Rh5BG246400

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0017815
Physical Location & Seq
Reverse (-)
30040509 .. 30049296
8788 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG246400.1

Sequence Viewer

Length: 1566 bp
ATGGGAGCCTGGCTCCTTATACCCTTCTCCGTCTTCCTCACTTTCACCCTAAAGTTCATCCTCTTCCCCTACAAACCCTCCCACAAACTCAACGCCCCACTCCCTCCGGGTCCCACCACCATACCCGTCGTCGGCACCTTCATATGGCTCTTCAAACTCGCCTCCCGCGGCATCGAGCCAGTCCTCCGTGACCTACATGCCACGTACGGCGGCATCGTCTCTGTCCCCGTCCCCTTCTCTTACCCCGTCATCATGATCGCCGACCGCTCCCTCGCCCACCAAGCCCTTGTCAACAACGGTGCCGTTTTTGCCGACCGCCCTGAGGTCCAACCATCCATCAAGTTCATCACCAGCAACCAACGCATCATCGCCACCGCTGGCTACGGCTCTACGTGGCGCCTCCTGCGCCGGAACCTCGTCTCTGAGCTCATGGTTCCGTCTCGGCTCAAGGCCTTGGCCGGTTCCCGGAAGTGGGCCCTAGACAGCCTGGCCAGCCGTCTTACCACTTCGTCGTCATCGGGACGTAACATGGTGGGAGTTTTGGATCATTTCCGGATGTCCATGACGGGTTTGTTTCTGTACATGTGTTTTGGTGAGAAGATGGAGGAGGAGAAGGTGAGAGAAATTGTGGACGCTCAGCGTCGGCTTCTGTTGGGTTTGGTCGGAGAGTTTCAGATTCTCAACTTTGGCCCTGCATGGTTTACCAAGATTTTGTTTTATAAGCGGTGGAGTTGCTTCTTCCAGGTTCTCCAGCAGCAAATAAACCTCCTGCTGCCTCTGATACAAGCACGGAAGAAGATAAAGGAAAGAAAATGCTCGAGTAACCGAAAAGAAGATAACAACAACAATCAAGCATTTGTTGATACCTTAATGGATTTAGAACTCCCTGACGAGAAACGAACATTCGATGAAGTAGAAGTGGTGAACCAATGCTCGGATTTCCTCAACTCCGGCGTAGACACAATTGCCATTACACTGCAATGGATGATGGCAAACATAGTGAAACACCCACACATTCAAGACAAACTTGTCACAGAAATTAGACAAATTGTGGGAGACGGAGAAGTTGAAGAGGTGGAAGAGGAGAACCTGCACAGAATGAAGTATTTAAAAGCGGTTGTGCTAGAAGGTCTGCTTCTTCACCCGCCGTCGCACTTTTTGCTTCCGCACTCTGTCACTGAGGACGTCATTTTGGACGGGCGTTACGTTATTGTGCCGAAGGACTGCACTGTGAACTTTTCTGTGGCGGAGATGGGGCGGGATCCGAAGGTGTGGGCGGAGCCGATGGAGTTCAAGCCTGAGAGTTTTCTGAGTGATGAGGAGAACTTGTGTGATTTAACTGGTAGTAAGGAGATCAAGATGATGCCGTTTGGGGTAGGGAGGAGGATGTGTCCTGGTTCGAATTTGGCAATGCTGCTTTTGGAGTACTTTGTGGCGAATTTGGTTTGGAAATTCGAGTGGAGAGCTGTGGAGGGAGTTGGGGTTGATTTGAGTGAGAAGGAGGAGACGAGTTGGGGGATGAATAATCCATTGCAGGCACATATCACTCCAAGGTTGAAGACATGA

Protein Analysis

521

Amino Acids

59.22

Weight (kDa)

6.85

Isoelectric Point (pI)

42.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 35 - 504 1.6e-79 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017815)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0035991
rosa_laevigata RLG00000033658
rosa_multiflora Rmu_co8295021.1_g000001 Rmu_sc0000084.1_g000043 Rmu_sc0001493.1_g000014
rosa_roxburghii Rroxscaffold_1G00044750
rosa_rugosa Rorug05G0152000
rosa_samantha Rh5AG246000 Rh5BG246400 Rh5CG277200 Rh5DG254900
rosa_wichuraiana Rw5G022600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 720
AasI GACNNNNNNGTC 1 cut(s) 1028
AatII GACGTC 1 cut(s) 1188
Acc36I ACCTGC 1 cut(s) 1098
AccB1I GGYRCC 3 cut(s) 134, 299, 396
AccBSI CCGCTC 1 cut(s) 267
AccI GTMKAC 1 cut(s) 957
AccII CGCG 1 cut(s) 168
AccIII TCCGGA 1 cut(s) 552
AclWI GGATC 3 cut(s) 552, 1256, 1269
AcoI YGGCCR 2 cut(s) 456, 489
AcsI RAATTY 3 cut(s) 1402, 1438, 1451
AcyI GRCGYC 2 cut(s) 397, 1185
AfaI GTAC 3 cut(s) 206, 581, 1427
AfiI CCNNNNNNNGG 7 cut(s) 131, 144, 322, 465, 471, 472, 934
AflIII ACRYGT 1 cut(s) 582
AgsI TTSAA 5 cut(s) 154, 1019, 1070, 1294, 1558
AjnI CCWGG 4 cut(s) 8, 486, 741, 1393
AluBI AGCT 2 cut(s) 427, 1466
AluI AGCT 2 cut(s) 427, 1466
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 5 cut(s) 223, 424, 444, 1050, 1499
AlwI GGATC 3 cut(s) 552, 1256, 1269
Ama87I CYCGRG 1 cut(s) 817
Aor13HI TCCGGA 1 cut(s) 552
AoxI GGCC 5 cut(s) 450, 456, 474, 489, 688
ApaI GGGCCC 1 cut(s) 478
ApeKI GCWGC 3 cut(s) 754, 772, 1414
ApoI RAATTY 3 cut(s) 1402, 1438, 1451
AspLEI GCGC 2 cut(s) 399, 408
AspS9I GGNCC 5 cut(s) 110, 325, 474, 475, 689
AsuC2I CCSGG 2 cut(s) 108, 466
AsuHPI GGTGA 6 cut(s) 37, 340, 605, 628, 934, 1133
AsuII TTCGAA 1 cut(s) 1400
AvaI CYCGRG 1 cut(s) 817
AvaII GGWCC 2 cut(s) 110, 325
AxyI CCTNAGG 1 cut(s) 321
BaeGI GKGCMC 1 cut(s) 478
BalI TGGCCA 1 cut(s) 491
BamHI GGATCC 1 cut(s) 1261
BanI GGYRCC 3 cut(s) 134, 299, 396
BanII GRGCYC 2 cut(s) 429, 478
BbsI GAAGAC 1 cut(s) 25
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 3 cut(s) 759, 766, 1401
BccI CCATC 6 cut(s) 340, 344, 595, 982, 1246, 1279
BceAI ACGGC 6 cut(s) 223, 287, 400, 480, 1132, 1351
BciT130I CCWGG 4 cut(s) 10, 488, 743, 1395
BcnI CCSGG 2 cut(s) 108, 466
BcoDI GTCTC 5 cut(s) 223, 424, 444, 1050, 1499
BfaI CTAG 2 cut(s) 479, 1124
BfoI RGCGCY 1 cut(s) 400
BfuAI ACCTGC 1 cut(s) 1098
BisI GCNGC 5 cut(s) 169, 211, 755, 773, 1415
BlpI GCTNAGC 1 cut(s) 636
BlsI GCNGC 5 cut(s) 170, 212, 756, 774, 1416
BmcAI AGTACT 1 cut(s) 1427
Bme1390I CCNGG 6 cut(s) 10, 108, 466, 488, 743, 1395
Bme18I GGWCC 2 cut(s) 110, 325
BmeT110I CYCGRG 1 cut(s) 817
BmgT120I GGNCC 5 cut(s) 110, 325, 474, 475, 689
BmrFI CCNGG 6 cut(s) 10, 108, 466, 488, 743, 1395
BmsI GCATC 4 cut(s) 180, 222, 372, 1353
BpiI GAAGAC 1 cut(s) 25
BplI GAGNNNNNCTC 1 cut(s) 29
BpmI CTGGAG 1 cut(s) 734
Bpu1102I GCTNAGC 1 cut(s) 636
Bpu14I TTCGAA 1 cut(s) 1400
BpuEI CTTGAG 1 cut(s) 431
BpuMI CCSGG 2 cut(s) 108, 466
BsaAI YACGTR 2 cut(s) 204, 393
BsaHI GRCGYC 2 cut(s) 397, 1185
BsaJI CCNNGG 3 cut(s) 166, 453, 1550
BsaWI WCCGGW 1 cut(s) 552
BsaXI ACNNNNNCTCC 8 cut(s) 62, 92, 1313, 1343, 1375, 1405, 1467, 1497
Bsc4I CCNNNNNNNGG 7 cut(s) 131, 144, 322, 465, 471, 472, 934
Bse118I RCCGGY 1 cut(s) 458
Bse1I ACTGG 2 cut(s) 179, 1345
Bse21I CCTNAGG 1 cut(s) 321
Bse3DI GCAATG 3 cut(s) 986, 1416, 1529
BseAI TCCGGA 1 cut(s) 552
BseBI CCWGG 4 cut(s) 10, 488, 743, 1395
BseDI CCNNGG 3 cut(s) 166, 453, 1550
BseGI GGATG 6 cut(s) 57, 332, 561, 990, 1392, 1524
BseLI CCNNNNNNNGG 7 cut(s) 131, 144, 322, 465, 471, 472, 934
BseMI GCAATG 3 cut(s) 986, 1416, 1529
BseMII CTCAG 6 cut(s) 312, 414, 650, 1170, 1290, 1301
BseNI ACTGG 2 cut(s) 179, 1345
BseRI GAGGAG 6 cut(s) 620, 623, 1097, 1334, 1396, 1517
BseSI GKGCMC 1 cut(s) 478
BseXI GCAGC 3 cut(s) 759, 766, 1401
BsgI GTGCAG 2 cut(s) 1076, 1210
Bsh1236I CGCG 1 cut(s) 168
Bsh1285I CGRYCG 2 cut(s) 265, 316
BshFI GGCC 5 cut(s) 452, 458, 476, 491, 690
BshNI GGYRCC 3 cut(s) 134, 299, 396
BsiEI CGRYCG 2 cut(s) 265, 316
BsiHKAI GWGCWC 1 cut(s) 429
BsiHKCI CYCGRG 1 cut(s) 817
BsiSI CCGG 6 cut(s) 107, 409, 459, 466, 553, 951
BsiWI CGTACG 1 cut(s) 204
BslFI GGGAC 4 cut(s) 96, 209, 215, 534
BslI CCNNNNNNNGG 7 cut(s) 131, 144, 322, 465, 471, 472, 934
BsmAI GTCTC 5 cut(s) 223, 424, 444, 1050, 1499
BsmBI CGTCTC 5 cut(s) 223, 424, 444, 1050, 1499
BsmFI GGGAC 4 cut(s) 96, 209, 215, 534
BsnI GGCC 5 cut(s) 452, 458, 476, 491, 690
BsoBI CYCGRG 1 cut(s) 817
Bsp119I TTCGAA 1 cut(s) 1400
Bsp120I GGGCCC 1 cut(s) 474
Bsp1286I GDGCHC 2 cut(s) 429, 478
Bsp13I TCCGGA 1 cut(s) 552
Bsp1407I TGTACA 1 cut(s) 579
Bsp143I GATC 4 cut(s) 255, 544, 1261, 1353
Bsp1720I GCTNAGC 1 cut(s) 636
BspANI GGCC 5 cut(s) 452, 458, 476, 491, 690
BspCNI CTCAG 6 cut(s) 313, 415, 649, 1171, 1291, 1302
BspEI TCCGGA 1 cut(s) 552
BspFNI CGCG 1 cut(s) 168
BspHI TCATGA 1 cut(s) 252
BspMI ACCTGC 1 cut(s) 1098
BspPI GGATC 3 cut(s) 552, 1256, 1269
BspQI GCTCTTC 1 cut(s) 155
BspT104I TTCGAA 1 cut(s) 1400
BspT107I GGYRCC 3 cut(s) 134, 299, 396
BsrBI CCGCTC 1 cut(s) 267
BsrDI GCAATG 3 cut(s) 986, 1416, 1529
BsrFI RCCGGY 1 cut(s) 458
BsrGI TGTACA 1 cut(s) 579
BsrI ACTGG 2 cut(s) 179, 1345
BssAI RCCGGY 1 cut(s) 458
BssECI CCNNGG 3 cut(s) 166, 453, 1550
BssMI GATC 4 cut(s) 255, 544, 1261, 1353
BssNI GRCGYC 2 cut(s) 397, 1185
BssT1I CCWWGG 2 cut(s) 453, 1550
Bst2UI CCWGG 4 cut(s) 10, 488, 743, 1395
Bst4CI ACNGT 2 cut(s) 299, 1231
Bst6I CTCTTC 4 cut(s) 68, 155, 1065, 1074
BstACI GRCGYC 2 cut(s) 397, 1185
BstAPI GCANNNNNTGC 1 cut(s) 1159
BstAUI TGTACA 1 cut(s) 579
BstBAI YACGTR 2 cut(s) 204, 393
BstBI TTCGAA 1 cut(s) 1400
BstC8I GCNNGC 3 cut(s) 379, 493, 1536
BstDEI CTNAG 6 cut(s) 321, 423, 636, 1179, 1299, 1310
BstDSI CCRYGG 1 cut(s) 166
BstF5I GGATG 6 cut(s) 57, 332, 561, 990, 1392, 1524
BstFNI CGCG 1 cut(s) 168
BstH2I RGCGCY 1 cut(s) 400
BstHHI GCGC 2 cut(s) 399, 408
BstKTI GATC 4 cut(s) 258, 547, 1264, 1356
BstMAI GTCTC 5 cut(s) 223, 424, 444, 1050, 1499
BstMBI GATC 4 cut(s) 255, 544, 1261, 1353
BstMCI CGRYCG 2 cut(s) 265, 316
BstMWI GCNNNNNNNGC 7 cut(s) 281, 308, 360, 403, 405, 492, 1159
BstNI CCWGG 4 cut(s) 10, 488, 743, 1395
BstNSI RCATGY 2 cut(s) 200, 586
BstSCI CCNGG 6 cut(s) 8, 106, 464, 486, 741, 1393
BstSLI GKGCMC 1 cut(s) 478
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 3 cut(s) 759, 766, 1401
BstV2I GAAGAC 1 cut(s) 25
BstX2I RGATCY 1 cut(s) 1261
BstYI RGATCY 1 cut(s) 1261
Bsu36I CCTNAGG 1 cut(s) 321
BsuRI GGCC 5 cut(s) 452, 458, 476, 491, 690
BtgI CCRYGG 1 cut(s) 166
BtgZI GCGATG 1 cut(s) 352
BtsCI GGATG 6 cut(s) 57, 332, 561, 990, 1392, 1524
BtsI GCAGTG 1 cut(s) 974
BtsIMutI CAGTG 3 cut(s) 974, 1176, 1227
BveI ACCTGC 1 cut(s) 1098
Cac8I GCNNGC 3 cut(s) 379, 493, 1536
CciI TCATGA 1 cut(s) 252
CfoI GCGC 2 cut(s) 399, 408
Cfr10I RCCGGY 1 cut(s) 458
Cfr13I GGNCC 5 cut(s) 110, 325, 474, 475, 689
Cfr42I CCGCGG 1 cut(s) 169
CseI GACGC 2 cut(s) 629, 641
Csp6I GTAC 3 cut(s) 205, 580, 1426
CviAII CATG 8 cut(s) 197, 253, 430, 529, 562, 583, 696, 1563
CviQI GTAC 3 cut(s) 205, 580, 1426
DdeI CTNAG 6 cut(s) 321, 423, 636, 1179, 1299, 1310
DinI GGCGCC 1 cut(s) 398
DpnI GATC 4 cut(s) 257, 546, 1263, 1355
DpnII GATC 4 cut(s) 255, 544, 1261, 1353
DraI TTTAAA 1 cut(s) 1110
DrdI GACNNNNNNGTC 1 cut(s) 1028
DseDI GACNNNNNNGTC 1 cut(s) 1028
EaeI YGGCCR 2 cut(s) 456, 489
Eam1104I CTCTTC 4 cut(s) 68, 155, 1065, 1074
EarI CTCTTC 4 cut(s) 68, 155, 1065, 1074
EciI GGCGGA 2 cut(s) 1262, 1292
Ecl136II GAGCTC 1 cut(s) 427
Eco130I CCWWGG 2 cut(s) 453, 1550
Eco147I AGGCCT 1 cut(s) 452
Eco24I GRGCYC 2 cut(s) 429, 478
Eco47I GGWCC 2 cut(s) 110, 325
Eco53kI GAGCTC 1 cut(s) 427
Eco81I CCTNAGG 1 cut(s) 321
Eco88I CYCGRG 1 cut(s) 817
EcoICRI GAGCTC 1 cut(s) 427
EcoO109I RGGNCCY 2 cut(s) 110, 475
EcoRII CCWGG 4 cut(s) 8, 486, 741, 1393
EcoT14I CCWWGG 2 cut(s) 453, 1550
EcoT38I GRGCYC 2 cut(s) 429, 478
EgeI GGCGCC 1 cut(s) 398
EheI GGCGCC 1 cut(s) 398
ErhI CCWWGG 2 cut(s) 453, 1550
Esp3I CGTCTC 5 cut(s) 223, 424, 444, 1050, 1499
FaeI CATG 8 cut(s) 200, 256, 433, 532, 565, 586, 699, 1566
FalI AAGNNNNNCTT 4 cut(s) 1011, 1043, 1119, 1151
FaqI GGGAC 4 cut(s) 96, 209, 215, 534
FatI CATG 8 cut(s) 196, 252, 429, 528, 561, 582, 695, 1562
FauI CCCGC 3 cut(s) 173, 1152, 1251
FauNDI CATATG 1 cut(s) 143
FblI GTMKAC 1 cut(s) 957
Fnu4HI GCNGC 5 cut(s) 169, 211, 755, 773, 1415
FokI GGATG 6 cut(s) 44, 319, 568, 997, 1399, 1531
FriOI GRGCYC 2 cut(s) 429, 478
Fsp4HI GCNGC 5 cut(s) 169, 211, 755, 773, 1415
FspBI CTAG 2 cut(s) 479, 1124
GlaI GCGC 2 cut(s) 398, 407
GluI GCNGC 5 cut(s) 169, 211, 755, 773, 1415
GsuI CTGGAG 1 cut(s) 734
HaeII RGCGCY 1 cut(s) 400
HaeIII GGCC 5 cut(s) 452, 458, 476, 491, 690
HapII CCGG 6 cut(s) 107, 409, 459, 466, 553, 951
HgaI GACGC 2 cut(s) 629, 641
HhaI GCGC 2 cut(s) 399, 408
Hin1I GRCGYC 2 cut(s) 397, 1185
Hin1II CATG 8 cut(s) 200, 256, 433, 532, 565, 586, 699, 1566
Hin6I GCGC 2 cut(s) 397, 406
HinP1I GCGC 2 cut(s) 397, 406
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 1 cut(s) 676
HpaII CCGG 6 cut(s) 107, 409, 459, 466, 553, 951
HphI GGTGA 6 cut(s) 37, 340, 605, 628, 934, 1133
Hpy166II GTNNAC 6 cut(s) 292, 631, 702, 925, 958, 1234
Hpy188I TCNGA 7 cut(s) 424, 665, 675, 780, 937, 1266, 1311
Hpy188III TCNNGA 5 cut(s) 253, 519, 553, 1019, 1357
Hpy8I GTNNAC 6 cut(s) 292, 631, 702, 925, 958, 1234
Hpy99I CGWCG 5 cut(s) 131, 134, 514, 645, 1153
HpyAV CCTTC 8 cut(s) 34, 148, 244, 607, 1121, 1213, 1261, 1492
HpyCH4III ACNGT 2 cut(s) 299, 1231
HpyCH4IV ACGT 5 cut(s) 203, 392, 523, 1185, 1206
HpyCH4V TGCA 5 cut(s) 695, 979, 1093, 1227, 1534
HpyF10VI GCNNNNNNNGC 7 cut(s) 281, 308, 360, 403, 405, 492, 1159
HpyF3I CTNAG 6 cut(s) 321, 423, 636, 1179, 1299, 1310
HpySE526I ACGT 5 cut(s) 203, 392, 523, 1185, 1206
Hsp92I GRCGYC 2 cut(s) 397, 1185
Hsp92II CATG 8 cut(s) 200, 256, 433, 532, 565, 586, 699, 1566
HspAI GCGC 2 cut(s) 397, 406
KasI GGCGCC 1 cut(s) 396
KflI GGGWCCC 1 cut(s) 110
Kpn2I TCCGGA 1 cut(s) 552
KspI CCGCGG 1 cut(s) 169
Kzo9I GATC 4 cut(s) 255, 544, 1261, 1353
LguI GCTCTTC 1 cut(s) 155
LmnI GCTCC 4 cut(s) 5, 18, 272, 1279
Lsp1109I GCAGC 3 cut(s) 759, 766, 1401
LweI GCATC 4 cut(s) 180, 222, 372, 1353
MaeI CTAG 2 cut(s) 479, 1124
MaeII ACGT 5 cut(s) 203, 392, 523, 1185, 1206
MaeIII GTNAC 6 cut(s) 188, 524, 821, 1030, 1174, 1202
MalI GATC 4 cut(s) 257, 546, 1263, 1355
MbiI CCGCTC 1 cut(s) 267
MboI GATC 4 cut(s) 255, 544, 1261, 1353
MfeI CAATTG 1 cut(s) 963
MflI RGATCY 1 cut(s) 1261
MhlI GDGCHC 2 cut(s) 429, 478
MlsI TGGCCA 1 cut(s) 491
MluCI AATT 7 cut(s) 624, 963, 1038, 1047, 1402, 1438, 1451
MluNI TGGCCA 1 cut(s) 491
Mly113I GGCGCC 1 cut(s) 397
MmeI TCCRAC 2 cut(s) 352, 643
Mox20I TGGCCA 1 cut(s) 491
MroI TCCGGA 1 cut(s) 552
MscI TGGCCA 1 cut(s) 491
MseI TTAA 3 cut(s) 869, 1109, 1337
MslI CAYNNNNRTG 1 cut(s) 979
Msp20I TGGCCA 1 cut(s) 491
MspA1I CMGCKG 2 cut(s) 168, 377
MspI CCGG 6 cut(s) 107, 409, 459, 466, 553, 951
MspR9I CCNGG 6 cut(s) 10, 108, 466, 488, 743, 1395
MunI CAATTG 1 cut(s) 963
MvaI CCWGG 4 cut(s) 10, 488, 743, 1395
MvnI CGCG 1 cut(s) 168
MwoI GCNNNNNNNGC 7 cut(s) 281, 308, 360, 403, 405, 492, 1159
NarI GGCGCC 1 cut(s) 397
NciI CCSGG 2 cut(s) 108, 466
NdeI CATATG 1 cut(s) 143
NdeII GATC 4 cut(s) 255, 544, 1261, 1353
NlaIII CATG 8 cut(s) 200, 256, 433, 532, 565, 586, 699, 1566
NmeAIII GCCGAG 1 cut(s) 421
NmuCI GTSAC 3 cut(s) 188, 1030, 1174
NspI RCATGY 2 cut(s) 200, 586
NspV TTCGAA 1 cut(s) 1400
PaeR7I CTCGAG 1 cut(s) 817
PagI TCATGA 1 cut(s) 252
PceI AGGCCT 1 cut(s) 452
PciI ACATGT 1 cut(s) 582
PciSI GCTCTTC 1 cut(s) 155
PcsI WCGNNNNNNNCGW 2 cut(s) 213, 1203
PfeI GAWTC 1 cut(s) 676
Pfl23II CGTACG 1 cut(s) 204
PfoI TCCNGGA 1 cut(s) 464
PkrI GCNGC 5 cut(s) 170, 212, 756, 774, 1416
PluTI GGCGCC 1 cut(s) 400
Ppu21I YACGTR 2 cut(s) 204, 393
PpuMI RGGWCCY 1 cut(s) 110
PscI ACATGT 1 cut(s) 582
PsiI TTATAA 1 cut(s) 720
Psp124BI GAGCTC 1 cut(s) 429
Psp5II RGGWCCY 1 cut(s) 110
Psp6I CCWGG 4 cut(s) 8, 486, 741, 1393
PspGI CCWGG 4 cut(s) 8, 486, 741, 1393
PspLI CGTACG 1 cut(s) 204
PspOMI GGGCCC 1 cut(s) 474
PspPI GGNCC 5 cut(s) 110, 325, 474, 475, 689
PspPPI RGGWCCY 1 cut(s) 110
PspXI VCTCGAGB 1 cut(s) 817
PsuI RGATCY 1 cut(s) 1261
RsaI GTAC 3 cut(s) 206, 581, 1427
RsaNI GTAC 3 cut(s) 205, 580, 1426
RseI CAYNNNNRTG 1 cut(s) 979
SacI GAGCTC 1 cut(s) 429
SacII CCGCGG 1 cut(s) 169
SapI GCTCTTC 1 cut(s) 155
SaqAI TTAA 3 cut(s) 869, 1109, 1337
SatI GCNGC 5 cut(s) 169, 211, 755, 773, 1415
Sau3AI GATC 4 cut(s) 255, 544, 1261, 1353
Sau96I GGNCC 5 cut(s) 110, 325, 474, 475, 689
ScaI AGTACT 1 cut(s) 1427
ScrFI CCNGG 6 cut(s) 10, 108, 466, 488, 743, 1395
SduI GDGCHC 2 cut(s) 429, 478
SfaNI GCATC 4 cut(s) 180, 222, 372, 1353
SfoI GGCGCC 1 cut(s) 398
Sfr274I CTCGAG 1 cut(s) 817
Sfr303I CCGCGG 1 cut(s) 169
SfuI TTCGAA 1 cut(s) 1400
SgrBI CCGCGG 1 cut(s) 169
SinI GGWCC 2 cut(s) 110, 325
SlaI CTCGAG 1 cut(s) 817
SmiMI CAYNNNNRTG 1 cut(s) 979
SmlI CTYRAG 2 cut(s) 446, 817
SmoI CTYRAG 2 cut(s) 446, 817
Sse9I AATT 7 cut(s) 624, 963, 1038, 1047, 1402, 1438, 1451
SseBI AGGCCT 1 cut(s) 452
SspDI GGCGCC 1 cut(s) 396
SspMI CTAG 2 cut(s) 479, 1124
SstI GAGCTC 1 cut(s) 429
StuI AGGCCT 1 cut(s) 452
StyD4I CCNGG 6 cut(s) 8, 106, 464, 486, 741, 1393
StyI CCWWGG 2 cut(s) 453, 1550
TaaI ACNGT 2 cut(s) 299, 1231
TaiI ACGT 5 cut(s) 206, 395, 526, 1188, 1209
TaqI TCGA 5 cut(s) 174, 818, 906, 1400, 1455
TasI AATT 7 cut(s) 624, 963, 1038, 1047, 1402, 1438, 1451
TatI WGTACW 2 cut(s) 579, 1425
TauI GCSGC 2 cut(s) 171, 213
TfiI GAWTC 1 cut(s) 676
Tru1I TTAA 3 cut(s) 869, 1109, 1337
Tru9I TTAA 3 cut(s) 869, 1109, 1337
TscAI CASTG 3 cut(s) 981, 1183, 1234
TseFI GTSAC 3 cut(s) 188, 1030, 1174
TseI GCWGC 3 cut(s) 754, 772, 1414
Tsp45I GTSAC 3 cut(s) 188, 1030, 1174
TspDTI ATGAA 6 cut(s) 46, 130, 334, 924, 1115, 1535
TspGWI ACGGA 5 cut(s) 19, 176, 426, 805, 1074
TspRI CASTG 3 cut(s) 981, 1183, 1234
VpaK11BI GGWCC 2 cut(s) 110, 325
XapI RAATTY 3 cut(s) 1402, 1438, 1451
XceI RCATGY 2 cut(s) 200, 586
XhoI CTCGAG 1 cut(s) 817
XmiI GTMKAC 1 cut(s) 957
XspI CTAG 2 cut(s) 479, 1124
ZraI GACGTC 1 cut(s) 1186
ZrmI AGTACT 1 cut(s) 1427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.