synthase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 381 |
| Acc16I | TGCGCA | 1 cut(s) | 1089 |
| AccB1I | GGYRCC | 1 cut(s) | 1436 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 99 |
| AccBSI | CCGCTC | 1 cut(s) | 616 |
| AccI | GTMKAC | 2 cut(s) | 663, 834 |
| AccII | CGCG | 1 cut(s) | 1241 |
| AciI | CCGC | 4 cut(s) | 614, 909, 1177, 1524 |
| AcsI | RAATTY | 4 cut(s) | 291, 303, 428, 1071 |
| AcuI | CTGAAG | 2 cut(s) | 60, 999 |
| AdeI | CACNNNGTG | 1 cut(s) | 771 |
| AfaI | GTAC | 4 cut(s) | 162, 338, 1208, 1586 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 99, 537, 885, 1299 |
| AflIII | ACRYGT | 3 cut(s) | 157, 1209, 1239 |
| AjiI | CACGTC | 1 cut(s) | 790 |
| AjnI | CCWGG | 2 cut(s) | 536, 666 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1382, 1414 |
| Alw21I | GWGCWC | 2 cut(s) | 317, 972 |
| Alw26I | GTCTC | 3 cut(s) | 810, 1006, 1408 |
| Ama87I | CYCGRG | 1 cut(s) | 1566 |
| AoxI | GGCC | 1 cut(s) | 65 |
| ApeKI | GCWGC | 2 cut(s) | 344, 1331 |
| ApoI | RAATTY | 4 cut(s) | 291, 303, 428, 1071 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1361, 1393 |
| AspLEI | GCGC | 1 cut(s) | 1090 |
| AspS9I | GGNCC | 1 cut(s) | 1369 |
| AsuHPI | GGTGA | 4 cut(s) | 116, 431, 526, 675 |
| AvaI | CYCGRG | 1 cut(s) | 1566 |
| AvaII | GGWCC | 1 cut(s) | 1369 |
| AxyI | CCTNAGG | 1 cut(s) | 491 |
| BanI | GGYRCC | 1 cut(s) | 1436 |
| BanII | GRGCYC | 2 cut(s) | 317, 972 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 279, 311 |
| BauI | CACGAG | 1 cut(s) | 1091 |
| Bbv12I | GWGCWC | 2 cut(s) | 317, 972 |
| BbvI | GCAGC | 2 cut(s) | 331, 1318 |
| BccI | CCATC | 5 cut(s) | 416, 750, 987, 1016, 1201 |
| BciT130I | CCWGG | 2 cut(s) | 538, 668 |
| BciVI | GTATCC | 1 cut(s) | 489 |
| BclI | TGATCA | 1 cut(s) | 106 |
| BcoDI | GTCTC | 3 cut(s) | 810, 1006, 1408 |
| BfaI | CTAG | 3 cut(s) | 515, 885, 965 |
| BfmI | CTRYAG | 2 cut(s) | 912, 1215 |
| BfuI | GTATCC | 1 cut(s) | 489 |
| BglII | AGATCT | 1 cut(s) | 1630 |
| BisI | GCNGC | 2 cut(s) | 345, 1332 |
| BlsI | GCNGC | 2 cut(s) | 346, 1333 |
| Bme1390I | CCNGG | 2 cut(s) | 538, 668 |
| Bme18I | GGWCC | 1 cut(s) | 1369 |
| BmeT110I | CYCGRG | 1 cut(s) | 1566 |
| BmgBI | CACGTC | 1 cut(s) | 790 |
| BmgT120I | GGNCC | 1 cut(s) | 1369 |
| BmiI | GGNNCC | 2 cut(s) | 1438, 1656 |
| BmrFI | CCNGG | 2 cut(s) | 538, 668 |
| BmsI | GCATC | 6 cut(s) | 130, 180, 926, 931, 1119, 1343 |
| BpmI | CTGGAG | 1 cut(s) | 865 |
| Bpu10I | CCTNAGC | 2 cut(s) | 69, 1614 |
| BpuEI | CTTGAG | 2 cut(s) | 569, 1302 |
| Bsa29I | ATCGAT | 1 cut(s) | 1011 |
| BsaBI | GATNNNNATC | 1 cut(s) | 60 |
| BsaI | GGTCTC | 1 cut(s) | 1408 |
| BsaJI | CCNNGG | 2 cut(s) | 632, 1659 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 73, 103 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 99, 537, 885, 1299 |
| Bse21I | CCTNAGG | 1 cut(s) | 491 |
| Bse8I | GATNNNNATC | 1 cut(s) | 60 |
| BseBI | CCWGG | 2 cut(s) | 538, 668 |
| BseCI | ATCGAT | 1 cut(s) | 1011 |
| BseDI | CCNNGG | 2 cut(s) | 632, 1659 |
| BseJI | GATNNNNATC | 1 cut(s) | 60 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 99, 537, 885, 1299 |
| BseMII | CTCAG | 2 cut(s) | 1017, 1419 |
| BseRI | GAGGAG | 1 cut(s) | 521 |
| BseXI | GCAGC | 2 cut(s) | 331, 1318 |
| BseYI | CCCAGC | 1 cut(s) | 92 |
| Bsh1236I | CGCG | 1 cut(s) | 1241 |
| BshFI | GGCC | 1 cut(s) | 67 |
| BshNI | GGYRCC | 1 cut(s) | 1436 |
| BshVI | ATCGAT | 1 cut(s) | 1011 |
| BsiHKAI | GWGCWC | 2 cut(s) | 317, 972 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1566 |
| BslFI | GGGAC | 1 cut(s) | 1514 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 99, 537, 885, 1299 |
| BsmAI | GTCTC | 3 cut(s) | 810, 1006, 1408 |
| BsmFI | GGGAC | 1 cut(s) | 1514 |
| BsnI | GGCC | 1 cut(s) | 67 |
| Bso31I | GGTCTC | 1 cut(s) | 1408 |
| BsoBI | CYCGRG | 1 cut(s) | 1566 |
| Bsp1286I | GDGCHC | 2 cut(s) | 317, 972 |
| Bsp1407I | TGTACA | 3 cut(s) | 160, 336, 1584 |
| Bsp143I | GATC | 3 cut(s) | 106, 1012, 1630 |
| BspACI | CCGC | 4 cut(s) | 614, 909, 1177, 1524 |
| BspANI | GGCC | 1 cut(s) | 67 |
| BspCNI | CTCAG | 2 cut(s) | 1016, 1418 |
| BspDI | ATCGAT | 1 cut(s) | 1011 |
| BspFNI | CGCG | 1 cut(s) | 1241 |
| BspHI | TCATGA | 1 cut(s) | 1075 |
| BspLI | GGNNCC | 2 cut(s) | 1438, 1656 |
| BspQI | GCTCTTC | 1 cut(s) | 354 |
| BspT107I | GGYRCC | 1 cut(s) | 1436 |
| BspTNI | GGTCTC | 1 cut(s) | 1408 |
| BsrBI | CCGCTC | 1 cut(s) | 616 |
| BsrGI | TGTACA | 3 cut(s) | 160, 336, 1584 |
| BssECI | CCNNGG | 2 cut(s) | 632, 1659 |
| BssMI | GATC | 3 cut(s) | 106, 1012, 1630 |
| BssSI | CACGAG | 1 cut(s) | 1091 |
| Bst2BI | CACGAG | 1 cut(s) | 1091 |
| Bst2UI | CCWGG | 2 cut(s) | 538, 668 |
| Bst4CI | ACNGT | 4 cut(s) | 419, 1067, 1342, 1418 |
| Bst6I | CTCTTC | 1 cut(s) | 354 |
| BstAUI | TGTACA | 3 cut(s) | 160, 336, 1584 |
| BstC8I | GCNNGC | 2 cut(s) | 1086, 1510 |
| BstDSI | CCRYGG | 1 cut(s) | 1659 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 535, 883 |
| BstFNI | CGCG | 1 cut(s) | 1241 |
| BstHHI | GCGC | 1 cut(s) | 1090 |
| BstKTI | GATC | 3 cut(s) | 109, 1015, 1633 |
| BstMAI | GTCTC | 3 cut(s) | 810, 1006, 1408 |
| BstMBI | GATC | 3 cut(s) | 106, 1012, 1630 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 799, 1127, 1328 |
| BstNI | CCWGG | 2 cut(s) | 538, 668 |
| BstNSI | RCATGY | 2 cut(s) | 161, 1213 |
| BstSCI | CCNGG | 2 cut(s) | 536, 666 |
| BstSFI | CTRYAG | 2 cut(s) | 912, 1215 |
| BstUI | CGCG | 1 cut(s) | 1241 |
| BstV1I | GCAGC | 2 cut(s) | 331, 1318 |
| BstX2I | RGATCY | 1 cut(s) | 1630 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1354 |
| BstYI | RGATCY | 1 cut(s) | 1630 |
| Bsu15I | ATCGAT | 1 cut(s) | 1011 |
| Bsu36I | CCTNAGG | 1 cut(s) | 491 |
| BsuI | GTATCC | 1 cut(s) | 489 |
| BsuRI | GGCC | 1 cut(s) | 67 |
| BsuTUI | ATCGAT | 1 cut(s) | 1011 |
| BtgI | CCRYGG | 1 cut(s) | 1659 |
| BtgZI | GCGATG | 1 cut(s) | 1145 |
| BtrI | CACGTC | 1 cut(s) | 790 |
| BtsI | GCAGTG | 2 cut(s) | 339, 1329 |
| BtsIMutI | CAGTG | 3 cut(s) | 339, 1072, 1329 |
| Cac8I | GCNNGC | 2 cut(s) | 1086, 1510 |
| CciI | TCATGA | 1 cut(s) | 1075 |
| CfoI | GCGC | 1 cut(s) | 1090 |
| Cfr13I | GGNCC | 1 cut(s) | 1369 |
| ClaI | ATCGAT | 1 cut(s) | 1011 |
| Csp6I | GTAC | 4 cut(s) | 161, 337, 1207, 1585 |
| CviQI | GTAC | 4 cut(s) | 161, 337, 1207, 1585 |
| DpnI | GATC | 3 cut(s) | 108, 1014, 1632 |
| DpnII | GATC | 3 cut(s) | 106, 1012, 1630 |
| DraIII | CACNNNGTG | 1 cut(s) | 771 |
| Eam1104I | CTCTTC | 1 cut(s) | 354 |
| EarI | CTCTTC | 1 cut(s) | 354 |
| EciI | GGCGGA | 1 cut(s) | 1166 |
| Ecl136II | GAGCTC | 2 cut(s) | 315, 970 |
| Eco24I | GRGCYC | 2 cut(s) | 317, 972 |
| Eco31I | GGTCTC | 1 cut(s) | 1408 |
| Eco47I | GGWCC | 1 cut(s) | 1369 |
| Eco53kI | GAGCTC | 2 cut(s) | 315, 970 |
| Eco57I | CTGAAG | 2 cut(s) | 60, 999 |
| Eco81I | CCTNAGG | 1 cut(s) | 491 |
| Eco88I | CYCGRG | 1 cut(s) | 1566 |
| EcoICRI | GAGCTC | 2 cut(s) | 315, 970 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 535, 883 |
| EcoRII | CCWGG | 2 cut(s) | 536, 666 |
| EcoT22I | ATGCAT | 1 cut(s) | 1489 |
| EcoT38I | GRGCYC | 2 cut(s) | 317, 972 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 215, 247 |
| FaqI | GGGAC | 1 cut(s) | 1514 |
| FauNDI | CATATG | 1 cut(s) | 121 |
| FbaI | TGATCA | 1 cut(s) | 106 |
| FblI | GTMKAC | 2 cut(s) | 663, 834 |
| Fnu4HI | GCNGC | 2 cut(s) | 345, 1332 |
| FriOI | GRGCYC | 2 cut(s) | 317, 972 |
| Fsp4HI | GCNGC | 2 cut(s) | 345, 1332 |
| FspBI | CTAG | 3 cut(s) | 515, 885, 965 |
| FspI | TGCGCA | 1 cut(s) | 1089 |
| GlaI | GCGC | 1 cut(s) | 1089 |
| GluI | GCNGC | 2 cut(s) | 345, 1332 |
| GsaI | CCCAGC | 1 cut(s) | 96 |
| GsuI | CTGGAG | 1 cut(s) | 865 |
| HaeIII | GGCC | 1 cut(s) | 67 |
| HhaI | GCGC | 1 cut(s) | 1090 |
| Hin6I | GCGC | 1 cut(s) | 1088 |
| HinP1I | GCGC | 1 cut(s) | 1088 |
| HincII | GTYRAC | 1 cut(s) | 1308 |
| HindII | GTYRAC | 1 cut(s) | 1308 |
| HindIII | AAGCTT | 2 cut(s) | 469, 1082 |
| HinfI | GANTC | 5 cut(s) | 437, 551, 680, 738, 1289 |
| HpaI | GTTAAC | 1 cut(s) | 1308 |
| HphI | GGTGA | 4 cut(s) | 116, 431, 526, 675 |
| Hpy166II | GTNNAC | 8 cut(s) | 339, 629, 664, 835, 1308, 1369, 1414, 1585 |
| Hpy188I | TCNGA | 2 cut(s) | 620, 747 |
| Hpy188III | TCNNGA | 3 cut(s) | 548, 1076, 1225 |
| Hpy8I | GTNNAC | 8 cut(s) | 339, 629, 664, 835, 1308, 1369, 1414, 1585 |
| HpyAV | CCTTC | 7 cut(s) | 84, 166, 180, 541, 772, 1321, 1486 |
| HpyCH4III | ACNGT | 4 cut(s) | 419, 1067, 1342, 1418 |
| HpyCH4IV | ACGT | 1 cut(s) | 789 |
| HpyCH4V | TGCA | 9 cut(s) | 458, 584, 733, 812, 900, 1049, 1121, 1334, 1487 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 799, 1127, 1328 |
| HpySE526I | ACGT | 1 cut(s) | 789 |
| HspAI | GCGC | 1 cut(s) | 1088 |
| Ksp22I | TGATCA | 1 cut(s) | 106 |
| KspAI | GTTAAC | 1 cut(s) | 1308 |
| Kzo9I | GATC | 3 cut(s) | 106, 1012, 1630 |
| LguI | GCTCTTC | 1 cut(s) | 354 |
| LmnI | GCTCC | 1 cut(s) | 621 |
| Lsp1109I | GCAGC | 2 cut(s) | 331, 1318 |
| LweI | GCATC | 6 cut(s) | 130, 180, 926, 931, 1119, 1343 |
| MaeI | CTAG | 3 cut(s) | 515, 885, 965 |
| MaeII | ACGT | 1 cut(s) | 789 |
| MaeIII | GTNAC | 5 cut(s) | 438, 592, 904, 1225, 1418 |
| MalI | GATC | 3 cut(s) | 108, 1014, 1632 |
| MbiI | CCGCTC | 1 cut(s) | 616 |
| MboI | GATC | 3 cut(s) | 106, 1012, 1630 |
| MboII | GAAGA | 8 cut(s) | 24, 188, 299, 341, 971, 1147, 1286, 1460 |
| MflI | RGATCY | 1 cut(s) | 1630 |
| MhlI | GDGCHC | 2 cut(s) | 317, 972 |
| MluI | ACGCGT | 1 cut(s) | 1239 |
| MlyI | GAGTC | 4 cut(s) | 446, 560, 747, 1283 |
| MmeI | TCCRAC | 2 cut(s) | 696, 1246 |
| Mph1103I | ATGCAT | 1 cut(s) | 1489 |
| MseI | TTAA | 3 cut(s) | 569, 605, 1307 |
| MslI | CAYNNNNRTG | 1 cut(s) | 255 |
| MspA1I | CMGCKG | 2 cut(s) | 1409, 1526 |
| MspR9I | CCNGG | 2 cut(s) | 538, 668 |
| MvaI | CCWGG | 2 cut(s) | 538, 668 |
| MvnI | CGCG | 1 cut(s) | 1241 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 799, 1127, 1328 |
| NdeI | CATATG | 1 cut(s) | 121 |
| NdeII | GATC | 3 cut(s) | 106, 1012, 1630 |
| NlaIV | GGNNCC | 2 cut(s) | 1438, 1656 |
| NmuCI | GTSAC | 2 cut(s) | 438, 1225 |
| NsbI | TGCGCA | 1 cut(s) | 1089 |
| NsiI | ATGCAT | 1 cut(s) | 1489 |
| NspI | RCATGY | 2 cut(s) | 161, 1213 |
| PagI | TCATGA | 1 cut(s) | 1075 |
| PciI | ACATGT | 2 cut(s) | 157, 1209 |
| PciSI | GCTCTTC | 1 cut(s) | 354 |
| PfeI | GAWTC | 1 cut(s) | 680 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 99 |
| PkrI | GCNGC | 2 cut(s) | 346, 1333 |
| PleI | GAGTC | 4 cut(s) | 445, 559, 746, 1283 |
| PpsI | GAGTC | 4 cut(s) | 445, 559, 746, 1283 |
| PscI | ACATGT | 2 cut(s) | 157, 1209 |
| PsiI | TTATAA | 1 cut(s) | 381 |
| Psp124BI | GAGCTC | 2 cut(s) | 317, 972 |
| Psp6I | CCWGG | 2 cut(s) | 536, 666 |
| PspFI | CCCAGC | 1 cut(s) | 92 |
| PspGI | CCWGG | 2 cut(s) | 536, 666 |
| PspN4I | GGNNCC | 2 cut(s) | 1438, 1656 |
| PspPI | GGNCC | 1 cut(s) | 1369 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 144, 176 |
| PsuI | RGATCY | 1 cut(s) | 1630 |
| PvuII | CAGCTG | 1 cut(s) | 1409 |
| RsaI | GTAC | 4 cut(s) | 162, 338, 1208, 1586 |
| RsaNI | GTAC | 4 cut(s) | 161, 337, 1207, 1585 |
| RseI | CAYNNNNRTG | 1 cut(s) | 255 |
| SacI | GAGCTC | 2 cut(s) | 317, 972 |
| SapI | GCTCTTC | 1 cut(s) | 354 |
| SaqAI | TTAA | 3 cut(s) | 569, 605, 1307 |
| SatI | GCNGC | 2 cut(s) | 345, 1332 |
| Sau3AI | GATC | 3 cut(s) | 106, 1012, 1630 |
| Sau96I | GGNCC | 1 cut(s) | 1369 |
| SchI | GAGTC | 4 cut(s) | 446, 560, 747, 1283 |
| ScrFI | CCNGG | 2 cut(s) | 538, 668 |
| SduI | GDGCHC | 2 cut(s) | 317, 972 |
| SfaNI | GCATC | 6 cut(s) | 130, 180, 926, 931, 1119, 1343 |
| SfcI | CTRYAG | 2 cut(s) | 912, 1215 |
| SinI | GGWCC | 1 cut(s) | 1369 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 255 |
| SmlI | CTYRAG | 2 cut(s) | 548, 1317 |
| SmoI | CTYRAG | 2 cut(s) | 548, 1317 |
| SsiI | CCGC | 4 cut(s) | 614, 909, 1177, 1524 |
| SspI | AATATT | 1 cut(s) | 875 |
| SspMI | CTAG | 3 cut(s) | 515, 885, 965 |
| SstI | GAGCTC | 2 cut(s) | 317, 972 |
| StyD4I | CCNGG | 2 cut(s) | 536, 666 |
| TaaI | ACNGT | 4 cut(s) | 419, 1067, 1342, 1418 |
| TaiI | ACGT | 1 cut(s) | 792 |
| TaqI | TCGA | 4 cut(s) | 358, 1011, 1158, 1461 |
| TatI | WGTACW | 4 cut(s) | 160, 336, 1206, 1584 |
| TfiI | GAWTC | 1 cut(s) | 680 |
| Tru1I | TTAA | 3 cut(s) | 569, 605, 1307 |
| Tru9I | TTAA | 3 cut(s) | 569, 605, 1307 |
| TscAI | CASTG | 3 cut(s) | 346, 1072, 1329 |
| TseFI | GTSAC | 2 cut(s) | 438, 1225 |
| TseI | GCWGC | 2 cut(s) | 344, 1331 |
| Tsp45I | GTSAC | 2 cut(s) | 438, 1225 |
| TspGWI | ACGGA | 1 cut(s) | 554 |
| TspRI | CASTG | 3 cut(s) | 346, 1072, 1329 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 99 |
| VpaK11BI | GGWCC | 1 cut(s) | 1369 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 535, 883 |
| XapI | RAATTY | 4 cut(s) | 291, 303, 428, 1071 |
| XceI | RCATGY | 2 cut(s) | 161, 1213 |
| XmiI | GTMKAC | 2 cut(s) | 663, 834 |
| XspI | CTAG | 3 cut(s) | 515, 885, 965 |
| Zsp2I | ATGCAT | 1 cut(s) | 1489 |