RseI CAYNNNNRTG 1 cut(s) 102
SatI GCNGC 4 cut(s) 127, 130, 133, 250
Sau3AI GATC 2 cut(s) 225, 306
Sau96I GGNCC 1 cut(s) 4
ScaI AGTACT 1 cut(s) 399
SchI GAGTC 1 cut(s) 27
SduI GDGCHC 1 cut(s) 56
SetI ASST 3 cut(s) 28, 106, 237
SfaNI GCATC 1 cut(s) 84
SmiMI CAYNNNNRTG 1 cut(s) 102
SmlI CTYRAG 1 cut(s) 401
SmoI CTYRAG 1 cut(s) 401
SphI GCATGC 1 cut(s) 101
Sse9I AATT 2 cut(s) 79, 139
SsiI CCGC 2 cut(s) 133, 260
SspMI CTAG 2 cut(s) 255, 297
StyI CCWWGG 2 cut(s) 254, 355
TaaI ACNGT 7 cut(s) 40, 112, 148, 160, 244, 275, 411
TaqI TCGA 1 cut(s) 279
TasI AATT 2 cut(s) 79, 139
TatI WGTACW 1 cut(s) 397
TauI GCSGC 1 cut(s) 135
TscAI CASTG 1 cut(s) 249
TseFI GTSAC 1 cut(s) 19
TseI GCWGC 3 cut(s) 126, 129, 249
Tsp45I GTSAC 1 cut(s) 19
TspDTI ATGAA 2 cut(s) 352, 380
TspRI CASTG 1 cut(s) 249
Van91I CCANNNNNTGG 1 cut(s) 13
XapI RAATTY 1 cut(s) 139
XceI RCATGY 1 cut(s) 101
XmaJI CCTAGG 1 cut(s) 254
XmnI GAANNNNTTC 2 cut(s) 378, 411
XspI CTAG 2 cut(s) 255, 297
ZrmI AGTACT 1 cut(s) 399
Zsp2I ATGCAT 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.