Rw1G005940

synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000053
Physical Location & Seq
Forward (+)
12035590 .. 12038814
3225 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G005940.1

Sequence Viewer

Length: 1704 bp
ATGGATCAAGCACGAACAGCAAATGCCAGTAGCACTCTTGATGTTGATCGACGTTCAGCTAATTTTTCTCCCAGCATTTGGGGCGAGTATTTTCTGTCATATGCATCAATGGAAACTGCAGATATTGAATCCAACCAAAGTATCCAAGAACTGAAGGAAGAAGTGAAGAGGATGCTAATGAGTTCTCTGTCTTTACAAAGATTACACTTGATTGATTACATTCAGCGCTTGGGGTTGTCCTACCATTTTGAGGATGAGATTTACCAACTTTTACATCAAATTTACAACCGGTCCTGTTCTTCTTATGATGATGATGATGATGATGATGATGACGACGACGACGACCTTCACACAGTTGCTCTCCGATTTCGGCTGCTTAGGCAACAAGGTTTTAAGGTTTCATGCAACATGTTCAACAAGTTCATCGATGTTGATGGGAAATTTAAGGAATCATGCGTTGCTGATGTAGTAGGATTGCTAAGTTTGTACGAAGCAACACATCTTAGGACACATGGAGATGATATATTAGATCGAGCACGGTCCTTCACCACCACTCATCTTGAGTTTGCAGTGGCAGAACACGGCCGTTTAAGCCCCCCGCTTTCAAAACAAGTAGCTCATGCCTTATATCAACCACTCTGGAAGGGAAACCCAAGGATTGAAGCGAGGCATTACCTCTCTAGCTACCGGGAACTAAATACTTCACATTTTAGCGAAAGTCTTTTGACTTTTGCGAAGTTGGATTTCAATCAATTGCAGCGAATCCATCAAAAAGAACTAAGTCACATCACAAGGTGGTGGAAGGAGTTGGACTTTGTCAACAAACTACCTTTTGCAAGGGACAGAAAAGTAGAGTGTTACTTTTGGTCTTTGGGAGTCTACTTTGAGCCCGAGTATCATTTTGGGAGGACAACATTATGCAAAGTTCTTGGCTTACTAACCATGATTGATGACACTTACGATACGTACGGCACACCTGAAGAACTAGAGCTCTTTACGGAAGCTATTGAGAGGTGGGATATCTCTGCCATGGATTCATTGCCTGACTATATGAAAGTTTGCTATGAGGCATTGTTGAATGTCTACACTGAAATTAAAGATGAGCTTGCAAAGGAGGGAAACTCGTATCGCATCAACTATGCAATAGAAGCAGTGCAAATTCAAGTTAGATCTTACTTAGAGGAAGCCAAATGGTTCCACCAAAAGTACACACCGAAAACCTTTGATGAGTATATGTCGGTGGCACTTGTATCATCCGGCATGTTTATGGTGGAAGCAACATTTATCGTTTCAATAGCTGGTGATATTGTTACAAGAGATTCCTTGGACTGGTTGTTCAGTGACCCTAAATTTGTAAAGGCTTCATCGATAATTGGGAGACTCTTGAATGACATCCGAAGCCACAAGCTTGAGCAAAAGAGAGGACATGTTGTCTCAGCTGTGGAATGCTATATGAAAGAACACTGTGCTACAGAAGAAGTAGCGGTGATTGAACTGACTAAACATGTAAATGATGCATGGACGGATGTAAACGAAGGATTGCTTCACCCTATCGCTACTGTCCCTAGGCCACTACTATTGCTAATTCTCAATTTTCTACGCGTAACTGATGTTATATACAAGCATGAAGATGGTTATACTCATGGTGGAGTTGTGCTTAAGGATTATATAACTTCTCTTCTTGTCGAACATGTTCCAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

567

Amino Acids

65.76

Weight (kDa)

5.17

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 26 - 209 2.2e-58 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 268 - 509 1.4e-95 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 308 - 509 2.2e-54 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 78
AccI GTMKAC 2 cut(s) 879, 1083
AccII CGCG 1 cut(s) 1602
AciI CCGC 2 cut(s) 599, 1484
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 583
AcsI RAATTY 4 cut(s) 279, 440, 1158, 1349
AcuI CTGAAG 2 cut(s) 173, 999
AdeI CACNNNGTG 1 cut(s) 795
AfaI GTAC 3 cut(s) 488, 968, 1208
AfeI AGCGCT 1 cut(s) 227
AfiI CCNNNNNNNGG 3 cut(s) 78, 250, 1329
AflII CTTAAG 1 cut(s) 1658
AflIII ACRYGT 5 cut(s) 408, 1426, 1504, 1600, 1690
AgeI ACCGGT 1 cut(s) 288
AhdI GACNNNNNGTC 1 cut(s) 1430
AloI GAACNNNNNNTCC 2 cut(s) 1319, 1351
AluBI AGCT 9 cut(s) 59, 617, 684, 991, 1004, 1105, 1298, 1408, 1439
AluI AGCT 9 cut(s) 59, 617, 684, 991, 1004, 1105, 1298, 1408, 1439
Alw21I GWGCWC 2 cut(s) 538, 993
Alw26I GTCTC 2 cut(s) 1372, 1438
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 1 cut(s) 890
Aor51HI AGCGCT 1 cut(s) 227
AoxI GGCC 2 cut(s) 583, 1568
ApeKI GCWGC 2 cut(s) 373, 757
ApoI RAATTY 4 cut(s) 279, 440, 1158, 1349
ArsI GACNNNNNNTTYG 2 cut(s) 1334, 1366
AsiGI ACCGGT 1 cut(s) 288
Asp700I GAANNNNTTC 1 cut(s) 1692
AspA2I CCTAGG 1 cut(s) 1565
AspLEI GCGC 1 cut(s) 228
AspS9I GGNCC 2 cut(s) 291, 540
AsuC2I CCSGG 1 cut(s) 689
AsuHPI GGTGA 4 cut(s) 538, 1313, 1498, 1538
AvaI CYCGRG 1 cut(s) 890
AvaII GGWCC 2 cut(s) 291, 540
AvrII CCTAGG 1 cut(s) 1565
BaeI ACNNNNGTAYC 2 cut(s) 124, 157
BanII GRGCYC 2 cut(s) 891, 993
Bbv12I GWGCWC 2 cut(s) 538, 993
BbvI GCAGC 2 cut(s) 360, 769
BccI CCATC 3 cut(s) 428, 774, 1625
BceAI ACGGC 3 cut(s) 570, 598, 985
BciVI GTATCC 1 cut(s) 152
BcnI CCSGG 1 cut(s) 689
BcoDI GTCTC 2 cut(s) 1372, 1438
BfaI CTAG 3 cut(s) 681, 986, 1566
BfmI CTRYAG 2 cut(s) 117, 1470
BfoI RGCGCY 1 cut(s) 229
BfrI CTTAAG 1 cut(s) 1658
BfuI GTATCC 1 cut(s) 152
BglII AGATCT 1 cut(s) 1169
BisI GCNGC 2 cut(s) 374, 758
BlnI CCTAGG 1 cut(s) 1565
BlsI GCNGC 2 cut(s) 375, 759
Bme1390I CCNGG 1 cut(s) 689
Bme18I GGWCC 2 cut(s) 291, 540
BmeRI GACNNNNNGTC 1 cut(s) 1430
BmeT110I CYCGRG 1 cut(s) 890
BmgT120I GGNCC 2 cut(s) 291, 540
BmiI GGNNCC 1 cut(s) 1196
BmrFI CCNGG 1 cut(s) 689
BmsI GCATC 4 cut(s) 113, 162, 1140, 1504
BplI GAGNNNNNCTC 2 cut(s) 1106, 1138
Bpu10I CCTNAGC 1 cut(s) 377
BpuEI CTTGAG 2 cut(s) 581, 1430
BpuMI CCSGG 1 cut(s) 689
Bsa29I ATCGAT 2 cut(s) 426, 1367
BsaAI YACGTR 1 cut(s) 966
BsaBI GATNNNNATC 2 cut(s) 45, 747
BsaJI CCNNGG 4 cut(s) 653, 1029, 1323, 1565
BsaWI WCCGGW 1 cut(s) 288
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 250, 1329
Bse118I RCCGGY 1 cut(s) 288
Bse1I ACTGG 2 cut(s) 27, 1334
Bse3DI GCAATG 1 cut(s) 1037
Bse8I GATNNNNATC 2 cut(s) 45, 747
BseCI ATCGAT 2 cut(s) 426, 1367
BseDI CCNNGG 4 cut(s) 653, 1029, 1323, 1565
BseGI GGATG 5 cut(s) 177, 259, 1253, 1392, 1531
BseJI GATNNNNATC 2 cut(s) 45, 747
BseLI CCNNNNNNNGG 3 cut(s) 78, 250, 1329
BseMI GCAATG 1 cut(s) 1037
BseMII CTCAG 1 cut(s) 1449
BseNI ACTGG 2 cut(s) 27, 1334
BseX3I CGGCCG 1 cut(s) 583
BseXI GCAGC 2 cut(s) 360, 769
BseYI CCCAGC 1 cut(s) 71
Bsh1236I CGCG 1 cut(s) 1602
Bsh1285I CGRYCG 1 cut(s) 586
BshFI GGCC 2 cut(s) 585, 1570
BshTI ACCGGT 1 cut(s) 288
BshVI ATCGAT 2 cut(s) 426, 1367
BsiEI CGRYCG 1 cut(s) 586
BsiHKAI GWGCWC 2 cut(s) 538, 993
BsiHKCI CYCGRG 1 cut(s) 890
BsiSI CCGG 3 cut(s) 289, 688, 1257
BsiWI CGTACG 1 cut(s) 966
BslFI GGGAC 2 cut(s) 854, 1547
BslI CCNNNNNNNGG 3 cut(s) 78, 250, 1329
BsmAI GTCTC 2 cut(s) 1372, 1438
BsmFI GGGAC 2 cut(s) 854, 1547
BsmI GAATGC 1 cut(s) 1451
BsnI GGCC 2 cut(s) 585, 1570
BsoBI CYCGRG 1 cut(s) 890
Bsp1286I GDGCHC 3 cut(s) 538, 891, 993
Bsp143I GATC 4 cut(s) 4, 46, 529, 1169
Bsp19I CCATGG 1 cut(s) 1029
BspACI CCGC 2 cut(s) 599, 1484
BspANI GGCC 2 cut(s) 585, 1570
BspCNI CTCAG 1 cut(s) 1448
BspDI ATCGAT 2 cut(s) 426, 1367
BspFNI CGCG 1 cut(s) 1602
BspLI GGNNCC 1 cut(s) 1196
BspMAI CTGCAG 1 cut(s) 121
BspPI GGATC 1 cut(s) 12
BspTI CTTAAG 1 cut(s) 1658
BsrDI GCAATG 1 cut(s) 1037
BsrFI RCCGGY 1 cut(s) 288
BsrI ACTGG 2 cut(s) 27, 1334
BssAI RCCGGY 1 cut(s) 288
BssECI CCNNGG 4 cut(s) 653, 1029, 1323, 1565
BssMI GATC 4 cut(s) 4, 46, 529, 1169
BssT1I CCWWGG 4 cut(s) 653, 1029, 1323, 1565
Bst4CI ACNGT 4 cut(s) 355, 540, 1466, 1561
Bst6I CTCTTC 2 cut(s) 161, 1683
BstAFI CTTAAG 1 cut(s) 1658
BstBAI YACGTR 1 cut(s) 966
BstC8I GCNNGC 1 cut(s) 1107
BstDEI CTNAG 6 cut(s) 377, 479, 503, 779, 1177, 1435
BstDSI CCRYGG 1 cut(s) 1029
BstF5I GGATG 5 cut(s) 177, 259, 1253, 1392, 1531
BstFNI CGCG 1 cut(s) 1602
BstH2I RGCGCY 1 cut(s) 229
BstHHI GCGC 1 cut(s) 228
BstKTI GATC 4 cut(s) 7, 49, 532, 1172
BstMAI GTCTC 2 cut(s) 1372, 1438
BstMBI GATC 4 cut(s) 4, 46, 529, 1169
BstMCI CGRYCG 1 cut(s) 586
BstMWI GCNNNNNNNGC 5 cut(s) 17, 81, 379, 591, 1148
BstNSI RCATGY 5 cut(s) 412, 1264, 1430, 1508, 1694
BstSCI CCNGG 1 cut(s) 687
BstSFI CTRYAG 2 cut(s) 117, 1470
BstSNI TACGTA 1 cut(s) 966
BstUI CGCG 1 cut(s) 1602
BstV1I GCAGC 2 cut(s) 360, 769
BstX2I RGATCY 1 cut(s) 1169
BstYI RGATCY 1 cut(s) 1169
BstZI CGGCCG 1 cut(s) 583
Bsu15I ATCGAT 2 cut(s) 426, 1367
BsuI GTATCC 1 cut(s) 152
BsuRI GGCC 2 cut(s) 585, 1570
BsuTUI ATCGAT 2 cut(s) 426, 1367
BtgI CCRYGG 1 cut(s) 1029
BtsCI GGATG 5 cut(s) 177, 259, 1253, 1392, 1531
BtsI GCAGTG 2 cut(s) 576, 1158
BtsIMutI CAGTG 5 cut(s) 576, 1086, 1158, 1345, 1462
Cac8I GCNNGC 1 cut(s) 1107
CfoI GCGC 1 cut(s) 228
Cfr10I RCCGGY 1 cut(s) 288
Cfr13I GGNCC 2 cut(s) 291, 540
ClaI ATCGAT 2 cut(s) 426, 1367
Csp6I GTAC 3 cut(s) 487, 967, 1207
CspAI ACCGGT 1 cut(s) 288
CspCI CAANNNNNGTGG 4 cut(s) 541, 576, 1560, 1595
CviQI GTAC 3 cut(s) 487, 967, 1207
DdeI CTNAG 6 cut(s) 377, 479, 503, 779, 1177, 1435
DpnI GATC 4 cut(s) 6, 48, 531, 1171
DpnII GATC 4 cut(s) 4, 46, 529, 1169
DraIII CACNNNGTG 1 cut(s) 795
DriI GACNNNNNGTC 1 cut(s) 1430
EaeI YGGCCR 1 cut(s) 583
EagI CGGCCG 1 cut(s) 583
Eam1104I CTCTTC 2 cut(s) 161, 1683
Eam1105I GACNNNNNGTC 1 cut(s) 1430
EarI CTCTTC 2 cut(s) 161, 1683
Ecl136II GAGCTC 1 cut(s) 991
EclXI CGGCCG 1 cut(s) 583
Eco105I TACGTA 1 cut(s) 966
Eco130I CCWWGG 4 cut(s) 653, 1029, 1323, 1565
Eco24I GRGCYC 2 cut(s) 891, 993
Eco32I GATATC 1 cut(s) 1021
Eco47I GGWCC 2 cut(s) 291, 540
Eco47III AGCGCT 1 cut(s) 227
Eco52I CGGCCG 1 cut(s) 583
Eco53kI GAGCTC 1 cut(s) 991
Eco57I CTGAAG 2 cut(s) 173, 999
Eco88I CYCGRG 1 cut(s) 890
EcoICRI GAGCTC 1 cut(s) 991
EcoRV GATATC 1 cut(s) 1021
EcoT14I CCWWGG 4 cut(s) 653, 1029, 1323, 1565
EcoT22I ATGCAT 2 cut(s) 106, 1519
EcoT38I GRGCYC 2 cut(s) 891, 993
ErhI CCWWGG 4 cut(s) 653, 1029, 1323, 1565
FalI AAGNNNNNCTT 4 cut(s) 1089, 1121, 1527, 1559
FaqI GGGAC 2 cut(s) 854, 1547
FauI CCCGC 1 cut(s) 606
FauNDI CATATG 1 cut(s) 100
FblI GTMKAC 2 cut(s) 879, 1083
Fnu4HI GCNGC 2 cut(s) 374, 758
FokI GGATG 5 cut(s) 184, 266, 1240, 1379, 1538
FriOI GRGCYC 2 cut(s) 891, 993
Fsp4HI GCNGC 2 cut(s) 374, 758
FspBI CTAG 3 cut(s) 681, 986, 1566
GlaI GCGC 1 cut(s) 227
GluI GCNGC 2 cut(s) 374, 758
GsaI CCCAGC 1 cut(s) 75
HaeII RGCGCY 1 cut(s) 229
HaeIII GGCC 2 cut(s) 585, 1570
HapII CCGG 3 cut(s) 289, 688, 1257
HhaI GCGC 1 cut(s) 228
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HincII GTYRAC 1 cut(s) 820
HindII GTYRAC 1 cut(s) 820
HindIII AAGCTT 1 cut(s) 1406
HinfI GANTC 7 cut(s) 128, 449, 762, 876, 1034, 1319, 1380
HpaII CCGG 3 cut(s) 289, 688, 1257
HphI GGTGA 4 cut(s) 538, 1313, 1498, 1538
Hpy166II GTNNAC 5 cut(s) 820, 880, 1084, 1209, 1531
Hpy188I TCNGA 2 cut(s) 365, 1397
Hpy188III TCNNGA 4 cut(s) 38, 560, 640, 1384
Hpy8I GTNNAC 5 cut(s) 820, 880, 1084, 1209, 1531
Hpy99I CGWCG 4 cut(s) 54, 338, 341, 344
HpyAV CCTTC 6 cut(s) 148, 356, 553, 637, 796, 1529
HpyCH4III ACNGT 4 cut(s) 355, 540, 1466, 1561
HpyCH4IV ACGT 2 cut(s) 52, 965
HpyF10VI GCNNNNNNNGC 5 cut(s) 17, 81, 379, 591, 1148
HpyF3I CTNAG 6 cut(s) 377, 479, 503, 779, 1177, 1435
HpySE526I ACGT 2 cut(s) 52, 965
HspAI GCGC 1 cut(s) 226
Kzo9I GATC 4 cut(s) 4, 46, 529, 1169
Lsp1109I GCAGC 2 cut(s) 360, 769
LweI GCATC 4 cut(s) 113, 162, 1140, 1504
MaeI CTAG 3 cut(s) 681, 986, 1566
MaeII ACGT 2 cut(s) 52, 965
MaeIII GTNAC 5 cut(s) 782, 857, 1309, 1340, 1603
MalI GATC 4 cut(s) 6, 48, 531, 1171
MboI GATC 4 cut(s) 4, 46, 529, 1169
MboII GAAGA 7 cut(s) 170, 178, 291, 992, 1487, 1640, 1670
MfeI CAATTG 1 cut(s) 752
MflI RGATCY 1 cut(s) 1169
MhlI GDGCHC 3 cut(s) 538, 891, 993
MluI ACGCGT 1 cut(s) 1600
MlyI GAGTC 2 cut(s) 885, 1374
MmeI TCCRAC 3 cut(s) 156, 720, 789
Mph1103I ATGCAT 2 cut(s) 106, 1519
MroXI GAANNNNTTC 1 cut(s) 1692
MseI TTAA 5 cut(s) 393, 444, 590, 1095, 1659
MslI CAYNNNNRTG 4 cut(s) 516, 1265, 1509, 1629
MspA1I CMGCKG 1 cut(s) 1439
MspCI CTTAAG 1 cut(s) 1658
MspI CCGG 3 cut(s) 289, 688, 1257
MspR9I CCNGG 1 cut(s) 689
MunI CAATTG 1 cut(s) 752
Mva1269I GAATGC 1 cut(s) 1451
MvnI CGCG 1 cut(s) 1602
MwoI GCNNNNNNNGC 5 cut(s) 17, 81, 379, 591, 1148
NciI CCSGG 1 cut(s) 689
NcoI CCATGG 1 cut(s) 1029
NdeI CATATG 1 cut(s) 100
NdeII GATC 4 cut(s) 4, 46, 529, 1169
NlaIV GGNNCC 1 cut(s) 1196
NmuCI GTSAC 2 cut(s) 782, 1340
NsiI ATGCAT 2 cut(s) 106, 1519
NspI RCATGY 5 cut(s) 412, 1264, 1430, 1508, 1694
PciI ACATGT 4 cut(s) 408, 1426, 1504, 1690
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PctI GAATGC 1 cut(s) 1451
PdmI GAANNNNTTC 1 cut(s) 1692
PfeI GAWTC 5 cut(s) 128, 449, 762, 1034, 1319
Pfl23II CGTACG 1 cut(s) 966
PflFI GACNNNGTC 1 cut(s) 815
PflMI CCANNNNNTGG 1 cut(s) 78
PinAI ACCGGT 1 cut(s) 288
PkrI GCNGC 2 cut(s) 375, 759
PleI GAGTC 2 cut(s) 884, 1374
PpsI GAGTC 2 cut(s) 884, 1374
Ppu21I YACGTR 1 cut(s) 966
PscI ACATGT 4 cut(s) 408, 1426, 1504, 1690
Psp124BI GAGCTC 1 cut(s) 993
PspFI CCCAGC 1 cut(s) 71
PspLI CGTACG 1 cut(s) 966
PspN4I GGNNCC 1 cut(s) 1196
PspPI GGNCC 2 cut(s) 291, 540
PstI CTGCAG 1 cut(s) 121
PsuI RGATCY 1 cut(s) 1169
PsyI GACNNNGTC 1 cut(s) 815
PvuII CAGCTG 1 cut(s) 1439
RsaI GTAC 3 cut(s) 488, 968, 1208
RsaNI GTAC 3 cut(s) 487, 967, 1207
RseI CAYNNNNRTG 4 cut(s) 516, 1265, 1509, 1629
SacI GAGCTC 1 cut(s) 993
SaqAI TTAA 5 cut(s) 393, 444, 590, 1095, 1659
SatI GCNGC 2 cut(s) 374, 758
Sau3AI GATC 4 cut(s) 4, 46, 529, 1169
Sau96I GGNCC 2 cut(s) 291, 540
SchI GAGTC 2 cut(s) 885, 1374
ScrFI CCNGG 1 cut(s) 689
SduI GDGCHC 3 cut(s) 538, 891, 993
SfaNI GCATC 4 cut(s) 113, 162, 1140, 1504
SfcI CTRYAG 2 cut(s) 117, 1470
SinI GGWCC 2 cut(s) 291, 540
SmiMI CAYNNNNRTG 4 cut(s) 516, 1265, 1509, 1629
SmlI CTYRAG 3 cut(s) 560, 1409, 1658
SmoI CTYRAG 3 cut(s) 560, 1409, 1658
SnaBI TACGTA 1 cut(s) 966
SsiI CCGC 2 cut(s) 599, 1484
SspMI CTAG 3 cut(s) 681, 986, 1566
SstI GAGCTC 1 cut(s) 993
StyD4I CCNGG 1 cut(s) 687
StyI CCWWGG 4 cut(s) 653, 1029, 1323, 1565
TaaI ACNGT 4 cut(s) 355, 540, 1466, 1561
TaiI ACGT 2 cut(s) 55, 968
TaqI TCGA 5 cut(s) 49, 426, 532, 1367, 1686
TatI WGTACW 1 cut(s) 1206
TfiI GAWTC 5 cut(s) 128, 449, 762, 1034, 1319
Tru1I TTAA 5 cut(s) 393, 444, 590, 1095, 1659
Tru9I TTAA 5 cut(s) 393, 444, 590, 1095, 1659
TscAI CASTG 5 cut(s) 576, 1093, 1158, 1345, 1469
TseFI GTSAC 2 cut(s) 782, 1340
TseI GCWGC 2 cut(s) 373, 757
Tsp45I GTSAC 2 cut(s) 782, 1340
TspDTI ATGAA 7 cut(s) 390, 412, 1026, 1067, 1353, 1469, 1641
TspGWI ACGGA 2 cut(s) 1013, 1538
TspRI CASTG 5 cut(s) 576, 1093, 1158, 1345, 1469
Tth111I GACNNNGTC 1 cut(s) 815
Van91I CCANNNNNTGG 1 cut(s) 78
Vha464I CTTAAG 1 cut(s) 1658
VpaK11BI GGWCC 2 cut(s) 291, 540
XapI RAATTY 4 cut(s) 279, 440, 1158, 1349
XceI RCATGY 5 cut(s) 412, 1264, 1430, 1508, 1694
XmaJI CCTAGG 1 cut(s) 1565
XmiI GTMKAC 2 cut(s) 879, 1083
XmnI GAANNNNTTC 1 cut(s) 1692
XspI CTAG 3 cut(s) 681, 986, 1566
Zsp2I ATGCAT 2 cut(s) 106, 1519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.