Rorug03G0296000

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000053
Physical Location & Seq
Forward (+)
30488175 .. 30492161
3987 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0296000.1

Sequence Viewer

Length: 2634 bp
ATGGCGTCAACGCCGCCGCACTGTTCAATCACAGCGACCAAGCCGTACCAAACCCACCAATACCCACAAAACCAGCGCCTCAAATCTCACCGCCAGACCCGTCAGAGCAACCAATGGACCACCCACCACGTCTCTCTCTCAAAGCCTCTACCTTTGCCTCCCCAACCGCCGCGCACGGCGCCCAAACCCGCCGCCGCCGCCGCCGCCAACGCTCCCAGTCCGACCTCCTTTTCCTCTCTCTGCCCTCCGCCCAAGTCCGACCTCGTCGCCGCCTTCTCGGGCCGCCGCTCGACCCGGTTCGTGTCCAAAATGCACTTCGGCCGGCCCAAAACCACGGTGGGGTCCCGTCACTCCTCGCTGGCGGAGGAGGCCCTGCAGAGCGCAATCCAGTTCGGTAAGGACGATTTGGCCCTTGATGATGTTTTGCTTAGTTTCGAGTCTAGGCTTTGTGGGTCGGATGATTACACTTTTTTGCTTAGAGAGCTTGGGAATAGAGGTGAGTGTTGGAAGGCTATAAGGTGCTTCGAGTTTGCGGTGAGGAGGGAGAGAAGGAGAACTGAACAGGGGAAATTAGCTAGCTCTATGATTAGTACTCTTGGTAGGTTGGGGAAGGTTGAGCTTGCCAAGAATGTGTTTCAAACTGCTGTCAATGAGGGTTATGGCAAGACTGTTTATACGTATTCGGCTTTGATTAGTGCTTATGGGCGCAGTGGGTATTGCGATGAGGCTATTCGAGTGCTCGAGTCCATGAAAGATTCGGGCTTGAAGCCGAATTTGGTTACTTACAATGCGGTGATTGATGCATGTGGAAAAGGGGGAGTGGAGTTTAAGAAGGTAGTGGAGATTTTCAATGAGATGCTTAAGATTGGGGTGCAGCCTGATCGAATTACTTATAATTCTCTCCTTGCGGTTTGTAGTCGAGGGGGACTGTGGGAGGCGGCTAGAAACTTGTTCAGTGAGATGGTGGATAGGGGGATTGATCAGGATATTTATACCTATAATACGCTTTTGGATGCAATTTCTAAAGGTGGGCAGATGGATTTGGCATATCAGATTATGTCAGAGATGCCTTCAAAGAATATTTTGCCTAATGTGGTGACTTATAGTACTATGATCGATGGTTATGCGAAGGCTGGTAGATTAGAAGATGCACTCAGTTTATTTAATGAAATGAAGTTTTTGGCCATTGGTCTGGATAGAGTTCTGTATAATACATTGCTGTCACTTTACGGGAAGCTTGGCAGGTTTGCAGAGGCTTTGAATGTTTGCAAGGAGATGGAGAGTGTTGGGATTGTGAAGGACGTTGTATCTTATAATGCACTTCTTGGTGGGTATGGGAAGCAAGGGAAGTATGATGAAGTTAAAGGACTGTACAGTGAAATGAAAGAAGAACGTGTATCACCAAATTTATTAACTTATTCAACATTGATCGATGTGTATTCAAAAGGTGGTTTGTATGTGGAGGCAATGAAAGTTTTCAGAGAGTTTAAGCAGGCAGGGCTGAAGGCTGATGTTGTGCTCTATAGTGAACTTATTAATGCTTTGTGCAAAAATGGGCTGGTGGAATCTGCTGTTTCATTGCTTGATGAGATGACCAAGGAAGGCATCCGACCTAATGTTGTCACTTATAATTGTATAATTGATGCCTTTGGTAGGCCAGCGACAACAGAGTGTGCAGTTGGTACTGGTGCTGGAGGCATTGTGCTACACAATGAATCTCCATCTTCAATTTCTGAAAGGGATGTTGACATTTCTGAAGAGAATGTTCAAATTCAGGTGAGAGATATGGAGGATACCAGAATTATAAAGATGTTCGGGCAGCTTGCAGCTGATAAAGCTGGTTATGCAAAGAAAGATAGGAAGGTTAGACAAGAAATCTTGTGCATTTTGGGTGTTGTTCAGAAGATGCATGAGCTAGACATCAAACCGAATGTTGTCACATTTTCAGCCATTCTAAATGCTTGCAGCCGCTGTAATTCATTTGAAGATGCTTCCATGTTATTGGAGGAATTGCGGTTGTTTGATAACCAGGTTTATGGTGTTGCCCATGGACTTCTTATGGGCTACAGGGGTAATGTATGGGTGAAAGCTCAGTCCCTGTTTGATGAAGTTAAGCAGATGGACTGTTCAACTGCATCTGCATTCTACAATGCTCTTACCGACATGCTATGGCACTTTGGACAGAAACAAGGGGCGCAACTGGTTGTGCTTGAAGGGAAGCGCCGAAATGTATGGGAGAATGCATGGGAGAATTCACAGTTAGATTTGCATTTGATGTCTTCTGGGGCTGCCCGAGCAATGGTTCATGCATGGTTGCTCAATATACACTCTATAGTGTATAAAGGCCAACAATTGCCAAACCTATTAAGCATTTTGACAGGATGGGGCAAGCACAGCAAAGTAGTAGGTGACAGTGCCTTAAGACGAGCAATCGAGGCACTTCTTACCAGCATGGGTGCACCCTTTCAGGTTGCCAAGCCTAACATAGGTAGGTTTATTTCAACGGGATCTGTGGCTGCTGCATGGTTAAAAGAATCTGGCACACTGGAGGTGCTCAAGCTTCATGATGATAGGGCTGATCCAAATAGTGCAAATTTTGGCCAAATATCCAATTTACGAGCGCTCACTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

877

Amino Acids

96.9

Weight (kDa)

8.55

Isoelectric Point (pI)

38.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 195 - 287 4.1e-09 Fungal tetratrico peptide repeats
PPR_3 PF13812 211 - 271 6.9e-13 Pentatricopeptide repeat domain
PPR_2 PF13041 223 - 271 3.4e-13 PPR repeat family
PPR_1 PF12854 223 - 251 4e-06 PPR repeat
PPR PF01535 225 - 255 7.3e-08 PPR repeat
PPR_2 PF13041 296 - 341 1.4e-11 PPR repeat family
PPR PF01535 296 - 326 1.4e-06 PPR repeat
PPR_long PF17177 303 - 452 6.6e-10 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 317 - 375 1.4e-12 Pentatricopeptide repeat domain
PPR_2 PF13041 329 - 377 5.1e-16 PPR repeat family
PPR_3 PF13812 352 - 412 1.7e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 359 - 392 1.2e-13 PPR repeat
PPR_2 PF13041 363 - 393 2.4e-10 PPR repeat family
PPR PF01535 366 - 392 4.8e-10 PPR repeat
PPR PF01535 401 - 431 9.2e-06 PPR repeat
PPR_3 PF13812 422 - 481 1.8e-12 Pentatricopeptide repeat domain
PPR_2 PF13041 433 - 481 3.4e-15 PPR repeat family
PPR PF01535 436 - 465 1.1e-07 PPR repeat
PPR PF01535 472 - 501 3.3e-06 PPR repeat
TPR_24 PF23276 488 - 551 8.7e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 491 - 551 2.1e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 500 - 532 6.2e-10 PPR repeat
PPR_2 PF13041 504 - 551 6.6e-16 PPR repeat family
PPR PF01535 506 - 536 5.8e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 894, 1314, 1629, 1805
Acc36I ACCTGC 1 cut(s) 1233
AccB1I GGYRCC 1 cut(s) 178
AccBSI CCGCTC 1 cut(s) 288
AccII CGCG 1 cut(s) 172
AclWI GGATC 2 cut(s) 2515, 2573
AcoI YGGCCR 3 cut(s) 319, 1182, 2599
AcsI RAATTY 5 cut(s) 772, 1405, 1770, 2251, 2593
AcuI CTGAAG 2 cut(s) 1523, 1776
AcyI GRCGYC 2 cut(s) 5, 179
AfaI GTAC 5 cut(s) 47, 592, 1108, 1373, 1684
AfeI AGCGCT 1 cut(s) 2622
AfiI CCNNNNNNNGG 4 cut(s) 339, 1653, 2299, 2486
AflII CTTAAG 2 cut(s) 860, 2419
AflIII ACRYGT 1 cut(s) 1393
AjiI CACGTC 1 cut(s) 130
AjnI CCWGG 1 cut(s) 2028
AjuI GAANNNNNNNTTGG 4 cut(s) 299, 331, 758, 790
Alw21I GWGCWC 4 cut(s) 741, 1521, 2461, 2556
Alw26I GTCTC 1 cut(s) 136
Alw44I GTGCAC 1 cut(s) 2457
AlwI GGATC 2 cut(s) 2515, 2573
AlwNI CAGNNNCTG 2 cut(s) 1971, 2098
Ama87I CYCGRG 3 cut(s) 277, 740, 2292
Aor51HI AGCGCT 1 cut(s) 2622
AoxI GGCC 9 cut(s) 280, 319, 323, 369, 408, 1182, 1655, 2344, 2599
ApaLI GTGCAC 1 cut(s) 2457
ApeKI GCWGC 7 cut(s) 874, 1819, 1826, 1965, 2288, 2516, 2519
ApoI RAATTY 5 cut(s) 772, 1405, 1770, 2251, 2593
AseI ATTAAT 1 cut(s) 1536
Asp700I GAANNNNTTC 1 cut(s) 1475
AspLEI GCGC 8 cut(s) 78, 174, 181, 383, 708, 2197, 2223, 2623
AspS9I GGNCC 6 cut(s) 117, 280, 324, 342, 370, 409
AsuC2I CCSGG 1 cut(s) 295
AsuHPI GGTGA 9 cut(s) 80, 509, 547, 805, 1108, 1392, 1789, 2095, 2420
AsuNHI GCTAGC 1 cut(s) 575
AvaI CYCGRG 3 cut(s) 277, 740, 2292
AvaII GGWCC 2 cut(s) 117, 342
BaeGI GKGCMC 1 cut(s) 2461
BalI TGGCCA 2 cut(s) 1184, 2601
BanI GGYRCC 1 cut(s) 178
BbsI GAAGAC 1 cut(s) 2271
Bbv12I GWGCWC 4 cut(s) 741, 1521, 2461, 2556
BbvI GCAGC 7 cut(s) 886, 1831, 1838, 1977, 2275, 2503, 2506
BccI CCATC 7 cut(s) 955, 1030, 1112, 1270, 1729, 2113, 2376
BceAI ACGGC 2 cut(s) 28, 192
BcgI CGANNNNNNTGC 4 cut(s) 863, 897, 1106, 1140
BciT130I CCWGG 1 cut(s) 2030
BciVI GTATCC 1 cut(s) 1786
BclI TGATCA 1 cut(s) 979
BcnI CCSGG 1 cut(s) 295
BcoDI GTCTC 1 cut(s) 136
BfaI CTAG 4 cut(s) 441, 576, 942, 1916
BfmI CTRYAG 4 cut(s) 374, 1522, 2065, 2331
BfoI RGCGCY 4 cut(s) 79, 182, 2224, 2624
BfrI CTTAAG 2 cut(s) 860, 2419
BfuAI ACCTGC 1 cut(s) 1233
BfuI GTATCC 1 cut(s) 1786
BmcAI AGTACT 2 cut(s) 592, 1108
Bme1390I CCNGG 2 cut(s) 295, 2030
Bme18I GGWCC 2 cut(s) 117, 342
BmeT110I CYCGRG 3 cut(s) 277, 740, 2292
BmgBI CACGTC 1 cut(s) 130
BmgT120I GGNCC 6 cut(s) 117, 280, 324, 342, 370, 409
BmiI GGNNCC 3 cut(s) 180, 343, 344
BmrFI CCNGG 2 cut(s) 295, 2030
BmrI ACTGGG 1 cut(s) 210
BmtI GCTAGC 1 cut(s) 579
BmuI ACTGGG 1 cut(s) 210
BpiI GAAGAC 1 cut(s) 2271
BpmI CTGGAG 2 cut(s) 1713, 2567
BpuEI CTTGAG 1 cut(s) 2540
BpuMI CCSGG 1 cut(s) 295
Bsa29I ATCGAT 2 cut(s) 1116, 1431
BsaAI YACGTR 1 cut(s) 678
BsaHI GRCGYC 2 cut(s) 5, 179
BsaJI CCNNGG 3 cut(s) 333, 1596, 2047
BsaXI ACNNNNNCTCC 2 cut(s) 1686, 1716
Bsc4I CCNNNNNNNGG 4 cut(s) 339, 1653, 2299, 2486
Bse118I RCCGGY 1 cut(s) 321
Bse1I ACTGG 5 cut(s) 216, 388, 1690, 2205, 2550
Bse3DI GCAATG 4 cut(s) 1214, 1473, 1577, 2304
BseBI CCWGG 1 cut(s) 2030
BseCI ATCGAT 2 cut(s) 1116, 1431
BseDI CCNNGG 3 cut(s) 333, 1596, 2047
BseGI GGATG 5 cut(s) 463, 1018, 1605, 1747, 2387
BseLI CCNNNNNNNGG 4 cut(s) 339, 1653, 2299, 2486
BseMI GCAATG 4 cut(s) 1214, 1473, 1577, 2304
BseMII CTCAG 2 cut(s) 1168, 2105
BseNI ACTGG 5 cut(s) 216, 388, 1690, 2205, 2550
BseRI GAGGAG 3 cut(s) 343, 380, 553
BseSI GKGCMC 1 cut(s) 2461
BseX3I CGGCCG 1 cut(s) 319
BseXI GCAGC 7 cut(s) 886, 1831, 1838, 1977, 2275, 2503, 2506
BsgI GTGCAG 2 cut(s) 893, 1695
Bsh1236I CGCG 1 cut(s) 172
Bsh1285I CGRYCG 1 cut(s) 322
BshFI GGCC 9 cut(s) 282, 321, 325, 371, 410, 1184, 1657, 2346, 2601
BshNI GGYRCC 1 cut(s) 178
BshVI ATCGAT 2 cut(s) 1116, 1431
BsiEI CGRYCG 1 cut(s) 322
BsiHKAI GWGCWC 4 cut(s) 741, 1521, 2461, 2556
BsiHKCI CYCGRG 3 cut(s) 277, 740, 2292
BsiSI CCGG 2 cut(s) 295, 322
BslFI GGGAC 3 cut(s) 328, 939, 2080
BslI CCNNNNNNNGG 4 cut(s) 339, 1653, 2299, 2486
BsmAI GTCTC 1 cut(s) 136
BsmBI CGTCTC 1 cut(s) 136
BsmFI GGGAC 3 cut(s) 328, 939, 2080
BsmI GAATGC 2 cut(s) 2141, 2245
BsnI GGCC 9 cut(s) 282, 321, 325, 371, 410, 1184, 1657, 2346, 2601
BsoBI CYCGRG 3 cut(s) 277, 740, 2292
Bsp1286I GDGCHC 4 cut(s) 741, 1521, 2461, 2556
Bsp1407I TGTACA 1 cut(s) 1371
Bsp143I GATC 6 cut(s) 880, 979, 1113, 1428, 2507, 2578
Bsp19I CCATGG 1 cut(s) 2047
BspANI GGCC 9 cut(s) 282, 321, 325, 371, 410, 1184, 1657, 2346, 2601
BspCNI CTCAG 2 cut(s) 1167, 2104
BspDI ATCGAT 2 cut(s) 1116, 1431
BspFNI CGCG 1 cut(s) 172
BspHI TCATGA 1 cut(s) 2563
BspLI GGNNCC 3 cut(s) 180, 343, 344
BspMAI CTGCAG 1 cut(s) 378
BspMI ACCTGC 1 cut(s) 1233
BspOI GCTAGC 1 cut(s) 579
BspPI GGATC 2 cut(s) 2515, 2573
BspT107I GGYRCC 1 cut(s) 178
BspTI CTTAAG 2 cut(s) 860, 2419
BsrBI CCGCTC 1 cut(s) 288
BsrDI GCAATG 4 cut(s) 1214, 1473, 1577, 2304
BsrFI RCCGGY 1 cut(s) 321
BsrGI TGTACA 1 cut(s) 1371
BsrI ACTGG 5 cut(s) 216, 388, 1690, 2205, 2550
BssAI RCCGGY 1 cut(s) 321
BssECI CCNNGG 3 cut(s) 333, 1596, 2047
BssMI GATC 6 cut(s) 880, 979, 1113, 1428, 2507, 2578
BssNI GRCGYC 2 cut(s) 5, 179
BssT1I CCWWGG 2 cut(s) 1596, 2047
Bst2UI CCWGG 1 cut(s) 2030
Bst4CI ACNGT 9 cut(s) 23, 337, 670, 930, 1371, 1376, 2126, 2259, 2414
Bst6I CTCTTC 1 cut(s) 1752
BstACI GRCGYC 2 cut(s) 5, 179
BstAFI CTTAAG 2 cut(s) 860, 2419
BstAUI TGTACA 1 cut(s) 1371
BstBAI YACGTR 1 cut(s) 678
BstC8I GCNNGC 9 cut(s) 323, 360, 577, 621, 1494, 1659, 1824, 1963, 2390
BstDEI CTNAG 4 cut(s) 428, 476, 1154, 2091
BstDSI CCRYGG 2 cut(s) 333, 2047
BstENI CCTNNNNNAGG 2 cut(s) 1651, 2484
BstF5I GGATG 5 cut(s) 463, 1018, 1605, 1747, 2387
BstFNI CGCG 1 cut(s) 172
BstH2I RGCGCY 4 cut(s) 79, 182, 2224, 2624
BstHHI GCGC 8 cut(s) 78, 174, 181, 383, 708, 2197, 2223, 2623
BstKTI GATC 6 cut(s) 883, 982, 1116, 1431, 2510, 2581
BstMAI GTCTC 1 cut(s) 136
BstMBI GATC 6 cut(s) 880, 979, 1113, 1428, 2507, 2578
BstMCI CGRYCG 1 cut(s) 322
BstNI CCWGG 1 cut(s) 2030
BstNSI RCATGY 2 cut(s) 807, 2167
BstSCI CCNGG 2 cut(s) 293, 2028
BstSFI CTRYAG 4 cut(s) 374, 1522, 2065, 2331
BstSLI GKGCMC 1 cut(s) 2461
BstSNI TACGTA 1 cut(s) 678
BstUI CGCG 1 cut(s) 172
BstV1I GCAGC 7 cut(s) 886, 1831, 1838, 1977, 2275, 2503, 2506
BstV2I GAAGAC 1 cut(s) 2271
BstX2I RGATCY 1 cut(s) 2507
BstXI CCANNNNNNTGG 3 cut(s) 1192, 2002, 2036
BstYI RGATCY 1 cut(s) 2507
BstZI CGGCCG 1 cut(s) 319
Bsu15I ATCGAT 2 cut(s) 1116, 1431
BsuI GTATCC 1 cut(s) 1786
BsuRI GGCC 9 cut(s) 282, 321, 325, 371, 410, 1184, 1657, 2346, 2601
BsuTUI ATCGAT 2 cut(s) 1116, 1431
BtgI CCRYGG 2 cut(s) 333, 2047
BtgZI GCGATG 1 cut(s) 735
BtrI CACGTC 1 cut(s) 130
BtsCI GGATG 5 cut(s) 463, 1018, 1605, 1747, 2387
BtsI GCAGTG 1 cut(s) 715
BtsIMutI CAGTG 6 cut(s) 19, 715, 961, 1381, 2419, 2543
BveI ACCTGC 1 cut(s) 1233
Cac8I GCNNGC 9 cut(s) 323, 360, 577, 621, 1494, 1659, 1824, 1963, 2390
CaiI CAGNNNCTG 2 cut(s) 1971, 2098
CciI TCATGA 1 cut(s) 2563
CfoI GCGC 8 cut(s) 78, 174, 181, 383, 708, 2197, 2223, 2623
Cfr10I RCCGGY 1 cut(s) 321
Cfr13I GGNCC 6 cut(s) 117, 280, 324, 342, 370, 409
ClaI ATCGAT 2 cut(s) 1116, 1431
CsiI ACCWGGT 1 cut(s) 2028
Csp6I GTAC 5 cut(s) 46, 591, 1107, 1372, 1683
CviQI GTAC 5 cut(s) 46, 591, 1107, 1372, 1683
DdeI CTNAG 4 cut(s) 428, 476, 1154, 2091
DinI GGCGCC 1 cut(s) 180
DpnI GATC 6 cut(s) 882, 981, 1115, 1430, 2509, 2580
DpnII GATC 6 cut(s) 880, 979, 1113, 1428, 2507, 2578
EaeI YGGCCR 3 cut(s) 319, 1182, 2599
EagI CGGCCG 1 cut(s) 319
Eam1104I CTCTTC 1 cut(s) 1752
EarI CTCTTC 1 cut(s) 1752
EciI GGCGGA 2 cut(s) 237, 377
EclXI CGGCCG 1 cut(s) 319
Eco105I TACGTA 1 cut(s) 678
Eco130I CCWWGG 2 cut(s) 1596, 2047
Eco47I GGWCC 2 cut(s) 117, 342
Eco47III AGCGCT 1 cut(s) 2622
Eco52I CGGCCG 1 cut(s) 319
Eco57I CTGAAG 2 cut(s) 1523, 1776
Eco88I CYCGRG 3 cut(s) 277, 740, 2292
EcoNI CCTNNNNNAGG 2 cut(s) 1651, 2484
EcoO109I RGGNCCY 2 cut(s) 342, 370
EcoRI GAATTC 1 cut(s) 2251
EcoRII CCWGG 1 cut(s) 2028
EcoT14I CCWWGG 2 cut(s) 1596, 2047
EcoT22I ATGCAT 4 cut(s) 805, 1911, 2245, 2311
EgeI GGCGCC 1 cut(s) 180
EheI GGCGCC 1 cut(s) 180
ErhI CCWWGG 2 cut(s) 1596, 2047
Esp3I CGTCTC 1 cut(s) 136
FaqI GGGAC 3 cut(s) 328, 939, 2080
FauI CCCGC 1 cut(s) 196
FbaI TGATCA 1 cut(s) 979
FokI GGATG 5 cut(s) 470, 1025, 1592, 1754, 2394
FseI GGCCGGCC 1 cut(s) 325
FspBI CTAG 4 cut(s) 441, 576, 942, 1916
GlaI GCGC 8 cut(s) 77, 173, 180, 382, 707, 2196, 2222, 2622
GsuI CTGGAG 2 cut(s) 1713, 2567
HaeII RGCGCY 4 cut(s) 79, 182, 2224, 2624
HaeIII GGCC 9 cut(s) 282, 321, 325, 371, 410, 1184, 1657, 2346, 2601
HapII CCGG 2 cut(s) 295, 322
HhaI GCGC 8 cut(s) 78, 174, 181, 383, 708, 2197, 2223, 2623
Hin1I GRCGYC 2 cut(s) 5, 179
Hin6I GCGC 8 cut(s) 76, 172, 179, 381, 706, 2195, 2221, 2621
HinP1I GCGC 8 cut(s) 76, 172, 179, 381, 706, 2195, 2221, 2621
HincII GTYRAC 2 cut(s) 9, 1747
HindII GTYRAC 2 cut(s) 9, 1747
HindIII AAGCTT 2 cut(s) 1235, 2558
HinfI GANTC 6 cut(s) 437, 743, 755, 1565, 1715, 2534
HpaII CCGG 2 cut(s) 295, 322
HphI GGTGA 9 cut(s) 80, 509, 547, 805, 1108, 1392, 1789, 2095, 2420
Hpy166II GTNNAC 4 cut(s) 9, 1529, 1747, 2459
Hpy188III TCNNGA 3 cut(s) 983, 1193, 2564
Hpy8I GTNNAC 4 cut(s) 9, 1529, 1747, 2459
Hpy99I CGWCG 1 cut(s) 269
HpyCH4III ACNGT 9 cut(s) 23, 337, 670, 930, 1371, 1376, 2126, 2259, 2414
HpyCH4IV ACGT 4 cut(s) 129, 677, 1302, 1393
HpyF3I CTNAG 4 cut(s) 428, 476, 1154, 2091
HpySE526I ACGT 4 cut(s) 129, 677, 1302, 1393
Hsp92I GRCGYC 2 cut(s) 5, 179
HspAI GCGC 8 cut(s) 76, 172, 179, 381, 706, 2195, 2221, 2621
KasI GGCGCC 1 cut(s) 178
KflI GGGWCCC 1 cut(s) 342
KroI GCCGGC 1 cut(s) 321
KroNI GCCGGC 1 cut(s) 323
Ksp22I TGATCA 1 cut(s) 979
Kzo9I GATC 6 cut(s) 880, 979, 1113, 1428, 2507, 2578
LmnI GCTCC 1 cut(s) 217
Lsp1109I GCAGC 7 cut(s) 886, 1831, 1838, 1977, 2275, 2503, 2506
MabI ACCWGGT 1 cut(s) 2028
MaeI CTAG 4 cut(s) 441, 576, 942, 1916
MaeII ACGT 4 cut(s) 129, 677, 1302, 1393
MaeIII GTNAC 7 cut(s) 347, 778, 1096, 1221, 1621, 1936, 2408
MalI GATC 6 cut(s) 882, 981, 1115, 1430, 2509, 2580
MbiI CCGCTC 1 cut(s) 288
MboI GATC 6 cut(s) 880, 979, 1113, 1428, 2507, 2578
MboII GAAGA 7 cut(s) 1157, 1400, 1716, 1769, 1915, 1997, 2271
MfeI CAATTG 1 cut(s) 2351
MflI RGATCY 1 cut(s) 2507
MhlI GDGCHC 4 cut(s) 741, 1521, 2461, 2556
MlsI TGGCCA 2 cut(s) 1184, 2601
MluNI TGGCCA 2 cut(s) 1184, 2601
Mly113I GGCGCC 1 cut(s) 179
MlyI GAGTC 2 cut(s) 446, 752
MmeI TCCRAC 5 cut(s) 245, 282, 435, 485, 1633
Mox20I TGGCCA 2 cut(s) 1184, 2601
Mph1103I ATGCAT 4 cut(s) 805, 1911, 2245, 2311
MroNI GCCGGC 1 cut(s) 321
MroXI GAANNNNTTC 1 cut(s) 1475
MscI TGGCCA 2 cut(s) 1184, 2601
Msp20I TGGCCA 2 cut(s) 1184, 2601
MspA1I CMGCKG 2 cut(s) 1829, 1971
MspCI CTTAAG 2 cut(s) 860, 2419
MspI CCGG 2 cut(s) 295, 322
MspR9I CCNGG 2 cut(s) 295, 2030
MunI CAATTG 1 cut(s) 2351
Mva1269I GAATGC 2 cut(s) 2141, 2245
MvaI CCWGG 1 cut(s) 2030
MvnI CGCG 1 cut(s) 172
NaeI GCCGGC 1 cut(s) 323
NarI GGCGCC 1 cut(s) 179
NciI CCSGG 1 cut(s) 295
NcoI CCATGG 1 cut(s) 2047
NdeII GATC 6 cut(s) 880, 979, 1113, 1428, 2507, 2578
NgoMIV GCCGGC 1 cut(s) 321
NheI GCTAGC 1 cut(s) 575
NlaIV GGNNCC 3 cut(s) 180, 343, 344
NmuCI GTSAC 6 cut(s) 347, 1096, 1221, 1621, 1936, 2408
NsiI ATGCAT 4 cut(s) 805, 1911, 2245, 2311
NspI RCATGY 2 cut(s) 807, 2167
PaeR7I CTCGAG 1 cut(s) 740
PagI TCATGA 1 cut(s) 2563
PcsI WCGNNNNNNNCGW 1 cut(s) 399
PctI GAATGC 2 cut(s) 2141, 2245
PdiI GCCGGC 1 cut(s) 323
PdmI GAANNNNTTC 1 cut(s) 1475
PfeI GAWTC 4 cut(s) 755, 1565, 1715, 2534
PflFI GACNNNGTC 1 cut(s) 263
PleI GAGTC 2 cut(s) 445, 751
PluTI GGCGCC 1 cut(s) 182
PpsI GAGTC 2 cut(s) 445, 751
Ppu21I YACGTR 1 cut(s) 678
PpuMI RGGWCCY 1 cut(s) 342
PshBI ATTAAT 1 cut(s) 1536
PsiI TTATAA 4 cut(s) 894, 1314, 1629, 1805
Psp5II RGGWCCY 1 cut(s) 342
Psp6I CCWGG 1 cut(s) 2028
PspGI CCWGG 1 cut(s) 2028
PspN4I GGNNCC 3 cut(s) 180, 343, 344
PspPI GGNCC 6 cut(s) 117, 280, 324, 342, 370, 409
PspPPI RGGWCCY 1 cut(s) 342
PspXI VCTCGAGB 1 cut(s) 740
PstI CTGCAG 1 cut(s) 378
PstNI CAGNNNCTG 2 cut(s) 1971, 2098
PsuI RGATCY 1 cut(s) 2507
PsyI GACNNNGTC 1 cut(s) 263
PvuII CAGCTG 1 cut(s) 1829
RigI GGCCGGCC 1 cut(s) 325
RsaI GTAC 5 cut(s) 47, 592, 1108, 1373, 1684
RsaNI GTAC 5 cut(s) 46, 591, 1107, 1372, 1683
Sau3AI GATC 6 cut(s) 880, 979, 1113, 1428, 2507, 2578
Sau96I GGNCC 6 cut(s) 117, 280, 324, 342, 370, 409
ScaI AGTACT 2 cut(s) 592, 1108
SchI GAGTC 2 cut(s) 446, 752
ScrFI CCNGG 2 cut(s) 295, 2030
SduI GDGCHC 4 cut(s) 741, 1521, 2461, 2556
SexAI ACCWGGT 1 cut(s) 2028
SfcI CTRYAG 4 cut(s) 374, 1522, 2065, 2331
SfoI GGCGCC 1 cut(s) 180
Sfr274I CTCGAG 1 cut(s) 740
SinI GGWCC 2 cut(s) 117, 342
SlaI CTCGAG 1 cut(s) 740
SmlI CTYRAG 4 cut(s) 740, 860, 2419, 2555
SmoI CTYRAG 4 cut(s) 740, 860, 2419, 2555
SnaBI TACGTA 1 cut(s) 678
SspDI GGCGCC 1 cut(s) 178
SspI AATATT 1 cut(s) 1081
SspMI CTAG 4 cut(s) 441, 576, 942, 1916
StyD4I CCNGG 2 cut(s) 293, 2028
StyI CCWWGG 2 cut(s) 1596, 2047
TaaI ACNGT 9 cut(s) 23, 337, 670, 930, 1371, 1376, 2126, 2259, 2414
TaiI ACGT 4 cut(s) 132, 680, 1305, 1396
TatI WGTACW 3 cut(s) 590, 1106, 1371
TfiI GAWTC 4 cut(s) 755, 1565, 1715, 2534
TscAI CASTG 6 cut(s) 26, 715, 961, 1381, 2419, 2550
TseFI GTSAC 6 cut(s) 347, 1096, 1221, 1621, 1936, 2408
TseI GCWGC 7 cut(s) 874, 1819, 1826, 1965, 2288, 2516, 2519
Tsp45I GTSAC 6 cut(s) 347, 1096, 1221, 1621, 1936, 2408
TspRI CASTG 6 cut(s) 26, 715, 961, 1381, 2419, 2550
Tth111I GACNNNGTC 1 cut(s) 263
Vha464I CTTAAG 2 cut(s) 860, 2419
VneI GTGCAC 1 cut(s) 2457
VpaK11BI GGWCC 2 cut(s) 117, 342
VspI ATTAAT 1 cut(s) 1536
XagI CCTNNNNNAGG 2 cut(s) 1651, 2484
XapI RAATTY 5 cut(s) 772, 1405, 1770, 2251, 2593
XceI RCATGY 2 cut(s) 807, 2167
XcmI CCANNNNNNNNNTGG 1 cut(s) 334
XhoI CTCGAG 1 cut(s) 740
XmnI GAANNNNTTC 1 cut(s) 1475
XspI CTAG 4 cut(s) 441, 576, 942, 1916
ZrmI AGTACT 2 cut(s) 592, 1108
Zsp2I ATGCAT 4 cut(s) 805, 1911, 2245, 2311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.