RLG00000033298

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0019020
Physical Location & Seq
Forward (+)
25427540 .. 25429461
1922 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033298

Sequence Viewer

Length: 585 bp
ATGGGAGGTCGGCAGAGGCGGACTTCGTGCGTGCAACCGGTAGCGTGCATGGTGGCTTCGAGTAAAGCTTGGACGGTAGGTGGCGACCTTTCGAGACGGTCAGAAAAAGTCAGGCGGCGCAACGGTGGGGCTGGTCGACGGGCTGGACGTCGGCGAGGACTGGAGATGGGACGGCGTCACCGTAAGGTTAGTGACGGAGGGTTACCCGCTACTCGACGCATTTCGATGTATGGAGATGGTCAGAGTGATTTAGTTGTTAATGTTGGAGAATCTACATCTTCAACCAATGTTCCTTCGCATGCCATGGATATGGATGTGAGTGCACCATTTACATTGGTTGATGAGATAGCTGGTGCATCACCAGCAACGGAGGGTGGCATACAACTAGGAGATCAGATTGTGAAATTTGGCAATGTGGAAAATGGTGACAATTTGTTGCGGAAACTTGCTTCCGAGGCTCAAGCAAATCTGTGTTGTGCTGTGCTTGTTATGGTCTTAAGGCAAGGTGCCCAATTCAACTTAATGGTGACACGTAGAACATGGCAAGGCAGAGGTCTACTGGGGTGTCATTTCCAGATTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDZ_2

Protein Analysis

195

Amino Acids

20.91

Weight (kDa)

10.7

Isoelectric Point (pI)

68.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019020)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0031291
rosa_laevigata RLG00000019651 RLG00000033298
rosa_rugosa Rorug05G0123800
rosa_samantha Rh5AG217100 Rh5BG216800 Rh5CG240400 Rh5DG221900
rosa_wichuraiana Rw5G019850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 151
AccB1I GGYRCC 1 cut(s) 506
AccI GTMKAC 2 cut(s) 136, 556
AciI CCGC 4 cut(s) 19, 115, 207, 439
AcsI RAATTY 1 cut(s) 404
AcyI GRCGYC 2 cut(s) 148, 175
AflII CTTAAG 1 cut(s) 496
AflIII ACRYGT 1 cut(s) 530
AgeI ACCGGT 1 cut(s) 37
AgsI TTSAA 2 cut(s) 282, 517
AluBI AGCT 2 cut(s) 68, 350
AluI AGCT 2 cut(s) 68, 350
Alw21I GWGCWC 1 cut(s) 325
Alw26I GTCTC 1 cut(s) 88
Alw44I GTGCAC 1 cut(s) 321
ApaLI GTGCAC 1 cut(s) 321
ApoI RAATTY 1 cut(s) 404
AsiGI ACCGGT 1 cut(s) 37
AspLEI GCGC 1 cut(s) 120
AsuHPI GGTGA 4 cut(s) 170, 351, 437, 538
BaeGI GKGCMC 2 cut(s) 325, 511
BanI GGYRCC 1 cut(s) 506
Bbv12I GWGCWC 1 cut(s) 325
BccI CCATC 2 cut(s) 160, 230
BceAI ACGGC 1 cut(s) 188
BcoDI GTCTC 1 cut(s) 88
BfaI CTAG 1 cut(s) 386
BfrI CTTAAG 1 cut(s) 496
BisI GCNGC 1 cut(s) 116
BlsI GCNGC 1 cut(s) 117
BmiI GGNNCC 1 cut(s) 508
BmrI ACTGGG 1 cut(s) 569
BmsI GCATC 1 cut(s) 365
BmuI ACTGGG 1 cut(s) 569
BpmI CTGGAG 1 cut(s) 182
BpuEI CTTGAG 1 cut(s) 444
BsaAI YACGTR 1 cut(s) 533
BsaHI GRCGYC 2 cut(s) 148, 175
BsaJI CCNNGG 2 cut(s) 303, 453
BsaWI WCCGGW 1 cut(s) 37
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 2 cut(s) 165, 564
Bse3DI GCAATG 1 cut(s) 418
BseDI CCNNGG 2 cut(s) 303, 453
BseGI GGATG 1 cut(s) 319
BseMI GCAATG 1 cut(s) 418
BseNI ACTGG 2 cut(s) 165, 564
BseSI GKGCMC 2 cut(s) 325, 511
BshNI GGYRCC 1 cut(s) 506
BshTI ACCGGT 1 cut(s) 37
BsiHKAI GWGCWC 1 cut(s) 325
BsiSI CCGG 1 cut(s) 38
BslFI GGGAC 1 cut(s) 183
BsmAI GTCTC 1 cut(s) 88
BsmBI CGTCTC 1 cut(s) 88
BsmFI GGGAC 1 cut(s) 183
Bsp1286I GDGCHC 2 cut(s) 325, 511
Bsp143I GATC 1 cut(s) 391
Bsp19I CCATGG 1 cut(s) 303
BspACI CCGC 4 cut(s) 19, 115, 207, 439
BspLI GGNNCC 1 cut(s) 508
BspT107I GGYRCC 1 cut(s) 506
BspTI CTTAAG 1 cut(s) 496
BsrDI GCAATG 1 cut(s) 418
BsrFI RCCGGY 1 cut(s) 37
BsrI ACTGG 2 cut(s) 165, 564
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 2 cut(s) 303, 453
BssMI GATC 1 cut(s) 391
BssNI GRCGYC 2 cut(s) 148, 175
BssT1I CCWWGG 1 cut(s) 303
Bst4CI ACNGT 4 cut(s) 76, 99, 125, 182
BstACI GRCGYC 2 cut(s) 148, 175
BstAFI CTTAAG 1 cut(s) 496
BstBAI YACGTR 1 cut(s) 533
BstC8I GCNNGC 3 cut(s) 32, 46, 300
BstDSI CCRYGG 1 cut(s) 303
BstEII GGTNACC 1 cut(s) 201
BstF5I GGATG 1 cut(s) 319
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 1 cut(s) 394
BstMAI GTCTC 1 cut(s) 88
BstMBI GATC 1 cut(s) 391
BstMWI GCNNNNNNNGC 2 cut(s) 362, 455
BstNSI RCATGY 1 cut(s) 302
BstPI GGTNACC 1 cut(s) 201
BstSLI GKGCMC 2 cut(s) 325, 511
BstXI CCANNNNNNTGG 1 cut(s) 310
BtgI CCRYGG 1 cut(s) 303
BtsCI GGATG 1 cut(s) 319
Cac8I GCNNGC 3 cut(s) 32, 46, 300
CfoI GCGC 1 cut(s) 120
Cfr10I RCCGGY 1 cut(s) 37
CseI GACGC 2 cut(s) 164, 225
CspAI ACCGGT 1 cut(s) 37
CviAII CATG 4 cut(s) 49, 299, 304, 540
CviJI RGCY 6 cut(s) 56, 68, 131, 143, 350, 458
CviKI_1 RGCY 6 cut(s) 56, 68, 131, 143, 350, 458
DpnI GATC 1 cut(s) 393
DpnII GATC 1 cut(s) 391
EciI GGCGGA 1 cut(s) 34
Eco130I CCWWGG 1 cut(s) 303
Eco91I GGTNACC 1 cut(s) 201
EcoO65I GGTNACC 1 cut(s) 201
EcoT14I CCWWGG 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 303
Esp3I CGTCTC 1 cut(s) 88
FaeI CATG 4 cut(s) 52, 302, 307, 543
FaiI YATR 8 cut(s) 50, 231, 300, 305, 311, 380, 491, 541
FaqI GGGAC 1 cut(s) 183
FatI CATG 4 cut(s) 48, 298, 303, 539
FauI CCCGC 1 cut(s) 214
FblI GTMKAC 2 cut(s) 136, 556
Fnu4HI GCNGC 1 cut(s) 116
FokI GGATG 1 cut(s) 326
Fsp4HI GCNGC 1 cut(s) 116
FspBI CTAG 1 cut(s) 386
GlaI GCGC 1 cut(s) 119
GluI GCNGC 1 cut(s) 116
GsuI CTGGAG 1 cut(s) 182
HapII CCGG 1 cut(s) 38
HgaI GACGC 2 cut(s) 164, 225
HhaI GCGC 1 cut(s) 120
Hin1I GRCGYC 2 cut(s) 148, 175
Hin1II CATG 4 cut(s) 52, 302, 307, 543
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HincII GTYRAC 1 cut(s) 137
HindII GTYRAC 1 cut(s) 137
HindIII AAGCTT 1 cut(s) 66
HinfI GANTC 1 cut(s) 269
HpaII CCGG 1 cut(s) 38
HphI GGTGA 4 cut(s) 170, 351, 437, 538
Hpy166II GTNNAC 3 cut(s) 137, 323, 557
Hpy188I TCNGA 4 cut(s) 103, 243, 396, 454
Hpy188III TCNNGA 2 cut(s) 93, 574
Hpy8I GTNNAC 3 cut(s) 137, 323, 557
Hpy99I CGWCG 3 cut(s) 141, 153, 219
HpyAV CCTTC 1 cut(s) 303
HpyCH4III ACNGT 4 cut(s) 76, 99, 125, 182
HpyCH4IV ACGT 2 cut(s) 148, 532
HpyCH4V TGCA 4 cut(s) 34, 48, 323, 356
HpyF10VI GCNNNNNNNGC 2 cut(s) 362, 455
HpySE526I ACGT 2 cut(s) 148, 532
Hsp92I GRCGYC 2 cut(s) 148, 175
Hsp92II CATG 4 cut(s) 52, 302, 307, 543
HspAI GCGC 1 cut(s) 118
Kzo9I GATC 1 cut(s) 391
LpnPI CCDG 8 cut(s) 51, 97, 117, 129, 146, 336, 375, 545
LweI GCATC 1 cut(s) 365
MaeI CTAG 1 cut(s) 386
MaeII ACGT 2 cut(s) 148, 532
MaeIII GTNAC 5 cut(s) 176, 191, 201, 425, 526
MalI GATC 1 cut(s) 393
MboI GATC 1 cut(s) 391
MboII GAAGA 1 cut(s) 270
MhlI GDGCHC 2 cut(s) 325, 511
MluCI AATT 3 cut(s) 404, 430, 512
MmeI TCCRAC 1 cut(s) 244
MnlI CCTC 6 cut(s) 9, 149, 191, 364, 448, 545
MseI TTAA 3 cut(s) 258, 497, 521
MslI CAYNNNNRTG 2 cut(s) 224, 308
MspCI CTTAAG 1 cut(s) 496
MspI CCGG 1 cut(s) 38
MwoI GCNNNNNNNGC 2 cut(s) 362, 455
NcoI CCATGG 1 cut(s) 303
NdeII GATC 1 cut(s) 391
NlaIII CATG 4 cut(s) 52, 302, 307, 543
NlaIV GGNNCC 1 cut(s) 508
NmuCI GTSAC 4 cut(s) 176, 191, 425, 526
NspI RCATGY 1 cut(s) 302
PaeI GCATGC 1 cut(s) 302
PcsI WCGNNNNNNNCGW 2 cut(s) 145, 178
PfeI GAWTC 1 cut(s) 269
PflFI GACNNNGTC 1 cut(s) 174
PinAI ACCGGT 1 cut(s) 37
PkrI GCNGC 1 cut(s) 117
Ppu21I YACGTR 1 cut(s) 533
PspEI GGTNACC 1 cut(s) 201
PspN4I GGNNCC 1 cut(s) 508
PsyI GACNNNGTC 1 cut(s) 174
RseI CAYNNNNRTG 2 cut(s) 224, 308
SalI GTCGAC 1 cut(s) 135
SaqAI TTAA 3 cut(s) 258, 497, 521
SatI GCNGC 1 cut(s) 116
Sau3AI GATC 1 cut(s) 391
SduI GDGCHC 2 cut(s) 325, 511
SfaNI GCATC 1 cut(s) 365
SmiMI CAYNNNNRTG 2 cut(s) 224, 308
SmlI CTYRAG 2 cut(s) 459, 496
SmoI CTYRAG 2 cut(s) 459, 496
SphI GCATGC 1 cut(s) 302
Sse9I AATT 3 cut(s) 404, 430, 512
SsiI CCGC 4 cut(s) 19, 115, 207, 439
SspMI CTAG 1 cut(s) 386
StyI CCWWGG 1 cut(s) 303
TaaI ACNGT 4 cut(s) 76, 99, 125, 182
TaiI ACGT 2 cut(s) 151, 535
TaqI TCGA 5 cut(s) 59, 92, 136, 214, 224
TasI AATT 3 cut(s) 404, 430, 512
TauI GCSGC 1 cut(s) 118
TfiI GAWTC 1 cut(s) 269
Tru1I TTAA 3 cut(s) 258, 497, 521
Tru9I TTAA 3 cut(s) 258, 497, 521
TseFI GTSAC 4 cut(s) 176, 191, 425, 526
Tsp45I GTSAC 4 cut(s) 176, 191, 425, 526
TspGWI ACGGA 2 cut(s) 210, 383
Tth111I GACNNNGTC 1 cut(s) 174
Vha464I CTTAAG 1 cut(s) 496
VneI GTGCAC 1 cut(s) 321
XapI RAATTY 1 cut(s) 404
XceI RCATGY 1 cut(s) 302
XmiI GTMKAC 2 cut(s) 136, 556
XspI CTAG 1 cut(s) 386
ZraI GACGTC 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.