Rroxscaffold_1G00040400

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000053
Physical Location & Seq
Forward (+)
58071219 .. 58077710
6492 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00040400.1

Sequence Viewer

Length: 1632 bp
ATGTCTGAAATTGCTGGTCTGACAGCAAACTTTCAACCGGCCGGCATTTGCGGAGATGGGTTCATCAACTGTGATTTACTAGACAACGTAACTCGTGCCCATATGCAACAGCAAATCGACCGACTGAAAGTATTAGTGAGGGAAGGTTTTACAGTTTCATCTGGTTTATCCCATCAGCTGAAATTAATTGATGATATCCAGCGCCTAGGAGTGTCATCCCATTTTGAAACAGAAATAGAAGAAGCACTGGAACATATTCATGTTCAGTTGCATGATGTTGATCTGGACCTATACAATGACTCTCTTCGTTTTCGTCTATTAAGACAAAATGGATATAATGTTTCACCTGGAGATATATTCAACAAATTCAAAGATGCAAATGGTTACTTCAAGGAGAGCTTAATTGCCGATGTTCTGGGGATGCTAAGCTTGTATGAAGCAACGCATCTTAGGGTTCATGGAGAAGAAATACTAGAAGAAGCTCTGGTTTTCACCACCAATCACCTTAAGTTGGCGATAAGCCAGGTAAGCTATCCACTAACAGCACAGATATCTCAAGCCCTAGAAATACCTGTGCGGAGAGGTCTAGAGAGACTATCTGCCAGGAATTACATGTCAATCTACCAAGCCACGACTTCACATAATGAAGCATTACTGAAACTTGCAAAGTTAGATTTCAATTTGGTTCAATCTTTACACAAAGAGGAGCTTAGTGAGGTTACCAGATGGTGGAAAGAACTAGACTTTGAAAGGAACATCCCTTTTGTAAGGCATAGGATTGTGGAGTGTTTCTTTTGGATGGTGGGATTGTATTTTGAACCCCAATACTCGGTTGCGAGAAAGATTGCAACAAAACTGGGTGCTTTGGCTACAATTTTGGATGATATCTACGATTCATTTGCTACATTAGAAGAACTCAAGATATTTCGTGAAGCAATTGACAGGTGGGATGTCAACTATTGCATGAATGGCCTACCACGATACATGAAAGTATATTATCATTCACTTTTGAATATTATGAATGAAATTGAGGAAGAGCTGGAAAATCAAGGAATGTCATACCGACTCCATTATGCAAAACAAGTTTTTAAGGATCAAGCCAGAGACTATCTCGTTGAGGCCGAATGGCTCCACGAAGGATGTATCCCAAGCATGGAGGAGTACATGCCTATTAGATTAAGTTCTGTTGCTGCTTGTTTGACTATAGTCATATCTTTACTTGGAATGGGCGATGCTGTTACCAAGGAAACATTTGAGTGGGTTTTGAAGTACCCTAAAATTGTCAAGGCTTCGAGCCTCATTTTTAGGCTCATGGATGACATTGGGGGTAGCAAGTTTCCGAAACAGAAAGGGGACGTTGCTTCTAGTATTGATTGCTACATGAAACAATATGGGGTCTCAGATGAAGAGACAATCGATGTGTTTAACAAACAAATTGTAGATTCATGGAAAGAAATAAATGAGGATTTTCTTCGACCAACTGCTGTGCCAATGTCTGTGCTCATGCGTGTTCTTAATTTCACAAGAGTTGTTGATCTGATTTACAAAGAAGAAGATGGCTTTACACATGTTGGGAAAGTAGTAAAACATGGTGTCGCTGCACTTTTTGTTGATCCATTGCCACTCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

543

Amino Acids

62.41

Weight (kDa)

5.24

Isoelectric Point (pI)

44.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 30 - 188 3.3e-50 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 245 - 485 2.3e-92 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 285 - 485 1.7e-54 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 729
AciI CCGC 2 cut(s) 51, 577
AclWI GGATC 2 cut(s) 1101, 1607
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 1 cut(s) 365
AfaI GTAC 2 cut(s) 1163, 1271
AfiI CCNNNNNNNGG 4 cut(s) 511, 729, 829, 1153
AflII CTTAAG 1 cut(s) 506
AflIII ACRYGT 2 cut(s) 612, 1567
AjnI CCWGG 3 cut(s) 346, 522, 602
AluBI AGCT 7 cut(s) 178, 399, 429, 482, 531, 709, 1039
AluI AGCT 7 cut(s) 178, 399, 429, 482, 531, 709, 1039
Alw21I GWGCWC 1 cut(s) 1503
Alw26I GTCTC 4 cut(s) 586, 1098, 1402, 1403
AlwI GGATC 2 cut(s) 1101, 1607
AoxI GGCC 3 cut(s) 39, 970, 1119
ApeKI GCWGC 2 cut(s) 1190, 1598
ApoI RAATTY 1 cut(s) 365
AseI ATTAAT 1 cut(s) 185
Asp700I GAANNNNTTC 1 cut(s) 255
AspA2I CCTAGG 1 cut(s) 205
AspLEI GCGC 1 cut(s) 204
AspS9I GGNCC 1 cut(s) 286
AsuHPI GGTGA 3 cut(s) 336, 484, 494
AvaII GGWCC 1 cut(s) 286
AvrII CCTAGG 1 cut(s) 205
BaeGI GKGCMC 1 cut(s) 100
BauI CACGAG 1 cut(s) 93
Bbv12I GWGCWC 1 cut(s) 1503
BbvI GCAGC 2 cut(s) 1177, 1585
BccI CCATC 5 cut(s) 50, 180, 720, 793, 1550
BcgI CGANNNNNNTGC 2 cut(s) 881, 915
BciT130I CCWGG 3 cut(s) 348, 524, 604
BciVI GTATCC 1 cut(s) 1154
BcoDI GTCTC 4 cut(s) 586, 1098, 1402, 1403
BfaI CTAG 7 cut(s) 80, 206, 473, 563, 587, 740, 1365
BfmI CTRYAG 1 cut(s) 1203
BfoI RGCGCY 1 cut(s) 205
BfrI CTTAAG 1 cut(s) 506
BfuI GTATCC 1 cut(s) 1154
BisI GCNGC 2 cut(s) 1191, 1599
BlnI CCTAGG 1 cut(s) 205
BlpI GCTNAGC 1 cut(s) 425
BlsI GCNGC 2 cut(s) 1192, 1600
Bme1390I CCNGG 3 cut(s) 348, 524, 604
Bme18I GGWCC 1 cut(s) 286
BmgT120I GGNCC 1 cut(s) 286
BmiI GGNNCC 1 cut(s) 1130
BmrFI CCNGG 3 cut(s) 348, 524, 604
BmrI ACTGGG 1 cut(s) 866
BmsI GCATC 4 cut(s) 364, 411, 454, 1222
BmuI ACTGGG 1 cut(s) 866
BoxI GACNNNNGTC 1 cut(s) 1205
BplI GAGNNNNNCTC 2 cut(s) 1095, 1127
BpmI CTGGAG 1 cut(s) 369
Bpu1102I GCTNAGC 1 cut(s) 425
BpuEI CTTGAG 2 cut(s) 540, 902
Bsa29I ATCGAT 1 cut(s) 1416
BsaBI GATNNNNATC 1 cut(s) 279
BsaI GGTCTC 1 cut(s) 1402
BsaJI CCNNGG 2 cut(s) 205, 1242
Bsc4I CCNNNNNNNGG 4 cut(s) 511, 729, 829, 1153
Bse118I RCCGGY 2 cut(s) 37, 41
Bse1I ACTGG 2 cut(s) 252, 861
Bse3DI GCAATG 1 cut(s) 1616
Bse8I GATNNNNATC 1 cut(s) 279
BseBI CCWGG 3 cut(s) 348, 524, 604
BseCI ATCGAT 1 cut(s) 1416
BseDI CCNNGG 2 cut(s) 205, 1242
BseGI GGATG 8 cut(s) 215, 426, 756, 804, 886, 955, 1145, 1321
BseJI GATNNNNATC 1 cut(s) 279
BseLI CCNNNNNNNGG 4 cut(s) 511, 729, 829, 1153
BseMI GCAATG 1 cut(s) 1616
BseMII CTCAG 1 cut(s) 1413
BseNI ACTGG 2 cut(s) 252, 861
BseRI GAGGAG 2 cut(s) 719, 1172
BseSI GKGCMC 1 cut(s) 100
BseX3I CGGCCG 1 cut(s) 39
BseXI GCAGC 2 cut(s) 1177, 1585
BsgI GTGCAG 1 cut(s) 1584
Bsh1285I CGRYCG 2 cut(s) 42, 121
BshFI GGCC 3 cut(s) 41, 972, 1121
BshVI ATCGAT 1 cut(s) 1416
BsiEI CGRYCG 2 cut(s) 42, 121
BsiHKAI GWGCWC 1 cut(s) 1503
BsiSI CCGG 2 cut(s) 38, 42
BslFI GGGAC 1 cut(s) 1367
BslI CCNNNNNNNGG 4 cut(s) 511, 729, 829, 1153
BsmAI GTCTC 4 cut(s) 586, 1098, 1402, 1403
BsmFI GGGAC 1 cut(s) 1367
BsnI GGCC 3 cut(s) 41, 972, 1121
Bso31I GGTCTC 1 cut(s) 1402
Bsp1286I GDGCHC 2 cut(s) 100, 1503
Bsp143I GATC 4 cut(s) 280, 1093, 1534, 1612
Bsp1720I GCTNAGC 1 cut(s) 425
BspACI CCGC 2 cut(s) 51, 577
BspANI GGCC 3 cut(s) 41, 972, 1121
BspCNI CTCAG 1 cut(s) 1412
BspDI ATCGAT 1 cut(s) 1416
BspLI GGNNCC 1 cut(s) 1130
BspPI GGATC 2 cut(s) 1101, 1607
BspQI GCTCTTC 1 cut(s) 1029
BspTI CTTAAG 1 cut(s) 506
BspTNI GGTCTC 1 cut(s) 1402
BsrDI GCAATG 1 cut(s) 1616
BsrFI RCCGGY 2 cut(s) 37, 41
BsrI ACTGG 2 cut(s) 252, 861
BssAI RCCGGY 2 cut(s) 37, 41
BssECI CCNNGG 2 cut(s) 205, 1242
BssMI GATC 4 cut(s) 280, 1093, 1534, 1612
BssSI CACGAG 1 cut(s) 93
BssT1I CCWWGG 2 cut(s) 205, 1242
Bst2BI CACGAG 1 cut(s) 93
Bst2UI CCWGG 3 cut(s) 348, 524, 604
Bst4CI ACNGT 2 cut(s) 71, 154
Bst6I CTCTTC 3 cut(s) 309, 1029, 1401
BstAFI CTTAAG 1 cut(s) 506
BstC8I GCNNGC 1 cut(s) 43
BstDEI CTNAG 4 cut(s) 425, 449, 710, 1399
BstEII GGTNACC 1 cut(s) 718
BstF5I GGATG 8 cut(s) 215, 426, 756, 804, 886, 955, 1145, 1321
BstH2I RGCGCY 1 cut(s) 205
BstHHI GCGC 1 cut(s) 204
BstKTI GATC 4 cut(s) 283, 1096, 1537, 1615
BstMAI GTCTC 4 cut(s) 586, 1098, 1402, 1403
BstMBI GATC 4 cut(s) 280, 1093, 1534, 1612
BstMCI CGRYCG 2 cut(s) 42, 121
BstMWI GCNNNNNNNGC 2 cut(s) 528, 969
BstNI CCWGG 3 cut(s) 348, 524, 604
BstNSI RCATGY 3 cut(s) 616, 1168, 1571
BstPAI GACNNNNGTC 1 cut(s) 1205
BstPI GGTNACC 1 cut(s) 718
BstSCI CCNGG 3 cut(s) 346, 522, 602
BstSFI CTRYAG 1 cut(s) 1203
BstSLI GKGCMC 1 cut(s) 100
BstV1I GCAGC 2 cut(s) 1177, 1585
BstZI CGGCCG 1 cut(s) 39
Bsu15I ATCGAT 1 cut(s) 1416
BsuI GTATCC 1 cut(s) 1154
BsuRI GGCC 3 cut(s) 41, 972, 1121
BsuTUI ATCGAT 1 cut(s) 1416
BtgZI GCGATG 1 cut(s) 1245
BtsCI GGATG 8 cut(s) 215, 426, 756, 804, 886, 955, 1145, 1321
BtsIMutI CAGTG 1 cut(s) 245
Cac8I GCNNGC 1 cut(s) 43
CfoI GCGC 1 cut(s) 204
Cfr10I RCCGGY 2 cut(s) 37, 41
Cfr13I GGNCC 1 cut(s) 286
ClaI ATCGAT 1 cut(s) 1416
Csp6I GTAC 2 cut(s) 1162, 1270
CviQI GTAC 2 cut(s) 1162, 1270
DdeI CTNAG 4 cut(s) 425, 449, 710, 1399
DpnI GATC 4 cut(s) 282, 1095, 1536, 1614
DpnII GATC 4 cut(s) 280, 1093, 1534, 1612
EaeI YGGCCR 1 cut(s) 39
EagI CGGCCG 1 cut(s) 39
Eam1104I CTCTTC 3 cut(s) 309, 1029, 1401
EarI CTCTTC 3 cut(s) 309, 1029, 1401
EclXI CGGCCG 1 cut(s) 39
Eco130I CCWWGG 2 cut(s) 205, 1242
Eco31I GGTCTC 1 cut(s) 1402
Eco32I GATATC 3 cut(s) 196, 552, 886
Eco47I GGWCC 1 cut(s) 286
Eco52I CGGCCG 1 cut(s) 39
Eco91I GGTNACC 1 cut(s) 718
EcoO65I GGTNACC 1 cut(s) 718
EcoRII CCWGG 3 cut(s) 346, 522, 602
EcoRV GATATC 3 cut(s) 196, 552, 886
EcoT14I CCWWGG 2 cut(s) 205, 1242
ErhI CCWWGG 2 cut(s) 205, 1242
FalI AAGNNNNNCTT 4 cut(s) 383, 415, 693, 725
FaqI GGGAC 1 cut(s) 1367
FauNDI CATATG 1 cut(s) 102
Fnu4HI GCNGC 2 cut(s) 1191, 1599
FokI GGATG 8 cut(s) 202, 433, 743, 811, 893, 962, 1152, 1328
Fsp4HI GCNGC 2 cut(s) 1191, 1599
FspBI CTAG 7 cut(s) 80, 206, 473, 563, 587, 740, 1365
GlaI GCGC 1 cut(s) 203
GluI GCNGC 2 cut(s) 1191, 1599
GsuI CTGGAG 1 cut(s) 369
HaeII RGCGCY 1 cut(s) 205
HaeIII GGCC 3 cut(s) 41, 972, 1121
HapII CCGG 2 cut(s) 38, 42
HhaI GCGC 1 cut(s) 204
Hin6I GCGC 1 cut(s) 202
HinP1I GCGC 1 cut(s) 202
HincII GTYRAC 1 cut(s) 955
HindII GTYRAC 1 cut(s) 955
HindIII AAGCTT 1 cut(s) 427
HinfI GANTC 4 cut(s) 299, 893, 1065, 1442
HpaII CCGG 2 cut(s) 38, 42
HphI GGTGA 3 cut(s) 336, 484, 494
Hpy166II GTNNAC 1 cut(s) 955
Hpy188I TCNGA 5 cut(s) 7, 21, 1341, 1402, 1539
Hpy188III TCNNGA 4 cut(s) 284, 587, 919, 929
Hpy8I GTNNAC 1 cut(s) 955
HpyAV CCTTC 2 cut(s) 137, 1130
HpyCH4III ACNGT 2 cut(s) 71, 154
HpyCH4IV ACGT 2 cut(s) 87, 1356
HpyCH4V TGCA 8 cut(s) 106, 271, 377, 665, 848, 963, 1076, 1601
HpyF10VI GCNNNNNNNGC 2 cut(s) 528, 969
HpyF3I CTNAG 4 cut(s) 425, 449, 710, 1399
HpySE526I ACGT 2 cut(s) 87, 1356
HspAI GCGC 1 cut(s) 202
KroI GCCGGC 1 cut(s) 41
KroNI GCCGGC 1 cut(s) 43
Kzo9I GATC 4 cut(s) 280, 1093, 1534, 1612
LguI GCTCTTC 1 cut(s) 1029
LmnI GCTCC 2 cut(s) 706, 1134
Lsp1109I GCAGC 2 cut(s) 1177, 1585
LweI GCATC 4 cut(s) 364, 411, 454, 1222
MaeI CTAG 7 cut(s) 80, 206, 473, 563, 587, 740, 1365
MaeII ACGT 2 cut(s) 87, 1356
MaeIII GTNAC 4 cut(s) 88, 383, 718, 1237
MalI GATC 4 cut(s) 282, 1095, 1536, 1614
MboI GATC 4 cut(s) 280, 1093, 1534, 1612
MfeI CAATTG 1 cut(s) 936
MhlI GDGCHC 2 cut(s) 100, 1503
MlyI GAGTC 2 cut(s) 293, 1059
MnlI CCTC 9 cut(s) 132, 575, 697, 709, 1024, 1111, 1150, 1307, 1456
MroNI GCCGGC 1 cut(s) 41
MroXI GAANNNNTTC 1 cut(s) 255
MseI TTAA 8 cut(s) 185, 320, 401, 507, 1089, 1178, 1425, 1515
MslI CAYNNNNRTG 3 cut(s) 258, 1255, 1627
MspA1I CMGCKG 1 cut(s) 178
MspCI CTTAAG 1 cut(s) 506
MspI CCGG 2 cut(s) 38, 42
MspR9I CCNGG 3 cut(s) 348, 524, 604
MunI CAATTG 1 cut(s) 936
MvaI CCWGG 3 cut(s) 348, 524, 604
MwoI GCNNNNNNNGC 2 cut(s) 528, 969
NaeI GCCGGC 1 cut(s) 43
NdeI CATATG 1 cut(s) 102
NdeII GATC 4 cut(s) 280, 1093, 1534, 1612
NgoMIV GCCGGC 1 cut(s) 41
NlaIV GGNNCC 1 cut(s) 1130
NspI RCATGY 3 cut(s) 616, 1168, 1571
PciI ACATGT 2 cut(s) 612, 1567
PciSI GCTCTTC 1 cut(s) 1029
PcsI WCGNNNNNNNCGW 1 cut(s) 1119
PdiI GCCGGC 1 cut(s) 43
PdmI GAANNNNTTC 1 cut(s) 255
PfeI GAWTC 2 cut(s) 893, 1442
PflMI CCANNNNNTGG 1 cut(s) 729
PkrI GCNGC 2 cut(s) 1192, 1600
PleI GAGTC 2 cut(s) 293, 1059
PpsI GAGTC 2 cut(s) 293, 1059
PscI ACATGT 2 cut(s) 612, 1567
PshAI GACNNNNGTC 1 cut(s) 1205
PshBI ATTAAT 1 cut(s) 185
Psp6I CCWGG 3 cut(s) 346, 522, 602
PspEI GGTNACC 1 cut(s) 718
PspGI CCWGG 3 cut(s) 346, 522, 602
PspN4I GGNNCC 1 cut(s) 1130
PspPI GGNCC 1 cut(s) 286
PvuII CAGCTG 1 cut(s) 178
RsaI GTAC 2 cut(s) 1163, 1271
RsaNI GTAC 2 cut(s) 1162, 1270
RseI CAYNNNNRTG 3 cut(s) 258, 1255, 1627
SapI GCTCTTC 1 cut(s) 1029
SaqAI TTAA 8 cut(s) 185, 320, 401, 507, 1089, 1178, 1425, 1515
SatI GCNGC 2 cut(s) 1191, 1599
Sau3AI GATC 4 cut(s) 280, 1093, 1534, 1612
Sau96I GGNCC 1 cut(s) 286
SchI GAGTC 2 cut(s) 293, 1059
ScrFI CCNGG 3 cut(s) 348, 524, 604
SduI GDGCHC 2 cut(s) 100, 1503
SfaNI GCATC 4 cut(s) 364, 411, 454, 1222
SfcI CTRYAG 1 cut(s) 1203
SinI GGWCC 1 cut(s) 286
SmiMI CAYNNNNRTG 3 cut(s) 258, 1255, 1627
SmlI CTYRAG 3 cut(s) 506, 555, 917
SmoI CTYRAG 3 cut(s) 506, 555, 917
SsiI CCGC 2 cut(s) 51, 577
SspI AATATT 1 cut(s) 1015
SspMI CTAG 7 cut(s) 80, 206, 473, 563, 587, 740, 1365
StyD4I CCNGG 3 cut(s) 346, 522, 602
StyI CCWWGG 2 cut(s) 205, 1242
TaaI ACNGT 2 cut(s) 71, 154
TaiI ACGT 2 cut(s) 90, 1359
TaqI TCGA 5 cut(s) 117, 1292, 1416, 1474, 1626
TaqII GACCGA 1 cut(s) 135
TatI WGTACW 1 cut(s) 1161
TfiI GAWTC 2 cut(s) 893, 1442
Tru1I TTAA 8 cut(s) 185, 320, 401, 507, 1089, 1178, 1425, 1515
Tru9I TTAA 8 cut(s) 185, 320, 401, 507, 1089, 1178, 1425, 1515
TscAI CASTG 1 cut(s) 252
TseI GCWGC 2 cut(s) 1190, 1598
TspRI CASTG 1 cut(s) 252
Van91I CCANNNNNTGG 1 cut(s) 729
Vha464I CTTAAG 1 cut(s) 506
VpaK11BI GGWCC 1 cut(s) 286
VspI ATTAAT 1 cut(s) 185
XapI RAATTY 1 cut(s) 365
XbaI TCTAGA 1 cut(s) 586
XceI RCATGY 3 cut(s) 616, 1168, 1571
XmaJI CCTAGG 1 cut(s) 205
XmnI GAANNNNTTC 1 cut(s) 255
XspI CTAG 7 cut(s) 80, 206, 473, 563, 587, 740, 1365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.