Rorug05G0159800

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000053
Physical Location & Seq
Forward (+)
14900523 .. 14907852
7330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0159800.1

Sequence Viewer

Length: 1533 bp
ATGCCCCAAGATCTTCCTGGGTTTTATTATGATGCGGAGAAGAATAGGTACTTCCCCAACAAGGGCCCGGTACCCGGTTCTCGTAGTACTGCTGCTGCTGCTGTGGCTTCTTCTAGTGCTCAGAAACTGAACCAGGAAGCCAACAAATGTAAGCTAACAAAAGTAAGAACTTCTAAGCTGCTTCAGGCTAGGGAGTTATATGGTAATGTCATTGCTTCCAGTAAAGGGAGGTGCAACTTCAAGGAGGAATTCCAAAAGGCACAAGCATCCCATCCTGTGGTTTGGAAGTATCAGGGAACAAACAAGCTAGGTGATGGTGCTTTGGAGCGGATAAACATTGATGTACAGACAGGAGAGGGTTTAACTGGTACAGATTTTCTCTTAGCAGGTGGCAGAAATGGCTCTTTAAGTTTATTTGAATTCGGAAAAGTCGGGCAGGGTTTTCATTATGGGATGAAATGCTTGCCAGATCTTGTCTGGCCTCTCTTAAACAAAAGTCAAGTAGAATGTAGAAAAGCGCCTGGGCACATATGGAGACCAGCTCAAAATACACCACGGATGTCATCGAGTATATCTTGTATAAAAACATTTGGCAAGCACAGCACAGAAAATGACGGTTCTAGCATCCAGAATGCTCTGGTATCTACGCTAGGAGCTGAGGACCGTGGTGGATCACTTTTTGTTATTAACCTTACTGAACTCTTAGATTTTCGCCCAAATAGATCTATCTTAAGGCGGAACATTGATGAAGTTGCGACTTTCAACTGCACTGTTTGGACAGCAGATTGTAGATCTGATGGGAATCGTGCAGTGATTGGAACCAATCTGGGAGCTGCTTTGGTAGATCTGGAAACTGGGGTGAAATCATGGCTGCTGCGTAGTAAAAGTGATGTTTTGGCACAACAACTTGTTCACTCGGGAAATGTTGTTTTATGTGGACTCAGAAATGGAGCAATTGTAACGGTTGATGTTCGTGAGAAACGGGAAGGATTTTCTGCAAGACATGCTAGTCATAGAATACGGCACTCACCTTTGGATAGCACTCCTGGAAAATCTAGTGAAAAATGGTTCAAGCTTTCTGGAAATATACATCCTTCTCATACCATAAAAATGCCTTCATCTATTGCATGTTTAGTATCGCTTCAGTTTGATGATCAGTACTTCTTGGCAAGCTCCATGGACGGAACGGTAAAGCTTTATGACCATCGTATAATCCAAAGAGGAGCTGTCCAAACTTATGAGGGGAATGTGAATTCCCATACTCGTTTGCAGCTTGGTGTTGATCCATCTGAGCGATTTTTTATATCAGGTGGAGAGGACTGCAATTTAAGACTTTGGAGTATAAAGTCAGGCGAACTGCTTTTTGAGGACAAGTTAACAAATACAGTCACCCCAACCGTGTGCTGGCAAAAAGCTGAAGCGTTTAAAGGATTGCAGGATGAAAGGCAAAGCTATGATAATGTTAAACATGATAGCTTGGGGGCTTGGCTTGGATCGGCAGAAGGAATATTTTACGTGGACTGGGAACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

510

Amino Acids

56.31

Weight (kDa)

8.82

Isoelectric Point (pI)

42.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 283 - 470 5.7e-06 THOC3 beta-propeller domain
Beta-prop_DCAF4 PF23761 373 - 454 5.2e-12 DDB1- and CUL4-associated factor 4 beta-propeller domain
Beta-prop_WDR5 PF25175 379 - 458 3.6e-07 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 377
AasI GACNNNNNNGTC 1 cut(s) 1008
Acc36I ACCTGC 1 cut(s) 377
Acc65I GGTACC 1 cut(s) 70
AccB1I GGYRCC 1 cut(s) 70
AccB7I CCANNNNNTGG 1 cut(s) 277
AccBSI CCGCTC 1 cut(s) 328
AciI CCGC 3 cut(s) 35, 328, 736
AclWI GGATC 3 cut(s) 679, 1277, 1501
AcsI RAATTY 3 cut(s) 248, 419, 1252
AcuI CTGAAG 3 cut(s) 167, 1127, 1437
AfaI GTAC 6 cut(s) 50, 72, 88, 345, 370, 1160
AfiI CCNNNNNNNGG 6 cut(s) 61, 62, 74, 225, 277, 1404
AflII CTTAAG 1 cut(s) 730
AgsI TTSAA 4 cut(s) 241, 419, 763, 1072
AjnI CCWGG 4 cut(s) 16, 132, 520, 1045
Alw21I GWGCWC 1 cut(s) 121
Alw26I GTCTC 1 cut(s) 529
AlwI GGATC 3 cut(s) 679, 1277, 1501
AlwNI CAGNNNCTG 1 cut(s) 127
Ama87I CYCGRG 1 cut(s) 916
AoxI GGCC 2 cut(s) 64, 479
ApaI GGGCCC 1 cut(s) 68
ApeKI GCWGC 8 cut(s) 92, 95, 98, 178, 833, 871, 874, 1270
ApoI RAATTY 3 cut(s) 248, 419, 1252
Asp718I GGTACC 1 cut(s) 70
AspLEI GCGC 1 cut(s) 520
AspS9I GGNCC 3 cut(s) 64, 65, 661
AsuC2I CCSGG 2 cut(s) 68, 75
AsuHPI GGTGA 4 cut(s) 323, 871, 1020, 1381
AvaI CYCGRG 1 cut(s) 916
AvaII GGWCC 1 cut(s) 661
BaeGI GKGCMC 2 cut(s) 68, 528
BanI GGYRCC 1 cut(s) 70
BanII GRGCYC 1 cut(s) 68
BarI GAAGNNNNNNTAC 2 cut(s) 32, 64
Bbv12I GWGCWC 1 cut(s) 121
BbvCI CCTCAGC 1 cut(s) 657
BbvI GCAGC 8 cut(s) 79, 82, 85, 165, 820, 858, 861, 1282
BccI CCATC 5 cut(s) 279, 308, 791, 1212, 1294
BceAI ACGGC 1 cut(s) 1037
BciT130I CCWGG 4 cut(s) 18, 134, 522, 1047
BclI TGATCA 1 cut(s) 1153
BcnI CCSGG 2 cut(s) 68, 75
BcoDI GTCTC 1 cut(s) 529
BfaI CTAG 7 cut(s) 114, 189, 308, 621, 650, 1008, 1056
BfoI RGCGCY 1 cut(s) 521
BfrI CTTAAG 1 cut(s) 730
BfuAI ACCTGC 1 cut(s) 377
BglII AGATCT 5 cut(s) 10, 469, 722, 791, 844
BisI GCNGC 8 cut(s) 93, 96, 99, 179, 834, 872, 875, 1271
BlsI GCNGC 8 cut(s) 94, 97, 100, 180, 835, 873, 876, 1272
BmcAI AGTACT 2 cut(s) 88, 1160
Bme1390I CCNGG 6 cut(s) 18, 68, 75, 134, 522, 1047
Bme18I GGWCC 1 cut(s) 661
BmeT110I CYCGRG 1 cut(s) 916
BmgT120I GGNCC 3 cut(s) 64, 65, 661
BmiI GGNNCC 3 cut(s) 66, 72, 820
BmrFI CCNGG 6 cut(s) 18, 68, 75, 134, 522, 1047
BmrI ACTGGG 2 cut(s) 864, 1531
BmsI GCATC 3 cut(s) 22, 275, 633
BmuI ACTGGG 2 cut(s) 864, 1531
BplI GAGNNNNNCTC 2 cut(s) 526, 558
Bpu10I CCTNAGC 1 cut(s) 657
BpuMI CCSGG 2 cut(s) 68, 75
BsaAI YACGTR 1 cut(s) 1516
BsaBI GATNNNNATC 1 cut(s) 801
BsaI GGTCTC 1 cut(s) 529
BsaJI CCNNGG 5 cut(s) 17, 521, 554, 664, 1176
BsaXI ACNNNNNCTCC 2 cut(s) 942, 972
Bsc4I CCNNNNNNNGG 6 cut(s) 61, 62, 74, 225, 277, 1404
Bse1I ACTGG 4 cut(s) 219, 370, 859, 1526
Bse3DI GCAATG 1 cut(s) 210
Bse8I GATNNNNATC 1 cut(s) 801
BseBI CCWGG 4 cut(s) 18, 134, 522, 1047
BseDI CCNNGG 5 cut(s) 17, 521, 554, 664, 1176
BseGI GGATG 7 cut(s) 266, 271, 459, 564, 624, 1090, 1444
BseJI GATNNNNATC 1 cut(s) 801
BseLI CCNNNNNNNGG 6 cut(s) 61, 62, 74, 225, 277, 1404
BseMI GCAATG 1 cut(s) 210
BseMII CTCAG 4 cut(s) 134, 648, 955, 1281
BseNI ACTGG 4 cut(s) 219, 370, 859, 1526
BseRI GAGGAG 1 cut(s) 1236
BseSI GKGCMC 2 cut(s) 68, 528
BseXI GCAGC 8 cut(s) 79, 82, 85, 165, 820, 858, 861, 1282
BsgI GTGCAG 2 cut(s) 751, 828
BshFI GGCC 2 cut(s) 66, 481
BshNI GGYRCC 1 cut(s) 70
BsiHKAI GWGCWC 1 cut(s) 121
BsiHKCI CYCGRG 1 cut(s) 916
BsiSI CCGG 2 cut(s) 68, 75
BslI CCNNNNNNNGG 6 cut(s) 61, 62, 74, 225, 277, 1404
BsmAI GTCTC 1 cut(s) 529
BsmI GAATGC 1 cut(s) 637
BsnI GGCC 2 cut(s) 66, 481
Bso31I GGTCTC 1 cut(s) 529
BsoBI CYCGRG 1 cut(s) 916
Bsp120I GGGCCC 1 cut(s) 64
Bsp1286I GDGCHC 3 cut(s) 68, 121, 528
Bsp1407I TGTACA 1 cut(s) 343
Bsp143I GATC 9 cut(s) 10, 469, 671, 722, 791, 844, 1153, 1282, 1493
Bsp19I CCATGG 1 cut(s) 1176
BspACI CCGC 3 cut(s) 35, 328, 736
BspANI GGCC 2 cut(s) 66, 481
BspCNI CTCAG 4 cut(s) 133, 649, 954, 1282
BspLI GGNNCC 3 cut(s) 66, 72, 820
BspMI ACCTGC 1 cut(s) 377
BspPI GGATC 3 cut(s) 679, 1277, 1501
BspT107I GGYRCC 1 cut(s) 70
BspTI CTTAAG 1 cut(s) 730
BspTNI GGTCTC 1 cut(s) 529
BsrBI CCGCTC 1 cut(s) 328
BsrDI GCAATG 1 cut(s) 210
BsrGI TGTACA 1 cut(s) 343
BsrI ACTGG 4 cut(s) 219, 370, 859, 1526
BssECI CCNNGG 5 cut(s) 17, 521, 554, 664, 1176
BssMI GATC 9 cut(s) 10, 469, 671, 722, 791, 844, 1153, 1282, 1493
BssT1I CCWWGG 1 cut(s) 1176
Bst2UI CCWGG 4 cut(s) 18, 134, 522, 1047
Bst4CI ACNGT 7 cut(s) 617, 665, 772, 964, 1189, 1387, 1399
BstAFI CTTAAG 1 cut(s) 730
BstAPI GCANNNNNTGC 1 cut(s) 1004
BstAUI TGTACA 1 cut(s) 343
BstBAI YACGTR 1 cut(s) 1516
BstC8I GCNNGC 4 cut(s) 464, 596, 1171, 1406
BstDEI CTNAG 7 cut(s) 120, 174, 382, 657, 703, 941, 1290
BstDSI CCRYGG 3 cut(s) 554, 664, 1176
BstF5I GGATG 7 cut(s) 266, 271, 459, 564, 624, 1090, 1444
BstH2I RGCGCY 1 cut(s) 521
BstHHI GCGC 1 cut(s) 520
BstKTI GATC 9 cut(s) 13, 472, 674, 725, 794, 847, 1156, 1285, 1496
BstMAI GTCTC 1 cut(s) 529
BstMBI GATC 9 cut(s) 10, 469, 671, 722, 791, 844, 1153, 1282, 1493
BstMWI GCNNNNNNNGC 5 cut(s) 98, 104, 399, 600, 1004
BstNI CCWGG 4 cut(s) 18, 134, 522, 1047
BstNSI RCATGY 2 cut(s) 1007, 1131
BstSCI CCNGG 6 cut(s) 16, 66, 73, 132, 520, 1045
BstSLI GKGCMC 2 cut(s) 68, 528
BstV1I GCAGC 8 cut(s) 79, 82, 85, 165, 820, 858, 861, 1282
BstX2I RGATCY 5 cut(s) 10, 469, 722, 791, 844
BstYI RGATCY 5 cut(s) 10, 469, 722, 791, 844
BsuRI GGCC 2 cut(s) 66, 481
BtgI CCRYGG 3 cut(s) 554, 664, 1176
BtsCI GGATG 7 cut(s) 266, 271, 459, 564, 624, 1090, 1444
BtsI GCAGTG 1 cut(s) 816
BtsIMutI CAGTG 2 cut(s) 768, 816
BveI ACCTGC 1 cut(s) 377
Cac8I GCNNGC 4 cut(s) 464, 596, 1171, 1406
CaiI CAGNNNCTG 1 cut(s) 127
CfoI GCGC 1 cut(s) 520
Cfr13I GGNCC 3 cut(s) 64, 65, 661
Csp6I GTAC 6 cut(s) 49, 71, 87, 344, 369, 1159
CviAII CATG 5 cut(s) 867, 1004, 1128, 1177, 1469
CviQI GTAC 6 cut(s) 49, 71, 87, 344, 369, 1159
DdeI CTNAG 7 cut(s) 120, 174, 382, 657, 703, 941, 1290
DpnI GATC 9 cut(s) 12, 471, 673, 724, 793, 846, 1155, 1284, 1495
DpnII GATC 9 cut(s) 10, 469, 671, 722, 791, 844, 1153, 1282, 1493
DraI TTTAAA 1 cut(s) 1426
DrdI GACNNNNNNGTC 1 cut(s) 1008
DseDI GACNNNNNNGTC 1 cut(s) 1008
EciI GGCGGA 1 cut(s) 751
Eco130I CCWWGG 1 cut(s) 1176
Eco24I GRGCYC 1 cut(s) 68
Eco31I GGTCTC 1 cut(s) 529
Eco47I GGWCC 1 cut(s) 661
Eco57I CTGAAG 3 cut(s) 167, 1127, 1437
Eco88I CYCGRG 1 cut(s) 916
EcoO109I RGGNCCY 1 cut(s) 64
EcoRI GAATTC 3 cut(s) 248, 419, 1252
EcoRII CCWGG 4 cut(s) 16, 132, 520, 1045
EcoT14I CCWWGG 1 cut(s) 1176
EcoT38I GRGCYC 1 cut(s) 68
ErhI CCWWGG 1 cut(s) 1176
FaeI CATG 5 cut(s) 870, 1007, 1131, 1180, 1472
FatI CATG 5 cut(s) 866, 1003, 1127, 1176, 1468
FauNDI CATATG 1 cut(s) 530
FbaI TGATCA 1 cut(s) 1153
Fnu4HI GCNGC 8 cut(s) 93, 96, 99, 179, 834, 872, 875, 1271
FokI GGATG 7 cut(s) 253, 258, 466, 571, 611, 1077, 1451
FriOI GRGCYC 1 cut(s) 68
Fsp4HI GCNGC 8 cut(s) 93, 96, 99, 179, 834, 872, 875, 1271
FspBI CTAG 7 cut(s) 114, 189, 308, 621, 650, 1008, 1056
GlaI GCGC 1 cut(s) 519
GluI GCNGC 8 cut(s) 93, 96, 99, 179, 834, 872, 875, 1271
HaeII RGCGCY 1 cut(s) 521
HaeIII GGCC 2 cut(s) 66, 481
HapII CCGG 2 cut(s) 68, 75
HhaI GCGC 1 cut(s) 520
Hin1II CATG 5 cut(s) 870, 1007, 1131, 1180, 1472
Hin6I GCGC 1 cut(s) 518
HinP1I GCGC 1 cut(s) 518
HincII GTYRAC 1 cut(s) 1377
HindII GTYRAC 1 cut(s) 1377
HindIII AAGCTT 2 cut(s) 1073, 1193
HinfI GANTC 2 cut(s) 802, 939
HpaI GTTAAC 1 cut(s) 1377
HpaII CCGG 2 cut(s) 68, 75
HphI GGTGA 4 cut(s) 323, 871, 1020, 1381
Hpy166II GTNNAC 4 cut(s) 913, 938, 1377, 1519
Hpy188I TCNGA 5 cut(s) 123, 425, 796, 944, 1291
Hpy188III TCNNGA 5 cut(s) 628, 848, 918, 974, 1080
Hpy8I GTNNAC 4 cut(s) 913, 938, 1377, 1519
HpyAV CCTTC 4 cut(s) 980, 1104, 1125, 1496
HpyCH4III ACNGT 7 cut(s) 617, 665, 772, 964, 1189, 1387, 1399
HpyCH4IV ACGT 1 cut(s) 1515
HpyCH4V TGCA 8 cut(s) 234, 768, 809, 998, 1127, 1270, 1323, 1435
HpyF10VI GCNNNNNNNGC 5 cut(s) 98, 104, 399, 600, 1004
HpyF3I CTNAG 7 cut(s) 120, 174, 382, 657, 703, 941, 1290
HpySE526I ACGT 1 cut(s) 1515
Hsp92II CATG 5 cut(s) 870, 1007, 1131, 1180, 1472
HspAI GCGC 1 cut(s) 518
KpnI GGTACC 1 cut(s) 74
Ksp22I TGATCA 1 cut(s) 1153
KspAI GTTAAC 1 cut(s) 1377
Kzo9I GATC 9 cut(s) 10, 469, 671, 722, 791, 844, 1153, 1282, 1493
LmnI GCTCC 6 cut(s) 325, 653, 830, 950, 1178, 1223
Lsp1109I GCAGC 8 cut(s) 79, 82, 85, 165, 820, 858, 861, 1282
LweI GCATC 3 cut(s) 22, 275, 633
MaeI CTAG 7 cut(s) 114, 189, 308, 621, 650, 1008, 1056
MaeII ACGT 1 cut(s) 1515
MaeIII GTNAC 2 cut(s) 958, 1387
MalI GATC 9 cut(s) 12, 471, 673, 724, 793, 846, 1155, 1284, 1495
MbiI CCGCTC 1 cut(s) 328
MboI GATC 9 cut(s) 10, 469, 671, 722, 791, 844, 1153, 1282, 1493
MboII GAAGA 3 cut(s) 5, 52, 102
MfeI CAATTG 1 cut(s) 954
MflI RGATCY 5 cut(s) 10, 469, 722, 791, 844
MhlI GDGCHC 3 cut(s) 68, 121, 528
MluCI AATT 5 cut(s) 248, 419, 954, 1252, 1324
MlyI GAGTC 1 cut(s) 933
MnlI CCTC 9 cut(s) 222, 238, 349, 492, 652, 1214, 1234, 1309, 1360
MseI TTAA 9 cut(s) 362, 407, 488, 687, 731, 1328, 1376, 1425, 1464
MslI CAYNNNNRTG 1 cut(s) 1109
MspCI CTTAAG 1 cut(s) 730
MspI CCGG 2 cut(s) 68, 75
MspR9I CCNGG 6 cut(s) 18, 68, 75, 134, 522, 1047
MunI CAATTG 1 cut(s) 954
Mva1269I GAATGC 1 cut(s) 637
MvaI CCWGG 4 cut(s) 18, 134, 522, 1047
MwoI GCNNNNNNNGC 5 cut(s) 98, 104, 399, 600, 1004
NciI CCSGG 2 cut(s) 68, 75
NcoI CCATGG 1 cut(s) 1176
NdeI CATATG 1 cut(s) 530
NdeII GATC 9 cut(s) 10, 469, 671, 722, 791, 844, 1153, 1282, 1493
NlaIII CATG 5 cut(s) 870, 1007, 1131, 1180, 1472
NlaIV GGNNCC 3 cut(s) 66, 72, 820
NmuCI GTSAC 1 cut(s) 1387
NspI RCATGY 2 cut(s) 1007, 1131
PaqCI CACCTGC 1 cut(s) 377
PctI GAATGC 1 cut(s) 637
PfeI GAWTC 1 cut(s) 802
PflMI CCANNNNNTGG 1 cut(s) 277
PfoI TCCNGGA 1 cut(s) 1045
PkrI GCNGC 8 cut(s) 94, 97, 100, 180, 835, 873, 876, 1272
PleI GAGTC 1 cut(s) 933
PpsI GAGTC 1 cut(s) 933
Ppu21I YACGTR 1 cut(s) 1516
Psp6I CCWGG 4 cut(s) 16, 132, 520, 1045
PspGI CCWGG 4 cut(s) 16, 132, 520, 1045
PspN4I GGNNCC 3 cut(s) 66, 72, 820
PspOMI GGGCCC 1 cut(s) 64
PspPI GGNCC 3 cut(s) 64, 65, 661
PstNI CAGNNNCTG 1 cut(s) 127
PsuI RGATCY 5 cut(s) 10, 469, 722, 791, 844
RsaI GTAC 6 cut(s) 50, 72, 88, 345, 370, 1160
RsaNI GTAC 6 cut(s) 49, 71, 87, 344, 369, 1159
RseI CAYNNNNRTG 1 cut(s) 1109
SaqAI TTAA 9 cut(s) 362, 407, 488, 687, 731, 1328, 1376, 1425, 1464
SatI GCNGC 8 cut(s) 93, 96, 99, 179, 834, 872, 875, 1271
Sau3AI GATC 9 cut(s) 10, 469, 671, 722, 791, 844, 1153, 1282, 1493
Sau96I GGNCC 3 cut(s) 64, 65, 661
ScaI AGTACT 2 cut(s) 88, 1160
SchI GAGTC 1 cut(s) 933
ScrFI CCNGG 6 cut(s) 18, 68, 75, 134, 522, 1047
SduI GDGCHC 3 cut(s) 68, 121, 528
SfaNI GCATC 3 cut(s) 22, 275, 633
SinI GGWCC 1 cut(s) 661
SmiMI CAYNNNNRTG 1 cut(s) 1109
SmlI CTYRAG 1 cut(s) 730
SmoI CTYRAG 1 cut(s) 730
Sse9I AATT 5 cut(s) 248, 419, 954, 1252, 1324
SsiI CCGC 3 cut(s) 35, 328, 736
SspI AATATT 1 cut(s) 1509
SspMI CTAG 7 cut(s) 114, 189, 308, 621, 650, 1008, 1056
StyD4I CCNGG 6 cut(s) 16, 66, 73, 132, 520, 1045
StyI CCWWGG 1 cut(s) 1176
TaaI ACNGT 7 cut(s) 617, 665, 772, 964, 1189, 1387, 1399
TaiI ACGT 1 cut(s) 1518
TaqI TCGA 1 cut(s) 566
TasI AATT 5 cut(s) 248, 419, 954, 1252, 1324
TatI WGTACW 3 cut(s) 86, 343, 1158
TfiI GAWTC 1 cut(s) 802
Tru1I TTAA 9 cut(s) 362, 407, 488, 687, 731, 1328, 1376, 1425, 1464
Tru9I TTAA 9 cut(s) 362, 407, 488, 687, 731, 1328, 1376, 1425, 1464
TscAI CASTG 2 cut(s) 775, 816
TseFI GTSAC 1 cut(s) 1387
TseI GCWGC 8 cut(s) 92, 95, 98, 178, 833, 871, 874, 1270
Tsp45I GTSAC 1 cut(s) 1387
TspDTI ATGAA 5 cut(s) 434, 470, 762, 1107, 1455
TspGWI ACGGA 2 cut(s) 571, 1197
TspRI CASTG 2 cut(s) 775, 816
Van91I CCANNNNNTGG 1 cut(s) 277
Vha464I CTTAAG 1 cut(s) 730
VpaK11BI GGWCC 1 cut(s) 661
XapI RAATTY 3 cut(s) 248, 419, 1252
XceI RCATGY 2 cut(s) 1007, 1131
XcmI CCANNNNNNNNNTGG 3 cut(s) 14, 474, 1401
XspI CTAG 7 cut(s) 114, 189, 308, 621, 650, 1008, 1056
ZrmI AGTACT 2 cut(s) 88, 1160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.