Rw5G036500

(-)-germacrene D synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000053
Physical Location & Seq
Reverse (-)
64047837 .. 64051441
3605 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G036500.1

Sequence Viewer

Length: 1677 bp
ATGCACAGGGGAATAAGAGGAGGGTTGTCCTCATTACGTTGTATGCTAAGTTCCCATATGAACTTTATATATATATATATATATATACACACACACACAAACATACAACGCCCTTCTAATGAGAGATCCATTTTTTTGCAACGAGTCCAAGAACTTAAAAGAGAAGTGAAAATGATCCTCATGGTGCCAGCAAAAGAGCCTTCACAAAAATTGAACTTAGTTGATGACATTCAGCGCCTAGGCGTATCTTACCATTTTGAAAATGAGATCGATGAAATTCTGAAACAAATTTACCAGACTACTTATGGTAGTGATGTGGAATATGATGATGACCTTTACACTACTGCTCTTTGTTTTCGGCTACTCAGACAACACGGTTATAATGTTTCAAGTGATATGTTCAACAAGTACTTCAATGAGGGTAATGATGAGAAATTTAAGGAATCTCTTTTGAGTGATGTATCAGGACTATTAAGTTTATACGAAGCCACCCATCTTAGGATACACGGTGAAGACATACTCGAAGAGGCACTAGCCTTCACCACCACACATCTCGAGTCGATGAAAGATAGTTTAAGCCCTCCACTTTCGAAACAAGTAGCCCATTCCTTAAATCAGCCACTACATAAGGGCATCACCAGAGTGGAAGCAAGATATTACTTATCAATCTACCACGAATGCAATTCACATAATGAAACTCTCTTGACTTTTGCGAAGTTGGATTTCAACCTGTTGCAGCAGCTCCATCAAAACGAACTTTGTGAAATTACAAGGTGGTGGAAGGACTTGGACGTGAGAAACGAGCTGCTGTTTACAAGAGACAGAGTTACCGAGGTTTACTTCTGCTGGGCTTCATCAGTCTGCTTTGAACCTGAATATTCCTTTGCTAGGAGGACATTATGCAAAGTTACTGCCCTGGCATCCATTCTCGATGACATTTATGATTTGTATGGCACATTCGAAGAACTAGAGCTCTTTACTGAAGCTATTGAGAGGTGGGATATATGTTTCATGGATGCACTGCCAAACTATATGAAAGTCTATTATAAGGCATTGTTAGACGTGTATACTGAAGTTGAAGAAGAGCTTGCAAAGGAGGGAAAGTTGTATCGAATCCACTATGTCATAGAAGCAATGAAAAAGCTAGCTCAAGGTTACTTCCTTGAGGCTCAATGGTTCCACCAGAATTACATACCAACAATGGATGAATATATGGACCTATCATTACTGACCTCAGGCGCCCCACTGCTAGCAACCACCGCTTTTGTTGGAATGGGAGATACTGCTACAGAAGATTCCTTTGATTGGCTAGCCACTTATCCCAGGGCTCTGAAAGGGTCTGCAATAGTTTGCCGACTTCTTAATGACATAGTGTCCCACAAGTTTGAGCAAAAAAGAGGACATGTTGCGTCAGCTGTGGAGTGCTACATGAAAGAATTTGGTGCCACAGAAGAAGAAGCAATAATCGAATTGCGTAGACAAGTGAGTGATGCGTGGAAGGACATGAACAAATCGTTTCTCCTCCCTAATGCTGTTTCGAGGCCACTGCTAACTCGAATTTTCAATATGGCATGCGGCACAGATGCTGTTTACAAGTATGGAGATTGTTACACTACACCGCATGATATGATCAAGGATCTTATAGCTTCTACACTTATCCAACAAGTGCCTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

558

Amino Acids

64.86

Weight (kDa)

5.26

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 39 - 204 2e-58 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 261 - 501 2.3e-93 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 302 - 501 4.1e-64 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 381, 1047
AccB1I GGYRCC 3 cut(s) 184, 1238, 1442
AccI GTMKAC 2 cut(s) 1067, 1477
AciI CCGC 3 cut(s) 1260, 1575, 1619
AclWI GGATC 3 cut(s) 120, 169, 1644
AcsI RAATTY 5 cut(s) 276, 288, 434, 1436, 1557
AcuI CTGAAG 2 cut(s) 1002, 1092
AcyI GRCGYC 1 cut(s) 1239
AfaI GTAC 1 cut(s) 410
AfiI CCNNNNNNNGG 3 cut(s) 498, 888, 1305
AflIII ACRYGT 2 cut(s) 1062, 1402
AgsI TTSAA 9 cut(s) 214, 260, 390, 403, 415, 727, 869, 1079, 1564
AhdI GACNNNNNGTC 1 cut(s) 1372
AjiI CACGTC 2 cut(s) 793, 1063
AjnI CCWGG 2 cut(s) 915, 1322
AleI CACNNNNGTG 1 cut(s) 641
AluBI AGCT 9 cut(s) 742, 805, 973, 986, 1087, 1144, 1148, 1415, 1646
AluI AGCT 9 cut(s) 742, 805, 973, 986, 1087, 1144, 1148, 1415, 1646
Alw21I GWGCWC 1 cut(s) 975
Alw26I GTCTC 1 cut(s) 813
AlwI GGATC 3 cut(s) 120, 169, 1644
AlwNI CAGNNNCTG 1 cut(s) 1586
Ama87I CYCGRG 1 cut(s) 554
AoxI GGCC 1 cut(s) 1541
ApeKI GCWGC 3 cut(s) 736, 739, 805
ApoI RAATTY 5 cut(s) 276, 288, 434, 1436, 1557
AspA2I CCTAGG 1 cut(s) 238
AspLEI GCGC 2 cut(s) 237, 1241
AspS9I GGNCC 1 cut(s) 1216
AsuHPI GGTGA 3 cut(s) 521, 532, 628
AsuII TTCGAA 2 cut(s) 590, 960
AsuNHI GCTAGC 3 cut(s) 1144, 1249, 1309
AvaI CYCGRG 1 cut(s) 554
AvaII GGWCC 1 cut(s) 1216
AvrII CCTAGG 1 cut(s) 238
AxyI CCTNAGG 1 cut(s) 1234
BanI GGYRCC 3 cut(s) 184, 1238, 1442
BanII GRGCYC 2 cut(s) 975, 1330
BbsI GAAGAC 1 cut(s) 519
Bbv12I GWGCWC 1 cut(s) 975
BbvI GCAGC 3 cut(s) 748, 751, 792
BccI CCATC 2 cut(s) 501, 753
BcgI CGANNNNNNTGC 2 cut(s) 1528, 1562
BciT130I CCWGG 2 cut(s) 917, 1324
BciVI GTATCC 1 cut(s) 495
BclI TGATCA 1 cut(s) 1629
BcoDI GTCTC 1 cut(s) 813
BfaI CTAG 7 cut(s) 239, 533, 888, 968, 1145, 1250, 1310
BfmI CTRYAG 1 cut(s) 1287
BfoI RGCGCY 2 cut(s) 238, 1242
BfuI GTATCC 1 cut(s) 495
BisI GCNGC 4 cut(s) 737, 740, 806, 1576
BlnI CCTAGG 1 cut(s) 238
BlsI GCNGC 4 cut(s) 738, 741, 807, 1577
BmcAI AGTACT 1 cut(s) 410
Bme1390I CCNGG 2 cut(s) 917, 1324
Bme18I GGWCC 1 cut(s) 1216
BmeRI GACNNNNNGTC 1 cut(s) 1372
BmeT110I CYCGRG 1 cut(s) 554
BmgBI CACGTC 2 cut(s) 793, 1063
BmgT120I GGNCC 1 cut(s) 1216
BmiI GGNNCC 4 cut(s) 186, 1178, 1240, 1444
BmrFI CCNGG 2 cut(s) 917, 1324
BmsI GCATC 5 cut(s) 642, 929, 1006, 1480, 1573
BmtI GCTAGC 3 cut(s) 1148, 1253, 1313
BpiI GAAGAC 1 cut(s) 519
Bpu14I TTCGAA 2 cut(s) 590, 960
BpuEI CTTGAG 2 cut(s) 1134, 1184
Bsa29I ATCGAT 1 cut(s) 270
BsaHI GRCGYC 1 cut(s) 1239
BsaJI CCNNGG 5 cut(s) 238, 831, 915, 1322, 1323
BsaXI ACNNNNNCTCC 2 cut(s) 1088, 1118
Bsc4I CCNNNNNNNGG 3 cut(s) 498, 888, 1305
Bse21I CCTNAGG 1 cut(s) 1234
Bse3DI GCAATG 1 cut(s) 1140
BseBI CCWGG 2 cut(s) 917, 1324
BseCI ATCGAT 1 cut(s) 270
BseDI CCNNGG 5 cut(s) 238, 831, 915, 1322, 1323
BseGI GGATG 3 cut(s) 920, 1021, 1210
BseLI CCNNNNNNNGG 3 cut(s) 498, 888, 1305
BseMI GCAATG 1 cut(s) 1140
BseMII CTCAG 2 cut(s) 379, 1248
BseRI GAGGAG 2 cut(s) 33, 1511
BseXI GCAGC 3 cut(s) 748, 751, 792
BseYI CCCAGC 1 cut(s) 846
BshFI GGCC 1 cut(s) 1543
BshNI GGYRCC 3 cut(s) 184, 1238, 1442
BshVI ATCGAT 1 cut(s) 270
BsiHKAI GWGCWC 1 cut(s) 975
BsiHKCI CYCGRG 1 cut(s) 554
BslFI GGGAC 1 cut(s) 1360
BslI CCNNNNNNNGG 3 cut(s) 498, 888, 1305
BsmAI GTCTC 1 cut(s) 813
BsmFI GGGAC 1 cut(s) 1360
BsmI GAATGC 1 cut(s) 683
BsnI GGCC 1 cut(s) 1543
BsoBI CYCGRG 1 cut(s) 554
Bsp119I TTCGAA 2 cut(s) 590, 960
Bsp1286I GDGCHC 2 cut(s) 975, 1330
Bsp143I GATC 5 cut(s) 125, 174, 267, 1629, 1636
BspACI CCGC 3 cut(s) 1260, 1575, 1619
BspANI GGCC 1 cut(s) 1543
BspCNI CTCAG 2 cut(s) 378, 1247
BspDI ATCGAT 1 cut(s) 270
BspLI GGNNCC 4 cut(s) 186, 1178, 1240, 1444
BspOI GCTAGC 3 cut(s) 1148, 1253, 1313
BspPI GGATC 3 cut(s) 120, 169, 1644
BspQI GCTCTTC 1 cut(s) 1077
BspT104I TTCGAA 2 cut(s) 590, 960
BspT107I GGYRCC 3 cut(s) 184, 1238, 1442
BsrDI GCAATG 1 cut(s) 1140
BssECI CCNNGG 5 cut(s) 238, 831, 915, 1322, 1323
BssMI GATC 5 cut(s) 125, 174, 267, 1629, 1636
BssNAI GTATAC 1 cut(s) 1068
BssNI GRCGYC 1 cut(s) 1239
BssT1I CCWWGG 1 cut(s) 238
Bst1107I GTATAC 1 cut(s) 1068
Bst2UI CCWGG 2 cut(s) 917, 1324
Bst4CI ACNGT 2 cut(s) 377, 509
Bst6I CTCTTC 2 cut(s) 519, 1077
BstACI GRCGYC 1 cut(s) 1239
BstBI TTCGAA 2 cut(s) 590, 960
BstC8I GCNNGC 6 cut(s) 189, 1089, 1146, 1251, 1311, 1573
BstDEI CTNAG 5 cut(s) 47, 217, 365, 497, 1234
BstENI CCTNNNNNAGG 1 cut(s) 886
BstF5I GGATG 3 cut(s) 920, 1021, 1210
BstH2I RGCGCY 2 cut(s) 238, 1242
BstHHI GCGC 2 cut(s) 237, 1241
BstKTI GATC 5 cut(s) 128, 177, 270, 1632, 1639
BstMAI GTCTC 1 cut(s) 813
BstMBI GATC 5 cut(s) 125, 174, 267, 1629, 1636
BstMWI GCNNNNNNNGC 1 cut(s) 1259
BstNI CCWGG 2 cut(s) 917, 1324
BstNSI RCATGY 2 cut(s) 1406, 1575
BstSCI CCNGG 2 cut(s) 915, 1322
BstSFI CTRYAG 1 cut(s) 1287
BstV1I GCAGC 3 cut(s) 748, 751, 792
BstV2I GAAGAC 1 cut(s) 519
BstX2I RGATCY 2 cut(s) 125, 1636
BstYI RGATCY 2 cut(s) 125, 1636
BstZ17I GTATAC 1 cut(s) 1068
Bsu15I ATCGAT 1 cut(s) 270
Bsu36I CCTNAGG 1 cut(s) 1234
BsuI GTATCC 1 cut(s) 495
BsuRI GGCC 1 cut(s) 1543
BsuTUI ATCGAT 1 cut(s) 270
BtrI CACGTC 2 cut(s) 793, 1063
BtsCI GGATG 3 cut(s) 920, 1021, 1210
BtsI GCAGTG 3 cut(s) 1019, 1244, 1544
BtsIMutI CAGTG 3 cut(s) 1019, 1244, 1544
Cac8I GCNNGC 6 cut(s) 189, 1089, 1146, 1251, 1311, 1573
CaiI CAGNNNCTG 1 cut(s) 1586
CfoI GCGC 2 cut(s) 237, 1241
Cfr13I GGNCC 1 cut(s) 1216
ClaI ATCGAT 1 cut(s) 270
CseI GACGC 1 cut(s) 1398
Csp6I GTAC 1 cut(s) 409
CspCI CAANNNNNGTGG 2 cut(s) 1246, 1281
CviAII CATG 7 cut(s) 181, 1012, 1403, 1429, 1504, 1572, 1622
CviQI GTAC 1 cut(s) 409
DdeI CTNAG 5 cut(s) 47, 217, 365, 497, 1234
DinI GGCGCC 1 cut(s) 1240
DpnI GATC 5 cut(s) 127, 176, 269, 1631, 1638
DpnII GATC 5 cut(s) 125, 174, 267, 1629, 1636
DriI GACNNNNNGTC 1 cut(s) 1372
Eam1104I CTCTTC 2 cut(s) 519, 1077
Eam1105I GACNNNNNGTC 1 cut(s) 1372
EarI CTCTTC 2 cut(s) 519, 1077
Ecl136II GAGCTC 1 cut(s) 973
Eco130I CCWWGG 1 cut(s) 238
Eco24I GRGCYC 2 cut(s) 975, 1330
Eco47I GGWCC 1 cut(s) 1216
Eco53kI GAGCTC 1 cut(s) 973
Eco57I CTGAAG 2 cut(s) 1002, 1092
Eco81I CCTNAGG 1 cut(s) 1234
Eco88I CYCGRG 1 cut(s) 554
EcoICRI GAGCTC 1 cut(s) 973
EcoNI CCTNNNNNAGG 1 cut(s) 886
EcoRII CCWGG 2 cut(s) 915, 1322
EcoT14I CCWWGG 1 cut(s) 238
EcoT38I GRGCYC 2 cut(s) 975, 1330
EgeI GGCGCC 1 cut(s) 1240
EheI GGCGCC 1 cut(s) 1240
ErhI CCWWGG 1 cut(s) 238
FaeI CATG 7 cut(s) 184, 1015, 1406, 1432, 1507, 1575, 1625
FalI AAGNNNNNCTT 2 cut(s) 1071, 1103
FaqI GGGAC 1 cut(s) 1360
FatI CATG 7 cut(s) 180, 1011, 1402, 1428, 1503, 1571, 1621
FauNDI CATATG 1 cut(s) 57
FbaI TGATCA 1 cut(s) 1629
FblI GTMKAC 2 cut(s) 1067, 1477
Fnu4HI GCNGC 4 cut(s) 737, 740, 806, 1576
FokI GGATG 3 cut(s) 907, 1028, 1217
FriOI GRGCYC 2 cut(s) 975, 1330
Fsp4HI GCNGC 4 cut(s) 737, 740, 806, 1576
FspBI CTAG 7 cut(s) 239, 533, 888, 968, 1145, 1250, 1310
GlaI GCGC 2 cut(s) 236, 1240
GluI GCNGC 4 cut(s) 737, 740, 806, 1576
GsaI CCCAGC 1 cut(s) 850
HaeII RGCGCY 2 cut(s) 238, 1242
HaeIII GGCC 1 cut(s) 1543
HgaI GACGC 1 cut(s) 1398
HhaI GCGC 2 cut(s) 237, 1241
Hin1I GRCGYC 1 cut(s) 1239
Hin1II CATG 7 cut(s) 184, 1015, 1406, 1432, 1507, 1575, 1625
Hin6I GCGC 2 cut(s) 235, 1239
HinP1I GCGC 2 cut(s) 235, 1239
HinfI GANTC 5 cut(s) 144, 443, 557, 1113, 1295
HphI GGTGA 3 cut(s) 521, 532, 628
Hpy166II GTNNAC 5 cut(s) 813, 838, 1068, 1478, 1591
Hpy188I TCNGA 3 cut(s) 282, 368, 1332
Hpy188III TCNNGA 4 cut(s) 465, 554, 703, 929
Hpy8I GTNNAC 5 cut(s) 813, 838, 1068, 1478, 1591
HpyAV CCTTC 5 cut(s) 123, 210, 547, 775, 1492
HpyCH4III ACNGT 2 cut(s) 377, 509
HpyCH4IV ACGT 3 cut(s) 37, 792, 1062
HpyCH4V TGCA 8 cut(s) 4, 139, 681, 736, 903, 1019, 1091, 1343
HpyF10VI GCNNNNNNNGC 1 cut(s) 1259
HpyF3I CTNAG 5 cut(s) 47, 217, 365, 497, 1234
HpySE526I ACGT 3 cut(s) 37, 792, 1062
Hsp92I GRCGYC 1 cut(s) 1239
Hsp92II CATG 7 cut(s) 184, 1015, 1406, 1432, 1507, 1575, 1625
HspAI GCGC 2 cut(s) 235, 1239
KasI GGCGCC 1 cut(s) 1238
Ksp22I TGATCA 1 cut(s) 1629
Kzo9I GATC 5 cut(s) 125, 174, 267, 1629, 1636
LguI GCTCTTC 1 cut(s) 1077
LmnI GCTCC 1 cut(s) 747
Lsp1109I GCAGC 3 cut(s) 748, 751, 792
LweI GCATC 5 cut(s) 642, 929, 1006, 1480, 1573
MaeI CTAG 7 cut(s) 239, 533, 888, 968, 1145, 1250, 1310
MaeII ACGT 3 cut(s) 37, 792, 1062
MaeIII GTNAC 4 cut(s) 826, 907, 1154, 1607
MalI GATC 5 cut(s) 127, 176, 269, 1631, 1638
MboI GATC 5 cut(s) 125, 174, 267, 1629, 1636
MboII GAAGA 8 cut(s) 524, 536, 974, 1091, 1094, 1304, 1463, 1466
MflI RGATCY 2 cut(s) 125, 1636
MhlI GDGCHC 2 cut(s) 975, 1330
Mly113I GGCGCC 1 cut(s) 1239
MlyI GAGTC 2 cut(s) 153, 566
MmeI TCCRAC 2 cut(s) 699, 1249
MseI TTAA 6 cut(s) 156, 438, 473, 575, 611, 1362
MslI CAYNNNNRTG 1 cut(s) 641
MspA1I CMGCKG 1 cut(s) 1415
MspR9I CCNGG 2 cut(s) 917, 1324
Mva1269I GAATGC 1 cut(s) 683
MvaI CCWGG 2 cut(s) 917, 1324
MwoI GCNNNNNNNGC 1 cut(s) 1259
NarI GGCGCC 1 cut(s) 1239
NdeI CATATG 1 cut(s) 57
NdeII GATC 5 cut(s) 125, 174, 267, 1629, 1636
NheI GCTAGC 3 cut(s) 1144, 1249, 1309
NlaIII CATG 7 cut(s) 184, 1015, 1406, 1432, 1507, 1575, 1625
NlaIV GGNNCC 4 cut(s) 186, 1178, 1240, 1444
NspI RCATGY 2 cut(s) 1406, 1575
NspV TTCGAA 2 cut(s) 590, 960
OliI CACNNNNGTG 1 cut(s) 641
PaeI GCATGC 1 cut(s) 1575
PaeR7I CTCGAG 1 cut(s) 554
PasI CCCWGGG 1 cut(s) 1323
PciI ACATGT 1 cut(s) 1402
PciSI GCTCTTC 1 cut(s) 1077
PcsI WCGNNNNNNNCGW 1 cut(s) 798
PctI GAATGC 1 cut(s) 683
PfeI GAWTC 3 cut(s) 443, 1113, 1295
PkrI GCNGC 4 cut(s) 738, 741, 807, 1577
PleI GAGTC 2 cut(s) 152, 565
PluTI GGCGCC 1 cut(s) 1242
PpsI GAGTC 2 cut(s) 152, 565
PscI ACATGT 1 cut(s) 1402
PsiI TTATAA 2 cut(s) 381, 1047
Psp124BI GAGCTC 1 cut(s) 975
Psp6I CCWGG 2 cut(s) 915, 1322
PspFI CCCAGC 1 cut(s) 846
PspGI CCWGG 2 cut(s) 915, 1322
PspN4I GGNNCC 4 cut(s) 186, 1178, 1240, 1444
PspPI GGNCC 1 cut(s) 1216
PsrI GAACNNNNNNTAC 2 cut(s) 34, 66
PstNI CAGNNNCTG 1 cut(s) 1586
PsuI RGATCY 2 cut(s) 125, 1636
PvuII CAGCTG 1 cut(s) 1415
RsaI GTAC 1 cut(s) 410
RsaNI GTAC 1 cut(s) 409
RseI CAYNNNNRTG 1 cut(s) 641
SacI GAGCTC 1 cut(s) 975
SapI GCTCTTC 1 cut(s) 1077
SaqAI TTAA 6 cut(s) 156, 438, 473, 575, 611, 1362
SatI GCNGC 4 cut(s) 737, 740, 806, 1576
Sau3AI GATC 5 cut(s) 125, 174, 267, 1629, 1636
Sau96I GGNCC 1 cut(s) 1216
ScaI AGTACT 1 cut(s) 410
SchI GAGTC 2 cut(s) 153, 566
ScrFI CCNGG 2 cut(s) 917, 1324
SduI GDGCHC 2 cut(s) 975, 1330
SfaNI GCATC 5 cut(s) 642, 929, 1006, 1480, 1573
SfcI CTRYAG 1 cut(s) 1287
SfoI GGCGCC 1 cut(s) 1240
Sfr274I CTCGAG 1 cut(s) 554
SfuI TTCGAA 2 cut(s) 590, 960
SinI GGWCC 1 cut(s) 1216
SlaI CTCGAG 1 cut(s) 554
SmiMI CAYNNNNRTG 1 cut(s) 641
SmlI CTYRAG 3 cut(s) 554, 1149, 1163
SmoI CTYRAG 3 cut(s) 554, 1149, 1163
SphI GCATGC 1 cut(s) 1575
SsiI CCGC 3 cut(s) 1260, 1575, 1619
SspDI GGCGCC 1 cut(s) 1238
SspI AATATT 1 cut(s) 878
SspMI CTAG 7 cut(s) 239, 533, 888, 968, 1145, 1250, 1310
SstI GAGCTC 1 cut(s) 975
StyD4I CCNGG 2 cut(s) 915, 1322
StyI CCWWGG 1 cut(s) 238
TaaI ACNGT 2 cut(s) 377, 509
TaiI ACGT 3 cut(s) 40, 795, 1065
TatI WGTACW 1 cut(s) 408
TauI GCSGC 1 cut(s) 1578
TfiI GAWTC 3 cut(s) 443, 1113, 1295
Tru1I TTAA 6 cut(s) 156, 438, 473, 575, 611, 1362
Tru9I TTAA 6 cut(s) 156, 438, 473, 575, 611, 1362
TscAI CASTG 3 cut(s) 1026, 1251, 1551
TseI GCWGC 3 cut(s) 736, 739, 805
TspRI CASTG 3 cut(s) 1026, 1251, 1551
VpaK11BI GGWCC 1 cut(s) 1216
XagI CCTNNNNNAGG 1 cut(s) 886
XapI RAATTY 5 cut(s) 276, 288, 434, 1436, 1557
XceI RCATGY 2 cut(s) 1406, 1575
XcmI CCANNNNNNNNNTGG 1 cut(s) 302
XhoI CTCGAG 1 cut(s) 554
XmaJI CCTAGG 1 cut(s) 238
XmiI GTMKAC 2 cut(s) 1067, 1477
XspI CTAG 7 cut(s) 239, 533, 888, 968, 1145, 1250, 1310
ZrmI AGTACT 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.