RLG00000033658

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0017815
Physical Location & Seq
Reverse (-)
30287299 .. 30289508
2210 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033658

Sequence Viewer

Length: 1563 bp
ATGGGAGCCTGGCTCCTTATACCCTTCTCCGTCTTCCTCACTTTCACCCTAAAGTTCATCCTCTTCCCCTACAAACCCTCCCACAAACTCAACGCCCCACTCCCTCCTGGTCCCACCACCATACCCGTCGTCGGCACCTTCATATGGCTCTTCAAACTCGCCTCCCGCGGCATTGAGCCCGTCCTCCGTGACCTGCATGCCACGTACGGCGGCATCGTCTCTGTCCCCGTCCCCTTCTCTTACCCCGTCATCATGATCGCCGACCGCTCCCTTGCCCACCAAGCCCTTGTCAACAACGGTGCCGTTTTTGCCGACTGCCCTGAGGTCCAACCATCCATCAAGTTCATCACCAGCAACCAACGCATCATCGCCACCGCTGGCTACGGCTCTACGTGGCGCCTCCTGCGCCGGAACCTCGTCTCTGAGCTCATGGTTCCGTCTCGGCTCAAGGCCTTGGCCGCTTCCCGGAAGTGGGCCCTAGACAGCCTCGCCAGCAGTCTTGCAACGTCGTCTTCATCGGGACGTAACATGGTGGGAGTTTTGGATCATTTCCGGATGTCCATGACGGGTTTGTTTCTGTACATGTGTTTTGGTGAGAAGATGGAGGAGGAGAAGGTGAGAGAAATTGTGGACGTTCAGCGTCGGCTTCTGTTGGGTTTGGTCGGAGAGTTTCAGATTCTCAACTTTGGCCCTGCATGGTTTACCAAGATTTTGTTTTATAAGCGGTGGAGTCGCTTCTTCCAGATTCTCCAGCAGCAAATAAACCTCCTGCTGCCTCTGATACAAGCACGGAAGAAGATAAAGGAAAGAAAATGCTCGAGTAACCGAAAAGAAGAAAACAACAACAATCAAGCATTTGTTGATACCTTAATGGATTTAGAACTCCCTGACGAGAAACGAACATTCGATGAAGTAGAAGTGGTGAACCAATGCTCGGATTTCCTCAACTCCGGCGTAGACACAATTGCCATAACACTGCAATGGATGATGGCAAACATAGTGAAACACCCACACATTCAAGACAAACTTGTCACAGAAATTAGACAAATTGTGGGAGACGGAGAAGTTGAAGAGGTGGAAGAGGAGAACCTGCACAGAATGAAGTATTTAAAAGCGGTTGTGCTAGAAGGTCTGCGTCTTCACCCACCGTCGCACTTTTTGCTTCCGCACTCTGTCACTGAGGACGTCATTTTGGACGGGCGTTACGTTGTGACAAAGGACTGCACTGTGAACTTCTCTGTGGCTGAGATGGGGCGGGATCCGAAGGTGTGGGCGGAGCCGATGGAGTTCAAGCCTGAGAGGTTTCTGAGTGATGAGGAGAACTTGTGTGATTTAACTGGTAGTAAGGAGATCAAGATGATGCCGTTTGGGGTAGGGAGGAGGATGTGTCCTGGTTCGAATTTGGCAATGCTGCTTTTGGAGTACTTTGTGGCGAATTTGGTTTGGAAATTCGAGTGGAGAACTGTGGAGGGAGTTGGGGTTGATTTGAGTGAGAAGGAGGAGACGAGTTGGGGGATGAATAATCCATTGCAGGCACATATCACTCCAAGGTTGAAGACATGA

Protein Analysis

521

Amino Acids

59.22

Weight (kDa)

7.17

Isoelectric Point (pI)

43.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 35 - 503 5.2e-84 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017815)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0035991
rosa_laevigata RLG00000033658
rosa_multiflora Rmu_co8295021.1_g000001 Rmu_sc0000084.1_g000043 Rmu_sc0001493.1_g000014
rosa_roxburghii Rroxscaffold_1G00044750
rosa_rugosa Rorug05G0152000
rosa_samantha Rh5AG246000 Rh5BG246400 Rh5CG277200 Rh5DG254900
rosa_wichuraiana Rw5G022600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 720
AasI GACNNNNNNGTC 1 cut(s) 1028
AatII GACGTC 1 cut(s) 1188
Acc36I ACCTGC 2 cut(s) 201, 1098
AccB1I GGYRCC 3 cut(s) 134, 299, 396
AccBSI CCGCTC 1 cut(s) 267
AccI GTMKAC 1 cut(s) 957
AccII CGCG 1 cut(s) 168
AccIII TCCGGA 1 cut(s) 552
AclWI GGATC 3 cut(s) 552, 1253, 1266
AcoI YGGCCR 1 cut(s) 456
AcsI RAATTY 3 cut(s) 1399, 1435, 1448
AcyI GRCGYC 2 cut(s) 397, 1185
AfaI GTAC 3 cut(s) 206, 581, 1424
AfiI CCNNNNNNNGG 6 cut(s) 131, 144, 465, 471, 472, 934
AflIII ACRYGT 1 cut(s) 582
AgsI TTSAA 5 cut(s) 154, 1019, 1070, 1291, 1555
AjnI CCWGG 3 cut(s) 8, 106, 1390
AluBI AGCT 1 cut(s) 427
AluI AGCT 1 cut(s) 427
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 5 cut(s) 223, 424, 444, 1050, 1496
AlwI GGATC 3 cut(s) 552, 1253, 1266
Ama87I CYCGRG 1 cut(s) 817
Aor13HI TCCGGA 1 cut(s) 552
AoxI GGCC 4 cut(s) 450, 456, 474, 688
ApaI GGGCCC 1 cut(s) 478
ApeKI GCWGC 3 cut(s) 754, 772, 1411
ApoI RAATTY 3 cut(s) 1399, 1435, 1448
AspLEI GCGC 2 cut(s) 399, 408
AspS9I GGNCC 5 cut(s) 110, 325, 474, 475, 689
AsuC2I CCSGG 1 cut(s) 466
AsuHPI GGTGA 6 cut(s) 37, 340, 605, 628, 934, 1133
AsuII TTCGAA 1 cut(s) 1397
AvaI CYCGRG 1 cut(s) 817
AvaII GGWCC 2 cut(s) 110, 325
AxyI CCTNAGG 1 cut(s) 321
BaeGI GKGCMC 1 cut(s) 478
BamHI GGATCC 1 cut(s) 1258
BanI GGYRCC 3 cut(s) 134, 299, 396
BanII GRGCYC 3 cut(s) 180, 429, 478
BbsI GAAGAC 3 cut(s) 25, 504, 1130
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 3 cut(s) 759, 766, 1398
BccI CCATC 6 cut(s) 340, 344, 595, 982, 1243, 1276
BceAI ACGGC 4 cut(s) 223, 287, 400, 1348
BciT130I CCWGG 3 cut(s) 10, 108, 1392
BcnI CCSGG 1 cut(s) 466
BcoDI GTCTC 5 cut(s) 223, 424, 444, 1050, 1496
BfaI CTAG 2 cut(s) 479, 1124
BfoI RGCGCY 1 cut(s) 400
BfuAI ACCTGC 2 cut(s) 201, 1098
BisI GCNGC 6 cut(s) 169, 211, 459, 755, 773, 1412
BlsI GCNGC 6 cut(s) 170, 212, 460, 756, 774, 1413
BmcAI AGTACT 1 cut(s) 1424
Bme1390I CCNGG 4 cut(s) 10, 108, 466, 1392
Bme18I GGWCC 2 cut(s) 110, 325
BmeT110I CYCGRG 1 cut(s) 817
BmgT120I GGNCC 5 cut(s) 110, 325, 474, 475, 689
BmrFI CCNGG 4 cut(s) 10, 108, 466, 1392
BmsI GCATC 3 cut(s) 222, 372, 1350
BpiI GAAGAC 3 cut(s) 25, 504, 1130
BplI GAGNNNNNCTC 1 cut(s) 29
BpmI CTGGAG 1 cut(s) 734
Bpu14I TTCGAA 1 cut(s) 1397
BpuEI CTTGAG 1 cut(s) 431
BpuMI CCSGG 1 cut(s) 466
BsaAI YACGTR 2 cut(s) 204, 393
BsaHI GRCGYC 2 cut(s) 397, 1185
BsaJI CCNNGG 3 cut(s) 166, 453, 1547
BsaWI WCCGGW 1 cut(s) 552
BsaXI ACNNNNNCTCC 8 cut(s) 62, 92, 1310, 1340, 1372, 1402, 1464, 1494
Bsc4I CCNNNNNNNGG 6 cut(s) 131, 144, 465, 471, 472, 934
Bse1I ACTGG 1 cut(s) 1342
Bse21I CCTNAGG 1 cut(s) 321
Bse3DI GCAATG 3 cut(s) 986, 1413, 1526
BseAI TCCGGA 1 cut(s) 552
BseBI CCWGG 3 cut(s) 10, 108, 1392
BseDI CCNNGG 3 cut(s) 166, 453, 1547
BseGI GGATG 6 cut(s) 57, 332, 561, 990, 1389, 1521
BseLI CCNNNNNNNGG 6 cut(s) 131, 144, 465, 471, 472, 934
BseMI GCAATG 3 cut(s) 986, 1413, 1526
BseMII CTCAG 6 cut(s) 312, 414, 1170, 1236, 1287, 1298
BseNI ACTGG 1 cut(s) 1342
BseRI GAGGAG 6 cut(s) 620, 623, 1097, 1331, 1393, 1514
BseSI GKGCMC 1 cut(s) 478
BseXI GCAGC 3 cut(s) 759, 766, 1398
BsgI GTGCAG 2 cut(s) 1076, 1207
Bsh1236I CGCG 1 cut(s) 168
Bsh1285I CGRYCG 1 cut(s) 265
BshFI GGCC 4 cut(s) 452, 458, 476, 690
BshNI GGYRCC 3 cut(s) 134, 299, 396
BsiEI CGRYCG 1 cut(s) 265
BsiHKAI GWGCWC 1 cut(s) 429
BsiHKCI CYCGRG 1 cut(s) 817
BsiSI CCGG 4 cut(s) 409, 466, 553, 951
BsiWI CGTACG 1 cut(s) 204
BslFI GGGAC 4 cut(s) 96, 209, 215, 534
BslI CCNNNNNNNGG 6 cut(s) 131, 144, 465, 471, 472, 934
BsmAI GTCTC 5 cut(s) 223, 424, 444, 1050, 1496
BsmBI CGTCTC 5 cut(s) 223, 424, 444, 1050, 1496
BsmFI GGGAC 4 cut(s) 96, 209, 215, 534
BsnI GGCC 4 cut(s) 452, 458, 476, 690
BsoBI CYCGRG 1 cut(s) 817
Bsp119I TTCGAA 1 cut(s) 1397
Bsp120I GGGCCC 1 cut(s) 474
Bsp1286I GDGCHC 3 cut(s) 180, 429, 478
Bsp13I TCCGGA 1 cut(s) 552
Bsp1407I TGTACA 1 cut(s) 579
Bsp143I GATC 4 cut(s) 255, 544, 1258, 1350
BspANI GGCC 4 cut(s) 452, 458, 476, 690
BspCNI CTCAG 6 cut(s) 313, 415, 1171, 1237, 1288, 1299
BspEI TCCGGA 1 cut(s) 552
BspFNI CGCG 1 cut(s) 168
BspHI TCATGA 1 cut(s) 252
BspMI ACCTGC 2 cut(s) 201, 1098
BspPI GGATC 3 cut(s) 552, 1253, 1266
BspQI GCTCTTC 1 cut(s) 155
BspT104I TTCGAA 1 cut(s) 1397
BspT107I GGYRCC 3 cut(s) 134, 299, 396
BsrBI CCGCTC 1 cut(s) 267
BsrDI GCAATG 3 cut(s) 986, 1413, 1526
BsrGI TGTACA 1 cut(s) 579
BsrI ACTGG 1 cut(s) 1342
BssECI CCNNGG 3 cut(s) 166, 453, 1547
BssMI GATC 4 cut(s) 255, 544, 1258, 1350
BssNI GRCGYC 2 cut(s) 397, 1185
BssT1I CCWWGG 2 cut(s) 453, 1547
Bst2UI CCWGG 3 cut(s) 10, 108, 1392
Bst4CI ACNGT 4 cut(s) 299, 1149, 1228, 1465
Bst6I CTCTTC 4 cut(s) 68, 155, 1065, 1074
BstACI GRCGYC 2 cut(s) 397, 1185
BstAPI GCANNNNNTGC 1 cut(s) 1159
BstAUI TGTACA 1 cut(s) 579
BstBAI YACGTR 2 cut(s) 204, 393
BstBI TTCGAA 1 cut(s) 1397
BstC8I GCNNGC 4 cut(s) 198, 379, 493, 1533
BstDEI CTNAG 6 cut(s) 321, 423, 1179, 1245, 1296, 1307
BstDSI CCRYGG 1 cut(s) 166
BstF5I GGATG 6 cut(s) 57, 332, 561, 990, 1389, 1521
BstFNI CGCG 1 cut(s) 168
BstH2I RGCGCY 1 cut(s) 400
BstHHI GCGC 2 cut(s) 399, 408
BstKTI GATC 4 cut(s) 258, 547, 1261, 1353
BstMAI GTCTC 5 cut(s) 223, 424, 444, 1050, 1496
BstMBI GATC 4 cut(s) 255, 544, 1258, 1350
BstMCI CGRYCG 1 cut(s) 265
BstMWI GCNNNNNNNGC 8 cut(s) 281, 308, 360, 403, 405, 458, 492, 1159
BstNI CCWGG 3 cut(s) 10, 108, 1392
BstNSI RCATGY 2 cut(s) 200, 586
BstSCI CCNGG 4 cut(s) 8, 106, 464, 1390
BstSLI GKGCMC 1 cut(s) 478
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 3 cut(s) 759, 766, 1398
BstV2I GAAGAC 3 cut(s) 25, 504, 1130
BstX2I RGATCY 1 cut(s) 1258
BstYI RGATCY 1 cut(s) 1258
Bsu36I CCTNAGG 1 cut(s) 321
BsuRI GGCC 4 cut(s) 452, 458, 476, 690
BtgI CCRYGG 1 cut(s) 166
BtgZI GCGATG 1 cut(s) 352
BtsCI GGATG 6 cut(s) 57, 332, 561, 990, 1389, 1521
BtsI GCAGTG 1 cut(s) 974
BtsIMutI CAGTG 3 cut(s) 974, 1176, 1224
BveI ACCTGC 2 cut(s) 201, 1098
Cac8I GCNNGC 4 cut(s) 198, 379, 493, 1533
CciI TCATGA 1 cut(s) 252
CfoI GCGC 2 cut(s) 399, 408
Cfr13I GGNCC 5 cut(s) 110, 325, 474, 475, 689
Cfr42I CCGCGG 1 cut(s) 169
CseI GACGC 2 cut(s) 629, 1124
Csp6I GTAC 3 cut(s) 205, 580, 1423
CviAII CATG 8 cut(s) 197, 253, 430, 529, 562, 583, 696, 1560
CviQI GTAC 3 cut(s) 205, 580, 1423
DdeI CTNAG 6 cut(s) 321, 423, 1179, 1245, 1296, 1307
DinI GGCGCC 1 cut(s) 398
DpnI GATC 4 cut(s) 257, 546, 1260, 1352
DpnII GATC 4 cut(s) 255, 544, 1258, 1350
DraI TTTAAA 1 cut(s) 1110
DrdI GACNNNNNNGTC 1 cut(s) 1028
DseDI GACNNNNNNGTC 1 cut(s) 1028
EaeI YGGCCR 1 cut(s) 456
Eam1104I CTCTTC 4 cut(s) 68, 155, 1065, 1074
EarI CTCTTC 4 cut(s) 68, 155, 1065, 1074
EciI GGCGGA 1 cut(s) 1289
Ecl136II GAGCTC 1 cut(s) 427
Eco130I CCWWGG 2 cut(s) 453, 1547
Eco147I AGGCCT 1 cut(s) 452
Eco24I GRGCYC 3 cut(s) 180, 429, 478
Eco47I GGWCC 2 cut(s) 110, 325
Eco53kI GAGCTC 1 cut(s) 427
Eco81I CCTNAGG 1 cut(s) 321
Eco88I CYCGRG 1 cut(s) 817
EcoICRI GAGCTC 1 cut(s) 427
EcoO109I RGGNCCY 1 cut(s) 475
EcoRII CCWGG 3 cut(s) 8, 106, 1390
EcoT14I CCWWGG 2 cut(s) 453, 1547
EcoT38I GRGCYC 3 cut(s) 180, 429, 478
EgeI GGCGCC 1 cut(s) 398
EheI GGCGCC 1 cut(s) 398
ErhI CCWWGG 2 cut(s) 453, 1547
Esp3I CGTCTC 5 cut(s) 223, 424, 444, 1050, 1496
FaeI CATG 8 cut(s) 200, 256, 433, 532, 565, 586, 699, 1563
FalI AAGNNNNNCTT 2 cut(s) 1011, 1043
FaqI GGGAC 4 cut(s) 96, 209, 215, 534
FatI CATG 8 cut(s) 196, 252, 429, 528, 561, 582, 695, 1559
FauI CCCGC 2 cut(s) 173, 1248
FauNDI CATATG 1 cut(s) 143
FblI GTMKAC 1 cut(s) 957
Fnu4HI GCNGC 6 cut(s) 169, 211, 459, 755, 773, 1412
FokI GGATG 6 cut(s) 44, 319, 568, 997, 1396, 1528
FriOI GRGCYC 3 cut(s) 180, 429, 478
Fsp4HI GCNGC 6 cut(s) 169, 211, 459, 755, 773, 1412
FspBI CTAG 2 cut(s) 479, 1124
GlaI GCGC 2 cut(s) 398, 407
GluI GCNGC 6 cut(s) 169, 211, 459, 755, 773, 1412
GsuI CTGGAG 1 cut(s) 734
HaeII RGCGCY 1 cut(s) 400
HaeIII GGCC 4 cut(s) 452, 458, 476, 690
HapII CCGG 4 cut(s) 409, 466, 553, 951
HgaI GACGC 2 cut(s) 629, 1124
HhaI GCGC 2 cut(s) 399, 408
Hin1I GRCGYC 2 cut(s) 397, 1185
Hin1II CATG 8 cut(s) 200, 256, 433, 532, 565, 586, 699, 1563
Hin6I GCGC 2 cut(s) 397, 406
HinP1I GCGC 2 cut(s) 397, 406
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 3 cut(s) 676, 730, 745
HpaII CCGG 4 cut(s) 409, 466, 553, 951
HphI GGTGA 6 cut(s) 37, 340, 605, 628, 934, 1133
Hpy166II GTNNAC 6 cut(s) 292, 631, 702, 925, 958, 1231
Hpy188I TCNGA 7 cut(s) 424, 665, 675, 780, 937, 1263, 1308
Hpy188III TCNNGA 6 cut(s) 253, 519, 553, 742, 1019, 1354
Hpy8I GTNNAC 6 cut(s) 292, 631, 702, 925, 958, 1231
Hpy99I CGWCG 5 cut(s) 131, 134, 511, 645, 1153
HpyAV CCTTC 7 cut(s) 34, 148, 244, 607, 1121, 1258, 1489
HpyCH4III ACNGT 4 cut(s) 299, 1149, 1228, 1465
HpyCH4IV ACGT 7 cut(s) 203, 392, 506, 523, 633, 1185, 1206
HpyCH4V TGCA 7 cut(s) 196, 503, 695, 979, 1093, 1224, 1531
HpyF10VI GCNNNNNNNGC 8 cut(s) 281, 308, 360, 403, 405, 458, 492, 1159
HpyF3I CTNAG 6 cut(s) 321, 423, 1179, 1245, 1296, 1307
HpySE526I ACGT 7 cut(s) 203, 392, 506, 523, 633, 1185, 1206
Hsp92I GRCGYC 2 cut(s) 397, 1185
Hsp92II CATG 8 cut(s) 200, 256, 433, 532, 565, 586, 699, 1563
HspAI GCGC 2 cut(s) 397, 406
KasI GGCGCC 1 cut(s) 396
Kpn2I TCCGGA 1 cut(s) 552
KspI CCGCGG 1 cut(s) 169
Kzo9I GATC 4 cut(s) 255, 544, 1258, 1350
LguI GCTCTTC 1 cut(s) 155
LmnI GCTCC 4 cut(s) 5, 18, 272, 1276
Lsp1109I GCAGC 3 cut(s) 759, 766, 1398
LweI GCATC 3 cut(s) 222, 372, 1350
MaeI CTAG 2 cut(s) 479, 1124
MaeII ACGT 7 cut(s) 203, 392, 506, 523, 633, 1185, 1206
MaeIII GTNAC 7 cut(s) 188, 524, 821, 1030, 1174, 1202, 1210
MalI GATC 4 cut(s) 257, 546, 1260, 1352
MbiI CCGCTC 1 cut(s) 267
MboI GATC 4 cut(s) 255, 544, 1258, 1350
MfeI CAATTG 1 cut(s) 963
MflI RGATCY 1 cut(s) 1258
MhlI GDGCHC 3 cut(s) 180, 429, 478
MluCI AATT 7 cut(s) 624, 963, 1038, 1047, 1399, 1435, 1448
Mly113I GGCGCC 1 cut(s) 397
MlyI GAGTC 1 cut(s) 739
MmeI TCCRAC 2 cut(s) 352, 643
MroI TCCGGA 1 cut(s) 552
MseI TTAA 3 cut(s) 869, 1109, 1334
MslI CAYNNNNRTG 1 cut(s) 979
MspA1I CMGCKG 2 cut(s) 168, 377
MspI CCGG 4 cut(s) 409, 466, 553, 951
MspR9I CCNGG 4 cut(s) 10, 108, 466, 1392
MunI CAATTG 1 cut(s) 963
MvaI CCWGG 3 cut(s) 10, 108, 1392
MvnI CGCG 1 cut(s) 168
MwoI GCNNNNNNNGC 8 cut(s) 281, 308, 360, 403, 405, 458, 492, 1159
NarI GGCGCC 1 cut(s) 397
NciI CCSGG 1 cut(s) 466
NdeI CATATG 1 cut(s) 143
NdeII GATC 4 cut(s) 255, 544, 1258, 1350
NlaIII CATG 8 cut(s) 200, 256, 433, 532, 565, 586, 699, 1563
NmeAIII GCCGAG 1 cut(s) 421
NmuCI GTSAC 4 cut(s) 188, 1030, 1174, 1210
NspI RCATGY 2 cut(s) 200, 586
NspV TTCGAA 1 cut(s) 1397
PaeI GCATGC 1 cut(s) 200
PaeR7I CTCGAG 1 cut(s) 817
PagI TCATGA 1 cut(s) 252
PceI AGGCCT 1 cut(s) 452
PciI ACATGT 1 cut(s) 582
PciSI GCTCTTC 1 cut(s) 155
PcsI WCGNNNNNNNCGW 2 cut(s) 213, 1203
PfeI GAWTC 2 cut(s) 676, 745
Pfl23II CGTACG 1 cut(s) 204
PfoI TCCNGGA 1 cut(s) 464
PkrI GCNGC 6 cut(s) 170, 212, 460, 756, 774, 1413
PleI GAGTC 1 cut(s) 738
PluTI GGCGCC 1 cut(s) 400
PpsI GAGTC 1 cut(s) 738
Ppu21I YACGTR 2 cut(s) 204, 393
PscI ACATGT 1 cut(s) 582
PsiI TTATAA 1 cut(s) 720
Psp124BI GAGCTC 1 cut(s) 429
Psp6I CCWGG 3 cut(s) 8, 106, 1390
PspGI CCWGG 3 cut(s) 8, 106, 1390
PspLI CGTACG 1 cut(s) 204
PspOMI GGGCCC 1 cut(s) 474
PspPI GGNCC 5 cut(s) 110, 325, 474, 475, 689
PspXI VCTCGAGB 1 cut(s) 817
PsuI RGATCY 1 cut(s) 1258
RsaI GTAC 3 cut(s) 206, 581, 1424
RsaNI GTAC 3 cut(s) 205, 580, 1423
RseI CAYNNNNRTG 1 cut(s) 979
SacI GAGCTC 1 cut(s) 429
SacII CCGCGG 1 cut(s) 169
SapI GCTCTTC 1 cut(s) 155
SaqAI TTAA 3 cut(s) 869, 1109, 1334
SatI GCNGC 6 cut(s) 169, 211, 459, 755, 773, 1412
Sau3AI GATC 4 cut(s) 255, 544, 1258, 1350
Sau96I GGNCC 5 cut(s) 110, 325, 474, 475, 689
ScaI AGTACT 1 cut(s) 1424
SchI GAGTC 1 cut(s) 739
ScrFI CCNGG 4 cut(s) 10, 108, 466, 1392
SduI GDGCHC 3 cut(s) 180, 429, 478
SfaNI GCATC 3 cut(s) 222, 372, 1350
SfoI GGCGCC 1 cut(s) 398
Sfr274I CTCGAG 1 cut(s) 817
Sfr303I CCGCGG 1 cut(s) 169
SfuI TTCGAA 1 cut(s) 1397
SgrBI CCGCGG 1 cut(s) 169
SinI GGWCC 2 cut(s) 110, 325
SlaI CTCGAG 1 cut(s) 817
SmiMI CAYNNNNRTG 1 cut(s) 979
SmlI CTYRAG 2 cut(s) 446, 817
SmoI CTYRAG 2 cut(s) 446, 817
SphI GCATGC 1 cut(s) 200
Sse9I AATT 7 cut(s) 624, 963, 1038, 1047, 1399, 1435, 1448
SseBI AGGCCT 1 cut(s) 452
SspDI GGCGCC 1 cut(s) 396
SspMI CTAG 2 cut(s) 479, 1124
SstI GAGCTC 1 cut(s) 429
StuI AGGCCT 1 cut(s) 452
StyD4I CCNGG 4 cut(s) 8, 106, 464, 1390
StyI CCWWGG 2 cut(s) 453, 1547
TaaI ACNGT 4 cut(s) 299, 1149, 1228, 1465
TaiI ACGT 7 cut(s) 206, 395, 509, 526, 636, 1188, 1209
TaqI TCGA 4 cut(s) 818, 906, 1397, 1452
TasI AATT 7 cut(s) 624, 963, 1038, 1047, 1399, 1435, 1448
TatI WGTACW 2 cut(s) 579, 1422
TauI GCSGC 3 cut(s) 171, 213, 461
TfiI GAWTC 2 cut(s) 676, 745
Tru1I TTAA 3 cut(s) 869, 1109, 1334
Tru9I TTAA 3 cut(s) 869, 1109, 1334
TscAI CASTG 3 cut(s) 981, 1183, 1231
TseFI GTSAC 4 cut(s) 188, 1030, 1174, 1210
TseI GCWGC 3 cut(s) 754, 772, 1411
Tsp45I GTSAC 4 cut(s) 188, 1030, 1174, 1210
TspDTI ATGAA 7 cut(s) 46, 130, 334, 504, 924, 1115, 1532
TspGWI ACGGA 5 cut(s) 19, 176, 426, 805, 1074
TspRI CASTG 3 cut(s) 981, 1183, 1231
VpaK11BI GGWCC 2 cut(s) 110, 325
XapI RAATTY 3 cut(s) 1399, 1435, 1448
XceI RCATGY 2 cut(s) 200, 586
XhoI CTCGAG 1 cut(s) 817
XmiI GTMKAC 1 cut(s) 957
XspI CTAG 2 cut(s) 479, 1124
ZraI GACGTC 1 cut(s) 1186
ZrmI AGTACT 1 cut(s) 1424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.