Rh6AG181000

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0019302
Physical Location & Seq
Forward (+)
31589129 .. 31591348
2220 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG181000.1

Sequence Viewer

Length: 531 bp
ATGACTACTTTGTTGATGTGTTTTCTCAGGTCTTTGCTCGTGGCAAATGCTGGAGATTGCAGGGCTATATTGTCACGAAATGGAGCAGCTATAGAAATGTCAAAAGATCATAGACCCTGCTGCAAAAAAGAAAGGACGCGAATTGAGTCTTTGGGTGGATATGTTGATGATGGTTATCTGAATGGTCAGTTAGGGGTCACCCGTGCATTAGGTGATTGGCATCTTGAAGGATTGAAGGATGAGGGTGAAAGGGGAGGACCCTTGAGTGCTGAACCGGAACTTAAATTGACAACACTGACCAAGGATGATGAATTTTTTATCATTGGTAGTGATGGAATATGGGATGTGTTCACCAACGAAAATGCTGTAGACTTTGCTCGAAGGAAACTCCAAGAGCACAATGATGTGAAGCTGTGTTGCAAGCACATAGTAGAGGAGGTAATAAAACGAGGCGCAACCGACAATTTGACACTTGTTATGGTAGAGTTTCCACTTGGAGCCACCTCCACATGTGGTGGTAGAAAGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

176

Amino Acids

19.43

Weight (kDa)

5.62

Isoelectric Point (pI)

31.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 10 - 155 4.2e-48 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019302)

Species Orthologous Gene IDs
prunus_persica Prupe.7G255800_v2.0.a1
rosa_laevigata RLG00000009207 RLG00000016426 RLG00000032266 RLG00000033392
rosa_multiflora Rmu_sc0001981.1_g000055
rosa_samantha Rh6AG178800 Rh6AG181000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 369
AccII CGCG 1 cut(s) 139
AcsI RAATTY 1 cut(s) 311
AflIII ACRYGT 1 cut(s) 509
AgsI TTSAA 2 cut(s) 227, 235
AluBI AGCT 2 cut(s) 89, 412
AluI AGCT 2 cut(s) 89, 412
Alw21I GWGCWC 1 cut(s) 399
ApeKI GCWGC 2 cut(s) 86, 120
ApoI RAATTY 1 cut(s) 311
AspLEI GCGC 1 cut(s) 455
AspS9I GGNCC 1 cut(s) 257
AsuHPI GGTGA 4 cut(s) 190, 224, 257, 343
AvaII GGWCC 1 cut(s) 257
BauI CACGAG 1 cut(s) 38
Bbv12I GWGCWC 1 cut(s) 399
BbvI GCAGC 2 cut(s) 98, 107
BccI CCATC 2 cut(s) 164, 326
BfaI CTAG 1 cut(s) 529
BfmI CTRYAG 2 cut(s) 90, 366
BisI GCNGC 2 cut(s) 87, 121
BlsI GCNGC 2 cut(s) 88, 122
Bme18I GGWCC 1 cut(s) 257
BmgT120I GGNCC 1 cut(s) 257
BmiI GGNNCC 2 cut(s) 259, 499
BmsI GCATC 1 cut(s) 229
BpmI CTGGAG 1 cut(s) 72
BpuEI CTTGAG 1 cut(s) 283
BsaBI GATNNNNATC 2 cut(s) 174, 219
BsaJI CCNNGG 1 cut(s) 300
BsaWI WCCGGW 1 cut(s) 274
Bse8I GATNNNNATC 2 cut(s) 174, 219
BseDI CCNNGG 1 cut(s) 300
BseGI GGATG 3 cut(s) 244, 310, 349
BseJI GATNNNNATC 2 cut(s) 174, 219
BseMII CTCAG 1 cut(s) 40
BseRI GAGGAG 1 cut(s) 449
BseXI GCAGC 2 cut(s) 98, 107
Bsh1236I CGCG 1 cut(s) 139
BsiHKAI GWGCWC 1 cut(s) 399
BsiSI CCGG 1 cut(s) 275
Bsp1286I GDGCHC 1 cut(s) 399
Bsp143I GATC 1 cut(s) 106
BspCNI CTCAG 1 cut(s) 39
BspFNI CGCG 1 cut(s) 139
BspLI GGNNCC 2 cut(s) 259, 499
BssECI CCNNGG 1 cut(s) 300
BssMI GATC 1 cut(s) 106
BssSI CACGAG 1 cut(s) 38
BssT1I CCWWGG 1 cut(s) 300
Bst2BI CACGAG 1 cut(s) 38
BstC8I GCNNGC 1 cut(s) 422
BstDEI CTNAG 1 cut(s) 26
BstEII GGTNACC 1 cut(s) 196
BstF5I GGATG 3 cut(s) 244, 310, 349
BstFNI CGCG 1 cut(s) 139
BstHHI GCGC 1 cut(s) 455
BstKTI GATC 1 cut(s) 109
BstMBI GATC 1 cut(s) 106
BstNSI RCATGY 1 cut(s) 513
BstPI GGTNACC 1 cut(s) 196
BstSFI CTRYAG 2 cut(s) 90, 366
BstUI CGCG 1 cut(s) 139
BstV1I GCAGC 2 cut(s) 98, 107
BtsCI GGATG 3 cut(s) 244, 310, 349
BtsIMutI CAGTG 1 cut(s) 293
Cac8I GCNNGC 1 cut(s) 422
CfoI GCGC 1 cut(s) 455
Cfr13I GGNCC 1 cut(s) 257
CseI GACGC 1 cut(s) 145
CviAII CATG 1 cut(s) 510
CviJI RGCY 4 cut(s) 65, 89, 412, 500
CviKI_1 RGCY 4 cut(s) 65, 89, 412, 500
DdeI CTNAG 1 cut(s) 26
DpnI GATC 1 cut(s) 108
DpnII GATC 1 cut(s) 106
Eco130I CCWWGG 1 cut(s) 300
Eco47I GGWCC 1 cut(s) 257
Eco91I GGTNACC 1 cut(s) 196
EcoO109I RGGNCCY 1 cut(s) 257
EcoO65I GGTNACC 1 cut(s) 196
EcoT14I CCWWGG 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 300
FaeI CATG 1 cut(s) 513
FaiI YATR 8 cut(s) 68, 92, 111, 162, 340, 428, 479, 511
FatI CATG 1 cut(s) 509
FblI GTMKAC 1 cut(s) 369
Fnu4HI GCNGC 2 cut(s) 87, 121
FokI GGATG 3 cut(s) 251, 317, 356
Fsp4HI GCNGC 2 cut(s) 87, 121
FspBI CTAG 1 cut(s) 529
GlaI GCGC 1 cut(s) 454
GluI GCNGC 2 cut(s) 87, 121
GsuI CTGGAG 1 cut(s) 72
HapII CCGG 1 cut(s) 275
HgaI GACGC 1 cut(s) 145
HhaI GCGC 1 cut(s) 455
Hin1II CATG 1 cut(s) 513
Hin6I GCGC 1 cut(s) 453
HinP1I GCGC 1 cut(s) 453
HinfI GANTC 1 cut(s) 146
HpaII CCGG 1 cut(s) 275
HphI GGTGA 4 cut(s) 190, 224, 257, 343
Hpy166II GTNNAC 2 cut(s) 351, 370
Hpy188I TCNGA 1 cut(s) 180
Hpy188III TCNNGA 2 cut(s) 75, 224
Hpy8I GTNNAC 2 cut(s) 351, 370
HpyAV CCTTC 3 cut(s) 221, 229, 375
HpyCH4V TGCA 4 cut(s) 60, 123, 206, 420
HpyF3I CTNAG 1 cut(s) 26
Hsp92II CATG 1 cut(s) 513
HspAI GCGC 1 cut(s) 453
Kzo9I GATC 1 cut(s) 106
LmnI GCTCC 2 cut(s) 83, 497
LpnPI CCDG 5 cut(s) 13, 36, 46, 130, 288
Lsp1109I GCAGC 2 cut(s) 98, 107
LweI GCATC 1 cut(s) 229
MaeI CTAG 1 cut(s) 529
MaeIII GTNAC 2 cut(s) 72, 196
MalI GATC 1 cut(s) 108
MboI GATC 1 cut(s) 106
MhlI GDGCHC 1 cut(s) 399
MluCI AATT 4 cut(s) 141, 284, 311, 463
MlyI GAGTC 1 cut(s) 155
MnlI CCTC 6 cut(s) 235, 248, 427, 430, 443, 514
MseI TTAA 1 cut(s) 282
MslI CAYNNNNRTG 1 cut(s) 402
MspI CCGG 1 cut(s) 275
MvnI CGCG 1 cut(s) 139
NdeII GATC 1 cut(s) 106
NlaIII CATG 1 cut(s) 513
NlaIV GGNNCC 2 cut(s) 259, 499
NmuCI GTSAC 2 cut(s) 72, 196
NspI RCATGY 1 cut(s) 513
PciI ACATGT 1 cut(s) 509
PkrI GCNGC 2 cut(s) 88, 122
PleI GAGTC 1 cut(s) 154
PpsI GAGTC 1 cut(s) 154
PpuMI RGGWCCY 1 cut(s) 257
PscI ACATGT 1 cut(s) 509
Psp5II RGGWCCY 1 cut(s) 257
PspEI GGTNACC 1 cut(s) 196
PspN4I GGNNCC 2 cut(s) 259, 499
PspPI GGNCC 1 cut(s) 257
PspPPI RGGWCCY 1 cut(s) 257
RseI CAYNNNNRTG 1 cut(s) 402
SaqAI TTAA 1 cut(s) 282
SatI GCNGC 2 cut(s) 87, 121
Sau3AI GATC 1 cut(s) 106
Sau96I GGNCC 1 cut(s) 257
SchI GAGTC 1 cut(s) 155
SduI GDGCHC 1 cut(s) 399
SetI ASST 7 cut(s) 32, 91, 214, 414, 441, 506, 528
SfaNI GCATC 1 cut(s) 229
SfcI CTRYAG 2 cut(s) 90, 366
SinI GGWCC 1 cut(s) 257
SmiMI CAYNNNNRTG 1 cut(s) 402
SmlI CTYRAG 1 cut(s) 262
SmoI CTYRAG 1 cut(s) 262
Sse9I AATT 4 cut(s) 141, 284, 311, 463
SspMI CTAG 1 cut(s) 529
StyI CCWWGG 1 cut(s) 300
TaqI TCGA 1 cut(s) 379
TasI AATT 4 cut(s) 141, 284, 311, 463
Tru1I TTAA 1 cut(s) 282
Tru9I TTAA 1 cut(s) 282
TscAI CASTG 1 cut(s) 300
TseFI GTSAC 2 cut(s) 72, 196
TseI GCWGC 2 cut(s) 86, 120
Tsp45I GTSAC 2 cut(s) 72, 196
TspDTI ATGAA 1 cut(s) 324
TspRI CASTG 1 cut(s) 300
VpaK11BI GGWCC 1 cut(s) 257
XapI RAATTY 1 cut(s) 311
XceI RCATGY 1 cut(s) 513
XmiI GTMKAC 1 cut(s) 369
XspI CTAG 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.