(-)-alpha-pinene synthase-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 185 |
| AccII | CGCG | 2 cut(s) | 92, 392 |
| AclWI | GGATC | 2 cut(s) | 534, 547 |
| AcoI | YGGCCR | 1 cut(s) | 553 |
| AcyI | GRCGYC | 1 cut(s) | 333 |
| AfaI | GTAC | 3 cut(s) | 149, 588, 658 |
| AfeI | AGCGCT | 2 cut(s) | 104, 470 |
| AjiI | CACGTC | 1 cut(s) | 716 |
| AjnI | CCWGG | 1 cut(s) | 291 |
| Alw21I | GWGCWC | 1 cut(s) | 419 |
| AlwI | GGATC | 2 cut(s) | 534, 547 |
| Aor51HI | AGCGCT | 2 cut(s) | 104, 470 |
| AoxI | GGCC | 6 cut(s) | 3, 113, 304, 450, 553, 728 |
| ApeKI | GCWGC | 5 cut(s) | 245, 270, 471, 489, 492 |
| AspLEI | GCGC | 3 cut(s) | 105, 471, 509 |
| AspS9I | GGNCC | 5 cut(s) | 304, 405, 592, 633, 728 |
| AsuC2I | CCSGG | 4 cut(s) | 242, 377, 445, 637 |
| AsuHPI | GGTGA | 1 cut(s) | 376 |
| AsuII | TTCGAA | 1 cut(s) | 51 |
| AsuNHI | GCTAGC | 1 cut(s) | 314 |
| AvaII | GGWCC | 3 cut(s) | 405, 592, 633 |
| BamHI | GGATCC | 1 cut(s) | 539 |
| BanI | GGYRCC | 1 cut(s) | 185 |
| BauI | CACGAG | 2 cut(s) | 177, 410 |
| BbsI | GAAGAC | 2 cut(s) | 60, 155 |
| Bbv12I | GWGCWC | 1 cut(s) | 419 |
| BbvI | GCAGC | 5 cut(s) | 232, 282, 458, 501, 504 |
| BccI | CCATC | 1 cut(s) | 428 |
| BceAI | ACGGC | 3 cut(s) | 332, 494, 568 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 262, 296 |
| BciT130I | CCWGG | 1 cut(s) | 293 |
| BcnI | CCSGG | 4 cut(s) | 242, 377, 445, 637 |
| BfaI | CTAG | 1 cut(s) | 315 |
| BfmI | CTRYAG | 2 cut(s) | 213, 268 |
| BfoI | RGCGCY | 3 cut(s) | 106, 472, 510 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 11 |
| BlpI | GCTNAGC | 1 cut(s) | 609 |
| BmcAI | AGTACT | 1 cut(s) | 658 |
| Bme1390I | CCNGG | 5 cut(s) | 242, 293, 377, 445, 637 |
| Bme18I | GGWCC | 3 cut(s) | 405, 592, 633 |
| BmgBI | CACGTC | 1 cut(s) | 716 |
| BmgT120I | GGNCC | 5 cut(s) | 304, 405, 592, 633, 728 |
| BmiI | GGNNCC | 6 cut(s) | 187, 286, 305, 407, 541, 729 |
| BmrFI | CCNGG | 5 cut(s) | 242, 293, 377, 445, 637 |
| BmsI | GCATC | 2 cut(s) | 244, 687 |
| BmtI | GCTAGC | 1 cut(s) | 318 |
| BpiI | GAAGAC | 2 cut(s) | 60, 155 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 550, 582 |
| BpmI | CTGGAG | 1 cut(s) | 529 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 609 |
| Bpu14I | TTCGAA | 1 cut(s) | 51 |
| BpuEI | CTTGAG | 1 cut(s) | 645 |
| BpuMI | CCSGG | 4 cut(s) | 242, 377, 445, 637 |
| BsaHI | GRCGYC | 1 cut(s) | 333 |
| BsaJI | CCNNGG | 3 cut(s) | 240, 453, 550 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 323, 353, 401, 431 |
| Bse1I | ACTGG | 1 cut(s) | 594 |
| BseBI | CCWGG | 1 cut(s) | 293 |
| BseDI | CCNNGG | 3 cut(s) | 240, 453, 550 |
| BseGI | GGATG | 1 cut(s) | 235 |
| BseMII | CTCAG | 3 cut(s) | 581, 600, 723 |
| BseNI | ACTGG | 1 cut(s) | 594 |
| BseRI | GAGGAG | 2 cut(s) | 158, 695 |
| BseXI | GCAGC | 5 cut(s) | 232, 282, 458, 501, 504 |
| BsgI | GTGCAG | 1 cut(s) | 707 |
| Bsh1236I | CGCG | 2 cut(s) | 92, 392 |
| Bsh1285I | CGRYCG | 1 cut(s) | 444 |
| BshFI | GGCC | 6 cut(s) | 5, 115, 306, 452, 555, 730 |
| BshNI | GGYRCC | 1 cut(s) | 185 |
| BsiEI | CGRYCG | 1 cut(s) | 444 |
| BsiHKAI | GWGCWC | 1 cut(s) | 419 |
| BsiSI | CCGG | 6 cut(s) | 242, 377, 444, 614, 636, 701 |
| BsnI | GGCC | 6 cut(s) | 5, 115, 306, 452, 555, 730 |
| Bsp119I | TTCGAA | 1 cut(s) | 51 |
| Bsp1286I | GDGCHC | 1 cut(s) | 419 |
| Bsp143I | GATC | 1 cut(s) | 539 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 609 |
| BspANI | GGCC | 6 cut(s) | 5, 115, 306, 452, 555, 730 |
| BspCNI | CTCAG | 3 cut(s) | 580, 601, 724 |
| BspFNI | CGCG | 2 cut(s) | 92, 392 |
| BspLI | GGNNCC | 6 cut(s) | 187, 286, 305, 407, 541, 729 |
| BspMAI | CTGCAG | 1 cut(s) | 272 |
| BspOI | GCTAGC | 1 cut(s) | 318 |
| BspPI | GGATC | 2 cut(s) | 534, 547 |
| BspT104I | TTCGAA | 1 cut(s) | 51 |
| BspT107I | GGYRCC | 1 cut(s) | 185 |
| BsrI | ACTGG | 1 cut(s) | 594 |
| BssECI | CCNNGG | 3 cut(s) | 240, 453, 550 |
| BssMI | GATC | 1 cut(s) | 539 |
| BssNI | GRCGYC | 1 cut(s) | 333 |
| BssSI | CACGAG | 2 cut(s) | 177, 410 |
| BssT1I | CCWWGG | 1 cut(s) | 453 |
| Bst2BI | CACGAG | 2 cut(s) | 177, 410 |
| Bst2UI | CCWGG | 1 cut(s) | 293 |
| Bst4CI | ACNGT | 4 cut(s) | 323, 361, 485, 520 |
| Bst6I | CTCTTC | 2 cut(s) | 135, 647 |
| BstACI | GRCGYC | 1 cut(s) | 333 |
| BstBI | TTCGAA | 1 cut(s) | 51 |
| BstC8I | GCNNGC | 6 cut(s) | 12, 174, 316, 450, 575, 606 |
| BstDEI | CTNAG | 3 cut(s) | 567, 609, 732 |
| BstDSI | CCRYGG | 1 cut(s) | 550 |
| BstF5I | GGATG | 1 cut(s) | 235 |
| BstFNI | CGCG | 2 cut(s) | 92, 392 |
| BstH2I | RGCGCY | 3 cut(s) | 106, 472, 510 |
| BstHHI | GCGC | 3 cut(s) | 105, 471, 509 |
| BstKTI | GATC | 1 cut(s) | 542 |
| BstMBI | GATC | 1 cut(s) | 539 |
| BstMCI | CGRYCG | 1 cut(s) | 444 |
| BstNI | CCWGG | 1 cut(s) | 293 |
| BstSCI | CCNGG | 5 cut(s) | 240, 291, 375, 443, 635 |
| BstSFI | CTRYAG | 2 cut(s) | 213, 268 |
| BstUI | CGCG | 2 cut(s) | 92, 392 |
| BstV1I | GCAGC | 5 cut(s) | 232, 282, 458, 501, 504 |
| BstV2I | GAAGAC | 2 cut(s) | 60, 155 |
| BstX2I | RGATCY | 1 cut(s) | 539 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 299 |
| BstYI | RGATCY | 1 cut(s) | 539 |
| BsuRI | GGCC | 6 cut(s) | 5, 115, 306, 452, 555, 730 |
| BtgI | CCRYGG | 1 cut(s) | 550 |
| BtrI | CACGTC | 1 cut(s) | 716 |
| BtsCI | GGATG | 1 cut(s) | 235 |
| Cac8I | GCNNGC | 6 cut(s) | 12, 174, 316, 450, 575, 606 |
| CfoI | GCGC | 3 cut(s) | 105, 471, 509 |
| Cfr13I | GGNCC | 5 cut(s) | 304, 405, 592, 633, 728 |
| CseI | GACGC | 1 cut(s) | 341 |
| Csp6I | GTAC | 3 cut(s) | 148, 587, 657 |
| CviAII | CATG | 2 cut(s) | 73, 151 |
| CviQI | GTAC | 3 cut(s) | 148, 587, 657 |
| DdeI | CTNAG | 3 cut(s) | 567, 609, 732 |
| DpnI | GATC | 1 cut(s) | 541 |
| DpnII | GATC | 1 cut(s) | 539 |
| EaeI | YGGCCR | 1 cut(s) | 553 |
| Eam1104I | CTCTTC | 2 cut(s) | 135, 647 |
| EarI | CTCTTC | 2 cut(s) | 135, 647 |
| EciI | GGCGGA | 2 cut(s) | 331, 658 |
| Eco130I | CCWWGG | 1 cut(s) | 453 |
| Eco147I | AGGCCT | 2 cut(s) | 115, 452 |
| Eco47I | GGWCC | 3 cut(s) | 405, 592, 633 |
| Eco47III | AGCGCT | 2 cut(s) | 104, 470 |
| EcoO109I | RGGNCCY | 2 cut(s) | 405, 728 |
| EcoRII | CCWGG | 1 cut(s) | 291 |
| EcoT14I | CCWWGG | 1 cut(s) | 453 |
| ErhI | CCWWGG | 1 cut(s) | 453 |
| FaeI | CATG | 2 cut(s) | 76, 154 |
| FaiI | YATR | 2 cut(s) | 74, 152 |
| FatI | CATG | 2 cut(s) | 72, 150 |
| FokI | GGATG | 1 cut(s) | 222 |
| FspBI | CTAG | 1 cut(s) | 315 |
| GlaI | GCGC | 3 cut(s) | 104, 470, 508 |
| GsuI | CTGGAG | 1 cut(s) | 529 |
| HaeII | RGCGCY | 3 cut(s) | 106, 472, 510 |
| HaeIII | GGCC | 6 cut(s) | 5, 115, 306, 452, 555, 730 |
| HapII | CCGG | 6 cut(s) | 242, 377, 444, 614, 636, 701 |
| HgaI | GACGC | 1 cut(s) | 341 |
| HhaI | GCGC | 3 cut(s) | 105, 471, 509 |
| Hin1I | GRCGYC | 1 cut(s) | 333 |
| Hin1II | CATG | 2 cut(s) | 76, 154 |
| Hin6I | GCGC | 3 cut(s) | 103, 469, 507 |
| HinP1I | GCGC | 3 cut(s) | 103, 469, 507 |
| HinfI | GANTC | 3 cut(s) | 128, 565, 691 |
| HpaII | CCGG | 6 cut(s) | 242, 377, 444, 614, 636, 701 |
| HphI | GGTGA | 1 cut(s) | 376 |
| Hpy166II | GTNNAC | 1 cut(s) | 633 |
| Hpy188I | TCNGA | 6 cut(s) | 139, 331, 526, 570, 696, 720 |
| Hpy188III | TCNNGA | 3 cut(s) | 364, 439, 514 |
| Hpy8I | GTNNAC | 1 cut(s) | 633 |
| Hpy99I | CGWCG | 2 cut(s) | 485, 520 |
| HpyAV | CCTTC | 1 cut(s) | 521 |
| HpyCH4III | ACNGT | 4 cut(s) | 323, 361, 485, 520 |
| HpyCH4IV | ACGT | 2 cut(s) | 97, 715 |
| HpyCH4V | TGCA | 3 cut(s) | 10, 270, 688 |
| HpyF3I | CTNAG | 3 cut(s) | 567, 609, 732 |
| HpySE526I | ACGT | 2 cut(s) | 97, 715 |
| Hsp92I | GRCGYC | 1 cut(s) | 333 |
| Hsp92II | CATG | 2 cut(s) | 76, 154 |
| HspAI | GCGC | 3 cut(s) | 103, 469, 507 |
| Kzo9I | GATC | 1 cut(s) | 539 |
| LmnI | GCTCC | 3 cut(s) | 110, 290, 422 |
| Lsp1109I | GCAGC | 5 cut(s) | 232, 282, 458, 501, 504 |
| LweI | GCATC | 2 cut(s) | 244, 687 |
| MaeI | CTAG | 1 cut(s) | 315 |
| MaeII | ACGT | 2 cut(s) | 97, 715 |
| MaeIII | GTNAC | 2 cut(s) | 382, 511 |
| MalI | GATC | 1 cut(s) | 541 |
| MboI | GATC | 1 cut(s) | 539 |
| MboII | GAAGA | 6 cut(s) | 65, 152, 155, 661, 664, 718 |
| MflI | RGATCY | 1 cut(s) | 539 |
| MhlI | GDGCHC | 1 cut(s) | 419 |
| MlyI | GAGTC | 3 cut(s) | 122, 559, 700 |
| MspI | CCGG | 6 cut(s) | 242, 377, 444, 614, 636, 701 |
| MspR9I | CCNGG | 5 cut(s) | 242, 293, 377, 445, 637 |
| MvaI | CCWGG | 1 cut(s) | 293 |
| MvnI | CGCG | 2 cut(s) | 92, 392 |
| NciI | CCSGG | 4 cut(s) | 242, 377, 445, 637 |
| NdeII | GATC | 1 cut(s) | 539 |
| NheI | GCTAGC | 1 cut(s) | 314 |
| NlaIII | CATG | 2 cut(s) | 76, 154 |
| NlaIV | GGNNCC | 6 cut(s) | 187, 286, 305, 407, 541, 729 |
| NmeAIII | GCCGAG | 2 cut(s) | 21, 697 |
| NmuCI | GTSAC | 2 cut(s) | 382, 511 |
| NspV | TTCGAA | 1 cut(s) | 51 |
| PceI | AGGCCT | 2 cut(s) | 115, 452 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 48 |
| PleI | GAGTC | 3 cut(s) | 122, 559, 699 |
| PpsI | GAGTC | 3 cut(s) | 122, 559, 699 |
| PpuMI | RGGWCCY | 1 cut(s) | 405 |
| Psp5II | RGGWCCY | 1 cut(s) | 405 |
| Psp6I | CCWGG | 1 cut(s) | 291 |
| PspGI | CCWGG | 1 cut(s) | 291 |
| PspN4I | GGNNCC | 6 cut(s) | 187, 286, 305, 407, 541, 729 |
| PspPI | GGNCC | 5 cut(s) | 304, 405, 592, 633, 728 |
| PspPPI | RGGWCCY | 1 cut(s) | 405 |
| PstI | CTGCAG | 1 cut(s) | 272 |
| PsuI | RGATCY | 1 cut(s) | 539 |
| RsaI | GTAC | 3 cut(s) | 149, 588, 658 |
| RsaNI | GTAC | 3 cut(s) | 148, 587, 657 |
| Sau3AI | GATC | 1 cut(s) | 539 |
| Sau96I | GGNCC | 5 cut(s) | 304, 405, 592, 633, 728 |
| ScaI | AGTACT | 1 cut(s) | 658 |
| SchI | GAGTC | 3 cut(s) | 122, 559, 700 |
| ScrFI | CCNGG | 5 cut(s) | 242, 293, 377, 445, 637 |
| SduI | GDGCHC | 1 cut(s) | 419 |
| SetI | ASST | 7 cut(s) | 87, 100, 260, 341, 597, 687, 718 |
| SfaNI | GCATC | 2 cut(s) | 244, 687 |
| SfcI | CTRYAG | 2 cut(s) | 213, 268 |
| SfuI | TTCGAA | 1 cut(s) | 51 |
| SinI | GGWCC | 3 cut(s) | 405, 592, 633 |
| SmlI | CTYRAG | 1 cut(s) | 660 |
| SmoI | CTYRAG | 1 cut(s) | 660 |
| SseBI | AGGCCT | 2 cut(s) | 115, 452 |
| SspMI | CTAG | 1 cut(s) | 315 |
| StuI | AGGCCT | 2 cut(s) | 115, 452 |
| StyD4I | CCNGG | 5 cut(s) | 240, 291, 375, 443, 635 |
| StyI | CCWWGG | 1 cut(s) | 453 |
| TaaI | ACNGT | 4 cut(s) | 323, 361, 485, 520 |
| TaiI | ACGT | 2 cut(s) | 100, 718 |
| TaqI | TCGA | 3 cut(s) | 51, 123, 642 |
| TatI | WGTACW | 2 cut(s) | 147, 656 |
| TseFI | GTSAC | 2 cut(s) | 382, 511 |
| TseI | GCWGC | 5 cut(s) | 245, 270, 471, 489, 492 |
| Tsp45I | GTSAC | 2 cut(s) | 382, 511 |
| TspDTI | ATGAA | 1 cut(s) | 89 |
| VpaK11BI | GGWCC | 3 cut(s) | 405, 592, 633 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 482 |
| XspI | CTAG | 1 cut(s) | 315 |
| ZrmI | AGTACT | 1 cut(s) | 658 |