Rroxscaffold_3G00246840

(-)-germacrene D synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000053
Physical Location & Seq
Forward (+)
40162544 .. 40179306
16763 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00246840.1

Sequence Viewer

Length: 765 bp
ATGATGGCTATCCTCGATGACATTTATGATACCCATGGCACACTCGAAGAACTAGAGCTTTTTACTGAAGTTATTCACAGGTGGGATATCTGTGCCATAGATCCATTGCCAGATTATATGAAAGTCTGTTATAAGGCATTGTTAGAAGTCTATATTGAAATTGAAGAAGAGCTTGCAAAGGAGGGAAAGTTGTATCGAATCCACTATGCCGGAGAAGCTATGAAAAAACAAGCTGAAGTTTACTTCCTTGAAGCTAAATGGCTGCACCACCAACACATACCAACGATGGATGAATATATGGCCCTATCATTATTGACCTCAGGCTACCCTCAACTAATAACAACATCTTTTGTTGCAATTGGAGATATTGCTACAGACGACTCCTTCGATTGGTTGGCCACTTATCCCAGGGCAGTAAAAGGGGCAGCAGTAGTTGCTCGACTCATGGATGATATAGCGGACCACAAGTTTGAGGAAAAGAGAGGACATGTTATCTCAGCTGTGCAGTGCTACATGAATGAATGTGGTGTCACAGAAGAAGAAGCAATAACTGTATTGCGTAGACAAGTGAGTGACGCATGGAAGGACATCAACGAATCGTTCCTCCTTCCTAATGCTGTCCCGAGGCCACTACTAACCCGAATTCTTAATTTTGCATGTGCCATCGACGTTTCATACAAGTATGAAGATGGTTTCACTACTCATCATGAGACTGTGCTCAAGGATTTTATAATTTCTACGCTTGTCCAACCCATGCCTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

254

Amino Acids

29.13

Weight (kDa)

4.77

Isoelectric Point (pI)

52.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth_C PF03936 1 - 196 1.6e-73 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 2 - 196 1.4e-55 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 132, 731
AccI GTMKAC 1 cut(s) 562
AciI CCGC 1 cut(s) 458
AclWI GGATC 1 cut(s) 95
AcoI YGGCCR 1 cut(s) 396
AcsI RAATTY 1 cut(s) 642
AcuI CTGAAG 2 cut(s) 87, 255
AfiI CCNNNNNNNGG 1 cut(s) 390
AflIII ACRYGT 1 cut(s) 487
AgsI TTSAA 3 cut(s) 158, 164, 251
AjnI CCWGG 1 cut(s) 407
AluBI AGCT 6 cut(s) 58, 172, 218, 233, 254, 500
AluI AGCT 6 cut(s) 58, 172, 218, 233, 254, 500
Alw21I GWGCWC 1 cut(s) 720
Alw26I GTCTC 1 cut(s) 704
AlwI GGATC 1 cut(s) 95
Ama87I CYCGRG 1 cut(s) 622
AoxI GGCC 3 cut(s) 300, 396, 626
ApeKI GCWGC 2 cut(s) 262, 425
ApoI RAATTY 1 cut(s) 642
ArsI GACNNNNNNTTYG 4 cut(s) 368, 400, 452, 484
Asp700I GAANNNNTTC 1 cut(s) 72
AspS9I GGNCC 2 cut(s) 301, 460
AvaI CYCGRG 1 cut(s) 622
AvaII GGWCC 1 cut(s) 460
AxyI CCTNAGG 1 cut(s) 319
BalI TGGCCA 1 cut(s) 398
Bbv12I GWGCWC 1 cut(s) 720
BbvI GCAGC 2 cut(s) 249, 437
BccI CCATC 3 cut(s) 280, 671, 683
BciT130I CCWGG 1 cut(s) 409
BcoDI GTCTC 1 cut(s) 704
BfaI CTAG 1 cut(s) 53
BfmI CTRYAG 1 cut(s) 372
BisI GCNGC 2 cut(s) 263, 426
BlsI GCNGC 2 cut(s) 264, 427
Bme1390I CCNGG 1 cut(s) 409
Bme18I GGWCC 1 cut(s) 460
BmeT110I CYCGRG 1 cut(s) 622
BmgT120I GGNCC 2 cut(s) 301, 460
BmrFI CCNGG 1 cut(s) 409
BpuEI CTTGAG 1 cut(s) 704
BsaBI GATNNNNATC 1 cut(s) 8
BsaJI CCNNGG 4 cut(s) 34, 407, 408, 623
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bsc4I CCNNNNNNNGG 1 cut(s) 390
Bse21I CCTNAGG 1 cut(s) 319
Bse3DI GCAATG 1 cut(s) 104
Bse8I GATNNNNATC 1 cut(s) 8
BseBI CCWGG 1 cut(s) 409
BseDI CCNNGG 4 cut(s) 34, 407, 408, 623
BseGI GGATG 2 cut(s) 295, 454
BseJI GATNNNNATC 1 cut(s) 8
BseLI CCNNNNNNNGG 1 cut(s) 390
BseMI GCAATG 1 cut(s) 104
BseMII CTCAG 2 cut(s) 333, 510
BseXI GCAGC 2 cut(s) 249, 437
BsgI GTGCAG 2 cut(s) 248, 524
BshFI GGCC 3 cut(s) 302, 398, 628
BsiHKAI GWGCWC 1 cut(s) 720
BsiHKCI CYCGRG 1 cut(s) 622
BsiSI CCGG 1 cut(s) 210
BslFI GGGAC 1 cut(s) 605
BslI CCNNNNNNNGG 1 cut(s) 390
BsmAI GTCTC 1 cut(s) 704
BsmFI GGGAC 1 cut(s) 605
BsnI GGCC 3 cut(s) 302, 398, 628
BsoBI CYCGRG 1 cut(s) 622
Bsp1286I GDGCHC 1 cut(s) 720
Bsp143I GATC 1 cut(s) 100
Bsp19I CCATGG 1 cut(s) 34
BspACI CCGC 1 cut(s) 458
BspANI GGCC 3 cut(s) 302, 398, 628
BspCNI CTCAG 2 cut(s) 332, 509
BspHI TCATGA 1 cut(s) 706
BspPI GGATC 1 cut(s) 95
BspQI GCTCTTC 1 cut(s) 162
BsrDI GCAATG 1 cut(s) 104
BssECI CCNNGG 4 cut(s) 34, 407, 408, 623
BssMI GATC 1 cut(s) 100
BssT1I CCWWGG 1 cut(s) 34
Bst2UI CCWGG 1 cut(s) 409
Bst4CI ACNGT 2 cut(s) 553, 715
Bst6I CTCTTC 1 cut(s) 162
BstAPI GCANNNNNTGC 1 cut(s) 434
BstC8I GCNNGC 1 cut(s) 174
BstDEI CTNAG 2 cut(s) 319, 496
BstDSI CCRYGG 1 cut(s) 34
BstF5I GGATG 2 cut(s) 295, 454
BstKTI GATC 1 cut(s) 103
BstMAI GTCTC 1 cut(s) 704
BstMBI GATC 1 cut(s) 100
BstMWI GCNNNNNNNGC 2 cut(s) 215, 434
BstNI CCWGG 1 cut(s) 409
BstNSI RCATGY 2 cut(s) 491, 660
BstSCI CCNGG 1 cut(s) 407
BstSFI CTRYAG 1 cut(s) 372
BstV1I GCAGC 2 cut(s) 249, 437
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
Bsu36I CCTNAGG 1 cut(s) 319
BsuRI GGCC 3 cut(s) 302, 398, 628
BtgI CCRYGG 1 cut(s) 34
BtsCI GGATG 2 cut(s) 295, 454
BtsI GCAGTG 1 cut(s) 512
BtsIMutI CAGTG 1 cut(s) 512
Cac8I GCNNGC 1 cut(s) 174
CciI TCATGA 1 cut(s) 706
Cfr13I GGNCC 2 cut(s) 301, 460
CseI GACGC 1 cut(s) 584
CviAII CATG 8 cut(s) 35, 445, 488, 514, 579, 657, 707, 754
DdeI CTNAG 2 cut(s) 319, 496
DpnI GATC 1 cut(s) 102
DpnII GATC 1 cut(s) 100
EaeI YGGCCR 1 cut(s) 396
Eam1104I CTCTTC 1 cut(s) 162
EarI CTCTTC 1 cut(s) 162
Eco130I CCWWGG 1 cut(s) 34
Eco32I GATATC 1 cut(s) 88
Eco47I GGWCC 1 cut(s) 460
Eco57I CTGAAG 2 cut(s) 87, 255
Eco81I CCTNAGG 1 cut(s) 319
Eco88I CYCGRG 1 cut(s) 622
EcoRI GAATTC 1 cut(s) 642
EcoRII CCWGG 1 cut(s) 407
EcoRV GATATC 1 cut(s) 88
EcoT14I CCWWGG 1 cut(s) 34
ErhI CCWWGG 1 cut(s) 34
FaeI CATG 8 cut(s) 38, 448, 491, 517, 582, 660, 710, 757
FalI AAGNNNNNCTT 2 cut(s) 156, 188
FaqI GGGAC 1 cut(s) 605
FatI CATG 8 cut(s) 34, 444, 487, 513, 578, 656, 706, 753
FblI GTMKAC 1 cut(s) 562
Fnu4HI GCNGC 2 cut(s) 263, 426
FokI GGATG 2 cut(s) 302, 461
Fsp4HI GCNGC 2 cut(s) 263, 426
FspBI CTAG 1 cut(s) 53
GluI GCNGC 2 cut(s) 263, 426
HaeIII GGCC 3 cut(s) 302, 398, 628
HapII CCGG 1 cut(s) 210
HgaI GACGC 1 cut(s) 584
Hin1II CATG 8 cut(s) 38, 448, 491, 517, 582, 660, 710, 757
HinfI GANTC 4 cut(s) 198, 380, 441, 596
HpaII CCGG 1 cut(s) 210
Hpy166II GTNNAC 2 cut(s) 241, 563
Hpy188III TCNNGA 2 cut(s) 622, 707
Hpy8I GTNNAC 2 cut(s) 241, 563
Hpy99I CGWCG 1 cut(s) 671
HpyAV CCTTC 3 cut(s) 394, 577, 617
HpyCH4III ACNGT 2 cut(s) 553, 715
HpyCH4IV ACGT 1 cut(s) 669
HpyCH4V TGCA 5 cut(s) 176, 265, 356, 505, 656
HpyF10VI GCNNNNNNNGC 2 cut(s) 215, 434
HpyF3I CTNAG 2 cut(s) 319, 496
HpySE526I ACGT 1 cut(s) 669
Hsp92II CATG 8 cut(s) 38, 448, 491, 517, 582, 660, 710, 757
Kzo9I GATC 1 cut(s) 100
LguI GCTCTTC 1 cut(s) 162
LpnPI CCDG 6 cut(s) 64, 123, 223, 306, 394, 421
Lsp1109I GCAGC 2 cut(s) 249, 437
MaeI CTAG 1 cut(s) 53
MaeII ACGT 1 cut(s) 669
MaeIII GTNAC 2 cut(s) 529, 572
MalI GATC 1 cut(s) 102
MboI GATC 1 cut(s) 100
MboII GAAGA 6 cut(s) 59, 176, 179, 548, 551, 698
MfeI CAATTG 1 cut(s) 357
MflI RGATCY 1 cut(s) 100
MhlI GDGCHC 1 cut(s) 720
MlsI TGGCCA 1 cut(s) 398
MluCI AATT 5 cut(s) 159, 357, 642, 649, 732
MluNI TGGCCA 1 cut(s) 398
MlyI GAGTC 2 cut(s) 374, 435
MnlI CCTC 8 cut(s) 23, 175, 328, 339, 466, 476, 614, 618
Mox20I TGGCCA 1 cut(s) 398
MroXI GAANNNNTTC 1 cut(s) 72
MscI TGGCCA 1 cut(s) 398
MseI TTAA 1 cut(s) 648
Msp20I TGGCCA 1 cut(s) 398
MspA1I CMGCKG 1 cut(s) 500
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 1 cut(s) 409
MunI CAATTG 1 cut(s) 357
MvaI CCWGG 1 cut(s) 409
MwoI GCNNNNNNNGC 2 cut(s) 215, 434
NcoI CCATGG 1 cut(s) 34
NdeII GATC 1 cut(s) 100
NlaIII CATG 8 cut(s) 38, 448, 491, 517, 582, 660, 710, 757
NmuCI GTSAC 2 cut(s) 529, 572
NspI RCATGY 2 cut(s) 491, 660
PagI TCATGA 1 cut(s) 706
PasI CCCWGGG 1 cut(s) 408
PciI ACATGT 1 cut(s) 487
PciSI GCTCTTC 1 cut(s) 162
PcsI WCGNNNNNNNCGW 1 cut(s) 384
PdmI GAANNNNTTC 1 cut(s) 72
PfeI GAWTC 2 cut(s) 198, 596
PkrI GCNGC 2 cut(s) 264, 427
PleI GAGTC 2 cut(s) 374, 435
PpsI GAGTC 2 cut(s) 374, 435
PscI ACATGT 1 cut(s) 487
PsiI TTATAA 2 cut(s) 132, 731
Psp6I CCWGG 1 cut(s) 407
PspGI CCWGG 1 cut(s) 407
PspPI GGNCC 2 cut(s) 301, 460
PsuI RGATCY 1 cut(s) 100
PvuII CAGCTG 1 cut(s) 500
SapI GCTCTTC 1 cut(s) 162
SaqAI TTAA 1 cut(s) 648
SatI GCNGC 2 cut(s) 263, 426
Sau3AI GATC 1 cut(s) 100
Sau96I GGNCC 2 cut(s) 301, 460
SchI GAGTC 2 cut(s) 374, 435
ScrFI CCNGG 1 cut(s) 409
SduI GDGCHC 1 cut(s) 720
SetI ASST 9 cut(s) 60, 83, 174, 220, 235, 256, 320, 502, 672
SfcI CTRYAG 1 cut(s) 372
SinI GGWCC 1 cut(s) 460
SmlI CTYRAG 1 cut(s) 719
SmoI CTYRAG 1 cut(s) 719
Sse9I AATT 5 cut(s) 159, 357, 642, 649, 732
SsiI CCGC 1 cut(s) 458
SspMI CTAG 1 cut(s) 53
StyD4I CCNGG 1 cut(s) 407
StyI CCWWGG 1 cut(s) 34
TaaI ACNGT 2 cut(s) 553, 715
TaiI ACGT 1 cut(s) 672
TaqI TCGA 6 cut(s) 15, 45, 196, 387, 439, 666
TasI AATT 5 cut(s) 159, 357, 642, 649, 732
TfiI GAWTC 2 cut(s) 198, 596
Tru1I TTAA 1 cut(s) 648
Tru9I TTAA 1 cut(s) 648
TscAI CASTG 1 cut(s) 512
TseFI GTSAC 2 cut(s) 529, 572
TseI GCWGC 2 cut(s) 262, 425
Tsp45I GTSAC 2 cut(s) 529, 572
TspDTI ATGAA 7 cut(s) 134, 236, 306, 530, 534, 663, 699
TspRI CASTG 1 cut(s) 512
VpaK11BI GGWCC 1 cut(s) 460
XapI RAATTY 1 cut(s) 642
XceI RCATGY 2 cut(s) 491, 660
XmiI GTMKAC 1 cut(s) 562
XmnI GAANNNNTTC 1 cut(s) 72
XspI CTAG 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.