FvH4_1g05400

(-)-alpha-pinene synthase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000053
Physical Location & Seq
Reverse (-)
2856727 .. 2859478
2752 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g05400.t1

Sequence Viewer

Length: 1674 bp
ATGCCTGTCCAAGCTACTCCAGCAGCTGAATGCCAGATCATCTCTAAGCCGGAAGTTGTTCGGCGCACAGCAAACTTTAAACCTAGCGTTTGGGGAGATCGGTTTACTAACTATGCCGAAGACATTAGAACTCAAGCTCAAATGCAAGAACAAGTTGAGGAGCTGAAACAAGTAGTGAGGAAGGAAGTATTCACTGATGCTGCTGCTGATTCTTCACGTCAACTGAAGCTAATTGCTGCAATCCAGCGCCTCGGTGTGGCTTACCATTTTGAAACTGAAATAGAACAAGCCCTGGAACGTATACATGCGACATATCAAGATATTCATGATGATGATGGTGATCTGTACAATGTTGCTCTTCGTTTTCGGCTACTCAGGCGACATGGATATAATGTTTCCTGCGATGTATTCAACAAGTTCAAAGATACTAATGGTGACTTCAAGAAAAGCTTAGTCACTGATGTGTCTGGTATGCTATGCTTTTATGAGGCGGCCCATCTGAGGGTGCATGGAGAAACATTACTTGAAGAGGCTCTGGTTTTTACCACCACTCATCTCGAGTCAGCAAGTGCAATAAGCTCTTTGCTGAAAACACCAATAACTGAAGCCCTAGAGAGACCTCTACTAAAAACTATGGAGAGGTTAGGTGCTCGGCGTTACATGTCAATATATCAAGATGAAGCTTCACACAGTGAAAATTTACTGAAACTTGCAAAATTAGATTTTAATGTTGTTCAGTGTTTACACAAAAAGGAACTCAGTGACATTCTAAGATGGTATAAGGAACTGGACTTTGCAAGGAGGATGCCTTTTGCACGAGATAGGGTGGTGGAGTTGTTCTTTTGGATAGCGGGAATATATTTTGAACCTCAATACGTCTTTGGGAGACACATTCTCACGAAGCTTATTGAGATAGTAACAGTAATGGATGACATGTATGATGCATTTGGTACATTCGAAGAACTCGTCATCTTGACTGAAGCAATTGACAGGTGGGATGCAAGTTGCATGGATCAACTGCCAGACTATATGCAACCATTTTATATAACACTTCTGGATGTTATCGATGAAGTTGAAGAGGAGCTGACAAAGCAAGGAAGATCTTACCGAATTCACTACGCAAAAGATATTCTGAAGAATCAAGCCAGGCTCTACTTCGCTGAGGCCAGATGGTTCCACGAAGGATGCACCCCAAAAATGGATGAGTATATGCGAGTTGCAGCATCTTCTGTCGGTAACACCATGCTTTCCGTCGTGTCTTTAGTAGGCATGGGAGACATTATAACAAAAGAGGAATTCGAGTGGCTGACCAATGAGCCTAAAATCCTTAGAGCTTCGAATACCATATTTAGGCTTATGGATGACATTGCTGGGTACAAGTTTGAGAAAGAGAGGGGGCATGTTGCTTCTAGTATTGATTGCTACATGAATGAATACGGGGTTTCAGAGCAAGAGACAATTGATATCTTCAACAAACGAATTGTGGATTCGTGGAAGGATATAAACGAAGAGTTTCTGAGACCCACTGCTGCTCCAGTCCCTGTGCTTAATCGTGTTCTTAACCTAACCCGAGTGGTTGATCTGCTTTACAAAAGGGGAGATGCCTTCACGCATGTCGGAAAACTGATGAAAGATTGTATTGCTGCAATGTTTATTGATCCAGTGCCACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00909 map00909
Pfam Domains
Protein Families
Terpene_synth Terpene_synth_C

Protein Analysis

558

Amino Acids

64.47

Weight (kDa)

5.28

Isoelectric Point (pI)

49.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Terpene_synth PF01397 30 - 201 1.8e-57 Terpene synthase, N-terminal domain
Terpene_synth_C PF03936 261 - 500 4.4e-95 Terpene synthase family, metal binding domain
Terpene_syn_C_2 PF19086 301 - 500 1.8e-57 Terpene synthase family 2, C-terminal metal binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000053)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G23960 AT5G23960
fragaria_vesca FvH4_1g04000 FvH4_1g04000 FvH4_1g05400 FvH4_3g01590 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21390 FvH4_3g21391 FvH4_3g21490 FvH4_3g21560 FvH4_3g22162 FvH4_3g32100 FvH4_4g27781 FvH4_4g27790 FvH4_4g27860 FvH4_4g27870 FvH4_4g27940 FvH4_4g27943 FvH4_4g27950 FvH4_4g27951 FvH4_4g27952 FvH4_5g06461 FvH4_5g06470 FvH4_5g06530 FvH4_5g35700 FvH4_5g35711 FvH4_5g35712 FvH4_6g06540 FvH4_6g11440 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_6g19050 FvH4_7g03620 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g13030 FvH4_7g33640 FvH4_7g33640 FvH4_7g33750 FvH4_7g33750
malus_domestica MD00G1215200.v1.1 MD03G1206700.v1.1 MD03G1207000.v1.1 MD03G1214900.v1.1 MD03G1218300.v1.1 MD03G1218400.v1.1 MD07G1269200.v1.1 MD11G1232100.v1.1 MD11G1232700.v1.1 MD11G1236200.v1.1 MD11G1236500.v1.1 MD11G1236600.v1.1 MD12G1162900.v1.1 MD12G1163000.v1.1 MD12G1163400.v1.1 MD12G1163700.v1.1 MD12G1163800.v1.1 MD16G1070800.v1.1
prunus_persica Prupe.4G190700_v2.0.a1 Prupe.4G190800_v2.0.a1 Prupe.4G191000_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194100_v2.0.a1 Prupe.4G194200_v2.0.a1 Prupe.4G194300_v2.0.a1 Prupe.4G194400_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194500_v2.0.a1 Prupe.4G194600_v2.0.a1 Prupe.4G197900_v2.0.a1 Prupe.4G198000_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198200_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198500_v2.0.a1 Prupe.4G198600_v2.0.a1 Prupe.4G198700_v2.0.a1 Prupe.4G199000_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199200_v2.0.a1 Prupe.4G199300_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G199500_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200000_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G200100_v2.0.a1 Prupe.4G238900_v2.0.a1
pyrus_communis pycom03g16780 pycom11g20550 pycom11g20570 pycom11g20780 pycom12g15470 pycom12g15530 pycom12g15590 pycom12g15600
rosa_chinensis RchiOBHm_Chr1g0313881 RchiOBHm_Chr1g0326011 RchiOBHm_Chr1g0326051 RchiOBHm_Chr1g0326061 RchiOBHm_Chr1g0326071 RchiOBHm_Chr1g0326251 RchiOBHm_Chr1g0326311 RchiOBHm_Chr1g0326331 RchiOBHm_Chr1g0326351 RchiOBHm_Chr1g0326391 RchiOBHm_Chr3g0474051 RchiOBHm_Chr3g0474411 RchiOBHm_Chr3g0474441 RchiOBHm_Chr3g0474501 RchiOBHm_Chr3g0474541 RchiOBHm_Chr3g0475221 RchiOBHm_Chr3g0490681 RchiOBHm_Chr4g0418071 RchiOBHm_Chr5g0036921 RchiOBHm_Chr5g0037011 RchiOBHm_Chr5g0037601 RchiOBHm_Chr5g0038021 RchiOBHm_Chr5g0038101 RchiOBHm_Chr5g0044191 RchiOBHm_Chr5g0059501 RchiOBHm_Chr5g0059511 RchiOBHm_Chr5g0059541 RchiOBHm_Chr5g0060241 RchiOBHm_Chr5g0060261 RchiOBHm_Chr5g0060571 RchiOBHm_Chr5g0065101 RchiOBHm_Chr6g0245681 RchiOBHm_Chr6g0245691 RchiOBHm_Chr6g0245751 RchiOBHm_Chr6g0246001 RchiOBHm_Chr6g0246011 RchiOBHm_Chr6g0265741 RchiOBHm_Chr6g0270581 RchiOBHm_Chr6g0274871 RchiOBHm_Chr6g0305391 RchiOBHm_Chr7g0210371 RchiOBHm_Chr7g0212441 RchiOBHm_Chr7g0227831 RchiOBHm_Chr7g0228501
rosa_laevigata RLG00000001556 RLG00000001557 RLG00000001562 RLG00000002890 RLG00000006572 RLG00000008725 RLG00000014163 RLG00000015277 RLG00000017042 RLG00000018206 RLG00000023912 RLG00000023916 RLG00000023920 RLG00000023955 RLG00000028351 RLG00000028821 RLG00000028822 RLG00000028823 RLG00000028824 RLG00000029856 RLG00000030075 RLG00000030076 RLG00000030080 RLG00000033743 RLG00000034250 RLG00000035299 RLG00000035350 RLG00000035387 RLG00000035755
rosa_multiflora Rmu_co8151952.1_g000001 Rmu_co8453003.1_g000001 Rmu_sc0000028.1_g000016 Rmu_sc0000208.1_g000003 Rmu_sc0000535.1_g000005 Rmu_sc0000535.1_g000015 Rmu_sc0000613.1_g000028 Rmu_sc0001217.1_g000009 Rmu_sc0001217.1_g000020 Rmu_sc0001353.1_g000001 Rmu_sc0001353.1_g000030 Rmu_sc0002281.1_g000004 Rmu_sc0002281.1_g000021 Rmu_sc0002670.1_g000004 Rmu_sc0002782.1_g000013 Rmu_sc0002915.1_g000015 Rmu_sc0003032.1_g000026 Rmu_sc0004042.1_g000001 Rmu_sc0004192.1_g000012 Rmu_sc0004192.1_g000022 Rmu_sc0004391.1_g000015 Rmu_sc0004507.1_g000042 Rmu_sc0004507.1_g000044 Rmu_sc0006021.1_g000024 Rmu_sc0006219.1_g000003 Rmu_sc0006435.1_g000010 Rmu_sc0007576.1_g000002 Rmu_sc0011334.1_g000013 Rmu_sc0024896.1_g000001 Rmu_ssc0000062.1_g000038 Rmu_ssc0000410.1_g000031
rosa_roxburghii Rroxscaffold_175G00432520 Rroxscaffold_1G00016230 Rroxscaffold_1G00040400 Rroxscaffold_1G00042840 Rroxscaffold_1G00042960 Rroxscaffold_1G00043940 Rroxscaffold_1G00044030 Rroxscaffold_2G00123010 Rroxscaffold_2G00123030 Rroxscaffold_2G00123040 Rroxscaffold_3G00231080 Rroxscaffold_3G00235280 Rroxscaffold_3G00246840 Rroxscaffold_3G00246850 Rroxscaffold_4G00303170 Rroxscaffold_4G00317190 Rroxscaffold_4G00323790 Rroxscaffold_4G00323810 Rroxscaffold_4G00323850 Rroxscaffold_4G00324000 Rroxscaffold_6G00407090 Rroxscaffold_6G00407140 Rroxscaffold_6G00407170 Rroxscaffold_6G00407190 Rroxscaffold_6G00407540 Rroxscaffold_7G00197730 Rroxscaffold_7G00197820 Rroxscaffold_7G00202480 Rroxscaffold_7G00215030
rosa_rugosa Rorug01G0058200 Rorug01G0058300 Rorug01G0058500 Rorug01G0060200 Rorug01G0225900 Rorug03G0137700 Rorug03G0137800 Rorug03G0137900 Rorug03G0138000 Rorug03G0138100 Rorug03G0140800 Rorug03G0141100 Rorug03G0141200 Rorug03G0245500 Rorug03G0295300 Rorug03G0295400 Rorug03G0295500 Rorug03G0295800 Rorug03G0296000 Rorug05G0158700 Rorug05G0158800 Rorug05G0158900 Rorug05G0159800 Rorug05G0159800 Rorug05G0170800 Rorug05G0170900 Rorug05G0170900 Rorug05G0211100 Rorug05G0211200 Rorug05G0246600 Rorug05G0328600 Rorug05G0367800 Rorug05G0518400 Rorug06G0018600 Rorug06G0055600 Rorug07G0250400 RorugMtG0003800.1 RorugMtG0003900.1 RorugMtG0004200.1
rosa_samantha Rh1AG007500 Rh1AG075300 Rh1AG075700 Rh1AG240100 Rh1BG060500 Rh1BG060600 Rh1BG061000 Rh1BG062200 Rh3BG217100 Rh3BG221000 Rh3BG221200 Rh3BG221600 Rh3BG222000 Rh3BG227500 Rh3DG212800 Rh3DG212900 Rh3DG216600 Rh3DG217100 Rh3DG217400 Rh5BG253400 Rh5BG261900 Rh5BG303800 Rh5BG399200 Rh5BG399300 Rh5BG399400 Rh5BG399600 Rh5BG405300 Rh5BG405400 Rh5BG441500 Rh5CG282400 Rh5CG285300 Rh5CG286100 Rh5CG290300 Rh5CG294000 Rh5CG331000 Rh5CG359600 Rh5CG423200 Rh5CG423300 Rh5CG423400 Rh5CG423700 Rh5CG429400 Rh5CG431900 Rh5CG464200 Rh5DG262000 Rh6AG030300 Rh6AG030500 Rh6AG030600 Rh6AG032900 Rh6AG140400 Rh6AG141200 Rh6AG270000 Rh6AG451900 Rh6CG137800 Rh6DG027700 Rh6DG124300 Rh6DG124700 Rh6DG166400 Rh6DG452500 Rh7BG383200 Rh7CG283600 Rh7CG421200 Rh7CG421400 Rh7CG421800
rosa_wichuraiana Rw0G002000 Rw0G009100 Rw0G012450 Rw0G015810 Rw1G005940 Rw1G006020 Rw1G006040 Rw1G006060 Rw1G013290 Rw3G017240 Rw3G017250 Rw3G017470 Rw3G017500 Rw3G017520 Rw5G022920 Rw5G023200 Rw5G023620 Rw5G023940 Rw5G024130 Rw5G024170 Rw5G027380 Rw5G027440 Rw5G036040 Rw5G036500 Rw5G036540 Rw5G036560 Rw5G037000 Rw5G037270 Rw5G040050 Rw6G002730 Rw6G012120 Rw6G012190 Rw6G014890 Rw6G017470 Rw6G039370 Rw7G022830 Rw7G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1283
AccI GTMKAC 1 cut(s) 301
AciI CCGC 2 cut(s) 491, 851
AclWI GGATC 2 cut(s) 1020, 1652
AcsI RAATTY 3 cut(s) 697, 1110, 1295
AcuI CTGAAG 4 cut(s) 245, 624, 999, 1154
AdeI CACNNNGTG 1 cut(s) 692
AfaI GTAC 3 cut(s) 347, 952, 1376
AfiI CCNNNNNNNGG 5 cut(s) 256, 501, 502, 1198, 1350
AflIII ACRYGT 2 cut(s) 660, 933
AgsI TTSAA 8 cut(s) 272, 412, 421, 442, 527, 866, 1076, 1471
AjiI CACGTC 1 cut(s) 218
AjnI CCWGG 2 cut(s) 291, 1145
AleI CACNNNNGTG 1 cut(s) 461
Alw21I GWGCWC 1 cut(s) 652
Alw26I GTCTC 5 cut(s) 610, 880, 1269, 1448, 1513
AlwI GGATC 2 cut(s) 1020, 1652
AlwNI CAGNNNCTG 2 cut(s) 26, 1541
Ama87I CYCGRG 2 cut(s) 557, 1569
AoxI GGCC 2 cut(s) 492, 1164
ApeKI GCWGC 7 cut(s) 23, 200, 203, 236, 1220, 1529, 1643
ApoI RAATTY 3 cut(s) 697, 1110, 1295
ArsI GACNNNNNNTTYG 2 cut(s) 951, 983
Asp700I GAANNNNTTC 2 cut(s) 57, 1512
AspLEI GCGC 2 cut(s) 66, 249
AspS9I GGNCC 1 cut(s) 493
AsuHPI GGTGA 2 cut(s) 350, 446
AsuII TTCGAA 2 cut(s) 957, 1337
AvaI CYCGRG 2 cut(s) 557, 1569
BauI CACGAG 1 cut(s) 816
BbsI GAAGAC 1 cut(s) 126
Bbv12I GWGCWC 1 cut(s) 652
BbvCI CCTCAGC 1 cut(s) 1161
BbvI GCAGC 7 cut(s) 35, 187, 190, 223, 1232, 1516, 1630
BccI CCATC 4 cut(s) 329, 504, 768, 1164
BciT130I CCWGG 2 cut(s) 293, 1147
BcoDI GTCTC 5 cut(s) 610, 880, 1269, 1448, 1513
BfaI CTAG 3 cut(s) 84, 611, 1410
BfoI RGCGCY 1 cut(s) 250
BglII AGATCT 1 cut(s) 1100
BisI GCNGC 8 cut(s) 24, 201, 204, 237, 492, 1221, 1530, 1644
BlsI GCNGC 8 cut(s) 25, 202, 205, 238, 493, 1222, 1531, 1645
Bme1390I CCNGG 2 cut(s) 293, 1147
BmeT110I CYCGRG 2 cut(s) 557, 1569
BmgBI CACGTC 1 cut(s) 218
BmgT120I GGNCC 1 cut(s) 493
BmiI GGNNCC 1 cut(s) 1175
BmrFI CCNGG 2 cut(s) 293, 1147
BmsI GCATC 7 cut(s) 187, 795, 931, 988, 1175, 1232, 1591
BpiI GAAGAC 1 cut(s) 126
BpmI CTGGAG 1 cut(s) 1518
Bpu10I CCTNAGC 1 cut(s) 1161
Bpu14I TTCGAA 2 cut(s) 957, 1337
BpuEI CTTGAG 1 cut(s) 117
Bsa29I ATCGAT 1 cut(s) 1065
BsaBI GATNNNNATC 1 cut(s) 339
BsaI GGTCTC 2 cut(s) 610, 1513
BsaJI CCNNGG 2 cut(s) 250, 291
BsaXI ACNNNNNCTCC 2 cut(s) 1516, 1546
Bsc4I CCNNNNNNNGG 5 cut(s) 256, 501, 502, 1198, 1350
Bse1I ACTGG 3 cut(s) 792, 1535, 1661
Bse3DI GCAATG 2 cut(s) 1365, 1653
Bse8I GATNNNNATC 1 cut(s) 339
BseBI CCWGG 2 cut(s) 293, 1147
BseCI ATCGAT 1 cut(s) 1065
BseDI CCNNGG 2 cut(s) 250, 291
BseGI GGATG 7 cut(s) 810, 934, 1003, 1063, 1190, 1207, 1366
BseJI GATNNNNATC 1 cut(s) 339
BseLI CCNNNNNNNGG 5 cut(s) 256, 501, 502, 1198, 1350
BseMI GCAATG 2 cut(s) 1365, 1653
BseMII CTCAG 5 cut(s) 388, 491, 772, 1152, 1508
BseNI ACTGG 3 cut(s) 792, 1535, 1661
BseRI GAGGAG 2 cut(s) 173, 1094
BseXI GCAGC 7 cut(s) 35, 187, 190, 223, 1232, 1516, 1630
BseYI CCCAGC 1 cut(s) 1370
BshFI GGCC 2 cut(s) 494, 1166
BshVI ATCGAT 1 cut(s) 1065
BsiHKAI GWGCWC 1 cut(s) 652
BsiHKCI CYCGRG 2 cut(s) 557, 1569
BsiSI CCGG 1 cut(s) 50
BslFI GGGAC 1 cut(s) 1523
BslI CCNNNNNNNGG 5 cut(s) 256, 501, 502, 1198, 1350
BsmAI GTCTC 5 cut(s) 610, 880, 1269, 1448, 1513
BsmFI GGGAC 1 cut(s) 1523
BsmI GAATGC 1 cut(s) 35
BsnI GGCC 2 cut(s) 494, 1166
Bso31I GGTCTC 2 cut(s) 610, 1513
BsoBI CYCGRG 2 cut(s) 557, 1569
Bsp119I TTCGAA 2 cut(s) 957, 1337
Bsp1286I GDGCHC 1 cut(s) 652
Bsp1407I TGTACA 1 cut(s) 345
Bsp143I GATC 7 cut(s) 36, 97, 340, 1012, 1100, 1579, 1657
BspACI CCGC 2 cut(s) 491, 851
BspANI GGCC 2 cut(s) 494, 1166
BspCNI CTCAG 5 cut(s) 387, 492, 771, 1153, 1509
BspDI ATCGAT 1 cut(s) 1065
BspHI TCATGA 1 cut(s) 325
BspLI GGNNCC 1 cut(s) 1175
BspPI GGATC 2 cut(s) 1020, 1652
BspQI GCTCTTC 1 cut(s) 363
BspT104I TTCGAA 2 cut(s) 957, 1337
BspTNI GGTCTC 2 cut(s) 610, 1513
BsrDI GCAATG 2 cut(s) 1365, 1653
BsrGI TGTACA 1 cut(s) 345
BsrI ACTGG 3 cut(s) 792, 1535, 1661
BssECI CCNNGG 2 cut(s) 250, 291
BssMI GATC 7 cut(s) 36, 97, 340, 1012, 1100, 1579, 1657
BssNAI GTATAC 1 cut(s) 302
BssSI CACGAG 1 cut(s) 816
Bst1107I GTATAC 1 cut(s) 302
Bst2BI CACGAG 1 cut(s) 816
Bst2UI CCWGG 2 cut(s) 293, 1147
Bst4CI ACNGT 2 cut(s) 692, 922
Bst6I CTCTTC 4 cut(s) 363, 522, 1071, 1503
BstAUI TGTACA 1 cut(s) 345
BstBI TTCGAA 2 cut(s) 957, 1337
BstDEI CTNAG 9 cut(s) 45, 374, 451, 500, 758, 770, 1161, 1328, 1517
BstF5I GGATG 7 cut(s) 810, 934, 1003, 1063, 1190, 1207, 1366
BstH2I RGCGCY 1 cut(s) 250
BstHHI GCGC 2 cut(s) 66, 249
BstKTI GATC 7 cut(s) 39, 100, 343, 1015, 1103, 1582, 1660
BstMAI GTCTC 5 cut(s) 610, 880, 1269, 1448, 1513
BstMBI GATC 7 cut(s) 36, 97, 340, 1012, 1100, 1579, 1657
BstMWI GCNNNNNNNGC 3 cut(s) 20, 376, 1090
BstNI CCWGG 2 cut(s) 293, 1147
BstNSI RCATGY 5 cut(s) 308, 664, 937, 1403, 1616
BstSCI CCNGG 2 cut(s) 291, 1145
BstV1I GCAGC 7 cut(s) 35, 187, 190, 223, 1232, 1516, 1630
BstV2I GAAGAC 1 cut(s) 126
BstX2I RGATCY 1 cut(s) 1100
BstYI RGATCY 1 cut(s) 1100
BstZ17I GTATAC 1 cut(s) 302
Bsu15I ATCGAT 1 cut(s) 1065
BsuRI GGCC 2 cut(s) 494, 1166
BsuTUI ATCGAT 1 cut(s) 1065
BtgZI GCGATG 1 cut(s) 417
BtrI CACGTC 1 cut(s) 218
BtsCI GGATG 7 cut(s) 810, 934, 1003, 1063, 1190, 1207, 1366
BtsI GCAGTG 1 cut(s) 1524
BtsIMutI CAGTG 7 cut(s) 192, 456, 697, 743, 766, 1524, 1668
CaiI CAGNNNCTG 2 cut(s) 26, 1541
CciI TCATGA 1 cut(s) 325
CfoI GCGC 2 cut(s) 66, 249
Cfr13I GGNCC 1 cut(s) 493
ClaI ATCGAT 1 cut(s) 1065
Csp6I GTAC 3 cut(s) 346, 951, 1375
CviQI GTAC 3 cut(s) 346, 951, 1375
DdeI CTNAG 9 cut(s) 45, 374, 451, 500, 758, 770, 1161, 1328, 1517
DpnI GATC 7 cut(s) 38, 99, 342, 1014, 1102, 1581, 1659
DpnII GATC 7 cut(s) 36, 97, 340, 1012, 1100, 1579, 1657
DraI TTTAAA 1 cut(s) 79
DraIII CACNNNGTG 1 cut(s) 692
Eam1104I CTCTTC 4 cut(s) 363, 522, 1071, 1503
EarI CTCTTC 4 cut(s) 363, 522, 1071, 1503
Eco31I GGTCTC 2 cut(s) 610, 1513
Eco32I GATATC 1 cut(s) 1465
Eco57I CTGAAG 4 cut(s) 245, 624, 999, 1154
Eco88I CYCGRG 2 cut(s) 557, 1569
EcoRI GAATTC 2 cut(s) 1110, 1295
EcoRII CCWGG 2 cut(s) 291, 1145
EcoRV GATATC 1 cut(s) 1465
EcoT22I ATGCAT 1 cut(s) 946
FalI AAGNNNNNCTT 2 cut(s) 434, 466
FaqI GGGAC 1 cut(s) 1523
FauI CCCGC 1 cut(s) 844
FblI GTMKAC 1 cut(s) 301
Fnu4HI GCNGC 8 cut(s) 24, 201, 204, 237, 492, 1221, 1530, 1644
FokI GGATG 7 cut(s) 817, 941, 1010, 1070, 1197, 1214, 1373
Fsp4HI GCNGC 8 cut(s) 24, 201, 204, 237, 492, 1221, 1530, 1644
FspBI CTAG 3 cut(s) 84, 611, 1410
GlaI GCGC 2 cut(s) 65, 248
GluI GCNGC 8 cut(s) 24, 201, 204, 237, 492, 1221, 1530, 1644
GsaI CCCAGC 1 cut(s) 1374
GsuI CTGGAG 1 cut(s) 1518
HaeII RGCGCY 1 cut(s) 250
HaeIII GGCC 2 cut(s) 494, 1166
HapII CCGG 1 cut(s) 50
HhaI GCGC 2 cut(s) 66, 249
Hin6I GCGC 2 cut(s) 64, 247
HinP1I GCGC 2 cut(s) 64, 247
HincII GTYRAC 1 cut(s) 221
HindII GTYRAC 1 cut(s) 221
HindIII AAGCTT 3 cut(s) 448, 681, 902
HinfI GANTC 4 cut(s) 209, 560, 1138, 1487
HpaII CCGG 1 cut(s) 50
HphI GGTGA 2 cut(s) 350, 446
Hpy166II GTNNAC 4 cut(s) 105, 221, 302, 743
Hpy188I TCNGA 6 cut(s) 501, 1134, 1447, 1518, 1619, 1673
Hpy188III TCNNGA 8 cut(s) 317, 326, 442, 557, 674, 898, 973, 1055
Hpy8I GTNNAC 4 cut(s) 105, 221, 302, 743
Hpy99I CGWCG 1 cut(s) 1256
HpyAV CCTTC 4 cut(s) 175, 1175, 1489, 1615
HpyCH4III ACNGT 2 cut(s) 692, 922
HpyCH4IV ACGT 3 cut(s) 217, 298, 876
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 376, 1090
HpyF3I CTNAG 9 cut(s) 45, 374, 451, 500, 758, 770, 1161, 1328, 1517
HpySE526I ACGT 3 cut(s) 217, 298, 876
HspAI GCGC 2 cut(s) 64, 247
Kzo9I GATC 7 cut(s) 36, 97, 340, 1012, 1100, 1579, 1657
LguI GCTCTTC 1 cut(s) 363
LmnI GCTCC 3 cut(s) 160, 1081, 1537
Lsp1109I GCAGC 7 cut(s) 35, 187, 190, 223, 1232, 1516, 1630
LweI GCATC 7 cut(s) 187, 795, 931, 988, 1175, 1232, 1591
MaeI CTAG 3 cut(s) 84, 611, 1410
MaeII ACGT 3 cut(s) 217, 298, 876
MaeIII GTNAC 6 cut(s) 434, 454, 656, 761, 916, 1235
MalI GATC 7 cut(s) 38, 99, 342, 1014, 1102, 1581, 1659
MboI GATC 7 cut(s) 36, 97, 340, 1012, 1100, 1579, 1657
MfeI CAATTG 2 cut(s) 984, 1458
MflI RGATCY 1 cut(s) 1100
MhlI GDGCHC 1 cut(s) 652
MluCI AATT 8 cut(s) 231, 697, 716, 984, 1110, 1295, 1458, 1479
MlyI GAGTC 1 cut(s) 569
MmeI TCCRAC 1 cut(s) 1597
Mph1103I ATGCAT 1 cut(s) 946
MroXI GAANNNNTTC 2 cut(s) 57, 1512
MseI TTAA 4 cut(s) 78, 726, 1548, 1560
MslI CAYNNNNRTG 2 cut(s) 330, 461
MspA1I CMGCKG 1 cut(s) 26
MspI CCGG 1 cut(s) 50
MspR9I CCNGG 2 cut(s) 293, 1147
MunI CAATTG 2 cut(s) 984, 1458
Mva1269I GAATGC 1 cut(s) 35
MvaI CCWGG 2 cut(s) 293, 1147
MwoI GCNNNNNNNGC 3 cut(s) 20, 376, 1090
NdeII GATC 7 cut(s) 36, 97, 340, 1012, 1100, 1579, 1657
NlaIV GGNNCC 1 cut(s) 1175
NmeAIII GCCGAG 1 cut(s) 631
NmuCI GTSAC 3 cut(s) 434, 454, 761
NsiI ATGCAT 1 cut(s) 946
NspI RCATGY 5 cut(s) 308, 664, 937, 1403, 1616
NspV TTCGAA 2 cut(s) 957, 1337
OliI CACNNNNGTG 1 cut(s) 461
PaeR7I CTCGAG 1 cut(s) 557
PagI TCATGA 1 cut(s) 325
PciI ACATGT 2 cut(s) 660, 933
PciSI GCTCTTC 1 cut(s) 363
PcsI WCGNNNNNNNCGW 1 cut(s) 963
PctI GAATGC 1 cut(s) 35
PdmI GAANNNNTTC 2 cut(s) 57, 1512
PfeI GAWTC 3 cut(s) 209, 1138, 1487
PkrI GCNGC 8 cut(s) 25, 202, 205, 238, 493, 1222, 1531, 1645
PleI GAGTC 1 cut(s) 568
PpsI GAGTC 1 cut(s) 568
PscI ACATGT 2 cut(s) 660, 933
PsiI TTATAA 1 cut(s) 1283
Psp6I CCWGG 2 cut(s) 291, 1145
PspFI CCCAGC 1 cut(s) 1370
PspGI CCWGG 2 cut(s) 291, 1145
PspN4I GGNNCC 1 cut(s) 1175
PspPI GGNCC 1 cut(s) 493
PstNI CAGNNNCTG 2 cut(s) 26, 1541
PsuI RGATCY 1 cut(s) 1100
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 3 cut(s) 347, 952, 1376
RsaNI GTAC 3 cut(s) 346, 951, 1375
RseI CAYNNNNRTG 2 cut(s) 330, 461
SapI GCTCTTC 1 cut(s) 363
SaqAI TTAA 4 cut(s) 78, 726, 1548, 1560
SatI GCNGC 8 cut(s) 24, 201, 204, 237, 492, 1221, 1530, 1644
Sau3AI GATC 7 cut(s) 36, 97, 340, 1012, 1100, 1579, 1657
Sau96I GGNCC 1 cut(s) 493
SchI GAGTC 1 cut(s) 569
ScrFI CCNGG 2 cut(s) 293, 1147
SduI GDGCHC 1 cut(s) 652
SfaNI GCATC 7 cut(s) 187, 795, 931, 988, 1175, 1232, 1591
Sfr274I CTCGAG 1 cut(s) 557
SfuI TTCGAA 2 cut(s) 957, 1337
SlaI CTCGAG 1 cut(s) 557
SmiMI CAYNNNNRTG 2 cut(s) 330, 461
SmlI CTYRAG 2 cut(s) 132, 557
SmoI CTYRAG 2 cut(s) 132, 557
Sse9I AATT 8 cut(s) 231, 697, 716, 984, 1110, 1295, 1458, 1479
SsiI CCGC 2 cut(s) 491, 851
SspMI CTAG 3 cut(s) 84, 611, 1410
StyD4I CCNGG 2 cut(s) 291, 1145
TaaI ACNGT 2 cut(s) 692, 922
TaiI ACGT 3 cut(s) 220, 301, 879
TaqI TCGA 5 cut(s) 558, 957, 1065, 1299, 1337
TasI AATT 8 cut(s) 231, 697, 716, 984, 1110, 1295, 1458, 1479
TatI WGTACW 1 cut(s) 345
TauI GCSGC 1 cut(s) 494
TfiI GAWTC 3 cut(s) 209, 1138, 1487
Tru1I TTAA 4 cut(s) 78, 726, 1548, 1560
Tru9I TTAA 4 cut(s) 78, 726, 1548, 1560
TscAI CASTG 7 cut(s) 199, 463, 697, 743, 766, 1531, 1668
TseFI GTSAC 3 cut(s) 434, 454, 761
TseI GCWGC 7 cut(s) 23, 200, 203, 236, 1220, 1529, 1643
Tsp45I GTSAC 3 cut(s) 434, 454, 761
TspDTI ATGAA 6 cut(s) 314, 693, 1083, 1442, 1446, 1643
TspGWI ACGGA 1 cut(s) 1240
TspRI CASTG 7 cut(s) 199, 463, 697, 743, 766, 1531, 1668
XapI RAATTY 3 cut(s) 697, 1110, 1295
XceI RCATGY 5 cut(s) 308, 664, 937, 1403, 1616
XhoI CTCGAG 1 cut(s) 557
XmiI GTMKAC 1 cut(s) 301
XmnI GAANNNNTTC 2 cut(s) 57, 1512
XspI CTAG 3 cut(s) 84, 611, 1410
Zsp2I ATGCAT 1 cut(s) 946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.