Rw5G027440

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000053
Physical Location & Seq
Reverse (-)
40550966 .. 40553413
2448 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G027440.1

Sequence Viewer

Length: 1629 bp
ATGTCTATGGCTACAGATCCTTCCCCATCAAGTCAAGTACCACAAATTGCTAAGCCTGAAATTGTTCGCCAGACGGCGAATTTTCAACCAAGCATTTGGGGAGATCAGTTCATCAACTATGATTCTCAAGACATTAAGACTGAGGCCCTTTGGCAACAAGAAGTCGAGGAACTGAAAGTAGTAGTGAAGAGAGAAGTATTTACAAATGGGAAAGGTGATTTTTCCCATCAACTCAAGTTGATCGATGCAATCCAACGCCTTGGCGTGGCATACCATTTTGAACAAGAAATAGAATATGCACTGCAACACATCCATGCTACATATCATGATCAGGATCACTACCGTGATAATGGCGATCTTTACACTACTGCGCTATGTTTTCGATTGCTAAGGCAACAGGGATATGATATCTCATCTGATATATTCAACAAGTTCAAAGATGCAAATGGTAACTTCAAAGAAGGCTTAGTTGTCGATGGGTCAGCTATGCTGAGCCTCTACGAAGCTGCACATCTTAAGGTTCACGGAGAAGATATACTAAAGAGGCTCTTGTTTTCACCACTACTCACCTCGATACAAATGACTGATCAAATCACTCAAGCCTTGGCGAGACCTCTGCGAAAAAGTCTAGAGAGGATATGTGCCAAGCGTCACATGTCAATTTACCAACATGAAGATGAAGCCTCACTCATCAACCACAATGAAGCTTTATTGAGACTTGCAAAGTTGGATTTCAATCTTGTTCAGTCTTTACACAAAAAAGAGTTGTGTGAAATTAGTAGGTGGTGGAAAGAACTAGACTTTGAAAGGAAATTGCCTTTTGCAAGAGACAGGAAGGTGGAGTTGTACTTTTGGATAGTGGGAGTATATTTTGAACCCCAGTACTCTATTGGCAGAACCATTATGACAAAAGTGTCTATCCTCCTAACTGTTTTGGATGATATATATGACGCATATGGTACATTTGAAGAACTCGTGATCTTTACTGAAGCAATTGACAGGTGGGATGTCAAATGCATAGATGAACTCCCGGACTATTTGAAAATATTCTATCATGAACTTTTGAATCTTTTCAATGATATTGACAAAGTGATGTCAAAGGAAGGAAAATCTTACCGAGTTTGCTACGCAATACAAGCTATGAAAAATCAAGCTCGATCATATTTCAACGAGGCCCAATGGTTGCACGAAGGACGCACTCCAAGCATTGAGGAGTATATGAGGGTTGCAACAGTTTCTATTAGTTACACTTTTCTAACAACCATTTCATTACTCGGCATGGGAGATATTGTAACGAAGGATGCATTTGAGTGGTTGTTGAATGACCCGAAAATTTTTGAGAAAGAGAGAGAGCATGCACCTTCTAGCATTGATTGCTATATGAAGCAATATAGTGTTTCAGAGCAAGAGACAATTGATATTTTCCACAAACAAATTGTGGAATCATGGAAGGATATAAACGAGGAGTTTCGTAAACCAACCGCCGTGCCAATATCGGTTCTTATGCGCGTTCTCAATTTAACAAGAGTTGCGGATCTCCTTTATAAGGGAGAAGATGGATTCACACGGGTTGGGAAGATGACCAAAGATAGTGTTGCTGCAGTTATTATCGATCCAGTGCCACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

542

Amino Acids

63.06

Weight (kDa)

5.43

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 32 - 183 1.6e-49 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 264 - 447 2.7e-72 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 304 - 485 3e-41 Terpene synthase family 2, C-terminal metal binding
Terpene_synth_C PF03936 446 - 485 1e-07 Terpene synthase family, metal binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1545
AasI GACNNNNNNGTC 1 cut(s) 913
AccII CGCG 1 cut(s) 1509
AciI CCGC 2 cut(s) 1482, 1532
AclWI GGATC 4 cut(s) 11, 342, 1542, 1607
AcsI RAATTY 2 cut(s) 79, 1332
AcuI CTGAAG 1 cut(s) 1008
AfaI GTAC 4 cut(s) 39, 848, 884, 961
AfiI CCNNNNNNNGG 2 cut(s) 265, 1546
AflII CTTAAG 1 cut(s) 515
AflIII ACRYGT 1 cut(s) 654
AleI CACNNNNGTG 1 cut(s) 342
AluBI AGCT 5 cut(s) 485, 506, 707, 1139, 1154
AluI AGCT 5 cut(s) 485, 506, 707, 1139, 1154
Alw26I GTCTC 4 cut(s) 604, 709, 822, 1403
AlwI GGATC 4 cut(s) 11, 342, 1542, 1607
AoxI GGCC 2 cut(s) 144, 1173
ApeKI GCWGC 2 cut(s) 506, 1598
ApoI RAATTY 2 cut(s) 79, 1332
Asp700I GAANNNNTTC 3 cut(s) 63, 1046, 1070
AspLEI GCGC 2 cut(s) 373, 1509
AspS9I GGNCC 2 cut(s) 145, 1174
AsuC2I CCSGG 1 cut(s) 1031
AsuHPI GGTGA 3 cut(s) 227, 549, 559
BauI CACGAG 1 cut(s) 974
BbvI GCAGC 2 cut(s) 493, 1585
BccI CCATC 4 cut(s) 34, 234, 470, 1550
BceAI ACGGC 2 cut(s) 90, 1469
BcgI CGANNNNNNTGC 2 cut(s) 598, 632
BclI TGATCA 2 cut(s) 328, 586
BcnI CCSGG 1 cut(s) 1031
BcoDI GTCTC 4 cut(s) 604, 709, 822, 1403
BfaI CTAG 3 cut(s) 629, 797, 1365
BfmI CTRYAG 2 cut(s) 12, 1599
BfrI CTTAAG 1 cut(s) 515
BisI GCNGC 2 cut(s) 507, 1599
BlpI GCTNAGC 2 cut(s) 51, 491
BlsI GCNGC 2 cut(s) 508, 1600
BmcAI AGTACT 1 cut(s) 884
Bme1390I CCNGG 1 cut(s) 1031
BmgT120I GGNCC 2 cut(s) 145, 1174
BmrFI CCNGG 1 cut(s) 1031
BmrI ACTGGG 1 cut(s) 874
BmsI GCATC 3 cut(s) 235, 430, 1291
BmuI ACTGGG 1 cut(s) 874
Bpu10I CCTNAGC 1 cut(s) 389
Bpu1102I GCTNAGC 2 cut(s) 51, 491
BpuEI CTTGAG 3 cut(s) 111, 218, 582
BpuMI CCSGG 1 cut(s) 1031
Bsa29I ATCGAT 2 cut(s) 243, 1611
BsaBI GATNNNNATC 2 cut(s) 333, 735
BsaI GGTCTC 1 cut(s) 604
BsaJI CCNNGG 2 cut(s) 259, 603
BsaXI ACNNNNNCTCC 2 cut(s) 1275, 1305
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 1546
Bse1I ACTGG 2 cut(s) 880, 1616
Bse8I GATNNNNATC 2 cut(s) 333, 735
BseCI ATCGAT 2 cut(s) 243, 1611
BseDI CCNNGG 2 cut(s) 259, 603
BseGI GGATG 4 cut(s) 309, 943, 1012, 1306
BseJI GATNNNNATC 2 cut(s) 333, 735
BseLI CCNNNNNNNGG 2 cut(s) 265, 1546
BseMII CTCAG 2 cut(s) 132, 482
BseNI ACTGG 2 cut(s) 880, 1616
BseRI GAGGAG 2 cut(s) 1226, 1478
BseXI GCAGC 2 cut(s) 493, 1585
BsgI GTGCAG 1 cut(s) 492
Bsh1236I CGCG 1 cut(s) 1509
BshFI GGCC 2 cut(s) 146, 1175
BshVI ATCGAT 2 cut(s) 243, 1611
BsiSI CCGG 1 cut(s) 1031
BslI CCNNNNNNNGG 2 cut(s) 265, 1546
BsmAI GTCTC 4 cut(s) 604, 709, 822, 1403
BsnI GGCC 2 cut(s) 146, 1175
Bso31I GGTCTC 1 cut(s) 604
Bsp1720I GCTNAGC 2 cut(s) 51, 491
BspACI CCGC 2 cut(s) 1482, 1532
BspANI GGCC 2 cut(s) 146, 1175
BspCNI CTCAG 2 cut(s) 133, 483
BspDI ATCGAT 2 cut(s) 243, 1611
BspFNI CGCG 1 cut(s) 1509
BspHI TCATGA 2 cut(s) 325, 1054
BspMAI CTGCAG 1 cut(s) 1603
BspPI GGATC 4 cut(s) 11, 342, 1542, 1607
BspTI CTTAAG 1 cut(s) 515
BspTNI GGTCTC 1 cut(s) 604
BsrI ACTGG 2 cut(s) 880, 1616
BssECI CCNNGG 2 cut(s) 259, 603
BssSI CACGAG 1 cut(s) 974
BssT1I CCWWGG 2 cut(s) 259, 603
Bst2BI CACGAG 1 cut(s) 974
Bst4CI ACNGT 4 cut(s) 344, 931, 1234, 1626
Bst6I CTCTTC 1 cut(s) 182
BstAFI CTTAAG 1 cut(s) 515
BstAPI GCANNNNNTGC 1 cut(s) 1374
BstC8I GCNNGC 1 cut(s) 1356
BstDEI CTNAG 5 cut(s) 51, 141, 389, 466, 491
BstENI CCTNNNNNAGG 1 cut(s) 1544
BstF5I GGATG 4 cut(s) 309, 943, 1012, 1306
BstFNI CGCG 1 cut(s) 1509
BstHHI GCGC 2 cut(s) 373, 1509
BstMAI GTCTC 4 cut(s) 604, 709, 822, 1403
BstMWI GCNNNNNNNGC 3 cut(s) 1136, 1203, 1374
BstNSI RCATGY 2 cut(s) 658, 1358
BstSCI CCNGG 1 cut(s) 1029
BstSFI CTRYAG 2 cut(s) 12, 1599
BstUI CGCG 1 cut(s) 1509
BstV1I GCAGC 2 cut(s) 493, 1585
BstX2I RGATCY 2 cut(s) 16, 1534
BstXI CCANNNNNNTGG 2 cut(s) 96, 260
BstYI RGATCY 2 cut(s) 16, 1534
Bsu15I ATCGAT 2 cut(s) 243, 1611
BsuRI GGCC 2 cut(s) 146, 1175
BsuTUI ATCGAT 2 cut(s) 243, 1611
BtsCI GGATG 4 cut(s) 309, 943, 1012, 1306
BtsI GCAGTG 1 cut(s) 299
BtsIMutI CAGTG 3 cut(s) 299, 1622, 1623
Cac8I GCNNGC 1 cut(s) 1356
CciI TCATGA 2 cut(s) 325, 1054
CfoI GCGC 2 cut(s) 373, 1509
Cfr13I GGNCC 2 cut(s) 145, 1174
ClaI ATCGAT 2 cut(s) 243, 1611
CseI GACGC 3 cut(s) 638, 959, 1203
Csp6I GTAC 4 cut(s) 38, 847, 883, 960
CviAII CATG 8 cut(s) 314, 326, 655, 671, 1055, 1279, 1355, 1446
CviQI GTAC 4 cut(s) 38, 847, 883, 960
DdeI CTNAG 5 cut(s) 51, 141, 389, 466, 491
DrdI GACNNNNNNGTC 1 cut(s) 913
DseDI GACNNNNNNGTC 1 cut(s) 913
Eam1104I CTCTTC 1 cut(s) 182
EarI CTCTTC 1 cut(s) 182
Eco130I CCWWGG 2 cut(s) 259, 603
Eco31I GGTCTC 1 cut(s) 604
Eco32I GATATC 1 cut(s) 409
Eco57I CTGAAG 1 cut(s) 1008
EcoNI CCTNNNNNAGG 1 cut(s) 1544
EcoO109I RGGNCCY 1 cut(s) 145
EcoRV GATATC 1 cut(s) 409
EcoT14I CCWWGG 2 cut(s) 259, 603
EcoT22I ATGCAT 2 cut(s) 1019, 1306
ErhI CCWWGG 2 cut(s) 259, 603
FaeI CATG 8 cut(s) 317, 329, 658, 674, 1058, 1282, 1358, 1449
FalI AAGNNNNNCTT 2 cut(s) 533, 565
FatI CATG 8 cut(s) 313, 325, 654, 670, 1054, 1278, 1354, 1445
FauNDI CATATG 1 cut(s) 955
FbaI TGATCA 2 cut(s) 328, 586
Fnu4HI GCNGC 2 cut(s) 507, 1599
FokI GGATG 4 cut(s) 296, 950, 1019, 1313
Fsp4HI GCNGC 2 cut(s) 507, 1599
FspBI CTAG 3 cut(s) 629, 797, 1365
GlaI GCGC 2 cut(s) 372, 1508
GluI GCNGC 2 cut(s) 507, 1599
HaeIII GGCC 2 cut(s) 146, 1175
HapII CCGG 1 cut(s) 1031
HgaI GACGC 3 cut(s) 638, 959, 1203
HhaI GCGC 2 cut(s) 373, 1509
Hin1II CATG 8 cut(s) 317, 329, 658, 674, 1058, 1282, 1358, 1449
Hin6I GCGC 2 cut(s) 371, 1507
HinP1I GCGC 2 cut(s) 371, 1507
HindIII AAGCTT 1 cut(s) 705
HinfI GANTC 4 cut(s) 122, 1066, 1442, 1560
HpaII CCGG 1 cut(s) 1031
HphI GGTGA 3 cut(s) 227, 549, 559
Hpy166II GTNNAC 2 cut(s) 523, 1475
Hpy188I TCNGA 2 cut(s) 418, 1402
Hpy188III TCNNGA 6 cut(s) 128, 326, 332, 629, 976, 1055
Hpy8I GTNNAC 2 cut(s) 523, 1475
HpyAV CCTTC 8 cut(s) 30, 455, 829, 1097, 1184, 1291, 1371, 1444
HpyCH4III ACNGT 4 cut(s) 344, 931, 1234, 1626
HpyF10VI GCNNNNNNNGC 3 cut(s) 1136, 1203, 1374
HpyF3I CTNAG 5 cut(s) 51, 141, 389, 466, 491
Hsp92II CATG 8 cut(s) 317, 329, 658, 674, 1058, 1282, 1358, 1449
HspAI GCGC 2 cut(s) 371, 1507
Ksp22I TGATCA 2 cut(s) 328, 586
LpnPI CCDG 8 cut(s) 69, 83, 317, 383, 817, 893, 985, 1044
Lsp1109I GCAGC 2 cut(s) 493, 1585
LweI GCATC 3 cut(s) 235, 430, 1291
MaeI CTAG 3 cut(s) 629, 797, 1365
MaeIII GTNAC 4 cut(s) 449, 650, 1244, 1291
MboII GAAGA 6 cut(s) 199, 542, 686, 980, 1565, 1588
MfeI CAATTG 2 cut(s) 993, 1413
MflI RGATCY 2 cut(s) 16, 1534
MmeI TCCRAC 2 cut(s) 277, 708
Mph1103I ATGCAT 2 cut(s) 1019, 1306
MroXI GAANNNNTTC 3 cut(s) 63, 1046, 1070
MseI TTAA 3 cut(s) 135, 516, 1520
MslI CAYNNNNRTG 4 cut(s) 312, 342, 675, 1309
MspCI CTTAAG 1 cut(s) 515
MspI CCGG 1 cut(s) 1031
MspR9I CCNGG 1 cut(s) 1031
MunI CAATTG 2 cut(s) 993, 1413
MvnI CGCG 1 cut(s) 1509
MwoI GCNNNNNNNGC 3 cut(s) 1136, 1203, 1374
NciI CCSGG 1 cut(s) 1031
NdeI CATATG 1 cut(s) 955
NlaIII CATG 8 cut(s) 317, 329, 658, 674, 1058, 1282, 1358, 1449
NmeAIII GCCGAG 1 cut(s) 1254
NmuCI GTSAC 1 cut(s) 650
NsiI ATGCAT 2 cut(s) 1019, 1306
NspI RCATGY 2 cut(s) 658, 1358
OliI CACNNNNGTG 1 cut(s) 342
PaeI GCATGC 1 cut(s) 1358
PagI TCATGA 2 cut(s) 325, 1054
PciI ACATGT 1 cut(s) 654
PdmI GAANNNNTTC 3 cut(s) 63, 1046, 1070
PfeI GAWTC 4 cut(s) 122, 1066, 1442, 1560
PfoI TCCNGGA 1 cut(s) 1029
PkrI GCNGC 2 cut(s) 508, 1600
PscI ACATGT 1 cut(s) 654
PsiI TTATAA 1 cut(s) 1545
PspPI GGNCC 2 cut(s) 145, 1174
PstI CTGCAG 1 cut(s) 1603
PsuI RGATCY 2 cut(s) 16, 1534
RsaI GTAC 4 cut(s) 39, 848, 884, 961
RsaNI GTAC 4 cut(s) 38, 847, 883, 960
RseI CAYNNNNRTG 4 cut(s) 312, 342, 675, 1309
SaqAI TTAA 3 cut(s) 135, 516, 1520
SatI GCNGC 2 cut(s) 507, 1599
Sau96I GGNCC 2 cut(s) 145, 1174
ScaI AGTACT 1 cut(s) 884
ScrFI CCNGG 1 cut(s) 1031
SfaNI GCATC 3 cut(s) 235, 430, 1291
SfcI CTRYAG 2 cut(s) 12, 1599
SmiMI CAYNNNNRTG 4 cut(s) 312, 342, 675, 1309
SmlI CTYRAG 4 cut(s) 126, 233, 515, 597
SmoI CTYRAG 4 cut(s) 126, 233, 515, 597
SphI GCATGC 1 cut(s) 1358
SsiI CCGC 2 cut(s) 1482, 1532
SspI AATATT 1 cut(s) 1047
SspMI CTAG 3 cut(s) 629, 797, 1365
StyD4I CCNGG 1 cut(s) 1029
StyI CCWWGG 2 cut(s) 259, 603
TaaI ACNGT 4 cut(s) 344, 931, 1234, 1626
TaqI TCGA 7 cut(s) 165, 243, 382, 474, 572, 1156, 1611
TatI WGTACW 2 cut(s) 846, 882
TfiI GAWTC 4 cut(s) 122, 1066, 1442, 1560
Tru1I TTAA 3 cut(s) 135, 516, 1520
Tru9I TTAA 3 cut(s) 135, 516, 1520
TscAI CASTG 3 cut(s) 306, 1623, 1629
TseFI GTSAC 1 cut(s) 650
TseI GCWGC 2 cut(s) 506, 1598
Tsp45I GTSAC 1 cut(s) 650
TspDTI ATGAA 9 cut(s) 100, 687, 693, 717, 1038, 1071, 1157, 1257, 1397
TspGWI ACGGA 1 cut(s) 540
TspRI CASTG 3 cut(s) 306, 1623, 1629
Vha464I CTTAAG 1 cut(s) 515
XagI CCTNNNNNAGG 1 cut(s) 1544
XapI RAATTY 2 cut(s) 79, 1332
XbaI TCTAGA 1 cut(s) 628
XceI RCATGY 2 cut(s) 658, 1358
XcmI CCANNNNNNNNNTGG 1 cut(s) 887
XmnI GAANNNNTTC 3 cut(s) 63, 1046, 1070
XspI CTAG 3 cut(s) 629, 797, 1365
ZrmI AGTACT 1 cut(s) 884
Zsp2I ATGCAT 2 cut(s) 1019, 1306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.