Rw5G036560

(-)-germacrene D synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000053
Physical Location & Seq
Reverse (-)
64116901 .. 64120388
3488 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G036560.1

Sequence Viewer

Length: 1671 bp
ATGTCTTCACAAGTTTTAGCATCTCAATCTCAAGCTCCATATACCAGACCTTCAGCTAATTACCCTCCAAGCATTTGGGGTGATCATTTTCTCTCTTATGCTAAGGCGGAAGTGGACTTTAATCTCAAGCAACGAGTCCAAGAACTTAAAAGAGAAGTGAAAATGATCCTCATGGTGCCAGCAAAAGAGCCTTCACAAAAATTGAACTTAGTTGATGACATTCAGCGCCTAGGCGTATCTTACCATTTTGAAAATGAGATCGATGAAATTCTGAAACAAATTTACCAGACTACTTATGGTAGTGATGTGGAATATGATGATGACCTTTACACTACTGCTCTTTGTTTTCGGCTACTCAGACAACACGGTTATAATGTTTCAAGTGATATGTTCAACAAGTACTTCAAGGAGGGTAATGATGAGAAATTTAAGGAATCTCTTTTGAGTGATGTATCAGGACTATTAAGTTTATACGAAGCCACCCATCTTAGGATACACGGTGAAGACATACTCGAAGAGGCACTAGCCTTCACCACCACACATCTCGAGTCGATGAAAGACAGTTTAAGCCCTCCACTTTTGAAACAAGTAGCCCATTCCTTAAATCAGCCACTACATAAGGGCATCACTAGGGTGGAAGCAAGATATTACTTATCAATCTACCACGAATGCAATTCACATAATGAAACTCTCCTGACTTTTGCGAAGTTGGATTTCAACCTGTTGCAGCAGCTCCATCAAAACGAACTTTGTGAAATTACAAGGTGGTGGAAGGACTTGGACGTGAGAAACGAACTGCTGTTTACAAGAGACAGAGTTACTGAGGTTTACTTCTGCTGGGCTTCATCAGTCTGCTTTGAACCTGAATATTCCTTTGCTAGGAGGACATTATGCAAAGTTACTGCCCTGGCATCCATTCTCGATGACATTTATGATTTGTATGGCACATTCGAAGAACTAGAGCTCTTTACTGAAGCTATTGAGAGGTGGGATATATGTTTCATGGATGCACTGCCAAACTATATGAAAGTCTATTATAAGGCATTGTTAGACGTGTATACTGAAGTTGAAGAAGAGCTTGCAAAGGAGGGAAAGTTGTATCGAATCCATTATGCCATAGAAGCTATGAAAAAGCTAGCTCAATGTTACTTTCTTGAAGCGAAATGGTTCCACCAGAAACATGTACCAACAGTGGATGAGTATATTGCCCTATCATCACTGACGTCAGGCTACCCACTGCTTGTGACCACCTCTTTTGTTACAATGGGAGATATTGCTACACAAGATTCCTTTGACTGGTTGGCCACTTATCCCAAACCTGTGAAAGGGTGTGCAATAGTTGCCCGACTTATGGATGACTTGGTGTCTCACAAGTTTGAGCAAAAAAGAGGACATGTTGCGTCAGCTGTAGAGTGCTACATGAAAGAATTTGGTGCCACAGAAGAAGAAGCAATAATTGCAATGCGTAGAAAAGTGAGTGGTGCATGGAAGGACATAAACGAATCGTTCCTCTTCCCCAATGCCATTTCAAGGCCACTGTTGACTCGAATTCTTAATTTAGCATGCGTCATGGATGTTGTCTACAAGAATGAAGATGGTTACACTACTGATCAGCATGGTATACTCAAGGATCTTATAACTTCTACGCTTATCCAACAAGTGCCTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

556

Amino Acids

64.35

Weight (kDa)

5.19

Isoelectric Point (pI)

45.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 25 - 201 2e-60 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 258 - 498 6e-91 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 299 - 498 8.3e-61 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 372, 1038, 1637
AatII GACGTC 1 cut(s) 1226
AccB1I GGYRCC 2 cut(s) 175, 1433
AccI GTMKAC 3 cut(s) 1058, 1581, 1621
AciI CCGC 1 cut(s) 107
AclWI GGATC 2 cut(s) 160, 1638
AcoI YGGCCR 1 cut(s) 1302
AcsI RAATTY 5 cut(s) 267, 279, 425, 1427, 1548
AcuI CTGAAG 3 cut(s) 36, 993, 1083
AcyI GRCGYC 1 cut(s) 1223
AfaI GTAC 2 cut(s) 401, 1185
AfiI CCNNNNNNNGG 6 cut(s) 489, 879, 1296, 1325, 1351, 1530
AflIII ACRYGT 3 cut(s) 1053, 1180, 1393
AhdI GACNNNNNGTC 1 cut(s) 1363
AjiI CACGTC 2 cut(s) 784, 1054
AjnI CCWGG 1 cut(s) 906
AleI CACNNNNGTG 1 cut(s) 632
Alw21I GWGCWC 1 cut(s) 966
Alw26I GTCTC 2 cut(s) 804, 1371
AlwI GGATC 2 cut(s) 160, 1638
Ama87I CYCGRG 1 cut(s) 545
AoxI GGCC 2 cut(s) 1302, 1532
ApeKI GCWGC 2 cut(s) 727, 730
ApoI RAATTY 5 cut(s) 267, 279, 425, 1427, 1548
Asp700I GAANNNNTTC 1 cut(s) 1166
AspA2I CCTAGG 1 cut(s) 229
AspLEI GCGC 1 cut(s) 228
AsuHPI GGTGA 3 cut(s) 92, 512, 523
AsuII TTCGAA 1 cut(s) 951
AsuNHI GCTAGC 1 cut(s) 1135
AvaI CYCGRG 1 cut(s) 545
AvrII CCTAGG 1 cut(s) 229
BalI TGGCCA 1 cut(s) 1304
BanI GGYRCC 2 cut(s) 175, 1433
BanII GRGCYC 1 cut(s) 966
BbsI GAAGAC 1 cut(s) 510
Bbv12I GWGCWC 1 cut(s) 966
BbvI GCAGC 2 cut(s) 739, 742
BccI CCATC 3 cut(s) 492, 744, 1589
BciT130I CCWGG 1 cut(s) 908
BciVI GTATCC 1 cut(s) 486
BclI TGATCA 2 cut(s) 82, 1609
BcoDI GTCTC 2 cut(s) 804, 1371
BfaI CTAG 6 cut(s) 230, 524, 630, 879, 959, 1136
BfmI CTRYAG 1 cut(s) 1407
BfoI RGCGCY 1 cut(s) 229
BfuI GTATCC 1 cut(s) 486
BisI GCNGC 2 cut(s) 728, 731
BlnI CCTAGG 1 cut(s) 229
BlsI GCNGC 2 cut(s) 729, 732
BmcAI AGTACT 1 cut(s) 401
Bme1390I CCNGG 1 cut(s) 908
BmeRI GACNNNNNGTC 1 cut(s) 1363
BmeT110I CYCGRG 1 cut(s) 545
BmgBI CACGTC 2 cut(s) 784, 1054
BmiI GGNNCC 3 cut(s) 177, 1169, 1435
BmrFI CCNGG 1 cut(s) 908
BmsI GCATC 4 cut(s) 29, 633, 920, 997
BmtI GCTAGC 1 cut(s) 1139
BpiI GAAGAC 1 cut(s) 510
Bpu10I CCTNAGC 1 cut(s) 102
Bpu14I TTCGAA 1 cut(s) 951
BpuEI CTTGAG 3 cut(s) 15, 110, 1610
Bsa29I ATCGAT 1 cut(s) 261
BsaHI GRCGYC 1 cut(s) 1223
BsaJI CCNNGG 2 cut(s) 229, 906
BsaXI ACNNNNNCTCC 2 cut(s) 1079, 1109
Bsc4I CCNNNNNNNGG 6 cut(s) 489, 879, 1296, 1325, 1351, 1530
Bse1I ACTGG 1 cut(s) 1301
Bse3DI GCAATG 1 cut(s) 1467
BseBI CCWGG 1 cut(s) 908
BseCI ATCGAT 1 cut(s) 261
BseDI CCNNGG 2 cut(s) 229, 906
BseGI GGATG 5 cut(s) 911, 1012, 1201, 1360, 1579
BseLI CCNNNNNNNGG 6 cut(s) 489, 879, 1296, 1325, 1351, 1530
BseMI GCAATG 1 cut(s) 1467
BseMII CTCAG 2 cut(s) 370, 813
BseNI ACTGG 1 cut(s) 1301
BseXI GCAGC 2 cut(s) 739, 742
BseYI CCCAGC 1 cut(s) 837
BshFI GGCC 2 cut(s) 1304, 1534
BshNI GGYRCC 2 cut(s) 175, 1433
BshVI ATCGAT 1 cut(s) 261
BsiHKAI GWGCWC 1 cut(s) 966
BsiHKCI CYCGRG 1 cut(s) 545
BslI CCNNNNNNNGG 6 cut(s) 489, 879, 1296, 1325, 1351, 1530
BsmAI GTCTC 2 cut(s) 804, 1371
BsmI GAATGC 1 cut(s) 674
BsnI GGCC 2 cut(s) 1304, 1534
BsoBI CYCGRG 1 cut(s) 545
Bsp119I TTCGAA 1 cut(s) 951
Bsp1286I GDGCHC 1 cut(s) 966
Bsp143I GATC 5 cut(s) 82, 165, 258, 1609, 1630
BspACI CCGC 1 cut(s) 107
BspANI GGCC 2 cut(s) 1304, 1534
BspCNI CTCAG 2 cut(s) 369, 814
BspDI ATCGAT 1 cut(s) 261
BspLI GGNNCC 3 cut(s) 177, 1169, 1435
BspOI GCTAGC 1 cut(s) 1139
BspPI GGATC 2 cut(s) 160, 1638
BspQI GCTCTTC 1 cut(s) 1068
BspT104I TTCGAA 1 cut(s) 951
BspT107I GGYRCC 2 cut(s) 175, 1433
BsrDI GCAATG 1 cut(s) 1467
BsrI ACTGG 1 cut(s) 1301
BssECI CCNNGG 2 cut(s) 229, 906
BssMI GATC 5 cut(s) 82, 165, 258, 1609, 1630
BssNAI GTATAC 2 cut(s) 1059, 1622
BssNI GRCGYC 1 cut(s) 1223
BssT1I CCWWGG 1 cut(s) 229
Bst1107I GTATAC 2 cut(s) 1059, 1622
Bst2UI CCWGG 1 cut(s) 908
Bst4CI ACNGT 5 cut(s) 368, 500, 563, 1192, 1539
Bst6I CTCTTC 3 cut(s) 510, 1068, 1517
BstACI GRCGYC 1 cut(s) 1223
BstAPI GCANNNNNTGC 2 cut(s) 1340, 1457
BstBI TTCGAA 1 cut(s) 951
BstC8I GCNNGC 4 cut(s) 180, 1080, 1137, 1564
BstDEI CTNAG 5 cut(s) 102, 208, 356, 488, 822
BstENI CCTNNNNNAGG 2 cut(s) 877, 1323
BstF5I GGATG 5 cut(s) 911, 1012, 1201, 1360, 1579
BstH2I RGCGCY 1 cut(s) 229
BstHHI GCGC 1 cut(s) 228
BstKTI GATC 5 cut(s) 85, 168, 261, 1612, 1633
BstMAI GTCTC 2 cut(s) 804, 1371
BstMBI GATC 5 cut(s) 82, 165, 258, 1609, 1630
BstMWI GCNNNNNNNGC 3 cut(s) 1121, 1340, 1457
BstNI CCWGG 1 cut(s) 908
BstNSI RCATGY 3 cut(s) 1184, 1397, 1566
BstSCI CCNGG 1 cut(s) 906
BstSFI CTRYAG 1 cut(s) 1407
BstV1I GCAGC 2 cut(s) 739, 742
BstV2I GAAGAC 1 cut(s) 510
BstX2I RGATCY 1 cut(s) 1630
BstXI CCANNNNNNTGG 1 cut(s) 75
BstYI RGATCY 1 cut(s) 1630
BstZ17I GTATAC 2 cut(s) 1059, 1622
Bsu15I ATCGAT 1 cut(s) 261
BsuI GTATCC 1 cut(s) 486
BsuRI GGCC 2 cut(s) 1304, 1534
BsuTUI ATCGAT 1 cut(s) 261
BtrI CACGTC 2 cut(s) 784, 1054
BtsCI GGATG 5 cut(s) 911, 1012, 1201, 1360, 1579
BtsI GCAGTG 2 cut(s) 1010, 1235
BtsIMutI CAGTG 5 cut(s) 1010, 1197, 1217, 1235, 1535
Cac8I GCNNGC 4 cut(s) 180, 1080, 1137, 1564
CfoI GCGC 1 cut(s) 228
ClaI ATCGAT 1 cut(s) 261
CseI GACGC 2 cut(s) 1389, 1555
Csp6I GTAC 2 cut(s) 400, 1184
CspCI CAANNNNNGTGG 2 cut(s) 1237, 1272
CviAII CATG 9 cut(s) 172, 1003, 1181, 1394, 1420, 1485, 1563, 1570, 1616
CviQI GTAC 2 cut(s) 400, 1184
DdeI CTNAG 5 cut(s) 102, 208, 356, 488, 822
DpnI GATC 5 cut(s) 84, 167, 260, 1611, 1632
DpnII GATC 5 cut(s) 82, 165, 258, 1609, 1630
DriI GACNNNNNGTC 1 cut(s) 1363
EaeI YGGCCR 1 cut(s) 1302
Eam1104I CTCTTC 3 cut(s) 510, 1068, 1517
Eam1105I GACNNNNNGTC 1 cut(s) 1363
EarI CTCTTC 3 cut(s) 510, 1068, 1517
EciI GGCGGA 1 cut(s) 122
Ecl136II GAGCTC 1 cut(s) 964
Eco130I CCWWGG 1 cut(s) 229
Eco24I GRGCYC 1 cut(s) 966
Eco53kI GAGCTC 1 cut(s) 964
Eco57I CTGAAG 3 cut(s) 36, 993, 1083
Eco88I CYCGRG 1 cut(s) 545
EcoICRI GAGCTC 1 cut(s) 964
EcoNI CCTNNNNNAGG 2 cut(s) 877, 1323
EcoRI GAATTC 1 cut(s) 1548
EcoRII CCWGG 1 cut(s) 906
EcoT14I CCWWGG 1 cut(s) 229
EcoT38I GRGCYC 1 cut(s) 966
ErhI CCWWGG 1 cut(s) 229
FaeI CATG 9 cut(s) 175, 1006, 1184, 1397, 1423, 1488, 1566, 1573, 1619
FalI AAGNNNNNCTT 2 cut(s) 1062, 1094
FatI CATG 9 cut(s) 171, 1002, 1180, 1393, 1419, 1484, 1562, 1569, 1615
FbaI TGATCA 2 cut(s) 82, 1609
FblI GTMKAC 3 cut(s) 1058, 1581, 1621
Fnu4HI GCNGC 2 cut(s) 728, 731
FokI GGATG 5 cut(s) 898, 1019, 1208, 1367, 1586
FriOI GRGCYC 1 cut(s) 966
Fsp4HI GCNGC 2 cut(s) 728, 731
FspBI CTAG 6 cut(s) 230, 524, 630, 879, 959, 1136
GlaI GCGC 1 cut(s) 227
GluI GCNGC 2 cut(s) 728, 731
GsaI CCCAGC 1 cut(s) 841
HaeII RGCGCY 1 cut(s) 229
HaeIII GGCC 2 cut(s) 1304, 1534
HgaI GACGC 2 cut(s) 1389, 1555
HhaI GCGC 1 cut(s) 228
Hin1I GRCGYC 1 cut(s) 1223
Hin1II CATG 9 cut(s) 175, 1006, 1184, 1397, 1423, 1488, 1566, 1573, 1619
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HincII GTYRAC 1 cut(s) 1542
HindII GTYRAC 1 cut(s) 1542
HinfI GANTC 7 cut(s) 135, 434, 548, 1104, 1286, 1502, 1543
HphI GGTGA 3 cut(s) 92, 512, 523
Hpy166II GTNNAC 7 cut(s) 115, 804, 829, 1059, 1542, 1582, 1622
Hpy188I TCNGA 2 cut(s) 273, 359
Hpy188III TCNNGA 5 cut(s) 456, 545, 694, 920, 1154
Hpy8I GTNNAC 7 cut(s) 115, 804, 829, 1059, 1542, 1582, 1622
HpyAV CCTTC 5 cut(s) 60, 201, 538, 766, 1483
HpyCH4III ACNGT 5 cut(s) 368, 500, 563, 1192, 1539
HpyCH4IV ACGT 3 cut(s) 783, 1053, 1223
HpyCH4V TGCA 8 cut(s) 672, 727, 894, 1010, 1082, 1334, 1460, 1484
HpyF10VI GCNNNNNNNGC 3 cut(s) 1121, 1340, 1457
HpyF3I CTNAG 5 cut(s) 102, 208, 356, 488, 822
HpySE526I ACGT 3 cut(s) 783, 1053, 1223
Hsp92I GRCGYC 1 cut(s) 1223
Hsp92II CATG 9 cut(s) 175, 1006, 1184, 1397, 1423, 1488, 1566, 1573, 1619
HspAI GCGC 1 cut(s) 226
Ksp22I TGATCA 2 cut(s) 82, 1609
Kzo9I GATC 5 cut(s) 82, 165, 258, 1609, 1630
LguI GCTCTTC 1 cut(s) 1068
LmnI GCTCC 2 cut(s) 40, 738
Lsp1109I GCAGC 2 cut(s) 739, 742
LweI GCATC 4 cut(s) 29, 633, 920, 997
MaeI CTAG 6 cut(s) 230, 524, 630, 879, 959, 1136
MaeII ACGT 3 cut(s) 783, 1053, 1223
MaeIII GTNAC 6 cut(s) 817, 898, 1145, 1243, 1258, 1598
MalI GATC 5 cut(s) 84, 167, 260, 1611, 1632
MboI GATC 5 cut(s) 82, 165, 258, 1609, 1630
MboII GAAGA 9 cut(s) 515, 527, 965, 1082, 1085, 1454, 1457, 1504, 1604
MflI RGATCY 1 cut(s) 1630
MhlI GDGCHC 1 cut(s) 966
MlsI TGGCCA 1 cut(s) 1304
MluNI TGGCCA 1 cut(s) 1304
MlyI GAGTC 3 cut(s) 144, 557, 1537
MmeI TCCRAC 1 cut(s) 690
Mox20I TGGCCA 1 cut(s) 1304
MroXI GAANNNNTTC 1 cut(s) 1166
MscI TGGCCA 1 cut(s) 1304
MseI TTAA 7 cut(s) 120, 147, 429, 464, 566, 602, 1554
MslI CAYNNNNRTG 1 cut(s) 632
Msp20I TGGCCA 1 cut(s) 1304
MspA1I CMGCKG 1 cut(s) 1406
MspR9I CCNGG 1 cut(s) 908
Mva1269I GAATGC 1 cut(s) 674
MvaI CCWGG 1 cut(s) 908
MwoI GCNNNNNNNGC 3 cut(s) 1121, 1340, 1457
NdeII GATC 5 cut(s) 82, 165, 258, 1609, 1630
NheI GCTAGC 1 cut(s) 1135
NlaIII CATG 9 cut(s) 175, 1006, 1184, 1397, 1423, 1488, 1566, 1573, 1619
NlaIV GGNNCC 3 cut(s) 177, 1169, 1435
NmuCI GTSAC 1 cut(s) 1243
NspI RCATGY 3 cut(s) 1184, 1397, 1566
NspV TTCGAA 1 cut(s) 951
OliI CACNNNNGTG 1 cut(s) 632
PaeI GCATGC 1 cut(s) 1566
PaeR7I CTCGAG 1 cut(s) 545
PciI ACATGT 2 cut(s) 1180, 1393
PciSI GCTCTTC 1 cut(s) 1068
PcsI WCGNNNNNNNCGW 1 cut(s) 789
PctI GAATGC 1 cut(s) 674
PdmI GAANNNNTTC 1 cut(s) 1166
PfeI GAWTC 4 cut(s) 434, 1104, 1286, 1502
PkrI GCNGC 2 cut(s) 729, 732
PleI GAGTC 3 cut(s) 143, 556, 1537
PpsI GAGTC 3 cut(s) 143, 556, 1537
PscI ACATGT 2 cut(s) 1180, 1393
PsiI TTATAA 3 cut(s) 372, 1038, 1637
Psp124BI GAGCTC 1 cut(s) 966
Psp6I CCWGG 1 cut(s) 906
PspFI CCCAGC 1 cut(s) 837
PspGI CCWGG 1 cut(s) 906
PspN4I GGNNCC 3 cut(s) 177, 1169, 1435
PsuI RGATCY 1 cut(s) 1630
PvuII CAGCTG 1 cut(s) 1406
RsaI GTAC 2 cut(s) 401, 1185
RsaNI GTAC 2 cut(s) 400, 1184
RseI CAYNNNNRTG 1 cut(s) 632
SacI GAGCTC 1 cut(s) 966
SapI GCTCTTC 1 cut(s) 1068
SaqAI TTAA 7 cut(s) 120, 147, 429, 464, 566, 602, 1554
SatI GCNGC 2 cut(s) 728, 731
Sau3AI GATC 5 cut(s) 82, 165, 258, 1609, 1630
ScaI AGTACT 1 cut(s) 401
SchI GAGTC 3 cut(s) 144, 557, 1537
ScrFI CCNGG 1 cut(s) 908
SduI GDGCHC 1 cut(s) 966
SfaNI GCATC 4 cut(s) 29, 633, 920, 997
SfcI CTRYAG 1 cut(s) 1407
Sfr274I CTCGAG 1 cut(s) 545
SfuI TTCGAA 1 cut(s) 951
SlaI CTCGAG 1 cut(s) 545
SmiMI CAYNNNNRTG 1 cut(s) 632
SmlI CTYRAG 4 cut(s) 30, 125, 545, 1625
SmoI CTYRAG 4 cut(s) 30, 125, 545, 1625
SphI GCATGC 1 cut(s) 1566
SsiI CCGC 1 cut(s) 107
SspI AATATT 1 cut(s) 869
SspMI CTAG 6 cut(s) 230, 524, 630, 879, 959, 1136
SstI GAGCTC 1 cut(s) 966
StyD4I CCNGG 1 cut(s) 906
StyI CCWWGG 1 cut(s) 229
TaaI ACNGT 5 cut(s) 368, 500, 563, 1192, 1539
TaiI ACGT 3 cut(s) 786, 1056, 1226
TaqI TCGA 8 cut(s) 261, 513, 546, 551, 921, 951, 1102, 1546
TatI WGTACW 1 cut(s) 399
TfiI GAWTC 4 cut(s) 434, 1104, 1286, 1502
Tru1I TTAA 7 cut(s) 120, 147, 429, 464, 566, 602, 1554
Tru9I TTAA 7 cut(s) 120, 147, 429, 464, 566, 602, 1554
TscAI CASTG 5 cut(s) 1017, 1197, 1224, 1242, 1542
TseFI GTSAC 1 cut(s) 1243
TseI GCWGC 2 cut(s) 727, 730
Tsp45I GTSAC 1 cut(s) 1243
TspDTI ATGAA 9 cut(s) 279, 569, 699, 834, 991, 1040, 1142, 1436, 1605
TspRI CASTG 5 cut(s) 1017, 1197, 1224, 1242, 1542
XagI CCTNNNNNAGG 2 cut(s) 877, 1323
XapI RAATTY 5 cut(s) 267, 279, 425, 1427, 1548
XceI RCATGY 3 cut(s) 1184, 1397, 1566
XcmI CCANNNNNNNNNTGG 1 cut(s) 293
XhoI CTCGAG 1 cut(s) 545
XmaJI CCTAGG 1 cut(s) 229
XmiI GTMKAC 3 cut(s) 1058, 1581, 1621
XmnI GAANNNNTTC 1 cut(s) 1166
XspI CTAG 6 cut(s) 230, 524, 630, 879, 959, 1136
ZraI GACGTC 1 cut(s) 1224
ZrmI AGTACT 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.