Rorug05G0123800

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0019020
Physical Location & Seq
Forward (+)
10922060 .. 10928022
5963 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0123800.1

Sequence Viewer

Length: 3654 bp
ATGCTGACGAAATTCGAGACCAAGAGTAACCGTGTCAAGGGGCTGAGCTTCCACAGCAAGAGGCCATGGATCTTGGCGAGTCTTCACAGCGGTGTGATCCAGCTCTGGGATTACCGCATGGGCACTCTCATCGACCGCTTTGACGAGCACGATGGCCCCGTCCGCGGCGTCCATTTCCACACCTCTCAGCCTCTTTTCGTCTCCGGCGGGGATGATTACAAGATCAAGGTGTGGAATTATAAGTTGCATAGGTGCCTGTTTACTCTTCTTGGACACCTTGATTACATCCGTACTGTTCAATTCCATCATGAGTACCCTTGGATTGTGAGTGCCAGTGATGACCAGACTATCAGAATATGGAATTGGCAGTCCCGGACTTGTATTTCTGTGCTCACTGGACACAACCATTACGTCATGTGTGCCTTGTTCCATCCCAAGGAGGATCTTGTTGTCTCAGCATCATTGGATCAAACTGTTCGTGTTTGGGATATTGGTGCACTCAGGAAGAAAACAGTTGCGCCTGCAGATGACATCCTACGACTGAGTCAAATGAATGCAGATTTCTTTGGTGGAGTTGATGCTGTGGTTAAGTATGTTTTGGAAGGCCATGATCGGGGTGTTAATTGGGCCTCCTTCCATCCTACACTCCCTCTGATTGTGTCTGGAGCTGATGATCGACAAGTGAAACTTTGGCGAATGAATGATACAAAGGCTTGGGAGGTGGACACTTTAAGGGGGCACATGAACAATGTATCATGTGTTTTATTCCATGCGAGGCAGGATATTATTGTTTCCAATTCAGAGGATAGGAGTATCCGAGTCTGGGATGCCACAAAAAGAACTGGTCTTCAGACATTTCGCAGAGAGCATGATAGGTTCTGGATTCTATCAGCACACCCTGAAATGAACCTTTTGGCTGCTGGTCATGATAGTGGCATGATTGTCTTTAAATTGGAGAGAGAACGCCCTGCCTTTTCTGTCAGTGCTGATTCTATGTACTATGTCAAAGATCGTTTTCTCCGCTTTTATGAGTTCTCAAACCAGAGAGACACTCAGGTTATCCCTATACGAAGGCCCGGTTCTTCCACCTTGAATCAAGGGGCTAAAACTCTATCTTATAGCCCTACAGAAAATGCTGTTTTGATCTGTTCAGATACGGAAGGCGGTTCCTATGAACTATACATAATTCCCAAAGAAAGCTATGGTCGGGGTGATACTGTGCAGGAGGCAAAAAGAGGAGCTGGAGGGCCAGCGGTTTTTGTGGCTCGAAATAGATTTGCTGTGCTTGAAAAAAGCACAAACCAAGTCTTAGTTAAAAATCTGAAAAATGAGATTATCAAGAAGAGCGCCCTTCCTTTTGTTGCTGATGCAATATATTATGCTGGAACTGGAAACTTGCTCTGCAGGGCAGAGGATAGAGTTGTCATTTTTGACCTGCAGCAGAGAATTATTCTTGGGGAGCTTCAAACTCCGTTTGTAAGGTACATAGTTTGGTCAAATGACATGGAGAACATTGCTTTACTCAGCAAACATTCCATTGTGATAGCCAATAAGAAGCTCGTTCATCAATGTACCCTACATGAGACCATTCGTGTAAAGAGTGGAGCTTGGGATGACCATGGTGTCTTCATATATACAACTCTGAACCATATCAAATACTGTCTTCCTAATGGGGACAGTGGAATCATTAGGACCCTTGATGTTCCCGTTTATATAACAAAGGTGTATGGGAGCACCATACATTGCTTGGATCGAGATGGGAAGAACTGTGCAATTGTTGTTGATGCAACAGAATATGTTTTTAAGTTGTCCTTACTGAAGAAGAGGTACGACCAGGTTATGAGCATGATTAGGAGCTCTGAGCTCTGTGGCCAGGCCATGATTGCATATCTACAGCAGAAAGGTTTCCCTGAGGTTGCCCTTCATTTTGTGAAGGATGAGAGGACTCGCTTCAACTTGGCACTAGGGAGTGGAAACATACAGATTGCTGTTGCTTCTGCTAAGGAAATTGATGAGAAAGATCACTGGTATCGGTTGGGAGTTGAGGCTCTTCGTCAAGGTAATTCAGGTATTGTAGAATATGCATATCAGAGGACAAAGAATTTTGAGAGGCTATCATTCCTATATCTTGTTACTGGAAACTTGGATAAGTTATCCAAAATGCTGAAAATCGCTGAAGTGAAGAATGATGTGATGGGCCAGTTCCACAATGCTCTATATTTGGGTGACATCGGGGAGCGTGTTAAGATATTGGAGAATGCTGGGCATTTACCCCTTGCATATGCTACAGCTAAAGCTCATGGCCTCCAAGATATTGCTGAACGGTTGGCAGGTGAGCTGGGAGATAATGTTCCTATTTTACCTGAAGGAAAGTCACCATCTCTTCTGGTACCTCCTAGCCCAATTATCTGTGGTGGAGATTGGCCCTTGTTGAGGGTCATGAGAGGAATATTTGAGGGTGGTCTTGATAATGTGGGCAGAAATGCTGAGGAAGAATATGAGGAGGCTGCAGATGCTGATTGGGGAGAGGATTTGGATATTGTTGATGTGGAAAACATTCAGAACGGAGACATTACTGCAGTGCTGGAAGATGAGGACACACATGAGGAAAATGAAGAGGGAGGGTGGGATCTTGAAGATCTTGAACTTCCACTGGAGATTGATACTCCCAAAACTGCCAACAATGCCCGTGCTTCCGTGTTTGTTGCCCCAACTCCAGGTATGCCTGTAAGTCAAATTTGGAGCCAAAAATCTTCTCTTGCTGCTGAACATGCAGCAGCTGGGAATTTTGATATTGCAATGCGATTATTGAGCCGGCAGCTGGGAATAAAGAACTTTGCACCCTTAAAACAATTGTTTCTTGACTTGCACACGGGGAGTCATTCGTATTTACGCGCCTTATCGACAGCTCCTGTGATCTCCTTGGCTATTGAGAGGGGATGGAGTGAATCAGTAAGCCCTAATGTTAGGAGCCCTCCTGCTTTGGTTTTTAAATTCTCAGACCTGGAAGAGAAGCTTAAGGCGGGCTACAAGGCCACAACTACAGGGAAATTTACTGAGGCTTTACGACTTCTACTCGGTATTCTGCACACTATTCCTCTGGTCGTAGTTGATACAAGGAGAGAGGTTGATGAAGTAAAGGAATTAATTATCATTGTAAGGGAATATGTTCTGGGTTTGAAGATGGAGCTTAAGAGGAGGGAGGTTAAAGATAATCCAGTTCGTCAACAGGAGCTAGCAGCTTACTTCACCCACTGTAACCTCCAGATGCCTCACTTGAGACTAGCATTACTGAACGCCATGACTGTGTGCTACAAGGCTGGTAATCTCAACACAGCTGGTAACTTTGCCAGGCGACTATTGGAGACCAATCCCACAAATGAAAACCAAGCAAAGACTGCAAGGCAAGTCCTGCAGGCTGCTGAGAAGAACATGAATGATGCAACCCAGCTCAATTATGATTTCAGAAATCCATTTGTGGTTTGTGGGGCAACATATGTGCCTATTTACCGAGGACAGAAGGATGTGTCATGCCCATACTGTAGTTCTCGGTTTGTTCCTGCTCAGGAAGGCCAGCTCTGTACTGTTTGCGATCTTGCAGTTGTTGGATCTGATGCATCTGGGTTACTCTGCTCTCCGTCCCAGATAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDZ_2

Protein Analysis

1217

Amino Acids

136.86

Weight (kDa)

6.51

Isoelectric Point (pI)

33.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_1st PF25171 2 - 79 1.1e-08 WDR36/Utp21 first beta-propeller
Beta-prop_WDR5 PF25175 3 - 90 5.6e-10 WDR5 beta-propeller domain
WDR55 PF24796 3 - 166 4.4e-12 WDR55
Beta-prop_WDR3_1st PF25173 4 - 164 1.6e-31 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 4 - 162 3.4e-15 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 6 - 104 1.6e-10 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 17 - 83 9.5e-06 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 26 - 122 3.2e-07 G protein beta WD-40 repeat protein
WD40 PF00400 42 - 79 7.1e-06 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 49 - 167 8.2e-08 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 49 - 165 6.6e-16 WDR36/Utp21 second beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 49 - 166 6.4e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR5 PF25175 49 - 166 1.8e-30 WDR5 beta-propeller domain
WD40_Prp19 PF24814 60 - 167 6.7e-20 Prp19 WD40 domain
WD40 PF00400 84 - 121 3e-08 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 88 - 163 1.6e-08 WDR3 second beta-propeller domain
Beta-prop_EIPR1 PF23609 91 - 162 1.9e-06 EIPR1 beta-propeller
WD40_WDHD1_1st PF24817 96 - 166 3.5e-12 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 108 - 167 2.2e-12 THOC3 beta-propeller domain
WD40 PF00400 126 - 163 6.4e-09 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 191 - 306 3.3e-06 CAF1B/HIR1 beta-propeller domain
WD40_Prp19 PF24814 195 - 314 2e-17 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 196 - 317 4.1e-18 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 196 - 308 1.9e-20 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 197 - 292 5.9e-07 TEP-1 second beta-propeller
WD40 PF00400 198 - 231 3e-06 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 202 - 314 6.6e-09 WDR3 second beta-propeller domain
WDR55 PF24796 207 - 315 9.8e-09 WDR55
Beta-prop_THOC3 PF25174 208 - 316 3.3e-14 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 211 - 287 7e-09 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 212 - 319 2.5e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 223 - 321 8.9e-07 CDC20/Fizzy WD40 domain
WD40 PF00400 241 - 276 1.7e-06 WD domain, G-beta repeat
B-prop_COPA_B_2nd PF04053 340 - 584 2.5e-64 COPA/B second beta-propeller
TPR_COPA_B PF23953 626 - 771 2.2e-18 COPA/B TPR domain
COPI_C PF06957 815 - 1217 1.8e-171 Coatomer (COPI) alpha subunit C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019020)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0031291
rosa_laevigata RLG00000019651 RLG00000033298
rosa_rugosa Rorug05G0123800
rosa_samantha Rh5AG217100 Rh5BG216800 Rh5CG240400 Rh5DG221900
rosa_wichuraiana Rw5G019850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 240
AarI CACCTGC 1 cut(s) 2321
AasI GACNNNNNNGTC 1 cut(s) 1622
Acc36I ACCTGC 2 cut(s) 1443, 2321
Acc65I GGTACC 1 cut(s) 2389
AccB1I GGYRCC 2 cut(s) 252, 2389
AccII CGCG 2 cut(s) 165, 2895
AclWI GGATC 7 cut(s) 77, 91, 450, 474, 1758, 2637, 3616
AcoI YGGCCR 1 cut(s) 1870
AcsI RAATTY 6 cut(s) 11, 2101, 2736, 2785, 2993, 3050
AcuI CTGAAG 4 cut(s) 833, 1838, 2196, 2385
AcyI GRCGYC 1 cut(s) 168
AfaI GTAC 8 cut(s) 292, 314, 998, 1484, 1573, 1829, 2391, 3583
AfiI CCNNNNNNNGG 9 cut(s) 37, 106, 164, 436, 613, 823, 2433, 2717, 2821
AflII CTTAAG 2 cut(s) 3017, 3191
AgsI TTSAA 8 cut(s) 299, 1093, 1289, 1466, 1954, 2636, 2645, 3181
AjnI CCWGG 5 cut(s) 1833, 1872, 2716, 3003, 3350
AjuI GAANNNNNNNTTGG 2 cut(s) 346, 378
AleI CACNNNNGTG 1 cut(s) 90
AloI GAACNNNNNNTCC 2 cut(s) 2334, 2366
Alw21I GWGCWC 6 cut(s) 150, 393, 499, 1736, 1859, 1866
Alw26I GTCTC 8 cut(s) 11, 205, 457, 1041, 1577, 2562, 3274, 3359
Alw44I GTGCAC 1 cut(s) 495
AlwI GGATC 7 cut(s) 77, 91, 450, 474, 1758, 2637, 3616
AlwNI CAGNNNCTG 3 cut(s) 2516, 2780, 2912
ApaLI GTGCAC 1 cut(s) 495
ApeKI GCWGC 9 cut(s) 917, 1438, 2507, 2762, 2774, 2777, 2818, 3239, 3419
ApoI RAATTY 6 cut(s) 11, 2101, 2736, 2785, 2993, 3050
AseI ATTAAT 1 cut(s) 3146
Asp700I GAANNNNTTC 3 cut(s) 1904, 2556, 3168
Asp718I GGTACC 1 cut(s) 2389
AspLEI GCGC 3 cut(s) 520, 1349, 2897
AspS9I GGNCC 7 cut(s) 155, 627, 1074, 1247, 1692, 2197, 2424
AsuC2I CCSGG 2 cut(s) 373, 1077
AsuHPI GGTGA 5 cut(s) 1223, 2237, 2345, 2367, 3241
AsuNHI GCTAGC 1 cut(s) 3235
AvaII GGWCC 1 cut(s) 1692
AxyI CCTNAGG 1 cut(s) 1911
BaeGI GKGCMC 3 cut(s) 125, 499, 741
BalI TGGCCA 1 cut(s) 1872
BanI GGYRCC 2 cut(s) 252, 2389
BanII GRGCYC 3 cut(s) 1859, 1866, 2975
BarI GAAGNNNNNNTAC 2 cut(s) 1811, 1843
BbsI GAAGAC 4 cut(s) 74, 839, 1618, 1655
Bbv12I GWGCWC 6 cut(s) 150, 393, 499, 1736, 1859, 1866
BbvCI CCTCAGC 1 cut(s) 2487
BbvI GCAGC 9 cut(s) 904, 1450, 2494, 2749, 2786, 2789, 2830, 3251, 3406
BccI CCATC 9 cut(s) 146, 312, 438, 645, 1751, 2188, 2386, 2934, 3178
BcgI CGANNNNNNTGC 2 cut(s) 112, 146
BciT130I CCWGG 5 cut(s) 1835, 1874, 2718, 3005, 3352
BciVI GTATCC 1 cut(s) 824
BcnI CCSGG 2 cut(s) 373, 1077
BcoDI GTCTC 8 cut(s) 11, 205, 457, 1041, 1577, 2562, 3274, 3359
BfaI CTAG 4 cut(s) 1964, 2397, 3236, 3284
BfoI RGCGCY 1 cut(s) 1350
BfrI CTTAAG 2 cut(s) 3017, 3191
BfuAI ACCTGC 2 cut(s) 1443, 2321
BfuI GTATCC 1 cut(s) 824
BglII AGATCT 1 cut(s) 2638
BlpI GCTNAGC 1 cut(s) 44
Bme1390I CCNGG 7 cut(s) 373, 1077, 1835, 1874, 2718, 3005, 3352
Bme18I GGWCC 1 cut(s) 1692
BmgT120I GGNCC 7 cut(s) 155, 627, 1074, 1247, 1692, 2197, 2424
BmiI GGNNCC 7 cut(s) 157, 254, 1168, 1694, 2391, 2744, 2972
BmrFI CCNGG 7 cut(s) 373, 1077, 1835, 1874, 2718, 3005, 3352
BmtI GCTAGC 1 cut(s) 3239
BpiI GAAGAC 4 cut(s) 74, 839, 1618, 1655
BplI GAGNNNNNCTC 2 cut(s) 1036, 1068
BpmI CTGGAG 5 cut(s) 684, 1263, 2675, 2700, 3248
Bpu10I CCTNAGC 3 cut(s) 2001, 2487, 3564
Bpu1102I GCTNAGC 1 cut(s) 44
BpuEI CTTGAG 1 cut(s) 3298
BpuMI CCSGG 2 cut(s) 373, 1077
BsaHI GRCGYC 1 cut(s) 168
BsaI GGTCTC 3 cut(s) 11, 1577, 3359
BsaJI CCNNGG 7 cut(s) 65, 163, 317, 435, 1618, 2922, 3511
BsaXI ACNNNNNCTCC 2 cut(s) 2334, 2364
Bsc4I CCNNNNNNNGG 9 cut(s) 37, 106, 164, 436, 613, 823, 2433, 2717, 2821
Bse118I RCCGGY 1 cut(s) 2814
Bse1I ACTGG 9 cut(s) 333, 400, 847, 1393, 2030, 2140, 2200, 2658, 3218
Bse21I CCTNAGG 1 cut(s) 1911
Bse3DI GCAATG 3 cut(s) 1512, 1741, 2805
BseBI CCWGG 5 cut(s) 1835, 1874, 2718, 3005, 3352
BseDI CCNNGG 7 cut(s) 65, 163, 317, 435, 1618, 2922, 3511
BseLI CCNNNNNNNGG 9 cut(s) 37, 106, 164, 436, 613, 823, 2433, 2717, 2821
BseMI GCAATG 3 cut(s) 1512, 1741, 2805
BseNI ACTGG 9 cut(s) 333, 400, 847, 1393, 2030, 2140, 2200, 2658, 3218
BseRI GAGGAG 3 cut(s) 1251, 2516, 3211
BseSI GKGCMC 3 cut(s) 125, 499, 741
BseXI GCAGC 9 cut(s) 904, 1450, 2494, 2749, 2786, 2789, 2830, 3251, 3406
BseYI CCCAGC 5 cut(s) 2261, 2338, 2780, 2821, 3447
BsgI GTGCAG 2 cut(s) 1241, 3071
Bsh1236I CGCG 2 cut(s) 165, 2895
Bsh1285I CGRYCG 1 cut(s) 136
BshNI GGYRCC 2 cut(s) 252, 2389
BsiEI CGRYCG 1 cut(s) 136
BsiHKAI GWGCWC 6 cut(s) 150, 393, 499, 1736, 1859, 1866
BsiSI CCGG 4 cut(s) 204, 373, 1077, 2815
BslFI GGGAC 3 cut(s) 355, 1688, 3625
BslI CCNNNNNNNGG 9 cut(s) 37, 106, 164, 436, 613, 823, 2433, 2717, 2821
BsmAI GTCTC 8 cut(s) 11, 205, 457, 1041, 1577, 2562, 3274, 3359
BsmBI CGTCTC 1 cut(s) 205
BsmFI GGGAC 3 cut(s) 355, 1688, 3625
BsmI GAATGC 2 cut(s) 559, 2263
Bso31I GGTCTC 3 cut(s) 11, 1577, 3359
Bsp1286I GDGCHC 9 cut(s) 125, 150, 393, 499, 741, 1736, 1859, 1866, 2975
Bsp1720I GCTNAGC 1 cut(s) 44
Bsp19I CCATGG 2 cut(s) 65, 1618
BspFNI CGCG 2 cut(s) 165, 2895
BspHI TCATGA 3 cut(s) 307, 925, 2439
BspLI GGNNCC 7 cut(s) 157, 254, 1168, 1694, 2391, 2744, 2972
BspMAI CTGCAG 6 cut(s) 526, 1406, 1440, 2512, 2581, 3417
BspMI ACCTGC 2 cut(s) 1443, 2321
BspOI GCTAGC 1 cut(s) 3239
BspPI GGATC 7 cut(s) 77, 91, 450, 474, 1758, 2637, 3616
BspQI GCTCTTC 2 cut(s) 1337, 2055
BspT107I GGYRCC 2 cut(s) 252, 2389
BspTI CTTAAG 2 cut(s) 3017, 3191
BspTNI GGTCTC 3 cut(s) 11, 1577, 3359
BsrDI GCAATG 3 cut(s) 1512, 1741, 2805
BsrFI RCCGGY 1 cut(s) 2814
BsrI ACTGG 9 cut(s) 333, 400, 847, 1393, 2030, 2140, 2200, 2658, 3218
BssAI RCCGGY 1 cut(s) 2814
BssECI CCNNGG 7 cut(s) 65, 163, 317, 435, 1618, 2922, 3511
BssNI GRCGYC 1 cut(s) 168
BssT1I CCWWGG 5 cut(s) 65, 317, 435, 1618, 2922
Bst2UI CCWGG 5 cut(s) 1835, 1874, 2718, 3005, 3352
Bst6I CTCTTC 7 cut(s) 270, 1337, 1817, 2055, 2388, 2610, 3003
BstACI GRCGYC 1 cut(s) 168
BstAFI CTTAAG 2 cut(s) 3017, 3191
BstAPI GCANNNNNTGC 2 cut(s) 3398, 3412
BstC8I GCNNGC 7 cut(s) 522, 1251, 2816, 3025, 3237, 3417, 3575
BstDSI CCRYGG 3 cut(s) 65, 163, 1618
BstENI CCTNNNNNAGG 1 cut(s) 2431
BstFNI CGCG 2 cut(s) 165, 2895
BstH2I RGCGCY 1 cut(s) 1350
BstHHI GCGC 3 cut(s) 520, 1349, 2897
BstMAI GTCTC 8 cut(s) 11, 205, 457, 1041, 1577, 2562, 3274, 3359
BstMCI CGRYCG 1 cut(s) 136
BstMWI GCNNNNNNNGC 8 cut(s) 54, 162, 1883, 2513, 2684, 2771, 3398, 3412
BstNI CCWGG 5 cut(s) 1835, 1874, 2718, 3005, 3352
BstNSI RCATGY 1 cut(s) 2774
BstSCI CCNGG 7 cut(s) 371, 1075, 1833, 1872, 2716, 3003, 3350
BstSLI GKGCMC 3 cut(s) 125, 499, 741
BstUI CGCG 2 cut(s) 165, 2895
BstV1I GCAGC 9 cut(s) 904, 1450, 2494, 2749, 2786, 2789, 2830, 3251, 3406
BstV2I GAAGAC 4 cut(s) 74, 839, 1618, 1655
BstX2I RGATCY 5 cut(s) 69, 442, 2629, 2638, 3608
BstYI RGATCY 5 cut(s) 69, 442, 2629, 2638, 3608
Bsu36I CCTNAGG 1 cut(s) 1911
BsuI GTATCC 1 cut(s) 824
BtgI CCRYGG 3 cut(s) 65, 163, 1618
BtsI GCAGTG 1 cut(s) 2586
BtsIMutI CAGTG 8 cut(s) 340, 393, 988, 1684, 2023, 2586, 2651, 3253
BveI ACCTGC 2 cut(s) 1443, 2321
Cac8I GCNNGC 7 cut(s) 522, 1251, 2816, 3025, 3237, 3417, 3575
CaiI CAGNNNCTG 3 cut(s) 2516, 2780, 2912
CciI TCATGA 3 cut(s) 307, 925, 2439
CfoI GCGC 3 cut(s) 520, 1349, 2897
Cfr10I RCCGGY 1 cut(s) 2814
Cfr13I GGNCC 7 cut(s) 155, 627, 1074, 1247, 1692, 2197, 2424
Cfr42I CCGCGG 1 cut(s) 166
CseI GACGC 1 cut(s) 157
CsiI ACCWGGT 1 cut(s) 1833
Csp6I GTAC 8 cut(s) 291, 313, 997, 1483, 1572, 1828, 2390, 3582
CviQI GTAC 8 cut(s) 291, 313, 997, 1483, 1572, 1828, 2390, 3582
DraI TTTAAA 2 cut(s) 949, 2992
DrdI GACNNNNNNGTC 1 cut(s) 1622
DseDI GACNNNNNNGTC 1 cut(s) 1622
EaeI YGGCCR 1 cut(s) 1870
Eam1104I CTCTTC 7 cut(s) 270, 1337, 1817, 2055, 2388, 2610, 3003
EarI CTCTTC 7 cut(s) 270, 1337, 1817, 2055, 2388, 2610, 3003
Ecl136II GAGCTC 2 cut(s) 1857, 1864
Eco130I CCWWGG 5 cut(s) 65, 317, 435, 1618, 2922
Eco24I GRGCYC 3 cut(s) 1859, 1866, 2975
Eco31I GGTCTC 3 cut(s) 11, 1577, 3359
Eco47I GGWCC 1 cut(s) 1692
Eco53kI GAGCTC 2 cut(s) 1857, 1864
Eco57I CTGAAG 4 cut(s) 833, 1838, 2196, 2385
Eco81I CCTNAGG 1 cut(s) 1911
EcoICRI GAGCTC 2 cut(s) 1857, 1864
EcoNI CCTNNNNNAGG 1 cut(s) 2431
EcoO109I RGGNCCY 1 cut(s) 1692
EcoRII CCWGG 5 cut(s) 1833, 1872, 2716, 3003, 3350
EcoT14I CCWWGG 5 cut(s) 65, 317, 435, 1618, 2922
EcoT22I ATGCAT 2 cut(s) 2086, 3619
EcoT38I GRGCYC 3 cut(s) 1859, 1866, 2975
ErhI CCWWGG 5 cut(s) 65, 317, 435, 1618, 2922
Esp3I CGTCTC 1 cut(s) 205
FalI AAGNNNNNCTT 6 cut(s) 672, 704, 1796, 1828, 3000, 3032
FaqI GGGAC 3 cut(s) 355, 1688, 3625
FauI CCCGC 2 cut(s) 200, 3016
FauNDI CATATG 2 cut(s) 2281, 3496
FriOI GRGCYC 3 cut(s) 1859, 1866, 2975
FspBI CTAG 4 cut(s) 1964, 2397, 3236, 3284
GlaI GCGC 3 cut(s) 519, 1348, 2896
GsaI CCCAGC 5 cut(s) 2265, 2342, 2784, 2825, 3451
GsuI CTGGAG 5 cut(s) 684, 1263, 2675, 2700, 3248
HaeII RGCGCY 1 cut(s) 1350
HapII CCGG 4 cut(s) 204, 373, 1077, 2815
HgaI GACGC 1 cut(s) 157
HhaI GCGC 3 cut(s) 520, 1349, 2897
Hin1I GRCGYC 1 cut(s) 168
Hin6I GCGC 3 cut(s) 518, 1347, 2895
HinP1I GCGC 3 cut(s) 518, 1347, 2895
HincII GTYRAC 1 cut(s) 3227
HindII GTYRAC 1 cut(s) 3227
HindIII AAGCTT 1 cut(s) 3014
HpaII CCGG 4 cut(s) 204, 373, 1077, 2815
HphI GGTGA 5 cut(s) 1223, 2237, 2345, 2367, 3241
Hpy166II GTNNAC 4 cut(s) 261, 497, 724, 3227
Hpy8I GTNNAC 4 cut(s) 261, 497, 724, 3227
HpyCH4IV ACGT 1 cut(s) 411
HpyF10VI GCNNNNNNNGC 8 cut(s) 54, 162, 1883, 2513, 2684, 2771, 3398, 3412
HpySE526I ACGT 1 cut(s) 411
Hsp92I GRCGYC 1 cut(s) 168
HspAI GCGC 3 cut(s) 518, 1347, 2895
KpnI GGTACC 1 cut(s) 2393
KroI GCCGGC 1 cut(s) 2814
KroNI GCCGGC 1 cut(s) 2816
KspI CCGCGG 1 cut(s) 166
LguI GCTCTTC 2 cut(s) 1337, 2055
Lsp1109I GCAGC 9 cut(s) 904, 1450, 2494, 2749, 2786, 2789, 2830, 3251, 3406
MabI ACCWGGT 1 cut(s) 1833
MaeI CTAG 4 cut(s) 1964, 2397, 3236, 3284
MaeII ACGT 1 cut(s) 411
MaeIII GTNAC 7 cut(s) 26, 2131, 2225, 2373, 3257, 3341, 3624
MfeI CAATTG 2 cut(s) 1773, 2852
MflI RGATCY 5 cut(s) 69, 442, 2629, 2638, 3608
MhlI GDGCHC 9 cut(s) 125, 150, 393, 499, 741, 1736, 1859, 1866, 2975
MlsI TGGCCA 1 cut(s) 1872
MluNI TGGCCA 1 cut(s) 1872
MlyI GAGTC 5 cut(s) 88, 553, 828, 1939, 2887
MmeI TCCRAC 1 cut(s) 3586
Mox20I TGGCCA 1 cut(s) 1872
Mph1103I ATGCAT 2 cut(s) 2086, 3619
MroNI GCCGGC 1 cut(s) 2814
MroXI GAANNNNTTC 3 cut(s) 1904, 2556, 3168
MscI TGGCCA 1 cut(s) 1872
MslI CAYNNNNRTG 3 cut(s) 90, 930, 3305
Msp20I TGGCCA 1 cut(s) 1872
MspA1I CMGCKG 6 cut(s) 90, 165, 1253, 2780, 2821, 3338
MspCI CTTAAG 2 cut(s) 3017, 3191
MspI CCGG 4 cut(s) 204, 373, 1077, 2815
MspR9I CCNGG 7 cut(s) 373, 1077, 1835, 1874, 2718, 3005, 3352
MunI CAATTG 2 cut(s) 1773, 2852
Mva1269I GAATGC 2 cut(s) 559, 2263
MvaI CCWGG 5 cut(s) 1835, 1874, 2718, 3005, 3352
MvnI CGCG 2 cut(s) 165, 2895
MwoI GCNNNNNNNGC 8 cut(s) 54, 162, 1883, 2513, 2684, 2771, 3398, 3412
NaeI GCCGGC 1 cut(s) 2816
NciI CCSGG 2 cut(s) 373, 1077
NcoI CCATGG 2 cut(s) 65, 1618
NdeI CATATG 2 cut(s) 2281, 3496
NgoMIV GCCGGC 1 cut(s) 2814
NheI GCTAGC 1 cut(s) 3235
NlaIV GGNNCC 7 cut(s) 157, 254, 1168, 1694, 2391, 2744, 2972
NmuCI GTSAC 2 cut(s) 2225, 2373
NsiI ATGCAT 2 cut(s) 2086, 3619
NspI RCATGY 1 cut(s) 2774
OliI CACNNNNGTG 1 cut(s) 90
PagI TCATGA 3 cut(s) 307, 925, 2439
PaqCI CACCTGC 1 cut(s) 2321
PciSI GCTCTTC 2 cut(s) 1337, 2055
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PctI GAATGC 2 cut(s) 559, 2263
PdiI GCCGGC 1 cut(s) 2816
PdmI GAANNNNTTC 3 cut(s) 1904, 2556, 3168
PfeI GAWTC 5 cut(s) 883, 989, 1093, 1683, 2948
PflFI GACNNNGTC 1 cut(s) 543
PfoI TCCNGGA 1 cut(s) 371
PleI GAGTC 5 cut(s) 87, 552, 827, 1939, 2886
PpsI GAGTC 5 cut(s) 87, 552, 827, 1939, 2886
PpuMI RGGWCCY 1 cut(s) 1692
PshBI ATTAAT 1 cut(s) 3146
PsiI TTATAA 1 cut(s) 240
Psp124BI GAGCTC 2 cut(s) 1859, 1866
Psp5II RGGWCCY 1 cut(s) 1692
Psp6I CCWGG 5 cut(s) 1833, 1872, 2716, 3003, 3350
PspFI CCCAGC 5 cut(s) 2261, 2338, 2780, 2821, 3447
PspGI CCWGG 5 cut(s) 1833, 1872, 2716, 3003, 3350
PspN4I GGNNCC 7 cut(s) 157, 254, 1168, 1694, 2391, 2744, 2972
PspPI GGNCC 7 cut(s) 155, 627, 1074, 1247, 1692, 2197, 2424
PspPPI RGGWCCY 1 cut(s) 1692
PstI CTGCAG 6 cut(s) 526, 1406, 1440, 2512, 2581, 3417
PstNI CAGNNNCTG 3 cut(s) 2516, 2780, 2912
PsuI RGATCY 5 cut(s) 69, 442, 2629, 2638, 3608
PsyI GACNNNGTC 1 cut(s) 543
PvuII CAGCTG 3 cut(s) 2780, 2821, 3338
RsaI GTAC 8 cut(s) 292, 314, 998, 1484, 1573, 1829, 2391, 3583
RsaNI GTAC 8 cut(s) 291, 313, 997, 1483, 1572, 1828, 2390, 3582
RseI CAYNNNNRTG 3 cut(s) 90, 930, 3305
SacI GAGCTC 2 cut(s) 1859, 1866
SacII CCGCGG 1 cut(s) 166
SapI GCTCTTC 2 cut(s) 1337, 2055
Sau96I GGNCC 7 cut(s) 155, 627, 1074, 1247, 1692, 2197, 2424
SbfI CCTGCAGG 1 cut(s) 3417
SchI GAGTC 5 cut(s) 88, 553, 828, 1939, 2887
ScrFI CCNGG 7 cut(s) 373, 1077, 1835, 1874, 2718, 3005, 3352
SdaI CCTGCAGG 1 cut(s) 3417
SduI GDGCHC 9 cut(s) 125, 150, 393, 499, 741, 1736, 1859, 1866, 2975
SexAI ACCWGGT 1 cut(s) 1833
Sfr303I CCGCGG 1 cut(s) 166
SgrBI CCGCGG 1 cut(s) 166
SinI GGWCC 1 cut(s) 1692
SmiMI CAYNNNNRTG 3 cut(s) 90, 930, 3305
SmlI CTYRAG 3 cut(s) 3017, 3191, 3277
SmoI CTYRAG 3 cut(s) 3017, 3191, 3277
Sse8387I CCTGCAGG 1 cut(s) 3417
SspI AATATT 1 cut(s) 2451
SspMI CTAG 4 cut(s) 1964, 2397, 3236, 3284
SstI GAGCTC 2 cut(s) 1859, 1866
StyD4I CCNGG 7 cut(s) 371, 1075, 1833, 1872, 2716, 3003, 3350
StyI CCWWGG 5 cut(s) 65, 317, 435, 1618, 2922
TaiI ACGT 1 cut(s) 414
TaqI TCGA 6 cut(s) 15, 132, 676, 1267, 1753, 2903
TatI WGTACW 2 cut(s) 996, 3581
TauI GCSGC 1 cut(s) 168
TfiI GAWTC 5 cut(s) 883, 989, 1093, 1683, 2948
TscAI CASTG 8 cut(s) 340, 400, 988, 1684, 2030, 2586, 2658, 3260
TseFI GTSAC 2 cut(s) 2225, 2373
TseI GCWGC 9 cut(s) 917, 1438, 2507, 2762, 2774, 2777, 2818, 3239, 3419
Tsp45I GTSAC 2 cut(s) 2225, 2373
TspGWI ACGGA 6 cut(s) 278, 1172, 1461, 2580, 2686, 3627
TspRI CASTG 8 cut(s) 340, 400, 988, 1684, 2030, 2586, 2658, 3260
Tth111I GACNNNGTC 1 cut(s) 543
Vha464I CTTAAG 2 cut(s) 3017, 3191
VneI GTGCAC 1 cut(s) 495
VpaK11BI GGWCC 1 cut(s) 1692
VspI ATTAAT 1 cut(s) 3146
XagI CCTNNNNNAGG 1 cut(s) 2431
XapI RAATTY 6 cut(s) 11, 2101, 2736, 2785, 2993, 3050
XceI RCATGY 1 cut(s) 2774
XcmI CCANNNNNNNNNTGG 2 cut(s) 1744, 3358
XmnI GAANNNNTTC 3 cut(s) 1904, 2556, 3168
XspI CTAG 4 cut(s) 1964, 2397, 3236, 3284
Zsp2I ATGCAT 2 cut(s) 2086, 3619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.