Rroxscaffold_1G00044750

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0017815
Physical Location & Seq
Forward (+)
63770933 .. 63772825
1893 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00044750.1

Sequence Viewer

Length: 1233 bp
ATGGGAGCCTGGCTCCTTATACCCTTCTCCGTCTTCCTCACTTTCACCCTAAAGTTCATCCTCTTCCCCTACAAACCCTCCCACAAACTCAACGCCCCACTCCCTCCTGGTCCCACCACCATACCCGTCGTCGGCACCTTCATATGGCTTTTCAAACTCGCCTCCCGCGGCATCGAGCCCGTCCTCCGTGACCTACATGCCACGTACGGCGGCATCGTCTCTGTCCCCGTGCCCTTCTCTTACCCCGTCATCATGATCAACGACCGCTCCCTCGCCCACCAAGCCCTTGTCAACAACGGTGCCTGTTTTGCCGACCGCCCTGAGGTCCAACCATCCATCAAGTTCATCACCAGCAACCAACGCATCATCGCCACCGCTGGCTACGGCTCTACGTGGCGCCTCCTGCGCCGGAACCTCGTCTCTGAGCTCATGGTTCCGTCTCGGCTCAAGGCCTTGGCCGGTTCCCGGAAGTGGGCCCTAGACAGCCTCACCAGCCGTCTTGCAAGTTCGTCGTCATCGGGACGTAACGTGGTGGGAGTTTTGGATCATTTCCGGATGTCCATGACGGGTTTGTTTCTGTACATGTGTTTTGGTGAGAAGATGGAGGAGGAGAAGGTGAGAGAAATTGTGGACGTTCAGCGTCGGCTTCTGTTGGGTTTGGTCGGAGAGTTTCAGATTCTCAACTTTGGGCCTGCATGGTTTACCAAGATTTTGTTTTATAAGCGGTGGAGTCGCTTCTTCCAGATTCTCCAGCAGCAAATAAACCTCCTGCTGCCTCTGATACAAGCACGGAAGAAGATAAAGGAAAGAAAATGCTCGAGTAACCGAAAAGAAGATAACAACAACAATCAAGCATTTGTTGATACCTTAATGGATTTAGAACTCCCCGACGAGAAACGAACATTCGATGAAGTAGAAGTGGTGAACCAATGCTCGGATTTCCTCAACTCCGGCGTAGACACAATTGCCATAACACTGCAATGGATGATGGCAAACATAGTGAAACACCCACACATTCAAGACAAACTTGTCACAGAAATTAGACAAATTGTGGGAGATGGAGAAGTTGAAGAGGTGGAAGAGGAGAACCTGCACAGAATGAAGTATTTAAAAGCGGTTGTGCTAGAAGGTCTTCGTCTTCACCCGCCGTCGCACTTTTTTTGCTTCCGCACTCTGTCACTGAGGACGTCATTTTGGACGGGCGTTACGTTGTGCCAAAGGACTGCACTGTGA

Protein Analysis

410

Amino Acids

46.86

Weight (kDa)

9.4

Isoelectric Point (pI)

45.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 35 - 393 1.2e-48 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017815)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0035991
rosa_laevigata RLG00000033658
rosa_multiflora Rmu_co8295021.1_g000001 Rmu_sc0000084.1_g000043 Rmu_sc0001493.1_g000014
rosa_roxburghii Rroxscaffold_1G00044750
rosa_rugosa Rorug05G0152000
rosa_samantha Rh5AG246000 Rh5BG246400 Rh5CG277200 Rh5DG254900
rosa_wichuraiana Rw5G022600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 720
AasI GACNNNNNNGTC 1 cut(s) 1028
AatII GACGTC 1 cut(s) 1190
Acc36I ACCTGC 1 cut(s) 1098
AccB1I GGYRCC 3 cut(s) 134, 299, 396
AccBSI CCGCTC 1 cut(s) 267
AccI GTMKAC 1 cut(s) 957
AccII CGCG 1 cut(s) 168
AccIII TCCGGA 1 cut(s) 552
AclWI GGATC 1 cut(s) 552
AcoI YGGCCR 1 cut(s) 456
AcyI GRCGYC 2 cut(s) 397, 1187
AfaI GTAC 2 cut(s) 206, 581
AfiI CCNNNNNNNGG 7 cut(s) 131, 144, 322, 465, 471, 472, 934
AflIII ACRYGT 1 cut(s) 582
AgsI TTSAA 3 cut(s) 154, 1019, 1070
AjnI CCWGG 2 cut(s) 8, 106
AluBI AGCT 1 cut(s) 427
AluI AGCT 1 cut(s) 427
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 3 cut(s) 223, 424, 444
AlwI GGATC 1 cut(s) 552
Ama87I CYCGRG 1 cut(s) 817
Aor13HI TCCGGA 1 cut(s) 552
AoxI GGCC 4 cut(s) 450, 456, 474, 689
ApaI GGGCCC 1 cut(s) 478
ApeKI GCWGC 2 cut(s) 754, 772
Asp700I GAANNNNTTC 1 cut(s) 1131
AspLEI GCGC 2 cut(s) 399, 408
AspS9I GGNCC 5 cut(s) 110, 325, 474, 475, 689
AsuC2I CCSGG 1 cut(s) 466
AsuHPI GGTGA 7 cut(s) 37, 340, 481, 605, 628, 934, 1133
AvaI CYCGRG 1 cut(s) 817
AvaII GGWCC 2 cut(s) 110, 325
AxyI CCTNAGG 1 cut(s) 321
BaeGI GKGCMC 2 cut(s) 234, 478
BanI GGYRCC 3 cut(s) 134, 299, 396
BanII GRGCYC 3 cut(s) 180, 429, 478
BbsI GAAGAC 3 cut(s) 25, 1124, 1130
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 2 cut(s) 759, 766
BccI CCATC 5 cut(s) 340, 344, 595, 982, 1052
BceAI ACGGC 4 cut(s) 223, 400, 480, 1132
BcgI CGANNNNNNTGC 2 cut(s) 492, 526
BciT130I CCWGG 2 cut(s) 10, 108
BclI TGATCA 1 cut(s) 255
BcnI CCSGG 1 cut(s) 466
BcoDI GTCTC 3 cut(s) 223, 424, 444
BfaI CTAG 2 cut(s) 479, 1124
BfoI RGCGCY 1 cut(s) 400
BfuAI ACCTGC 1 cut(s) 1098
BisI GCNGC 4 cut(s) 169, 211, 755, 773
BlsI GCNGC 4 cut(s) 170, 212, 756, 774
Bme1390I CCNGG 3 cut(s) 10, 108, 466
Bme18I GGWCC 2 cut(s) 110, 325
BmeT110I CYCGRG 1 cut(s) 817
BmgT120I GGNCC 5 cut(s) 110, 325, 474, 475, 689
BmrFI CCNGG 3 cut(s) 10, 108, 466
BmsI GCATC 3 cut(s) 180, 222, 372
BpiI GAAGAC 3 cut(s) 25, 1124, 1130
BplI GAGNNNNNCTC 1 cut(s) 29
BpmI CTGGAG 1 cut(s) 734
BpuEI CTTGAG 1 cut(s) 431
BpuMI CCSGG 1 cut(s) 466
BsaAI YACGTR 2 cut(s) 204, 393
BsaHI GRCGYC 2 cut(s) 397, 1187
BsaJI CCNNGG 2 cut(s) 166, 453
BsaWI WCCGGW 1 cut(s) 552
BsaXI ACNNNNNCTCC 4 cut(s) 62, 92, 251, 281
Bsc4I CCNNNNNNNGG 7 cut(s) 131, 144, 322, 465, 471, 472, 934
Bse118I RCCGGY 1 cut(s) 458
Bse21I CCTNAGG 1 cut(s) 321
Bse3DI GCAATG 1 cut(s) 986
BseAI TCCGGA 1 cut(s) 552
BseBI CCWGG 2 cut(s) 10, 108
BseDI CCNNGG 2 cut(s) 166, 453
BseGI GGATG 4 cut(s) 57, 332, 561, 990
BseLI CCNNNNNNNGG 7 cut(s) 131, 144, 322, 465, 471, 472, 934
BseMI GCAATG 1 cut(s) 986
BseMII CTCAG 3 cut(s) 312, 414, 1172
BseRI GAGGAG 3 cut(s) 620, 623, 1097
BseSI GKGCMC 2 cut(s) 234, 478
BseXI GCAGC 2 cut(s) 759, 766
BsgI GTGCAG 2 cut(s) 1076, 1209
Bsh1236I CGCG 1 cut(s) 168
Bsh1285I CGRYCG 2 cut(s) 265, 316
BshFI GGCC 4 cut(s) 452, 458, 476, 691
BshNI GGYRCC 3 cut(s) 134, 299, 396
BsiEI CGRYCG 2 cut(s) 265, 316
BsiHKAI GWGCWC 1 cut(s) 429
BsiHKCI CYCGRG 1 cut(s) 817
BsiSI CCGG 5 cut(s) 409, 459, 466, 553, 951
BsiWI CGTACG 1 cut(s) 204
BslFI GGGAC 3 cut(s) 96, 209, 534
BslI CCNNNNNNNGG 7 cut(s) 131, 144, 322, 465, 471, 472, 934
BsmAI GTCTC 3 cut(s) 223, 424, 444
BsmBI CGTCTC 3 cut(s) 223, 424, 444
BsmFI GGGAC 3 cut(s) 96, 209, 534
BsnI GGCC 4 cut(s) 452, 458, 476, 691
BsoBI CYCGRG 1 cut(s) 817
Bsp120I GGGCCC 1 cut(s) 474
Bsp1286I GDGCHC 4 cut(s) 180, 234, 429, 478
Bsp13I TCCGGA 1 cut(s) 552
Bsp1407I TGTACA 1 cut(s) 579
Bsp143I GATC 2 cut(s) 255, 544
BspANI GGCC 4 cut(s) 452, 458, 476, 691
BspCNI CTCAG 3 cut(s) 313, 415, 1173
BspEI TCCGGA 1 cut(s) 552
BspFNI CGCG 1 cut(s) 168
BspHI TCATGA 1 cut(s) 252
BspMI ACCTGC 1 cut(s) 1098
BspPI GGATC 1 cut(s) 552
BspT107I GGYRCC 3 cut(s) 134, 299, 396
BsrBI CCGCTC 1 cut(s) 267
BsrDI GCAATG 1 cut(s) 986
BsrFI RCCGGY 1 cut(s) 458
BsrGI TGTACA 1 cut(s) 579
BssAI RCCGGY 1 cut(s) 458
BssECI CCNNGG 2 cut(s) 166, 453
BssMI GATC 2 cut(s) 255, 544
BssNI GRCGYC 2 cut(s) 397, 1187
BssT1I CCWWGG 1 cut(s) 453
Bst2UI CCWGG 2 cut(s) 10, 108
Bst4CI ACNGT 2 cut(s) 299, 1230
Bst6I CTCTTC 3 cut(s) 68, 1065, 1074
BstACI GRCGYC 2 cut(s) 397, 1187
BstAUI TGTACA 1 cut(s) 579
BstBAI YACGTR 2 cut(s) 204, 393
BstC8I GCNNGC 2 cut(s) 379, 693
BstDEI CTNAG 3 cut(s) 321, 423, 1181
BstDSI CCRYGG 1 cut(s) 166
BstF5I GGATG 4 cut(s) 57, 332, 561, 990
BstFNI CGCG 1 cut(s) 168
BstH2I RGCGCY 1 cut(s) 400
BstHHI GCGC 2 cut(s) 399, 408
BstKTI GATC 2 cut(s) 258, 547
BstMAI GTCTC 3 cut(s) 223, 424, 444
BstMBI GATC 2 cut(s) 255, 544
BstMCI CGRYCG 2 cut(s) 265, 316
BstMWI GCNNNNNNNGC 6 cut(s) 281, 308, 360, 403, 405, 492
BstNI CCWGG 2 cut(s) 10, 108
BstNSI RCATGY 2 cut(s) 200, 586
BstSCI CCNGG 3 cut(s) 8, 106, 464
BstSLI GKGCMC 2 cut(s) 234, 478
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 2 cut(s) 759, 766
BstV2I GAAGAC 3 cut(s) 25, 1124, 1130
Bsu36I CCTNAGG 1 cut(s) 321
BsuRI GGCC 4 cut(s) 452, 458, 476, 691
BtgI CCRYGG 1 cut(s) 166
BtgZI GCGATG 1 cut(s) 352
BtsCI GGATG 4 cut(s) 57, 332, 561, 990
BtsI GCAGTG 1 cut(s) 974
BtsIMutI CAGTG 3 cut(s) 974, 1178, 1226
BveI ACCTGC 1 cut(s) 1098
Cac8I GCNNGC 2 cut(s) 379, 693
CciI TCATGA 1 cut(s) 252
CfoI GCGC 2 cut(s) 399, 408
Cfr10I RCCGGY 1 cut(s) 458
Cfr13I GGNCC 5 cut(s) 110, 325, 474, 475, 689
Cfr42I CCGCGG 1 cut(s) 169
CseI GACGC 1 cut(s) 629
Csp6I GTAC 2 cut(s) 205, 580
CviAII CATG 6 cut(s) 197, 253, 430, 562, 583, 696
CviQI GTAC 2 cut(s) 205, 580
DdeI CTNAG 3 cut(s) 321, 423, 1181
DinI GGCGCC 1 cut(s) 398
DpnI GATC 2 cut(s) 257, 546
DpnII GATC 2 cut(s) 255, 544
DraI TTTAAA 1 cut(s) 1110
DrdI GACNNNNNNGTC 1 cut(s) 1028
DseDI GACNNNNNNGTC 1 cut(s) 1028
EaeI YGGCCR 1 cut(s) 456
Eam1104I CTCTTC 3 cut(s) 68, 1065, 1074
EarI CTCTTC 3 cut(s) 68, 1065, 1074
Ecl136II GAGCTC 1 cut(s) 427
Eco130I CCWWGG 1 cut(s) 453
Eco147I AGGCCT 1 cut(s) 452
Eco24I GRGCYC 3 cut(s) 180, 429, 478
Eco47I GGWCC 2 cut(s) 110, 325
Eco53kI GAGCTC 1 cut(s) 427
Eco81I CCTNAGG 1 cut(s) 321
Eco88I CYCGRG 1 cut(s) 817
EcoICRI GAGCTC 1 cut(s) 427
EcoO109I RGGNCCY 1 cut(s) 475
EcoRII CCWGG 2 cut(s) 8, 106
EcoT14I CCWWGG 1 cut(s) 453
EcoT38I GRGCYC 3 cut(s) 180, 429, 478
EgeI GGCGCC 1 cut(s) 398
EheI GGCGCC 1 cut(s) 398
ErhI CCWWGG 1 cut(s) 453
Esp3I CGTCTC 3 cut(s) 223, 424, 444
FaeI CATG 6 cut(s) 200, 256, 433, 565, 586, 699
FalI AAGNNNNNCTT 2 cut(s) 1011, 1043
FaqI GGGAC 3 cut(s) 96, 209, 534
FatI CATG 6 cut(s) 196, 252, 429, 561, 582, 695
FauI CCCGC 2 cut(s) 173, 1152
FauNDI CATATG 1 cut(s) 143
FbaI TGATCA 1 cut(s) 255
FblI GTMKAC 1 cut(s) 957
Fnu4HI GCNGC 4 cut(s) 169, 211, 755, 773
FokI GGATG 4 cut(s) 44, 319, 568, 997
FriOI GRGCYC 3 cut(s) 180, 429, 478
Fsp4HI GCNGC 4 cut(s) 169, 211, 755, 773
FspBI CTAG 2 cut(s) 479, 1124
GlaI GCGC 2 cut(s) 398, 407
GluI GCNGC 4 cut(s) 169, 211, 755, 773
GsuI CTGGAG 1 cut(s) 734
HaeII RGCGCY 1 cut(s) 400
HaeIII GGCC 4 cut(s) 452, 458, 476, 691
HapII CCGG 5 cut(s) 409, 459, 466, 553, 951
HgaI GACGC 1 cut(s) 629
HhaI GCGC 2 cut(s) 399, 408
Hin1I GRCGYC 2 cut(s) 397, 1187
Hin1II CATG 6 cut(s) 200, 256, 433, 565, 586, 699
Hin6I GCGC 2 cut(s) 397, 406
HinP1I GCGC 2 cut(s) 397, 406
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 3 cut(s) 676, 730, 745
HpaII CCGG 5 cut(s) 409, 459, 466, 553, 951
HphI GGTGA 7 cut(s) 37, 340, 481, 605, 628, 934, 1133
Hpy166II GTNNAC 5 cut(s) 292, 631, 702, 925, 958
Hpy188I TCNGA 5 cut(s) 424, 665, 675, 780, 937
Hpy188III TCNNGA 5 cut(s) 253, 519, 553, 742, 1019
Hpy8I GTNNAC 5 cut(s) 292, 631, 702, 925, 958
Hpy99I CGWCG 6 cut(s) 131, 134, 514, 645, 893, 1153
HpyAV CCTTC 5 cut(s) 34, 148, 244, 607, 1121
HpyCH4III ACNGT 2 cut(s) 299, 1230
HpyCH4IV ACGT 7 cut(s) 203, 392, 523, 528, 633, 1187, 1208
HpyCH4V TGCA 5 cut(s) 503, 695, 979, 1093, 1226
HpyF10VI GCNNNNNNNGC 6 cut(s) 281, 308, 360, 403, 405, 492
HpyF3I CTNAG 3 cut(s) 321, 423, 1181
HpySE526I ACGT 7 cut(s) 203, 392, 523, 528, 633, 1187, 1208
Hsp92I GRCGYC 2 cut(s) 397, 1187
Hsp92II CATG 6 cut(s) 200, 256, 433, 565, 586, 699
HspAI GCGC 2 cut(s) 397, 406
KasI GGCGCC 1 cut(s) 396
Kpn2I TCCGGA 1 cut(s) 552
Ksp22I TGATCA 1 cut(s) 255
KspI CCGCGG 1 cut(s) 169
Kzo9I GATC 2 cut(s) 255, 544
LmnI GCTCC 3 cut(s) 5, 18, 272
Lsp1109I GCAGC 2 cut(s) 759, 766
LweI GCATC 3 cut(s) 180, 222, 372
MaeI CTAG 2 cut(s) 479, 1124
MaeII ACGT 7 cut(s) 203, 392, 523, 528, 633, 1187, 1208
MaeIII GTNAC 6 cut(s) 188, 524, 821, 1030, 1176, 1204
MalI GATC 2 cut(s) 257, 546
MbiI CCGCTC 1 cut(s) 267
MboI GATC 2 cut(s) 255, 544
MfeI CAATTG 1 cut(s) 963
MhlI GDGCHC 4 cut(s) 180, 234, 429, 478
MluCI AATT 4 cut(s) 624, 963, 1038, 1047
Mly113I GGCGCC 1 cut(s) 397
MlyI GAGTC 1 cut(s) 739
MmeI TCCRAC 2 cut(s) 352, 643
MroI TCCGGA 1 cut(s) 552
MroXI GAANNNNTTC 1 cut(s) 1131
MseI TTAA 2 cut(s) 869, 1109
MslI CAYNNNNRTG 1 cut(s) 979
MspA1I CMGCKG 2 cut(s) 168, 377
MspI CCGG 5 cut(s) 409, 459, 466, 553, 951
MspR9I CCNGG 3 cut(s) 10, 108, 466
MunI CAATTG 1 cut(s) 963
MvaI CCWGG 2 cut(s) 10, 108
MvnI CGCG 1 cut(s) 168
MwoI GCNNNNNNNGC 6 cut(s) 281, 308, 360, 403, 405, 492
NarI GGCGCC 1 cut(s) 397
NciI CCSGG 1 cut(s) 466
NdeI CATATG 1 cut(s) 143
NdeII GATC 2 cut(s) 255, 544
NlaIII CATG 6 cut(s) 200, 256, 433, 565, 586, 699
NmeAIII GCCGAG 1 cut(s) 421
NmuCI GTSAC 3 cut(s) 188, 1030, 1176
NspI RCATGY 2 cut(s) 200, 586
PaeR7I CTCGAG 1 cut(s) 817
PagI TCATGA 1 cut(s) 252
PceI AGGCCT 1 cut(s) 452
PciI ACATGT 1 cut(s) 582
PcsI WCGNNNNNNNCGW 2 cut(s) 213, 1205
PdmI GAANNNNTTC 1 cut(s) 1131
PfeI GAWTC 2 cut(s) 676, 745
Pfl23II CGTACG 1 cut(s) 204
PfoI TCCNGGA 1 cut(s) 464
PkrI GCNGC 4 cut(s) 170, 212, 756, 774
PleI GAGTC 1 cut(s) 738
PluTI GGCGCC 1 cut(s) 400
PpsI GAGTC 1 cut(s) 738
Ppu21I YACGTR 2 cut(s) 204, 393
PscI ACATGT 1 cut(s) 582
PsiI TTATAA 1 cut(s) 720
Psp124BI GAGCTC 1 cut(s) 429
Psp6I CCWGG 2 cut(s) 8, 106
PspGI CCWGG 2 cut(s) 8, 106
PspLI CGTACG 1 cut(s) 204
PspOMI GGGCCC 1 cut(s) 474
PspPI GGNCC 5 cut(s) 110, 325, 474, 475, 689
PspXI VCTCGAGB 1 cut(s) 817
RsaI GTAC 2 cut(s) 206, 581
RsaNI GTAC 2 cut(s) 205, 580
RseI CAYNNNNRTG 1 cut(s) 979
SacI GAGCTC 1 cut(s) 429
SacII CCGCGG 1 cut(s) 169
SaqAI TTAA 2 cut(s) 869, 1109
SatI GCNGC 4 cut(s) 169, 211, 755, 773
Sau3AI GATC 2 cut(s) 255, 544
Sau96I GGNCC 5 cut(s) 110, 325, 474, 475, 689
SchI GAGTC 1 cut(s) 739
ScrFI CCNGG 3 cut(s) 10, 108, 466
SduI GDGCHC 4 cut(s) 180, 234, 429, 478
SfaNI GCATC 3 cut(s) 180, 222, 372
SfoI GGCGCC 1 cut(s) 398
Sfr274I CTCGAG 1 cut(s) 817
Sfr303I CCGCGG 1 cut(s) 169
SgrBI CCGCGG 1 cut(s) 169
SinI GGWCC 2 cut(s) 110, 325
SlaI CTCGAG 1 cut(s) 817
SmiMI CAYNNNNRTG 1 cut(s) 979
SmlI CTYRAG 2 cut(s) 446, 817
SmoI CTYRAG 2 cut(s) 446, 817
Sse9I AATT 4 cut(s) 624, 963, 1038, 1047
SseBI AGGCCT 1 cut(s) 452
SspDI GGCGCC 1 cut(s) 396
SspMI CTAG 2 cut(s) 479, 1124
SstI GAGCTC 1 cut(s) 429
StuI AGGCCT 1 cut(s) 452
StyD4I CCNGG 3 cut(s) 8, 106, 464
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 2 cut(s) 299, 1230
TaiI ACGT 7 cut(s) 206, 395, 526, 531, 636, 1190, 1211
TaqI TCGA 3 cut(s) 174, 818, 906
TasI AATT 4 cut(s) 624, 963, 1038, 1047
TatI WGTACW 1 cut(s) 579
TauI GCSGC 2 cut(s) 171, 213
TfiI GAWTC 2 cut(s) 676, 745
Tru1I TTAA 2 cut(s) 869, 1109
Tru9I TTAA 2 cut(s) 869, 1109
TscAI CASTG 3 cut(s) 981, 1185, 1233
TseFI GTSAC 3 cut(s) 188, 1030, 1176
TseI GCWGC 2 cut(s) 754, 772
Tsp45I GTSAC 3 cut(s) 188, 1030, 1176
TspDTI ATGAA 5 cut(s) 46, 130, 334, 924, 1115
TspGWI ACGGA 4 cut(s) 19, 176, 426, 805
TspRI CASTG 3 cut(s) 981, 1185, 1233
VpaK11BI GGWCC 2 cut(s) 110, 325
XceI RCATGY 2 cut(s) 200, 586
XhoI CTCGAG 1 cut(s) 817
XmiI GTMKAC 1 cut(s) 957
XmnI GAANNNNTTC 1 cut(s) 1131
XspI CTAG 2 cut(s) 479, 1124
ZraI GACGTC 1 cut(s) 1188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.