RLG00000033392

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0019302
Physical Location & Seq
Forward (+)
26569244 .. 26570941
1698 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033392

Sequence Viewer

Length: 804 bp
ATGGCTATAAAATTGACAACAAAGAAAGCCATAAAACACACTAACATTGACGTTAGTACGTTAACGTCAACACTGAATCTCCACATCTGGTTCGTCTCCTCGCATCTCCCTCTCTCTCTTCGCCCATTTTCCTCCTTCCCCTCACCATCTAGTCGTGAACTGAATCGAACAAGATCATGGTGGAAGTCGTCGGAGCTGAAGAAGCTGGTGTCATGGTCATGGTGGAAGTATTGCTCAACGGGAACCAGAACAGGAACTAGATCGAATTTCAAGATGACTACTTTGTTGATGCGTTTTCTCAGGTCTTTACTCATGGCAAATGCTGGAGATTGCAGGGCTATGTTGTCACGAAATGGAGCAGCTATAGAAATGTCAAAAGATCATAGACCCTGCTGCAAAAAAGAAAGGACGCGAATTGAGTCTTTGGGTGGATATGTTGATGATGGTTATCTGAATAGTCAGTTAGGGGTCACCCGTGCATTAGGTGATTGGCATCTTGAAGGATTGAAGGATGAGGTTGAAAGGGGAGGACCCTTGAGTGCTGAACCGGAACTTAAATTGACAACACTGACCAAGGATGATGAATTTTTTATCATTGGTAGTGATGGAATATGGGATGTGTTCACCAACGAAAATGCTGTAGACTTTGCTCGAAGGAAACTCCAAGAGCACAATGATGTGAAGTTGTGTTGCAAGCAAATAGTAGAGGAGGCAATAAAACGAGGCGCAACCGACAATTTGACACTTGTTATGGTAGAGTTTCCACTTGGAGCCACCTCCACATGTGGTGGTAGAAAGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

268

Amino Acids

30.12

Weight (kDa)

9.25

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 101 - 246 1.6e-47 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019302)

Species Orthologous Gene IDs
prunus_persica Prupe.7G255800_v2.0.a1
rosa_laevigata RLG00000009207 RLG00000016426 RLG00000032266 RLG00000033392
rosa_multiflora Rmu_sc0001981.1_g000055
rosa_samantha Rh6AG178800 Rh6AG181000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 642
AccII CGCG 1 cut(s) 412
AcsI RAATTY 2 cut(s) 265, 584
AcuI CTGAAG 1 cut(s) 218
AfaI GTAC 1 cut(s) 58
AflIII ACRYGT 1 cut(s) 782
AgsI TTSAA 4 cut(s) 271, 500, 508, 521
AluBI AGCT 3 cut(s) 196, 205, 362
AluI AGCT 3 cut(s) 196, 205, 362
Alw21I GWGCWC 1 cut(s) 672
Alw26I GTCTC 1 cut(s) 100
ApeKI GCWGC 2 cut(s) 359, 393
ApoI RAATTY 2 cut(s) 265, 584
AspLEI GCGC 1 cut(s) 728
AspS9I GGNCC 1 cut(s) 530
AsuHPI GGTGA 4 cut(s) 135, 463, 497, 616
AvaII GGWCC 1 cut(s) 530
Bbv12I GWGCWC 1 cut(s) 672
BbvI GCAGC 2 cut(s) 371, 380
BccI CCATC 3 cut(s) 154, 437, 599
BcoDI GTCTC 1 cut(s) 100
BfaI CTAG 3 cut(s) 150, 258, 802
BfmI CTRYAG 2 cut(s) 363, 639
BisI GCNGC 2 cut(s) 360, 394
BlsI GCNGC 2 cut(s) 361, 395
Bme18I GGWCC 1 cut(s) 530
BmgT120I GGNCC 1 cut(s) 530
BmiI GGNNCC 3 cut(s) 244, 532, 772
BmsI GCATC 3 cut(s) 112, 279, 502
BpmI CTGGAG 1 cut(s) 345
BpuEI CTTGAG 1 cut(s) 556
BsaBI GATNNNNATC 2 cut(s) 447, 492
BsaJI CCNNGG 1 cut(s) 573
BsaWI WCCGGW 1 cut(s) 547
BsaXI ACNNNNNCTCC 2 cut(s) 63, 93
Bse8I GATNNNNATC 2 cut(s) 447, 492
BseDI CCNNGG 1 cut(s) 573
BseGI GGATG 3 cut(s) 517, 583, 622
BseJI GATNNNNATC 2 cut(s) 447, 492
BseMII CTCAG 1 cut(s) 313
BseRI GAGGAG 2 cut(s) 88, 722
BseXI GCAGC 2 cut(s) 371, 380
Bsh1236I CGCG 1 cut(s) 412
BsiHKAI GWGCWC 1 cut(s) 672
BsiSI CCGG 1 cut(s) 548
BsmAI GTCTC 1 cut(s) 100
BsmBI CGTCTC 1 cut(s) 100
Bsp1286I GDGCHC 1 cut(s) 672
Bsp143I GATC 3 cut(s) 173, 260, 379
BspCNI CTCAG 1 cut(s) 312
BspFNI CGCG 1 cut(s) 412
BspLI GGNNCC 3 cut(s) 244, 532, 772
BssECI CCNNGG 1 cut(s) 573
BssMI GATC 3 cut(s) 173, 260, 379
BssT1I CCWWGG 1 cut(s) 573
Bst6I CTCTTC 1 cut(s) 123
BstC8I GCNNGC 1 cut(s) 695
BstDEI CTNAG 1 cut(s) 299
BstEII GGTNACC 1 cut(s) 469
BstF5I GGATG 3 cut(s) 517, 583, 622
BstFNI CGCG 1 cut(s) 412
BstHHI GCGC 1 cut(s) 728
BstKTI GATC 3 cut(s) 176, 263, 382
BstMAI GTCTC 1 cut(s) 100
BstMBI GATC 3 cut(s) 173, 260, 379
BstMWI GCNNNNNNNGC 1 cut(s) 202
BstNSI RCATGY 1 cut(s) 786
BstPI GGTNACC 1 cut(s) 469
BstSFI CTRYAG 2 cut(s) 363, 639
BstUI CGCG 1 cut(s) 412
BstV1I GCAGC 2 cut(s) 371, 380
BtsCI GGATG 3 cut(s) 517, 583, 622
BtsIMutI CAGTG 2 cut(s) 71, 566
Cac8I GCNNGC 1 cut(s) 695
CfoI GCGC 1 cut(s) 728
Cfr13I GGNCC 1 cut(s) 530
CseI GACGC 1 cut(s) 418
Csp6I GTAC 1 cut(s) 57
CviAII CATG 5 cut(s) 177, 213, 219, 313, 783
CviJI RGCY 7 cut(s) 5, 29, 196, 205, 338, 362, 773
CviKI_1 RGCY 7 cut(s) 5, 29, 196, 205, 338, 362, 773
CviQI GTAC 1 cut(s) 57
DdeI CTNAG 1 cut(s) 299
DpnI GATC 3 cut(s) 175, 262, 381
DpnII GATC 3 cut(s) 173, 260, 379
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Eco130I CCWWGG 1 cut(s) 573
Eco47I GGWCC 1 cut(s) 530
Eco57I CTGAAG 1 cut(s) 218
Eco91I GGTNACC 1 cut(s) 469
EcoO109I RGGNCCY 1 cut(s) 530
EcoO65I GGTNACC 1 cut(s) 469
EcoT14I CCWWGG 1 cut(s) 573
ErhI CCWWGG 1 cut(s) 573
Esp3I CGTCTC 1 cut(s) 100
FaeI CATG 5 cut(s) 180, 216, 222, 316, 786
FatI CATG 5 cut(s) 176, 212, 218, 312, 782
FblI GTMKAC 1 cut(s) 642
Fnu4HI GCNGC 2 cut(s) 360, 394
FokI GGATG 3 cut(s) 524, 590, 629
Fsp4HI GCNGC 2 cut(s) 360, 394
FspBI CTAG 3 cut(s) 150, 258, 802
GlaI GCGC 1 cut(s) 727
GluI GCNGC 2 cut(s) 360, 394
GsuI CTGGAG 1 cut(s) 345
HapII CCGG 1 cut(s) 548
HgaI GACGC 1 cut(s) 418
HhaI GCGC 1 cut(s) 728
Hin1II CATG 5 cut(s) 180, 216, 222, 316, 786
Hin6I GCGC 1 cut(s) 726
HinP1I GCGC 1 cut(s) 726
HincII GTYRAC 2 cut(s) 63, 69
HindII GTYRAC 2 cut(s) 63, 69
HinfI GANTC 3 cut(s) 76, 163, 419
HpaI GTTAAC 1 cut(s) 63
HpaII CCGG 1 cut(s) 548
HphI GGTGA 4 cut(s) 135, 463, 497, 616
Hpy166II GTNNAC 5 cut(s) 63, 69, 158, 624, 643
Hpy188I TCNGA 2 cut(s) 193, 453
Hpy188III TCNNGA 4 cut(s) 155, 271, 348, 497
Hpy8I GTNNAC 5 cut(s) 63, 69, 158, 624, 643
Hpy99I CGWCG 1 cut(s) 193
HpyAV CCTTC 4 cut(s) 145, 494, 502, 648
HpyCH4IV ACGT 3 cut(s) 51, 59, 65
HpyCH4V TGCA 4 cut(s) 333, 396, 479, 693
HpyF10VI GCNNNNNNNGC 1 cut(s) 202
HpyF3I CTNAG 1 cut(s) 299
HpySE526I ACGT 3 cut(s) 51, 59, 65
Hsp92II CATG 5 cut(s) 180, 216, 222, 316, 786
HspAI GCGC 1 cut(s) 726
KspAI GTTAAC 1 cut(s) 63
Kzo9I GATC 3 cut(s) 173, 260, 379
LmnI GCTCC 3 cut(s) 193, 356, 770
LpnPI CCDG 9 cut(s) 73, 191, 237, 259, 286, 309, 319, 403, 561
Lsp1109I GCAGC 2 cut(s) 371, 380
LweI GCATC 3 cut(s) 112, 279, 502
MaeI CTAG 3 cut(s) 150, 258, 802
MaeII ACGT 3 cut(s) 51, 59, 65
MaeIII GTNAC 2 cut(s) 345, 469
MalI GATC 3 cut(s) 175, 262, 381
MboI GATC 3 cut(s) 173, 260, 379
MboII GAAGA 2 cut(s) 110, 211
MhlI GDGCHC 1 cut(s) 672
MluCI AATT 6 cut(s) 11, 265, 414, 557, 584, 736
MlyI GAGTC 1 cut(s) 428
MmeI TCCRAC 1 cut(s) 171
MseI TTAA 2 cut(s) 62, 555
MslI CAYNNNNRTG 2 cut(s) 217, 675
MspI CCGG 1 cut(s) 548
MvnI CGCG 1 cut(s) 412
MwoI GCNNNNNNNGC 1 cut(s) 202
NdeII GATC 3 cut(s) 173, 260, 379
NlaIII CATG 5 cut(s) 180, 216, 222, 316, 786
NlaIV GGNNCC 3 cut(s) 244, 532, 772
NmuCI GTSAC 2 cut(s) 345, 469
NspI RCATGY 1 cut(s) 786
PciI ACATGT 1 cut(s) 782
PfeI GAWTC 2 cut(s) 76, 163
PkrI GCNGC 2 cut(s) 361, 395
PleI GAGTC 1 cut(s) 427
PpsI GAGTC 1 cut(s) 427
PpuMI RGGWCCY 1 cut(s) 530
PscI ACATGT 1 cut(s) 782
Psp5II RGGWCCY 1 cut(s) 530
PspEI GGTNACC 1 cut(s) 469
PspN4I GGNNCC 3 cut(s) 244, 532, 772
PspPI GGNCC 1 cut(s) 530
PspPPI RGGWCCY 1 cut(s) 530
RsaI GTAC 1 cut(s) 58
RsaNI GTAC 1 cut(s) 57
RseI CAYNNNNRTG 2 cut(s) 217, 675
SaqAI TTAA 2 cut(s) 62, 555
SatI GCNGC 2 cut(s) 360, 394
Sau3AI GATC 3 cut(s) 173, 260, 379
Sau96I GGNCC 1 cut(s) 530
SchI GAGTC 1 cut(s) 428
SduI GDGCHC 1 cut(s) 672
SfaNI GCATC 3 cut(s) 112, 279, 502
SfcI CTRYAG 2 cut(s) 363, 639
SinI GGWCC 1 cut(s) 530
SmiMI CAYNNNNRTG 2 cut(s) 217, 675
SmlI CTYRAG 1 cut(s) 535
SmoI CTYRAG 1 cut(s) 535
Sse9I AATT 6 cut(s) 11, 265, 414, 557, 584, 736
SspMI CTAG 3 cut(s) 150, 258, 802
StyI CCWWGG 1 cut(s) 573
TaiI ACGT 3 cut(s) 54, 62, 68
TaqI TCGA 3 cut(s) 166, 263, 652
TasI AATT 6 cut(s) 11, 265, 414, 557, 584, 736
TfiI GAWTC 2 cut(s) 76, 163
Tru1I TTAA 2 cut(s) 62, 555
Tru9I TTAA 2 cut(s) 62, 555
TscAI CASTG 2 cut(s) 78, 573
TseFI GTSAC 2 cut(s) 345, 469
TseI GCWGC 2 cut(s) 359, 393
Tsp45I GTSAC 2 cut(s) 345, 469
TspDTI ATGAA 1 cut(s) 597
TspRI CASTG 2 cut(s) 78, 573
VpaK11BI GGWCC 1 cut(s) 530
XapI RAATTY 2 cut(s) 265, 584
XceI RCATGY 1 cut(s) 786
XmiI GTMKAC 1 cut(s) 642
XspI CTAG 3 cut(s) 150, 258, 802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.