Rroxscaffold_2G00123030

(-)-germacrene D synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000053
Physical Location & Seq
Reverse (-)
56629664 .. 56633210
3547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00123030.1

Sequence Viewer

Length: 1416 bp
ATGTCTCTCCAAGTTTTGGCATCTCAAGCTCAAAGGCCAAATACCATTGCTGATGTTAAGCGACCTTCAGCTAATTTCCCCCTAAGCATTTGGGGTGATCATTTTCTCTCTTATGTTACCACGTTCCATATAGTTGACATTAAACTTGAGCAACACGTTCGAAAACTAAAAGAAGAGGTGAAGAGGATGCTAAAGGATCCCTTAAACAAAACTTCACAACAATTGGACTTGATTGATGACATTCAACGCCTAGGCGTGTCGTATCATTTTGAGAATGAGATTGATGAAATTTTGAAACAAATTCACCACAACTACTCTTATGGTAGTGAAGATGACCTTTACACTACTGCTCTTCGTTTTCGATTGCTGAGGCAACAAGGTTATAATGTTTCATGTGGCACTCATCTTAGGATTCATGGAGAAGACATACTCGATGAGGCACTAGCCTTCACCGCTACTCATCTTGAGTCGATCAAACACAGCTTGAGCCCTTCACTTTCAAAACGAGTAGCCCACTCCTTAATTCAGCCACTTCGAAAGGGCATCAACGAGTGGAAGCTAGCAAATTCATCAAAAGGAACTATATGGTGGAAGGACTTGGACGTGAGAAACACCATGCCATTTATAAGAGACAGAATTGTTGAAGCTTATTTCTGCTGGGTTTTGCCGGTGTATTTCGAACCTCGGTATTCCTTTGCTAGGAGGATAGCAAGCAAAGTCATCGCCATGACATCTATCATCGATGACATATATGATGCCCATGGCACGTTCGAAGAACTAGAGCTCTTTACTGAAGTTATTCAGAGGTGGGATATCTCTGCCATAAATCTATTGCCAGATTATATGAAAGTTTGTTATAAGGCATTGTTAGAAGTCTATACTGAAATTGAAGAATGGCTTGAAAATGGGGGAAAATTGTATCGAATCCACTACGCCATAGATGCTATGAAAAGGCAAGCTAAATATTACTTCCTTGAAGCTAAATGGCTCCACCAGAAATACATACCAACAATGGATGAATATATGGCCCTATCATTACTGTCCTCGGGCTACCCACTGCTAATACTCACCTCTTTTGTTGGAATGGGAGATATTGCTACACAAGACTCCTTTGATTGGCTAACGACTTTTCCCAGGGTTGTGAAAGGGGCTGCAATAGTTGCCCGACTTATGAATGACATAGTGTCCCACAAGGTACCTGTGGAGTGTTACATGAAAGAATTTAGTGCCACAGAAGAAGAAGCAATAATCGGATTGCATAGACAAGTGAGTGATGCATGGAAGGACATAAATGAATCGTTCCTCCTCCCTAATGCTGTTTCAATGCCACTGCAAACTCGAATTCTCAATTATGCATGTGCTGCAGATGTTGTTTACAAGTATGGAGATGGTTACACTTGTGGATATACTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

471

Amino Acids

54.53

Weight (kDa)

5.99

Isoelectric Point (pI)

45.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 30 - 132 9.6e-32 Terpene synthase, N-terminal domain
Terpene_synth PF01397 134 - 174 4.6e-07 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 198 - 429 1.6e-83 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 239 - 429 1.1e-49 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 384, 626, 858
Acc65I GGTACC 1 cut(s) 1195
AccB1I GGYRCC 1 cut(s) 1195
AccB7I CCANNNNNTGG 1 cut(s) 16
AciI CCGC 1 cut(s) 453
AclWI GGATC 2 cut(s) 191, 204
AcsI RAATTY 5 cut(s) 288, 300, 565, 1220, 1341
AcuI CTGAAG 2 cut(s) 51, 813
AfaI GTAC 1 cut(s) 1197
AfiI CCNNNNNNNGG 3 cut(s) 16, 699, 1116
AflIII ACRYGT 1 cut(s) 154
AgsI TTSAA 8 cut(s) 245, 295, 501, 644, 890, 902, 977, 1323
AhdI GACNNNNNGTC 1 cut(s) 1183
AjiI CACGTC 1 cut(s) 604
AjnI CCWGG 1 cut(s) 1133
AluBI AGCT 8 cut(s) 29, 71, 483, 559, 647, 784, 959, 980
AluI AGCT 8 cut(s) 29, 71, 483, 559, 647, 784, 959, 980
Alw21I GWGCWC 1 cut(s) 786
Alw26I GTCTC 2 cut(s) 9, 624
AlwI GGATC 2 cut(s) 191, 204
Ama87I CYCGRG 1 cut(s) 1045
AoxI GGCC 2 cut(s) 35, 1026
ApeKI GCWGC 2 cut(s) 1151, 1361
ApoI RAATTY 5 cut(s) 288, 300, 565, 1220, 1341
Asp700I GAANNNNTTC 1 cut(s) 798
Asp718I GGTACC 1 cut(s) 1195
AspA2I CCTAGG 1 cut(s) 250
AspS9I GGNCC 1 cut(s) 1027
AsuHPI GGTGA 5 cut(s) 107, 190, 296, 442, 1060
AsuII TTCGAA 4 cut(s) 160, 535, 678, 771
AsuNHI GCTAGC 1 cut(s) 559
AvaI CYCGRG 1 cut(s) 1045
AvrII CCTAGG 1 cut(s) 250
BamHI GGATCC 1 cut(s) 196
BanI GGYRCC 1 cut(s) 1195
BanII GRGCYC 2 cut(s) 491, 786
BbsI GAAGAC 1 cut(s) 429
Bbv12I GWGCWC 1 cut(s) 786
BbvCI CCTCAGC 1 cut(s) 368
BbvI GCAGC 2 cut(s) 1138, 1348
BccI CCATC 1 cut(s) 1382
BcgI CGANNNNNNTGC 4 cut(s) 140, 174, 703, 737
BciT130I CCWGG 1 cut(s) 1135
BclI TGATCA 1 cut(s) 97
BcoDI GTCTC 2 cut(s) 9, 624
BfaI CTAG 5 cut(s) 251, 443, 560, 699, 779
BfmI CTRYAG 1 cut(s) 1362
BisI GCNGC 2 cut(s) 1152, 1362
BlnI CCTAGG 1 cut(s) 250
BlsI GCNGC 2 cut(s) 1153, 1363
Bme1390I CCNGG 1 cut(s) 1135
BmeRI GACNNNNNGTC 1 cut(s) 1183
BmeT110I CYCGRG 1 cut(s) 1045
BmgBI CACGTC 1 cut(s) 604
BmgT120I GGNCC 1 cut(s) 1027
BmiI GGNNCC 3 cut(s) 198, 989, 1197
BmrFI CCNGG 1 cut(s) 1135
BmsI GCATC 6 cut(s) 29, 177, 552, 745, 931, 1264
BmtI GCTAGC 1 cut(s) 563
BpiI GAAGAC 1 cut(s) 429
Bpu10I CCTNAGC 2 cut(s) 83, 368
Bpu14I TTCGAA 4 cut(s) 160, 535, 678, 771
BpuEI CTTGAG 4 cut(s) 9, 167, 485, 505
Bsa29I ATCGAT 1 cut(s) 741
BsaJI CCNNGG 6 cut(s) 250, 683, 760, 1044, 1133, 1134
Bsc4I CCNNNNNNNGG 3 cut(s) 16, 699, 1116
Bse118I RCCGGY 1 cut(s) 667
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 1 cut(s) 1135
BseCI ATCGAT 1 cut(s) 741
BseDI CCNNGG 6 cut(s) 250, 683, 760, 1044, 1133, 1134
BseGI GGATG 2 cut(s) 192, 1021
BseLI CCNNNNNNNGG 3 cut(s) 16, 699, 1116
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 1 cut(s) 359
BseRI GAGGAG 1 cut(s) 1295
BseXI GCAGC 2 cut(s) 1138, 1348
BseYI CCCAGC 1 cut(s) 657
BshFI GGCC 2 cut(s) 37, 1028
BshNI GGYRCC 1 cut(s) 1195
BshVI ATCGAT 1 cut(s) 741
BsiHKAI GWGCWC 1 cut(s) 786
BsiHKCI CYCGRG 1 cut(s) 1045
BsiSI CCGG 1 cut(s) 668
BslFI GGGAC 1 cut(s) 1171
BslI CCNNNNNNNGG 3 cut(s) 16, 699, 1116
BsmAI GTCTC 2 cut(s) 9, 624
BsmFI GGGAC 1 cut(s) 1171
BsnI GGCC 2 cut(s) 37, 1028
BsoBI CYCGRG 1 cut(s) 1045
Bsp119I TTCGAA 4 cut(s) 160, 535, 678, 771
Bsp1286I GDGCHC 2 cut(s) 491, 786
Bsp143I GATC 3 cut(s) 97, 196, 471
Bsp19I CCATGG 1 cut(s) 760
BspACI CCGC 1 cut(s) 453
BspANI GGCC 2 cut(s) 37, 1028
BspCNI CTCAG 1 cut(s) 360
BspDI ATCGAT 1 cut(s) 741
BspLI GGNNCC 3 cut(s) 198, 989, 1197
BspMAI CTGCAG 1 cut(s) 1366
BspOI GCTAGC 1 cut(s) 563
BspPI GGATC 2 cut(s) 191, 204
BspQI GCTCTTC 1 cut(s) 357
BspT104I TTCGAA 4 cut(s) 160, 535, 678, 771
BspT107I GGYRCC 1 cut(s) 1195
BsrDI GCAATG 1 cut(s) 45
BsrFI RCCGGY 1 cut(s) 667
BssAI RCCGGY 1 cut(s) 667
BssECI CCNNGG 6 cut(s) 250, 683, 760, 1044, 1133, 1134
BssMI GATC 3 cut(s) 97, 196, 471
BssT1I CCWWGG 2 cut(s) 250, 760
Bst2UI CCWGG 1 cut(s) 1135
Bst4CI ACNGT 1 cut(s) 1041
Bst6I CTCTTC 3 cut(s) 168, 176, 357
BstAPI GCANNNNNTGC 2 cut(s) 1160, 1361
BstBI TTCGAA 4 cut(s) 160, 535, 678, 771
BstC8I GCNNGC 3 cut(s) 561, 712, 957
BstDEI CTNAG 3 cut(s) 83, 368, 407
BstDSI CCRYGG 1 cut(s) 760
BstENI CCTNNNNNAGG 1 cut(s) 697
BstF5I GGATG 2 cut(s) 192, 1021
BstKTI GATC 3 cut(s) 100, 199, 474
BstMAI GTCTC 2 cut(s) 9, 624
BstMBI GATC 3 cut(s) 97, 196, 471
BstMWI GCNNNNNNNGC 5 cut(s) 26, 452, 941, 1160, 1361
BstNI CCWGG 1 cut(s) 1135
BstNSI RCATGY 1 cut(s) 1359
BstSCI CCNGG 1 cut(s) 1133
BstSFI CTRYAG 1 cut(s) 1362
BstV1I GCAGC 2 cut(s) 1138, 1348
BstV2I GAAGAC 1 cut(s) 429
BstX2I RGATCY 1 cut(s) 196
BstYI RGATCY 1 cut(s) 196
Bsu15I ATCGAT 1 cut(s) 741
BsuRI GGCC 2 cut(s) 37, 1028
BsuTUI ATCGAT 1 cut(s) 741
BtgI CCRYGG 1 cut(s) 760
BtgZI GCGATG 1 cut(s) 706
BtrI CACGTC 1 cut(s) 604
BtsCI GGATG 2 cut(s) 192, 1021
BtsI GCAGTG 2 cut(s) 1055, 1328
BtsIMutI CAGTG 2 cut(s) 1055, 1328
Cac8I GCNNGC 3 cut(s) 561, 712, 957
Cfr10I RCCGGY 1 cut(s) 667
Cfr13I GGNCC 1 cut(s) 1027
ClaI ATCGAT 1 cut(s) 741
Csp6I GTAC 1 cut(s) 1196
CviAII CATG 8 cut(s) 393, 416, 616, 727, 761, 1213, 1278, 1356
CviQI GTAC 1 cut(s) 1196
DdeI CTNAG 3 cut(s) 83, 368, 407
DpnI GATC 3 cut(s) 99, 198, 473
DpnII GATC 3 cut(s) 97, 196, 471
DriI GACNNNNNGTC 1 cut(s) 1183
Eam1104I CTCTTC 3 cut(s) 168, 176, 357
Eam1105I GACNNNNNGTC 1 cut(s) 1183
EarI CTCTTC 3 cut(s) 168, 176, 357
Ecl136II GAGCTC 1 cut(s) 784
Eco130I CCWWGG 2 cut(s) 250, 760
Eco24I GRGCYC 2 cut(s) 491, 786
Eco32I GATATC 1 cut(s) 814
Eco53kI GAGCTC 1 cut(s) 784
Eco57I CTGAAG 2 cut(s) 51, 813
Eco88I CYCGRG 1 cut(s) 1045
EcoICRI GAGCTC 1 cut(s) 784
EcoNI CCTNNNNNAGG 1 cut(s) 697
EcoRI GAATTC 1 cut(s) 1341
EcoRII CCWGG 1 cut(s) 1133
EcoRV GATATC 1 cut(s) 814
EcoT14I CCWWGG 2 cut(s) 250, 760
EcoT22I ATGCAT 2 cut(s) 1279, 1357
EcoT38I GRGCYC 2 cut(s) 491, 786
ErhI CCWWGG 2 cut(s) 250, 760
FaeI CATG 8 cut(s) 396, 419, 619, 730, 764, 1216, 1281, 1359
FalI AAGNNNNNCTT 6 cut(s) 185, 217, 321, 353, 882, 914
FaqI GGGAC 1 cut(s) 1171
FatI CATG 8 cut(s) 392, 415, 615, 726, 760, 1212, 1277, 1355
FbaI TGATCA 1 cut(s) 97
Fnu4HI GCNGC 2 cut(s) 1152, 1362
FokI GGATG 2 cut(s) 199, 1028
FriOI GRGCYC 2 cut(s) 491, 786
Fsp4HI GCNGC 2 cut(s) 1152, 1362
FspBI CTAG 5 cut(s) 251, 443, 560, 699, 779
GluI GCNGC 2 cut(s) 1152, 1362
GsaI CCCAGC 1 cut(s) 661
HaeIII GGCC 2 cut(s) 37, 1028
HapII CCGG 1 cut(s) 668
Hin1II CATG 8 cut(s) 396, 419, 619, 730, 764, 1216, 1281, 1359
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HindIII AAGCTT 1 cut(s) 645
HinfI GANTC 5 cut(s) 412, 467, 924, 1106, 1295
HpaII CCGG 1 cut(s) 668
HphI GGTGA 5 cut(s) 107, 190, 296, 442, 1060
Hpy166II GTNNAC 2 cut(s) 136, 1375
Hpy188I TCNGA 2 cut(s) 804, 1253
Hpy188III TCNNGA 1 cut(s) 464
Hpy8I GTNNAC 2 cut(s) 136, 1375
HpyAV CCTTC 5 cut(s) 75, 457, 501, 586, 1276
HpyCH4III ACNGT 1 cut(s) 1041
HpyCH4IV ACGT 4 cut(s) 122, 156, 603, 767
HpyCH4V TGCA 6 cut(s) 1154, 1258, 1277, 1333, 1355, 1364
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 452, 941, 1160, 1361
HpyF3I CTNAG 3 cut(s) 83, 368, 407
HpySE526I ACGT 4 cut(s) 122, 156, 603, 767
Hsp92II CATG 8 cut(s) 396, 419, 619, 730, 764, 1216, 1281, 1359
KpnI GGTACC 1 cut(s) 1199
Ksp22I TGATCA 1 cut(s) 97
Kzo9I GATC 3 cut(s) 97, 196, 471
LguI GCTCTTC 1 cut(s) 357
LmnI GCTCC 1 cut(s) 993
LpnPI CCDG 7 cut(s) 643, 681, 849, 1007, 1120, 1147, 1212
Lsp1109I GCAGC 2 cut(s) 1138, 1348
LweI GCATC 6 cut(s) 29, 177, 552, 745, 931, 1264
MaeI CTAG 5 cut(s) 251, 443, 560, 699, 779
MaeII ACGT 4 cut(s) 122, 156, 603, 767
MaeIII GTNAC 3 cut(s) 115, 1208, 1391
MalI GATC 3 cut(s) 99, 198, 473
MboI GATC 3 cut(s) 97, 196, 471
MboII GAAGA 9 cut(s) 185, 193, 341, 344, 434, 785, 902, 1247, 1250
MfeI CAATTG 1 cut(s) 221
MflI RGATCY 1 cut(s) 196
MhlI GDGCHC 2 cut(s) 491, 786
MlyI GAGTC 2 cut(s) 476, 1100
MmeI TCCRAC 1 cut(s) 1060
Mph1103I ATGCAT 2 cut(s) 1279, 1357
MroXI GAANNNNTTC 1 cut(s) 798
MseI TTAA 4 cut(s) 57, 141, 203, 521
MslI CAYNNNNRTG 1 cut(s) 725
MspI CCGG 1 cut(s) 668
MspR9I CCNGG 1 cut(s) 1135
MunI CAATTG 1 cut(s) 221
MvaI CCWGG 1 cut(s) 1135
MwoI GCNNNNNNNGC 5 cut(s) 26, 452, 941, 1160, 1361
NcoI CCATGG 1 cut(s) 760
NdeII GATC 3 cut(s) 97, 196, 471
NheI GCTAGC 1 cut(s) 559
NlaIII CATG 8 cut(s) 396, 419, 619, 730, 764, 1216, 1281, 1359
NlaIV GGNNCC 3 cut(s) 198, 989, 1197
NsiI ATGCAT 2 cut(s) 1279, 1357
NspI RCATGY 1 cut(s) 1359
NspV TTCGAA 4 cut(s) 160, 535, 678, 771
PasI CCCWGGG 1 cut(s) 1134
PciSI GCTCTTC 1 cut(s) 357
PdmI GAANNNNTTC 1 cut(s) 798
PfeI GAWTC 3 cut(s) 412, 924, 1295
PflMI CCANNNNNTGG 1 cut(s) 16
PkrI GCNGC 2 cut(s) 1153, 1363
PleI GAGTC 2 cut(s) 475, 1100
PpsI GAGTC 2 cut(s) 475, 1100
PsiI TTATAA 3 cut(s) 384, 626, 858
Psp124BI GAGCTC 1 cut(s) 786
Psp6I CCWGG 1 cut(s) 1133
PspFI CCCAGC 1 cut(s) 657
PspGI CCWGG 1 cut(s) 1133
PspN4I GGNNCC 3 cut(s) 198, 989, 1197
PspPI GGNCC 1 cut(s) 1027
PstI CTGCAG 1 cut(s) 1366
PsuI RGATCY 1 cut(s) 196
RsaI GTAC 1 cut(s) 1197
RsaNI GTAC 1 cut(s) 1196
RseI CAYNNNNRTG 1 cut(s) 725
SacI GAGCTC 1 cut(s) 786
SapI GCTCTTC 1 cut(s) 357
SaqAI TTAA 4 cut(s) 57, 141, 203, 521
SatI GCNGC 2 cut(s) 1152, 1362
Sau3AI GATC 3 cut(s) 97, 196, 471
Sau96I GGNCC 1 cut(s) 1027
SchI GAGTC 2 cut(s) 476, 1100
ScrFI CCNGG 1 cut(s) 1135
SduI GDGCHC 2 cut(s) 491, 786
SfaNI GCATC 6 cut(s) 29, 177, 552, 745, 931, 1264
SfcI CTRYAG 1 cut(s) 1362
SfuI TTCGAA 4 cut(s) 160, 535, 678, 771
SmiMI CAYNNNNRTG 1 cut(s) 725
SmlI CTYRAG 4 cut(s) 24, 146, 464, 484
SmoI CTYRAG 4 cut(s) 24, 146, 464, 484
SsiI CCGC 1 cut(s) 453
SspI AATATT 1 cut(s) 965
SspMI CTAG 5 cut(s) 251, 443, 560, 699, 779
SstI GAGCTC 1 cut(s) 786
StyD4I CCNGG 1 cut(s) 1133
StyI CCWWGG 2 cut(s) 250, 760
TaaI ACNGT 1 cut(s) 1041
TaiI ACGT 4 cut(s) 125, 159, 606, 770
TfiI GAWTC 3 cut(s) 412, 924, 1295
Tru1I TTAA 4 cut(s) 57, 141, 203, 521
Tru9I TTAA 4 cut(s) 57, 141, 203, 521
TscAI CASTG 2 cut(s) 1062, 1335
TseI GCWGC 2 cut(s) 1151, 1361
TspRI CASTG 2 cut(s) 1062, 1335
Van91I CCANNNNNTGG 1 cut(s) 16
XagI CCTNNNNNAGG 1 cut(s) 697
XapI RAATTY 5 cut(s) 288, 300, 565, 1220, 1341
XceI RCATGY 1 cut(s) 1359
XmaJI CCTAGG 1 cut(s) 250
XmnI GAANNNNTTC 1 cut(s) 798
XspI CTAG 5 cut(s) 251, 443, 560, 699, 779
Zsp2I ATGCAT 2 cut(s) 1279, 1357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.