Prupe.4G200100_v2.0.a1

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000053
Physical Location & Seq
Forward (+)
12426875 .. 12431449
4575 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G200100.2

Sequence Viewer

Length: 1692 bp
ATGTCGAAAGAAGCTGGTTCAGCATCTCAATCAAAAAATCCTAAGCCTGATCAAATTGTTCGACGGACAGCAAACTACCAACCGAGCATTTGGGGGGATCAGTTTATCAATTATGATTCTGAAGACATTATAAATTATGCCCATAAGCAAGAAGAAGTTGATGAACTGAAACTAGTAGTGAGGAGGGAAGTGTTCACAACCAGTGGAGATGATTTTTCGAATCAGATGAAGCTAATTGATGCAATCCAGCGGCTCGGCGTGGCGTACCGCTTTGAAAAGGAAATAGAAGAAGCACTGGAACATATATATGCTGCAAATCATTTCCATGATGATGATGGTGATGGTGACCTATACGATGTTTCCCTTGGTTTTCGGCTACTAAGACAACATGGACATTATGTTTCATGCAGAATATTCAACAAGTTCAAAGATAGTAAAAATGGAGGCTTCAAGGAAAGCTTAATCGCTGATGTGCCAGGAATGCTAAGCTTTTATGAAGCAACGCATCTGAGGGTGCATGGAGAAGATATACTAGAAGAGGCTATTGTTTTCACCACAAAACATCTGGAGTCAGCAACAACCCATGTAAGCTATGAACTGGCAGAACAAATAGCTCAAGCCCTAGAGAGACCGCTCCGAAAGAGTCTCGAGAGGTTATGTGCCCGTCAGTTCATGTCCATCTACCAAGATGAAGCTTCACATAATGAAGCTCTATTAAAACTTGCCAAGTCAGATTTCAATCTTGTTCAGTCTTTGCACAAACAGGAGCTCAGTGAGATTATTAGGTGGTGGAAACAACTAGACTTTGGAAGGAAGCTGCCTTTTGCAAGAAACAGAATTGTGGAGTTATACTGCTGGGTTTTGGGGGTCTATTTTGAGCCCCAATACCTTTTTGGAAGAAAATTTCTCACAAAAATTATTGCCCTGCTTTGTGTAATGGATGATATCTATGACGCATTCGGTACATTTGAAGAACTTGAGATCTTTACCGAAGCAATTCATCAAAGATGGCATGCCAATTGCATGGATGGACTGCGAGACTACATGCAAATATTCTTCCATGCACTTTTGAATGTTTTCAATGAAATTGAGGAGGAGATGGTGAAGGAAGGAAGAGCATACCGAGCTCACTATGCAAAAGAAGCTTGGAAAACTAATGCCAAAGCTTACTTTGATGAGGCCAAATGGTTCCACGAAGGATGCATCCCCAGCATGGAGGAATATATGCGTGTTGCTACAGCATCTGCTGGGCACACCACACTTACAACTATGTCTTTACTTGGCATGGGAGACATTGTAACCAAGGAGTCCTTTGAGTGGTTGTTGAATGACCCTAAAATTCTTAGAGCTTCCAATATCATTGGTAGGCTCATGGATGACATTGTTTCGAGCAAGTTTGAGAAAGAGAGAGGGCATGTTGCTTCTGCCATTGATTGTTACAGGAAGCAATATGAGGTGTCAGATGAGCAGGAAATAATTGATGCCTTCAACAAACAGATTGTGGATTCGTGGAAAGATATCAACGAGGAGTTCCTCAGACCAACCTCTGTGCCAATGCCTATCCTTGTACGTGTTCTTAATTTAACAAGAGTGGCGGATCTCATCTACAAAAAAGATGATGGCCTCACACATGTTGGAAAAGTCATGAAAGATAGTGTTGCTTCTTATTTCATTGATCCGGCACCACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

564

Amino Acids

65.25

Weight (kDa)

5.38

Isoelectric Point (pI)

47.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 131
AccB1I GGYRCC 1 cut(s) 1681
AccBSI CCGCTC 1 cut(s) 634
AciI CCGC 4 cut(s) 250, 268, 632, 1595
AclWI GGATC 3 cut(s) 105, 1605, 1670
AcsI RAATTY 2 cut(s) 902, 1338
AcuI CTGAAG 1 cut(s) 141
AfaI GTAC 3 cut(s) 266, 964, 1569
AfiI CCNNNNNNNGG 2 cut(s) 1213, 1317
AflIII ACRYGT 2 cut(s) 1570, 1630
AhlI ACTAGT 1 cut(s) 172
AjnI CCWGG 1 cut(s) 475
AjuI GAANNNNNNNTTGG 2 cut(s) 719, 751
Alw21I GWGCWC 2 cut(s) 771, 1129
Alw26I GTCTC 4 cut(s) 622, 650, 1032, 1284
AlwI GGATC 3 cut(s) 105, 1605, 1670
AlwNI CAGNNNCTG 1 cut(s) 1244
Ama87I CYCGRG 1 cut(s) 647
AoxI GGCC 2 cut(s) 1179, 1621
ApeKI GCWGC 2 cut(s) 311, 817
ApoI RAATTY 2 cut(s) 902, 1338
Asp700I GAANNNNTTC 2 cut(s) 996, 1076
AsuHPI GGTGA 4 cut(s) 350, 356, 544, 1114
AsuII TTCGAA 1 cut(s) 218
AvaI CYCGRG 1 cut(s) 647
BaeGI GKGCMC 2 cut(s) 664, 1254
BanI GGYRCC 1 cut(s) 1681
BanII GRGCYC 3 cut(s) 771, 882, 1129
BbsI GAAGAC 1 cut(s) 129
Bbv12I GWGCWC 2 cut(s) 771, 1129
BbvI GCAGC 2 cut(s) 298, 804
BccI CCATC 7 cut(s) 329, 335, 686, 1002, 1022, 1093, 1613
BciT130I CCWGG 1 cut(s) 477
BclI TGATCA 1 cut(s) 49
BcoDI GTCTC 4 cut(s) 622, 650, 1032, 1284
BcuI ACTAGT 1 cut(s) 172
BfaI CTAG 4 cut(s) 173, 533, 623, 800
BfmI CTRYAG 1 cut(s) 1236
BglII AGATCT 1 cut(s) 981
BisI GCNGC 3 cut(s) 251, 312, 818
BlpI GCTNAGC 1 cut(s) 485
BlsI GCNGC 3 cut(s) 252, 313, 819
Bme1390I CCNGG 1 cut(s) 477
BmeT110I CYCGRG 1 cut(s) 647
BmiI GGNNCC 2 cut(s) 1190, 1683
BmrFI CCNGG 1 cut(s) 477
BmsI GCATC 7 cut(s) 32, 229, 514, 1190, 1212, 1250, 1471
BpiI GAAGAC 1 cut(s) 129
BpmI CTGGAG 1 cut(s) 587
Bpu10I CCTNAGC 1 cut(s) 42
Bpu1102I GCTNAGC 1 cut(s) 485
Bpu14I TTCGAA 1 cut(s) 218
BpuEI CTTGAG 2 cut(s) 600, 998
BsaAI YACGTR 1 cut(s) 1571
BsaBI GATNNNNATC 1 cut(s) 738
BsaI GGTCTC 1 cut(s) 622
BsaJI CCNNGG 2 cut(s) 364, 1302
BsaXI ACNNNNNCTCC 2 cut(s) 1281, 1311
Bsc4I CCNNNNNNNGG 2 cut(s) 1213, 1317
Bse1I ACTGG 3 cut(s) 201, 300, 603
Bse8I GATNNNNATC 1 cut(s) 738
BseBI CCWGG 1 cut(s) 477
BseDI CCNNGG 2 cut(s) 364, 1302
BseGI GGATG 5 cut(s) 946, 1033, 1203, 1205, 1381
BseJI GATNNNNATC 1 cut(s) 738
BseLI CCNNNNNNNGG 2 cut(s) 1213, 1317
BseMII CTCAG 3 cut(s) 500, 784, 1549
BseNI ACTGG 3 cut(s) 201, 300, 603
BseRI GAGGAG 4 cut(s) 196, 1106, 1109, 1541
BseSI GKGCMC 2 cut(s) 664, 1254
BseXI GCAGC 2 cut(s) 298, 804
BseYI CCCAGC 3 cut(s) 855, 1208, 1247
BshFI GGCC 2 cut(s) 1181, 1623
BshNI GGYRCC 1 cut(s) 1681
BsiHKAI GWGCWC 2 cut(s) 771, 1129
BsiHKCI CYCGRG 1 cut(s) 647
BsiSI CCGG 1 cut(s) 1679
BslI CCNNNNNNNGG 2 cut(s) 1213, 1317
BsmAI GTCTC 4 cut(s) 622, 650, 1032, 1284
BsmI GAATGC 2 cut(s) 486, 956
BsnI GGCC 2 cut(s) 1181, 1623
Bso31I GGTCTC 1 cut(s) 622
BsoBI CYCGRG 1 cut(s) 647
Bsp119I TTCGAA 1 cut(s) 218
Bsp1286I GDGCHC 5 cut(s) 664, 771, 882, 1129, 1254
Bsp143I GATC 5 cut(s) 49, 97, 981, 1597, 1675
Bsp1720I GCTNAGC 1 cut(s) 485
BspACI CCGC 4 cut(s) 250, 268, 632, 1595
BspANI GGCC 2 cut(s) 1181, 1623
BspCNI CTCAG 3 cut(s) 501, 783, 1548
BspHI TCATGA 1 cut(s) 1644
BspLI GGNNCC 2 cut(s) 1190, 1683
BspPI GGATC 3 cut(s) 105, 1605, 1670
BspQI GCTCTTC 1 cut(s) 1108
BspT104I TTCGAA 1 cut(s) 218
BspT107I GGYRCC 1 cut(s) 1681
BspTNI GGTCTC 1 cut(s) 622
BsrBI CCGCTC 1 cut(s) 634
BsrI ACTGG 3 cut(s) 201, 300, 603
BssECI CCNNGG 2 cut(s) 364, 1302
BssMI GATC 5 cut(s) 49, 97, 981, 1597, 1675
BssT1I CCWWGG 2 cut(s) 364, 1302
Bst2UI CCWGG 1 cut(s) 477
Bst6I CTCTTC 2 cut(s) 531, 1108
BstBAI YACGTR 1 cut(s) 1571
BstBI TTCGAA 1 cut(s) 218
BstC8I GCNNGC 1 cut(s) 1014
BstDEI CTNAG 7 cut(s) 42, 380, 485, 509, 770, 1343, 1535
BstEII GGTNACC 1 cut(s) 344
BstF5I GGATG 5 cut(s) 946, 1033, 1203, 1205, 1381
BstKTI GATC 5 cut(s) 52, 100, 984, 1600, 1678
BstMAI GTCTC 4 cut(s) 622, 650, 1032, 1284
BstMBI GATC 5 cut(s) 49, 97, 981, 1597, 1675
BstMWI GCNNNNNNNGC 6 cut(s) 20, 481, 1124, 1133, 1142, 1209
BstNI CCWGG 1 cut(s) 477
BstNSI RCATGY 4 cut(s) 1016, 1048, 1418, 1634
BstPI GGTNACC 1 cut(s) 344
BstSCI CCNGG 1 cut(s) 475
BstSFI CTRYAG 1 cut(s) 1236
BstSLI GKGCMC 2 cut(s) 664, 1254
BstV1I GCAGC 2 cut(s) 298, 804
BstV2I GAAGAC 1 cut(s) 129
BstX2I RGATCY 2 cut(s) 981, 1597
BstXI CCANNNNNNTGG 1 cut(s) 1024
BstYI RGATCY 2 cut(s) 981, 1597
BsuRI GGCC 2 cut(s) 1181, 1623
BtsCI GGATG 5 cut(s) 946, 1033, 1203, 1205, 1381
BtsIMutI CAGTG 3 cut(s) 208, 293, 778
Cac8I GCNNGC 1 cut(s) 1014
CaiI CAGNNNCTG 1 cut(s) 1244
CciI TCATGA 1 cut(s) 1644
CseI GACGC 1 cut(s) 962
Csp6I GTAC 3 cut(s) 265, 963, 1568
CviQI GTAC 3 cut(s) 265, 963, 1568
DdeI CTNAG 7 cut(s) 42, 380, 485, 509, 770, 1343, 1535
DpnI GATC 5 cut(s) 51, 99, 983, 1599, 1677
DpnII GATC 5 cut(s) 49, 97, 981, 1597, 1675
Eam1104I CTCTTC 2 cut(s) 531, 1108
EarI CTCTTC 2 cut(s) 531, 1108
EciI GGCGGA 1 cut(s) 1610
Ecl136II GAGCTC 2 cut(s) 769, 1127
Eco130I CCWWGG 2 cut(s) 364, 1302
Eco24I GRGCYC 3 cut(s) 771, 882, 1129
Eco31I GGTCTC 1 cut(s) 622
Eco32I GATATC 2 cut(s) 946, 1519
Eco53kI GAGCTC 2 cut(s) 769, 1127
Eco57I CTGAAG 1 cut(s) 141
Eco88I CYCGRG 1 cut(s) 647
Eco91I GGTNACC 1 cut(s) 344
EcoICRI GAGCTC 2 cut(s) 769, 1127
EcoO65I GGTNACC 1 cut(s) 344
EcoRII CCWGG 1 cut(s) 475
EcoRV GATATC 2 cut(s) 946, 1519
EcoT14I CCWWGG 2 cut(s) 364, 1302
EcoT22I ATGCAT 1 cut(s) 1205
EcoT38I GRGCYC 3 cut(s) 771, 882, 1129
ErhI CCWWGG 2 cut(s) 364, 1302
FalI AAGNNNNNCTT 4 cut(s) 443, 475, 1295, 1327
FbaI TGATCA 1 cut(s) 49
Fnu4HI GCNGC 3 cut(s) 251, 312, 818
FokI GGATG 5 cut(s) 953, 1040, 1190, 1212, 1388
FriOI GRGCYC 3 cut(s) 771, 882, 1129
Fsp4HI GCNGC 3 cut(s) 251, 312, 818
FspBI CTAG 4 cut(s) 173, 533, 623, 800
GluI GCNGC 3 cut(s) 251, 312, 818
GsaI CCCAGC 3 cut(s) 859, 1212, 1251
GsuI CTGGAG 1 cut(s) 587
HaeIII GGCC 2 cut(s) 1181, 1623
HapII CCGG 1 cut(s) 1679
HgaI GACGC 1 cut(s) 962
HindIII AAGCTT 5 cut(s) 457, 487, 693, 1143, 1164
HinfI GANTC 6 cut(s) 116, 220, 569, 643, 1307, 1505
HpaII CCGG 1 cut(s) 1679
HphI GGTGA 4 cut(s) 350, 356, 544, 1114
Hpy166II GTNNAC 1 cut(s) 195
Hpy188I TCNGA 7 cut(s) 121, 225, 510, 638, 733, 1462, 1538
Hpy188III TCNNGA 4 cut(s) 566, 647, 649, 1645
Hpy8I GTNNAC 1 cut(s) 195
Hpy99I CGWCG 1 cut(s) 66
HpyAV CCTTC 5 cut(s) 804, 1099, 1103, 1190, 1495
HpyCH4IV ACGT 1 cut(s) 1570
HpyF10VI GCNNNNNNNGC 6 cut(s) 20, 481, 1124, 1133, 1142, 1209
HpyF3I CTNAG 7 cut(s) 42, 380, 485, 509, 770, 1343, 1535
HpySE526I ACGT 1 cut(s) 1570
Ksp22I TGATCA 1 cut(s) 49
Kzo9I GATC 5 cut(s) 49, 97, 981, 1597, 1675
LguI GCTCTTC 1 cut(s) 1108
LmnI GCTCC 2 cut(s) 639, 766
Lsp1109I GCAGC 2 cut(s) 298, 804
LweI GCATC 7 cut(s) 32, 229, 514, 1190, 1212, 1250, 1471
MaeI CTAG 4 cut(s) 173, 533, 623, 800
MaeII ACGT 1 cut(s) 1570
MaeIII GTNAC 3 cut(s) 344, 1297, 1436
MalI GATC 5 cut(s) 51, 99, 983, 1599, 1677
MbiI CCGCTC 1 cut(s) 634
MboI GATC 5 cut(s) 49, 97, 981, 1597, 1675
MboII GAAGA 9 cut(s) 134, 164, 299, 536, 548, 909, 983, 1048, 1125
MfeI CAATTG 1 cut(s) 1018
MflI RGATCY 2 cut(s) 981, 1597
MhlI GDGCHC 5 cut(s) 664, 771, 882, 1129, 1254
MlyI GAGTC 3 cut(s) 578, 652, 1316
MmeI TCCRAC 1 cut(s) 1615
Mph1103I ATGCAT 1 cut(s) 1205
MroXI GAANNNNTTC 2 cut(s) 996, 1076
MseI TTAA 4 cut(s) 461, 716, 1578, 1583
MslI CAYNNNNRTG 4 cut(s) 306, 324, 330, 1687
MspA1I CMGCKG 1 cut(s) 250
MspI CCGG 1 cut(s) 1679
MspR9I CCNGG 1 cut(s) 477
MunI CAATTG 1 cut(s) 1018
Mva1269I GAATGC 2 cut(s) 486, 956
MvaI CCWGG 1 cut(s) 477
MwoI GCNNNNNNNGC 6 cut(s) 20, 481, 1124, 1133, 1142, 1209
NdeII GATC 5 cut(s) 49, 97, 981, 1597, 1675
NlaIV GGNNCC 2 cut(s) 1190, 1683
NmeAIII GCCGAG 1 cut(s) 234
NmuCI GTSAC 1 cut(s) 344
NsiI ATGCAT 1 cut(s) 1205
NspI RCATGY 4 cut(s) 1016, 1048, 1418, 1634
NspV TTCGAA 1 cut(s) 218
PaeI GCATGC 1 cut(s) 1016
PaeR7I CTCGAG 1 cut(s) 647
PagI TCATGA 1 cut(s) 1644
PciI ACATGT 1 cut(s) 1630
PciSI GCTCTTC 1 cut(s) 1108
PctI GAATGC 2 cut(s) 486, 956
PdmI GAANNNNTTC 2 cut(s) 996, 1076
PfeI GAWTC 3 cut(s) 116, 220, 1505
PkrI GCNGC 3 cut(s) 252, 313, 819
PleI GAGTC 3 cut(s) 577, 651, 1315
PpsI GAGTC 3 cut(s) 577, 651, 1315
Ppu21I YACGTR 1 cut(s) 1571
PscI ACATGT 1 cut(s) 1630
PsiI TTATAA 1 cut(s) 131
Psp124BI GAGCTC 2 cut(s) 771, 1129
Psp6I CCWGG 1 cut(s) 475
PspEI GGTNACC 1 cut(s) 344
PspFI CCCAGC 3 cut(s) 855, 1208, 1247
PspGI CCWGG 1 cut(s) 475
PspN4I GGNNCC 2 cut(s) 1190, 1683
PstNI CAGNNNCTG 1 cut(s) 1244
PsuI RGATCY 2 cut(s) 981, 1597
RsaI GTAC 3 cut(s) 266, 964, 1569
RsaNI GTAC 3 cut(s) 265, 963, 1568
RseI CAYNNNNRTG 4 cut(s) 306, 324, 330, 1687
SacI GAGCTC 2 cut(s) 771, 1129
SapI GCTCTTC 1 cut(s) 1108
SaqAI TTAA 4 cut(s) 461, 716, 1578, 1583
SatI GCNGC 3 cut(s) 251, 312, 818
Sau3AI GATC 5 cut(s) 49, 97, 981, 1597, 1675
SchI GAGTC 3 cut(s) 578, 652, 1316
ScrFI CCNGG 1 cut(s) 477
SduI GDGCHC 5 cut(s) 664, 771, 882, 1129, 1254
SfaNI GCATC 7 cut(s) 32, 229, 514, 1190, 1212, 1250, 1471
SfcI CTRYAG 1 cut(s) 1236
Sfr274I CTCGAG 1 cut(s) 647
SfuI TTCGAA 1 cut(s) 218
SlaI CTCGAG 1 cut(s) 647
SmiMI CAYNNNNRTG 4 cut(s) 306, 324, 330, 1687
SmlI CTYRAG 3 cut(s) 615, 647, 977
SmoI CTYRAG 3 cut(s) 615, 647, 977
SpeI ACTAGT 1 cut(s) 172
SphI GCATGC 1 cut(s) 1016
SsiI CCGC 4 cut(s) 250, 268, 632, 1595
SspI AATATT 2 cut(s) 414, 1053
SspMI CTAG 4 cut(s) 173, 533, 623, 800
SstI GAGCTC 2 cut(s) 771, 1129
StyD4I CCNGG 1 cut(s) 475
StyI CCWWGG 2 cut(s) 364, 1302
TaiI ACGT 1 cut(s) 1573
TaqI TCGA 5 cut(s) 5, 61, 218, 648, 1388
TauI GCSGC 1 cut(s) 253
TfiI GAWTC 3 cut(s) 116, 220, 1505
Tru1I TTAA 4 cut(s) 461, 716, 1578, 1583
Tru9I TTAA 4 cut(s) 461, 716, 1578, 1583
TscAI CASTG 3 cut(s) 208, 300, 778
TseFI GTSAC 1 cut(s) 344
TseI GCWGC 2 cut(s) 311, 817
Tsp45I GTSAC 1 cut(s) 344
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 3 cut(s) 208, 300, 778
XapI RAATTY 2 cut(s) 902, 1338
XceI RCATGY 4 cut(s) 1016, 1048, 1418, 1634
XcmI CCANNNNNNNNNTGG 3 cut(s) 332, 562, 890
XhoI CTCGAG 1 cut(s) 647
XmnI GAANNNNTTC 2 cut(s) 996, 1076
XspI CTAG 4 cut(s) 173, 533, 623, 800
Zsp2I ATGCAT 1 cut(s) 1205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.