FvH4_4g27790

(-)-germacrene D synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000053
Physical Location & Seq
Reverse (-)
28631415 .. 28634981
3567 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27790.t1

Sequence Viewer

Length: 2154 bp
ATGGCTCTTCATCAAGTAGCTTCAACTGGTTCTAGAAACAAAATGGCTCTTCATCAAGTTTTAGCATCTCCAGCTCAAACCCCGCATGCTGCTTTCGATGTTAAACCACGTCGTGCTATTTATGCTCCTTGCTTATTGGGCGATCATTTTCTCTCATATTCTACTATGGATGTCGACGTCAAACTCAACCAACGTGTCCAAGGACTGAAAGAAGAAGTGAAGAGAATGTTAATGGCTGCAGTTAACCAGCCTGCTTCGCACATGTTAGACATGGTTGATAACATTCAGCGTTTAGGCTTGTCGTACCATTTTGAAAATGAGATTGATACAATCTTGAAACACATTCATGATCATTCTTATGGACGTAAAGATCTTGAAAATAATGGTGGCCTTTACACTACTGCTCTCCGTTTTCGATTGCTTCGACAGCAAGGCTATAATGTTTCAAGCGATGTGTTCAACAAGTTCAGGCAAAGTAACAGGACATTTAAGGAACATCTTATGAGCCATGTAGTAGGACTGTTGAGCTTGTATGAAGCCACTCACCTTAGGGTTCACGGAGAAGACATACTTGAAGAGGCACTAACCTTCACCACGAGCCATCTCGAATCTGCAGCACCCCGGTTGAGCTCCCCTTTGTCGAAACAAGTAACTCATGCCTTGTATCAACCACTTTGGAAGGGAGTTTCAAGGCTAGAAGCCAGGCATTACTTGTCTGTCTATGAGGAAGATGATTCACACAACCAAACTCTGTTAACTCTTGCAAAGTTGGATTTCAACTTAGCACATAAAGTCTATCAGAAAGAACCAAGTGATACGACAAGGTGGTGGAAGGACTTGGACTTTGTAAACAAGCTACCTTTTGCAAGAGACAGGTTAGTTGAGGCCTACTTCTGGAGTTTGGCGACATGCATTAATGAACCTGAATATTACTTTGCTAGAATTGTGGTGTGCAAGTATGTTGCTTTAACGACAGTCCTTGACGACGTTTACGATAACTTCAGCACATACGAAGAACTAGAGCGCTTTACAGAAGCTATTGAAAGGTGGGACATTTCTGCTGTTGATCAACTGCAATTGCCCCGGTGTATAGAGGTTTGCTATAAGGGATTGTTGGATACCTACTCAGGATTTGAAGAAAAGCTTGCAGATGAAGGAAACTTATATCTCATTGAGTATGCAAAAGAGGCAATCAAAACTCTACTTAGGGGTTACTTCCAAGAAGCCAAATGGTTGAAGGAAAAATACACACCAACAATGGATGAATTTATGCCCAATGGACTTGTTACGTCCTTCTTTACTATGGCAATCACAGCCTTTGTTGGAATGCGAGGTCTTGTTACAAAAGACTCCATGGATTGGATGTTAAATGACCCTAAGATTCTTAAGAGCAAGTCCACCGGTGGGAAAAAAATCCAACCCAGATTGGATCCACATGGGCAAAGTGAGTTCTGTCCACCTCAGCCCAGGCCACACACGTCGACGCCGGAGGAGGCCTGGTGCACTCGCCTCCCTTTCTTCGACGCGGGAGGAGGCCTGGTCCAAGGCCGGAGGAGGCTTGGTGCACTCGCTGCCGGGTTGGTGCAAGGCAGGGGGCTGCTTGCTGCTGTGCGTGCGGAGGAGGTTGGGCGTGCGGAGGACGCCAGAGGGCTGCCGGGATGGTTGCGCGCCTGGTCCAAGGCCGGAGGAGGCCTGGTGCACTCGCTGCCGGAATGGGTGCTCACCGGCGGCAGCCCAGAGAAGAAATATGGGTGCCCAAAGAAGAAATACAGCAACCCAGAGAAAAAGGAGAAGAAAGAGCAGAGAAAAAAGAGAAGAAAGAGAAGAACTACGGCAACCCAAAGAAAAGCTACATCGCTAATTGGCAGACTCCTCAACGACTTGGCTGGCCACAAGTTTGAGCAAAACAACGAGCAGGGTGCCTCAACAGTAGAATGCTACATGAAGCAATATGGCGTCGGAGAAGAAGAAGCGAAAATCGCGCTAACTAAGCAAAAGGATGCTGCATGGAAAGACCTAAACCAAGAGTTGCTCGATCTCCAATCCAACAATAGTATCCCCAAGCCCCTGCAGATTCATTTGAATCTTGCACGTTCTACTGAAGTTCTTTACAGTAATAATAACGAAAGGTATACCAACTCAAGGGTTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

718

Amino Acids

81.78

Weight (kDa)

8.79

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 47 - 221 2.5e-58 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 278 - 467 7.4e-61 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 319 - 461 3.5e-29 Terpene synthase family 2, C-terminal metal binding
Terpene_syn_C_2 PF19086 615 - 672 3e-12 Terpene synthase family 2, C-terminal metal binding
Terpene_synth_C PF03936 616 - 672 7.5e-15 Terpene synthase family, metal binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 180
AccB1I GGYRCC 2 cut(s) 1752, 1919
AccB7I CCANNNNNTGG 1 cut(s) 1359
AccI GTMKAC 3 cut(s) 174, 1481, 2132
AccII CGCG 3 cut(s) 1526, 1668, 1982
AciI CCGC 5 cut(s) 83, 1526, 1616, 1634, 1728
AclWI GGATC 2 cut(s) 1424, 1437
AcoI YGGCCR 1 cut(s) 1888
AcsI RAATTY 1 cut(s) 1265
AcuI CTGAAG 2 cut(s) 985, 2121
AcyI GRCGYC 4 cut(s) 177, 1484, 1641, 1956
AdeI CACNNNGTG 1 cut(s) 113
AfaI GTAC 1 cut(s) 305
AfeI AGCGCT 1 cut(s) 1025
AfiI CCNNNNNNNGG 4 cut(s) 894, 1359, 1404, 2142
AflII CTTAAG 1 cut(s) 1385
AflIII ACRYGT 3 cut(s) 193, 261, 1476
AgeI ACCGGT 1 cut(s) 1400
AjiI CACGTC 2 cut(s) 110, 1479
AjnI CCWGG 6 cut(s) 701, 1466, 1496, 1536, 1670, 1692
AluBI AGCT 8 cut(s) 20, 74, 528, 630, 856, 1037, 1144, 1850
AluI AGCT 8 cut(s) 20, 74, 528, 630, 856, 1037, 1144, 1850
Alw21I GWGCWC 5 cut(s) 632, 1505, 1567, 1701, 1722
Alw26I GTCTC 1 cut(s) 864
Alw44I GTGCAC 3 cut(s) 1501, 1563, 1697
AlwI GGATC 2 cut(s) 1424, 1437
Aor51HI AGCGCT 1 cut(s) 1025
AoxI GGCC 9 cut(s) 388, 885, 1469, 1494, 1534, 1546, 1680, 1690, 1888
ApaLI GTGCAC 3 cut(s) 1501, 1563, 1697
ApoI RAATTY 1 cut(s) 1265
ArsI GACNNNNNNTTYG 2 cut(s) 827, 859
AseI ATTAAT 1 cut(s) 915
AsiGI ACCGGT 1 cut(s) 1400
AspLEI GCGC 4 cut(s) 1026, 1668, 1670, 1984
AspS9I GGNCC 2 cut(s) 1540, 1674
AsuC2I CCSGG 4 cut(s) 622, 1084, 1576, 1656
AsuHPI GGTGA 3 cut(s) 536, 583, 1714
AvaII GGWCC 2 cut(s) 1540, 1674
AxyI CCTNAGG 1 cut(s) 548
BaeGI GKGCMC 4 cut(s) 1505, 1567, 1701, 1757
BaeI ACNNNNGTAYC 2 cut(s) 2038, 2071
BalI TGGCCA 1 cut(s) 1890
BamHI GGATCC 1 cut(s) 1429
BanI GGYRCC 2 cut(s) 1752, 1919
BanII GRGCYC 1 cut(s) 632
BarI GAAGNNNNNNTAC 2 cut(s) 1229, 1261
BauI CACGAG 1 cut(s) 595
BbsI GAAGAC 1 cut(s) 570
Bbv12I GWGCWC 5 cut(s) 632, 1505, 1567, 1701, 1722
BbvCI CCTCAGC 1 cut(s) 1461
BccI CCATC 2 cut(s) 609, 1653
BceAI ACGGC 1 cut(s) 1848
BcgI CGANNNNNNTGC 2 cut(s) 1901, 1935
BciT130I CCWGG 6 cut(s) 703, 1468, 1498, 1538, 1672, 1694
BciVI GTATCC 2 cut(s) 1111, 2066
BclI TGATCA 2 cut(s) 349, 1066
BcnI CCSGG 4 cut(s) 622, 1084, 1576, 1656
BcoDI GTCTC 1 cut(s) 864
BfaI CTAG 4 cut(s) 33, 695, 939, 1019
BfmI CTRYAG 3 cut(s) 237, 612, 2069
BfoI RGCGCY 1 cut(s) 1027
BfrI CTTAAG 1 cut(s) 1385
BfuI GTATCC 2 cut(s) 1111, 2066
BglII AGATCT 1 cut(s) 370
Bme18I GGWCC 2 cut(s) 1540, 1674
BmgBI CACGTC 2 cut(s) 110, 1479
BmgT120I GGNCC 2 cut(s) 1540, 1674
BmiI GGNNCC 3 cut(s) 1431, 1754, 1921
BmsI GCATC 2 cut(s) 74, 1990
BpiI GAAGAC 1 cut(s) 570
BpmI CTGGAG 2 cut(s) 54, 916
Bpu10I CCTNAGC 1 cut(s) 1461
BpuEI CTTGAG 1 cut(s) 2125
BpuMI CCSGG 4 cut(s) 622, 1084, 1576, 1656
BsaHI GRCGYC 4 cut(s) 177, 1484, 1641, 1956
BsaJI CCNNGG 7 cut(s) 199, 620, 1082, 1353, 1466, 1543, 1677
BsaWI WCCGGW 1 cut(s) 1400
BsaXI ACNNNNNCTCC 8 cut(s) 1484, 1514, 1524, 1546, 1554, 1576, 1680, 1710
Bsc4I CCNNNNNNNGG 4 cut(s) 894, 1359, 1404, 2142
Bse118I RCCGGY 2 cut(s) 1400, 1724
Bse1I ACTGG 1 cut(s) 31
Bse21I CCTNAGG 1 cut(s) 548
BseBI CCWGG 6 cut(s) 703, 1468, 1498, 1538, 1672, 1694
BseDI CCNNGG 7 cut(s) 199, 620, 1082, 1353, 1466, 1543, 1677
BseGI GGATG 5 cut(s) 175, 1267, 1368, 1664, 2005
BseLI CCNNNNNNNGG 4 cut(s) 894, 1359, 1404, 2142
BseMII CTCAG 2 cut(s) 1140, 1475
BseNI ACTGG 1 cut(s) 31
BsePI GCGCGC 1 cut(s) 1666
BseRI GAGGAG 6 cut(s) 1505, 1545, 1567, 1634, 1701, 1862
BseSI GKGCMC 4 cut(s) 1505, 1567, 1701, 1757
Bsh1236I CGCG 3 cut(s) 1526, 1668, 1982
BshFI GGCC 9 cut(s) 390, 887, 1471, 1496, 1536, 1548, 1682, 1692, 1890
BshNI GGYRCC 2 cut(s) 1752, 1919
BshTI ACCGGT 1 cut(s) 1400
BsiHKAI GWGCWC 5 cut(s) 632, 1505, 1567, 1701, 1722
BslFI GGGAC 1 cut(s) 1064
BslI CCNNNNNNNGG 4 cut(s) 894, 1359, 1404, 2142
BsmAI GTCTC 1 cut(s) 864
BsmFI GGGAC 1 cut(s) 1064
BsmI GAATGC 2 cut(s) 1332, 1940
BsnI GGCC 9 cut(s) 390, 887, 1471, 1496, 1536, 1548, 1682, 1692, 1890
Bsp1286I GDGCHC 6 cut(s) 632, 1505, 1567, 1701, 1722, 1757
Bsp143I GATC 6 cut(s) 142, 349, 370, 1066, 1429, 2035
Bsp19I CCATGG 1 cut(s) 1353
BspACI CCGC 5 cut(s) 83, 1526, 1616, 1634, 1728
BspANI GGCC 9 cut(s) 390, 887, 1471, 1496, 1536, 1548, 1682, 1692, 1890
BspCNI CTCAG 2 cut(s) 1139, 1474
BspFNI CGCG 3 cut(s) 1526, 1668, 1982
BspHI TCATGA 1 cut(s) 346
BspLI GGNNCC 3 cut(s) 1431, 1754, 1921
BspMAI CTGCAG 3 cut(s) 241, 616, 2073
BspPI GGATC 2 cut(s) 1424, 1437
BspQI GCTCTTC 2 cut(s) 12, 54
BspT107I GGYRCC 2 cut(s) 1752, 1919
BspTI CTTAAG 1 cut(s) 1385
BsrFI RCCGGY 2 cut(s) 1400, 1724
BsrI ACTGG 1 cut(s) 31
BssAI RCCGGY 2 cut(s) 1400, 1724
BssECI CCNNGG 7 cut(s) 199, 620, 1082, 1353, 1466, 1543, 1677
BssHII GCGCGC 1 cut(s) 1666
BssMI GATC 6 cut(s) 142, 349, 370, 1066, 1429, 2035
BssNAI GTATAC 1 cut(s) 2133
BssNI GRCGYC 4 cut(s) 177, 1484, 1641, 1956
BssSI CACGAG 1 cut(s) 595
BssT1I CCWWGG 4 cut(s) 199, 1353, 1543, 1677
Bst1107I GTATAC 1 cut(s) 2133
Bst2BI CACGAG 1 cut(s) 595
Bst2UI CCWGG 6 cut(s) 703, 1468, 1498, 1538, 1672, 1694
Bst4CI ACNGT 4 cut(s) 522, 976, 1930, 2114
Bst6I CTCTTC 4 cut(s) 12, 54, 215, 570
BstACI GRCGYC 4 cut(s) 177, 1484, 1641, 1956
BstAFI CTTAAG 1 cut(s) 1385
BstAPI GCANNNNNTGC 2 cut(s) 1571, 1705
BstC8I GCNNGC 8 cut(s) 87, 252, 1146, 1602, 1614, 1632, 1668, 1888
BstDEI CTNAG 7 cut(s) 548, 781, 1126, 1205, 1377, 1461, 1989
BstDSI CCRYGG 1 cut(s) 1353
BstF5I GGATG 5 cut(s) 175, 1267, 1368, 1664, 2005
BstFNI CGCG 3 cut(s) 1526, 1668, 1982
BstH2I RGCGCY 1 cut(s) 1027
BstHHI GCGC 4 cut(s) 1026, 1668, 1670, 1984
BstKTI GATC 6 cut(s) 145, 352, 373, 1069, 1432, 2038
BstMAI GTCTC 1 cut(s) 864
BstMBI GATC 6 cut(s) 142, 349, 370, 1066, 1429, 2035
BstNI CCWGG 6 cut(s) 703, 1468, 1498, 1538, 1672, 1694
BstNSI RCATGY 3 cut(s) 89, 265, 912
BstSFI CTRYAG 3 cut(s) 237, 612, 2069
BstSLI GKGCMC 4 cut(s) 1505, 1567, 1701, 1757
BstUI CGCG 3 cut(s) 1526, 1668, 1982
BstV2I GAAGAC 1 cut(s) 570
BstX2I RGATCY 2 cut(s) 370, 1429
BstYI RGATCY 2 cut(s) 370, 1429
BstZ17I GTATAC 1 cut(s) 2133
Bsu36I CCTNAGG 1 cut(s) 548
BsuI GTATCC 2 cut(s) 1111, 2066
BsuRI GGCC 9 cut(s) 390, 887, 1471, 1496, 1536, 1548, 1682, 1692, 1890
BtgI CCRYGG 1 cut(s) 1353
BtgZI GCGATG 2 cut(s) 465, 1839
BtrI CACGTC 2 cut(s) 110, 1479
BtsCI GGATG 5 cut(s) 175, 1267, 1368, 1664, 2005
Cac8I GCNNGC 8 cut(s) 87, 252, 1146, 1602, 1614, 1632, 1668, 1888
CciI TCATGA 1 cut(s) 346
CfoI GCGC 4 cut(s) 1026, 1668, 1670, 1984
Cfr10I RCCGGY 2 cut(s) 1400, 1724
Cfr13I GGNCC 2 cut(s) 1540, 1674
CseI GACGC 4 cut(s) 1492, 1532, 1649, 1945
Csp6I GTAC 1 cut(s) 304
CspAI ACCGGT 1 cut(s) 1400
CviQI GTAC 1 cut(s) 304
DdeI CTNAG 7 cut(s) 548, 781, 1126, 1205, 1377, 1461, 1989
DpnI GATC 6 cut(s) 144, 351, 372, 1068, 1431, 2037
DpnII GATC 6 cut(s) 142, 349, 370, 1066, 1429, 2035
DraIII CACNNNGTG 1 cut(s) 113
EaeI YGGCCR 1 cut(s) 1888
Eam1104I CTCTTC 4 cut(s) 12, 54, 215, 570
EarI CTCTTC 4 cut(s) 12, 54, 215, 570
Ecl136II GAGCTC 1 cut(s) 630
Eco130I CCWWGG 4 cut(s) 199, 1353, 1543, 1677
Eco147I AGGCCT 4 cut(s) 887, 1496, 1536, 1692
Eco24I GRGCYC 1 cut(s) 632
Eco47I GGWCC 2 cut(s) 1540, 1674
Eco47III AGCGCT 1 cut(s) 1025
Eco53kI GAGCTC 1 cut(s) 630
Eco57I CTGAAG 2 cut(s) 985, 2121
Eco81I CCTNAGG 1 cut(s) 548
EcoICRI GAGCTC 1 cut(s) 630
EcoRII CCWGG 6 cut(s) 701, 1466, 1496, 1536, 1670, 1692
EcoT14I CCWWGG 4 cut(s) 199, 1353, 1543, 1677
EcoT22I ATGCAT 1 cut(s) 914
EcoT38I GRGCYC 1 cut(s) 632
ErhI CCWWGG 4 cut(s) 199, 1353, 1543, 1677
FalI AAGNNNNNCTT 4 cut(s) 555, 587, 1128, 1160
FaqI GGGAC 1 cut(s) 1064
FauI CCCGC 2 cut(s) 90, 1519
FbaI TGATCA 2 cut(s) 349, 1066
FblI GTMKAC 3 cut(s) 174, 1481, 2132
FokI GGATG 5 cut(s) 182, 1274, 1375, 1671, 2012
FriOI GRGCYC 1 cut(s) 632
FspBI CTAG 4 cut(s) 33, 695, 939, 1019
GlaI GCGC 4 cut(s) 1025, 1667, 1669, 1983
GsuI CTGGAG 2 cut(s) 54, 916
HaeII RGCGCY 1 cut(s) 1027
HaeIII GGCC 9 cut(s) 390, 887, 1471, 1496, 1536, 1548, 1682, 1692, 1890
HgaI GACGC 4 cut(s) 1492, 1532, 1649, 1945
HhaI GCGC 4 cut(s) 1026, 1668, 1670, 1984
Hin1I GRCGYC 4 cut(s) 177, 1484, 1641, 1956
Hin6I GCGC 4 cut(s) 1024, 1666, 1668, 1982
HinP1I GCGC 4 cut(s) 1024, 1666, 1668, 1982
HincII GTYRAC 4 cut(s) 175, 244, 756, 1482
HindII GTYRAC 4 cut(s) 175, 244, 756, 1482
HindIII AAGCTT 1 cut(s) 1142
HinfI GANTC 7 cut(s) 608, 734, 1349, 1381, 1869, 2074, 2083
HpaI GTTAAC 2 cut(s) 244, 756
HphI GGTGA 3 cut(s) 536, 583, 1714
Hpy188I TCNGA 2 cut(s) 801, 1961
Hpy188III TCNNGA 7 cut(s) 33, 334, 347, 374, 605, 895, 1128
Hpy99I CGWCG 7 cut(s) 114, 179, 989, 1483, 1486, 1526, 1961
HpyAV CCTTC 6 cut(s) 598, 673, 826, 1148, 1231, 1303
HpyCH4III ACNGT 4 cut(s) 522, 976, 1930, 2114
HpyCH4IV ACGT 8 cut(s) 109, 177, 193, 364, 987, 1289, 1478, 2092
HpyF3I CTNAG 7 cut(s) 548, 781, 1126, 1205, 1377, 1461, 1989
HpySE526I ACGT 8 cut(s) 109, 177, 193, 364, 987, 1289, 1478, 2092
Hsp92I GRCGYC 4 cut(s) 177, 1484, 1641, 1956
HspAI GCGC 4 cut(s) 1024, 1666, 1668, 1982
Ksp22I TGATCA 2 cut(s) 349, 1066
KspAI GTTAAC 2 cut(s) 244, 756
Kzo9I GATC 6 cut(s) 142, 349, 370, 1066, 1429, 2035
LguI GCTCTTC 2 cut(s) 12, 54
LmnI GCTCC 2 cut(s) 130, 635
LweI GCATC 2 cut(s) 74, 1990
MaeI CTAG 4 cut(s) 33, 695, 939, 1019
MaeII ACGT 8 cut(s) 109, 177, 193, 364, 987, 1289, 1478, 2092
MaeIII GTNAC 5 cut(s) 476, 649, 1211, 1285, 1339
MalI GATC 6 cut(s) 144, 351, 372, 1068, 1431, 2037
MboI GATC 6 cut(s) 142, 349, 370, 1066, 1429, 2035
MfeI CAATTG 1 cut(s) 1076
MflI RGATCY 2 cut(s) 370, 1429
MhlI GDGCHC 6 cut(s) 632, 1505, 1567, 1701, 1722, 1757
MlsI TGGCCA 1 cut(s) 1890
MluCI AATT 4 cut(s) 942, 1076, 1265, 1860
MluNI TGGCCA 1 cut(s) 1890
MlyI GAGTC 2 cut(s) 1343, 1863
MmeI TCCRAC 6 cut(s) 750, 1095, 1303, 1441, 1939, 2070
Mox20I TGGCCA 1 cut(s) 1890
Mph1103I ATGCAT 1 cut(s) 914
MscI TGGCCA 1 cut(s) 1890
MseI TTAA 9 cut(s) 102, 230, 243, 489, 755, 915, 968, 1367, 1386
MslI CAYNNNNRTG 2 cut(s) 345, 357
Msp20I TGGCCA 1 cut(s) 1890
MspCI CTTAAG 1 cut(s) 1385
MunI CAATTG 1 cut(s) 1076
Mva1269I GAATGC 2 cut(s) 1332, 1940
MvaI CCWGG 6 cut(s) 703, 1468, 1498, 1538, 1672, 1694
MvnI CGCG 3 cut(s) 1526, 1668, 1982
NciI CCSGG 4 cut(s) 622, 1084, 1576, 1656
NcoI CCATGG 1 cut(s) 1353
NdeII GATC 6 cut(s) 142, 349, 370, 1066, 1429, 2035
NlaIV GGNNCC 3 cut(s) 1431, 1754, 1921
NsiI ATGCAT 1 cut(s) 914
NspI RCATGY 3 cut(s) 89, 265, 912
PaeI GCATGC 1 cut(s) 89
PagI TCATGA 1 cut(s) 346
PauI GCGCGC 1 cut(s) 1666
PceI AGGCCT 4 cut(s) 887, 1496, 1536, 1692
PciI ACATGT 1 cut(s) 261
PciSI GCTCTTC 2 cut(s) 12, 54
PcsI WCGNNNNNNNCGW 2 cut(s) 421, 990
PctI GAATGC 2 cut(s) 1332, 1940
PfeI GAWTC 5 cut(s) 608, 734, 1381, 2074, 2083
PflMI CCANNNNNTGG 1 cut(s) 1359
PinAI ACCGGT 1 cut(s) 1400
PleI GAGTC 2 cut(s) 1343, 1863
PpsI GAGTC 2 cut(s) 1343, 1863
PscI ACATGT 1 cut(s) 261
PshBI ATTAAT 1 cut(s) 915
Psp124BI GAGCTC 1 cut(s) 632
Psp6I CCWGG 6 cut(s) 701, 1466, 1496, 1536, 1670, 1692
PspGI CCWGG 6 cut(s) 701, 1466, 1496, 1536, 1670, 1692
PspN4I GGNNCC 3 cut(s) 1431, 1754, 1921
PspPI GGNCC 2 cut(s) 1540, 1674
PstI CTGCAG 3 cut(s) 241, 616, 2073
PsuI RGATCY 2 cut(s) 370, 1429
PteI GCGCGC 1 cut(s) 1666
RsaI GTAC 1 cut(s) 305
RsaNI GTAC 1 cut(s) 304
RseI CAYNNNNRTG 2 cut(s) 345, 357
SacI GAGCTC 1 cut(s) 632
SalI GTCGAC 2 cut(s) 173, 1480
SapI GCTCTTC 2 cut(s) 12, 54
SaqAI TTAA 9 cut(s) 102, 230, 243, 489, 755, 915, 968, 1367, 1386
Sau3AI GATC 6 cut(s) 142, 349, 370, 1066, 1429, 2035
Sau96I GGNCC 2 cut(s) 1540, 1674
SchI GAGTC 2 cut(s) 1343, 1863
SduI GDGCHC 6 cut(s) 632, 1505, 1567, 1701, 1722, 1757
SfaNI GCATC 2 cut(s) 74, 1990
SfcI CTRYAG 3 cut(s) 237, 612, 2069
SgrAI CRCCGGYG 2 cut(s) 1400, 1724
SgrDI CGTCGACG 1 cut(s) 1480
SinI GGWCC 2 cut(s) 1540, 1674
SmiMI CAYNNNNRTG 2 cut(s) 345, 357
SmlI CTYRAG 2 cut(s) 1385, 2140
SmoI CTYRAG 2 cut(s) 1385, 2140
SphI GCATGC 1 cut(s) 89
Sse9I AATT 4 cut(s) 942, 1076, 1265, 1860
SseBI AGGCCT 4 cut(s) 887, 1496, 1536, 1692
SsiI CCGC 5 cut(s) 83, 1526, 1616, 1634, 1728
SspI AATATT 1 cut(s) 929
SspMI CTAG 4 cut(s) 33, 695, 939, 1019
SstI GAGCTC 1 cut(s) 632
StuI AGGCCT 4 cut(s) 887, 1496, 1536, 1692
StyI CCWWGG 4 cut(s) 199, 1353, 1543, 1677
TaaI ACNGT 4 cut(s) 522, 976, 1930, 2114
TaiI ACGT 8 cut(s) 112, 180, 196, 367, 990, 1292, 1481, 2095
TaqI TCGA 9 cut(s) 96, 174, 415, 424, 606, 641, 1481, 1521, 2034
TasI AATT 4 cut(s) 942, 1076, 1265, 1860
TauI GCSGC 1 cut(s) 1731
TfiI GAWTC 5 cut(s) 608, 734, 1381, 2074, 2083
Tru1I TTAA 9 cut(s) 102, 230, 243, 489, 755, 915, 968, 1367, 1386
Tru9I TTAA 9 cut(s) 102, 230, 243, 489, 755, 915, 968, 1367, 1386
TspDTI ATGAA 9 cut(s) 41, 335, 549, 933, 1167, 1278, 1958, 2066, 2139
TspGWI ACGGA 2 cut(s) 398, 573
Van91I CCANNNNNTGG 1 cut(s) 1359
Vha464I CTTAAG 1 cut(s) 1385
VneI GTGCAC 3 cut(s) 1501, 1563, 1697
VpaK11BI GGWCC 2 cut(s) 1540, 1674
VspI ATTAAT 1 cut(s) 915
XapI RAATTY 1 cut(s) 1265
XbaI TCTAGA 1 cut(s) 32
XceI RCATGY 3 cut(s) 89, 265, 912
XmiI GTMKAC 3 cut(s) 174, 1481, 2132
XspI CTAG 4 cut(s) 33, 695, 939, 1019
ZraI GACGTC 1 cut(s) 178
Zsp2I ATGCAT 1 cut(s) 914
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.