Rroxscaffold_7G00197730

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000053
Physical Location & Seq
Reverse (-)
42203228 .. 42206573
3346 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00197730.1

Sequence Viewer

Length: 1566 bp
ATGTCGTCTACACCAACTAGTTCTGTATCATTTCCGAAGGCTGATGCCCTTTGGCAGCAGCAAGTTGACAAATTGAAAACAGTAGTGAAGAGGGAACTCTTTACTAATGAAGGTGATCTTTCACATCGACTCAAGTTAATTGATGCAATCCAGCGACTTGGGGTGGCATATCATTTTGAACGCGAAATAGAAGAATCACTGCAACATATCCATGCGACATATCGTGATCATGATCTGTACCGTGATGATGGCCATCTTTACGATGTTGCCCTATTATTTCGGCTTCTAAGACAACATGGATATCAAATTTCATCGGATATATTCAACAAGTTCAAAGATTCAAATGGTAGCTTCAAAGAATGCTTAATTTCTGATGTCTCCGGTATGCTAAGCTTCTATGAAGCAACACATCTTAGGGTTAATGGGGAAGATATACTTGAAGAGGCTCTTCTGTTCACCACCACTCAACTCAAGTACGTGCTCAAAAGCAATGTAATAAGCTATCCACCAGTATTGAAGGAACAAATAACTGAAGTTTTGACGAGACCTCTTCGAAAAACTTTGGAGAGATTATGCGCCAAGCGTTATATTTCAATCTACCAAGATGACGCCTCACACAGTGAATCTTTGTTGAAACTTGCAAAGTTGGATTTCAATCTTGTTCAATCTTTACACCAAAAGGAGCTCTCGATATTACTAGGTGGAATTACTAGGTGGTGGAAAGAACTAGACTTTGAAAGGAAATTGCCTTTTGCAAGAGACAGGGTGGTGGAGGTGTTCTTTTGGATGGTGGGAGTATATTTTGAACCCCAGTACTCTATTGGCAGAATCATTATGACAAAAGTGGGTATCCTCCTAACTATTTTGGATGATATCTATGATGCATATGGTACATTTGAAGAACTCATGATCTTCACTCAAGCAATTGACAGGTGGAATGTCAAATGCATAGATGAACTCCCAGACTATTTGAAAATATTCTATCATGAACTTTTGAATCTTTTTGATGAAATTGACAAATTGATGTCAAAGGAAGGAAGACCATATCGAACTTCATATGCAATAGAAGCTATGAAAGTTCAGGCTCGATCATTTTTCAACGAGACCCAATGGTTGCACGAAGGACGCACTCCGAGCATGGAGGAATACATGAGTGTTGCTACAGTTTCTGTTACTTACACTTTTATAACAACCATTTCATTACTTGGCATGGGAGATATTGTAACGAAGGATGCATTTGAGTGGTTGTTGGATGACCCGAAAATTGTTAGAGCTTCCAATATCATTTTTAGGCTCATGGATGACATTGTTTCCACCAAGTTTGAGAAAGAGAGAGGGCATGCACCTTCTAGCATTGATTGCTATATGAAGCAATATGGTGTTTTAGAACAAGAGACGATTGACGTTTTTGAGAAGCAAATTGTGAAATCATGGAAGGATATAAATGAGGAGTTTCTTAAACCAACCGCTATGCCAATGCCGGTTCTTATGCGTGTTCTCAATTTAACAAGAGTAGTGGATCTTCTTTACAAGGAGAAGATGGATTCACACGAGTTGGGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

521

Amino Acids

60.94

Weight (kDa)

5.57

Isoelectric Point (pI)

40.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 13 - 180 2.9e-55 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 241 - 480 1.7e-95 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 281 - 480 8e-55 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1187
AccI GTMKAC 1 cut(s) 8
AccII CGCG 1 cut(s) 183
AciI CCGC 1 cut(s) 1467
AclWI GGATC 1 cut(s) 1527
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 1 cut(s) 306
AcuI CTGAAG 1 cut(s) 552
AcyI GRCGYC 1 cut(s) 609
AdeI CACNNNGTG 1 cut(s) 620
AfaI GTAC 4 cut(s) 239, 476, 815, 892
AhlI ACTAGT 1 cut(s) 17
AluBI AGCT 6 cut(s) 351, 393, 501, 685, 1070, 1274
AluI AGCT 6 cut(s) 351, 393, 501, 685, 1070, 1274
Alw21I GWGCWC 2 cut(s) 483, 687
Alw26I GTCTC 5 cut(s) 382, 538, 753, 1097, 1388
AlwI GGATC 1 cut(s) 1527
AlwNI CAGNNNCTG 1 cut(s) 1169
AoxI GGCC 1 cut(s) 250
ApeKI GCWGC 2 cut(s) 55, 58
ApoI RAATTY 1 cut(s) 306
Asp700I GAANNNNTTC 1 cut(s) 977
AspLEI GCGC 1 cut(s) 578
AsuHPI GGTGA 2 cut(s) 125, 448
AsuII TTCGAA 1 cut(s) 553
BalI TGGCCA 1 cut(s) 252
BanII GRGCYC 1 cut(s) 687
BarI GAAGNNNNNNTAC 2 cut(s) 1506, 1538
BauI CACGAG 1 cut(s) 1550
BbsI GAAGAC 1 cut(s) 1045
Bbv12I GWGCWC 2 cut(s) 483, 687
BbvI GCAGC 2 cut(s) 67, 70
BccI CCATC 4 cut(s) 242, 261, 781, 1534
BciVI GTATCC 1 cut(s) 860
BclI TGATCA 1 cut(s) 226
BcoDI GTCTC 5 cut(s) 382, 538, 753, 1097, 1388
BcuI ACTAGT 1 cut(s) 17
BfaI CTAG 5 cut(s) 18, 698, 711, 728, 1350
BfmI CTRYAG 1 cut(s) 1161
BfuI GTATCC 1 cut(s) 860
BisI GCNGC 2 cut(s) 56, 59
BlpI GCTNAGC 1 cut(s) 389
BlsI GCNGC 2 cut(s) 57, 60
BmcAI AGTACT 1 cut(s) 815
BmrI ACTGGG 1 cut(s) 805
BmsI GCATC 4 cut(s) 34, 133, 871, 1222
BmuI ACTGGG 1 cut(s) 805
BpiI GAAGAC 1 cut(s) 1045
Bpu1102I GCTNAGC 1 cut(s) 389
Bpu14I TTCGAA 1 cut(s) 553
BpuEI CTTGAG 3 cut(s) 116, 455, 903
BsaAI YACGTR 1 cut(s) 478
BsaBI GATNNNNATC 3 cut(s) 231, 252, 654
BsaHI GRCGYC 1 cut(s) 609
BsaI GGTCTC 2 cut(s) 538, 1097
BsaWI WCCGGW 1 cut(s) 380
BsaXI ACNNNNNCTCC 2 cut(s) 1206, 1236
Bse118I RCCGGY 1 cut(s) 1480
Bse1I ACTGG 2 cut(s) 509, 811
Bse3DI GCAATG 1 cut(s) 496
Bse8I GATNNNNATC 3 cut(s) 231, 252, 654
BseGI GGATG 5 cut(s) 792, 874, 1237, 1258, 1306
BseJI GATNNNNATC 3 cut(s) 231, 252, 654
BseMI GCAATG 1 cut(s) 496
BseNI ACTGG 2 cut(s) 509, 811
BseRI GAGGAG 1 cut(s) 1463
BseXI GCAGC 2 cut(s) 67, 70
Bsh1236I CGCG 1 cut(s) 183
BshFI GGCC 1 cut(s) 252
BsiHKAI GWGCWC 2 cut(s) 483, 687
BsiSI CCGG 2 cut(s) 381, 1481
BsmAI GTCTC 5 cut(s) 382, 538, 753, 1097, 1388
BsmBI CGTCTC 1 cut(s) 1388
BsmI GAATGC 1 cut(s) 365
BsnI GGCC 1 cut(s) 252
Bso31I GGTCTC 2 cut(s) 538, 1097
Bsp119I TTCGAA 1 cut(s) 553
Bsp1286I GDGCHC 2 cut(s) 483, 687
Bsp143I GATC 6 cut(s) 115, 226, 232, 909, 1088, 1519
Bsp1720I GCTNAGC 1 cut(s) 389
BspACI CCGC 1 cut(s) 1467
BspANI GGCC 1 cut(s) 252
BspFNI CGCG 1 cut(s) 183
BspHI TCATGA 3 cut(s) 229, 906, 985
BspPI GGATC 1 cut(s) 1527
BspQI GCTCTTC 1 cut(s) 453
BspT104I TTCGAA 1 cut(s) 553
BspTNI GGTCTC 2 cut(s) 538, 1097
BsrDI GCAATG 1 cut(s) 496
BsrFI RCCGGY 1 cut(s) 1480
BsrI ACTGG 2 cut(s) 509, 811
BssAI RCCGGY 1 cut(s) 1480
BssMI GATC 6 cut(s) 115, 226, 232, 909, 1088, 1519
BssNI GRCGYC 1 cut(s) 609
BssSI CACGAG 1 cut(s) 1550
Bst2BI CACGAG 1 cut(s) 1550
Bst4CI ACNGT 4 cut(s) 82, 242, 620, 1165
Bst6I CTCTTC 4 cut(s) 83, 435, 453, 555
BstACI GRCGYC 1 cut(s) 609
BstAPI GCANNNNNTGC 1 cut(s) 1359
BstBAI YACGTR 1 cut(s) 478
BstBI TTCGAA 1 cut(s) 553
BstC8I GCNNGC 1 cut(s) 1341
BstDEI CTNAG 3 cut(s) 287, 389, 413
BstF5I GGATG 5 cut(s) 792, 874, 1237, 1258, 1306
BstFNI CGCG 1 cut(s) 183
BstHHI GCGC 1 cut(s) 578
BstKTI GATC 6 cut(s) 118, 229, 235, 912, 1091, 1522
BstMAI GTCTC 5 cut(s) 382, 538, 753, 1097, 1388
BstMBI GATC 6 cut(s) 115, 226, 232, 909, 1088, 1519
BstMWI GCNNNNNNNGC 3 cut(s) 1067, 1134, 1359
BstNSI RCATGY 1 cut(s) 1343
BstSFI CTRYAG 1 cut(s) 1161
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 2 cut(s) 67, 70
BstV2I GAAGAC 1 cut(s) 1045
BstX2I RGATCY 1 cut(s) 1519
BstXI CCANNNNNNTGG 1 cut(s) 158
BstYI RGATCY 1 cut(s) 1519
BsuI GTATCC 1 cut(s) 860
BsuRI GGCC 1 cut(s) 252
BtsCI GGATG 5 cut(s) 792, 874, 1237, 1258, 1306
BtsI GCAGTG 1 cut(s) 197
BtsIMutI CAGTG 2 cut(s) 197, 625
Cac8I GCNNGC 1 cut(s) 1341
CaiI CAGNNNCTG 1 cut(s) 1169
CciI TCATGA 3 cut(s) 229, 906, 985
CfoI GCGC 1 cut(s) 578
Cfr10I RCCGGY 1 cut(s) 1480
CseI GACGC 2 cut(s) 617, 1134
Csp6I GTAC 4 cut(s) 238, 475, 814, 891
CspCI CAANNNNNGTGG 6 cut(s) 495, 530, 1303, 1338, 1497, 1532
CviQI GTAC 4 cut(s) 238, 475, 814, 891
DdeI CTNAG 3 cut(s) 287, 389, 413
DpnI GATC 6 cut(s) 117, 228, 234, 911, 1090, 1521
DpnII GATC 6 cut(s) 115, 226, 232, 909, 1088, 1519
DraIII CACNNNGTG 1 cut(s) 620
EaeI YGGCCR 1 cut(s) 250
Eam1104I CTCTTC 4 cut(s) 83, 435, 453, 555
EarI CTCTTC 4 cut(s) 83, 435, 453, 555
Ecl136II GAGCTC 1 cut(s) 685
Eco24I GRGCYC 1 cut(s) 687
Eco31I GGTCTC 2 cut(s) 538, 1097
Eco32I GATATC 2 cut(s) 302, 874
Eco53kI GAGCTC 1 cut(s) 685
Eco57I CTGAAG 1 cut(s) 552
EcoICRI GAGCTC 1 cut(s) 685
EcoRV GATATC 2 cut(s) 302, 874
EcoT22I ATGCAT 3 cut(s) 886, 950, 1237
EcoT38I GRGCYC 1 cut(s) 687
Esp3I CGTCTC 1 cut(s) 1388
FalI AAGNNNNNCTT 6 cut(s) 102, 134, 420, 452, 432, 464
FauNDI CATATG 2 cut(s) 886, 1057
FbaI TGATCA 1 cut(s) 226
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 2 cut(s) 56, 59
FokI GGATG 5 cut(s) 799, 881, 1244, 1265, 1313
FriOI GRGCYC 1 cut(s) 687
Fsp4HI GCNGC 2 cut(s) 56, 59
FspBI CTAG 5 cut(s) 18, 698, 711, 728, 1350
GlaI GCGC 1 cut(s) 577
GluI GCNGC 2 cut(s) 56, 59
HaeIII GGCC 1 cut(s) 252
HapII CCGG 2 cut(s) 381, 1481
HgaI GACGC 2 cut(s) 617, 1134
HhaI GCGC 1 cut(s) 578
Hin1I GRCGYC 1 cut(s) 609
Hin6I GCGC 1 cut(s) 576
HinP1I GCGC 1 cut(s) 576
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HindIII AAGCTT 1 cut(s) 391
HinfI GANTC 7 cut(s) 129, 194, 338, 623, 828, 997, 1544
HpaII CCGG 2 cut(s) 381, 1481
HphI GGTGA 2 cut(s) 125, 448
Hpy166II GTNNAC 3 cut(s) 9, 67, 456
Hpy188I TCNGA 4 cut(s) 36, 316, 373, 1134
Hpy188III TCNNGA 5 cut(s) 224, 230, 688, 907, 986
Hpy8I GTNNAC 3 cut(s) 9, 67, 456
HpyAV CCTTC 8 cut(s) 31, 104, 511, 1028, 1115, 1222, 1356, 1429
HpyCH4III ACNGT 4 cut(s) 82, 242, 620, 1165
HpyCH4IV ACGT 2 cut(s) 477, 1404
HpyF10VI GCNNNNNNNGC 3 cut(s) 1067, 1134, 1359
HpyF3I CTNAG 3 cut(s) 287, 389, 413
HpySE526I ACGT 2 cut(s) 477, 1404
Hsp92I GRCGYC 1 cut(s) 609
HspAI GCGC 1 cut(s) 576
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 6 cut(s) 115, 226, 232, 909, 1088, 1519
LguI GCTCTTC 1 cut(s) 453
LmnI GCTCC 1 cut(s) 682
LpnPI CCDG 9 cut(s) 164, 394, 522, 748, 824, 916, 975, 1067, 1494
Lsp1109I GCAGC 2 cut(s) 67, 70
LweI GCATC 4 cut(s) 34, 133, 871, 1222
MaeI CTAG 5 cut(s) 18, 698, 711, 728, 1350
MaeII ACGT 2 cut(s) 477, 1404
MaeIII GTNAC 2 cut(s) 1171, 1222
MalI GATC 6 cut(s) 117, 228, 234, 911, 1090, 1521
MboI GATC 6 cut(s) 115, 226, 232, 909, 1088, 1519
MfeI CAATTG 1 cut(s) 924
MflI RGATCY 1 cut(s) 1519
MhlI GDGCHC 2 cut(s) 483, 687
MlsI TGGCCA 1 cut(s) 252
MluNI TGGCCA 1 cut(s) 252
MlyI GAGTC 1 cut(s) 123
MmeI TCCRAC 2 cut(s) 627, 1230
MnlI CCTC 9 cut(s) 84, 436, 558, 622, 766, 863, 1135, 1328, 1441
Mox20I TGGCCA 1 cut(s) 252
Mph1103I ATGCAT 3 cut(s) 886, 950, 1237
MroXI GAANNNNTTC 1 cut(s) 977
MscI TGGCCA 1 cut(s) 252
MseI TTAA 5 cut(s) 137, 365, 420, 1458, 1505
MslI CAYNNNNRTG 2 cut(s) 210, 1240
Msp20I TGGCCA 1 cut(s) 252
MspI CCGG 2 cut(s) 381, 1481
MunI CAATTG 1 cut(s) 924
Mva1269I GAATGC 1 cut(s) 365
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 3 cut(s) 1067, 1134, 1359
NdeI CATATG 2 cut(s) 886, 1057
NdeII GATC 6 cut(s) 115, 226, 232, 909, 1088, 1519
NsiI ATGCAT 3 cut(s) 886, 950, 1237
NspI RCATGY 1 cut(s) 1343
NspV TTCGAA 1 cut(s) 553
PaeI GCATGC 1 cut(s) 1343
PagI TCATGA 3 cut(s) 229, 906, 985
PciSI GCTCTTC 1 cut(s) 453
PctI GAATGC 1 cut(s) 365
PdmI GAANNNNTTC 1 cut(s) 977
PfeI GAWTC 6 cut(s) 194, 338, 623, 828, 997, 1544
PkrI GCNGC 2 cut(s) 57, 60
PleI GAGTC 1 cut(s) 123
PpsI GAGTC 1 cut(s) 123
Ppu21I YACGTR 1 cut(s) 478
PsiI TTATAA 1 cut(s) 1187
Psp124BI GAGCTC 1 cut(s) 687
PstNI CAGNNNCTG 1 cut(s) 1169
PsuI RGATCY 1 cut(s) 1519
RsaI GTAC 4 cut(s) 239, 476, 815, 892
RsaNI GTAC 4 cut(s) 238, 475, 814, 891
RseI CAYNNNNRTG 2 cut(s) 210, 1240
SacI GAGCTC 1 cut(s) 687
SapI GCTCTTC 1 cut(s) 453
SaqAI TTAA 5 cut(s) 137, 365, 420, 1458, 1505
SatI GCNGC 2 cut(s) 56, 59
Sau3AI GATC 6 cut(s) 115, 226, 232, 909, 1088, 1519
ScaI AGTACT 1 cut(s) 815
SchI GAGTC 1 cut(s) 123
SduI GDGCHC 2 cut(s) 483, 687
SfaNI GCATC 4 cut(s) 34, 133, 871, 1222
SfcI CTRYAG 1 cut(s) 1161
SfuI TTCGAA 1 cut(s) 553
SmiMI CAYNNNNRTG 2 cut(s) 210, 1240
SmlI CTYRAG 3 cut(s) 131, 470, 918
SmoI CTYRAG 3 cut(s) 131, 470, 918
SpeI ACTAGT 1 cut(s) 17
SphI GCATGC 1 cut(s) 1343
SsiI CCGC 1 cut(s) 1467
SspI AATATT 1 cut(s) 978
SspMI CTAG 5 cut(s) 18, 698, 711, 728, 1350
SstI GAGCTC 1 cut(s) 687
TaaI ACNGT 4 cut(s) 82, 242, 620, 1165
TaiI ACGT 2 cut(s) 480, 1407
TaqI TCGA 5 cut(s) 127, 553, 689, 1048, 1087
TatI WGTACW 1 cut(s) 813
TfiI GAWTC 6 cut(s) 194, 338, 623, 828, 997, 1544
Tru1I TTAA 5 cut(s) 137, 365, 420, 1458, 1505
Tru9I TTAA 5 cut(s) 137, 365, 420, 1458, 1505
TscAI CASTG 2 cut(s) 204, 625
TseI GCWGC 2 cut(s) 55, 58
TspRI CASTG 2 cut(s) 204, 625
XapI RAATTY 1 cut(s) 306
XceI RCATGY 1 cut(s) 1343
XcmI CCANNNNNNNNNTGG 1 cut(s) 818
XmiI GTMKAC 1 cut(s) 8
XmnI GAANNNNTTC 1 cut(s) 977
XspI CTAG 5 cut(s) 18, 698, 711, 728, 1350
ZrmI AGTACT 1 cut(s) 815
Zsp2I ATGCAT 3 cut(s) 886, 950, 1237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.