RLG00000033745

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0013641
Physical Location & Seq
Reverse (-)
31650077 .. 31651804
1728 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033745

Sequence Viewer

Length: 1728 bp
ATGGACTCGCACCAGCATTTTGGTTTCAGTGAAACTGGTGCAGGCTATTTTGCAGGTTCTCCTCGCACTGTTCCATCTATACCTAACAGGTTGCTTGGGTCTTTGAAATTAGATATTGGTAACTCACCAAATTCACCATTTTCAACTCACTTTGATTCTGATACTGCTACTACGCTGAGTGACTGCCAGGAGCAACTCAGCTCCACAGAAAATCTCTCAGGCGTCAGCCATTCCTCTAACTCTCCATTTGAAACCAACAGTTATATTTCTCAGTTAAGCTTAAGCCCTTCTGTGGATTGTTGTCGAGACAGTCTGCAGCTCTCTTCTGGTAGGGCATCTTTGTTACAGGATGCAAATTGTAGCCCCAATATAAAATACGCTTTGCAGGAATTGGAGAGTGCTTTAATGGGGCCTGATGAAGAAGAGGTCACCGCAACAAACATTTTTGGGGAAACTAGCAGGCGGAAGGGACAGAGATCTCTGTCATGGAACCAGGAGCGCGCGGGTGCACATGTGATGCAGCGCCAGACTTCCTTTGTTTCAGGGCATAGACAGTCAGAGAAACGTCACAAGGTGCTTGATGAAGCATCTCCACAGAAACCGGCTGATAACCTGCGACAATTGCTGATTGCATGTGCTGAAGCACTTTCTGACAACAATATGGATGGTTTTGAGGAGTTGATTGAAAAGGCTAGAGGTGCTGTGTCTATCAGTGGAGAACCAATTCAGCGTCTTGGAGCTTACATGGTAGAAGGGCTGGTTGCAAGGAAGGAGGCTTCGGGCTCCAATATCTACCGTGCCCTCAGGTGCAGGGAGCCTGAAAGCGATGACTTGCTCTCATACATGCACATTCTGTATGAGATCTGCCCCTATTTAAAGTTCGGTTACATGGCAGCCAATGGGGCCATTGCTGACGCCTGCAGAAATGAGGACCGTATCCACATTATAGATTTCCAGATTGCTCAGGGTACTCAATGGGTGACTCTCCTTCAAGCTCTTGCAGCAAGACCCAGTGGGGCTCCCCATGTACGAATTACAGGTATCGATGACCCTGTTTCTAAATATGCTCGTGGTGATGGCTTGGAGGCAGTAGGAAGACGGTTGAAAGCAATCTCTGAGAAATTTAACATCCCAGTTGAATTTCATGGTGTTCCAGTTTTTGCACCTGATGTCACACGGGACATGCTTGATGTCAGGCCTGGTGAGGCTCTAGCAGTGAACTTTCCCCTGCAGCTTCACCACACACCAGATGAAAGCGTTGATGTGAACAATCCAAGGGATGGTCTTCTGAGGATAGTCAAGTCACTATCTCCAAAGGTGACTACTTTGGTTGAGCAAGAATCAAACACGAATACAACCCCTTTCTTGAATCGGTTTATAGAGACTCTAGACTACTATTTGGCAATGTTTGAGTCTATTGACGTGACCCTGCCGAGGAACAGCAAGGAGAGGATCAACGTGGAGCAGCATTGTCTGGCTCGAGATATTGTAAATGTGATTGCCTGCGAAGGAAAGGAAAGGGTGGAACGCCACGAGCTCTTTGGAAAGTGGAAGTCCAGGTTGACAATGGCAGGATTTCGGCAATACCCATTAAGCTCATATGTCAACTCTGTCATAAAGAGTCTACTGAGGTGTTACTCAGAGCATTATAAGCTGGAGGAGAAGGATGGTGCTATGCTGTTGGGATGGAAGGAGAGGAACTTGGTATCAGCTTCTGCGTGGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

576

Amino Acids

63.98

Weight (kDa)

5.77

Isoelectric Point (pI)

51.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 205 - 574 1.5e-135 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1650
Acc36I ACCTGC 2 cut(s) 44, 621
AccB7I CCANNNNNTGG 1 cut(s) 1280
AccI GTMKAC 1 cut(s) 1624
AccII CGCG 2 cut(s) 501, 503
AciI CCGC 3 cut(s) 432, 463, 503
AclWI GGATC 1 cut(s) 1460
AcsI RAATTY 3 cut(s) 130, 1121, 1139
AcuI CTGAAG 1 cut(s) 660
AcyI GRCGYC 2 cut(s) 222, 915
AdeI CACNNNGTG 1 cut(s) 574
AfaI GTAC 2 cut(s) 970, 1029
AfiI CCNNNNNNNGG 3 cut(s) 292, 1280, 1434
AflII CTTAAG 1 cut(s) 280
AflIII ACRYGT 1 cut(s) 511
AgsI TTSAA 8 cut(s) 106, 144, 251, 686, 992, 1105, 1139, 1369
AjiI CACGTC 1 cut(s) 1423
AjnI CCWGG 4 cut(s) 186, 492, 1198, 1556
Alw21I GWGCWC 2 cut(s) 511, 1539
Alw26I GTCTC 2 cut(s) 300, 1376
Alw44I GTGCAC 1 cut(s) 507
AlwI GGATC 1 cut(s) 1460
AlwNI CAGNNNCTG 1 cut(s) 1715
Ama87I CYCGRG 1 cut(s) 1479
AoxI GGCC 3 cut(s) 410, 903, 1196
ApaLI GTGCAC 1 cut(s) 507
ApeKI GCWGC 6 cut(s) 316, 520, 893, 1001, 1231, 1465
ApoI RAATTY 3 cut(s) 130, 1121, 1139
Asp700I GAANNNNTTC 1 cut(s) 723
AspLEI GCGC 3 cut(s) 501, 503, 525
AspS9I GGNCC 3 cut(s) 410, 903, 931
AsuHPI GGTGA 8 cut(s) 117, 126, 421, 991, 1085, 1214, 1229, 1330
AvaI CYCGRG 1 cut(s) 1479
AvaII GGWCC 1 cut(s) 931
AxyI CCTNAGG 1 cut(s) 803
BaeGI GKGCMC 2 cut(s) 511, 802
BanII GRGCYC 3 cut(s) 785, 1021, 1539
BauI CACGAG 2 cut(s) 1068, 1532
BbsI GAAGAC 2 cut(s) 1102, 1277
Bbv12I GWGCWC 2 cut(s) 511, 1539
BbvI GCAGC 6 cut(s) 328, 532, 905, 1013, 1243, 1477
BccI CCATC 6 cut(s) 82, 659, 1070, 1274, 1661, 1680
BciT130I CCWGG 4 cut(s) 188, 494, 1200, 1558
BciVI GTATCC 1 cut(s) 947
BcoDI GTCTC 2 cut(s) 300, 1376
BfaI CTAG 4 cut(s) 456, 693, 1211, 1388
BfmI CTRYAG 3 cut(s) 314, 919, 1229
BfoI RGCGCY 1 cut(s) 526
BfrI CTTAAG 1 cut(s) 280
BfuAI ACCTGC 2 cut(s) 44, 621
BfuI GTATCC 1 cut(s) 947
BglI GCCNNNNNGGC 1 cut(s) 902
BglII AGATCT 2 cut(s) 476, 861
BisI GCNGC 6 cut(s) 317, 521, 894, 1002, 1232, 1466
BlsI GCNGC 6 cut(s) 318, 522, 895, 1003, 1233, 1467
Bme1390I CCNGG 4 cut(s) 188, 494, 1200, 1558
Bme18I GGWCC 1 cut(s) 931
BmeT110I CYCGRG 1 cut(s) 1479
BmgBI CACGTC 1 cut(s) 1423
BmgT120I GGNCC 3 cut(s) 410, 903, 931
BmiI GGNNCC 6 cut(s) 411, 491, 784, 816, 904, 1020
BmrFI CCNGG 4 cut(s) 188, 494, 1200, 1558
BmrI ACTGGG 2 cut(s) 1005, 1127
BmsI GCATC 4 cut(s) 340, 344, 507, 596
BmuI ACTGGG 2 cut(s) 1005, 1127
BpiI GAAGAC 2 cut(s) 1102, 1277
BpmI CTGGAG 1 cut(s) 1676
Bpu10I CCTNAGC 1 cut(s) 963
Bsa29I ATCGAT 1 cut(s) 1044
BsaHI GRCGYC 2 cut(s) 222, 915
BsaJI CCNNGG 2 cut(s) 1274, 1433
Bsc4I CCNNNNNNNGG 3 cut(s) 292, 1280, 1434
Bse118I RCCGGY 1 cut(s) 601
Bse1I ACTGG 4 cut(s) 40, 1011, 1133, 1154
Bse21I CCTNAGG 1 cut(s) 803
Bse3DI GCAATG 2 cut(s) 906, 1410
BseBI CCWGG 4 cut(s) 188, 494, 1200, 1558
BseCI ATCGAT 1 cut(s) 1044
BseDI CCNNGG 2 cut(s) 1274, 1433
BseGI GGATG 6 cut(s) 355, 670, 1128, 1285, 1672, 1691
BseLI CCNNNNNNNGG 3 cut(s) 292, 1280, 1434
BseMI GCAATG 2 cut(s) 906, 1410
BseNI ACTGG 4 cut(s) 40, 1011, 1133, 1154
BsePI GCGCGC 1 cut(s) 499
BseRI GAGGAG 3 cut(s) 51, 689, 1673
BseSI GKGCMC 2 cut(s) 511, 802
BseXI GCAGC 6 cut(s) 328, 532, 905, 1013, 1243, 1477
BsgI GTGCAG 2 cut(s) 60, 829
Bsh1236I CGCG 2 cut(s) 501, 503
BshFI GGCC 3 cut(s) 412, 905, 1198
BshVI ATCGAT 1 cut(s) 1044
BsiHKAI GWGCWC 2 cut(s) 511, 1539
BsiHKCI CYCGRG 1 cut(s) 1479
BsiSI CCGG 1 cut(s) 602
BslFI GGGAC 2 cut(s) 483, 1193
BslI CCNNNNNNNGG 3 cut(s) 292, 1280, 1434
BsmAI GTCTC 2 cut(s) 300, 1376
BsmFI GGGAC 2 cut(s) 483, 1193
BsnI GGCC 3 cut(s) 412, 905, 1198
BsoBI CYCGRG 1 cut(s) 1479
Bsp1286I GDGCHC 5 cut(s) 511, 785, 802, 1021, 1539
Bsp143I GATC 3 cut(s) 476, 861, 1452
BspACI CCGC 3 cut(s) 432, 463, 503
BspANI GGCC 3 cut(s) 412, 905, 1198
BspDI ATCGAT 1 cut(s) 1044
BspFNI CGCG 2 cut(s) 501, 503
BspLI GGNNCC 6 cut(s) 411, 491, 784, 816, 904, 1020
BspMAI CTGCAG 3 cut(s) 318, 923, 1233
BspMI ACCTGC 2 cut(s) 44, 621
BspPI GGATC 1 cut(s) 1460
BspTI CTTAAG 1 cut(s) 280
BsrDI GCAATG 2 cut(s) 906, 1410
BsrFI RCCGGY 1 cut(s) 601
BsrI ACTGG 4 cut(s) 40, 1011, 1133, 1154
BssAI RCCGGY 1 cut(s) 601
BssECI CCNNGG 2 cut(s) 1274, 1433
BssHII GCGCGC 1 cut(s) 499
BssMI GATC 3 cut(s) 476, 861, 1452
BssNI GRCGYC 2 cut(s) 222, 915
BssSI CACGAG 2 cut(s) 1068, 1532
BssT1I CCWWGG 1 cut(s) 1274
Bst2BI CACGAG 2 cut(s) 1068, 1532
Bst2UI CCWGG 4 cut(s) 188, 494, 1200, 1558
Bst4CI ACNGT 7 cut(s) 70, 260, 311, 555, 797, 935, 1101
Bst6I CTCTTC 2 cut(s) 328, 417
BstACI GRCGYC 2 cut(s) 222, 915
BstAFI CTTAAG 1 cut(s) 280
BstC8I GCNNGC 5 cut(s) 43, 461, 501, 919, 1504
BstEII GGTNACC 1 cut(s) 427
BstF5I GGATG 6 cut(s) 355, 670, 1128, 1285, 1672, 1691
BstFNI CGCG 2 cut(s) 501, 503
BstH2I RGCGCY 1 cut(s) 526
BstHHI GCGC 3 cut(s) 501, 503, 525
BstKTI GATC 3 cut(s) 479, 864, 1455
BstMAI GTCTC 2 cut(s) 300, 1376
BstMBI GATC 3 cut(s) 476, 861, 1452
BstMWI GCNNNNNNNGC 5 cut(s) 622, 698, 902, 1001, 1651
BstNI CCWGG 4 cut(s) 188, 494, 1200, 1558
BstNSI RCATGY 4 cut(s) 515, 636, 847, 1186
BstPI GGTNACC 1 cut(s) 427
BstSCI CCNGG 4 cut(s) 186, 492, 1198, 1556
BstSFI CTRYAG 3 cut(s) 314, 919, 1229
BstSLI GKGCMC 2 cut(s) 511, 802
BstUI CGCG 2 cut(s) 501, 503
BstV1I GCAGC 6 cut(s) 328, 532, 905, 1013, 1243, 1477
BstV2I GAAGAC 2 cut(s) 1102, 1277
BstX2I RGATCY 2 cut(s) 476, 861
BstXI CCANNNNNNTGG 1 cut(s) 20
BstYI RGATCY 2 cut(s) 476, 861
Bsu15I ATCGAT 1 cut(s) 1044
Bsu36I CCTNAGG 1 cut(s) 803
BsuI GTATCC 1 cut(s) 947
BsuRI GGCC 3 cut(s) 412, 905, 1198
BsuTUI ATCGAT 1 cut(s) 1044
BtgZI GCGATG 1 cut(s) 840
BtrI CACGTC 1 cut(s) 1423
BtsCI GGATG 6 cut(s) 355, 670, 1128, 1285, 1672, 1691
BtsI GCAGTG 1 cut(s) 1221
BtsIMutI CAGTG 6 cut(s) 34, 66, 718, 1018, 1221, 1723
BveI ACCTGC 2 cut(s) 44, 621
Cac8I GCNNGC 5 cut(s) 43, 461, 501, 919, 1504
CaiI CAGNNNCTG 1 cut(s) 1715
CfoI GCGC 3 cut(s) 501, 503, 525
Cfr10I RCCGGY 1 cut(s) 601
Cfr13I GGNCC 3 cut(s) 410, 903, 931
ClaI ATCGAT 1 cut(s) 1044
CseI GACGC 3 cut(s) 211, 719, 923
Csp6I GTAC 2 cut(s) 969, 1028
CviAII CATG 9 cut(s) 486, 512, 633, 745, 844, 889, 1025, 1145, 1183
CviQI GTAC 2 cut(s) 969, 1028
DpnI GATC 3 cut(s) 478, 863, 1454
DpnII GATC 3 cut(s) 476, 861, 1452
DraI TTTAAA 1 cut(s) 876
DraIII CACNNNGTG 1 cut(s) 574
Eam1104I CTCTTC 2 cut(s) 328, 417
EarI CTCTTC 2 cut(s) 328, 417
EciI GGCGGA 1 cut(s) 478
Ecl136II GAGCTC 1 cut(s) 1537
Eco130I CCWWGG 1 cut(s) 1274
Eco147I AGGCCT 1 cut(s) 1198
Eco24I GRGCYC 3 cut(s) 785, 1021, 1539
Eco47I GGWCC 1 cut(s) 931
Eco53kI GAGCTC 1 cut(s) 1537
Eco57I CTGAAG 1 cut(s) 660
Eco81I CCTNAGG 1 cut(s) 803
Eco88I CYCGRG 1 cut(s) 1479
Eco91I GGTNACC 1 cut(s) 427
EcoICRI GAGCTC 1 cut(s) 1537
EcoO109I RGGNCCY 1 cut(s) 410
EcoO65I GGTNACC 1 cut(s) 427
EcoRII CCWGG 4 cut(s) 186, 492, 1198, 1556
EcoT14I CCWWGG 1 cut(s) 1274
EcoT38I GRGCYC 3 cut(s) 785, 1021, 1539
ErhI CCWWGG 1 cut(s) 1274
FaeI CATG 9 cut(s) 489, 515, 636, 748, 847, 892, 1028, 1148, 1186
FaqI GGGAC 2 cut(s) 483, 1193
FatI CATG 9 cut(s) 485, 511, 632, 744, 843, 888, 1024, 1144, 1182
FauI CCCGC 1 cut(s) 496
FauNDI CATATG 1 cut(s) 1600
FblI GTMKAC 1 cut(s) 1624
Fnu4HI GCNGC 6 cut(s) 317, 521, 894, 1002, 1232, 1466
FokI GGATG 6 cut(s) 362, 677, 1115, 1292, 1679, 1698
FriOI GRGCYC 3 cut(s) 785, 1021, 1539
Fsp4HI GCNGC 6 cut(s) 317, 521, 894, 1002, 1232, 1466
FspBI CTAG 4 cut(s) 456, 693, 1211, 1388
GlaI GCGC 3 cut(s) 500, 502, 524
GluI GCNGC 6 cut(s) 317, 521, 894, 1002, 1232, 1466
GsuI CTGGAG 1 cut(s) 1676
HaeII RGCGCY 1 cut(s) 526
HaeIII GGCC 3 cut(s) 412, 905, 1198
HapII CCGG 1 cut(s) 602
HgaI GACGC 3 cut(s) 211, 719, 923
HhaI GCGC 3 cut(s) 501, 503, 525
Hin1I GRCGYC 2 cut(s) 222, 915
Hin1II CATG 9 cut(s) 489, 515, 636, 748, 847, 892, 1028, 1148, 1186
Hin6I GCGC 3 cut(s) 499, 501, 523
HinP1I GCGC 3 cut(s) 499, 501, 523
HincII GTYRAC 2 cut(s) 1563, 1606
HindII GTYRAC 2 cut(s) 1563, 1606
HindIII AAGCTT 1 cut(s) 277
HinfI GANTC 8 cut(s) 5, 155, 982, 1340, 1369, 1384, 1412, 1621
HpaII CCGG 1 cut(s) 602
HphI GGTGA 8 cut(s) 117, 126, 421, 991, 1085, 1214, 1229, 1330
Hpy166II GTNNAC 6 cut(s) 509, 1219, 1267, 1563, 1606, 1625
Hpy188I TCNGA 6 cut(s) 160, 559, 652, 1117, 1290, 1642
Hpy188III TCNNGA 5 cut(s) 305, 955, 1366, 1388, 1481
Hpy8I GTNNAC 6 cut(s) 509, 1219, 1267, 1563, 1606, 1625
HpyAV CCTTC 8 cut(s) 297, 460, 746, 763, 998, 1502, 1657, 1684
HpyCH4III ACNGT 7 cut(s) 70, 260, 311, 555, 797, 935, 1101
HpyCH4IV ACGT 3 cut(s) 565, 1422, 1458
HpyF10VI GCNNNNNNNGC 5 cut(s) 622, 698, 902, 1001, 1651
HpySE526I ACGT 3 cut(s) 565, 1422, 1458
Hsp92I GRCGYC 2 cut(s) 222, 915
Hsp92II CATG 9 cut(s) 489, 515, 636, 748, 847, 892, 1028, 1148, 1186
HspAI GCGC 3 cut(s) 499, 501, 523
Kzo9I GATC 3 cut(s) 476, 861, 1452
LmnI GCTCC 8 cut(s) 190, 206, 496, 737, 788, 814, 1024, 1462
Lsp1109I GCAGC 6 cut(s) 328, 532, 905, 1013, 1243, 1477
LweI GCATC 4 cut(s) 340, 344, 507, 596
MaeI CTAG 4 cut(s) 456, 693, 1211, 1388
MaeII ACGT 3 cut(s) 565, 1422, 1458
MalI GATC 3 cut(s) 478, 863, 1454
MboI GATC 3 cut(s) 476, 861, 1452
MboII GAAGA 5 cut(s) 315, 431, 434, 1107, 1277
MfeI CAATTG 1 cut(s) 620
MflI RGATCY 2 cut(s) 476, 861
MhlI GDGCHC 5 cut(s) 511, 785, 802, 1021, 1539
MluCI AATT 9 cut(s) 107, 130, 355, 389, 620, 723, 1032, 1121, 1139
MlyI GAGTC 4 cut(s) 976, 1378, 1421, 1630
MroXI GAANNNNTTC 1 cut(s) 723
MseI TTAA 6 cut(s) 275, 281, 404, 875, 1125, 1592
MspCI CTTAAG 1 cut(s) 280
MspI CCGG 1 cut(s) 602
MspR9I CCNGG 4 cut(s) 188, 494, 1200, 1558
MunI CAATTG 1 cut(s) 620
MvaI CCWGG 4 cut(s) 188, 494, 1200, 1558
MvnI CGCG 2 cut(s) 501, 503
MwoI GCNNNNNNNGC 5 cut(s) 622, 698, 902, 1001, 1651
NdeI CATATG 1 cut(s) 1600
NdeII GATC 3 cut(s) 476, 861, 1452
NlaIII CATG 9 cut(s) 489, 515, 636, 748, 847, 892, 1028, 1148, 1186
NlaIV GGNNCC 6 cut(s) 411, 491, 784, 816, 904, 1020
NmeAIII GCCGAG 1 cut(s) 1458
NmuCI GTSAC 8 cut(s) 179, 427, 566, 979, 1171, 1302, 1318, 1423
NspI RCATGY 4 cut(s) 515, 636, 847, 1186
PaeR7I CTCGAG 1 cut(s) 1479
PauI GCGCGC 1 cut(s) 499
PceI AGGCCT 1 cut(s) 1198
PciI ACATGT 1 cut(s) 511
PdmI GAANNNNTTC 1 cut(s) 723
PfeI GAWTC 3 cut(s) 155, 1340, 1369
PflMI CCANNNNNTGG 1 cut(s) 1280
PkrI GCNGC 6 cut(s) 318, 522, 895, 1003, 1233, 1467
PleI GAGTC 4 cut(s) 976, 1378, 1420, 1629
PpsI GAGTC 4 cut(s) 976, 1378, 1420, 1629
PscI ACATGT 1 cut(s) 511
PsiI TTATAA 1 cut(s) 1650
Psp124BI GAGCTC 1 cut(s) 1539
Psp6I CCWGG 4 cut(s) 186, 492, 1198, 1556
PspEI GGTNACC 1 cut(s) 427
PspGI CCWGG 4 cut(s) 186, 492, 1198, 1556
PspN4I GGNNCC 6 cut(s) 411, 491, 784, 816, 904, 1020
PspPI GGNCC 3 cut(s) 410, 903, 931
PstI CTGCAG 3 cut(s) 318, 923, 1233
PstNI CAGNNNCTG 1 cut(s) 1715
PsuI RGATCY 2 cut(s) 476, 861
PteI GCGCGC 1 cut(s) 499
RsaI GTAC 2 cut(s) 970, 1029
RsaNI GTAC 2 cut(s) 969, 1028
SacI GAGCTC 1 cut(s) 1539
SaqAI TTAA 6 cut(s) 275, 281, 404, 875, 1125, 1592
SatI GCNGC 6 cut(s) 317, 521, 894, 1002, 1232, 1466
Sau3AI GATC 3 cut(s) 476, 861, 1452
Sau96I GGNCC 3 cut(s) 410, 903, 931
SchI GAGTC 4 cut(s) 976, 1378, 1421, 1630
ScrFI CCNGG 4 cut(s) 188, 494, 1200, 1558
SduI GDGCHC 5 cut(s) 511, 785, 802, 1021, 1539
SfaNI GCATC 4 cut(s) 340, 344, 507, 596
SfcI CTRYAG 3 cut(s) 314, 919, 1229
Sfr274I CTCGAG 1 cut(s) 1479
SinI GGWCC 1 cut(s) 931
SlaI CTCGAG 1 cut(s) 1479
SmlI CTYRAG 2 cut(s) 280, 1479
SmoI CTYRAG 2 cut(s) 280, 1479
Sse9I AATT 9 cut(s) 107, 130, 355, 389, 620, 723, 1032, 1121, 1139
SseBI AGGCCT 1 cut(s) 1198
SsiI CCGC 3 cut(s) 432, 463, 503
SspMI CTAG 4 cut(s) 456, 693, 1211, 1388
SstI GAGCTC 1 cut(s) 1539
StuI AGGCCT 1 cut(s) 1198
StyD4I CCNGG 4 cut(s) 186, 492, 1198, 1556
StyI CCWWGG 1 cut(s) 1274
TaaI ACNGT 7 cut(s) 70, 260, 311, 555, 797, 935, 1101
TaiI ACGT 3 cut(s) 568, 1425, 1461
TaqI TCGA 3 cut(s) 304, 1044, 1480
TasI AATT 9 cut(s) 107, 130, 355, 389, 620, 723, 1032, 1121, 1139
TfiI GAWTC 3 cut(s) 155, 1340, 1369
Tru1I TTAA 6 cut(s) 275, 281, 404, 875, 1125, 1592
Tru9I TTAA 6 cut(s) 275, 281, 404, 875, 1125, 1592
TscAI CASTG 5 cut(s) 34, 73, 718, 1018, 1221
TseFI GTSAC 8 cut(s) 179, 427, 566, 979, 1171, 1302, 1318, 1423
TseI GCWGC 6 cut(s) 316, 520, 893, 1001, 1231, 1465
Tsp45I GTSAC 8 cut(s) 179, 427, 566, 979, 1171, 1302, 1318, 1423
TspDTI ATGAA 4 cut(s) 432, 597, 1133, 1266
TspRI CASTG 5 cut(s) 34, 73, 718, 1018, 1221
Van91I CCANNNNNTGG 1 cut(s) 1280
Vha464I CTTAAG 1 cut(s) 280
VneI GTGCAC 1 cut(s) 507
VpaK11BI GGWCC 1 cut(s) 931
XapI RAATTY 3 cut(s) 130, 1121, 1139
XbaI TCTAGA 1 cut(s) 1387
XceI RCATGY 4 cut(s) 515, 636, 847, 1186
XcmI CCANNNNNNNNNTGG 2 cut(s) 1538, 1564
XhoI CTCGAG 1 cut(s) 1479
XmiI GTMKAC 1 cut(s) 1624
XmnI GAANNNNTTC 1 cut(s) 723
XspI CTAG 4 cut(s) 456, 693, 1211, 1388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.