RchiOBHm_Chr6g0270581

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000053
Physical Location & Seq
Forward (+)
28467994 .. 28470674
2681 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24276

Sequence Viewer

Length: 1695 bp
ATGTCGTCTGTGCCAACTAGTTATGAATCATTTCCAGTACCACAAATTGCAAAGCCTGAAATCATTCGTCGGATAGCAAATTTTCAACCAAGCATTTGGGGAGATCAGTTCATCAACTATGATTCCCAAAAAACTACTGATGCCCTTTGTCAGCAGCAAGTTGGCAAATTGAAAACAGTAGTAAAGAGGGAACTCTTTACTAATGAAGGTGATTTTTCACATCGACTCAAGTTAATTGATGCAATCCAACGACTTGGCATGGCATATCATTTTGAACGCGAAATAGAAGAATCATTGCAACATATCCATGCGACATATCGTGATCGGGATCTGTACCGTGATGATGGCCATCTTTACGATGTTGCCCTATTGTTTCGGCTTCTAAGGCAACATGGATATCAAATTTCATCTGGTATATTCAACAAGTTTAAAGATTCAAATGGTAGCTTCAAAGAATGCTTAATTTCTGATGCCTCCGGTATGCTAAGCCTCTATGAAGCAACACATCTTAGGGTTAATGGAGAAGATATACTTGAAGAGGCTCTTTTGTTCACCACCACTCAACTCAAGTACGTGCTCAAAAGCAATGTAAGCTGTCCACCAGTATTGAAGGAACAAATAACTGAAGCTTTGACGAGACCTCTTCGAAAAACTTTGGAGAGATTATGCGCTAAGCGTTATATTTCAATCTACCAAGATGAAGCCTCACACAGTGAATCTCTATTGAAACTTGCAAAGTTGGATTTCAATCTTGTTCAGTCTTTACACCAAAAGGAGCTCTCTGATATTACTAGGTGGTGGAAAGAACTAGACTTTGAAAGGAAATTGCCTTTTGCAAGAGACAGGGTGGTGGAGGTGTTCTTTTGGATGGTGGGAGTATATTTTGAACCCCAGTACTCTATTGGCAGAATCATTATGACAAAAGTGGGTATCCTCCTAACTATTTTGGATGATATCTATGATGCATATGGTACATTTGAAGAACTCATGATCTTCACTCAAGCAATTGACAGGTGGAATGTCAAATGCATAGATGAACTCCCAGACTATTTGAAAATATTCTATCATGAACTTTTGAATCTTTTCGATGAAATTGACAAAGTGATGTCAAAGGAAGGAAGATCATATCGAACTTCATATGCAATAGAAGCTATGAAAGTTCAGGCTCGATCATTTTTCAACGAGGCCCAATGGTTGCAAGAAGGACGCACTCCGAGCATGGAAGAATATATGAGTGTTGCTACAGTTTCTGTTACTTACCCTTTTATAACAACCATTTCATTACTTGGCATGGGAGATATTGTAACGAAGGATGCATTTGAGTGGTTGTTGGATGACCCGAAAATTGTTAGCGCTTCCAATATCATTTTTAGGCTCATGGATGACATTGTTTCCACCAAGTTTGAGAAAGAGAGAGGGCATGCACCTTCTAGCATTGATTGCTATATGAAGCAATATGGTGTTTCAGAGCAAGAGACGATTGACGTTTTCGGGAAACAAATTGCGAAATCATGGAAGGATATAAATGAGGAGTTTCTTAAACCAACCGCTATGCCAATGCCGGTTCTTATGCGTGTTCTCAATTTAACAAGAGTAGTGGATCTTCTTTACAAGGGAGAAGATGGATTCACACGAGTTGGGAAGATGACCAAAGATAGTGTTGCTGCAGTTATTATCGACTCAGTGCCACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

564

Amino Acids

65.34

Weight (kDa)

5.5

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 32 - 211 3.2e-56 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 268 - 507 2.5e-97 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 308 - 507 5.9e-57 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1268
AccII CGCG 1 cut(s) 279
AciI CCGC 1 cut(s) 1548
AclWI GGATC 2 cut(s) 336, 1608
AcoI YGGCCR 1 cut(s) 346
AcsI RAATTY 2 cut(s) 79, 402
AcuI CTGAAG 1 cut(s) 645
AdeI CACNNNGTG 1 cut(s) 713
AfaI GTAC 5 cut(s) 39, 335, 572, 896, 973
AfeI AGCGCT 1 cut(s) 1354
AhlI ACTAGT 1 cut(s) 17
AluBI AGCT 5 cut(s) 447, 594, 629, 778, 1151
AluI AGCT 5 cut(s) 447, 594, 629, 778, 1151
Alw21I GWGCWC 2 cut(s) 579, 780
Alw26I GTCTC 3 cut(s) 631, 834, 1469
AlwI GGATC 2 cut(s) 336, 1608
AlwNI CAGNNNCTG 1 cut(s) 1250
Aor51HI AGCGCT 1 cut(s) 1354
AoxI GGCC 2 cut(s) 346, 1185
ApeKI GCWGC 2 cut(s) 154, 1664
ApoI RAATTY 2 cut(s) 79, 402
Asp700I GAANNNNTTC 4 cut(s) 30, 63, 1058, 1082
AspLEI GCGC 2 cut(s) 671, 1355
AspS9I GGNCC 1 cut(s) 1186
AsuHPI GGTGA 2 cut(s) 221, 544
AsuII TTCGAA 1 cut(s) 646
BalI TGGCCA 1 cut(s) 348
BanII GRGCYC 1 cut(s) 780
BarI GAAGNNNNNNTAC 2 cut(s) 1587, 1619
BauI CACGAG 1 cut(s) 1632
Bbv12I GWGCWC 2 cut(s) 579, 780
BbvI GCAGC 2 cut(s) 166, 1651
BccI CCATC 4 cut(s) 338, 357, 862, 1616
BciVI GTATCC 1 cut(s) 941
BcoDI GTCTC 3 cut(s) 631, 834, 1469
BcuI ACTAGT 1 cut(s) 17
BfaI CTAG 4 cut(s) 18, 792, 809, 1431
BfmI CTRYAG 2 cut(s) 1242, 1665
BfoI RGCGCY 1 cut(s) 1356
BfuI GTATCC 1 cut(s) 941
BisI GCNGC 2 cut(s) 155, 1665
BlpI GCTNAGC 2 cut(s) 485, 672
BlsI GCNGC 2 cut(s) 156, 1666
BmcAI AGTACT 1 cut(s) 896
BmgT120I GGNCC 1 cut(s) 1186
BmrI ACTGGG 1 cut(s) 886
BmsI GCATC 5 cut(s) 130, 229, 460, 952, 1303
BmuI ACTGGG 1 cut(s) 886
Bpu1102I GCTNAGC 2 cut(s) 485, 672
Bpu14I TTCGAA 1 cut(s) 646
BpuEI CTTGAG 3 cut(s) 212, 551, 984
BsaAI YACGTR 1 cut(s) 574
BsaBI GATNNNNATC 3 cut(s) 327, 348, 747
BsaI GGTCTC 1 cut(s) 631
BsaWI WCCGGW 1 cut(s) 476
BsaXI ACNNNNNCTCC 2 cut(s) 1287, 1317
Bse118I RCCGGY 1 cut(s) 1561
Bse1I ACTGG 3 cut(s) 35, 602, 892
Bse3DI GCAATG 2 cut(s) 293, 592
Bse8I GATNNNNATC 3 cut(s) 327, 348, 747
BseGI GGATG 5 cut(s) 873, 955, 1318, 1339, 1387
BseJI GATNNNNATC 3 cut(s) 327, 348, 747
BseMI GCAATG 2 cut(s) 293, 592
BseMII CTCAG 1 cut(s) 1695
BseNI ACTGG 3 cut(s) 35, 602, 892
BseRI GAGGAG 1 cut(s) 1544
BseXI GCAGC 2 cut(s) 166, 1651
Bsh1236I CGCG 1 cut(s) 279
BshFI GGCC 2 cut(s) 348, 1187
BsiHKAI GWGCWC 2 cut(s) 579, 780
BsiSI CCGG 2 cut(s) 477, 1562
BsmAI GTCTC 3 cut(s) 631, 834, 1469
BsmBI CGTCTC 1 cut(s) 1469
BsmI GAATGC 1 cut(s) 461
BsnI GGCC 2 cut(s) 348, 1187
Bso31I GGTCTC 1 cut(s) 631
Bsp119I TTCGAA 1 cut(s) 646
Bsp1286I GDGCHC 2 cut(s) 579, 780
Bsp143I GATC 7 cut(s) 103, 322, 328, 990, 1121, 1169, 1600
Bsp1720I GCTNAGC 2 cut(s) 485, 672
BspACI CCGC 1 cut(s) 1548
BspANI GGCC 2 cut(s) 348, 1187
BspCNI CTCAG 1 cut(s) 1694
BspFNI CGCG 1 cut(s) 279
BspHI TCATGA 2 cut(s) 987, 1066
BspMAI CTGCAG 1 cut(s) 1669
BspPI GGATC 2 cut(s) 336, 1608
BspT104I TTCGAA 1 cut(s) 646
BspTNI GGTCTC 1 cut(s) 631
BsrDI GCAATG 2 cut(s) 293, 592
BsrFI RCCGGY 1 cut(s) 1561
BsrI ACTGG 3 cut(s) 35, 602, 892
BssAI RCCGGY 1 cut(s) 1561
BssMI GATC 7 cut(s) 103, 322, 328, 990, 1121, 1169, 1600
BssSI CACGAG 1 cut(s) 1632
Bst2BI CACGAG 1 cut(s) 1632
Bst4CI ACNGT 5 cut(s) 178, 338, 713, 1246, 1692
Bst6I CTCTTC 2 cut(s) 531, 648
BstAPI GCANNNNNTGC 1 cut(s) 1440
BstBAI YACGTR 1 cut(s) 574
BstBI TTCGAA 1 cut(s) 646
BstC8I GCNNGC 1 cut(s) 1422
BstDEI CTNAG 5 cut(s) 383, 485, 509, 672, 1681
BstF5I GGATG 5 cut(s) 873, 955, 1318, 1339, 1387
BstFNI CGCG 1 cut(s) 279
BstH2I RGCGCY 1 cut(s) 1356
BstHHI GCGC 2 cut(s) 671, 1355
BstKTI GATC 7 cut(s) 106, 325, 331, 993, 1124, 1172, 1603
BstMAI GTCTC 3 cut(s) 631, 834, 1469
BstMBI GATC 7 cut(s) 103, 322, 328, 990, 1121, 1169, 1600
BstMWI GCNNNNNNNGC 5 cut(s) 385, 591, 1148, 1215, 1440
BstNSI RCATGY 1 cut(s) 1424
BstSFI CTRYAG 2 cut(s) 1242, 1665
BstUI CGCG 1 cut(s) 279
BstV1I GCAGC 2 cut(s) 166, 1651
BstX2I RGATCY 2 cut(s) 328, 1600
BstXI CCANNNNNNTGG 2 cut(s) 96, 254
BstYI RGATCY 2 cut(s) 328, 1600
BsuI GTATCC 1 cut(s) 941
BsuRI GGCC 2 cut(s) 348, 1187
BtsCI GGATG 5 cut(s) 873, 955, 1318, 1339, 1387
BtsIMutI CAGTG 3 cut(s) 718, 1688, 1689
Cac8I GCNNGC 1 cut(s) 1422
CaiI CAGNNNCTG 1 cut(s) 1250
CciI TCATGA 2 cut(s) 987, 1066
CfoI GCGC 2 cut(s) 671, 1355
Cfr10I RCCGGY 1 cut(s) 1561
Cfr13I GGNCC 1 cut(s) 1186
CseI GACGC 1 cut(s) 1215
Csp6I GTAC 5 cut(s) 38, 334, 571, 895, 972
CspCI CAANNNNNGTGG 6 cut(s) 588, 623, 1384, 1419, 1578, 1613
CviQI GTAC 5 cut(s) 38, 334, 571, 895, 972
DdeI CTNAG 5 cut(s) 383, 485, 509, 672, 1681
DpnI GATC 7 cut(s) 105, 324, 330, 992, 1123, 1171, 1602
DpnII GATC 7 cut(s) 103, 322, 328, 990, 1121, 1169, 1600
DraI TTTAAA 1 cut(s) 430
DraIII CACNNNGTG 1 cut(s) 713
EaeI YGGCCR 1 cut(s) 346
Eam1104I CTCTTC 2 cut(s) 531, 648
EarI CTCTTC 2 cut(s) 531, 648
Ecl136II GAGCTC 1 cut(s) 778
Eco24I GRGCYC 1 cut(s) 780
Eco31I GGTCTC 1 cut(s) 631
Eco32I GATATC 2 cut(s) 398, 955
Eco47III AGCGCT 1 cut(s) 1354
Eco53kI GAGCTC 1 cut(s) 778
Eco57I CTGAAG 1 cut(s) 645
EcoICRI GAGCTC 1 cut(s) 778
EcoRV GATATC 2 cut(s) 398, 955
EcoT22I ATGCAT 3 cut(s) 967, 1031, 1318
EcoT38I GRGCYC 1 cut(s) 780
Esp3I CGTCTC 1 cut(s) 1469
FalI AAGNNNNNCTT 4 cut(s) 516, 548, 528, 560
FauNDI CATATG 2 cut(s) 967, 1138
Fnu4HI GCNGC 2 cut(s) 155, 1665
FokI GGATG 5 cut(s) 880, 962, 1325, 1346, 1394
FriOI GRGCYC 1 cut(s) 780
Fsp4HI GCNGC 2 cut(s) 155, 1665
FspBI CTAG 4 cut(s) 18, 792, 809, 1431
GlaI GCGC 2 cut(s) 670, 1354
GluI GCNGC 2 cut(s) 155, 1665
HaeII RGCGCY 1 cut(s) 1356
HaeIII GGCC 2 cut(s) 348, 1187
HapII CCGG 2 cut(s) 477, 1562
HgaI GACGC 1 cut(s) 1215
HhaI GCGC 2 cut(s) 671, 1355
Hin6I GCGC 2 cut(s) 669, 1353
HinP1I GCGC 2 cut(s) 669, 1353
HindIII AAGCTT 1 cut(s) 627
HpaII CCGG 2 cut(s) 477, 1562
HphI GGTGA 2 cut(s) 221, 544
Hpy166II GTNNAC 2 cut(s) 552, 599
Hpy188I TCNGA 5 cut(s) 72, 469, 784, 1215, 1468
Hpy188III TCNNGA 5 cut(s) 320, 326, 988, 1067, 1492
Hpy8I GTNNAC 2 cut(s) 552, 599
Hpy99I CGWCG 1 cut(s) 72
HpyAV CCTTC 7 cut(s) 200, 604, 1109, 1196, 1303, 1437, 1510
HpyCH4III ACNGT 5 cut(s) 178, 338, 713, 1246, 1692
HpyCH4IV ACGT 2 cut(s) 573, 1485
HpyF10VI GCNNNNNNNGC 5 cut(s) 385, 591, 1148, 1215, 1440
HpyF3I CTNAG 5 cut(s) 383, 485, 509, 672, 1681
HpySE526I ACGT 2 cut(s) 573, 1485
HspAI GCGC 2 cut(s) 669, 1353
Kzo9I GATC 7 cut(s) 103, 322, 328, 990, 1121, 1169, 1600
LmnI GCTCC 1 cut(s) 775
Lsp1109I GCAGC 2 cut(s) 166, 1651
LweI GCATC 5 cut(s) 130, 229, 460, 952, 1303
MaeI CTAG 4 cut(s) 18, 792, 809, 1431
MaeII ACGT 2 cut(s) 573, 1485
MaeIII GTNAC 2 cut(s) 1252, 1303
MalI GATC 7 cut(s) 105, 324, 330, 992, 1123, 1171, 1602
MboI GATC 7 cut(s) 103, 322, 328, 990, 1121, 1169, 1600
MfeI CAATTG 1 cut(s) 1005
MflI RGATCY 2 cut(s) 328, 1600
MhlI GDGCHC 2 cut(s) 579, 780
MlsI TGGCCA 1 cut(s) 348
MluNI TGGCCA 1 cut(s) 348
MlyI GAGTC 2 cut(s) 219, 1673
MmeI TCCRAC 4 cut(s) 50, 271, 720, 1311
Mox20I TGGCCA 1 cut(s) 348
Mph1103I ATGCAT 3 cut(s) 967, 1031, 1318
MroXI GAANNNNTTC 4 cut(s) 30, 63, 1058, 1082
MscI TGGCCA 1 cut(s) 348
MseI TTAA 6 cut(s) 233, 429, 461, 516, 1539, 1586
MslI CAYNNNNRTG 2 cut(s) 306, 1321
Msp20I TGGCCA 1 cut(s) 348
MspI CCGG 2 cut(s) 477, 1562
MunI CAATTG 1 cut(s) 1005
Mva1269I GAATGC 1 cut(s) 461
MvnI CGCG 1 cut(s) 279
MwoI GCNNNNNNNGC 5 cut(s) 385, 591, 1148, 1215, 1440
NdeI CATATG 2 cut(s) 967, 1138
NdeII GATC 7 cut(s) 103, 322, 328, 990, 1121, 1169, 1600
NsiI ATGCAT 3 cut(s) 967, 1031, 1318
NspI RCATGY 1 cut(s) 1424
NspV TTCGAA 1 cut(s) 646
PaeI GCATGC 1 cut(s) 1424
PagI TCATGA 2 cut(s) 987, 1066
PctI GAATGC 1 cut(s) 461
PdmI GAANNNNTTC 4 cut(s) 30, 63, 1058, 1082
PfeI GAWTC 8 cut(s) 26, 122, 290, 434, 716, 909, 1078, 1626
PkrI GCNGC 2 cut(s) 156, 1666
PleI GAGTC 2 cut(s) 219, 1673
PpsI GAGTC 2 cut(s) 219, 1673
Ppu21I YACGTR 1 cut(s) 574
PsiI TTATAA 1 cut(s) 1268
Psp124BI GAGCTC 1 cut(s) 780
PspPI GGNCC 1 cut(s) 1186
PstI CTGCAG 1 cut(s) 1669
PstNI CAGNNNCTG 1 cut(s) 1250
PsuI RGATCY 2 cut(s) 328, 1600
RsaI GTAC 5 cut(s) 39, 335, 572, 896, 973
RsaNI GTAC 5 cut(s) 38, 334, 571, 895, 972
RseI CAYNNNNRTG 2 cut(s) 306, 1321
SacI GAGCTC 1 cut(s) 780
SaqAI TTAA 6 cut(s) 233, 429, 461, 516, 1539, 1586
SatI GCNGC 2 cut(s) 155, 1665
Sau3AI GATC 7 cut(s) 103, 322, 328, 990, 1121, 1169, 1600
Sau96I GGNCC 1 cut(s) 1186
ScaI AGTACT 1 cut(s) 896
SchI GAGTC 2 cut(s) 219, 1673
SduI GDGCHC 2 cut(s) 579, 780
SfaNI GCATC 5 cut(s) 130, 229, 460, 952, 1303
SfcI CTRYAG 2 cut(s) 1242, 1665
SfuI TTCGAA 1 cut(s) 646
SmiMI CAYNNNNRTG 2 cut(s) 306, 1321
SmlI CTYRAG 3 cut(s) 227, 566, 999
SmoI CTYRAG 3 cut(s) 227, 566, 999
SpeI ACTAGT 1 cut(s) 17
SphI GCATGC 1 cut(s) 1424
SsiI CCGC 1 cut(s) 1548
SspI AATATT 1 cut(s) 1059
SspMI CTAG 4 cut(s) 18, 792, 809, 1431
SstI GAGCTC 1 cut(s) 780
TaaI ACNGT 5 cut(s) 178, 338, 713, 1246, 1692
TaiI ACGT 2 cut(s) 576, 1488
TaqI TCGA 6 cut(s) 223, 646, 1086, 1129, 1168, 1677
TatI WGTACW 1 cut(s) 894
TfiI GAWTC 8 cut(s) 26, 122, 290, 434, 716, 909, 1078, 1626
Tru1I TTAA 6 cut(s) 233, 429, 461, 516, 1539, 1586
Tru9I TTAA 6 cut(s) 233, 429, 461, 516, 1539, 1586
TscAI CASTG 3 cut(s) 718, 1689, 1695
TseI GCWGC 2 cut(s) 154, 1664
TspRI CASTG 3 cut(s) 718, 1689, 1695
XapI RAATTY 2 cut(s) 79, 402
XceI RCATGY 1 cut(s) 1424
XcmI CCANNNNNNNNNTGG 1 cut(s) 899
XmnI GAANNNNTTC 4 cut(s) 30, 63, 1058, 1082
XspI CTAG 4 cut(s) 18, 792, 809, 1431
ZrmI AGTACT 1 cut(s) 896
Zsp2I ATGCAT 3 cut(s) 967, 1031, 1318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.